Howells, Richard D.

July 8, 2015

CURRICULUM VITAE

NAME: Richard Donald Howells, Ph.D.

HOME ADDRESS: 34 Rickland DriveRandolph, NJ 07869

OFFICE ADDRESS: UMDNJ-New Jersey Medical SchoolBiochemistry and Molecular Biology 185 South Orange AvenueUniversity HeightsNewark, NJ 07103-2714

973-972-5652 (Office)973-972-4772 (Laboratory)973-972-5594 (FAX)howells@umdnj.edu (email)

1. EDUCATION

a. Undergraduate

1973-1976 B.A., BiochemistryRutgers-The State University of New JerseyNew Brunswick, NJ

b. Graduate and Professional

1976-1978 Graduate Studies in BiochemistryRutgers University

1978-1982 Graduate Studies in Microbiology, PharmacologyPh.D., PharmacologyNew York UniversityNew York, NYThesis Advisor: Dr. Eric J. Simon

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Howells, Richard D.

2. POSTDOCTORAL TRAINING

a. Internships and Residencies -

b. Research Fellowships

1982-1984 Postdoctoral FellowLaboratory of Molecular NeurobiologyRoche Institute of Molecular BiologyNutley, NJ 07110Supervisor: Dr. Sidney Udenfriend

3. MILITARY -- None

4. LICENSURE

a. N. J. Medical License -- N/A

b. Other State Medical Licenses -- N/A

5. CERTIFICATION

a. Specialty Board -- N/A

b. Subspecialty Board -- N/A

6. NARCOTICS CERTIFICATION

a. N. J. CDS

Researcher R000654 - expiration date: 3/31/16

b. U.S. DEA

Researcher RHO 223040 - expiration date: 10/31/15

7. UNIVERSITY APPOINTMENTS

1986-1988 Adjunct Assistant Professor2

Howells, Richard D.

Laboratory of Neurobiology and BehaviorThe Rockefeller UniversityNew York, NY 10021

9/1987-6/1994 Assistant ProfessorDepartment of Biochemistry and Molecular BiologyUMDNJ-New Jersey Medical SchoolNewark, NJ 07103

7/1994-6/2003 Associate ProfessorDepartment of Biochemistry and Molecular BiologyUMDNJ-New Jersey Medical SchoolNewark, NJ 07103

12/1998-present Associate ProfessorDepartment of Neurosciences (joint appointment)UMDNJ-New Jersey Medical SchoolNewark, NJ 07103

7/2003-6/2013 ProfessorDepartment of Biochemistry and Molecular BiologyUMDNJ-New Jersey Medical SchoolNewark, NJ 07103

8/2003-9/2011 Assistant Dean for the M.D./Ph.D. Program at New Jersey Medical School

7/2011-6/2013 ProfessorMolecular Biology, Genetics and Cancer TrackUMDNJ-Graduate School of Biomedical SciencesNewark, NJ 07103

7/2011-6/2013 ProfessorCell Biology, Neuroscience and Physiology TrackUMDNJ-Graduate School of Biomedical SciencesNewark, NJ 07103

7/2013-present ProfessorDepartment of Biochemistry and Molecular BiologyRutgers-New Jersey Medical School

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Howells, Richard D.

Newark, NJ 07103

7/2013-present ProfessorMolecular Biology, Genetics and Cancer TrackRutgers-Graduate School of Biomedical SciencesNewark, NJ 07103

7/2013-present ProfessorCell Biology, Neuroscience and Physiology TrackRutgers-Graduate School of Biomedical SciencesNewark, NJ 07103

7/2014-present ProfessorDepartment of Microbiology, Biochemistry and Molecular GeneticsRutgers-New Jersey Medical SchoolNewark, NJ 07103

8. HOSPITAL APPOINTMENTS -- N/A

9. OTHER PROFESSIONAL POSITIONS AND MAJOR VISITING APPOINTMENTS

1978-1981 Graduate Research Assistant Department of PharmacologyNew York University Medical CenterNew York, NY 10016

1984-1985 Research AssociateLaboratory of Molecular NeurobiologyRoche Institute of Molecular BiologyNutley, NJ 07110Supervisor: Dr. Sidney Udenfriend

1985-1987 Research FellowLaboratory of Molecular NeurobiologyRoche Institute of Molecular BiologyNutley, NJ 07110Supervisor: Dr. Sidney Udenfriend

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Howells, Richard D.

10. AWARDS AND HONORS

1992 Selected for Special Review Committee (SRCD-26), National Institute on Drug Abuse

1994 Served as reviewer for United States-Israel Binational Science Foundation

1995 Chosen as Faculty Judge for Graduate Student Association Research in Excellence Awards

1996 Member of Advisory Committee on Personnel for Research B, American Cancer Society

Served as grant reviewer for Israel Science FoundationRecipient of International Narcotic Research Conference travel

awardServed as grant reviewer for City University of New York

Research Award Program

1997 Served as grant reviewer for United States-Israel Binational Science Foundation

Served on National Institute on Drug Abuse Training and Career Development Special Emphasis Panel

1998 Served as reviewer for Arkansas Science and Technology Authority Basic Research Grant Program

Served as reviewer for U.S.-Israel Binational Science FoundationRecipient of International Narcotic Research Conference travel

awardScientific consultant for Weitzman & Weitzman, Counselors at

Law, Maplewood, NJ

1999 Chosen as Faculty Judge for Graduate Student Association Research in Excellence Awards

Chosen as panelist on use of DNA chip arrays by Affymetrix

2000 Selected as grant reviewer for National Science FoundationRecipient of Research Recognition Award, UMDNJ

2001 Recipient of nomination for the Golden Apple Teaching Award-UMDNJ-NJMS

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Howells, Richard D.

Served as invited reviewer of new National Institute on Drug Abuse (NIDA) Cutting-Edge Basic Research Awards (CEBRA) program

2002 Recipient of Certificate of Appreciation from the Director of the National Institute on Drug Abuse, Glen Hanson, PhD, DDS, for dedicated service to NIDA as a founding member of the Training and Career Development Subcommittee

2003 Nominated for Master Educator Award, UMDNJ-NJMS

2004 Thesis mentor and nominator of Keith Christoffers, recipient of the Faculty Organization Student of the Year Award

2005 Successfully nominated Matt Hosler, MD/PhD, for the Stanley Bergen, MD, Award

2008 Successfully nominated Ken Wannemacher, graduate student, for the Joseph Fu Memorial Scholarship

2008 Successfully nominated Alexandra Terskiy, MD/PhD student, for the Dean Morris Schaefer Award

2008 Successfully nominated Rivka Stone, MD/PhD student, as an Executive Women of NJ Scholar

2009 Successfully nominated Alexandra Terskiy, MD/PhD student, for the Philanthropic Educational Organization Scholar Award

2010 Successfully nominated Rivka Stone, MD/PhD student, for the Philanthropic Educational Organization Scholar Award

2011 Successfully nominated Rivka Stone, MD/PhD student, for Stanley Bergen Award

2011 Successfully nominated Rivka Stone, MD/PhD student, as an Executive Women of NJ Scholar

2012 Received NJMS Faculty Award, Twenty Five Years of Dedicated Distinguished Service to New Jersey Medical School

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Howells, Richard D.

2013 Received the 2013 Foundation of UMDNJ Excellence in Research Award

11. BOARDS OF DIRECTORS/TRUSTEES

2004 – present Member of the Irene and Eric Simon Brain Research Foundation Advisory Committee

12. MAJOR COMMITTEE ASSIGNMENTS

a. National and Regional

1996 Member of Advisory Committee on Personnel for Research B, American Cancer Society

1997 Member of NIH/NIDA Training and Career Development Special Emphasis Panel

1998 Member of NIH/NIDA Special Emphasis Panel ZDA1-MXS-MO1Member of NIH/NIDA Special Emphasis Panel ZDA1-MXS-M21Member of NIH/NIDA Special Emphasis Panel ZDA1-MXS-M09Member of NIH/NIDA Special Emphasis Panel ZDA1-MXS-M30Member of NIH/NIDA Special Emphasis Panel ZDA1-MXS-M12Member of NIH/NIDA Special Emphasis Panel ZDA1-MXS-M33

1999 Member of NIH/NIDA Special Emphasis Panel ZDA1-MXS-M20Member of NIH/NIDA Training and Career Development Study

Section (NIDA-K)Member of NIH/NIDA Special Emphasis Panel ZDA1 KXA-N (03)

2000 Member of NIH/NIDA Training and Career Development Study Section (NIDA-K)

Member of NIH/NIDA-K Conflicts Special Emphasis Panel ZDA1 RXL E-07-S

2001 Member of NIH/NIDA Training and Career Development Study Section (NIDA-K)

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Howells, Richard D.

Member of NIH/NIDA Cutting-Edge Basic Research Award (CEBRA) Review Committee ZDA1 TXL-Q10

2002 Member of NIH/NIDA Training and Career Development Study Section (NIDA-K)

Member of NIH/NIDA-K Special Emphasis Panel ZDA1 KXA-N(16)

Member of NIH/NIDA-K Special Emphasis Panel ZDA1 KXA-N(17)

Member of NIH/NIDA-K Special Emphasis Panel ZDA1 KXA-N(19)

2003 Member of NIH/NIDA-K Training and Career Development Subcommittee

2004 Member of NIH/NIDA-K Training and Career Development Subcommittee

2008 Co-chaired the GPCR II Symposium, Society for Neuroscience Annual Meeting, Washington DC November 2008

b. Medical School

1988-1989 Ad Hoc Committee for Student Admissions

1988-present Participant in annual NJMS Convocation Ceremony

1989 LCME accreditation participant

1990 Search Committee: Chairperson of Pediatrics

1990-1992 Institutional Animal Care and Use Committee

1993-1996 Academic Programs and Policies Committee

1994-1995 Faculty Committee on Appointments and Promotions

1995-1997 Faculty Council

1997-2011 M.D./Ph.D. Program Committee8

Howells, Richard D.

