sample preparation catalog - hplc columns … preparation catalog 2 dear customer we are pleased to...
TRANSCRIPT
SPE cartridges, on-line SPE columns, QuEChERS
and associated products
based on
Molecularly Imprinted Polymers (MIP) sorbents
Polymeric sorbents
Modified Silica sorbents
2015
SAMPLE PREPARATION
CATALOG
2
Dear Customer
We are pleased to present the 2015 AFFINISEP sample preparation Catalog containing innovative
solutions to help you to achieve your goals. This year, we launch several innovative products for new
applications including our AFFINIMIP®SPE Multimyco LCMSMS dedicated to analysis of
multimycotoxins (including DON) before LC-MS/MS and AFFINIMIP®SPE GLYPHOSATE – AMPA. We
invite you to regularly visit our application notebook at www.affinisep.com that can help you in your
challenges.
At AFFINISEP,
Our R&D team is fully committed to developing quality analytical and diagnostic products that help
chemists get accurate information as simply as possible;
Our manufacturing organization supported by highly skilled sales and support teams is at your
disposal to satisfy you.
Your satisfaction is our engine.
Kaynoush Naraghi
CEO
TECHNICAL SUPPORTAFFINISEP has fully integrated technologies platform with specialized teams in organic chemistry,polymer chemistry, analytical and bioanalytical chemistry who are at your disposal to help you inyour challenges.
At AFFINISEP, we are committed to providing the best technical support possible. Our TechnicalSupport Group is a team of highly qualified M.Sc. and PhD Chemists, who are at your disposal toresolve your problem and to answer to your queries. For technical inquiries, feel free to contact useither by email: [email protected]
We are also very thankful to customer’s feedback about our products, protocols and customerservices by email to: [email protected]
www.affinisep.com
SOLID PHASE EXTRACTION – PRODUCT SELECTION GUIDE 4SORBENTS 5SOLID PHASE EXTRACTION 6QUICK LOOK-UP GUIDE 7Examples of SPE APPLICATIONS 8Formats available for SPE applications 11
AFFINIMIP®SPE (MOLECULARLY IMPRINTED POLYMER) 13AFFINIMIP® SPE – CONCEPTS 14AFFINIMIP® SPE FOR ANALYTICAL PURPOSE 15
Multimyco LCMSMS for Multimycotoxins analyses NEW! 17Fumonisins and Zearalenone (simultaneously) 18Zearalenone 19
Ochratoxin A 20Patulin 21Deoxynivalenol 22Estrogens 23Bisphenol A 24Amphetamines 25Chloramphenicol 26Tetracyclines 27Tamoxifen 28Zeranol residues 29Picolinic herbicides, Picloram, Aminopyralid, Clopyralid 30Glyphosate – AMPA NEW! 31Catecholamines 32
Metanephrines 33
Phenolics 34AFFINIMIP® SPE product list 35
AttractSPETM POLYMERIC CARTRIDGES 37
SELECTION OF THE AttractSPETM CARTRIDGES 38AttractSPE™ W/O -HLB - Hydrophilic Lipophilic Balance 39 AttractSPE™ DVB - Reversed Phase 39AttractSPE™ WAX - Weak Anion Exchange 39AttractSPE™ WCX - Weak Cation Exchange 40AttractSPE™ SAX - Strong Anion Exchange 40AttractSPE™ SCX - Strong Cation Exchange 40
Polymeric AttractSPETM CARTRIDGES – Product List 41
SilactSPETM SILICA BASED SORBENT SPE CARTRIDGES 43SilactSPETM SILICA-BASED SPE CARTRIDGES – Product List 46
On-Line SPE 49QcleanupTM - QuEChERS 51MYCOTOXINS STANDARDS 55SPE ACCESSORIES 57
CUSTOM-MADE PRODUCTS & SERVICES 59PROJECT DEVELOPMENT 61
ABOUT AFFINISEP 64ORDERING INFORMATION 64
TABLE OF CONTENTS
3
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SOLID PHASE EXTRACTION PRODUCT SELECTION
GUIDE
4
Sample preparation and Clean-up are the key steps in trace analysis for analytical chemist.
As specialist in this field, AFFINISEP supplies a complete range of solutions for sample
preparation based on Solid Phase Extraction process.
Solid phase extraction (SPE) is a powerful technique to provide a rapid and selective sample
clean-up, a high recovery and the concentration necessary for accurate quantitative analysis.
A multitude of phase chemistries and formats are available in this catalog for various
applications. We offer a comprehensive range of SPE sorbents to give you all elements to
face the increasingly complex and diverse sample preparation challenges to:
Simplify data analysis by removing interferences
Increase sensitivity and reliability by enrichment of the analyte
Obtain high and reproducible recovery yields from complex samples
In order to help you to select the appropriate product, you can check our Application
notebook which gathers a broad variety of analysis.
With this large number of phase chemistries available, different formats can be used:
•Open and reversible Solid Phase Extraction (SPE) cartridges or 96-wells plate for
automated or manual SPE
•On-line SPE columns for a direct coupling on a LC analysis. This format is very useful for
small sample extract or very sensitive analysis.
•QuEChERS with dispersive SPE or SPE cartridges for the clean-up process. This method was
mainly developed for the analysis of a high number of pesticides.
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SORBENTS
5
SPE Sorbents
AFFINISEP offers a complete range of sorbents for solid phase extraction from very specific
to a crude clean-up phases, from silica to polymers, from conventional to more sophisticated
sorbents for various applications.
AFFINIMIP® SPE
AFFINIMIP® SPE is a selective solid phase extraction based on Molecularly Imprinted
Polymers (MIP) that combines the advantages of immune-affinity columns regarding the
selectivity and those of polymeric SPE sorbents in terms of costs and robustness. Developed
for very problematic analyte purification, an instruction sheet gives all required information
to successfully carry out the analysis, including the protocol. No method development is
required. The protocol is easy, simple and fast. The chromatogram analysis is fast thanks to
the affinity between the analyte and AFFINIMIP® SPE. Methods are developed for the usual
analyte containing matrices. These matrices can be as diverse as aqueous matrices (food,
feed and biological matrices), fully organic matrices (oil), powder (milk) or cereals grains. If
your sample preparation is complex or gives inappropriate results, the development of an
AFFINIMIP® SPE can be a solution.
AttractSPETM Polymeric sorbents
AttractSPETM are the last generation of polymeric SPE sorbent. They are crosslinked polymer
particles bearing various chemical functionalities. A limited number of sorbent (6 products)
simplifies your choice and covers the full range of analysis using aqueous matrices.
These sorbents provide all the advantages of polymeric sorbents, including robustness (to
solvent, pH and T), simplified method development, wide applicability and not affected by
drying out. The combination of the water-wettable optimised surface chemistry, high
surface area and pH stability ensures high reproducible recoveries for a wide range of
analytes. For the same application, less sorbent and less solvent are required than for silica-
based sorbent and so, their added value is particularly obvious for trace analysis and for
small volume sample.
SilactSPETM Silica-based sorbents
The first sorbents historically used for SPE applications, Silica-based sorbents offer a broad
range of chemically modified sorbents. This chemistry goes from very polar sorbent (bare
silica) to hydrophobic sorbent (end-capped saturated hydrocarbon modified silica) passing
through intermediate polarity (for instance, amino modified silica). These sorbents are
sensitive to extreme pH. They are adequate for non-polar analytes in simple matrices.
QcleanupTM QuEChERS
QcleanupTM dispersive SPE products are a mixture of Magnesium Sulfate, PSA, Black carbon
or C18 used by QuEChERS method for the clean-up of fruits and vegetables during
multiresidues pesticides analyses.
www.affinisep.com 6
SPE PROCEDURE STEPS
Loading Washing Elution
Analytes of interest
Solid phase extraction requires the following steps:
1- Sample preparation: This step is necessary to obtain a loading solution compatible with
retention condition in the SPE column. In case of solid matrices, this solution must also
extract analytes from these matrices.
2- Conditioning: The SPE cartridges are conditioned with the appropriated solvents to fully
wet sorbent and enable further interactions between the analytes and the sorbent.
3- Loading: the percolation solution is loaded through the SPE cartridge. The analyte must
be retained in the column as well as unwanted compounds.
4- Washing: interferents and unwanted compounds are washed off by using several
solvents.
5- Elution: The desired analyte is extracted from the SPE cartridge.
Retention Mechanism
SPE cartridges are available in a complete and wide range of sorbents. A sorbent is often
classified according to the main retention mechanism between the analyte and the sorbent
(normal phase, reversed phase, ion-exchange, mixed-mode).
Normal phase retention mechanisms are based on polar-polar or dipole-dipole interactions
between the analyte and a silica-based sorbent.
Reversed phase retention mechanisms are based on non polar- non polar interactions and
Van der Waals dispersive forces. The sorbent is hydrophobic (polymeric sorbent or alkyl or
phenyl modified silica-based sorbent).
Ion-exchange retention mechanism use electrostatic interaction between a charged sorbent
and the ionic analyte. The sorbent is charged with the opposite charge of the analyte. A
cationic (respectively anionic) analyte interacts with a cation (respectively anion) exchange
sorbent.
Mixed-mode sorbents mainly interact through reversed phase and ion exchange retention
mechanisms. They are available as a polymeric sorbent (AttractSPETM SAX, WAX, WCX or
SCX) or as SilactSPETM.
SOLID PHASE EXTRACTION
www.affinisep.com 7
Analyte of interest
Existing AFFINIMIP® SPE
Selection of a sorbent
YESProblem solved
Ready to use Protocole
NO
AqueousNature of the
matrixWater miscible organic solvent
Not water miscible organic solvent
Nature of the analyte
ionic NeutralPolar to moderately
polarModerately to
Non Polar
Ion-Exchange AttractSPETM AttractSPETM DVB
Normal phase SilactSPETMAttract SPETM W/O - HLB
Sorbent
The choice of the sorbent is crucial for a successful sample pre-treatmentWhen AFFINIMIP®
SPE exists for the analyte, the choice is very simple and the problem is solved. The protocol
is simple and ready to use. AFFINIMIP® SPE have been developped for various trace analyses
in complex matrices and for problematic analysis with common SPE sorbents.
When no AFFINIMIP® SPE product exists, you can check the application notebook available
on our website to use the appropriate sample pretreatment.
In other cases, the sorbent chemistry must be chosen very carefully and each step of the
protocol must be optimized. This choice is based on the nature of the analyte and of the
matrix, on the loading condition and on the planned retention mechanism with the sorbent.
The following scheme gives a brief guideline to select the family of sorbents.
For specific applications such as trace analysis or complex matrices, conventional SPE
sorbents may give very complex analysis or unsatisfaying results. The development of an
AFFINIMIP® SPE sorbent for this application is a solution. We provide as well services for
method development.
QUICK LOOK UP GUIDE
For multiresidues analyses like pesticides, QcleanupTM QuEChERS and AttractSPETM W/O – HLB can beused.
