scale up in bioreactor
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8/7/2019 Scale Up in bioreactor
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Er.P.V.S.Yugandhar
SCALESCALE--UPUP
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Er.P.V.S.Yugandhar
ScaleScale--up is the complex of up is the complex of techniques and methodologiestechniques and methodologies
employed to transfer to theemployed to transfer to the
production scale aproduction scale a
fermentation processfermentation process
developed on a smaller scale.developed on a smaller scale.
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Not necessarily a one-way procedure---
from small to large In practice the complex nature of the
processes involved requires a scale-
down operation to obtain an additionalinformation
Scale-down is a basic part of any scale-
up procedure.
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Scale-up is a highly interdisciplinary
task , requiring the combined andintegrated use of concepts and
methodologies of both chemical
engineering and living cell physiology
There is no unique and universal scale-
up procedure, as each process is likelyto present its own peculiarities and
difficulties
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Effects of Fermentation ScaleEffects of Fermentation Scale
Most results of scale have been
obtained with series of geometrically similar fermenters---
---vessels whose linear dimensions
stand in the same ratios to eachother on the different scales
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Parameters affected by scaleParameters affected by scale
Number of generations
Development of proceduresSterilization of culture medium
Aeration and agitationHeat transfer
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Number of generationsN
umber of generations The number of generations required to
achieve a given final biomass concentration
x is obviously a function of fermenter
volume.