1998-2001 Search Committee: Chairperson of Pediatrics

1999-2003 Founding Course Director for Molecular and Cellular Biology, Core Curriculum, Graduate School of Biomedical Sciences

1999-present Student Advisor for medical students

2000 Department of Pediatrics Internal Review Committee

2000-present DMD/PhD Advisory CommitteeMember of Biomedical Research Support Committee

2003-2005 Faculty CouncilInterviewer for Chair, Microbiology and Molecular Genetics

2003-2011 Chairman, MD/PhD Program Committee

Executive Council, Graduate School of Biomedical Science

2004-2005 Liaison Committee on Medical Education (LCME) Self-Study Taskforce: Medical Students

Admissions Subcommittee, LCME Taskforce

2005 Liaison Committee on Medical Education, Site Visit Participant

2005 Middle States Accreditation Site Visit Participant

2005-2010 Search Committee: Chairperson of Neurology and Neuroscience

2006-2007 HHMI Quantitative Neuroscience Organizing Committee

2007-2008 HHMI Quantitative Neuroscience Faculty Search Committee

2007-present Member, Grant Review Committee for FUMDNJ awards, Office of Research and Sponsored Programs

2009 Reviewer for the NIH Challenge Award applications in the ZRG1 MDCN-A (58)R meeting

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Howells, Richard D.

2014-present Elected member to the Rutgers Biomedical and Health Sciences Faculty Council

2014-present Member of the Basic & Clinical Science Integration Working Group, Rutgers- School of Dental Medicine

2014-present Member of the Course Directors Committee, Rutgers- School of Dental Medicine

2015-present Member of the Executive Council, Rutgers- School of Dental Medicine

c. Hospital--N/A

d. Department

1989 Dental Biochemistry Curriculum Committee

1990-1992,1994-1999 Coordinator, Departmental Seminar Series

1995-2002 Graduate Student Committee

1998 Faculty Search Committee

2001 Departmental Subcommittee on Ph.D. Course RequirementsFaculty Search Committee

2002 Faculty Search CommitteeDepartmental Self Study Committee, Medical Biochemistry

Subcommittee

2004 Recitation Subcommittee, Molecular and Genetic Medicine Curriculum Committee

2008 Graduate Program Subcommittee

2009-present Interviewer for Graduate Student Recruitment Day (BMB, Neuroscience, MBGC track, CBNP track

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Howells, Richard D.

Doctoral Thesis Committees

1989 Ms. Yu-Chu Huang - Biochemistry and Molecular Biology

1990 Dr. Anthony Iacopino - Biochemistry and Molecular Biology

1991 Ms. Jenfen Liu - Oral Biology

1992 Ms. Theresa Matkovits - Biochemistry and Molecular Biology Mr. Amartya Basu - Biochemistry and Molecular BiologyMr. Zhi-Fang Chou - Microbiology and Molecular Genetics

1994 Mr. Keith Rosenbach - Microbiology and Molecular GeneticsMr. Zuwei Qian - Microbiology and Molecular GeneticsMr. Juan Herrero - Microbiology and Molecular Genetics

1995 Mr. Kajal Chowdhury - Biochemistry and Molecular Biology

1996 Mr. Ming Zhang - Neuroscience, Rutgers-NewarkMr. Wenwu Gao - Microbiology and Molecular GeneticsMs. Diane Kozireski - NeuroscienceMr. Huicong Cai - Biochemistry and Molecular BiologyMr. Nan Zhang - Pharmacology and Physiology

1997 Ms. Rong Shu - Physiology, Pharmacology and ToxicologyMr. Kajal Chowdhury - Biochemistry and Molecular BiologyMr. Wen Yang - Biochemistry and Molecular BiologyMr. Weikang Tao - Biochemistry and Molecular BiologyMr. Syed Reza - Biochemistry and Molecular BiologyMr. Peter Alfinito – Neuroscience

1998 Ms. Yingxia Liu - NeuroscienceMs. Jin Jin - Biochemistry and Molecular BiologyMr. Steve Tuske- Biochemistry and Molecular BiologyMr. Frank Barletta- Biochemistry and Molecular Biology

1999 Ms. Cristine Woititz-Pathology and Laboratory MedicineMs. Kathy Piparo- Biochemistry and Molecular Biology

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Howells, Richard D.

2000 Mr. Basil Hantash- Pharmacology and PhysiologyMr. Bin Tian- Biochemistry and Molecular Biology

2002 Ms. Grace Pinhal-Enfield- Anatomy and Molecular Medicine

2003 Ms. Lorena Maria Equez - Microbiology and Molecular Genetics

2004 Mr. Matthew Hosler- MD/PhD ProgramMr. Elie Needle- Biochemistry and Molecular BiologyMs. Ligia Westrich- Neuroscience

2005 Ms. Olga Chernysh- Pharmacology and PhysiologyMr. Joseph Quispe- Cell Biology and Molecular Medicine

2006 Mr. Luis Muniz- Biochemistry and Molecular BiologyMs. Qi Wang- Biochemistry and Molecular Biology

2007 Mr. Walid Abushahba- Biochemistry and Molecular BiologyMr. Baskar Balan- Biochemistry and Molecular Biology

2009 Ms. Anna Angelova- Pharmacology and Physiology

2010 Ms. Wenting Luo- Biochemistry and Molecular BiologyMs. Victoria Prince- MD/PhD ProgramMr. Kevin Christian Donahue- MD/PhD Program

2012 Mr. Raghavendra Shamanna- Biochemistry and Molecular BiologyMs. Danielle Scarola- Neuroscience (Master’s thesis committee)

Qualifying Examination Committees

1989 Ms. X. Y. Cai - Microbiology and Molecular GeneticsMr. Louis Scala - Biochemistry and Molecular BiologyMs. Jun Yin - Biochemistry and Molecular Biology

1990 Mr. Simi Vincent - PharmacologyMs. Kristen Gustafson - AnatomyMs. Jenfen Liu - Oral BiologyMs. Sarah S. Kolla - Laboratory Medicine and PathologyMr. Atul Deshpande - Microbiology and Molecular Genetics

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Howells, Richard D.

1991 Mr. Debaprasad Saha- Biochemistry and Molecular Biology

1992 Mr. Huicong Cai- Biochemistry and Molecular BiologyMr. William Wang- Biochemistry and Molecular Biology

1993 Ms. Mandana Shahrestanifar- Biochemistry and Molecular Biology

1995 Ms. Carolyn Harmon - Laboratory Medicine and PathologyMr. Ming Zhang - Laboratory Medicine and Pathology

1996 Ms. Jin Jin - Biochemistry and Molecular BiologyMs. Karen Sooy - Biochemistry and Molecular BiologyMr. Christopher Schaefer - Microbiology and Molecular GeneticsMr. Peter Alfinito - NeuroscienceMr. Lan Jiang - Physiology and Pharmacology and Toxicology

1997 Mr. Eric White - Microbiology and Molecular Genetics

1998 Mr. Min Gao - Microbiology and Molecular GeneticsMs. Leena Bagle - Biochemistry and Molecular BiologyMr. Michael Huening - Biochemistry and Molecular BiologyMr. Frank Barletta - Biochemistry and Molecular BiologyMr. Keith Christoffers - NeuroscienceMs. Xie Xin – Neuroscience

1999 Mr. Kwaben Opuni-Department of Pharmacology and PhysiologyMs. Lorena Maria Equez - Microbiology and Molecular GeneticsMr. Basil Hantash – MD/PhD ProgramMr. Elie Needle- Biochemistry and Molecular Biology

2000 Ms. Ling Kang- NeuroscienceMr. Vamsi Gollapalli-Neuroscience

2001 Mr. William Simmons- Laboratory Medicine and PathologyMr. Li Cheng-Microbiology and Molecular GeneticsMs. Yifan Song-Pharmacology and Physiology

2002 Dr. Wafaa El-Sayed- Pharmacology and PhysiologyMs. Judith N. Gorski- Neuroscience

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Howells, Richard D.

Mr. Wei Bu- Microbiology and Molecular GeneticsMs. Irina Sigal- MD/Ph.D. programMr. Luis Muniz- Biochemistry and Molecular Biology

2004 Mr. Jay Turner- MD/PhD ProgramMr. Nicola Pierbon- Pharmacology and PhysiologyMs. Jiaying (Joyce) Huang- Biochemistry and Molecular BiologyMr. Walson Metzger- MD/PhD ProgramMr. Shakti Ramkissoon- MD/PhD Program

2005 Ms. Olga Chernysh- Pharmacology and PhysiologyMr. Nazish Sayed- Pharmacology and Physiology

2006 Mr. Walid Abushahba- Biochemistry and Molecular BiologyMr. Baskar Balan- Biochemistry and Molecular Biology

2007 Mr. Reza Razavi- Interdisciplinary ProgramMs. Jennifer Woodbury- MD/PhD ProgramMs. Mahrukh M. Bandey- Microbiology and Molecular GeneticsMs. Nicole Pannucci- MD/PhD ProgramMs. Amrita Ghosh- MD/PhD Program

2008 Ms. Alexandra Terskiy- MD/PhD ProgramMs. Anna Angelova- Pharmacology and Physiology

2009 Ms. Khan Nguyen- Biochemistry and Molecular Biology

2010 Ms. Jyoti Joshi- Interdisciplinary ProgramMs. Wenting Luo- Biochemistry and Molecular BiologyMr. Thomas Comollo- Biochemistry and Molecular BiologyMs. Smita Shukla- Cell Biology and Molecular Medicine

2011 Ms. Mingyue Han- Cell Biology and Molecular Medicine

2012 Ms. Tanya Seth- Biochemistry and Molecular Biology

2013 Ms. Shravanthi Chidambaram- Molecular Biology, Genetics and Cancer Track

e. Editorial Boards14

Howells, Richard D.

Current Molecular Pharmacology

AdHoc Reviewer for the following:

Archives of Biochemistry and BiophysicsBlackwell Science, Inc.Brain Research Cancer ResearchDevelopmental Brain ResearchEuropean Journal of NeuroscienceJournal of Biological ChemistryJournal of ImmunologyJournal of Medicinal ChemistryJournal of Molecular SignalingJournal of Natural Products- 2012Journal of NeurochemistryJournal of Pharmacology and Experimental TherapeuticsLife SciencesMolecular Brain ResearchMolecular Cell Biology, Lodish et al., Freeman Pub.Molecular PharmacologyNational Science FoundationNeurochemical ResearchNeuroendocrinologyNIDA Drug Supply ProgramProceedings of the National Academy of SciencesRegulatory PeptidesBrain Research Bulletin

13. MEMBERSHIPS, OFFICES AND COMMITTEE ASSIGNMENTS IN PROFESSIONAL SOCIETIES

1977-present American Association for the Advancement of Science1978-present International Narcotic Research Conference1986-present Endocrine Society1986-present Society for Neuroscience1987-present New York Academy of Sciences1988-present Sigma Xi1991-present American Society of Biochemistry and Molecular Biology

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Howells, Richard D.