Check the application notebook
www.affinisep.com
Analyte
Family SPE product- ANALYTES SPE product- ANALYTES MATRICES REF PAGE
Multimycotoxins
Aflatoxins, Ochratoxin A, HT-2, T-2,
Fumonisins, Zearalenone,
Deoxynivalenol
AFFINIMIP® SPE
Multimyco LCMSMSCereals FS118 17
Fumonisins AND ZearalenoneAFFINIMIP® SPE
FumoZON
Maize, Maize-based baby
foodFS109 18
Single Mycotoxin
ZearalenoneAFFINIMIP® SPE
Zearalenone
Maize, Wheat, Cereal-
based baby food, Rice,
Edible corn oil
FS100 19
Ochratoxin AAFFINIMIP® SPE
Ochratoxin A
Wheat, Maize, Pepper,
Paprika, Red and White
Wine
FS101 20
Patulin AFFINIMIP® SPE Patulin
All Apple-based products
(Juice, puree,
concentrate…)
FS102 21
Deoxynivalenol (DON)AFFINIMIP® SPE
DeoxynivalenolWheat, Maize, Oat FS117 22
Estrone, 17α-Estradiol, 17β-Estradiol,
Estriol, 17α- Ethynilestradiol
AFFINIMIP® SPE
Estrogens
Water, Serum, Plasma FS10423
Bisphenol A, Bisphenol AP, Bisphenol
AF, Bisphenol B, Bisphenol S, Bisphenol
F…
AFFINIMIP® SPE
Bisphenol A
A broad variety of liquid
and solid foodsFS106 24
ParabensAFFINIMIP® SPE
PhenolicsShampoo, cream FS103 34
Phenolic compoundsAFFINIMIP® SPE
PhenolicsFood matrices FS103 34
Amphetamine, Methamphetamine,
MDA, MDMA, MDEA
AFFINIMIP® SPE
AmphetaminesSerum, Urine DG102 25
Zeranol, Zearalanone, α and β
Zearalanol, α and β Zearalenol,
Resorcylic acid lactones
AFFINIMIP® SPE
Zeranol ResiduesUrine, Plasma FS105 29
ChloramphenicolAFFINIMIP® SPE
ChloramphenicolHoney, Urine, Shrimp FS110 26
TamoxifenAFFINIMIP® SPE
TamoxifenUrine PH101 28
Examples of SPE APPLICATIONS
8
Myc
oto
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sEn
do
crin
e
dis
rup
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Dru
gs r
esi
du
es
See our application notebook for more applications and details…
www.affinisep.com
ANALYTE
FAMILY SPE product- ANALYTES SPE product- ANALYTES MATRICES REF PAGE
Nicotine, Procaïnamide AttractSPETM W/O - HLB Urine HLB 39
Caffeine AttractSPETM W/O – HLB Urine, Water HLB 39
Propranolol AttractSPETM W/O - HLB Urine, Water HLB 39
Tetracyclines - Tetracycline, Oxytetracycline,
Chlortetacycline, Doxycycline
AFFINIMIP® SPE
TetracyclinesMilk FS112 27
Sulfonamides – Sulfadimethoxine ,Sulfaethoxypyridazine…
AttractSPETM SCX Milk SCX 40
Caffeine, Acetaminophen, Diclofenac, Ibuprofen,Ketoprofen, Naproxen, Carbamazepine
AttractSPETM W/O - HLBWaste water,
waterHLB 39
Antibacterial Aminoglycosides - - Streptomycin,Dihydrostreptomycin,…
AttractSPETM W/O - HLB Tissue, Milk HLB 39
Antibiotics – Quinolones, Macrolides,Lincosamides, Sulfonamides, Penicillins,Cephalosporine, Pleuromutilins, Diaminopyrimidine derivatives
AttractSPETM W/O - HLB Tissue, Milk HLB 39
NSAID (Non Steroidal Anti inflammatory drug)- Salicylic acid, Phenylbutazone, Flunixin,Tolfenamic acid, Meloxicam, Desoximethasome(IS), Ketoprofen
AttractSPETM W/O - HLB Tissue HLB 39
Penicillin based antibacterials - Ampicillin,Amoxicillin…
AttractSPETM W/O - HLB Tissue HLB 39
Glucocorticoids - Cortisone, Corticosterone,Aldosterone, Betamethasone, Dexamethasone,Flumethasone, Prednisone, Prednisolone,Methylprednisolone
AttractSPETM W/O - HLB Tissue HLB 39
Erythromycin and Clindamycin AttractSPETM W/O - HLB Tissue HLB 39
Praziquantel and Tiamulin AttractSPETM W/O - HLB Tissue HLB 39
Cephalexin AttractSPETM W/O - HLB Fish HLB 39
Quinoxaline-2 -carboxylic acid and 3-methyl
quinoxaline-2-carboxylic acidAttractSPETM SAX
Muscle, Liver,
Kidneys SAX 40
Vancomycin AttractSPETM SCX Fish SCX 40
Valnemulin and Tiamulin AttractSPETM W/O - HLB Fish HLB 39
Phenolic compounds AFFINIMIP® SPE Phenolicsbiological
matricesFS103 34
Examples of SPE APPLICATIONS
9
An
tib
ioti
cs a
nd
Dru
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esi
du
es
See our application notebook for more applications and details…
www.affinisep.com
ANALYTE
FAMILY SPE product- ANALYTES SPE product- ANALYTES MATRICES REF PAGE
Aminopyralid, Clopyralid, PicloramAFFINIMIP® SPE Picolinic
Herbicides
Water,
Compost, SoilFS115 30
Glyphosate, AMPAAFFINIMIP® SPE Glyphosate – AMPA
Waters FS113 31
16 common pesticides - Linuron, Iprodione,Desysopropylatrazine, Desethylatrazine,Aldocarb, Simazine, Carbofuran, Metalaxyl,Atrazin, 2, 4-D, Metazachlor, Dicloran,Phenmedipham, Procymidone, Fenitrothion,Vinclozolin
AttractSPETM W/O - HLB Water HLB 39
Triazine Herbicides - Simazine, Cyanazine,Atrazine…
AttractSPETM W/O - HLB Water HLB 39
Acetamide Herbicides - Metolachlor andmetabolites, Alachlor…
AttractSPETM W/O - HLB Water HLB 39
Fungicides - Carbendazim, Thiabendazole AttractSPETM SCX Fruit Juice SCX 40
Dopamine, Noradrenaline, Adrenaline, ...AFFINIMIP® SPE
Catecholamines
Plasma,
SerumDG100 32
Metanephrine, Normetanephrine and
3-Methoxytyramine, ...
AFFINIMIP® SPE
Metanephrines
Plasma,
SerumDG101 33
Hydroxylated Polycyclic Aromatic
Hydrocarbons - 2-Naphtol, 2-Hydroxyfluorene,
9-Phenanthrol…
AFFINIMIP® SPE PhenolicsContaminated
soilsFS103 34
Polycyclic Aromatic Hydrocarbons (PAH) AttractSPETM W/O - HLB Waste water HLB 39
Guaïacol AFFINIMIP® SPE Phenolics Wines, Water FS103 34
Carnosic acid AFFINIMIP® SPE Phenolics Meat, Water FS103 34
Hydroquinone AFFINIMIP® SPE Phenolics Water FS103 34
Melamine AttractSPETM SCX Milk, food SCX 40
Cyanuric acid AttractSPETM SAX Milk SAX 40
ARTIFICIAL SWEETENERS - Acesulfame,
Aspartame, Cyclamate, Neohespiridine
dihydrochalcone, Saccharin, Sucralose
AttractSPETM W/O - HLB Water HLB 39
COCAINE AND MAIN METABOLITES - Cocaine,
benzoylecgonine and ecgonine methyl ester
AttractSPETM W/O - HLBWaste water HLB 39
Examples of SPE APPLICATIONS
10
Oth
er
resi
du
es
and
Mis
cella
ne
ou
sP
est
icid
es
See our application notebook for more applications and details…
www.affinisep.com
Formats available on the catalog for SPE applications
Open Cartridge Reversible Cartridge
LRC Cartridge Cartridge for automate
On-line SPE cartridge 96 Well-plates
Format: 0,7mL ; 2 mLMaterial: PolypropyleneFrits: Polyethylene
Luer compatible
Format: 1 mL; 3 mL; 6 mL; 15 mL; 20mL; 60mL… Material: Polypropylene ; glass (6mL)Frits: Polyethylene ; PTFE (glass cartridges); Glass fiber (Glass cartridges)
Luer compatible
Luer compatible
Format: 10mLMaterial: PolypropyleneFrits: Polyethylene
Cartridge for Multipurpose Sampler (GERSTEL)Format: 1 mL; 3 mL; 6 mLMaterial: PolypropyleneFrits: Polyethylene
Luer compatible
Cartridge for ASPEC (GILSON)Compatible with Open cartridges
FORMATS AVAILABLE FOR SPE APPLICATIONS
For other formats, please contact us
I.D. 2,1 and 4,6mmLength: 20mm
11
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Food / Feed Safety
Environment
Cosmetics
Pharmaceutical R&D
AFFINIMIP® SPESelective Solid Phase ExtractionMolecularly Imprinted Polymers for the Selective
Extraction of Trace Analytes from Complex Matrices
13
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New Extraction Phase Based on Molecularly Imprinted Polymers
Molecularly imprinted polymer is a polymer with a «memory» of the shape and functional
groups of a template molecule. AFFINISEP designs this material in order to recognize
selectively the target molecule, even in the presence of compounds with structure and
functionality similar to those of the template. Using an imprinting process, we design a
material with high affinity for a target molecule:
AFFINIMIP® SPE - CONCEPTS
Advantages of SPE based molecularly imprinted polymers
Molecular Recognition materialsAFFINISEP designs these materialsfor an broad variety of molecules,such as natural products(mycotoxines, sugars…) ,pharmaceuticals, peptides etc…
High affinity and selectivity
Ensure analysis Reproducibilityand reliability via robustand rapid methodology
AFFINIMIPSynthetic materials
Analytical and preparativeapplications in research andproduction
Stable to•pH variation•Temperature variation•Organic solvents
Selective Solid Phase Extraction
Molecularly Imprinted Polymers for theSelective Extraction of Trace Analytes fromComplex Matrices
14
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AFFINIMIP® SPE is a selective solid phase
extraction based on Molecularly Imprinted
Polymers (MIP) that combines the
advantages of immune-affinity columns
regarding the selectivity and those of a
classic Solid Phase Extraction (SPE) in terms
of costs and robustness.
AFFINIMIP® SPE Selective Extraction
Cartridges
Perfect clean-up system for trace analysis
Thanks to selectivity of AFFINIMIP®SPE,
stringent washing steps can be applied in
order to remove all interferences and thus
minimize matrix effects. It also reduces
ion-suppression effects.
Minimal to no method development
required
A protocol based on three steps (loading,
washing and elution) is supplied with
AFFINIMIP®SPE kits for tested matrices.
No extra-equipment than the usual
required for SPE experiments is necessary.
AFFINIMIP® SPE FOR ANALYTICAL PURPOSE
AFFINIMIP® SPE protocols are as well
defined by 3 steps of loading, washing and
elution. All steps have been already
developed in detail by AFFINISEP and an
instruction sheet is supplied with the
product.
The advantages of AFFINIMIP® SPE are
essential in trace analysis from a complex
matrice in food safety, environment
and pharmaceutical analysis such as
Mycotoxins (Patulin, Ochratoxin A,
Zearalenone), Phenolics, Endocrines
Disruptors (Estrogens, Bisphenol A), Drug
residues (Amphetamines, Zeranol residues)
The SPE protocol is supplied in an
instruction sheet for various complex
matrices.
For other matrices, please contact our
technical support to help you with your
application.
15
Example of an instruction sheet supplied with AFFINIMIP® SPE
www.affinisep.com
Mycotoxins analyses
Mycotoxins are toxic secondary metabolites produced by different fungi present in
agricultural commodities. They are regulated in food and feed due to nephrotoxic,
neurotoxic, carcinogenic, estrogenics, and immunosuppressive effects.
AFFINISEP has developed two sets of product for mycotoxins analyses:
For single mycotoxin extraction
Designed for the analysis of one specific mycotoxin or for mycotoxins analyzed by
fluorescence detection
AFFINISEP has developed the following products
AFFINIMIP® SPE Patulin
AFFINIMIP® SPE Ochratoxin A
AFFINIMIP® SPE Zearalenone
AFFINIMIP® SPE Fumonisins
AFFINIMIP® SPE Deoxynivalenol
For multimycotoxins extraction
Designed for the simultaneous extraction of several mycotoxins which are present in a
same matrix prior to LC-MS/MS analyses
These mycotoxins are all present in the same matrix to be analyzed. Their extraction is done
all at once by SPE. Then the elution solution containing all these mycotoxins is evaporated,
reconstituted and analyzed by LC-MS/MS.
The protocol is short and efficient
AFFINISEP has developed the following products
AFFINIMIP® SPE FumoZON for the analyses of
Fumonisins
Zearalenone
AFFINIMIP® SPE Multimyco LCMSMS for the analyses of
Fumonisins
Aflatoxins
Ochratoxin A
T-2 and HT-2
Zearalenone
Deoxynivalenol
16
www.affinisep.com
AFFINIMIP® SPE MultimycoLCMSMS
• SIMULTANEOUS Extraction
• All at once extraction of
- Fumonisins,
- Aflatoxins,
- Ochratoxin A,
- T-2 and HT-2
- Zearalenone (ZON)
- Deoxynivalenol (DON)
• Developed for analysis by LC-MS/MS
• Ready for use and optimized extraction methodology adapted to
cereal matrices
• Fast and very simple protocol
AFFINIMIP® SPE Multimyco LCMSMS are Multimycotoxins solid-phase extraction cartridges that
selectively and SIMULTANEOUSLY clean-up and concentrate Fumonisins, Aflatoxins, Ochratoxin A, T-
2, HT-2, Zearalenone and Deoxynivalenol prior to analysis by LC-MS/MS from complex matrices such
as cereals. In Europe, these mycotoxins are all regulated and they are also present in the same
matrices.
FS118
Ordering information: Catalog number: FS118-02 for 25 cartridges, 3mL
FS118-03 for 50 cartridges, 3mLFS118-04 for 100 cartridges, 3mLFS118-02B for 25 cartridges, 6mLFS118-03B for 50 cartridges, 6mL
Recovery of multimycotoxins analyzed after
AFFINIMIP® SPE Multimyco LCMSMS cleanup
Product Information
Tested matrices : Cereals
Protocol :
Loading : Acetonitrile - Water
Washing : Acetonitrile - Water - AA
Elution : Methanol – Acetonitrile - Formic
acid
Detection : HPLC-MS/MS
Format : 3mL cartridges
Particle diameter range : 25-80 µm
Shipping & Storage : Ambient temperature
For other matrices or formats, please contact
us for technical support
Recovery yield
17
Compound name C° µg/kg R%
Aflatoxin B1 0,4 80
Fumonisin B1 4 75
HT-2 4 97
T-2 4 96
Zearalenone 10 95
Ochratoxin A 4 83
Deoxynivalenol 10600 82
CEREALS
www.affinisep.com
AFFINIMIP® SPE FumoZON
•SIMULTANEOUS Selective Extraction of Fumonisins B1+B2 and
Zearalenone (ZON) for analysis by LCMS
•Ready for use and optimized extraction methodology adapted to
maize matrices
•Strong reduction of Ion suppression
•Very simple protocol and high capacity cartridges
AFFINIMIP® SPE FumoZON are selective solid-phase extraction cartridges that selectively and
SIMULTANEOUSLY clean and concentrate Fumonisins and Zearalenone prior to analysis by HPLC from
complex matrices such as maize and cereal-based baby food. In Europe, Regulation (EC)
N°1126/2007 sets maximum levels for Zearalenone and Fumonisins in foodstuffs with respectively
20µg/kg and 200µg/kg for maize-based babyfood.
