sample IDgene cHGVS pHGVS exon/intronallele frequencyfunctionPE270 MED12 c.4850C>G p.A1617G EX35 33.6% missensePE274 NSD1 c.7696A>T p.T2566S EX23 6.5% missensePE252 TGFBR2 c.449A[10>11] p.P154Afs*3 EX4 21.4% frameshiftPE331 JAK3 c.2062A>T p.I688F EX16 1.1% missensePE370 NFE2L2 c.242G>A p.G81D EX2 3.3% missensePE418 MAGI2 c.1244G[3>2] p.D416Mfs*5 EX9 5.2% frameshiftPE457 KDM6A c.225G[2>1] p.A76Lfs*8 EX2 8.9% frameshiftPE582 EGFR c.2573T>G p.L858R EX21 28.4% missensePE411 SOX2 c.871A>G p.R291G EX1E 8.2% missensePE660 EGFR c.2573T>G p.L858R EX21 3.6% missensePE733 IL7R c.832G>A p.D278N EX7 12.0% missensePE689 GAB2 c.862G>T p.D288Y EX4 1.4% missensePE479 BCL10 c.129A[8>7] p.I46Yfs*24 EX2 3.8% frameshiftPE478 KRAS c.35G>A p.G12D EX2 4.1% missensePE768 EGFR c.2235_2249delGGAATTAAGAGAAGCp.E746_A750del EX19 3.8% cds-delPE703 FGFR2 c.626T>C p.V209A EX6 18.2% missensePE739 TSHR c.1139C>T p.A380V EX10E 29.6% missensePE902 TP53 c.916C>T p.R306* EX8 3.6% nonsensePE892 CDH23 c.9724T>A p.S3242T EX69 5.4% missensePE869 LRP1B c.9230G>C p.G3077A EX58 7.4% missensePE897 ROBO3 c.2990_2991insA p.I999Nfs*22 EX21 8.4% frameshiftPE231 TP53 c.533A>C p.H178P EX5 31.8% missensePE255 FBXW7 c.1514G>A p.R505H EX10 0.4% missensePE252 MLL c.9688C>A p.Q3230K EX27 43.3% missensePE346 EGFR c.2573T>G p.L858R EX21 29.8% missensePE410 EGFR c.2152C>G p.L718V EX18 44.6% missensePE424 SLX4 c.3761C>T p.S1254L EX12 1.4% missensePE364 EGFR c.2240_2257delTAAGAGAAGCAACATCTCp.L747_P753delinsSEX19 20.1% cds-indelPE448 ABCB1 c.3446C>A p.A1149E EX27 1.5% missensePE457 ACVR1B c.1342G>T p.G448W EX8 5.2% missensePE396 EGFR c.2573T>G p.L858R EX21 2.5% missensePE550 LRP1B c.7742C>G p.S2581C EX47 1.3% missensePE581 BRD4 c.3835G>T p.A1279S EX19 45.2% missensePE599 ALK c.3512T>C p.I1171T EX22 4.2% missensePE660 LRP1B c.11615A>G p.N3872S EX76 5.9% missensePE630 EGFR c.2303G>T p.S768I EX20 84.5% missensePE669 EGFR c.2153T>A p.L718Q EX18 4.3% missensePE689 ABCA13 c.10269C>G p.I3423M EX30 2.1% missensePE740 PIK3R2 c.1600G>A p.E534K EX13 1.0% missensePE883 EPHB1 c.2942C>T p.T981M EX16E 1.3% missensePE753 TGFBR2 c.338G>A p.W113* EX3 31.0% nonsensePE877 TP53 c.841G>C p.D281H EX8 13.7% missensePE885 SH2B3 c.31C>T p.P11S EX2 11.2% missensePE935 U2AF1 c.101C>T p.S34F EX2 24.1% missensePE270 EGFR c.2369C>T p.T790M EX20 