1998-present American Society of Pharmacology and Experimental Therapeutics

1998-present Congressional Liaison Committee-ASPET2007-present American Association for Cancer Research

14. MAJOR RESEARCH INTERESTS

Regulation of eukaryotic gene expression in the nervous system. Molecular basis of tolerance and physical dependence resulting from chronic opiate drug use. Molecular cloning of G protein-coupled receptors. Solubilization and purification of G protein-coupled receptors. Structure/function analysis of opioid receptors. Signal transduction pathways activated by opioids. Opioid receptor turnover, agonist-induced receptor down regulation, antagonist-induced receptor up-regulation. Ubiquitination and targets of the proteasome complex. Analysis of receptor post-translational modifications and interacting proteins by mass spectrometry. Select opioids ligands as pharmacological chaperones that stabilize and induce proper receptor folding during synthesis in the secretory pathway. Naltrindole and structural analogs as anti-cancer therapeutic agents.

15. GRANT HISTORY

a. Principal Investigator

Title: The Regulation of Opioid Peptide Gene Expression in TestisAgency: The Foundation of UMDNJDate: July 1, 1988-June 30, 1989Award: $25,000

Title: Molecular Consequences of Tolerance and DependenceAgency: Biomedical Research Support Grant (BRSG)Date: July 18, 1988-July 18, 1989Award: $9,998

Title: Molecular Consequences of Tolerance and DependenceAgency: National Institute on Drug Abuse, DA05819Date: July 1989-June 1993Award: $476,535

Title: Equipment Grant: Departmental Autoclave SterilizerAgency: Biomedical Research Support GrantDate: January 11, 1990

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Howells, Richard D.

Award: $10,000

Title: Small Instrumentation AwardAgency: Alcohol, Drug Abuse and Mental Health Administration Date: October 1990 Award: $8,736

Title: Small Instrumentation AwardAgency: Alcohol, Drug Abuse and Mental Health Administration Date: November 1991Award: $5,000

Title: Small Instrumentation AwardAgency: Alcohol, Drug Abuse and Mental Health Administration Date: October 1992Award: $4,515

Title: Molecular Consequences of Tolerance and DependenceAgency: Foundation of UMDNJDate: July 1, 1993-June 30, 1994Award: $25,000

Title: Opioid Receptor Signal Transduction: Gene RegulationAgency: Biomedical Research Support GrantDate: April 1, 1994-May 31, 1995Award: $15,000

Title: Structure and Function of Delta Opioid ReceptorsAgency: SmithKline Beecham PharmaceuticalsDate: June 1996-May 1997Award: $4,990

Title: Structure/Function Analysis of Opioid ReceptorsAgency: National Institutes of Health/National Institute on Drug Abuse, DA09113Date: July 15, 1997-June 30, 2001Award: $501,763

Title: Regulation of Gene Expression Following Acute and Chronic Opioid Administration

Agency: NJMS Dean’s Biomedical Research Support Fund 200017

Howells, Richard D.

Date: October 1, 2000 - September 30, 2001Award: $15,000

Title: Mass Spectrometry of the Opioid Receptor*Agency: NIH/National Institute on Drug Abuse, F31 DA15285Date: February 2001- January 2002Award: $25,000* Served as sponsor and mentor for NRSA predoctoral fellowship to Keith Christoffers.

Title: Opioid Receptor Down Regulation: Role of the Proteasome Agency: Foundation of UMDNJDate: July 1, 2002-June 30, 2003Award: $35,000

Title: Purification and Mass Spectrometry of Opioid ReceptorsAgency: National Institutes of Health/Natl. Inst. on Drug Abuse, DA09113-04Date: April 1, 2003-March 31, 2008Award: $971,875

Title: Cross-talk between Opioid and Oncogenic Signaling in Human Multiple Myeloma – seed grant

Agency: Foundation of UMDNJ Date: July 1, 2007-June 30, 2008Award: $25,000

Title: Probing Mechanisms of Kappa Opioid Receptor Regulation using Mass Spectrometry”, F31 NRSA Predoctoral Fellowship

Agency: NIH/NIDA, Kenneth Wannemacher, PI, R.D. Howells, sponsor, DA024495.

Date: September 19, 2007-September 18, 2009Award: $28,770

Title: Training in Integrative Neuroscience in Health and DiseaseAgency: NIH/NINDS, T32 NS051157, Ellen Townes-Anderson, PhD, PI, R.D.

Howells, faculty mentorDate: July 1, 2007-June 30, 2012Award: $161,000 per year

Title: Multiple Myeloma Cell Growth Inhibition by an Opioid

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Howells, Richard D.

Agency: New Jersey State Commission on Cancer Research, Alexandra Terskiy, PI, R.D. Howells, sponsor and mentor

Date: July 1, 2008-June 30, 2010Award: $45,000

Title: The Ubiquitin/Proteasome Pathway and Opioid Receptor Down-Regulation – bridge grant

Agency: Foundation of UMDNJ Date: December 1, 2008- November 30, 2009Award: $25,000

Patent Application: UMDNJ invention Disclosure Form docket number: Dkt. 10-68; Novel Therapeutic Targets for Select Opioids, submitted by Elysa B. Goldberg, PhD, Esq., March 2013.

Title: Novel Discovery that Naltrindole Inhibits the Proliferation of Human Multiple Myeloma Cells

Agency: Office of Technology Transfer and Business Development, and the Foundation of UMDNJ

Date: July 1, 2013- September 1, 2013Award: $10,000

Title: Efficacy of Naltrindole and Related Compounds to Inhibit Multiple Myeloma Cell Growth In Vitro and In Vivo

Agency: The New Jersey Health Foundation, Inc. Annual Seed Grant Research Program

Date: July 2013Award: $10,000

Title: Rational Design, Synthesis and Testing of Small-Molecule Inhibitors of Multiple Myeloma

Agency: Rutgers Cancer Institute of New Jersey, Translational Synthesis Project, William Welsh (RWJMS-RU) and Richard D. Howells, PIs.

Award: Approved for funding on Sept 7, 2013; service contract worth approx. $4,000.

Grants Being Reviewed or Planned

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Howells, Richard D.

Discovery of Small-Molecule Oral Therapeutics for Multiple Myeloma, submitted March 31, 2014 to the 2014 Century for the Cure Research Award. Dr. William J. Welsh, PI and Dr. Richard D. Howells, Co-PI.

Project Team for the Discovery of Novel Therapeutics for Multiple Myeloma, submitted May 5, 2014 to research@ca.rutgers.edu. Dr. Richard D. Howells, PI and Dr. William J. Welsh, Co-PI.

In Vivo Study in Mice to Establish Oral Bioavailability of Naltrindole Prodrugs, submitted June 5, 2014 to the OTC Translational Research Mini-Grants for RBHS Inventions, William J. Welsh (RWJMS), Youyi Peng (RWJMS), and Richard D. Howells (NJMS), inventors.

Discovery of Novel Therapeutics for Multiple Myeloma, submitted March 17, 2015 as a Busch Biomedical Research Grant application, Dr. Richard D. Howells, PI and Dr. William J. Welsh, Co-PI.

Discovery of Novel Therapeutics for Multiple Myeloma, submitted May 8, 2015 to the New Jersey Health Foundation & The Nicholson Foundation Innovation Grants Program. Dr. Richard D. Howells, PI and Dr. William J. Welsh, Co-PI.

Patent Application: Invention Disclosure Docket RU S14-018: Title of Invention: Prodrugs of 4,8-Methanobenzofuro[2,3-a]pyrido[4,3-b]carbazole-1,8a(9H)-diol, 7-(cyclopropylmethyl)-5,6,7,8,14,14b-hexahydro-, (4bS,8R,8aS,14bR)- (Naltrindole) and Related Compounds and Therapeutic Uses Thereof, Richard D. Howells (NJMS), Youyi Peng (RWJMS), and William J. Welsh (RWJMS), inventors.

Patent Application: Title of Invention: Novel applications for the use of DOP ligands derived from naltrindole to treat human diseases. Richard D. Howells and William J. Welsh, PIs.

Patent Application: Invention Disclosure Form docket number NJMS 13-21 entitled “Non-opioid receptor-dependent effects of naltrindole on Ca signaling in human multiple myeloma cells”. Richard D. Howells, PI.

“Naltrindole inhibition of myeloma cell proliferation”, to be submitted to the NJ Commission on Cancer Research, R.D. Howells, PI

“Naltrindole inhibition of myeloma cell proliferation”, to be submitted to the Leukemia and Lymphoma Society, R.D. Howells, PI

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Howells, Richard D.

"The Ubiquitin/Proteasome Pathway and Opioid Receptor Down-Regulation", to be submitted to NIH/NIDA (competitive renewal of DA009113), RO1 mechanism, R.D. Howells, P.I.

“Naltrindole and related opioids inhibit multiple myeloma cell growth”, R.D. Howells, Principal Investigator, to be submitted to NIH.

b. Co-Principal Investigator

Title: Molecular and Cellular Basis of EpileptogenesisAgency: National Institutes of HealthDate: April 1990-March 1994Award: $600,000

Title: Establishment of a UMDNJ Neuroproteomics Core Facility, R.D. Howells, Co-investigator, Hong Li, P.I.

Agency: NIH/NINDSDate: December 2004-November 2009Award: $3,833,775

16. MAJOR TEACHING EXPERIENCE

Graduate Teaching Assistant Organic Chemistry

BiochemistryPharmacology

Faculty Lecturer

1988-present Dental BiochemistryMedical Biochemistry; Molecular and Genetic MedicineGraduate Biochemistry Bioc-5308--Current Topics in BiochemistryAnat-5300--Graduate Course in Advanced Cell BiologyBioc-5317--Analytical Methods in Biochemistry

1991-present Bioc-5200--Regulation of Gene Expression, Course Director1991-2000 Bioc-507A--Structure of Nucleic Acids

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Howells, Richard D.