FS109
Ordering information: Catalog number: FS109-02 for 25 cartridges, 3mL
FS109-03 for 50 cartridges, 3mL
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Zearalenone
Fumonisin B1
Fumonisin B2
Chromatograms obtained after AFFINIMIP® SPEFumoZON Clean-up of a maize flour spiked at38µg/kg with Zearalenone, 2408µg/kg withFumonisin B1 and 630µg/kg with Fumonisin B2.
SampleC°
µg/kgMeanµg/kg
Recoveries %
% RSDR
Zearalenone 20 16.9 84.4 1.6 (n=4)
Fumonisin B1 200 168.6 84.3 1.4 (n=3)
Fumonisin B2 200 185.6 92.81.9
(n=3)
Recovery of Zearalenone, Fumonisins B1 and B2 in
maize-based baby food after AFFINIMIP® SPE
FumoZON cleanup
Product Information
Tested matrices : maize matrices
Capacity: Higher than 10µg/g Zearalenone and
5µg/g Fumonisins B1 and B2
Protocol :
Loading : Methanol – Acetonitrile - Water
Washing : Acetonitrile - Water
Elution : Methanol - Acetic acid
Detection : HPLC-MS ESI
Format : 3mL cartridges
Particle diameter range : 25-80 µm
Shipping & Storage : Ambient temperature
For other matrices or formats, please contact
us for technical support
Publications
Application of molecularly imprinted polymers
to determine B1, B2, and B3 fumonisins in
cereal products, M. Bryła, R. Jędrzejczak, M.
Roszko, K. Szymczyk, M. W. Obiedziński, J.
Sękul, M. Rzepkowska, J. Sep. Sci., 36(3), 578-
584, 2013.
Application note - AFFINIMIP® SPE FumoZON -
Maize and Maize-based baby food
MAIZE
Recovery yield
18
www.affinisep.com
0
20000
40000
60000
80000
100000
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140000
0 1000 2000 3000 4000 5000 6000 7000
AFFINIMIP®SPE Zearalenone are selective solid-phase extraction cartridges for Zearalenone from
complex matrices such as Maize, Cereal-based Baby Food and Rice.
In Europe, Regulation (EC) N°1881/2006 sets maximum levels for Zearalenone in foodstuffs such as
100µg/kg in cereals and 20µg/kg in maize-based babyfood.
AFFINIMIP® SPE Zearalenone
Ordering information: Catalog number: FS100-02 for 25 cartridges, 3mL
FS100-03 for 50 cartridges, 3mL
FS100
Perfect clean-up method for trace
analysis of raw and cooked food
matrices
Fluorescence chromatograms obtained after a
purification with AFFINIMIP® SPE Zearalenone of a cereal
sample (80µg/kg of Zearalenone)
Time (min)
mV
With AFFINIMIP® SPE Zearalenone
No pre-treatment
CEREAL SAMPLE
Zearalenone
Product Information
Tested matrices : Wheat, Maize, Cereal-based
baby food, Edible corn oil and Rice
Capacity: Higher than 10µg/g Zearalenone
Protocol :
Loading : Acetonitrile - Water
Washing : Acetonitrile - Water - Acetic Acid
Elution : Methanol - Acetic acid
Detection : HPLC-Fluorescence
(excitation/emission wavelengths of 275 /
450nm)
Format : 3mL cartridges
Particle diameter range : 25-80 µm
Shipping & Storage : Ambient temperature
For other matrices or formats, please contact
us for technical support
Publications
Molecularly imprinted polymer solid-phase
extraction for detection of zearalenone in
cereal sample extracts,
Analytica Chimica Acta, 672, 15–19, 2010.
Application note - AFFINIMIP® SPE Zearalenone
- Cereal Products and Baby Food
Application note - AFFINIMIP® SPE Zearalenone
- Automated Protocol (GILSON GX271
ASPEC™)
Application note - AFFINIMIP® SPE Zearalenone
– Edible corn oil
High capacity of AFFINIMIP®SPE in
comparison with Immuno Affinity Columns
Comparative study of the capacity between
Immuno-Affinity Columns and AFFINIMIP®SPE
Zearalenone
Zearalenone (ng)
AFFINIMIP®SPE Zearalenone
Immuno-Affinity Columns
Are
a
High capacity - No need of high
dilution
Very simple protocol for complex
matrices
19
www.affinisep.com
AFFINIMIP®SPE Ochratoxin A are selective solid-phase extraction cartridges for Ochratoxin A that
selectively clean and concentrate the toxin prior to analysis by HPLC from matrices such as Wheat,
Maize, Pepper, Paprika, Red and White Wine.
In Europe, Regulation (EC) N°1881/2006 sets maximum levels for Ochratoxin A in foodstuffs such as
5µg/kg in cereals and 2ppb in wine. The regulation (EC) N°105/2010 also defines maximum levels for
spices and liquorice products.
AFFINIMIP® SPE Ochratoxin A
Recovery yield
MatrixC°
Ochratoxin A (µg/kg)
Recoveries %
% RSD
Wheat (n=6) 5 96.3 7.7
Paprika (n=4) 30 93.3 3.4
Grey Pepper (n=3)
30 73.3 5.7
White wine (n=10)
2 91.3 6.2
Red wine (n=4) 2 78.8 2.8
FS101
Time (min)
mV
Fluorescence chromatograms obtained after a
purification with AFFINIMIP® SPE Ochratoxin A of a
wheat sample (2.5µg/kg of Ochratoxin A)
Ochratoxin A
With AFFINIMIP®SPE Ochratoxin A
No pre-treatment
WHEAT
0 2 4 6 8 10 12 14 16 18 20 22 24 26 280
1
2
3
4
5
6
7
8
9
Product Information
Tested matrices : Wheat, Maize, Paprika, red and
white Wine, Pepper
Protocol :
Loading : loading solution
Washing : Acetonitrile - HCl solution
Elution : Methanol - Acetic acid
Detection : HPLC-Fluorescence excitation/emission
wavelengths of 333 and 460nm
Format : 3mL and 6mL cartridges
Particle diameter range : 25-80 µm
Shipping & Storage : Ambient temperature
For other matrices or formats, please contact us
for technical support
Publications
Solid-phase extraction using molecularly imprinted
polymers for selective extraction of a
mycotoxin in cereals, Journal of
Chromatography A, 1217, 6668-6673, 2010
Molecularly imprinted polymer-based solid phase
clean-up for analysis of ochratoxin A in ginger
and LC-MS/MS confirmation, Cao J., Zhou S.,
Kong W., Yang M., Wan L., Yang S., Food
control, 33(2), 337-343, 2013.
Application note - AFFINIMIP® SPE Ochratoxin A -
Cereals and Spices
Application note - AFFINIMIP® SPE Ochratoxin A -
Red and White Wine
Other publications and application note on our
website
•Ready for use and optimized extraction protocols adapted for
each tested matrices
•Perfect clean-up system for trace analysis of Ochratoxin A
•Unambiguous and reliable analysis
20
Ordering information:Catalog number: FS101-02 for 25 cartridges, 3mL
FS101-03 for 50 cartridges, 3mLFS101-02B for 25 cartridges, 6mLFS101-03B for 50 cartridges, 6mL
www.affinisep.com
AFFINIMIP® SPE Patulin
AFFINIMIP® SPE Patulin are selective solid-phase extraction cartridges
that selectively clean and concentrate this toxin prior to analysis by HPLC
from complex matrices such as apple juice, compote, apple puree
including based on baby food. The World Health Organization, the FDA
and the European Union recommend a maximum concentration of 50
µg/L in apple juice and 25µg/kg in apple puree.
MatrixC° Patulin (ng/mL)
Recoveries %
% RSD
Apple juice (n=4) 25 86 10
Apple juice (n=4) 46 86 10
Apple juice (n=4) 10 83 15
Apple puree(n=3)
20 84 4.5
Baby food applepuree (n=4)
10 81.2 2.1
Ordering information:Catalog number:For apple puree and apple juiceFS102-02 for 25 cartridges, 3mL-100mg sorbentFS102-03 for 50 cartridges, 3mL-100mg sorbentFor all apple products and fruit juice, concentratedfruit juiceFS102-02B-200mg for 25 cartridges, 6mL-200mgFS102-03B-200mg for 50 cartridges, 6mL-200mg
FOR KIT Cartridges + pectinase see on p 30
Recovery yield
FS102m
AU
Time (min)
Patulin
APPLE JUICE
•Unique extraction method available on the market
•Easier and faster than Liquid – Liquid Extraction
•Perfect clean-up system for trace analysis of Patulin
•Ready for use and optimized extraction protocol
•Applicable to several apple derived matrices
•Considerable decrease of 5-Hydroxymethylfurfural (HMF) signalProduct InformationTested matrices: Apple juice (clear & cloudy), Apple
and Multifruit puree, baby food, cider, Alcoholpommeau and manzella, Dried apple (format200mg)
Protocol:Loading: Diluted apple juice or apple pureeafter treatment with pectinase enzyme.Washing: NaHCO3 – Water - Diethyl EtherElution: Ethyl acetate
Detection: HPLC-UVFormat: 3 and 6mL cartridgesParticle diameter range : 25-80 µmShipping & Storage : Ambient temperatureFor other matrices or formats, please check ourapplication notebook PATULIN on our website orcontact us for technical support
PublicationsApplication note - AFFINIMIP® SPE Patulin - Apple Juice
and Apple PureeApplication note - AFFINIMIP® SPE Patulin - Baby FoodApplication note - AFFINIMIP® SPE Patulin - CiderApplication note - AFFINIMIP® SPE Patulin - Multifruit
PureeApplication note - AFFINIMIP® SPE Patulin – Dried appleApplication note - AFFINIMIP® SPE Patulin 6mL -150mg -
apple puree
7 8 9 10 11 12 13 14 15 16 17 18 19 20
-
7 8 9 10 11 12 13 14 15 16 17 18 19 20
-10
-5
0
5
10
15
20
25
30
35
40
45
50
55
LC-UV chromatograms obtained after AFFINIMIP®SPE
Patulin Clean-up of an apple juice spiked at 10µk/kg with
Patulin (blue) and not spiked (Red)
21
www.affinisep.com
AFFINIMIP® SPE Deoxynivalenol
•High recovery yield (>80%)
•Efficient clean-up on cereals
•Ready to use and robust protocols
Ordering information:Catalog number: FS117-02B for 25 cartridges, 6mL
FS117-03B for 50 cartridges, 6mL
FS117
AFFINIMIP® SPE Deoxynivalenol are selective solid-phase extraction cartridges for the extraction of
Deoxynivalenol (DON) on cereals prior to analysis by HPLC from cereals such as Oat, Wheat, Corn. In
Europe, Regulation (EC) N°1126/2007 sets maximum levels for Deoxynivalenol in cereals with, for
instance, respectively 1750µg/kg for unprocessed maize, 750µg/Kg for cereal flours and 200µg/kg for
babyfood.
Product information
Tested matrices : oat, wheat, corn
Protocol :
Loading: Water/Acetonitrile/Formic acid
Washing : Water – NaHCO3 - Diethylether
Elution : Ethyl acetate
Detection : LC-MS / UV
Format : 6mL cartridges
Particle diameter range : 25-80 µm
Shipping & Storage : Ambient temperature
For other matrices or formats, please contact
us for technical support
CORN
UV chromatograms of corn spiked with DON(800µg/Kg) (repeated twice) and not spiked (red)after AFFINIMIP®SPE Deoxynivalenol clean-up
RT: 0.00 - 7.96
0 1 2 3 4 5 6 7 Time (min)
500 1000 1500 2000 2500 3000 3500 4000 4500 5000 5500
Intensity
Deoxynivalenol
LC-MS chromatograms of corn spiked with DON(800µg/Kg) (repeated twice) and not spiked (red)after AFFINIMIP®SPE Deoxynivalenol clean-up
Deoxynivalenol
RT: 0.00 - 7.98
0 1 2 3 4 5 6 7 Time (min)
-30000 -20000 -10000
0 10000 20000 30000 40000 50000 60000 70000 80000 90000
100000 110000 120000
uAU
Recovery yields >80%
Recovery yields >80%
Recovery of corn spiked with DON (800µg/Kg) afterAFFINIMIP®SPE Deoxynivalenol clean-up
Recoveries %
% RSD
Reproductibility (UV, n=6) 83 2
Reproductibility (MS, n=6) 86 7
Repetability (UV, n=3) 83 2
Repetability (MS, n=3) 83 1
21
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AFFINIMIP® SPE Estrogens
•Extraction of a broad family of natural and synthetic estrogens by
direct percolation of diluted plasma or water
•Fast and simple protocol
MRM chromatograms from GC-MS/MS analysis of fortified calves’ plasma samples at 0, 10, 40 and 100 pg.mL-1
with 17α-estradiol, 17β-estradiol and estrone. Chromatograms obtained after a clean-up with AFFINIMIP® SPEEstrogens (Courtesy of Emmanuelle Bichon - LABERCA)
BOVINE PLASMA
Publications
Solid-phase extraction using molecularly imprinted
polymer for selective extraction of natural and
synthetic estrogens from aqueous samples, Paolo
Lucci et al., J.Chrom. A, 1218(30), 4828-4833, 2011.
