Do you need an extended temperature range to analyze high

molecular weight analytes?

Keep up the good work.

Keep up the good work.DB-HeavyWAX DB-WAX

Ultra Inert

DB-HeavyWAX for high molecular weight analytes

– Higher max temp limit: 280 °C isothermal and 290 °C programmed

– Shorter run times

www.agilent.com/chem/db-heavywax

DB-WAX Ultra Inert for polar compounds

– Withstands repeated temperature cycling to upper limits without sacrificing peak shape performance

– Identical selectivity to DB-WAX

www.agilent.com/chem/dbwaxui

DB-FATWAX Ultra Inert for FAMEs and free fatty acids

– Single column analysis

– Solvent rinseable and tolerates aqueous injections

www.agilent.com/chem/fatwax-ui

2x10

- 0.1- 0.05

00.05

0.10.15

0.20.25

0.30.35

0.40.45

0.50.55

0.60.65

0.70.75

0.80.85

0.90.95

11.05

2x10

- 0.1- 0.05

00.05

0.10.15

0.20.25

0.30.35

0.40.45

0.50.55

0.60.65

0.70.75

0.80.85

0.90.95

11.05

Counts (%) vs. Acquisition Time (min)

3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57 58 59 60 61 62 63 64 65 66 67 68 69

Acquisition Time (min)1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20

C9

C10C18C16

C14C12

C20 C22 C24C26

C28

C30C32

C34 C36

C9

C10

C18C16C14

C12C20 C22 C24C26 C28

C30C32

C34 C36

Final temp 280 °C

Final temp 250 °C1

2, 3

4

5

6 78

9

2 3 4 5 61

2 3 4 5 61

1

2

34

56 7

8

9

Competitor WAX column30m x 0.25 mm, 0.25 μm

1

2, 3

4

5

6 78

9

2 3 4 5 61

2 3 4 5 61

1

2

34

56 7

8

9

Agilent DB-FATWAX Ultra Inert 30m x 0.25 mm, 0.25 μm

Analysis of Cold Pressed Pink Grapefruit Essential Oil

1. Acetic acid2. Propionic acid3. Isobutyric acid4. Butyric acid5. Isovaleric acid6. Valeric acid7. 4-Methylvaleric acid8. Hexanoic acid9. Heptanoic acid

2x10

- 0.1- 0.05

00.05

0.10.15

0.20.25

0.30.35

0.40.45

0.50.55

0.60.65

0.70.75

0.80.85

0.90.95

11.05

2x10

- 0.1- 0.05

00.05

0.10.15

0.20.25

0.30.35

0.40.45

0.50.55

0.60.65

0.70.75

0.80.85

0.90.95

11.05

Counts (%) vs. Acquisition Time (min)

3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57 58 59 60 61 62 63 64 65 66 67 68 69

Acquisition Time (min)1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20

C9

C10C18C16

C14C12

C20 C22 C24C26

C28

C30C32

C34 C36

C9

C10

C18C16C14

C12C20 C22 C24C26 C28

C30C32

C34 C36

DB-HeavyWAXRun time: 13 minFinal temp: 280 °C Ramp: 30 °C/min Flow: 5 mL/min

DB-WaxRun time: 19 minFinal temp: 250 °C Ramp: 30 °C/min Flow: 5 mL/min

Superior inertness, unique selectivity, and thermal robustness for separation of FAMEs and underivatized free fatty acids.

High temperature capability of Agilent J&W DB-HeavyWAX enables shorter run times and sharper peaks for high molecular weight, high boiling compounds without the risk of carry-over.

This information is subject to change without notice.

© Agilent Technologies, Inc. 2018 Published in the USA, June 29, 2018 5991-9415EN

Is this a new or existing method?

Existing New

Yes

Yes Yes

No FAMEs and/or free fatty acids

NoNo

DB-FATWAX Ultra Inert

VF-WAX MS

DB-WAX Ultra InertDB-HeavyWAX

All other analytes*

All other detectors

Mass spectrometry

What is your maximum operating temperature?

> 250 °C < 250 °C

Even after 50 hours of exposure at the upper temperature range, the DB-WAX UI GC column demonstrated superior inertness and stability with minimal peak shifting.

2.98

pAXOf fset : 0 YOf fset : 22

28272625242322212019181716151413121110987654321

5048464442403836343230282624222018161412108642

DB-WAX UI after 50h at 250 ºCDB-WAX UI after 1h at 250 ºC

1

23.93

34.28

45.74

57.36

69.29

711.16

813.25

914.55

1016.54

1120.69

1225.35

1.46

1

23.96

34.33

45.85

57.39

69.48

711.27

813.52

914.73

1016.59

1120.99

1225.88

1.46

RT [min]

Inertness of Agilent DB-WAX UI

272625242322212019181716151413121110987654321

525048464442403836343230282624222018161412108642

1.34

2.59

RT [min]

pA

23.41

34.33

44.89

56.30

68.09

79.44

811.79

912.08

1014.07

1119.37 12

22.73

1

XOffset : 0 YOffset : 23

Competitor Standard WAX after 50h at 250 ºCCompetitor Standard WAX after 1h at 250 ºC

1

23.42

34.04

44.94

56.29

79.45

811.91

912.12

1014.02

1118.55 12

22.14

68.17

Inertness of competitor WAX column

Injector: 250 °C, Split 1:75Injection volume: 1 µLFlow rate: 1.1 mL/min, H2 gas Oven program: 130 °C Isothermal Detector: FID @ 260 °C

1. 2-Nonanone2. Decanal3. Propionic acid4. Ethylene glycol5. Heptadecane6. Aniline7. Methyl dodecanoate8. 2-Chlorophenol9. 1-Undecanol10. Nonadecane11. 2-Ethylhexanoic acid12. Ethyl maltol

Are you satisfied with the sensitivity and reproducibility of your analysis and frequency of column changes?

Are you using DB-WAX Ultra Inert? What compounds are you analyzing?

What detector are you using?

42 42.5 43 43.5 44 44.5 45 45.5 46 46.5 47 47.5 48 48.5 49 49.5 50 50.5 51 51.5 52 52.5 53 53.5 54 54.5 55 55.5 56 56.5 57 57.5 58 58.5 59 59.5 60 60.5 61 61.5 62 62.5 63 63.5 64 64.5 65

Initial Injection

Repeat Injection

Meranzin

Isomeranzin

Osthole

*Specialty columnsStrong acids DB-FFAPBasic analytes and amines CAMAlcohols and glycols in wine/spirits CP-WAX 57CB

Selecting the Right WAX ColumnAgilent J&W WAX GC columns deliver excellent inertness with greater sensitivity than competitive WAX columns. With the increasing number of available Agilent J&W WAX columns, it can be challenging deciding which one is best suited for your needs.

This poster will help you select the right WAX column for optimal performance, based on factors such as application analyte mix and the type of detector being used.