1993-1999 Anat-5330--Cell Biology of the Host Response to Injury

1997-2001 Participant in NJMS FIRST Pre-matriculation Program

1999-present Molecular Biology of the NewsNucleic Acids BiochemistryGSBS Molecular and Cellular Biology Core Curriculum

2003-2011 Course Director—MD/PhD Seminar Club

2003-present Molecular and Genetic MedicineUnit 2 Leader – 2012-presentBiochem. Problem Sessions Leader – 2013-present

2006-present Foundations of Biochemistry and Molecular Biology

2008-present Protein Dynamics in Health and DiseaseSeminar in Biomedical Science

2010-2014 Genetics and Molecular MedicineUnit 2 Leader

2011-2014 Enzyme Inhibitors in Drug Discovery

2012-2014 Unit Leader, Molecular and Genetic Medicine

2013-2014 Director, Molecular and Genetic Medicine Biochemistry Problem Sessions

Fascilitator for Dental Biochemistry Problem-based Learning

Fundamentals of Biomedical Sciences A- Biochemistry and Molecular Biology, Co-Course Director

2014-present Dental Biochemistry, Course Director

Ph.D. Thesis Mentor

1988-1992 Ms. S. Rao - received Ph.D. 12/92

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Howells, Richard D.

1990-1992 Ms. J. Yin - received Ph.D. 11/92

1990-1995 Mr. L. Scala - received Ph.D. 10/95

1992-1994 Mr. Deba Saha - received Ph.D. 10/94

1993-1995 Mr. William Wang - received Ph.D. 10/95

1993-1995 Ms. Mandana Shahrestanifar - received Ph.D. 12/95

1997-2003 Mr. Keith Christoffers*- Dept. of Neuroscience-received Ph.D. 7/03*Recipient of the NJMS Faculty Organization Outstanding Student Award

2005-2008 Mr. Kenneth Wannemacher – received PhD 09/08*Recipient of the Dr. Joseph Fu Award, 2008

2006-2009 Ms. Alexandra Terskiy – MD/PhD Program – received PhD 06/09*Recipient of the Dean Morris Schafer Award, 2008*Recipient of the Philanthropic Education Organization Scholar Award, 2009

2010-2012 Ms. Jyoti Joshi, Interdisciplinary Program (married name, Mundra)- received PhD 12/12

Postdoctoral Fellow Mentor

1992-1994 Dr. Padmashree Tirumalai

1998-2000 Dr. Persis Bandari

1998-2001 Dr. Kirti Chaturvedi

2002-2005 Dr. Susan Keenan

2003-2005 Dr. Keith Christoffers

2003-2007 Dr. Prem Yadav23

Howells, Richard D.

M.S. Thesis Mentor

2000-2001 Mr. Mathew Wolenski

2004-2006 Ms. Madonna Doligosa

2010-2012 Ms. Veronika Khariv

2012-present Ms. Julianne Avrutik

Visiting Scholar Mentor

1999-2000 Dr. Xiuju Jiang

Mentor for Medical Student Research Fellowships

1992 Kathleen Kelly

1994 Tonya Solis Nina Brugel

1996 Rajan Ravikumar

1997 Eric Edwards

2009 Erica Pimenta

2010 Heather Holahan

2011 Brittany Gladney

2013 Andrew Meillier

2014 Pankti Kothari

Mentor for Graduate Student Research Rotations

1990 Louis Scala

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Howells, Richard D.

1991 Jun Yin

1992 William WangYou-Zhen Wang

1993 Amartya BasuDeba Saha

1994 Weikang TaoJin JinKathy Piparo

1995 Peter SunenshineSyed RezaTheophilus Awuah

1996 Michael HueningKeith Christoffers

1997 Cindy Jo ArrigoXie XinNorihiro Chinen

1998 Wei SunElie Needle

1999 Ling Kang

2000 Mathew Wolenski

2003 Kenneth WannemacherMadonna Doligosa

2004 Walid Abushahba

2005 Murugabaskar BalanAlexandra Terskiy (MD/PhD Program)

2006 Alexandra Terskiy (MD/PhD Program)

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Howells, Richard D.

2009 Jyoti Joshi

2011 Veronica Khariv

2013 Thomas Choongnam YoonEmily Chan

2014 Mona Rahimi-TareeTarundeep SinghAmanjot Sarao

2015 Amanda PenaParvati GopolanPooja Pankaj KothariRobert Zielinski

Mentor for Undergraduate Student Research Rotations

2000 Arshia Khokhar

2000 Norihiro Chinen

2011 Menaka Dhingra

2012 Alessandro Howells

2014 Pankto Kothari

Mentor for High School Student Research Rotations

2009 Cassandra Farnow

2010 Menaka Dhingra

2011 Pallivi Koppol

2012 Giovanni Howells

17. PRINCIPAL CLINICAL AND HOSPITAL SERVICE RESPONSIBILITIES -- N/A

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18. MAJOR ADMINISTRATIVE RESPONSIBILITIES

2003-2011 Assistant Dean for the MD/PhD Program at UMDNJ-NJMS

2014- present Member of the Rutgers Biomedical and Health Sciences Faculty Council

19. PRIVATE PRACTICE -- N/A

OTHER SCHOLARLY ACTIVITIES

PROFESSIONAL WORKSHOPS

Participated in the 2013 International Association of Medical Science Educators (IAMSE) Faculty Development Webinar Series

2012-present Participated in the NJMS Research Office and Core Facilities Technology Lecture Series Program

PATENT APPLICATIONS

UMDNJ invention disclosure docket number: Dkt. 10-68; Novel Therapeutic Targets for Select Opioids, submitted by Elysa B. Goldberg, PhD, Esq., March 2013.

Patent Application: Invention Disclosure Form docket number NJMS 13-21 entitled “Non-opioid receptor-dependent effects of naltrindole on Ca signaling in human multiple myeloma cells”. Richard D. Howells, PI.

Patent Application: Docket RU S14-018 - Prodrugs of 4,8-Methanobenzofuro[2,3-a]pyrido[4,3-b]carbazole-1,8a(9H)-diol, 7-(cyclopropylmethyl)-5,6,7,8,14,14b-hexahydro-, (4bS,8R,8aS,14bR)- (Naltrindole) andRelated Compounds and Therapeutic Uses Thereof

Patent Application: Title of Invention: Novel applications for the use of MM triazole ligands derived from naltrindole to treat human diseases. Richard D. Howells and William J. Welsh, PIs.

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Howells, Richard D.

INVITED LECTURER

1984 "Proenkephalin Biosynthesis in the Rat," presented at the International Conference on the Medicinal Chemistry and Molecular Biology of the Opioid Peptides and the Opiates, National Institute on Drug Abuse, Bethesda, MD

"Regulation of Proenkephalin Biosynthesis," presented at the Thirtieth National Congress of the Italian Biochemical Society, Lacco Ameno d'Ischia, Italy

1986 "Cloning and Sequence Analysis of Rat Proenkephalin cDNA: Probe for Studying Transcription in Brain, Heart, Adrenal and Gonads," Behavioral Biology Seminar Series, Rockefeller University, New York, NY

1987 "Studies on the Distribution of Proenkephalin and the Regulation of its Biosynthesis," presented at the 1987 Gordon Research Conference on "Mode of Action of Opiates", Santa Barbara, CA

"Tissue-specific Expression and Regulation of the Proenkephalin Gene," presented at the 1987 Biotechnology Symposium on "The Emergence of Molecular Neurobiology," East Carolina School of Medicine, Greenville, NC

1988 "Regulation of Proenkephalin Gene Expression," presented to the Department of Pharmacology, UMDNJ-New Jersey Medical School

1990 "Tissue-specific Splicing of Proenkephalin mRNA," presented at the Fifty-Second Annual Meeting of the Committee on Problems of Drug Dependence, June 10-14, 1990 in Richmond VA

"Opioids: Peptide Gene Products, Receptors, and Addiction," presented at the National Institute on Drug Abuse, September 14, 1990

1991 "Regulation of Proenkephalin Expression in the Brain and Periphery," presented at the Mario Negri Institute of Pharmacological Research, Milan, Italy, September 5, 1991

1992 "Regulation of Proenkephalin and c-Fos Gene Expression in C6 Glioma Cells," presented at the Mario Negri Institute of Pharmacological Research, Milan, Italy, December 14, 1992

"Regulation of c-Fos Gene Expression by Endothelin," presented at the Mario Negri Research Institute, Bergamo, Italy, December 22, 1992

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1993 "Regulation of Proenkephalin and c-Fos Gene Expression," presented at the Department of Physiology, UMDNJ-New Jersey Medical School, Newark, NJ, April 16, 1993

"Regulation of Proenkephalin and c-Fos Gene Expression," presented at Bristol Myers-Squibb Pharmaceutical Research Institute, Princeton, NJ, May 7, 1993

1994 "Proenkephalin Expression in Rat Testis and Brain," Presented at CUNY, New York, April 15, 1994

1995 "Structure Function Analysis of Opioid Receptors," presented at Department of Surgery, UMDNJ-New Jersey Medical School, October 20, 1995

"G Protein-coupled Receptors," presented at New York University Medical Center, November 16, 1995

"Molecular Analysis of Opioid Receptor Structure and Function," presented at the Department of Pharmacology, New York University Medical Center, November 16, 1995

1996 “Studies on Opioid Receptor Structure and Function,” presented at the Department of Pharmacology, Temple University School of Medicine, Philadelphia, PA, January 24, 1996

“Site-directed Mutagenesis and Chimeric Analysis of Opioid Receptors,” presented at the Mario Negri Institute of Pharmacological Research, Milan, Italy, June 24, 1996

“Molecular Analysis of Opioid Receptor Structure and Function,” presented at the Department of Neuroscience, UMDNJ-New Jersey Medical School, September 30, 1996

“Signal Transduction by Activated G Protein-coupled Receptors,” presented at Department of Microbiology, New York University Medical Center, November 19, 1996

1997 “Site-directed Mutagenesis of the Opioid Receptor Binding Site,” presented at Smith Kline Beecham, SpA, Baranzate, Italy, January 3, 1997

“Recent Advances on Structure/Function Relationships of Opioid Receptors,” presented at the Department of Biochemistry and Molecular Biology, UMDNJ-New Jersey Medical School, April 10, 1997

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“Structural and Functional Principles of Membrane Receptors: G Protein-Coupled Receptors,” presented at the Department of Biochemistry, New York University Medical Center, November 18, 1997

“Ligand Binding, Signal Transduction, and Down-Regulation of Opioid Receptors,” presented at the Mario Negri Institute of Pharmacological Research, Milan, Italy, December 22, 1997

1998 “The Ups and Downs of Opioid Receptor Signaling: Activation and Down-Regulation,” presented at the Department of Pharmacology and Physiology,

UMDNJ-New Jersey Medical School, October 26, 1998

“G Protein-Coupled Receptors: Ligand Binding and Signal Transduction,” presented at the Sackler Institute of Graduate Biomedical Sciences, New York

University Medical Center, December 1, 1998

1999 “Molecular Basis of Tolerance and Dependence,” presented at the Pain Management Center, UMDNJ-New Jersey Medical School, June 18, 1999

“G Protein-Coupled Receptors: Methods to Characterize the Ligand Binding Site” presented at the Sackler Institute of Graduate Biomedical Sciences, New York University Medical Center, November 30, 1999

2000 “Structure/Function Analysis of the Delta Opioid Receptor”, presented at SmithKline Beecham, S.p.A., Milan, Italy, June 19, 2000

"G Protein-Coupled Receptors: Activation and Signaling", presented in the Department of Biochemistry, New York University Medical Center, November 28, 2000.