Quantification of estrogens at ppt levels in bovine
plasma by Molecularly Imprinted Solid Phase
Extraction and GC-MS/MS analysis, Emmanuelle
Bichon et al. (LABERCA) Poster session,
Euroresidues VIIth conference (2012).
High efficiency of semi-preparative Supercritical
Fluid Chromatography with Molecularly Imprinted
Polymer as stationary phase (SFC-MIP). Application
on urinary steroids purification for IRMS analysis,
M. Doué et al., HTSP2 2012.
And other publications on our website
Ordering information:Catalog number: FS104-02A for 25 cartridges, 1mL
FS104-03A for 50 cartridges, 1mLFS104-02 for 25 cartridges, 3mLFS104-03 for 50 cartridges, 3mL
FS104
AFFINIMIP® SPE Estrogens are selective solid-phase extraction cartridges that selectively clean and
concentrate the natural or synthetic estrogens family prior to further analysis from complex matrices
such as Water, Plasma or Serum.
Product Information
Tested matrices : Water and plasma.
Protocol :
Loading : Water or diluted plasma
Washing : Acetonitrile - Water
Elution : Methanol
Detection : LC-MS
Format : 3mL cartridges
Particle diameter range : 25-80 µm
Shipping & Storage : Ambient temperature
For other matrices or formats, please contact
us for technical support
23
Blank plasma 10ppt 40ppt 100ppt
17β-Estradiol-d3
419>285
17 α/β-Estradiol416>129
17 α/β-Estradiol416>285
www.affinisep.com
AFFINIMIP® SPE Bisphenol A
Ordering information:Catalog number:FS106-02 for 25 cartridges, 3mL PP cartridgesFS106-03 for 50 cartridges, 3mL PP cartridgesFS106-02B for 25 cartridges, 6mL PP cartridgesFS106-03B for 50 cartridges, 6mL PP cartridgesFS106-02G for 25 cartridges, 6mL glass cartridgesFS106-03G for 50 cartridges, 6mL glass cartridges
FS106
AFFINIMIP® SPE Bisphenol A are selective solid-phase extraction cartridges that selectively clean and
concentrate Bisphenol A prior to analysis by HPLC from complex matrices such as River Water or
Babymilk. It is known as an endocrine disruptor and has been banned in plastic infant feeding bottles
in several countries (Canada, EU (directive 2011/8/EU)).
•Perfect clean-up system suitable for all chromatography
techniques: LC, GC, MS...
•Easy quantification of Bisphenol A with fluorescence detection :
no derivatization as needed in GC
•2 grades: glass and polypropylene cartridges
LIQUID INFANT FORMULA
Time (min)Minutes
0 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 175 6 7 8 9 10 11 12 13 14 15 16 170 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19
0
100
200
300
400
500
600
700
800
900
1000
1100
Minutes
0 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17Minutes
0 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 175 6 7 8 9 10 11 12 13 14 15 16 170 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19
0
100
200
300
400
500
600
700
800
900
1000
1100
Injection of 50µL of infant formula before clean-up
Bisphenol A after clean-up of 15mL of infant formula
Fluorescence chromatograms of infantil formula
spiked with 1µg/L Bisphenol A before (Red) and after
purification (Blue) with AFFINIMIP® SPE Bisphenol A.
24
Product Information
Tested matrices : Water, milk (infant formula),
powdered infant formula or canned food,
vegetable puree for infant, Beer…
Protocol:
Loading: Matrix
Washing: Water - Acetonitrile
Elution: Methanol
Format:
Polypropylene cartridges 3mL and 6mL
Glass cartridges 3mL with Teflon frits: max.
quantity BPA < 5ng/cartridge
Particle diameter range : 25-80 µm
Shipping & Storage : Ambient temperature
For other matrices or formats, please check ourapplication notebook BISPHENOL A on our websiteor contact us for technical support
PublicationsApplication note - AFFINIMIP® SPE Bisphenol A - Beer
Application note - AFFINIMIP® SPE Bisphenol A - infant
formula
Application note - AFFINIMIP® SPE Bisphenol A -
Powdered infant formula
Application note - AFFINIMIP® SPE Bisphenol A – Canned
food
Application note - AFFINIMIP® SPE Bisphenol A –
Vegetable puree in plastic food packaging
www.affinisep.com
AFFINIMIP®SPE Amphetamines
•Extraction of Amphetamine and Methamphetamine derivatives by
direct percolation of diluted serum or urine
•High capacity cartridge for reliable results at high concentrations
Recoveries >85% for each analyte
•Reduction of sample preparation time
DG102
AFFINIMIP® SPE Amphetamines are selective solid phase extraction cartridges that selectively clean
and concentrate Amphetamine and Metamphetamine derivatives from complex matrices such as
Serum or Urine.
Amphetamine has a high possibility of severe psychological and physiological dependence.
As a stupefiant, Amphetamines consumption is prohibited in main european countries. French
regulation has set up an analytical threshold of 50ng/mL in blood for drivers.
Ordering information:Catalog number: DG102-02 for 25 cartridges, 3mL
DG102-03 for 50 cartridges, 3mL
Time (min)
Mass chromatogram (SIM) of 0.5mL of serum spikedwith 100ng/mL of each Amphetamine andMethamphetamine derivatives after clean-up withAFFINIMIP® SPE AmphetaminesAmphetamine (m/z=136); Methamphetamine(m/z=150); MDA: 3,4-Methylenedioxyamphetamine(m/z=180); MDMA, 3,4-Methylenedioxymethamphetamine (m/z=194);MDEA, 3,4-Methylenedioxy-N-ethylamphetamine(m/z=208).
Analyte Recoveries % % RSD
Amphetamine 91.0 5.1
Methamphetamine 90.7 1.9
MDA 92.0 3.7
MDMA 92.2 2.5
MDEA 98.2 5.0
Recovery yield
Recovery yield and %RSD for serum sample spikedwith 100 ng/mL (n=4)
2 4 6 8 10 12 14 16 18 20 22 240
10
20
30
40
50
60
70
80
90
Amphetamine
Methamphetamine
MDA
MDMA
MDEA
SERUM
Product Information
Tested matrices : Urine and serum
Protocol :
Conditioning : Acetonitrile and Deionized
Water Loading : Diluted urine or serum
Washing : W1: Deionized Water
W2: Acetonitrile -
Deionized Water
Elution : Methanol - Formic acid
Detection : HPLC-MS ESI positive mode
Format : 3mL cartridges
Particle diameter range : 25-80 µm
Shipping & Storage : Ambient temperature
For other matrices or formats, please contact
us for technical support
Publications
Application note - AFFINIMIP® SPE
Amphetamines - Human urine and serum
25
www.affinisep.com
AFFINIMIP®SPE Chloramphenicol
•Very simple and fast sample preparation
•Direct percolation of honey diluted in water
•High loading capacity: Up to 10g of honey analysed
•Perfect clean-up system for trace analysis of Chloramphenicol
•Detection of 0.3µg/kg with a single quatrupole Mass spectrometer
FS110
AFFINIMIP® SPE Chloramphenicol are selective solid phase extraction
cartridges that selectively clean and concentrate Chloramphenicol from
complex matrices such as Honey.
Chloramphenicol is a broad-spectrum antibiotic widely used in the world in
the past. Several health problems are related by its use. As a consequence,
several countries (e.g. U.S.A, E.U, Canada…) have prohibited its use for food-
producing animals. As no permited limit has been established, E. U has
defined a minimum required performance limits (MRPLs) of 0.3µg/kg for
products of animal origin (Commission decision 2003/181/EC).
However, food matrices are very complexes and induce ion-suppression
phenomena which distort analysis results. AFFINIMIP® SPE Chloramphenicol
solves this problem.
Ordering information:Catalog number: FS110-02A for 25 cartridges, 1mL
FS110-03A for 50 cartridges, 1mL
2 3 4 5 6 7 8 9 10
1000
2000
3000
4000
5000
6000
7000
8000
9000
10000
2 3 4 5 6 7 8 9 10
1000
2000
3000
4000
5000
6000
7000
8000
9000
10000
MS chromatogram (SIM) of several honeys spiked
with 15.7 µg/kg of Chloramphenicol after clean-up
with AFFINIMIP® SPE Chloramphenicol
Blue traces are not spiked samples
Recovery yield > 90%
Time (min)
inte
nsi
ty
Product Information
Tested matrices : Honey, Bovine Urine
Protocol:
Loading: Diluted honey
Washing: ACN – Water – NH3 – Diethyl
ether
Elution: Methanol
Format: Polypropylene cartridges 1mL
Particle diameter range : 25-80 µm
Shipping & Storage : Ambient temperature
For other matrices or formats, please contact
us for technical support
Publications
Application note - AFFINIMIP® SPE
Chloramphenicol – Honey
Application note - AFFINIMIP® SPE
Chloramphenicol – Bovine Urine
Application note - AFFINIMIP® SPEChloramphenicol – Shrimp
HONEY
26
www.affinisep.com
AFFINIMIP®SPE Tetracyclines
•Very simple and fast sample preparation
•Ready for use and optimized protocols
•Perfect clean-up system for trace analysis of Tetracycline,
Oxytetracycline and Chlortetracycline
•LLC-UV or LC-MS/MS
FS112
AFFINIMIP® SPE Tetracyclines are selective solid phase extraction cartridges that selectively clean
and concentrate Chloramphenicol from complex matrices such as Milk.
Tetracyclines (TCs) like oxytetracycline (OTC), tetracycline (TC) and chlortetracycline (CTC) are broad-
spectrum antibiotics and are widely used as veterinary medicines and feed additives. These residues
can cause toxic or allergic reactions in hypersensitive individuals and also transfer drug-resistant
bacteria from food to humans.
In response to these concerns and to prevent harmful effects of residual antibiotics in milk on the
human health, various international health organizations have established the maximum residual
limit (MRL) of TCs in all circulating milk in their countries. Worldwide maximum residue levels (MRL)
for tetracycline antibiotics are 100ppb (µg/L).
For such low concentrations, a clean up step is crucial in order to improve the sensitivity, the
reliability and the specificity before LC analysis.
Ordering information:Catalog number: FS112-02A for 25 cartridges, 1mL
FS112-03A for 50 cartridges, 1mL
Product Information
Tested matrices : Milk
Protocol:
Loading: Milk
Washing: ACN – Water
Elution: Methanol – Formic acid
Format: Polypropylene cartridges 1mL
Particle diameter range : 25-80 µm
Shipping & Storage : Ambient temperature
For other matrices or formats, please contact
us for technical support
Publications
Application note - AFFINIMIP® SPE Tetracyclines
– Milk
MILK
27
UV Chromatograms (355nm) obtained after clean-up with AFFINIMIP® SPE Tetracyclines of 1.5mL ofMilk spiked with Tetracyclines at 50µg/L (blue) ornot spiked (red) or of 1.5mL of water spiked withTetracyclines at 50µg/L (pink)
Minutes
0.0 5.0 10.0 15.0 20.0 25.0 30.0 35.0 40.0 45.0 50.0
mA
U
0
10
20
30
40
50
60
TCOTC
CTC
Recovery of Tetracyclines spiked at 50µg/L afterAFFINIMIP® SPE Tetracyclines clean-up of 1.5mL ofMilk and relative standard deviation calculatedfrom results generated under reproducibilityconditions.
TetracyclinesC°
(µg/L)Mean (µg/L)
Recoveries %
% RSDR
(n=3)
Tetracycline 50 44.3 88.7 9.5
Oxytetracycline
50 53.7 107.3 10.7
Chlortetracycline
50 40.7 81.3 9.6
www.affinisep.com 28
Since January 2000, Tamoxifen (antioestrogenic molecule) has been included
in the list of prohibited substances by the International Olympic Committee.
So, the presence of these compounds in urine is a doping proof. The analysis
of complex samples, like biofluids, requires a sample preparation step prior to
analysis. AFFINIMIP® SPE Tamoxifen is the perfect tool for selectively extract
Tamoxifen and its main metabolite in biofluids, such as urine (clean-up and
pre-concentration of sample at trace levels). The recoveries of Tamoxifen and
its metabolite 4-HydroxyTamoxifen (4-OH Tam) are greater than 80% at 10
ng/mL.
