session4 ahmed jahoor - hem - nordgen jahoor nordic seed denmark. applying wild barley ......
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TertiaryOther wild barley
Gene pools of Hordeum
SecondaryH. bulbosum
PrimaryCultivated barleyH. spontaneum
Diversity indices within Hordeum vulgare,H. spontaneum and other wild barley species(based on 32 RFLP markers and 223 lines )
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H)
vulgare spontaneum others
Screening of H.vulgare ssp. spontaneum against European and Israeli powdery mildew isolates
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R-R R-I I-I R-S S-S
EuropeanIsraeli
Identified and localised new powdery mildew resistance genes with molecular markers in 41 H. vulgare ssp. Spontaneum derived lines
Locus Chromosome Number of alleles
Mla 1H 17
Mli 1H 1
Mlf 7H 7
Mlt 7H 3
Mlj 5H 1
Mlp ? 3
Localisation of qualitative and quantitative inherited mildew resistance gene with their respective number of alleles
italic → localised in H. spontaneum
Distribution of advance lines containing new sources of resistance for winter barley to German breeders
Line Year of distribution
Donor Number of backcrosses
Resistance locus
WI-1 1988 Rs-142-29 4 Mla
WI-2 1988 RS20-2 3 Unknown
WI-3 1989 L74-11 4 Unknown
WI-4 1989 RS2-2 4 Mla
WI-5 1989 RS111-1 4 Unknown
WI-6 1992 RS132-45 4 Not Mla
WI-7 1992 RS122-19 4 Unknown
WI-8 1992 RS170-A-S 4 Unknown
WI-9 1996 RS70-29 4 Mla
WI-10 1996 RS70-30 4 Unknown
Distribution of lines containing new sources of mildew resistance for spring barley to German breeders
Line Year ofdistribution
Donor Number ofbackcrosses
Resistancelocus
SI-1 1987 RS1-12 5 Mla
SI-2 1987 RS81-B-10 5 Unknown
SI-3 1989 1-B-86 5 Mla
SI-4 1989 1-B-87 4 Mlf+mlt
SI-5 1992 RS160-47 5 Linked withMla
SI-6 1992 RS-164-6 5 Mlf+mlt
SI-7 1992 RS14-C-24 4 Mla
SI-8 1996 RS42-8 5 Mli
SI-9 1996 RS42-6 5 Mlt
SI-10 1996 RS137-28 5 Mlf
SI-11 1996 HSY-78 5 Mlj
TILLING procedure
DNA
Isolation
Pooling
Gene specific primer pairs(2 different dyes)
PCR reaction
Denaturation
Renaturation
+ Cel I
Wt Mu Wt Mu
Spectrum of observed fragmentsfor 28 different Mla alleles
D*1B54A Mla16 105 163 222 116 171 343 347 363
RS170-47*Kieb Mla17 105 163 222 91 171 237 313 326 343 347 358 374 415
RS20-1*K.B Mla18 105 163 222 157 171 237 313 343 347 365 374 415
D*1B-86B Mla19 105 163 222 116 171 313 343 347 363
RS145-39*K Mla20 105 163 222 171 313 343 374 398
RS170-10*P Mla25 105 163 222 88 171 300 313 343 374 398
D*1B-20 Mla26 105 222 171 326 343 347 358 365 374 398 400
RS1-8*P.E Mla27 105 222 171 313 326 343 365 374 400 415
D*1B-151 Mla28 150 163 222 171 343 347 363 415
110-4*S Mla29 105 163 222 116 171 313 343 347 374 419
Turkey290 Mla31 105 163 222 91 116 171 237 313 343 347 374 415
RS90-13 Mla33 105 222 88 171 300 313 343 347 363 365 398 415
RS70-29xSxCxSxH Mla34 105 163 222 171 313 343 374 415
RS2-2xPicc. Mla35 105 163 222 126 171 313 343 374
P04A Mla7, Ml-k 105 163 222 88 171 300 313 343 347 374 415
Pallas Mla8 105 163 222 171 313 343 347 374 415
P08B Mla9 105 163 222 171 286 313 343 347 374 411
P07 Mla9, Ml-k 105 163 222 88 171 300 313 343 347 374 415
OPTIC Mla12 105 163 222 88 171 300 313 343 347 374 415
D*1B54A Mla16 105 163 222 116 171 343 347 363
RS170-47*Kieb Mla17 105 163 222 91 171 237 313 326 343 347 358 374 415
RS20-1*K.B Mla18 105 163 222 157 171 237 313 343 347 365 374 415
D*1B-86B Mla19 105 163 222 116 171 313 343 347 363
RS145-39*K Mla20 105 163 222 171 313 343 374 398
RS170-10*P Mla25 105 163 222 88 171 300 313 343 374 398
D*1B-20 Mla26 105 222 171 326 343 347 358 365 374 398 400
RS1-8*P.E Mla27 105 222 171 313 326 343 365 374 400 415
D*1B-151 Mla28 150 163 222 171 343 347 363 415
110-4*S Mla29 105 163 222 116 171 313 343 347 374 419
Marker for qualitative traits
std. susc. res. res.susc.