2002 "Life as an Academic Biochemist", presented at Career Day, Shongum Elementary School, Randolph, NJ, April 5, 2002.

"Analysis of Opioid Receptors by Mass Spectrometry", presented in the Department of Biochemistry and Molecular Biology, UMDNJ-NJMS, October 4, 2002.

"Analysis of Purified Opioid Receptors using Mass Spectrometry", presented at the Mario Negri Institute for Pharmacological Research, Milan, Italy, December 16, 2002.

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2003 "Post-translational Modifications of Opioid Receptors Determined by Mass Spectrometry", presented at the 2003 International Narcotic Research Conference, Perpignan, France, July 6-11, 2003.

“Purification and Mass Spectrometric Analysis of the Mu Opioid Receptor”, presented at the MD/PhD Seminar Series, UMDNJ-NJMS, September 17, 2003.

“Structure and Regulation of Opioid Receptors”, presented at the UMDNJ-GSBS Seminar Series, September 24, 2003.

2004 “The M.D./Ph.D. Program at NJMS”, presented at the Accepted Students Luncheon, UMDNJ-NJMS, January 9, 2004.

“Characterization of Opioid Receptors”, presented at Purdue Pharma, Cranbury, NJ on April 23, 2004.

“The Dual MD/PhD Program at NJMS”, presented at the Accepted Students Luncheon, UMDNJ-NJMS, June 4, 2004.

“Regulation of Opioid Receptors by Agonists and Antagonists”, presented at the Department of Biochemistry and Molecular Biology, UMDNJ-NJMS, November 5, 2004.

2005 “The M.D./Ph.D. Program at NJMS”, presented at the Accepted Students Luncheon, UMDNJ-NJMS, January 7, 2005.

“Research Opportunities at NJMS”, presented at the Accepted Students Luncheon, UMDNJ-NJMS, June 3, 2005.

2006 “Regulation of Opioid Receptor Expression and Function”, presented at Saint John’s University, NY, March 30, 2006.

“Regulation of Opioid Receptor Expression and Function”, presented at Temple University School of Medicine, April 26, 2006.

2007 “Regulation of Opioid Receptor Expression and Signaling”, presented at Stevens Institute of Technology, February 28, 2007.

“Confessions of an English (American) Opium Researcher”, presented at UMDNJ-New Jersey Medical School, March 23, 2007.

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2008 “Differential Regulation of Delta Opioid Receptor Expression by Histone Deacetylase Inhibitors”, presented at the Society for Neuroscience annual meeting, Washington DC November 19, 2008.

2009 “Novel Treatment of Multiple Myeloma”, presented for Seminars in Biomedical Sciences, GSBS, September 29, 2009.

2010 “Novel Treatment of Multiple Myeloma”, presented for Seminars in Biomedical Sciences, GSBS, September 28, 2010.

2011 “Inhibition of human multiple myeloma cell proliferation by naltrindole”, presented at the International Narcotic Research Conference, Hollywood, FL, June 24, 2011.

“Novel Treatment of Multiple Myeloma”, presented for Seminars in Biomedical Sciences, GSBS, September 27, 2011.

“Inhibition of human multiple myeloma cell proliferation by naltrindole”, presented to the Fox Chase Chemical Diversity Center and UMDNJ Office of Technology Transfer and Business Development, Somerset, NJ, September 28, 2011.

“Therapeutic Potential of Opioids for the Treatment of Cancer”, presented to the Dept. of Biochemistry and Molecular Biology, UMDNJ-NJMS, November 11, 2011.

2012 “Novel Treatment of Multiple Myeloma”, presented for Seminars in Biomedical Sciences, GSBS, September 25, 2012.

2013 “Molecular modeling of naltrindole led to the discovery of novel triazole-based inhibitors of the proliferation of human multiple myeloma cells”. Presented at the annual meeting of the Cancer Institute of New Jersey, Piscataway, NJ, May 2013.

“Novel Treatments for Multiple Myeloma”, invited presentation for GSBS 5910Q Seminars in Biomedical Sciences, Newark, NJ, September 24, 2013.

“Naltrindole as a therapeutic agent for the treatment of multiple myeloma”, invited presentation in the Department of Biochemistry and Molecular Biology Seminar series, Newark, NJ, November 8, 2013.

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2014 “Novel small molecule triazoles modeled from naltrindole potently inhibit the proliferation of human multiple myeloma cells”. Presented at the annual meeting of the Cancer Institute of New Jersey, Piscataway, NJ, May 2014.

“Novel small molecule triazoles modeled from naltrindole potently inhibit the proliferation of human multiple myeloma cells”, presented at the International Narcotic Research Conference, Montreal, Canada, July 14, 2014.

“Naltrindole and trisubstituted triazoles modeled from naltrindole as novel therapeutic agents to treat multiple myeloma”. Invited presentation for GSBS 5910Q Seminars in Biomedical Sciences, September 25, 2014.

2015 “Discovery of Novel Therapeutics for Multiple Myeloma”. Invited presentation to the New Jersey Health Foundation & The Nicholson Foundation Innovation Grants Program, May 27, 2015.

BIBLIOGRAPHY

20. ARTICLES

1. Howells, R. D., Hiller, J. M. and Simon, E. J. Benzodiazepine binding sites are present in retina. Life Sci. 25: 2131-2136, 1979.

2. Howells, R. D., Groth, J., Hiller, J. M. and Simon, E. J. Opiate binding sites in the retina: Properties and distribution. J. Pharmacol. Exp. Ther. 215: 60-64, 1980.

3. Howells, R. D. and Simon, E. J. Benzodiazepine binding in chicken retina and its interaction with gamma-aminobutyric acid. Eur. J. Pharmacol. 67: 133-137, 1980.

4. Ruegg, U. T., Cuenod, S., Hiller, J. M., Gioannini, T., Howells, R. D. and Simon, E. J. Characterization and partial purification of solubilized active opiate receptors from toad brain. Proc. Natl. Acad. Sci. USA 78: 4635-4638, 1981.

5. Howells, R. D., Gioannini, T. L., Hiller, J. M. and Simon, E. J. Solubilization and characterization of active opiate binding sites from mammalian brain. J. Pharmacol. Exp. Ther. 222: 629-634, 1982.

6. Gioannini, T. L., Howells, R. D., Hiller, J. M. and Simon, E. J. Solubilization in good yield of active opiate binding sites from mammalian brain. Life Sci. 31: 1315-1318, 1982.

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7. Kilpatrick, D. L., Howells, R. D., Lahm, H.-W. and Udenfriend, S. Evidence for a proenkephalin-like precursor in amphibian brain. Proc. Natl. Acad. Sci. USA 80: 5772- 5775, 1983.

8. Kilpatrick, D. L., Howells, R. D., Fleminger, G. and Udenfriend, S. Denervation of rat adrenal glands markedly increases preproenkephalin mRNA. Proc. Natl. Acad. Sci. USA 81: 7221-7223, 1984.

9. Howells, R. D., Kilpatrick, D. L., Bhatt, R., Monahan, J. J., Poonian, M. and Udenfriend, S. Molecular cloning and sequence determination of rat preproenkephalin cDNA: Sensitive probe for studying transcriptional changes in rat tissues. Proc. Natl. Acad. Sci. USA 81: 7651-7655, 1984.

10. Fleminger, G., Howells, R. D., Kilpatrick, D. L. and Udenfriend, S. Intact proenkephalin is the major enkephalin-containing peptide produced in rat adrenal glands following denervation. Proc. Natl. Acad. Sci. USA 81: 7985-7988, 1984.

11. Kilpatrick, D. L., Howells, R. D., Noe, M., Bailey, L. C. and Udenfriend, S. Expression of preproenkephalin-like mRNA and its peptide products in mammalian testis and ovary. Proc. Natl. Acad. Sci. USA 82: 7467-7469, 1985.

12. Howells, R. D., Kilpatrick, D. L., Noe, M., Bailey, L. C. and Udenfriend, S. Proenkephalin mRNA in rat heart. Proc. Natl. Acad. Sci. USA 83: 1960-1963, 1986.

13. Shivers, B. D., Harlan, R. E., Romano, G. J., Howells, R. D. and Pfaff, D. W. Cellular localization of proenkephalin mRNA in rat brain: Gene expression in the caudate-putamen and cerebellar cortex. Proc. Natl. Acad. Sci. USA 83: 6221-6225, 1986.

14. Romano, G. J., Shivers, B. D., Harlan, R. E., Howells, R. D. and Pfaff, D. W. Haloperidol increases proenkephalin mRNA levels in the caudate-putamen of the rat: A quantitative study at the cellular level using in situ hybridization. Molec. Brain Res. 2: 33-41, 1987.

15. Harlan, R. E., Shivers, B. D., Romano, G. J., Howells, R. D. and Pfaff, D. W. Cellular localization of proenkephalin mRNA in the rat neuraxis brain and spinal cord by in situ hybridization. J. Comp. Neurol. 258: 159-184 (1987).

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16. Inturrisi, C. E., Branch, A. D., Robertson, H. D., Howells, R. D., Franklin, S. O., Shapiro, J. R., Calvano, S. E. and Yoburn, B. C. Glucocorticoid regulation of enkephalins in cultured rat adrenal medulla. Mol. Endocrinol. 2: 633-640, 1988.

17. Romano, G. J., Mobbs, C. V., Howells, R. D. and Pfaff, D. W. Estrogen increases

proenkephalin messenger ribonucleic acid levels in the ventromedial hypothalamus of the rat. Mol. Endocrinol. 2: 1320-1328, 1988.

18. Romano, G. J., Mobbs, C. V., Howells, R. D. and Pfaff, D. W. Estrogen regulation of proenkephalin gene expression in the ventromedial hypothalamus of the rat: Temporal qualities and synergism with progesterone. Mol. Brain Res. 5: 51-58, 1989.