AFFINIMIP® SPE Tamoxifen
Ordering information:Catalog number: PH101-02 for 25 cartridges, 3mL
PH101-03 for 50 cartridges, 3mL
PH101
LC-UV comparison of urine sample spiked with tamoxifen
and 4-OH tamoxifen (its main metabolite) after extraction
through HLB and AFFINIMIP® SPE
URINE
Comparison between HLB and
AFFINIMIP® SPE Tamoxifen
•Perfect clean-up system for trace analysis of Tamoxifen and its
metabolite in urine
•Unambiguous and reliable analysis
Product Information
Tested matrices : Urine.
Protocol :
Loading : Urine
Washing : Water- Methanol
Elution : Methanol - Acetic acid
Detection : HPLC-UV
Format : 3mL cartridges
Particle diameter range : 25-80 µm
Stability : 3 years
Shipping & Storage : Ambient temperature
For other matrices or formats, please contact us for
technical support
PublicationsInterest of molecularly imprinted polymers in the fight
against doping. Extraction of tamoxifen and its main
metabolite from urine followed by high-performance
liquid chromatography with UV detection. Journal of
Chromatography A, 1196–1197, 81–88, 2008.
www.affinisep.com
AFFINIMIP® SPE Zeranol Residues
Ordering information:Catalog number: FS105-02 for 25 cartridges, 3mL
FS105-03 for 50 cartridges, 3mL
FS105
AFFINIMIP® SPE Zeranol Residues are selective solid-phase extraction cartridges for Zeranol and its
residues from complex matrices such as urine or meat.
Zeranol (or α-Zearalanol) is a potent nonsteroidal growth promoter used extensively in the United
States and Canada to promote rapid growth rates in animals. Nevertheless, its use is banned in
several countries (e.g. European directive 96/22/EC and in China).
These cartridges can extract Zeranol, Zearalenone, β-Zearalanol (Taleranol), α and β-Zearalenol,
Zearalanone and more broadly, Resorcylic acid lactones. The analysis of these compounds in urine
or meat requires a long and tedious pre-treatment sample (several Liquid-Liquid Extractions and
multi SPE steps).
AFFINIMIP®SPE Zeranol Residues provides similar results in an easier manner. These analyses are
sensitive, rapid and reproducible.
CATTLE URINE SPIKED AT 1ng/mL of 6 main Zeranol metabolites
•Extraction of a broad family of Zeranol residues and Resorcylic acid
lactones compounds
•Ready for use and optimized extraction protocols adapted for each
tested matrices
•Very simple and short pre-treatment sample
Product Information
Tested matrices : Urine and plasma.
Protocol :
Loading : loading solution
Washing : Acetonitrile - Water - Acetic
acid
Elution : Methanol - Acetic acid
Detection : HPLC-Fluorescence or LC-MS
Format : 3mL cartridges
Particle diameter range : 25-80 µm
Shipping & Storage : Ambient temperature
For other matrices or formats, please contact
us for technical support
Publications
New technological tools for isolating and
measuring growth promoting agents in edible
tissues and biological fluids, Emmanuelle
Bichon et al. (LABERCA) Conference, RAFA
2011
Quantification of a mixture (Zeranol, Zearalenone,
β-Zearalanol, α and β-Zearalenol and Zearelenone)
in bovine urine spiked at 1ng/mL. Comparison of a
clean-up made with AFFINIMIP® SPE Zeranol
Residues + NH2 cartridges and with IAC clean-up.
(Courtesy of Emmanuelle Bichon - LABERCA)
29
Taleranol-d4/
Zeranol-d4
Taleranol/
Zeranol
α and β-
Zearalenol
Zearalenone
Zearelenone
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AFFINIMIP® SPE Picolinic Herbicides
•Analysis of Picloram, Aminopyralid and Clopyralid
•High recovery yield (>80%)
•Efficient clean-up of Water, Compost and Soil
•Trace analysis by LC-MS/MS
Ordering information:Catalog number: FS115-02 for 25 cartridges, 3mL
FS115-03 for 50 cartridges, 3mL
FS115
AFFINIMIP® SPE Picolinic Herbicides are selective solid-phase extraction cartridges for the
extraction of Picolinic acids based herbicides such as Picloram, Aminopyralid and Clopyralid
from water or compost.
Product information
Tested matrices : compost, water
Protocol :
Loading: Water
Washing : Water - Acetonitrile
Elution : Ethyl acetate /TFA
Detection : LC-MS / UV
Format : 3mL cartridges
Particle diameter range : 25-80 µm
Shipping & Storage : Ambient temperature
For other matrices or formats, please contact
us for technical support
30
Structure of picolinic acid
Analysis of herbicides in compost
Percolation solution
Elution solution
AFFINIMIP® SPE
Efficient clean-upand enrichment
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AFFINIMIP® SPE Glyphosate - AMPA
Glyphosate [(N-phosphonomethyl)glycine] is a non-selective broad-spectrum herbicide that
is extensively used in agriculture. The biodegradation of Glyphosate (GLY) in the
environment leads to aminomethylphosphonic acid (AMPA).
Both molecules are very difficult to analyze due to their high solubility in water and their
insolubility in organic solvents, making liquid extraction difficult.
AFFINIMIP® SPE Glyphosate – AMPA cartridges enable the extraction and analysis of these
compounds.
Glyphosate AMPA
Ordering information:Catalog number: FS113-02 for 25 cartridges
FS113-03 for 50 cartridges
Mean percentage recoveries of FMOC-derivatized Glyphosate and AMPA spikedin Water after AFFINIMIP® SPE Glyphosate- AMPA clean-up and relative standarddeviation (%RSDR) calculated from resultsgenerated under reproducibility conditions(n=4):
Product information
Tested matrices : waters
Protocol :
Loading: Water - Methanol
Washing : Water - Methanol
Elution : HCl solution (100mM)
Detection : LC-MS / Fluo
Format : 3mL cartridges
Particle diameter range : 80-100 µm
Shipping & Storage : Ambient temperature
For other matrices or formats, please contact
us for technical support
n=4Glyphosate AMPA
Recoveries %
87,5 90,2
%RSDR 5,8% 3,8%
•Very simple and fast sample preparation
•Ready for use and optimized protocols
•High recovery yields (>80%)
29
FS113
www.affinisep.com
•A reliable solution for extraction of Catecholamines and its
metabolites
•Good clean-up method
AFFINIMIP® SPE Catecholamines
Free catecholamines
•Dopamine (DA)
•Norepinephrine or Noradrenaline (NA)
•Epinephrine or Adrenaline (A)
LC-UV chromatogram of a serum sample spiked
with Catecholamines at 450nM with (—) and
without (—) AFFINIMIP® SPE Catecholamines
Internal Standard: 4-hydroxy-3-
methoxybenzylamine (HMBA) (Courtesy of B. Claude
and P. Morin, ICOA, Orléans, France)
NA A
NMN
DA HMBA I.S.
Ordering information:Catalog number: DG100-02 for 25 cartridges, 3mL
DG100-03 for 50 cartridges, 3mLDG100-02A for 25 cartridges, 1mLDG100-03A for 50 cartridges, 1mL
DG100
AnalytesRecoveries
%
Dopamine (DA) 67
Noradrenaline (NA) 80
Adrenaline (A) 84
3-Methoxytyramine 102
Normetanephrine (NMN) 106
Recovery yield
AFFINIMIP® SPE Catecholamines are selective solid-phase extraction cartridges that selectively clean
and concentrate the Catecholamines and its amino metabolites from complex matrices such as
Plasma or Serum.
Metabolites
•Metanephrine
•Normetanephrine (NMN)
•3-Methoxytyramine
Product Information
Tested matrices : Plasma and serum
Protocol :
Loading : diluted serum or plasma
Washing : Water - Methanol
Elution : Methanol - Acetic acid
Detection : HPLC-UV Format: 3mL cartridges
Particle diameter range : 25-80 µm
Shipping & Storage : Ambient temperature
For other matrices or formats, please contact
us for technical support
Publications
Analysis of urinary neurotransmitters by
capillary electrophoresis: Sensitivity
enhancement using field-amplified sample
injection and molecular imprinted polymer
solid phase extraction, Bérengère Claude et al.,
Analytica Chimica Acta, 699 (2), 242–248, 2011.
SERUM
32
www.affinisep.com
AFFINIMIP®SPE Metanephrines
•Extraction of Metanephrines by direct percolation of diluted
plasma or serum
Mass chromatogram (SIM+) of plasma spiked with
60nM of Metanephrines after clean-up with
AFFINIMIP® SPE Metanephrines
MN: Metanephrine (m/z=180); NMN:
Normetanephrine (m/z=166); 3MT: 3-
Methoxytyramine (m/z=151) (Courtesy of B. Claude
and P. Morin, ICOA, Orléans, France)
Metanephrine (MN) Normetanephrine (NMN) 3-methoxytyramine (3MT)
Ordering information:Catalog number: DG101-02 for 25 cartridges, 3mL
DG101-03 for 50 cartridges, 3mLDG101-02A for 25 cartridges, 1mLDG101-03A for 50 cartridges, 1mL
DG101
NMN
Inte
nsi
ty
MN
3MT
AFFINIMIP® SPE Metanephrines are selective solid phase extraction cartridges that selectively clean
and concentrate Metanephrines from complex matrices such as Plasma or Serum.
Quantification of free metanephrines in Plasma (Metanephrine, Normetanephrine and 3-
methoxytyramine) is considered to be a highly sensitive test for the diagnosis of Pheochromocytoma
and the follow-up of patients. Free Metanephrines are in a very low concentration in this complex
matrix which makes a reliable determination of the compound an analytical challenge.
Time (min)
Product Information
Tested matrices : Plasma and serum
Protocol :
Loading : diluted serum or plasma
Washing : Water - Methanol
Elution : Methanol - Acetic acid
Detection : HPLC-UV
Format: 3mL cartridges
Particle diameter range : 25-80 µm
Shipping & Storage : Ambient temperature
For other matrices or formats, please contact
us for technical support
Publications
Application note - AFFINIMIP® SPE
Metanephrine in plasma - comparison with
WCX
SERUM
33
www.affinisep.com
AFFINIMIP® SPE Phenolics
•Extraction of a broad range of Phenolic compounds
•Reduction of matrix effects and of most interferents
•Several protocols proposed to purify your analyte
Example of extraction of Phenolic compounds from complex matrix
Before MIP clean-upElution after loading of 10mL of dilutedcomplex matrix spiked with:150ng/mL of analyte
100ng/mL of analyte
Ordering information:Catalog number: FS103-02 for 25 cartridges, 3mL
FS103-03 for 50 cartridges, 3mL
FS103
Phenolic compound
Time (min)
mV
AFFINIMIP® SPE Phenolics are selective solid-phase extraction cartridges
that selectively clean and concentrate a broad range of phenolic
compounds prior to further analysis.
Phenolic compounds are an important family of products found as natural
substances in plants and life sciences or as synthetic products such as drugs.
Phenolics include a large group of several hundred chemical compounds,
characterized by having at least one aromatic ring with one hydroxyl group
attached.
