sap2143- tissueculture course outline

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INTERNATIONAL ISLAMIC UNIVERSITY MALAYSIA

COURSE OUTLINE

Kulliyyah Science

Department Plant Sciences

Programme Bachelor of Applied Plant Sciences (Hons)

Name of Course / Model Plant Tissue Culture

Course Code SAP 2143

Name (s) of Academic staff /

Instructor(s)

Dr. Zarina Zainuddin

Tel. No.: 09-5705003

Email: [email protected]

Rationale for the inclusion

of the course / module in the

programme

This is a core/required course for the Bachelor degree in Plant Sciences

Semester and Year Offered Semester I, Second Year

Status Core Course

Level 2

Proposed Start Date September 2013

Batch of Student to be

Affected Starts with Batch 2013/2014

Total Student Learning

Time (SLT)

Contact Learning Time Independent

Learning Activities

Formal

assessment T

ota

l S

LT

Teacher-

centered

Student-

centered

learning

No

n f

ace-

to-f

ace

lear

nin

g

(e.g

. :

assi

gn

men

t, m

odu

le,

pro

ject

)

Rev

isio

n

Pre

par

atio

n f

or

asse

ssm

ent

Co

nti

nu

ou

s as

sess

men

t

Fin

al e

xam

Lec

ture

Pra

ctic

al/t

uto

rial

Co

nsu

ltat

ion

/PB

L/S

emin

ar

42 0 0 8 42 22 2 3 119

Conversion of SLT into tentative credit (TOTAL 40) 2.98

Credit to be assigned to the course 3

of 6

Credit Value / Hours 3

Pre-requisites (if any) Nil

Co-requisites (if any) Nil

Course Objectives

The objectives of the course are to:

1. Provide the basic knowledge of plant cell and tissue culture 2. Give examples of various basic techniques and protocols

employed in plant tissue culture

3. Acquaint students with the principles, technical requirement, scientific and commercial applications of plant tissue and cell

culture

Learning Outcomes

Upon completion of this course, students should be able to:

1. Identify the principles related to plant tissue culture (P1, A2, LL2)

2. Explain the different basic techniques and approaches used in plant tissue culture research (C1, P5)

3. Conduct laboratory procedures for plant propagation using plant tissue culture techniques .(C1)

4. Describe different applications of plant tissue culture especially in plant biotechnology

Transferable Skills:

Skills and how they are developed and assessed, project and practical

experience and internship

Skill Assessment How skills are

developed

Exam

Qu

iz

Tu

toria

l

Ass

ign

men

t

Pre

sen

tati

on

Communication

Oral presentation,

Group Discussion,

Short answer

Critical Thinking /

Problem solving

Group discussion,

Tutorials

Creativity / Inovation

Oral presentation,

Tutorials

ICT / Information

Management

Collecting data and

information for report

Teamwork

Group presentation,

Laboratory

Self-awareness

Short answer during

QA.

Teaching-Learning and

assessment strategy

No. Teaching-Learning Assessment

1 Lecture Exam, Quiz, Assignment

2 Tutorial Exam, Quiz, Q&A

Course Synopsis This course is intended to give an introduction to plant tissue culture.

The course will provide an overview of principles, techniques, and

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applications of plant tissue culture.Protocols that are routine and

approaches associated withthe culture of plant cells in the laboratory

will also be discussed in detail. The applications of plant tissue in

genetic engineering, genetic transformation and developing transgenic

plants will be detailed out.

Mode of Delivery Lectures/Tutorials, Quizzes, Assignments/Presentation

Assessment Methods and

Type / Course Assessement

State weightage of each type

of assessment.

LO Method %

1, 2 Assignments 5

Lab reports 15

1, 2, 3 Quizzes 10

1, 2, 3 Mid-term Examination 25

1, 2, 3, 4 Final Examination 45

Total 100

Mapping of the course /

module to the programme

aims

Course Learning Outcome

Programme aims

/ objectives

Aim

1

Aim

2

Aim

3

Aim

4

Aim

5


2. Explain the different basic techniques and approaches used in plant tissue culture research

(C1, P5)

3. Conduct laboratory procedures for plant propagation using plant tissue culture

techniques .(C1)


Mapping of course / module to the Programme Learning Outcomes

Learning Outcome of the course

Programme Outcomes

PO

1

PO

2

PO

3

PO

4

PO

5

PO

6

PO

7

PO

8

PO

9

PO

10


2. Explain the different basic techniques and approaches used in plant tissue culture research (C1, P5)

3. Conduct laboratory procedures for plant propagation using plant tissue culture techniques .(C1)


Content outline of the course / module and the SLT per topic

Weeks Topics Learning

Hours Task/Reading

1 History of plant cell culture

Introduction. The early years. The era of techniques

development. The recent past. The present era.

3 Smith (2000)

Chapter 1

of 6

2 Laboratory design and development

Structures and facilities. Media preparation area. Washing and

sterilization area. Inoculation area/sterile transfer area. Culture

room/growth room/incubation room. General storage area.

Research area. Planting area.

6.5

Sathyanarayana

(2007)

Chapter 2

3 Instrumentation

Laminar flow hoods. Autoclaves. Distillation unit. pH meter.

Orbital shaker. Other instruments.

7.25

Sathyanarayana

(2007)

Chapter 3

4 Plant tissue culture media

Solid and liquid media. Inorganic nutrients: micronutrients

and macronutrients. Organic supplements: vitamins, amino

acids, growth regulators. Source of carbon and energy.