19. Lauber, A. H., Romano, C. J., Mobbs, C. V., Howells, R. D. and Pfaff, D. W. Estradiol induction of proenkephalin messenger RNA in hypothalamus: Dose response and relation to reproductive behavior in the female rat. Mol. Brain Res. 8: 447-54, 1990.

20. Romano, G. J., Mobbs, C. V., Lauber, A., Howells, R. D. and Pfaff, D. W. Differential regulation of proenkephalin gene expression by estrogen in the ventromedial hypothalamus of male and female rats: Implications for the molecular basis of a sexually differentiated behavior. Brain Res. 536: 63-68, 1990.

21. Yin, J. and Howells, R. D. Glucocorticoid-mediated down regulation of c-fos mRNA in C6 glioma cells: Lack of correlation with proenkephalin mRNA. Mol. Brain Res. 12: 187-194, 1992.

22. Yin, J., Lee, J. A. and Howells, R. D. Stimulation of c-fos and c-jun gene expression and down-regulation of proenkephalin gene expression in C6 glioma cells by endothelin. Mol. Brain Res. 14: 213-220, 1992.

23. Rao, S. M. and Howells, R. D. Molecular cloning, sequence analysis and translation of proenkephalin mRNA from rat heart. Reg. Peptides 40: 397-408, 1992.

24. Eiring, A., Manier, D. H., Bieck, P. R., Howells, R. D. and Sulser, F. The "serotonin/norepinephrine link" beyond the beta adrenoceptor. Mol. Brain Res. 16: 211-214, 1992.

25. Saha, D., Tirumalai, P., Scala, L. and Howells, R. D. Human ribosomal protein L37a: Cloning of the cDNA and analysis of differential gene expression in human and rat tissues and cell lines. Gene 132: 285-289, 1993.

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26. Rao, S. M. and Howells, R. D. cis-Acting elements in the 5'-untranslated region of rat testis proenkephalin mRNA regulate translation of the precursor protein. J. Biol. Chem. 268: 22164-22169, 1993.

27. Tirumalai, P. and Howells, R. D. Regulation of calbindin- D28k gene expression in response to acute and chronic morphine administration. Mol. Brain Res. 23: 144-150, 1994.

28. Lee, S., Miskovsky, J., Williamson, J., Howells, R. D., Devinsky, O., Lothman, E. and Christakos, S. Changes in glutamate receptor and proenkephalin gene expression after kindled seizures. Mol. Brain Res. 24: 34-42, 1994.

29. Wang, W. W. and Howells, R. D., Sequence of the 5'-flanking region of the rat c-fos proto-oncogene. Gene 143: 261-264, 1994.

30. Shahrestanifar, M., Saha, D. P., Scala, L. A., Basu, A. and Howells, R. D. Cloning of a human cDNA encoding a putative nucleotide-binding protein related to

Escherichia coli MinD. Gene 147: 281-285, 1994.

31. Wang, W. W., Shahrestanifar, M. and Howells, R. D. Studies on mu and delta opioid receptor selectivity utilizing chimeric and site-mutagenized receptors. Proc. Natl. Acad. Sci. USA 92: 12436-12440, 1995.

32. Shahrestanifar, M., Wang, W. W. and Howells, R. D. Studies on inhibition of mu and

delta opioid receptor binding by dithiothreitol and N-ethylmaleimide: His223 is critical for mu opioid receptor binding and inactivation by N-ethylmaleimide. J. Biol. Chem. 271: 5505-5512, 1996.

33. Shahrestanifar, M. and Howells, R. D. Sensitivity of opioid receptor binding to N-substituted maleimides and methanethiosulfonate derivatives. Neurochem. Res. 21: 1295-1299, 1996.

34. Wu, G., Lu, Z. H., Wei, T. J., Howells, R. D., Christoffers, K. and Ledeen, R. W. The role of GM1 ganglioside in regulating excitatory opioid effects. Ann. N. Y. Acad. Sci. 845:126-38, 1998.

35. Cai, H., Howells, R.D. and Wagner, B.J. Identification of a novel gene product preferentially expressed in rat lens epithelial cells. Molecular Vision 5:3 http://www.molvis/v5/p3, 1999.

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36. Chaturvedi, K., Shahrestanifar, M. and Howells, R. D. Mu opioid receptor: role for the amino terminus as a determinant of ligand binding affinity. Mol. Brain Res. 76: 64-72, 2000.

37. Chaturvedi, K., Jiang, X., Christoffers, K. H., Chinen, N., Bandari, P., Raveglia, L. F., Ronzoni, S., Dondio, G. and Howells, R. D. Pharmacological profiles of selective non-peptidic delta opioid receptor ligands. Mol. Brain Res. 80: 166-176, 2000.

38. Chaturvedi, K., Bandari, P., Chinen, N., and Howells, R. D. Proteasome involvement in agonist-induced down regulation of mu and delta opioid receptors. J. Biol. Chem. 276: 12345-12355, 2001.

39. Christoffers, K.H., Khohkar, A., Chaturvedi, K. and Howells, R.D. Inhibition of mu and delta opioid receptor ligand binding by the peptide aldehyde protease inhibitor, leupeptin. Regulatory Peptides, 105: 9-14, 2002.

40. Christoffers, K.H., Li, H., Keenan, S.M. and Howells, R.D. Purification and mass spectrometric analysis of the opioid receptor. Mol. Brain Res. 118: 119-131, 2003.

41. Christoffers, K.H., Li, H. and Howells, R.D. Purification and mass spectrometric analysis of the opioid receptor. Mol. Brain Res., 136: 54-64, 2005.

42. Zhang, Q., Keenan, S.M., Peng, Y., Nair, A.C., Yu, S.-J., Howells, R.D., and Welsh, W.J. Discovery of novel triazole-based opioid receptor antagonists. J. Med. Chem., 49: 4044-4047, 2006.

43. Yadav, P., Chaturvedi, K. and Howells, R.D. Inhibition of agonist-induced down regulation of the delta opioid receptor with a proteasome inhibitor attenuates opioid tolerance in human embryonic kidney 293 cells. J. Pharmacol. Exp. Ther., 320: 1186-1194, 2007.

44. Wannemacher, K.M., Yadav, P.N. and Howells, R.D. A select set of opioid ligands induce up regulation by promoting the maturation and stability of the rat kappa opioid receptor in human embryonic kidney 293 cells. J. Pharmacol. Exp. Ther., 323: 614-625, 2007.

45. Terskiy, A., Wannemacher, K.M., Yadav, P.N., Tian, B. and Howells, R.D. Search of the human proteome for endomorphin-1 and endomorphin-2 precursor proteins. Life Sciences, 81: 1593-1601, 2007.

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46. Wannemacher, K.M., Terskiy, A., Bian, S., Yadav, P.N., Li, H., and Howells, R.D. Purification and mass spectrometric analysis of the opioid receptor. Brain Res. 1230: 13-26, 2008.

47. Peng, Y., Zhang, Q., Arora, S., Keenan, S.M., Kortagere, S., Wannemacher, K.M., Howells, R.D. and Welsh, W.J., Novel delta opioid receptor agonists exhibit differential stimulation of signaling pathways. Bioorg. Med. Chem., 17: 6442-6450, 2009.

48. Mundra, J.J., Terskiy, A. and Howells, R.D., Naltrindole inhibits human multiple myeloma cell proliferation in vitro and in a murine xenograft model in vivo. J. Pharmacol. Exp. Ther., 342: 273-287, 2012.

21. BOOKS, MONOGRAPHS AND CHAPTERS

1. Simon, E. J., Hiller, J. M., Gioannini, T. L., Howells, R. D. and Ruegg, U. T. Characterization of solubilized active opiate receptors from toad brain. In "Advances in Endogenous and Exogenous Opioids" (H. Takagi and E. J. Simon, eds.) Kodanska Ltd. and Elsevier Biomedical, Tokyo and Amsterdam, pp. 68-70 (1981).

2. Simon, E. J., Hiller, J. M., Ruegg, U. T., Gioannini, T., Howells, R. D., Groth, J., Angel, L. and Bonnet, K. H. Recent work on opiate receptors: Heterogeneity and solubilization. In "Regulatory Peptides: From Molecular Biology to Function" (E. Costa and M. Trabucci, eds.) Adv. Biochem. Psychopharmacol., vol. 33, Raven Press, New York (1982).

3. Hiller, J. M., Simon, E. J., Gioannini, T. L. and Howells, R. D. Solubilization and characterization of active opiate binding sites. In "Investigation of Membrane-located Receptors" (E. Reid, G. M. W. Cook and D. J. Moore, eds.) Plenum Publishing Corp., New York, pp. 445-453 (1984).

4. Howells, R. D. Proenkephalin biosynthesis in the rat. In "Opioid Peptides: Molecular Pharmacology, Biosynthesis, and Analysis" (R. S. Rapaka and R. L. Hawks, eds.) National Institute on Drug Abuse Research Monograph Series, vol. 70, Supt. of Docs., Govt. Print Off., Washington, DC, pp. 43-65 (1986).

5. Shivers, B. D., Harlan, R. E., Romano, G. J., Howells, R. D. and Pfaff, D.W. Cellular location and regulation of proenkephalin mRNA in rat brain. In "In Situ Hybridization in Brain" (G. R. Uhl, ed.) Plenum Publishing Corp., New York, pp. 3-20 (1986).

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6. Howells, R. D. and Rao, S. Tissue-specific splicing of proenkephalin mRNA. In NIDA Research Monograph (L. Harris, ed.) vol. 105, Govt. Print Off., Washington, DC, pp. 116-122 (1990).

7. Tirumalai, P. and Howells, R. D. Regulation of glutamate R3 receptor mRNA in rat cerebellum in response to acute and chronic morphine. In NIDA Research Monograph (L. Harris, ed.) vol. 141, Govt. Print Off., Washington, DC, p. 93 (1994).

8. Shahrestanifar, M. and Howells, R. D. Sensitivity of mu and delta opioid receptor binding to N-ethylmaleimide. In Regulatory Peptides 54: 269-270, 1994.

9. Unterwald, E.M. and Howells, R.D. Upregulation of opioid receptors. In Opioid receptors and antagonists: From bench to clinic. Contemporary Neuroscience series. Eds. Reginald L. Dean III, Edward J. Bilsky, and S. Steven Negus III, Humana Press, a part of Springer Science + Business Media, LLC Totowa, NJ, 2009.

