sampling group 8
TRANSCRIPT
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Avenir,Chamique
Pacheco Gimuel
Alfred Serato
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A subset of a population on which the information is obtained
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Goal: Get an accurate picture of the population from the sample
RANDOM SAMPLING
Equal chance of being selected
Choose me!
Choose me!Choose me!
Choose me!
Choose me!
Choose me! Choose me!
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SIMPLE RANDOM SAMPLING
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Tools:1. Fishbowl/ Lots (with replacement)2. Random Number Table3. Random number generator in the
calculator or computer
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Random Assignment – every participant in the experiment has an equal chance of being assigned to any of the experimental or control conditions being compared.
Random Selection – every member of population has an equal chance of being selected to be a member of the sample.
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Systematic sampling
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Systematic sampling
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Convenience sampling
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Purposive Sampling
The selection of a sample is based on
previous knowledge of a population specific purpose of the research
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Purposive Sampling
different from convenience sampling
researchers do not simply study whoever is available
but use their judgement to select a sample which they believe will provide the data they need
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Coning and Quartering
Quadrat sampling
Transect Lines
Water Sampling
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Coning and Quartering
For granular or powdered samples
Procedure
• Form a conical heap• Spread it out into a circular flat cake• Divide the cake radially into
quarters• Choose two opposite quarters;
combine• Discard the other two quarters• Repeat as many times as needed to
get desired amount
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Coning and Quartering • Form a conical heap
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Coning and Quartering •Spread it out into a circular flat cake
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Coning and Quartering • Divide the cake radially into quarters
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Coning and Quartering •Choose two opposite quarters; combine
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Coning and Quartering •Discard the other two quarters
•Repeat as many times as needed to get desired amount
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Quadrat sampling
-A small area of ground marked out as sampleof a larger area
Purpose: to make a detailed description and record numerical data
For sampling objects that do not move –vegetation or more or less sedentary animals
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Quadrat sampling
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Quadrat sampling
A frame of predetermined area –placed on the ground
Data: number of individuals per unit area
Decisions:- quadrat size (usually 1m x 1m)- quadrat shape (usually square)- where to put it- how many quadrats- what rules to set
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Quadrat sampling
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Quadrat sampling
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Quadrat sampling
Random- Throwing quadrat
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Quadrat sampling
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Quadrat sampling
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Quadrat sampling
Systematic- using grids
- divide the area into squares of equal sizes
- locate the quadrats by picking coordinates randomly
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Quadrat sampling
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Quadrat sampling
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Transect Lines
- A line (usually straight) laid out on a sampling site-Observations are made in a systematic wayalong the line
Decision:- where to mark out the transect- how long- how many- how far apart- intervals between obseervations
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Transect Lines
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Transect LinesLine Transect- individuals touching the tape
are recorded
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-two parallel lines are marked out,individuals between these lines are recorded.
-similar to the line transect
-abundance, presence or absence of species
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Transect : Ladder Transect
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Considerations1)Type of water body.
2Tests to be performed on the sample.
3)Volume of the sample
4)Container to use
5)Cleaning procedure for container.
6)Water collection technique
7)Transporting and storage
8)How soon to process the sample
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- Collect a sample by the hand-dip method if the stream is well mixed.
-Open a sterile bottle ;grasp the bottle near the base , with hand and arm on downstream side of the bottle.
-Without rinsing , plunge the bottle opening downward below the water surface. Allow the bottle to fill with the opening point slightly upward into the current.
-Remove the bottle with the opening pointed upward toward the water and tightly cap it, allowing about 2.5 to 5 cm of headspace.
-This procedure minimizes the collection of the surface film and avoids contact with the streambed.
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1)Clean bottles and cap with dilate non phosphate, laboratory-grade
detergent, rinse it 3 times with hot tap and 3 times with deionized or distilled water and sterilize it by
autoclaving.
2)Collect approximately 800mL of stream water.
3)Leave at least an inch of headspace in the bottle to allow adequate mixing and aeration.
4)For E.Coli: Process the samples within 6
hours.
Store sample on ice in cooler or
refrigerator before processing.
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Aczel, Amir D. 1995, Statistics and Concepts and Applications, Chicago , Richard D . Irvin Inc.
Beach,David P. and Alvager, Torsten K.E,1992. Handbook for Scientific and Technical Research, New Jersey, Prentice Hall.
Brace, Charles H. and Brase, Corrine P. 1995. Understandable Statistics. Fifth Edition . Lexington, Massachussetts D.C Health and Company.
David, Fely P.,2002. Understanding and doing Research: A handbook for Beginners.TrialEdition. Iloilo City.
Kumar, Ranjit, 1996, Research Methodology. Australia Addison Wesley Longman. Rivera, Maximiano M. Jr. and Rivera, Roela Victoria,1996. Practical Guide to Thesis and Dissertation Writing. Manila, Philippines.
Sevilla Consuelo,G.et.al 1998. Research Methods, Revised Edition, Manila Rex bookstore.Field Sampling. Us geological Survey Ohio District Microbiology Laboratory. 2003. Online. Internet: http://oh.water.usgs.gov/micro/fldsmpl.html/(20 Oct 2006)
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Why it is advantageous to study a sample.
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Generating random numbers.
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Random Selection is employed when every member of a population has an equal chance of being selected to be a member of the sample.
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Cluster Sampling is a non-random sampling technique.
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Used for granular or powdery samples.
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To mark out a small area as a sample out of a large area.
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Two parallel lines are marked out and individuals between these lines are recorded.
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In water sampling.
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1) Give one reason why is it advantageous to study a sample.
a) Cheaperb) Fasterc) More Accurated) More Comprehensive
Information
2)Give one way of generating random numbers.
a) Fishbowl/Lots (with replacement)
b) Random Number Tablec) Random number
generator in the calculator or computer.
3) True or False: Random Selection is employed when every member of a population has an equal chance of being selected to be a member of the sample.
True
4) True or False: Cluster Sampling is a non-random sampling technique.
False.
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5) A sampling technique used for granular or powdery samples.
Coning and Quartering
6) A technique used to mark out a small area as sample out of a large area.
Quadrat
7)A sampling technique where two parallel lines are marked out and individuals between these lines are recorded.
Belt Transect.
8-9) Give two considerations in water sampling.
a. Type of Water body.b. Tests to be performed on the
sample.c. Volume of sampled. Container to usee. Cleaning procedure for containerf. Water collection techniqueg. Transporting and storageh. How soon to process the
sample.
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10) It is a subset of a population on which information is obtained.
Sample.
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