sc-rtta with mutations in the n-terminal tetr domain

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0.1 1 10 100 1000 10000 - w ild-type F86Y V9I F86Y F67S F86Y F86Y G 138D V9I F86Y G 138D rtTA 0.1 1 10 100 1000 10000 - w ild-type F86Y V9I F86Y F67S F86Y F86Y G 138D V9I F86Y G 138D rtTA 0.1 1 10 100 1000 10000 - w ild-type F86Y V9I F86Y F67S F86Y F86Y G 138D V9I F86Y G 138D rtTA 0 10 50 100 500 1000 dox (ng/m l) sc-rtTA with mutations in the N-terminal TetR domain A Transcriptional activity (%) sc-rtTA with mutations in the C-terminal TetR domain B Transcriptional activity (%) sc-rtTA with mutations in both TetR domains C Transcriptional activity (%) rtTA - wild-type V9I F86Y F67S F86Y F86Y V9I F86Y G138D F86Y G138D rtTA - wild-type V9I F86Y F67S F86Y F86Y V9I F86Y G138D F86Y G138D rtTA - wild-type V9I F86Y F67S F86Y F86Y V9I F86Y G138D F86Y G138D

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A. -. -. -. wild-type. wild-type. wild-type. V9I F86Y. V9I F86Y. V9I F86Y. F67S F86Y. F67S F86Y. F67S F86Y. F86Y G138D. F86Y G138D. F86Y G138D. V9I F86Y G138D. V9I F86Y G138D. V9I F86Y G138D. rtTA. rtTA. rtTA. F86Y. F86Y. F86Y. - PowerPoint PPT Presentation

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Page 1: sc-rtTA with mutations in the N-terminal TetR domain

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- w ild-type F86Y V9I F86Y F67S F86Y F86YG138D

V9I F86YG138D

rtTA

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- w ild-type F86Y V9I F86Y F67S F86Y F86YG138D

V9I F86YG138D

rtTA

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10000

- w ild-type F86Y V9I F86Y F67S F86Y F86YG138D

V9I F86YG138D

rtTA

010501005001000

dox (ng/ml)

sc-rtTA with mutations in the N-terminal TetR domainA

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sc-rtTA with mutations in the C-terminal TetR domainB

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sc-rtTA with mutations in both TetR domainsC

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rtTA- wild-type V9I F86Y F67S F86YF86Y V9I F86YG138D

F86YG138D

rtTA- wild-type V9I F86Y F67S F86YF86Y V9I F86YG138D

F86YG138D

rtTA- wild-type V9I F86Y F67S F86YF86Y V9I F86YG138D

F86YG138D

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