HPLC profile with a fluorescence detection
CLASS SPECIFIC
Product Information
Protocol :
Protocol with an organic loading solution
Loading : Acetonitrile
Washing : Acetonitrile - Methanol
Elution : Methanol - Acetic Acid
Protocol with an hydro-organic loading
solution Loading : Acetonitrile-Water
Washing : Acetonitrile - Water
Elution : Methanol
Format : 3mL cartridges
Particle diameter range : 25-80 µm
Shipping & Storage : Ambient temperature
For other matrices or formats, please contact
us for technical support
34
Example of phenolic moleculeswhich can be purifiedParabensTocopherolsNitrophenolsChlorophenolsCatechinsQuercetinsEtc…
Publications
Molecularly imprinted polymers-liquid
chromatography/fluorescence for the selective
clean-up of hydroxylated polycyclic aromatic
hydrocarbons in soils, O. Baltrons, M. Lopez-
Mesas, Cristina Palet, Franck Lederf and
Florence Portet-Koltalo, Anal. Methods, 2013,
Accepted Manuscript
Application note – AFFINIMIP®SPE Phenolics –
Guaïacol in wine
Application note – AFFINIMIP®SPE Phenolics –
Parabens in cosmetic products
Application note – AFFINIMIP®SPE Phenolics –
Carnosic acid in meat
www.affinisep.com
AFFINIMIP® SPE PRODUCT LIST FOR MYCOTOXINS ANALYSES
35
Products Designation Definition ReferenceNber of
cartridges
MultimycotoxinsAFFINIMIP® SPE
MultimycoLCMSMS
3mL Selective SPE cartridges forMultimycotoxin analyses
FS118-02 25
FS118-03 50
6mL Selective SPE cartridges forMultimycotoxin analyses
FS118-02B 25
FS118-03B 50
Zearalenone and Fumonisins
AFFINIMIP® SPEFumoZON
3mL Selective SPE cartridges for Zearalenone and Fumonisins
FS109-02 25
FS109-03 50
Patulin
AFFINIMIP® SPEPatulin
3mL – 100mg Selective SPE cartridgesfor Patulin
FS102-02 25
FS102-03 50
6mL – 200mg Selective SPE cartridgesfor Patulin
FS102-02B-200mg 25
FS102-03B-200mg 50
AFFINIMIP® SPEPatulin & Pectinase
kit
Kit of 3mL selective SPE cartridges forPatulin + 50mL Pectinase enzymesolution
FS102-02K 25
FS102-03K 50
Kit of 6mL - 200mg selective SPEcartridges for Patulin in dried apple +50mL Pectinase enzyme solution
FS102-02KB-200mg
25
FS102-03KB-200mg
50
Ochratoxin AAFFINIMIP® SPE
Ochratoxin A
3mL Selective SPE cartridges forOchratoxin A
FS101-02 25
FS101-03 50
6mL Selective SPE cartridges forOchratoxin A
FS101-02B 25
FS101-03B 50
DeoxynivalenolAFFINIMIP® SPEDeoxynivalenol
6mL -100mg selective SPE cartridges for Deoxynivalenol mycotoxin in food and babyfood
FS117-02B 25
FS117-03B 50
6mL – 200mg selective SPE cartridgesfor Deoxynivalenol mycotoxin in feed
FS117-02B-200mg 25
FS117-03B-200mg 50
ZearalenoneAFFINIMIP® SPE
Zearalenone3mL Selective SPE cartridges for ZON
FS100-02 25
FS100-03 50
Pectinase 50 mL Pectinase enzyme solution REA-001-50mL
For other formats, please contact usAFFINISEP can provide you with other formats than the one described in the product list. Other
formats available on demand and with an adapted protocol can be :
• different volumes of SPE cartridges (1mL, 3mL, LRC, 60mL, etc… )
• 96 well plates,
• HPLC columns,
• Preparative HPLC columns
• the format adapted to your application and your automate
www.affinisep.com
AFFINIMIP® SPE PRODUCT LIST (MISCELLANEOUS)
Products Designation Definition ReferenceNber of
cartridges
Bisphenol AAFFINIMIP® SPE
Bisphenol A
3mL Selective SPE cartridges for BisphenolA (PP)
FS106-02 25
FS106-03 50
6mL Selective SPE cartridges for BisphenolA (PP)
FS106-02B 25
FS106-03B 50
6mL Selective SPE cartridges for BisphenolA (Glass)
FS106-02G 25
FS106-03G 50
EstrogensAFFINIMIP® SPE
Estrogens
1mL Selective SPE cartridges for EstrogensFS104-02A 25
FS104-03A 50
3mL Selective SPE cartridges for EstrogensFS104-02 25
FS104-03 50
CatecholaminesAFFINIMIP® SPECatecholamines
3mL Selective SPE cartridges forCatecholamines
DG100-02 25
DG100-03 50
1mL Selective SPE cartridges forCatecholamines
DG100-02A 25
DG100-03A 50
MetanephrinesAFFINIMIP® SPEMetanephrines
3mL Selective SPE cartridges forMetanephrines
DG101-02 25
DG101-03 50
1mL Selective SPE cartridges forMetanephrines
DG101-02A 25
DG101-03A 50
Picloram, Aminopyralid,
Clopyralid
AFFINIMIP® SPEPicolinic
Herbicides
3mL Selective SPE cartridges for Picolinicacid based herbicides
FS115-02 25
FS115-03 50
Glyphosate, AMPA
AFFINIMIP® SPEGlyphosate -
AMPA
3mL Selective SPE cartridges forGlyphosate and AMPA
FS113-02 25
FS113-03 50
AmphetaminesAFFINIMIP® SPEAmphetamines
3mL Selective SPE cartridges forAmphetamines derivatives
DG102-02 25
DG102-03 50
ChloramphenicolAFFINIMIP® SPE
Chloramphenicol1mL Selective SPE cartridges forChloramphenicol
FS110-02A 25
FS110-03A 50
TamoxifenAFFINIMIP® SPE
Tamoxifen3mL Selective SPE cartridges for Tamoxifen PH101-02 25
TetracyclinesAFFINIMIP® SPE
Tetracyclines1mL Selective SPE cartridges for Tetracyclines
FS112-02A 25
FS112-03A 50
Zeranol ResiduesAFFINIMIP® SPE
Zeranol Residues3mL Selective SPE cartridges for ZeranolResidues
FS105-02 25
FS105-03 50
PhenolicsAFFINIMIP® SPE
Phenolics3mL Selective SPE cartridges for Phenoliccompounds
FS103-03 50
34
www.affinisep.com 38
The choice of the suitable AttractSPETM columns can be done by using the followingmethod:- Check if a method already exists on the application notebook of our website-Determination of the nature of the analyte (neutral, acid, base)-Determination of the pKa-Choice of the AttractSPETM columns by using the following chart and application of thegeneral protocol described on the instruction sheet- Analysis of the recovery yields
Sample preparation
Conditioning and equilibration
loading of the solution
Washing with Formic acid 2%
2mL Methanol
2mL 5% NH4OH /Methanol
Sample preparation
Conditioning and equilibration
loading of the solution
Washing with NH4OH 5%
2mL Methanol
2mL 5% Formic acid /Methanol
Sample preparation
Conditioning and equilibration
loading of the solution
Washing with Methanol/ Water
2mL Methanol
Neutral compounds
Strong basesBasesStrong acids Acids
AttractSPETM
WAXStrong acids
pKa<1
AttractSPETM
SCXBases
pKa 2-10
AttractSPETM
WCXStrong Bases
pKa >10
AttractSPETM
SAXAcids pKa 2-8
AttractSPETM
DVBNeutral
hydrophobic
AttractSPETM
W/O - HLBNeutral
AttractSPETM are based on polymeric sorbents dedicated to sample clean-up for the
extraction of compounds from complex matrices. These cartridges contain polymeric
sorbents which support the six common retention mechanisms used in today’s analytical
laboratory:
•Hydrophilic Lipophilic Balance (AttractSPETM W/O - HLB)
•Mixed-mode Strong Cation Exchange (AttractSPETM SCX)
•Mixed-mode Weak Cation Exchange (AttractSPETM WCX)
•Mixed-mode Strong Anion Exchange (AttractSPETM SAX)
•Mixed-mode Weak Anion Exchange (AttractSPETM WAX)
•Reversed phase (AttractSPETM DVB)
AttractSPETM cartridges provide the advantages of robustness, simplified method
development, wide applicability and are not affected by drying out. The combination of the
water-wettable optimised surface chemistry, high surface area and pH stability ensures high
reproducible recoveries for a wide range of analytes.
POLYMERIC AttractSPETM CARTRIDGES
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AttractSPETM W/O - HLB is an uncharged Hydrophilic and Lipophilic sorbent interacting with both, hydrophilic and hydrophobic interactions.
AttractSPETM W/O - HLB particularly suits for
the extraction of a wide range of analytes
(polar, apolar, neutral, acid, basic…)
Product Information
Protocol :
Loading : Pre-treated sampleWashing: Methanol - WaterElution: Methanol
Format : see product list
Particle diameter range : 40 µm
Pore size: 70 ÅSurface area: 800 m2/g
Shipping & Storage : Ambient temperature
POLYMERIC AttractSPETM CARTRIDGES
Reversed phase SPE for extraction of
hydrophobic analytes
AttractSPETM DVB is a polystyrene-
divinylbenzene copolymer presenting a high
hydrophobicity used as a reversed-phase.
AttractSPETM DVB particularly suits for the
extraction of hydrophobic analytes.
Product Information
Format : see product list
Particle diameter range : 40 µm
Pore size: 60 ÅSurface area: 600 m2/g
Shipping & Storage : Ambient temperature
39
AttractSPETM W/O - HLB
AttractSPETM DVB
Mixed-mode SPE for extraction of strong acid
analytes
AttractSPETM WAX is a weak anion exchange
sorbent interacting with the analytes via a
mixed mode retention mechanism, ion
exchange with weak basic functional groups
and reversed phase.
AttractSPETM WAX particularly suits for the
extraction of strong acids.
Product Information
Protocol :
Loading : Neutral to Acidified sampleWashing 1: Formic acid – WaterWashing 2 or Elution 1: Methanol Elution 2: Ammonium hydroxide – Methanol
Format : see product list
Pore size: 60 ÅSurface area: 650 m2/gIonic capacity: 0.5 meq/g
Particle diameter range : 40 µm
Shipping & Storage : Ambient temperature
AttractSPETM WAX
www.affinisep.com
Mixed-mode SPE for extraction of strong basic
analytes
AttractSPETM WCX is a weak cation exchange
sorbent interacting with the analytes via a
mixed mode retention mechanism, ion
exchange with weak acid functional groups and
reversed phase.
AttractSPETM WCX particularly suits for the
extraction of strong bases and quaternary
amines.
POLYMERIC AttractSPETM CARTRIDGES
Product Information
Protocol :
Loading: Neutral to Basic sampleWashing 1: Ammonium hydroxide - WaterWashing 2: MethanolElution: Formic acid - Methanol
Format : see product list
Pore size: 70 ÅSurface area: 850 m2/gIonic capacity: 0.77meq/g
Particle diameter range : 40 µm
Shipping & Storage : Ambient temperature
40
AttractSPETM WCX
Mixed-mode SPE for extraction of weak acid
analytes
AttractSPETM SAX is a strong anion exchange
sorbent interacting with the analytes via a
mixed mode retention mechanism, ion
exchange with strong basic functional groups
and reversed phase.
AttractSPETM SAX particularly suits for the
extraction of weak acids.
Product Information
Protocol :
Loading: Neutral to Basic sampleWashing 1: Ammonium hydroxide - WaterWashing 2: MethanolElution: Formic acid - Methanol
Format : see product list
Particle diameter range : 40 µm
Pore size: 60 ÅSurface area: 600 m2/gIonic capacity: 0.3 meq/g
Shipping & Storage : Ambient temperature
AttractSPETM SAX
Mixed-mode SPE for extraction of weak basic
analytes
AttractSPETM SCX is a strong cation exchange
sorbent interacting with the analytes via a
mixed mode retention mechanism, ion
exchange with strong acid functional groups
and reversed phase.
AttractSPETM SCX particularly suits for the
extraction of weak bases.
Product Information
Protocol :
Loading : Neutral to Acidified sampleWashing 1: Formic acid - WaterWashing 2 or Elution 1: Methanol Elution 2: Ammonium hydroxide – Methanol
Format : see product list
Pore size: 60 ÅSurface area: 600 m2/gIonic capacity: 1meq/g
Particle diameter range : 40 µm
Shipping & Storage : Ambient temperature
AttractSPETM SCX
www.affinisep.com
For other formats, please contact usAFFINISEP can provide you with other formats than the one described in the product list. Other
formats available on demand and with an adapted protocol can be :
• different volumes of SPE cartridges (1mL, 3mL, LRC, 60mL, etc… )
• 96 well plates,
• HPLC columns,
• Preparative HPLC columns
• the format adapted to your application and your automate
POLYMERIC AttractSPETM PRODUCT LIST
41
Cartridgesformat,Sorbentamount
Nber /boxAttractSPETM
W/O - HLBAttractSPETM
SCXAttractSPETM
WCXAttractSPETM
SAXAttractSPETM
WAXAttractSPETM
DVB
1mL, 30mg 100 HLB-100.S.1.30 SCX-100.S.1.30 WCX-100.S.1.30 SAX-100.S.1.30
WAX-100.S.1.30
DVB-100.S.1.30
3mL, 60mg50 HLB-50.S.3.60 SCX-50.S.3.60 WCX-50.S.3.60 SAX-50.S.3.60 WAX-50.S.3.60 DVB-50.S.3.60
100 HLB-100.S.3.60 SCX-100.S.3.60 WCX-100.S.3.60 SAX-100.S.3.60WAX-
100.S.3.60DVB-100.S.3.60
6mL, 200mg
25 HLB-25.S.6.200 SCX-25.S.6.200 WCX-25.S.6.200 SAX-25.S.6.200WAX-
25.S.6.200DVB-25.S.6.200
50 HLB-50.S.6.200 SCX-50.S.6.200 WCX-50.S.6.200 SAX-50.S.6.200WAX-
50.S.6.200DVB-50.S.6.200
100HLB-
100.S.6.200SCX-100.S.6.200
WCX-100.S.6.200
SAX-100.S.6.200
WAX-100.S.6.200
DVB-100.S.6.200
6mL, 500mg
25 HLB-25.S.6.500 SCX-25.S.6.500 WCX-25.S.6.500 SAX-25.S.6.500WAX-
25.S.6.500DVB-25.S.6.500
50 HLB-50.S.6.500 SCX-50.S.6.500 WCX-50.S.6.500 SAX-50.S.6.500WAX-
50.S.6.500DVB-50.S.6.500
100HLB-
100.S.6.500SCX-100.S.6.500
WCX-100.S.6.500
SAX-100.S.6.500
WAX-100.S.6.500
DVB-100.S.6.500
96 wellsPlate, 30mg
1 HLB-1.96W.30 SCX-1.96W.30 WCX-1.96W.30 SAX-1.96W.30 WAX-1.96W.30 DVB-1.96W.30
Reversible 0.7mL, 30mg
25HLB-
25.REV.1.N10SCX-
25.REV.1.N10WCX-
25.REV.1.N10SAX-
25.REV.1.N10WAX-
25.REV.1.N10DVB-
25.REV.1.N10
50HLB-
50.REV.1.N10SCX-
50.REV.1.N10WCX-
50.REV.1.N10SAX-
50.REV.1.N10WAX-
50.REV.1.N10DVB-
50.REV.1.N10
Reversible 0.7mL, 100mg (200mg for DVB)
25 HLB-25.REV.1.F SCX-25.REV.1.F WCX-25.REV.1.F SAX-25.REV.1.FWAX-
25.REV.1.FDVB-
25.REV.1.F
50 HLB-50.REV.1.F SCX-50.REV.1.F WCX-50.REV.1.F SAX-50.REV.1.FWAX-
50.REV.1.FDVB-
50.REV.1.F
Reversible 2mL, 225mg
25HLB-
25.REV.2.N10SCX-
25.REV.2.N10WCX-
25.REV.2.N10SAX-
25.REV.2.N10WAX-
25.REV.2.N10
50HLB-
50.REV.2.N10SCX-
50.REV.2.N10WCX-
50.REV.2.N10SAX-
50.REV.2.N10WAX-
50.REV.2.N10
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SilactSPETM Silica-Based Sorbents
SilactSPETM C18 : Strongly hydrophobic and
non-polar sorbent
It was recently developed as an innovative C18
phase characterized by a homogeneous
coverage of the silane on the surface.