STS marker for barley powderymildew resistance gene Mla12developed from AFLP marker
std. std.res.uncl.
susc.ScrFI
susc.uncl.
res.ScrFI
SCAR marker for barley powderymildew resistance gene Mla12developed from RFLP marker
Released varieties developed with the new sources o f resistance against powdery mildew originating from H.
spontaneumSally Streng 1999 SI-1Roxana Breun 1998 1-B-53Vrena Nordsaat 2000 WI-1Havanna Breun 2000 1-B-53Alabama Lochow-Petkus 2000 SI-8Philadelphia Lochow-Petkus 2001 SI-8Marnie Breun 2002 1-B-80Venezia Bauer 2002 WI-1Denise Breun 2003 1-B-53Antalya Breun 2004 WI-7Djamila Nordsaat 2003 SI-4Grace Dr. J. Ackermann 2008 1-B-53Henrike Nordsaat 2007 1-B-53Isotta Breun 2004 1-B-53Iron Nordic Seed 2008 1-B-53
Leaf rust, powdery mildew and scald resistance genes on barley chromosome 2H 20 cM
S LC
[Rph1] MlLa
qLr1 qLr2 qLr3 qRh1
qPm3
Name
Population
LODEffectres. ParentVar.expl.
Krona xHOR106310.92.13 UnitsHOR106311%
qLr1
Krona xHOR10634.41.6 UnitsHOR106324%
qLr2
Krona xHOR10633.50.5 UnitsHOR10632%
qLr3
Igri xDanilo16.91.1 pointsIgri32%
qRh1
1B-87 xVada3.814 UnitsVada6%
qPm3
Leaf rust, powdery mildew and scald resistance genes on barley chromosome 5H
S C LRph2 RphQ Rph9 Rph12
20 cM
Name
Population
LODEffectres. ParentVar.expl.
1B-87 xVada5.919Vada9%
qLr6
1B-87 xVada15.952Vada41%
qLr5
1B-87 xVada6.517 unitsVada7%
qPm6
qLr5 qPm7
qPm6qLr6
qLr7
1B-87 xVada7.718 units1B-879%
qPm7
1B-87 xVada7.0151B-8741%
qLr7
Mlj
QTL analysis of powdery mildew resistance in a cross between wild barley and cultivated barley
m32p31-040m59e41-0410m48e38-2315MWG36MWG219720m31p31-0826m47e38-1336m32p31-1744MWG7549MWG80064MWG5567m59e41-0671S-921773S-11275BMag211m59e36-1277m47e41-0279m47e38-1285m47e38-1987BMac13101MWG943106m47e32-19112MWG518118BMag382127cMWG695132cMWG706a135m48e32-08137m48e38-20149
m47e41-14171m41p31-12174MWG2028177m33p31-11182MWG912188m31p31-07189m47e38-14190
1H
S-T650S-133-23S-2366cMWG68211m41p31-0215BMac13423
m48e32-0336m47e32-0643
m32p31-1153
MWG6463m42p31-0669
2H
m31p31-250S-92046m31p31-1811MWG6519m41p31-1822m47e38-1026m59e38-3127m48e32-1528m47e38-1533cMWG70236m48e41-0343HVBKASI49m48e32-0152m48e32-1354BMag37864m41p31-0881MWG503a87m33p31-1889m41p31-0792S-12098m59e36-08108m59e36-07116S-217118m31p31-17124MWG82126MWG46130
m59e36-180m32p31-033cMWG6914MWG63b10m33p31-0518m59e38-0833m59e36-0434S-reg1937MWG58439
m48e38-1558m48e38-0159m47e32-2062m59e38-2964cMWG68070m48e38-1674m47e32-1880BMag22592MWG555b100
m41p31-14119
MWG961133BMag13147m59e41-15153m59e41-16154S-L8156m41p31-11159m31p31-15168m41p31-09170m59e38-26183
m41p31-01193
3Hm31p31-060m48e38-115m59e38-0112m59e36-0113m47e38-0318m33p31-0422m33p31-1523MWG7724MWG203329WHS17841m32p31-1653MWG5857BMac18163m48e38-1270m47e38-1176m48e38-0779m47e32-0285m48e32-2187m48e32-0590m48e32-1092m47e41-0499MWG57103m47e41-10110m48e32-17128
m48e38-18144m47e38-23146WMS6150m33p31-12159HVM67162
4Hm32p31-010MWG5302BMag2066m48e32-1110MWG56420m42p31-0722m33p31-1424MWG4a36MWG1843MWG83651m59e36-0962MWG3965m42p31-0375MWG62676m48e38-0679m47e32-0881BMag32182MWG649a83m48e32-2285m47e41-0788m47e32-0499m31p31-24107MWG53109m41p31-20114m42p31-01121cMWG633127MWG539135m32p31-06136m41p31-19141S-9203151
7H
qml1qml2
qml3
qml4qml5
P < 0.0005
Mla
MlLa
Mlf
Leaf rust, powdery mildew and scald resistance genes on the barley chromosomes
