seramir p1 2011 - system biosciences...pcr+ivt amplify m un amp 100 bp 200 bp amplified exorna 500...
TRANSCRIPT
• Exosomes are released from tumors in high abundance into the circulation
• Exosome cargo reflects the origin and physiological state of the source cells
• RNAs and MicroRNAs are found in high abundance in circulating exosomes
• Precipitate exosomes from patient biofluids easily with ExoQuick
• Purify exoRNAs with SeraMir columns
• Tail and tag all exoRNAs for qPCR, cDNA amplification and T7 IVT
• Amplify exoRNAs for Microarrays and NextGen Sequencing
microRNA Research
Highlights
SeraMir™ Exosome RNA AmplificationPurify and amplify serum exosomal RNAs for robustmicroRNA and noncoding RNA biomarker discoveries
RNAs present in patient body fluids are a rich and untapped source of disease-related biomarkers. The RNAs are stable in serum because they are encapsulated in circulating exosomes. Exosomes are 40–100 nm membrane vesicles secreted by most cell types in vivo and in vitro. Exosomes are found in blood, urine, amniotic fluid, malignant ascite fluids and contain distinct subsets of microRNAs depending upon the tumor or tissue from which they are secreted. The SeraMir kit includes everything needed to accurately and sensitively measure RNAs from serum samples. Exosomes are efficiently isolated using SBI’s ExoQuick solution, and the exoRNAs are purified using a phenol-free lysis buffer and rapid spin columns. The SeraMir kit enables the 3‘ tailing and simultaneous tagging of both 5‘ and 3’ ends during cDNA synthesis - ready for qPCR. Primers for PCR amplification are included for highly sensitive applications.
Isolate exosomes in one step - Easily purify the Exosome RNAs
www.systembio.com/seramir
Accelerating Discoveries in Functional GenomicsSystem Biosciences
Supernatant
Exosome pellet
Tail and Tag exoRNA
Serum sample
EM image
PCR+IVT Amplify
M Un Amp
100 bp
200 bp
AmplifiedexoRNA
500 bp
ExoQuick™exosomeprecipitation
· Reduce variability - isolate exosomes first with ExoQuick
· Increase sensitivity - amplify exoRNAs for qPCR
· Gain more data - use T7 IVT amplified sense exoRNAs for microarrays and NextGen sequencing
Exosome RNA AmplificationSeraMir
SeraMir Exosome RNA Amplification Kit ™
© 2011 System Biosciences, Inc. All rights reserved. System Biosciences and the System Biosciences logo are trademarks of System Biosciences, Inc.
System Biosciences, Inc.265 North Whisman Rd.Mountain View, CA 94043
Toll Free: 888.266.5066Fax: 650-968-2277Email: [email protected]
How the Kit Works
time making discoveries. To learn more, visit our website at www.systembio.com/service or call us at 888-266-5066.
Tail and Tag exoRNA
+
Serum
ExoQuickSolution
Simple one-stepprecipitation
Supernatant
Exosome pellet
Exosome Lysate
BINDWASHELUTE
SPIN
Phenol-freeExosome Lysis
Tag both ends of exoRNAs. Built-in T7 promoter at 5’ endfor robust IVT amplification.
Phenol/TrizolMethods
SeraMirTechnology
Perform qPCR profiling on cDNA or use T7 IVT of exoRNAs for Arrays and NGS
Serum RNA prepared by conventional Trizol versus the SeraMir kit. Profiling of 380 Human microRNAs.
We Also Offer Custom Services
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4 5’ adaptorprimer
5’ 3’
5’3’3’5’
Polyadenylate
5’ 3’
3’ adaptorprimer
Exosomal RNA
AAAAAAAAAA
RTreaction
T7
E xo s o m e R N A P r o fi l i n gSeraMir
3 minuteexoRNAclean-up
M Un Amp
100 bp
500 bp
1500 bp
Exosomal
RNA cDNA
PCR Amplify PCR+IVT AmplifyM Un Amp
100 bp
200 bp
AmplifiedexoRNA
500 bp
5’ SwitchAdaptor