8.0% missensePE223 PDK1 c.355C>T p.Q119* EX3 0.6% nonsensePE207 NOTCH3 c.6448C>T p.R2150C EX33 28.8% missensePE356 NF1 c.2062G>C p.E688Q EX18 21.9% missensePE394 NBN c.925G>A p.E309K EX8 8.9% missensePE366 TP53 c.747G>T p.R249S EX7 0.5% missensePE447 CDH1 c.1451C>T p.P484L EX10 2.8% missensePE500 KRTAP5-5 c.26G>T p.G9V EX1E 4.2% missensePE539 TP53 c.263C[5>4] p.S90Pfs*33 EX4 9.9% frameshift

PE585 EGFR c.2236_2250delGAATTAAGAGAAGCAp.E746_A750del EX19 31.9% cds-delPE511 POLD1 c.3244C>T p.R1082C EX27E 4.0% missensePE593 NOTCH4 c.813_815delAGAinsGGGp.D272G EX5 16.9% missensePE612 TP53 c.882_885delGCCTp.E294Dfs*50 EX8 47.8% frameshiftPE731 C3orf70 c.563A>G p.E188G EX2E 1.2% missensePE717 INHBA c.686G>A p.R229Q EX3E 2.8% missensePE686 ERBB2 c.3365C>G p.S1122C EX26 2.5% missensePE565 ROBO3 c.4087C>T p.R1363W EX27 1.2% missensePE770 SMAD2 c.830delT p.I277Kfs*6 EX8 5.5% frameshiftPE794 FANCF c.337C>T p.Q113* EX1E 27.9% nonsensePE651 TP53 c.378C>G p.Y126* EX5 6.8% nonsensePE850 TP53 c.582_583insG p.I195Dfs*14 EX6 12.6% frameshiftPE742 TP53 c.574C>T p.Q192* EX6 30.5% nonsensePE785 KDM6A c.3784C>T p.Q1262* EX26 53.5% nonsensePE876 MYCN c.834C>G p.D278E EX3E 1.2% missensePE656 TP53 c.614A>T p.Y205F EX6 4.4% missensePE891 CREBBP c.2234C[4>3] p.P746Qfs*2 EX12 3.3% frameshiftPE862 ALK c.398G>A p.R133H EX1 0.4% missensePE255 BCOR c.830C>T p.P277L EX4 3.5% missensePE252 ARID1A c.5901GT[2>1] p.C1968Yfs*30 EX20 83.9% frameshiftPE341 CASP1 c.98G>C p.R33T EX2 5.3% missensePE429 EGFR c.2573T>G p.L858R EX21 20.6% missensePE435 EGFR c.2235_2249delGGAATTAAGAGAAGCp.E746_A750del EX19 2.0% cds-delPE364 FAT2 c.10899G>A p.M3633I EX18 2.1% missensePE448 EGFR c.2369C>T p.T790M EX20 45.8% missensePE489 ABL2 c.1388C>G p.S463* EX8 2.6% nonsensePE457 GATA3 c.721G>T p.G241C EX3 2.2% missensePE576 CYP2D6 c.230C>T p.P77L EX2 1.0% missensePE588 CCNE1 c.391C>G p.Q131E EX6 2.1% missensePE553 TP53 c.848G>C p.R283P EX8 28.3% missensePE625 PALB2 c.1712A>T p.D571V EX5 7.4% missensePE599 MTOR c.424G>A p.A142T EX4 4.1% missensePE669 APC c.1072C>T p.Q358* EX10 1.2% nonsensePE763 FLT4 c.2629G>A p.A877T EX18 10.0% missensePE729 EGFR c.2573T>G p.L858R EX21 1.4% missensePE740 EGFR c.2300_2308dupCCAGCGTGGp.A767_V769dup EX20 2.3% cds-insPE793 PBRM1 c.2785G>T p.E929* EX19 12.0% nonsensePE563 NF1 c.5624C>G p.S1875* EX38 