separates substances w/in a mixture based on their physical properties used to: - analyze dyes in...
DESCRIPTION
Paper Chromatography – small amount of substance placed near bottom of paper, bottom placed in solvent - solvent moves up paper = mobile phase - paper itself = stationary phase - As solvent moves, different components of mixture adhere to paper at different placesTRANSCRIPT
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• Separates substances w/in a mixture based on their physical properties• Used to: - analyze dyes in fibers - test for explosives or accelerants - check body fluids for drugs
![Page 3: Separates substances w/in a mixture based on their physical properties Used to: - analyze dyes in fibers - test for explosives or accelerants - check](https://reader036.vdocuments.us/reader036/viewer/2022082723/5a4d1ad47f8b9ab0599726c6/html5/thumbnails/3.jpg)
• Paper Chromatography – small amount of substance placed near
bottom of paper, bottom placed in solvent- solvent moves up paper = mobile phase- paper itself = stationary phase
- As solvent moves, different components of mixture adhere to paper at different places
![Page 4: Separates substances w/in a mixture based on their physical properties Used to: - analyze dyes in fibers - test for explosives or accelerants - check](https://reader036.vdocuments.us/reader036/viewer/2022082723/5a4d1ad47f8b9ab0599726c6/html5/thumbnails/4.jpg)
• Chromatogram – shows substances dissolved in original mixture & how far solvent traveled
![Page 5: Separates substances w/in a mixture based on their physical properties Used to: - analyze dyes in fibers - test for explosives or accelerants - check](https://reader036.vdocuments.us/reader036/viewer/2022082723/5a4d1ad47f8b9ab0599726c6/html5/thumbnails/5.jpg)
• Rf value = retention factor - qualitative comparison b/t length of
time substance is in mobile & stationary phases
Rf = distance substance traveled distance solvent traveled - depends on type of solvent - solvent front = line where solvent stopped
moving
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• Thin Layer Chromatography (TLC) - similar to paper, but faster &
clearer - use thin layer of gel-like material
on a glass or plastic plate - good to separate dyes & inks
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• Gas Chromatography (GC) - at high temps - useful to separate mixtures w/
large molecules (ex. Proteins in blood)
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• High Performance Liquid Chromatography (HPLC)
- at room temp - uses high pressure to force
mixtures through a column of liquid