SilactSPETM C18 particularly suits for the
extraction of acidic, neutral and basic
compounds from aqueous solutions, various
organic compounds from water, and drugs and
metabolites from physiological fluids.
Product Information
Loading : 17 % C
Endcapping : Yes
Silica type : 60 Å, 500 m2/g, 40-63 µm
SilactSPETM C8: Moderately hydrophobic and
non-polar sorbent
Sorbent C8 is more selective than Sorbent C18
for big compounds such as PAH, vitamin D, and
oils as well as greasy compounds.
SilactSPETM C8 particularly suits for the extraction of extremely non-polar compounds.
Product Information
Loading : 12 % C
Endcapping : Yes
Silica type : 60 Å, 500 m2/g, 40-63 µm
44
SilactSPETM Phenyl : Moderately hydrophobic
and non-polar sorbent
it particularly suits for the extraction of non-
polar compounds with different selectivities
through π-π interactions including aromatic
compounds and other non-polar phases.
Product Information
Loading : 9 % C
Endcapping : Yes
Silica type : 60 Å, 500 m2/g, 40-63 µm
Ordering information: References and formatson the product list
SilactSPETM Silica : Most polar sorbent
It presents a slightly acidic character and is
used to extract various compounds from non-
polar solvents through hydrogen bonding.
Product Information
Silica type : 60 Å, 500 m2/g, 40-63 µm
SilactSPETM Cyano : Moderately polar sorbent
It is used as a normal phase (less polar
compared to silica).
SilactSPETM Cyano particularly suits for the
extraction of acidic, basic and neutral
compounds from aqueous solutions. It is also
used as a reversed-phase (less hydrophobic
than C8 and C18).
Product Information
Loading : 7 % C
Endcapping : Yes
Silica type : 60 Å, 500 m2/g, 40-63 µm
SilactSPETM products are Silica-based sorbents SPE cartridges available in different formats with
different sorbents, and in bed weights up to 10 grams (>10 g are also available in SilactSPE OT
formats)
www.affinisep.com
SilactSPETM Silica-Based Sorbents
45
SilactSPETM Alumina-Acidic, Neutral and Basic:
Alumina can present either cationic, neutral
and acidic character. It is used in a similar
fashion as for the SilactSPE Silica. The
difference is that Alumina is more stable at
high pH than silica.
SilactSPETM Alumina particularly suit for the
retention of aromatic compounds, aliphatic
amines and compounds containing
electronegative functions.
Product Information
Alumina type : 60 Å, 0.9 g/mL, 50-200 µm
Ordering information: References and formatson the product list
SilactSPETM Amine: Weak anion exchanger
silica-based sorbent
SilactSPETM Amino avoids irreversible retention
of acidic molecules (pKa < 3) and particularly
suits for the separation of peptides, drugs and
metabolites from physiological fluids, poly- and
monosaccharides and structural isomers.
Product Information
Loading : 1.6 mmol/g
Endcapping : Yes
Silica type : 60 Å, 500 m2/g, 40-63 µm
SilactSPETM Carbonate: General base quencher
SilactSPE Carbonate is the silica-bound
equivalent of tetramethyl ammonium
carbonate and is used as a general base to
quench a reaction, free base amines in their
ammonium salt form and to scavenge acids,
boronic acids and acidic phenols including
HOBt.
Product Information
SilactSPETM PSA: Weak anion exchanger silica-
based sorbent
Less polar sorbent than SilactSPETM Amineused for its replacement with analytes that aretoo strongly retained on an amine phase.
Product Information
Endcapping : Yes
Silica type : 60 Å, 500 m2/g, 40-63
µm
SilactSPETM Florisil and Florisil PR (MgO3Si) :
Polar sorbent
They present a basic character used to extract
non-polar to moderately polar compounds
from non-polar solvents.
They particularly suit for the retention of
chlorinated pesticides, polychlorinated
biphenyl (PCB’s) and polysaccharides due to
the magnesium ion.
Product Information
Florisil type : 75-150 µm
Florisil PR type : 150-200 µm
www.affinisep.com
For other formats, please contact usAFFINISEP can provide you with other formats than the one described in the product list. Other
formats available on demand and with an adapted protocol can be :
• different volumes of SPE cartridges (1mL, 3mL, LRC, 60mL, etc… )
• 96 well plates,
• HPLC columns,
• Preparative HPLC columns
• the format adapted to your application and your automate
46
Non polar sorbents Polar sorbents
Cartridgesformat,Sorbentamount
#/box
SilactSPETM
C18SilactSPETM
C8SilactSPETM
PhenylSilactSPETM
SilicaSilactSPETM
CyanoSilactSPETM
FlorisilSilactSPETM
Florisil PR
1mL, 50mg 100 C18-100.S.1.50 C8-100.S.1.50 Phe-100.S.1.50 Si-100.S.1.50 CN-100.S.1.50 Flo-100.S.1.50
FloPR-100.S.1.50
1mL, 100mg 100C18-
100.S.1.100C8-100.S.1.100
Phe-100.S.1.100
Si-100.S.1.100CN-
100.S.1.100Flo-
100.S.1.100FloPR-
100.S.1.100
3mL, 200mg 50C18-
50.S.3.200C8-
50.S.3.200Phe-
50.S.3.200Si-
50.S.3.200CN-
50.S.3.200Flo-
50.S.3.200FloPR-
50.S.3.200
3mL, 500mg 50C18-
50.S.3.500C8-
50.S.3.500Phe-
50.S.3.500Si-
50.S.3.500CN-
50.S.3.500Flo-
50.S.3.500FloPR-
50.S.3.500
6mL, 500mg50
C18-50.S.6.500
C8-50.S.6.500
Phe-50.S.6.500
Si-50.S.6.500
CN-50.S.6.500
Flo-50.S.6.500
FloPR-50.S.6.500
6mL, 1g 50C18-
50.S.6.1000C8-
50.S.6.1000Phe-
50.S.6.1000Si-
50.S.6.1000CN-
50.S.6.1000Flo-
50.S.6.1000FloPR-
50.S.6.1000
6mL, 2g50
C18-50.S.6.2000
C8-50.S.6.2000
Phe-50.S.6.2000
Si-50.S.6.2000
CN-50.S.6.2000
Flo-50.S.6.2000
FloPR-50.S.6.2000
12mL, 2g 20C18-
20.S.12.2000C8-
20.S.12.2000Phe-
20.S.12.2000Si-
20.S.12.2000CN-
20.S.12.2000Flo-
20.S.12.2000FloPR-
20.S.12.2000
Reversible 0.7mL, 200mg
25C18-
25.REV.1.200C8-
25.REV.1.200Phe-
25.REV.1.200Si-
25.REV.1.200CN-
25.REV.1.200Flo-
25.REV.1.200FloPR-
25.REV.1.200
Reversible 2mL, 750mg
25C18-
25.REV.2.750C8-
25.REV.2.750Phe-
25.REV.2.750Si-
25.REV.2.750CN-
25.REV.2.750Flo-
25.REV.2.750FloPR-
25.REV.2.750
SilactSPETM PRODUCT LIST
www.affinisep.com
For other formats, please contact usAFFINISEP can provide you with other formats than the one described in the product list. Other
formats available on demand and with an adapted protocol can be :
• different volumes of SPE cartridges (1mL, 3mL, LRC, 60mL, etc… )
• 96 well plates,
• HPLC columns,
• Preparative HPLC columns
• the format adapted to your application and your automate
47
Polar sorbentsOthers sorbents
Cartridgesformat,Sorbent amount
#/boxSilactSPETM
Alumina Acidic
SilactSPETM
Alumina Neutral
SilactSPETM
Alumina BasicSilactSPETM
AmineSilactSPETM
PSASilactSPETM
Carbonate
1mL, 50mg 100 AluA-100.S.1.50 AluN-100.S.1.50 AluB-100.S.1.50 NH2-100.S.1.50 PSA-100.S.1.50 CO3-100.S.1.50
1mL, 100mg 100AluA-
100.S.1.100AluN-
100.S.1.100AluB-
100.S.1.100NH2-
100.S.1.100PSA-
100.S.1.100CO3-
100.S.1.100
3mL, 200mg 50AluA-
50.S.3.200AluN-
50.S.3.200AluB-
50.S.3.200NH2-
50.S.3.200PSA-
50.S.3.200CO3-
50.S.3.200
3mL, 500mg 50AluA-
50.S.3.500AluN-
50.S.3.500AluB-
50.S.3.500NH2-
50.S.3.500PSA-
50.S.3.500CO3-
50.S.3.500
6mL, 500mg50
AluA-50.S.6.500
AluN-50.S.6.500
AluB-50.S.6.500
NH2-50.S.6.500
PSA-50.S.6.500
CO3-50.S.6.500
6mL, 1g 50AluA-
50.S.6.1000AluN-
50.S.6.1000AluB-
50.S.6.1000NH2-
50.S.6.1000PSA-
50.S.6.1000CO3-
50.S.6.1000
6mL, 2g50
AluA-50.S.6.2000
AluN-50.S.6.2000
AluB-50.S.6.2000
NH2-50.S.6.2000
PSA-50.S.6.2000
CO3-50.S.6.2000
12mL, 2g 20AluA-
20.S.12.2000AluN-
20.S.12.2000AluB-
20.S.12.2000NH2-
20.S.12.2000PSA-
20.S.12.2000CO3-
20.S.12.2000
Reversible 0.7mL, 200mg
25AluA-
25.REV.1.200AluN-
25.REV.1.200AluB-
25.REV.1.200NH2-
25.REV.1.200PSA-
25.REV.1.200CO3-
25.REV.1.200
Reversible 2mL, 750mg
25AluA-
25.REV.2.750AluN-
25.REV.2.750AluB-
25.REV.2.750NH2-
25.REV.2.750PSA-
25.REV.2.750CO3-
25.REV.2.750
SilactSPETM PRODUCT LIST
www.affinisep.com 50
Product Product referenceNber
columnI.D. (mm)
Lenght (mm)
On-line AttractSPETM
W/O - HLB columns
OnlineSPE-HLB-1.2.20 1 2.1 20
OnlineSPE-HLB-1.5.20 1 4.6 20
For other on-line SPE products, please contact usAFFINISEP can provide you with on-line SPE of all products on demand
On-line SPE columns for hydrophilic – hydrophobic polymers
ON-LINE SPE PROCEDURE STEPS
SPE technique can be coupled on-line to HPLC for a high sensitivity or for limited amount of
sample. An on-line SPE column containing the SPE sorbents is coupled within the injection
loop of a six-port rotary valve. The on-line SPE column is reusable.
A three steps process is used:
1- Sample injection: The valve is configured with the injector directly in contact with the on-
line SPE column. The sample is injected and goes through the on-line SPE column where the
analytes remain.
2- Washing: a solution is used to wash out most interferences.
3- Analysis: The valve is switched. The analytes are eluted out of the sorbent by the LC
mobile phase and transfered into the analytical column for their analyses.
ON-LINE SPE – DESCRIPTION AND PRODUCT LIST
Sample injection and washing (1 and 2) Sample elution and analysis (step 3)
Analytical column
Detector
HPLC Pump
SPE column
Sample injectorWaste
Analytical column
Detector
HPLC Pump
SPE column
Sample injectorWaste
Flow Flow
Product Product referenceNber
columnI.D. (mm)
Lenght (mm)
On-line AFFINIMIP® PHENOLICS columns
OnlineSPE-PHE-1.2.20 1 2.1 20
OnlineSPE-PHE-1.5.20 1 4.6 20
On-line SPE columns for AFFINIMIP® Phenolics
www.affinisep.com 52
QuEChERS is a sample pretreatment initially developed by Michelangelo Anastassiades and
Steven Lehotay is mainly used for the analysis of multiple pesticides into fruits and
vegetables. It is the acronym of Quick, Easy, Cheap, Effective, Rugged ans Safe. Three main
methods are currently used the original method, the European standardized method EN
15662 and the AOAC official method 2007.01. Each method required an extraction process
with salts and a clean-up process with SPE cartridges or by dispersive SPE.