22. ABSTRACTS

1. Howells, R. D., Gioannini, R. L., Hiller, J. M. and Simon, E. J. Solubilization of active opiate binding sites from mammalian Brain. Proc. Am. Soc. Pharmacol. Exp. Ther., New Orleans (1982).

2. Fleminger, G., Ezra, E., Howells, R., Kilpatrick, D. L. and Udenfriend, S. The effect of denervation on enkephalin-containing peptides and catecholamines in rat adrenal glands. Symposium on Molecular and Cellular Biology: Protein Transport and Secretion, Keystone, CO (1983).

3. Kilpatrick, D. L., Howells, R., Otis, L., Cai, J.-K. and Udenfriend, S. Identification of [Met]enkephalin, [Leu]enkephalin and [Met]enkephalin-Arg-Phe in toad brain. International Narcotic Research Conference, Garmisch, West Germany (1983).

4. Howells, R. D. and Udenfriend, S. Regulation of proenkephalin biosynthesis. 30th National Congress of the Italian Biochemical Society, Lacco Ameno d'Ischia, Italy (Oct. 7-10, 1984).

5. Howells, R. D., Kilpatrick, D. L., Noe, A., Fleminger, G., Bailey, L. C. and Udenfriend, S. Products of proenkephalin transcription and translation in mammalian heart. Fed. Proc. 44, 420 (1985).

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6. Howells, R. D., Kilpatrick, D. L., Noe, A., Bailey, L. C. and Udenfriend, S. Proenkephalin gene expression in rat heart. International Narcotic Research Conference, North Falmouth, MA (June 23-27, 1985).

7. Harlan, R. E., Shivers, B. D., Romano, G. J., Howells, R. D. and Pfaff, D. W. Localization of cells containing preproenkephalin mRNA in the rat forebrain by in situ hybridization. Society for Neuroscience, Dallas, TX (1985).

8. Shivers, B. D., Harlan, R. E., Romano, G. J., Howells, R. D. and Pfaff, D. W. Cellular localization of proenkephalin mRNA-containing neurons in rat brainstem and spinal cord. International Narcotic Research Conference, San Francisco, CA (July 6-11, 1986).

9. Howells, R. D., Shivers, B. D., Morris, P. and Udenfriend, S. Preproenkephalin mRNA levels in testis unaltered by hypophysectomy. International Narcotic Research Conference, San Francisco, CA (July 6-11, 1986).

10. Romano, G. J., Shivers, B. D., Harlan, R. E., Howells, R. D. and Pfaff, D. W. Haloperidol increases proenkephalin mRNA levels in the caudate-putamen of the rat: A quantitative study using in situ hybridization. International Narcotic Research Conference, San Francisco, CA (July 6-11, 1986).

11. Howells, R. D., Sun, R., Blume, A. J. and Udenfriend, S. Rat C6 glioma cells contain abundant amounts of preproenkephalin mRNA and methionine enkephalin. Society for Neuroscience, Washington, D.C. (Nov. 9-14, 1986).

12. Romano, G. J., Harlan, R. E., Shivers, B. D. Howells, R. D. and Pfaff, D. W. Estrogen increases proenkephalin mRNA levels in the mediobasal hypothalamus of the rat. Society for Neuroscience, Washington, D.C. (Nov. 9-14, 1986).

13. Romano, G. J., Howells, R. D. and Pfaff, D. W. Proenkephalin mRNA levels in the ventromedial hypothalamus of the rat increase rapidly after estrogen treatment. The Endocrine Society, Indianapolis, IN (June 10-12, 1987).

14. Inturrisi, C. E., Howells, R. D., Franklin, S. O., Shapiro, J. R., Calvano, S. E. and Yoburn, B. C. Rat adrenal medullary enkephalins and mRNA are increased by dexamethasone. Society for Neuroscience, New Orleans, LA (Nov. 16-21, 1987).

15. Rao, S. and Howells, R. D. Molecular cloning of proenkephalin (PE) mRNA from rat testis. American Society of Biochemistry and Molecular Biology, New Orleans, LA (June 4-7, 1990), FASEB J. 4: A1817, 1990.

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16. Canady, J., Hobson, R. W., Howells, R. D., Duran, W. N., Yasuhara, H., Watanabe, B. I. and Neville, R. F. Naloxone reverses no-reflow in postischemic skeletal muscle. Association for Academic Surgery, Houston, TX (Nov. 14-17, 1990).

17. Yin, J. and Howells, R. D. Temporal expression of c-fos and proenkephalin mRNA in C6 glioma cells. American Society of Cell Biology, San Diego, CA (December 9-13, 1990), J. Cell Biol. 111: 506a, 1990.

18. Rao, S. M. and Howells, R. D. Molecular cloning and sequencing of brain-type proenkephalin cDNA from rat testis. The Endocrine Society, Washington, DC (June 19-22, 1991), Endocrine Soc. Abstracts, pg. 74, 1991.

19. Tirumalai, P. and Howells, R. D. Effect of chronic morphine treatment on proenkephalin gene expression in the rat. The Endocrine Society, Washington, DC (June 19-22, 1991), Endocrine Soc. Abstracts, pg. 74, 1991.

20. Yin, J. and Howells, R. D. Endothelin-induced regulation of gene expression in rat C6 glioma cells. American Society of Cell Biology, Boston, MA (December 8-12, 1991). J. Cell Biol. 115: 26a, 1991.

21. Tirumalai, P. and Howells, R. D. Regulation of c-fos and 28K calbindin mRNA in rat cerebellum in response to acute and chronic morphine. Society for Neuroscience, Anaheim, CA (October 25-30, 1992), Soc. Neurosci. Abstracts 18: 992, 1992.

22. Lee, S., Miskovsky, J., Williamson, J., Howells, R., Lothman, E. and Christakos, S. Changes in glutamate receptor and proenkephalin expression after kindled seizures. Society for Neurosciences, Anaheim, CA (October 25- 30, 1992), Soc. Neurosci. Abstracts 18: 163, 1992.

23. Tirumalai, P. S. and Howells, R. D. Regulation of glutamate R3 receptor mRNA in rat cerebellum in response to acute and chronic morphine. Presented at College of Problems of Drug Dependence, Toronto (June 12-17, 1993).

24. Saha, D. P., Tirumalai, P. S., Scala, L. A. and Howells, R. D. Human ribosomal protein L37a: Sequence of the cDNA and predicted protein, analysis of gene expression and translational regulation. Presented at the annual meeting of the American Society of Biochemistry and Molecular Biology, May 21-25, 1994.

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25. Scala, L. A., Tirumalai, P. S., Piparo, K. E. and Howells, R. D. Molecular cloning of a human homolog of Drosophila enhancer of split. Presented at the annual meeting of the American Society of Biochemistry and Molecular Biology, May 21-25, 1994.

26. Wang, W. W. and Howells, R. D. Cloning and sequencing of rat c-fos gene. Presented at the annual meeting of the American Society of Biochemistry and Molecular Biology, May 21-25, 1994.

27. Shahrestanifar, M., Saha, D. P., Basu, A., Scala, L. A. and Howells, R. D. Molecular cloning of a novel human nucleotide-binding protein. Presented at the annual meeting of the American Society of Biochemistry and Molecular Biology, May 21-25, 1994.

28. Shahrestanifar, M. and Howells, R. D. Sensitivity of and opioid receptor binding to N-ethylmaleimide. Presented at International Narcotic Research Conference, N. Falmouth, MA, July 16-21, 1994.

29. Shahrestanifar, M. and Howells, R. D. Characterization of mu and delta opioid receptor binding domains: sensitivity to N-ethylmaleimide. Presented at the 24th annual meeting of the Society for Neuroscience, Miami Beach, FL, (November 13-18, 1994). Soc. Neurosci. Abstracts 20: 1731, 1994.

30. Wang, W. W., Shahrestanifar, M., Ledeen, R. W. and Howells, R. D. Identification of type-specific ligand binding domains in the cloned mu and delta opioid receptors. Soc. Neurosci. Abstracts 21: 219.7, p. 526, 1995.

31. Shahrestanifar, M., Wang, W. W. and Howells, R. D. Role of reduced and oxidized cysteines in mu and delta opioid receptor binding. Soc. Neurosci. Abstracts 21: 219.6, p.526, 1995.

32. Shahrestanifar, M. and Howells, R. D. Sensitivity of opioid receptor binding to N-substituted maleimides and derivatives of methanethiosulfonate. 27th International Narcotic Research Conference, Long Beach, CA, July 22, 1996.

33. Wu, G., Dondio, G., Raveglia, L. F. and Howells, R. D. Opioid ligand/receptor interactions as revealed from analysis of the pH dependence of binding. Soc. Neurosci. Abstracts 23: 479.8, p. 1206, 1997.

34. Wu, G., Howells, R. D., Christoffers, K., Lu, Z.-H., and Ledeen, R. W. Interaction of the delta-opioid receptor with GM1 ganglioside: conversion from inhibitory to excitatory

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mode. Sphingolipids as Signaling Modulators in the Nervous System, pg. 23, Satellite Symp. to Int. and Am. Soc. Neurochem., New York City, July 13-16, 1997.

35. Howells, R. D., Chinen, N., Wu, G., Bandari, P. and Chaturvedi, K. Opioid receptor down-regulation: G protein coupling and proteases. 29th International Narcotic Research Conference, Garmisch-Partenkirchen, Germany, July 20-25, 1998.

36. Howells, R. D., Edwards, E., Wu, G., Chaturvedi, K. and Dondio, G. Pharmacological profile of non-peptide delta opioid receptor ligands. 29th International Narcotic Research Conference, Garmisch-Partenkirchen, Germany, July 20-25, 1998.

37. Howells, R.D., Chaturvedi, K., Bandari, P. and Chinen, N. Degradation of opioid receptors by the proteasome. 2nd European Opioid Conference, Dolor 14 (Supplement 1): 24, 1999.

40. Howells, R.D., Chaturvedi, K., Bandari, P. and Chinen, N. The proteasome and opioid receptor down-regulation. 30th International Narcotic Research Conference, Saratoga, New York, July 10-15, 1999.

41. Chaturvedi, K., Shahrestanifar, M. and Howells, R.D. Opioid receptor: role of the amino terminus in ligand binding affinity. Soc. Neurosci. Abstracts, 25, 680.13, p. 1707, 1999.

