sealer ppt

8/11/2019 Sealer Ppt

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OBJECTIVES

To evaluate the cytotoxicity and genotoxicity inducedby root canal sealers-

1. Endoflas (Control group)

2. MTA fillapex,3. Rokeoseal,

4. Diapex and Experimental groups

5. R-C seal

on Human Embryonic Kidney-293 cell cultures by MTTassay and MNT assay.

To compare the biocompatibility of the above groups


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EXPERIMENTAL DESIGNING

IN VITROHEK-293 cells(n=40)

Endoflas MTA filapex Rokeoseal Diapex RC seal

(n=8) (n=8) (n=8) (n=8) (n=8)

Cytotoxicity Genotoxicity cytotoxicity genotoxicity cytotoxicity genotoxicity cytotoxicity genotoxicity cytotoxicity genoyoxi

.

MTT MNT MTT MNT MTT MNT MTT MNT MTT MNT


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STATISTICAL ANALYSIS


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APPLICATION

Biocompatibility of dental materials is of great importanceas cytotoxic materials may cause inflammatory reactionswhen in direct contact with adjacent tissues, compromisingthe treatment outcome.

Endodontic therapy aims at elimination of residual pulp,tissue breakdown products, microorganisms present insidethe root canal system, followed by hermetic filling aspossible, perfect apical seal, tissue mineralizationinduction, immunological compatibility, antimicrobialactivity.

In addition Grossman recommended endodontic sealersnot to provoke an immune response in periradicular tissueand neither be mutagenic nor carcinogenic


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STATEMENT OF PROBLEM

Root canal sealers are routinely used in

conventional root canal therapy.

Some endodontic materials have been shown

to have a mutagenic potential to the

surrounding periradicular tissues.


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SIGNIFICANCE OF THE PROBLEM

Adverse material effects may play an important role in

the failure of endodontic treatment, even if no majorfault can be identified in treatment.

Therefore, only those root canal sealers that arebiocompatible and display no mutagenic potential

should be used in conventional root canal therapy. This demands that each endodontic material should be

evaluated for its mutagenic potential before clinicalapplication.

For example,sealers with inferior biocompatibility, suchshould no longer be applied during treatment


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METHODOLOGY

CELL LINE MAINTENANCE

(Cells will be grown &maintained in DMEM medium supplement with 10% FBS & 1% antibiotic

solution at 370C with 5% CO2 in a humidified atmosphere)

CELL LINE HARVESTING/ SUB-CULTURING OF CELL

(Cells will be trypsinized by using 0.25% trypsin-EDTA solution )

CRYOPRESERVATION OF CELL LINE

REVIVING OF FROZEN CELL


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Extract/Sample preparations(All the five endodontic sealers included in this study will be mixed according to

manufacturers instructions under aseptic environment. The materials will be then

placed in non-reactive 12 well plates and allowed to set according to themanufacturers instructions at 37C)

FRESH EXTRACT

24 HOUR EXTRACT

7 DAYS EXTRACT

Will be collected.All extracts will be stored at -20C till further use.

Cytotoxicity assay of root canal sealer extracts

MTT ASSAY FOR DETERMINING CELL GROWTH INHIBITION

Data Interpretation of MTT assay


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Genotoxicity assay of root canal sealer extracts

MNT ASSAY

Data Interpretation of MNT assay

STATISTICAL ANALYSIS

sealer ppt

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