70.2% nonsensePE857 EGFR c.2235_2249delGGAATTAAGAGAAGCp.E746_A750del EX19 65.3% cds-delPE911 ANKRD30B c.3422C>T p.A1141V EX36E 1.6% missensePE885 TSC1 c.2152C>T p.R718W EX17 30.9% missensePE822 CSF1R c.1597C>T p.L533F EX11 15.9% missensePE735 CSF1R c.2543TCT[2>1] p.F849del EX19 5.1% cds-delPE816 PIK3CA c.323G>A p.R108H EX2 11.2% missensePE935 GIGYF1 c.527G>T p.R176L EX7 9.8% missensePE210 MPL c.502G>A p.E168K EX4 0.9% missensePE223 SMARCA4 c.3728G>T p.R1243L EX26 22.5% missensePE263 F8 c.4976_4977delCAinsACp.P1659H EX14 1.1% missensePE207 CBFB c.232C>T p.R78* EX3 3.3% nonsensePE279 RB1 c.763C>T p.R255* EX8 0.7% nonsensePE313 LRP1B c.11132-2A>G . IVS72 6.9% splice-3PE339 SLX4 c.4983G>C p.K1661N EX14 2.1% missensePE525 PIK3R1 c.1852C>T p.H618Y EX15 7.1% missensePE577 EGFR c.2573T>G p.L858R EX21 5.6% missense

PE594 ATM c.95G>T p.R32L EX3 28.8% missensePE561 AURKB c.479G>A p.G160E EX6 3.2% missensePE639 PIK3R1 c.2006A>T p.H669L EX16E 1.8% missensePE634 MYH9 c.1634A>T p.K545M EX14 3.9% missensePE718 GNAS c.1025G>A p.R342Q EX12 1.0% missensePE437 DICER1 c.3285C>G p.D1095E EX22 18.8% missensePE679 RB1 c.2359C>T p.R787* EX23 14.6% nonsensePE898 RBM10 c.1753_1754delACp.T585Cfs*18 EX14 22.0% frameshiftPE869 TP53 c.707A>G p.Y236C EX7 5.6% missensePE801 PDGFRA c.727G>T p.V243F EX5 11.1% missensePE223 CTNNB1 c.110C>T p.S37F EX3 1.2% missensePE222 SMARCA4 c.797C>T p.S266L EX5 1.9% missensePE251 PPM1D c.1714C>T p.R572* EX6 0.9% nonsensePE245 EGFR c.2235_2249delGGAATTAAGAGAAGCp.E746_A750del EX19 31.9% cds-delPE352 WT1 c.120G>A p.W40* EX1 54.5% nonsensePE356 MLL c.609G>T p.E203D EX3 30.3% missensePE460 SUFU c.703A>G p.I235V EX6 5.0% missensePE394 DIS3L2 c.1844C>G p.T615R EX15 19.9% missensePE448 KEAP1 c.1568G>A p.G523E EX5 1.4% missensePE500 LRP1B c.6548T>A p.L2183Q EX41 11.2% missensePE539 HNF1A c.1827C>A p.H609Q EX10E 1.4% missensePE562 FAS c.227A>G p.N76S EX3 4.0% missensePE585 EPHA5 c.2474T>A p.L825Q EX14 1.0% missensePE557 PIK3CG c.1613C>T p.P538L EX2 3.9% missensePE728 TGFBR2 c.1454G>T p.R485L EX6 4.7% missensePE736 CDK12 c.1373C>T p.P458L EX2 0.5% missensePE708 TGFBR1 c.1014delT p.N338Kfs*4 EX6 6.5% frameshiftPE495 TP53 c.637C>T p.R213* EX6 5.9% nonsensePE770 KDR c.3991G>A p.E1331K EX30E 7.5% missensePE830 HIST1H3F c.263C>T p.S88L EX1E 0.6% missensePE742 EGFR c.2573T>G