Solidifying agents. pH of media. Types of media.

7

Sathyanarayana

(2007)

Chapter 4

5 Sterilization techniques

Sterilization of culture vessels and instrumentations.

Sterilization of nutrient media. Sterilization of explants.

7.5

Sathyanarayana

(2007)

Chapter 5

6 Cellular totipotency

Vascular differentiation. Phloem differentiation. Organogenic

differentiation. Totipotency of epidermal cells. Totipotency of

crown gall cells.

5.25 Razdan (2003)

Chapter 6

7 Micropropagation

Methods of micropropagation. Advantages and disadvantages.

Commercial propagation.

Quiz

12

Jha&Gosha(200

5)

Chapter 4

8 Somatic embryogenesis Somatic embryogenesis in dicotyledonous cultures. Somatic

embryogenesis in monocotyledonous cultures. Embryo

maturation and plantlet development. Loss of morphogenic

potential in embryogenic cultures. Practical applications.

17.5 Razdan (2003)

Chapter 7

9 Anther pollen and ovary culture

Introduction. Factors to be considered for successful anther

culture. General anther culture procedure. In vitro pathways of

androgenesis. Application of haploids in agriculture.

7.25

Jha&Gosha

(2005)

Chapter 6

10 Protoplast culture and somatic hybridization

Introduction. Isolation and culture of plant protoplasts. Gene

transfer with the use of protoplast.

7 Jha (2005)

Chapter 5

11 Callus and suspension culture

Fermenter culture. Immobilized cell cultures. Nutrient media.

Evaluation of experiments. Maintenance of strains,

cryopreservation. Physiological, histological and biochemical

aspects. Methods to establish cell suspension. Cell population

dynamics.

7.5

Neumannet al.

(2009)

Chapters 3, 4

12 Somaclonal variation

Quiz

5.25

Jha&Gosha

(2005)

Chapter 7

13 Embryo and endosperm culture

5.5

Jha&Gosha

(2005)

Chapter 8

of 6

14 Applications of plant tissue culture Genetic engineering. Genetic transformation. Transgenics

Jha&Gosha

(2005)

Chapters 10, 11,

12

Main references supporting the course

The reference lists shall be presented in accordance with APA bibliographic practices and in

alphabetical order.

Required

1. Smith, R.H., (2000), Plant Tissue Culture: Techniques and experiments, Gulf Professional Publishing.

2. Sathyanarayana, B.N., (2007), Plant Tissue Culture: Practices and new experimental protocols, I. K. International Pvt. Ltd.

3. Razdan, M.K., (2003), Introduction to plant tissue culture, Science Publishers. 4. Jha, T.B. and Gosha, B., (2005), Plant tissue culture: Basic and applied, Universities Press. 5. Neumann, K-H., Kumar, A. and Imani, J., (2009), Plant cell and tissue culture: A tool in

biotechnology,Springer-Verlag Heidelberg Berlin.

Additional references supporting the course

Recommended

-

Prepared by:

Dr. ZarinaZainuddin

Kulliyyah of Science

Checked by:

Prof. Dr.Mohamad Osman

Coordinator

Department of Plant Sciences


Approved by:

Prof.

Dr.KamaruzzamanYunus

Dean


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Programme outcomes for Department of Plant Sciences

PO1 Knowledge Able to acquire and apply principles of plant sciences.

Able to understand the plant sciences-related concepts in relation to daily life

PO2 Practical Skills Able to conduct experiments and basic research in the field of plant sciences through laboratory work, final year project and

industrial training.

Able to familiarize and handle a few laboratory equipments and analytical methods.

PO3 Scientific Methods,

Critical Thinking &

Problem Solving Skills

Able to analyze and interpret laboratory data in plant sciences.

Able to apply knowledge of the relevant principles and analytical techniques to identify, solve and analyse certain

plant sciences problems.

Able to identify and solve plant science-related problem through effective thinking skills

Able to relate knowledge learned in plant science to relevant situations in life.

PO4 Communication Skills Able to deliver ideas in written form and/or through presentations and participations in various projects and

activities;

Able to demonstrate good communication orally and/or in written form and organization skills

Able to interpret the laboratory data and communicate their research findings through clear and accurate scientific reports.

PO5 Social Skills,

Teamworking &

Responsibility

Able to participate in various activities and demonstrate responsibility for the given tasks.

Able to work as an individual as well as in a team to complete the tasks given.

Able to understand the basic theory of leadership.

Able to demonstrate leadership skills in completing the tasks given

PO6 Life Long Learning &

Information

Management

Able to use knowledge gained for self development and continuous improvement.

Able to find/seek and manage plant sciences related information from many resources

PO7 Professionalism,

Humanities, Values,

Attitudes, Ethics

Able to understand and demonstrate the ethical and professional responsibilities

Able to demonstrate good work ethics, imbued with islamic moral-spiritual values and integrate the islamic knowledge and

values in the discipline to improve the quality of human life

and Ummah as a whole.

PO8 Managerial &

Entrepreneurial Skills Able to understand the basic knowledge or principles of

managerial and entrepreneurship related to plant sciences.

Able to identify and incorporate the knowledge learned into entrepreneurship opportunity in relation to plant sciences.

PO9 Leadership Skills Able to shows leadership qualities

PO10 Integration of Islamic

Revealed Knowledge

& Values

Able to integrate the knowledge of Plants Science with Islamic perspective for the benefit if the Ummah

sap2143- tissueculture course outline

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