seramir p1 2011 - system biosciences...pcr+ivt amplify m un amp 100 bp 200 bp amplified exorna 500...

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• Exosomes are released from tumors in high abundance into the circulation • Exosome cargo reflects the origin and physiological state of the source cells • RNAs and MicroRNAs are found in high abundance in circulating exosomes • Precipitate exosomes from patient biofluids easily with ExoQuick • Purify exoRNAs with SeraMir columns • Tail and tag all exoRNAs for qPCR, cDNA amplification and T7 IVT • Amplify exoRNAs for Microarrays and NextGen Sequencing microRNA Research Highlights SeraMir Exosome RNA Amplification Purify and amplify serum exosomal RNAs for robust microRNA and noncoding RNA biomarker discoveries RNAs present in patient body fluids are a rich and untapped source of disease-related biomarkers. The RNAs are stable in serum because they are encapsulated in circulating exosomes. Exosomes are 40–100 nm membrane vesicles secreted by most cell types in vivo and in vitro. Exosomes are found in blood, urine, amniotic fluid, malignant ascite fluids and contain distinct subsets of microRNAs depending upon the tumor or tissue from which they are secreted. The SeraMir kit includes everything needed to accurately and sensitively measure RNAs from serum samples. Exosomes are efficiently isolated using SBI’s ExoQuick solution, and the exoRNAs are purified using a phenol-free lysis buffer and rapid spin columns. The SeraMir kit enables the 3‘ tailing and simultaneous tagging of both 5‘ and 3’ ends during cDNA synthesis - ready for qPCR. Primers for PCR amplification are included for highly sensitive applications. Isolate exosomes in one step - Easily purify the Exosome RNAs www.systembio.com/seramir Accelerating Discoveries in Functional Genomics System Biosciences Supernatant Exosome pellet Tail and Tag exoRNA Serum sample EM image PCR+IVT Amplify M Un Amp 100 bp 200 bp Amplified exoRNA 500 bp ExoQuick™ exosome precipitation · Reduce variability - isolate exosomes first with ExoQuick · Increase sensitivity - amplify exoRNAs for qPCR · Gain more data - use T7 IVT amplified sense exoRNAs for microarrays and NextGen sequencing Exosome RNA Amplification SeraMir

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Page 1: SeraMir p1 2011 - System Biosciences...PCR+IVT Amplify M Un Amp 100 bp 200 bp Amplified exoRNA 500 bp ExoQuick exosome precipitation · Reduce variability - isolate exosomes first

• Exosomes are released from tumors in high abundance into the circulation

• Exosome cargo reflects the origin and physiological state of the source cells

• RNAs and MicroRNAs are found in high abundance in circulating exosomes

• Precipitate exosomes from patient biofluids easily with ExoQuick

• Purify exoRNAs with SeraMir columns

• Tail and tag all exoRNAs for qPCR, cDNA amplification and T7 IVT

• Amplify exoRNAs for Microarrays and NextGen Sequencing

microRNA Research

Highlights

SeraMir™ Exosome RNA AmplificationPurify and amplify serum exosomal RNAs for robustmicroRNA and noncoding RNA biomarker discoveries

RNAs present in patient body fluids are a rich and untapped source of disease-related biomarkers. The RNAs are stable in serum because they are encapsulated in circulating exosomes. Exosomes are 40–100 nm membrane vesicles secreted by most cell types in vivo and in vitro. Exosomes are found in blood, urine, amniotic fluid, malignant ascite fluids and contain distinct subsets of microRNAs depending upon the tumor or tissue from which they are secreted. The SeraMir kit includes everything needed to accurately and sensitively measure RNAs from serum samples. Exosomes are efficiently isolated using SBI’s ExoQuick solution, and the exoRNAs are purified using a phenol-free lysis buffer and rapid spin columns. The SeraMir kit enables the 3‘ tailing and simultaneous tagging of both 5‘ and 3’ ends during cDNA synthesis - ready for qPCR. Primers for PCR amplification are included for highly sensitive applications.

Isolate exosomes in one step - Easily purify the Exosome RNAs

www.systembio.com/seramir

Accelerating Discoveries in Functional GenomicsSystem Biosciences

Supernatant

Exosome pellet

Tail and Tag exoRNA

Serum sample

EM image

PCR+IVT Amplify

M Un Amp

100 bp

200 bp

AmplifiedexoRNA

500 bp

ExoQuick™exosomeprecipitation

· Reduce variability - isolate exosomes first with ExoQuick

· Increase sensitivity - amplify exoRNAs for qPCR

· Gain more data - use T7 IVT amplified sense exoRNAs for microarrays and NextGen sequencing

Exosome RNA AmplificationSeraMir

Page 2: SeraMir p1 2011 - System Biosciences...PCR+IVT Amplify M Un Amp 100 bp 200 bp Amplified exoRNA 500 bp ExoQuick exosome precipitation · Reduce variability - isolate exosomes first

SeraMir Exosome RNA Amplification Kit ™

© 2011 System Biosciences, Inc. All rights reserved. System Biosciences and the System Biosciences logo are trademarks of System Biosciences, Inc.

System Biosciences, Inc.265 North Whisman Rd.Mountain View, CA 94043

Toll Free: 888.266.5066Fax: 650-968-2277Email: [email protected]

How the Kit Works

time making discoveries. To learn more, visit our website at www.systembio.com/service or call us at 888-266-5066.

Tail and Tag exoRNA

+

Serum

ExoQuickSolution

Simple one-stepprecipitation

Supernatant

Exosome pellet

Exosome Lysate

BINDWASHELUTE

SPIN

Phenol-freeExosome Lysis

Tag both ends of exoRNAs. Built-in T7 promoter at 5’ endfor robust IVT amplification.

Phenol/TrizolMethods

SeraMirTechnology

Perform qPCR profiling on cDNA or use T7 IVT of exoRNAs for Arrays and NGS

Serum RNA prepared by conventional Trizol versus the SeraMir kit. Profiling of 380 Human microRNAs.

We Also Offer Custom Services

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4 5’ adaptorprimer

5’ 3’

5’3’3’5’

Polyadenylate

5’ 3’

3’ adaptorprimer

Exosomal RNA

AAAAAAAAAA

RTreaction

T7

E xo s o m e R N A P r o fi l i n gSeraMir

3 minuteexoRNAclean-up

M Un Amp

100 bp

500 bp

1500 bp

Exosomal

RNA cDNA

PCR Amplify PCR+IVT AmplifyM Un Amp

100 bp

200 bp

AmplifiedexoRNA

500 bp

5’ SwitchAdaptor