p.L858R EX21 67.3% missensePE915 TP53 c.1015G>T p.E339* EX10 43.0% nonsensePE656 PTCH2 c.2696T>C p.I899T EX18 0.7% missensePE891 CCND1 c.815A>T p.E272V EX5E 2.4% missensePE935 PDGFRA c.727G>T p.V243F EX5 11.8% missensePE222 NFE2L2 c.92G>T p.G31V EX2 10.3% missensePE207 PCK1 c.442C>T p.P148S EX4 9.5% missensePE339 LRP1B c.359G>T p.C120F EX4 18.9% missensePE390 TP53 c.269C[2>1] p.W91Gfs*32 EX4 66.0% frameshiftPE370 PDK1 c.175A>G p.M59V EX1 6.0% missensePE543 MLL2 c.11680A>G p.M3894V EX39 6.5% missensePE474 EGFR c.2573T>G p.L858R EX21 5.2% missensePE497 MLL3 c.10253A>T p.E3418V EX43 9.5% missensePE518 EGFR c.2573T>G p.L858R EX21 8.9% missensePE594 TLX3 c.706G>A p.A236T EX3E 35.3% missensePE720 EGFR c.2239_2248delinsCp.L747_A750delinsPEX19 39.0% cds-indelPE762 NOTCH3 c.5410G>T p.E1804* EX30 41.7% nonsensePE761 NCOR1 c.1453G>T p.E485* EX14 15.5% nonsensePE615 EGFR c.2235_2249delGGAATTAAGAGAAGCp.E746_A750del EX19 0.90% cds-delPE640 ERCC2 c.2205_2216delCCTGTCCCTGCTinsACAp.L736_L739delinsHEX23E 9.8% cds-indelPE679 CSMD3 c.10973C>A p.P3658H EX71E 2.5% missensePE832 DPYD c.560C>T p.S187F EX6 10.6% missensePE788 ERCC5 c.3386C>T p.S1129L EX15E 1.7% missensePE743 TP53 c.743G>A p.R248Q EX7 51.6% missense

PE889 ABL1 c.1425T>A p.C475* EX9 2.2% nonsensePE857 EXT1 c.1258G>C p.E420Q EX4 11.7% missensePE790 FLCN c.1538+2_1538+3delTA. IVS13 5.5% splice-5PE360 EPHA3 c.1121A>G p.E374G EX5 0.5% missensePE658 STAG2 c.894-1G>A . IVS10 1.0% splice-3PE861 TAF1L c.4360C>T p.R1454C EX1E 1.7% missensePE655 ATM c.1009C>T p.R337C EX8 26.5% missensePES131 EGFR c.2236_2250delGAATTAAGAGAAGCAp.E746_A750del EX19 0.4% cds-delPE219 BRCA2 c.9098C>G p.T3033S EX23 5.0% missensePE336 FLT4 c.2830G>T p.D944Y EX20 0.7% missensePE314 DNMT3A c.855+1G>A . IVS7 1.2% splice-5PE215 EPHA7 c.2648T>C p.F883S EX15 16.4% missensePE403 TP53 c.592G>T p.E198* EX6 4.9% nonsensePE461 DRGX c.655C>T p.R219C EX6E 2.4% missensePE417 EPHB6 c.557C>T p.A186V EX7 12.2% missensePE505 PAK3 c.581G>T p.R194I EX9 5.4% missensePE639 FLT1 c.2656G>T p.G886C EX19 8.6% missensePE702 EGFR c.2369C>T p.T790M EX20 10.4% missensePE599 EPHB4 c.2492A>G p.N831S EX15 43.0% missensePE481 TP53 c.326T>C p.F109S EX4 6.6% missensePE671 CBLB c.1213G>T p.G405C EX10 36.5% missensePE566 IFNG c.388C>A p.R130S EX4E 6.4% missensePE814 EGFR c.2573T>G