1H 2H 3H 4H 6H 7H
RhyRh
qRh2
qRh3
Rrs13
qRh4Rh2
qRh8
5H
Rph4
[Rph1]
qLr2
qLr1
qLr3
[Rph7]
[Rph11]
qLr4
qLr9
Rph2RphQ
Rph9Rph12
qLr5qLr6
qLr7
MlaMlk
Mlnn
MlGa
qPm1qPm2
MlLa
qPm3
Mlg
mlo
qPm4
qPm5
MlhqPm8
qPm9
mlt
Mlf
qPm10
qPm11
qPm12
qPm6qPm7
Mlj
Malting qualty traits detected in wild barley
Trait a
QT
L in S42IL
Chr. b
Introgression (in cM) c
Bin range
d
Introgression line
Effect e
LSM
EA
NS
(IL) f
Diff. g
RP
[S42IL] (%
) h
QT
L in parent population S
42i
Candidate genes
j
AA
QAa.S42IL-4H.a 4H 14–31 2–4 S42IL-116 L + I 546.9 91.3 20 QAa.S42-4H.a
QAa.S42IL-4H.b 4H 170–190 12–13 S42IL-124 L + I 529.4 73.9 16 .2 QAa.S42-4H.c Bmy1 1
GSF
QGsf.S42IL-2H.a 2H 17–42 2–4 S42IL-107 L + I 86.4 6.7 8.4 Ppd-H1 6
QGsf.S42IL-2H.c 2H 80–86 7–8 S42IL-110 L 85 5.3 6.7
QGsf.S42IL-4H.a 4H 31–95 4–8 S42IL-119 L 84.6 4.9 6.2
KOL QKol.S42IL-4H.a 4H 14–31 2–4 S42IL-116 L 46.4 2.2 5.1
Schmalenbach and Pillen, 2009
QTL detected for agronomic traits in wild barley
TraitaQTL in S42IL
Chr.bIntrogression (in cM)c
Introgression line
EffectdLSMEANS (IL)e Diff.f RP (IL) %g
EARQEar.S42IL-6H.a 6H 145–155 S42IL-132 I 1359.0D07 391 40.4
HEAQHea.S42IL-1H.a 1H 0–85 S42IL-102 L + I 68.2 1.9 2.9
HEIQHei.S42IL-1H.a 1H 70–85 S42IL-105 L + I 89.9 9.1 11.3
LAHQLah.S42IL-1H.a 1H 70–85 S42IL-105 I 6.3G07 3.2 100
YLDQYld.S42IL-4H.a 4H 31–57 S42IL-118 I 73.5D07 7.8 11.8
Schmalenbach et al. 2008
No. Favorable 1) Max. RP 2) Difference Trait QTLs exot. alleles [Hsp:Hv] [Hsp]–[Hv]
Heading 10 8 - 7.9 % - 5.7 d
Yield 12 3 + 7.1 % + 4.0 dt/ha
Powdery 8 6 - 51.5 % 2.5 scoremildew
1) BC2DH lines with the exotic genotype [Hsp] are on a verage superior to lines with the elite barley genotype [Hv] at a given marker locus.
2) Performance of homozygous exotic genotypes relative to homozygous elite barley genotypes at a marker locus.
Localization of QTL regions and favorableexotic QTL alleles in S42
Pillen et al.
Summary of QTL verification with 39 S42-ILs
- Hsp allele reduces trait+ Hsp allele increases trait
= QTL verifiedPillen et al.
0
1420
28
39
5263
6870
75
85
105115
130
1H
144
65
162
30
25
49
65
94
100
110
130
155
165
175
190
3H
70
142125
31
44
57
80
95
125
130131132
150
170
180
190
4H
55
17
27
92
107
122
139
143
146
159
2H
42
8680
67
0-14
14-3
9
39-7
070
-85
52-7
0
17-9
2
17-4
2
17-2
767
-92
80-8
6
65-7
0
155 -
190
155 -
175
155 -
165
100 -
130
14-4
4
31-5
7
80-9
5
31-9
5
14-2
517
0 -19
0
125 -
132
125 -
170
55-5
7
20
67
190
4480
95
5H0
12
24
43
48
69
85
126
137
162
165
43-4
816
2 -16
569
-85
69
6
40
96
103
107
112
135
145
155
6H
40-1
12
96-1
12
112 -
155
135 -
155
145 -
155
640
9611
2
19
27
50
62
75
9394
100
120
133
146152155
166
178180181
7H
5062
-75
75-1
55
146 -
166
146 -
155
166 -
181
178 -
181
133
Regions of chromosomes so far without ILsFinished IL Finished Sub- IL
(Schmalenbach et al. , unpublished)Days to heading
*) favorable exotic allele
HEA
ILs selected for heading date QTLs in GABI-Malt
HEA*
HEA*
HEA
HEA*
HEA*
HEA*
Conclusion
• Wild barley has been shown to posses new and effective sources of disease resistance
• Transfer of resistance gene from wild barley to cultivated barley has been achieved successfully
• QTL for yield and malting quality have been detected in wild barley.
• Future will show whether these QTLs could be transferred successfully in the cultivars