QcleanupTM - Description
AFFINISEP supplies all products required to carry out QuEChERS according to AOAC or CENincluding dispersive SPE products or SPE cartridges.
www.affinisep.com 53
QcleanupTM - PRODUCT LIST
QcleanupTM EXTRACTION SALTS
QuEChERS methods DescriptionPouches /
boxProduct
reference
Original method4g MgSO4
1g NaCl50 EXT.ORL.50
EN 15662
1g Trisodium citrate Dihydrate0.5g Disodium hydrogencitrate
sesquihydrate1g NaCl and 4g MgSO4
50 EXT.EN.50
AOAC 2007.01 1.5g Sodium Acetate and 6g MgSO4 50 EXT.AOAC.50
QcleanupTM DISPERSIVE SPE PRODUCTS
Method Description Nber/box Product reference
For General Fruits & Vegetables
EN 15662150mg MgSO4 + 25mg PSA 100 tubes of 2mL dSPE.EN.GFV.100.2
900mg MgSO4 + 150mg PSA 50 tubes of 15mL dSPE.EN.GFV.50.15
AOAC 2007.01
150mg MgSO4 + 50mg PSA 100 tubes of 2mL dSPE.AOAC.GFV.100.2
1200mg MgSO4 + 400mg PSA 50 tubes of 15mL dSPE.AOAC.GFV.50.15
For Pigmented Fruits & Vegetables
EN 15662150mg MgSO4 + 25mg PSA + 2.5mg CB 100 tubes of 2mL dSPE.EN.PFV.100.2
900mg MgSO4 + 150mg PSA + 15mg CB 50 tubes of 15mL dSPE.EN.PFV.50.15
AOAC 2007.01
150mg MgSO4 + 50mg PSA + 50mg CB 100 tubes of 2mL dSPE.AOAC.PFV.100.2
1200mg MgSO4 + 400mg PSA + 400mg CB 50 tubes of 15mL dSPE.AOAC.PFV.50.15
For Highly Pigmented and Fatty Fruits & Vegetables
EN 15662150mg MgSO4 + 25mg PSA + 7.5mg CB 100 tubes of 2mL dSPE.EN.HPFV.100.2
900mg MgSO4 + 150mg PSA + 45mg CB 50 tubes of 15mL dSPE.EN.HPFV.50.15
AOAC 2007.01
150mg MgSO4 + 50mg PSA + 50mg CB +50mg C18
100 tubes of 2mL dSPE.AOAC.HPFV.100.2
1200mg MgSO4 + 400mg PSA + 400mg CB + 400mg C18
50 tubes of 15mL dSPE.AOAC.HPFV.50.15
For Fatty and waxed Fruits & Vegetables
EN 15662150mg MgSO4 + 25mg PSA + 25mg C18 100 tubes of 2mL dSPE.EN.FWFV.100.2
900mg MgSO4 + 150mg PSA + 150mg C18 50 tubes of 15mL dSPE.EN.FWFV.50.15
AOAC 2007.01
150mg MgSO4 + 50mg PSA + 50mg C18 100 tubes of 2mL dSPE.AOAC.FWFV.100.2
1200mg MgSO4 + 400mg PSA + 400mg C18 50 tubes of 15mL dSPE.AOAC.FWFV.50.15
QcleanupTM products for dispersive SPE are mixtures of powder in 2mL or 15mL centrifugation tubesfor main scenarios encountered during pesticide analyses. This mixture contains magnesium sulfateanhydrous (MgSO4), primary secondary amine (PSA), carbon black (CB) or C18.
QcleanupTM extraction salts are the three main salts mixtures used in QuEChERS method.
www.affinisep.com
Mycotoxines ProductConcentration
(µg/mL) Volume Référence
Aflatoxins
Aflatoxin B1 2 1mL REA-AFLB1-1ML
Aflatoxin B2 0.5 1mL REA-AFLB2-1ML
Aflatoxin G1 2 1mL REA-AFLG1-1ML
Aflatoxin G2 0.5 1mL REA-AFLG2-1ML
Mixture Aflatoxins: B1-B2-G1-G2 2-0.5-2-0.5 1mL REA-MIXAFL-1ML
Aflatoxin M1 0.5 1mL REA-AFLM1-1ML
Ochratoxin Ochratoxin A 100 1mL REA-OTA-1ML
Patulin Patulin 100 1mL REA-PAT-1ML
Trichothecenes AT2 Toxin 100 1mL REA-T2-1ML
HT-2 Toxin 100 1mL REA-HT2-1ML
Trichothecenes B Deoxynivalenol (DON) 100 1mL REA-DON-1ML
Zearalenone Zearalenone 100 1mL REA-ZON-1ML
Fumonisines
Fumonisine B1 50 1mL REA-FB1-1ML
Fumonisine B2 50 1mL REA-FB2-1ML
Mixture Fumonisine: B1-B2 50 1mL REA-MIXFB-1ML
MYCOTOXINS STANDARDS
56
AFFINISEP proposes the following Mycotoxins standards for mycotoxins analyses :
Stability: 1 year
Storage: 4°C
Mycotoxins are toxic byproducts of mold infestations which can
contaminate food. To protect consumers from the health risks,
most countries have adopted regulations to limit exposure to
mycotoxins.
Our mycotoxins standards are accurately prepared solutions of
a mycotoxin or a mixture of mycotoxins to a determined
concentration. These standards can be used for the
implementation of the calibration curve as well as for the
development of a new analytical methods. They are supplied
with a certificate of analysis.
MYCOTOXINS STANDARDS – Product List
Please check our website for an updated list of standard solutions
www.affinisep.com
Manifold ACC-MAN1 Like all chromatography techniques, Use of SPE cartridges needs
a precise control of flow rate for maintaining reproducible
extractions. Solid Phase extraction Vacuum Manifold allows you
to control the flow and to process up to 12 (12-port version) or
24 (24-port version) AFFINIMIP® SPE samples simultaneously, to
gain significantly time during sample preparation steps.
SPE Adapter &
Reservoir kit
ACC-AR1 Tube adapters serve to pile one SPE tube on top of another to
provide different selectivities. A larger empty syringe barrel can
be stacked on top of a smaller SPE tube to act as a larger load
reservoir. Or, they can serve as an adapter for positive pressure
methods (e.g. from a syringe or air/ N2 line).
Mini-Vap ACC-VAP1 The 6-Port Mini-Vap concentrator/evaporator processes six vials
at one time. The Mini-Vap includes a needle valve for fine
metering of air or nitroge n drying gas.
Mini PUMP ACC-PUMP Mini diaphragm vacuum pump for solid phase extraction
experiments
Portable
5.5L/min
~120 torr vacuum
Oil-free
portable
Vacuum pump trap ACC-TRAP SPE Vacuum pump trap kit
Installed between the manifold and the vacuum pump, it
collects all liquids that are aspirated preventing contamination
of the vacuum pump with a capacity of 1L.
AFFINISEP proposes the complete set of equipments required to carry out SPE experiments:
SPE ACCESSORIES
58
SPE Accessories Designation Definition Reference
Manifold SPE Vaccum Manifold 12-port model ACC-MAN1
SPE Adapter & Reservoir
kitSPE Adapter & Reservoir kit
Kit of 12 reservoirs 60ml and adapters
for use with 1,3 & 6 mL cartridgesACC-AR1
Mini-VapMini
Evaporator/Concentrator
6 port Mini-Vap
Evaporator/Concentrator for use with 1
to 250mL containers
ACC-VAP1
Mini PUMP Mini vacuum pumpLaboport diaphragm vacuum mini
pump, 5.5L/minACC-PUMP
Vacuum pump trap SPE Vacuum pump trap kit 1L trap kit ACC-TRAP
SPE ACCESSORIES – Product list
www.affinisep.com
AFFINISEP offers full services for the design and synthesis of polymers complying with your
specifications.
With fully integrated technology platforms in organic, polymer and analytical chemistry,
AFFINISEP has been partner in more than 40 projects and helps its customers by innovative
solutions for their complexes challenges.
AFFINISEP has developed a library of monomers giving a family of selective stationary phases
based on its proprietary technology, which have shown a strong potential for the extraction,
purification and detection of various compounds.
Did you know ?Molecularly imprinted polymer (MIP) is a polymer with a «memory» of the shapeand the functional groups of a target molecule. This material is designed in orderto recognize selectively this molecule, even in the presence of compounds withstructure and functionality similar to those of this molecule. High molecularrecognition properties can be achieved with these MIPs for a variety of moleculesand can be useful for its extraction, purification and detection.
60
CUSTOM-MADE PRODUCTS & SERVICES
Why trusting AFFINISEP? AFFINISEP supplies
• The most comprehensive bank of sorbents
•From Silica to Polymers
•From conventional to very selective
•Reversed-phase, ionic exchange, MIPs etc…
•Our Expertise on MIP, sample preparation, SPE protocol and detection
kit development
•Quick and efficient development
•Reactivity
Custom-made
solutions for:
SPE for analytical
applications
Purifications SPE for
radiopharma
Molecularly imprinted polymers
development
Custom-made
formats
www.affinisep.com
If you need the development of new polymer for your application, please send us an email
to [email protected] or describe your needs using
http://www.polyintell.com/services/request-service-online/. You can describe your
application and our scientists will shortly evaluate your queries before contacting you as
soon as possible.
61
STEP 1
STEP 2
STEP 3
Procedure for custom-made polymer phase
CustomerFe
edb
ack
and
op
tim
isat
ion
•Screening
• Elaboration ofapplication protocol• Delivery ofsamples/ prototypesto customer forvalidation
• Scale-up• QC report
Bulk material, SPE cartridges or HPLCcolumns
Step 1: We offer you a screening of our library
which consists of several hundreds AFFINISEP
phases to find the suitable one for your
separation problem. The knowledge of the
structure of the target substance, its
functional groups and the solubility data give
us valuable hints for the choice of the
screening phases.
Step 2: For the selected phase, a protocol is
implemented for your application. Then
samples and / or prototypes are delivered to
you for testing, evaluation and validation.
Step 3: When the selected phase suits your
application and has been validated, a scale-up
is planned. A QC report is delivered with the
product. The format of the product is
correlated to your application and can be bulk
material, SPE cartridges, HPLC columns etc…
No matter if we run a short term project (2-3 days) or long term project ( 4 to 6 months) we
always follow a well-established procedure. The following scheme describes an example of a
procedure for the development of a custom-made product based on our customer’s
requirements.
After signing of Confidential Disclosure Agreements (CDA), key data are exchanged. A
quotation based on our experience in separation science including a process sheet is
developed. During the whole procedure, a permanent feedback is established with you.
PROJECT DEVELOPMENT
About AFFINISEPAFFINISEP is a worldwide expert in purification and sample preparation applications as well as for
the design and the development of intelligent polymers with Molecularly Imprinted Polymers (MIP).
AFFINISEP is dedicated to the development of analytical applications in various fields such as water,
biological fluids, food and feed analysis with a complete set of products and services for sample
preparation:
SPE cartridges
On-line SPE columns
QuEChERS
Standards
Our mission is to develop and market innovative products of high value to customers by a practical
contribution to their work. By offering you a most comprehensive range of solid phase extraction
products, AFFINIMIP® SPE products based on molecularly imprinted polymers, AttractSPETM a range
of polymeric phases and SilactSPETM Silica based products, associated reagents, QuEChERS and
small equipment, the analytical chemists can find any solution for sample preparation, selective
extraction and sample clean-up needs in various sectors: food and feed safety and quality,
pharmaceutical R&D and quality control, clinical diagnosis, environment and doping.
Furthermore, by exploiting our library of innovative polymers and our know-how in chromatography
and solid phase extraction, we have a strong capacity to adapt these polymers to meet any specific
requirements and to solve unsatisfied purification and extraction needs.
Numerous documents related to our products (Application notebooks, publication references,
posters, catalog for different applications…) can be found on our website www.affinisep.com.
For any order, please, choose one of the following ways:
By mail:
AFFINISEP
Pharma Parc II
Voie de l’innovation
27100 Val de Reuil, FRANCE
By fax: +33 (0)2 32 59 61 01
By email: [email protected]
On-line by E-commerce: To save your time and a quicker delivery,
order via our web site www.affinisep.com
Information about new products or new matrices:
Newsletters
Stay up-to-date with the news on AFFINISEP’s latest innovations by subscribing to our newsletter,
delivering new information straight to your inbox.
ORDERING INFORMATION
2181 N Tracy Blvd Ste 202, Tracy, CA 95376 USAphone: 1-347-688-4752 [email protected] www.columnnex.com
Columnex LLC
Columnex LLC represents all Affinisep products sold in America