p.L858R EX21 1.5% missensePE839 LRP1B c.301T>A p.L101M EX3 1.3% missensePE884 PIK3R1 c.1681A>G p.K561E EX13 74.5% missensePE869 CHEK2 c.610_612delCTGinsATTp.L204I EX5 3.2% missensePE223 SDHA c.251G>A p.G84E EX3 2.8% missensePE303 BRAF c.1796C>G p.T599R EX15 8.4% missensePE207 ABL2 c.1993C[6>7] p.K667Qfs*15 EX12 18.3% frameshiftPE204 PIK3R1 c.1402G>A p.E468K EX11 13.8% missensePE453 EGFR c.2369C>T p.T790M EX20 3.3% missensePE503 TP53 c.856G>T p.E286* EX8 15.5% nonsensePE546 TOP2A c.2812C>G p.Q938E EX23 1.2% missensePES121 TP53 c.844C>T p.R282W EX8 61.6% missensePE214 ABL1 c.3202G>A p.V1068M EX11E 20.6% missensePE728 STK11 c.475C>T p.Q159* EX4 0.8% nonsensePE792 SMAD4 c.988G>C p.E330Q EX9 3.4% missensePE857 JUN c.334G>C p.E112Q EX1E 7.1% missensePE876 CREBBP c.6920C>T p.S2307F EX31E 1.7% missensePE869 RET c.1148G>T p.G383V EX6 7.1% missensePE858 DNMT3A c.918G>A p.W306* EX8 1.2% nonsensePE252 PIK3CB c.2570C>G p.S857C EX18 3.8% missensePE270 HCLS1 c.484_485delGGinsTTp.G162L EX7 14.3% missensePE334 EGFR c.2573T>G p.L858R EX21 35.4% missensePE264 LRP1B c.2298G>T p.L766F EX14 38.4% missensePE378 FAT2 c.5374A>C p.S1792R EX9 3.5% missensePE364 SMARCA4 c.2895C>G p.I965M EX20 31.6% missensePE477 APC c.4661A[6>7] p.T1556Nfs*3 EX16E 53.5% frameshiftPE418 ZFHX3 c.8084G>T p.R2695L EX9 4.7% missensePE517 MED12 c.4205G>A p.S1402N EX30 1.8% missensePE612 ROS1 c.4705C>T p.R1569W EX29 0.5% missensePE628 PCSK6 c.898G>A p.D300N EX7 7.8% missensePE660 RBM10 c.2398G>T p.E800* EX20 5.1% nonsensePE689 FANCM c.2227C>G p.P743A EX13 1.2% missense

PE682 FCGR3A c.717C>A p.D239E EX6E 14.8% missensePE787 MLL3 c.5224C>A p.Q1742K EX35 3.2% missensePE888 PIK3CA c.1974G>C p.L658F EX13 2.7% missensePE703 TP53 c.536A>T p.H179L EX5 65.4% missensePE852 PPM1D c.1434C>A p.C478* EX6E 0.5% nonsensePE886 XPC c.1040C>T p.S347L EX9 10.9% missensePE735 MLL c.3302G>A p.R1101Q EX4 2.2% missensePE900 LRP1B c.80G>A p.R27Q EX1 1.0% missensePE592 GLI3 c.2204G>C p.G735A EX14 4.3% missensePE933 TERT c.-58-u66C>T . . 