shift in adaptive immune response types in dry-eye disease
DESCRIPTION
Shift in Adaptive Immune Response Types in Dry-Eye Disease. Yihe Chen, Sunil K Chauhan, Reza Dana Schepens Eye Research Institute Harvard Medical School Boston, MA. The authors have no financial interest in the subject matter of this poster. Background. Dry eye disease (DED) - PowerPoint PPT PresentationTRANSCRIPT
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Shift in Adaptive Immune Response Types in Dry-Eye Disease
Yihe Chen, Sunil K Chauhan, Reza DanaSchepens Eye Research Institute
Harvard Medical SchoolBoston, MA
The authors have no financial interest in the subject matter of this poster
![Page 2: Shift in Adaptive Immune Response Types in Dry-Eye Disease](https://reader036.vdocuments.us/reader036/viewer/2022062520/568159e9550346895dc73534/html5/thumbnails/2.jpg)
Background
• Dry eye disease (DED)– An inflammatory autoimmune disorder– T cell involvement in the pathogenesis
Characterization of adaptive immune response during DED course would be relevant to the development of more effective therapies
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Purpose
Determine kinetics of generation of adaptive immunity in DED
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Methods
[Controlled-environment chamber]
DED induction
2 or 7 days
Conjunctiva
Cervical lymph nodesTissue harvest
PCR FCM
Analysis of immune
response
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ResultsIFN-IFN-γγ and IL-17 mRNA levels and IL-17 mRNA levels
Conjunctiva Draining lymph nodes
0
1
2
3
Day 2 Day7
Fo
ld c
ha
ng
e i
n m
RN
A e
xp
res
sio
n
IFN-γ
IL-17
0
1
2
3
Day 2 Day7
Fo
ld c
ha
ng
e i
n m
RN
A e
xp
res
sio
n
IFN-γ
IL-17
NL NL
NL: mRNA level in normal mice; * p < 0.05 compared with normal
* * *
*
Both the conjunctiva and draining lymph nodes of DED mice showed an enhanced expression of IFN-γ mRNA on day 2 while a substantially increased expression of IL-17 mRNA on day 7
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ResultsTh1 and Th17 cellsTh1 and Th17 cells
CD4
IL-1
7
Th1/17 cell is defined as IFN-γ+ / IL-17+ CD4+ population; * p < 0.05 compared with normal
Day 2 Day 7
IFN
-γ
Th1
Th17
*
*
•Th1 cells: significantly increased on day 2, and then diminished to the baseline level on day 7
•Th17 cells: no significant change on day 2, but significantly increased on day 7
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Conclusion
These findings suggest:
- the adaptive immune response in early DED is characterized by a Th1 phenotype, whereas,
-the progression of DED is characterized by the Th17 response