scanning em of c. elegans

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Scanning em of C. elegans

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Scanning em of C. elegans. Crawl movie. Fly-in movie. Male, scanning em. Male tail scanning em. Rene Mating Movie. Embryonic cell lineage. D lineage. ABala lineage. AIML lineage. AIML neuron. AIML circuit. Postembryonic blast cells. Z1Z2 Z3 Z4. Adult hermaphrodite anatomy. - PowerPoint PPT Presentation

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Page 1: Scanning em of  C. elegans

Scanning em of C. elegans

Page 2: Scanning em of  C. elegans

Crawl movie

Page 3: Scanning em of  C. elegans

Fly-in movie

Page 4: Scanning em of  C. elegans

Male, scanning em

Page 5: Scanning em of  C. elegans

Male tail scanning em

Page 6: Scanning em of  C. elegans

Rene Mating Movie

Page 7: Scanning em of  C. elegans

Embryonic cell lineage

Page 8: Scanning em of  C. elegans

D lineage

Page 9: Scanning em of  C. elegans

ABala lineage

Page 10: Scanning em of  C. elegans

AIML neuron

AIML lineage

Page 11: Scanning em of  C. elegans

AIML circuit

Page 12: Scanning em of  C. elegans

Z1Z2 Z3 Z4

Postembryonic blast cells

Page 13: Scanning em of  C. elegans

Adult hermaphrodite anatomy

Page 14: Scanning em of  C. elegans

C. elegans typical cross section

Page 15: Scanning em of  C. elegans

Seam cell postembryonic lineages in the male

wild type

lin-4(e912) reiterations

Page 16: Scanning em of  C. elegans

wild type

lin-14(sd) repeats S1

lin-14(lf) skips S1

Page 17: Scanning em of  C. elegans

lin-4(e912) lin-14(sd)

lin-4(lf) and lin-14(gf) have the same phenotype: they repeat S1.

Page 18: Scanning em of  C. elegans

LIN-4S is complementary to sequences in the 3’UTR of lin14.

Page 19: Scanning em of  C. elegans

Human miRNA genes

Page 20: Scanning em of  C. elegans

Symmetric neuron phenotype of lsy-6(-)

Page 21: Scanning em of  C. elegans

A regulatory pathway leading to neuron asymmetry involving two micro RNA’s

Page 22: Scanning em of  C. elegans

Worms Eating Bacteria Expressing dsRNA to a GFP Reporter Gene Activate RNAi

wild type worms RNAi-defective mutants

GFP lost in embryosGFP lost in the gut

from Mello and Conte,Nature 431, 338-342.

Page 23: Scanning em of  C. elegans

RNAi in Plants

1928 report of developmentof viral (tobacco ringspot virus)resistance in tobacco, nowknown to be due to endogenousRNAi.

1928 report of developmentof viral (tobacco ringspot virus)resistance in tobacco, nowknown to be due to endogenousRNAi.

Page 24: Scanning em of  C. elegans

RNAi/miRNA Pathways

from Meister and Tuschl, Nature431, 343-349, 2004.

Page 25: Scanning em of  C. elegans

The Structure of Argonaute (Slicer)

Song et al., Science305, 1434-1437, 2004.