42. Christoffers, K., Wu, G., Howells, R.D., Lu, Z.-H. and Ledeen, R.W. Interaction of delta opioid receptor with GM1 ganglioside: conversion from inhibitory to excitatory mode. International Conference on Neurochemistry, Berlin, Germany, Aug. 9-14, 1999.

43. Howells, R.D., Chaturvedi, K., Bandari, P., Jiang, X. and Chinen, N. The proteasome and agonist-induced opioid receptor down-regulation. Minisymposium '99: "From Molecules to Cognition", Rutgers' Center for Molecular and Behavioral Neuroscience, Newark, NJ Oct. 19, 1999.

44. Chaturvedi, K., Bandari, P. and Howells, R.D. Differential sensitivity of mu and delta opioid receptor down regulation to tyrosine kinase inhibitors. Soc. Neurosci. Abstracts, 26. 783.9, p. 2098, 2000.

45. Khohkar, A., Chaturvedi, K. and Howells, R.D. Inhibition of opioid receptor binding by the protease inhibitor, leupeptin. 2000 GSBS Summer Research Experience for Undergraduates Program, Aug. 3, 2000.

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46. Howells, R.D., Chaturvedi, K., Bandari, P., and Chinen, N. Proteasome involvement in agonist-induced down regulation of mu and delta opioid receptors. Winter Brain Conference, Steamboat Springs, CO, Jan. 20-27, 2001.

47. Keenan, S.M., Howells, R.D., Peng, Y., Yoon, S.-J., Zhang, Q. and Welsh, W.J. Computer-aided molecular design, synthesis and pharmacological evaluation of a novel family of delta-selective opioid receptor ligands, International Narcotic Research Conference, Perpignan, France, July 6-11, 2003.

48. Christoffers, K.H., Li, H. and Howells, R.D., Purification and mass spectrometry of the delta opioid receptor, International Narcotic Research Conference, Perpignan, France, July 6-11, 2003.

49. Christoffers, K.H., Li, H. and Howells, R.D., Purification and mass spectrometry of the mu opioid receptor, International Narcotic Research Conference, Perpignan, France, July 6-11, 2003.

50. Wannemacher, K.M., Christoffers, K.H. and Howells, R.D., Heat shock protein Hsp70 associates with mu and delta opioid receptors. Experimental Biology 2004, Washington, D.C., April 17-21, 2004.

51. Yadav, P. and Howells, R.D., Inhibition of agonist-induced down regulation of the delta opioid receptor attenuates the development of opioid tolerance. Society for Neuroscience, San Diego, CA, October 23-27, 2004.

52. Wannemacher, K., Yadav, P.N., Doligosa, M. and Howells, R.D., Naltrexone up-regulates delta opioid receptor binding without increasing mature receptor protein. International Narcotic Research Conference, Annapolis, MD, July 10-15, 2005.

53. Yadav, P.N., Wannemacher, K.M., Balan, N. and Howells, R.D., The histone deacetylase inhibitor, trichostatin A, stimulates expression of the delta opioid receptor in HEK 293 cells. International Narcotic Research Conference, Annapolis, MD, July 10-15, 2005.

54. Wannemacher, K.M., Yadav, P.N. and Howells, R.D., Antagonist-induced opioid receptor up-regulation. Society for Neuroscience, Washington DC, November, 2005.

55. Wannemacher, K.M., Yadav, P.N. and Howells, R.D., Opioid antagonists up-regulate delta opioid receptor binding without increasing immunoreactive receptor protein. International Narcotic Research Conference, St. Paul, MN, July 9-14, 2006.

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56. Yadav, P.N., Wannemacher, K.M. and Howells, R.D., Interaction of heat shock protein 70 with the delta opioid receptor. International Narcotic Research Conference, St. Paul, MN, July 9-14, 2006.

57. Wannemacher, K.M., Yadav, P.N. and Howells, R.D., Antagonist-induced kappa opioid receptor up-regulation. Society for Neuroscience, Atlanta, GA, November, 2006.

58. Wannemacher, K.M., Terskiy, A., Bian, S., Yadav, P.N., Li, H. and Howells, R.D., Purification and mass spectrometric analysis of the kappa opioid recepor. Society for Neuroscience, San Diego, CA, November, 2007.

59. Terskiy, A., Wannemacher, K.M. and Howells, R.D., Inhibition of human multiple myeloma cells by select opioids. American Association for Cancer Research, San Diego, CA, April, 2008.

60. Terskiy, A., Wannemacher, K.M. and Howells, R.D., Inhibition of proliferation of multiple myeloma cells by naltrindole. International Narcotic Research Conference, Charleston, SC, July 2008.

61. Wannemacher, K.M. and Howells, R.D., Mechanisms of antagonist-mediated delta opioid receptor upregulation. International Narcotic Research Conference, Charleston, SC, July 2008.

62. Howells, R.D., Yadav, P.N., Wannemacher, K.M., Yadav, P.N. and Terskiy, A., Differential regulation of delta opioid receptor expression by histone deacetylase inhibitors, Society for Neuroscience, Washington DC, November 2008.

63. Howells, R.D., Howells, A.R. and Comollo, T.W., Involvement of lysine residues on the exoplasmic surface of the delta opioid receptor in ligand binding and receptor folding. Society for Neuroscience, Chicago, Il, October 2009.

64. Joshi, J., Terskiy, A. and Howells, R.D., Inhibition of human multiple myeloma cell proliferation by naltrindole. American Association for Cancer Research, Orlando, FL, April 2011.

65. Joshi, J., Terskiy, A. and Howells, R.D., Inhibition of human multiple myeloma cell proliferation by naltrindole. International Narcotic Research Conference, Hollywood, FL, June 2011.

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66. Howells, R.D., Mundra, J.J., Howells, A.R., Howells, G.D., Peng, Y., Welsh, W.J., Novel small-molecule delta opioid receptor ligands modeled from naltrindole inhibit the proliferation of human U266 multiple myeloma cells. American Association for Cancer Research, Washington, DC, April 2013.

67. Avrutik, J.F., Mundra, J.J., Peng, Y., Welsh, W.J. and Howells, R.D. Molecular modeling of naltrindole led to the discovery of novel triazole-based inhibitors of the proliferation of human multiple myeloma cells. Cancer Institute of New Jersey, Piscataway, NJ, May 2013.

68. Howells, R.D., Mundra, J.J., Howells, A.R., Howells, G.D., Peng, Y., Welsh, W.J., Novel small-molecule delta opioid receptor ligands modeled from naltrindole inhibit the proliferation of human U266 multiple myeloma cells. Presented at the Faculty Poster Day, Rutgers Graduate School of Biomedical Sciences, September 2013.

69. Welsh, W.J., Howells, R.D., Peng, Y., Mundra, J.J., Howells, A.R. and Howells, G.D. Discovery of novel triazole-based inhibitors of the proliferation of human multiple myeloma cells based on naltrindole. American Association of Pharmaceutical Scientists, San Antonio, TX, November 2013.

70. W.J. Welsh, Y. Peng, J. J. Mundra, J.F. Avrutik, T.C. Yoon, A.M. Meillier and R.D. Howells. Novel trisubstituted triazoles modeled from naltrindole inhibit the proliferation of human multiple myeloma cells. American Association for Cancer Research, San Diego, CA, April 2014; Cancer Res 2014;74(19 Suppl):Abstract nr 1626. doi:10.1158/1538-7445.AM2014-1626.

71. R.D. Howells, T.C. Yoon, A.M. Meillier, Y. Peng, and W.J. Welsh. Novel small molecule triazoles modeled from naltrindole potently inhibit the proliferation of human multiple myeloma cells. Cancer Institute of New Jersey Annual Retreat, Piscataway, NJ May, 2014.

72. R.D. Howells, T.C. Yoon, A.M. Meillier, Y. Peng, and W.J. Welsh. Novel small molecule triazoles modeled from naltrindole potently inhibit the proliferation of human multiple myeloma cells. International Narcotic Research Conference, Montreal, Canada July 2014.

23. REVIEWS

1. Chaturvedi, K., Christoffers, K., Singh, K. and Howells, R.D. Down Regulation of Opioid Receptors, Biopolymers/Peptide Science, 55 (4): 334-346, 2000.

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24. REPORTS - None

25. COMMUNITY SERVICE2001-2004 Randolph Township NJ Little League Baseball Coach2001-2002 Randolph Township NJ Recreational Youth Soccer Coach2006 Guest musician for Shongum Elementary School production,

Randolph NJ2006-2007 Randolph Township NJ Recreational Youth Basketball Coach2009-2012 Volunteer, Randolph High School Boys Soccer 2010-present Advocated for increases in Federal funding to NIH through

correspondence with President Obama, Senators Robert Menendez and Frank Lautenberg (and subsequently Corey Booker), Representatives Donald Payne, Rodney Frelinghuysen via ASPET, Society of Neuroscience

2013-present Volunteer, Drew University Varsity Men’s Soccer Team

APPENDIX

MANUSCRIPTS SUBMITTED/IN PREPARATION

Joshi, J., Gaspers, L, Thomas, A. and Howells, R.D. Non-opioid receptor-dependent effects of naltrindole on calcium signaling in human multiple myeloma cells. Submitted to the Journal of Pharmacology and Experimental Therapeutics. Currently under revision.

Howells, R.D. Naltrindole inhibits human multiple myeloma tumor growth in a mouse xenograft model in vivo with an efficacy similar to bortezomib.

Avrutik, J.F., Mundra, J.J., Peng, Y., Welsh, W.J. and Howells, R.D. Molecular modeling of naltrindole led to the discovery of novel triazole-based inhibitors of the proliferation of human multiple myeloma cells.

Wannemacher, K.M. and Howells, R.D. Opioid antagonists induce proper folding of δ-opioid receptors resulting in increased ligand binding without increasing receptor protein levels.

Wannemacher, K.M., Yadav, P.N., Terskiy, A., and Howells, R.D. Regulation of delta opioid receptor gene expression in response to inhibition of histone deacetylase.

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Yadav, P.N., Wannemacher, K.N. and Howells, R.D., Interaction of heat shock protein 70 with the delta opioid receptor.

Khariv, V., Joshi, J. and Howells, R.D. Naltrindole inhibits the proliferation of human melanoma cells in vitro.

Joshi, J. and Howells, R.D. Naltrindole inhibits the proliferation of human MCF-7 breast cancer cells in vitro.

Mundra, J.J., Kuzhikandathil, E. and Howells, R.D. Naltrindole inhibits sodium and potassium ion currents and the proliferation of mouse AtT-20 cells.

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