6.4% promoterPE921 GRM3 c.637_644delGTGTCCACp.V213Sfs*6 EX3 0.8% frameshiftPE816 NCOR1 c.2060C>T p.S687L EX19 2.4% missensePES130 PDGFRB c.2614C>G p.P872A EX19 1.0% missensePE308 MLL3 c.1304G>A p.C435Y EX10 0.6% missensePE303 MSH6 c.1835C>G p.S612* EX4 4.5% nonsensePE301 STAG2 c.314C>T p.S105L EX6 8.8% missensePE207 CDK12 c.4376G[7>8] p.T1463Nfs*50 EX14 16.6% frameshiftPE370 JARID2 c.2999C[2>1] p.V1001Cfs*47 EX14 4.2% frameshiftPE493 RBM10 c.1421C>T p.A474V EX12 3.0% missensePE558 PIK3R1 c.1527A>G p.I509M EX12 1.7% missensePE442 SMARCA4 c.296G>C p.R99P EX3 43.2% missensePE731 EGFR c.2573T>G p.L858R EX21 71.2% missensePE694 NF1 c.5914C>T p.Q1972* EX39 27.2% nonsensePE857 DAXX c.39G>C p.E13D EX1 29.5% missensePE856 NOTCH1 c.4116C>G p.C1372W EX25 19.2% missensePE847 NCOR1 c.2618C>T p.P873L EX20 2.7% missensePE904 TP53 c.644G>T p.S215I EX6 7.1% missensePE922 APC c.6914G>C p.R2305T EX16E 19.1% missensePE252 LRP1B c.7534_7535delGCinsAAp.A2512N EX46 3.4% missensePE270 DNMT3A c.1198G>T p.E400* EX10 16.1% nonsensePE264 CREBBP c.4336C>T p.R1446C EX26 39.8% missensePE344 TP53 c.1024C>T p.R342* EX10 29.4% nonsensePE487 EPHA5 c.721G>A p.A241T EX3 26.2% missensePE442 ERBB4 c.1787G>T p.G596V EX15 41.5% missensePE517 EGFR c.2573T>G p.L858R EX21 5.2% missensePE579 KRAS c.35G>A p.G12D EX2 1.0% missensePE636 EPHA3 c.1234G>A p.V412I EX5 14.3% missensePE654 LRP1B c.2812C>A p.R938S EX18 4.5% missensePE628 EGFR c.1951G>A p.V651M EX17 55.1% missensePE705 RBM10 c.454A>T p.R152W EX4 1.0% missensePE689 MLL2 c.7364C>T p.S2455L EX31 2.5% missensePE680 ARID1A c.5965C>T p.R1989* EX20E 19.9% nonsensePE757 SMARCB1 c.91G>C p.E31Q EX1 21.7% missensePE787 EGFR c.2236_2250delGAATTAAGAGAAGCAp.E746_A750del EX19 2.7% cds-delPE840 EGFR c.2369C>T p.T790M EX20 0.3% missensePE592 TET2 c.558G>C p.E186D EX3 6.4% missensePES130 MTOR c.6073G>A p.E2025K EX44 0.8% missensePE212 FCGR2B c.778G>C p.E260Q EX6 0.8% missensePE260 FAT2 c.1882C>G p.L628V EX1 0.7% missensePE263 TP53 c.313G>C p.G105R EX4 30.5% missensePE207 EPHA5 c.533C>A p.A178D EX3 6.7% missensePE279 RB1 c.2548C>T p.Q850* EX25 1.4% nonsensePE606 IL7R c.965G>A p.G322D EX8 1.2% missensePE339 OMD c.1145G>C p.R382T EX3 0.7% missense

PE320 GRIN2A c.2747A>G p.D916G EX13E 1.2% missensePE434 PCK1 c.524G>A p.R175Q EX4 5.9% missensePE454 EGFR c.2369C>T p.T790M EX20 20.1% missensePE428 LRP4 c.1060G>C p.G354R EX10 6.2% missensePE569 TGFBR1 c.680AAG[2>1] p.E227[2>1] EX4 1.0% cds-delPE594 EPHB6 c.734C>T p.T245M EX7 4.6% missensePE639 RNASEL c.1137C>G p.I379M EX2 5.9% missensePE777 PIK3C2G c.4180G>A p.E1394K EX31 1.0% missensePE608 AR c.1954C>G p.P652A EX4 4.1% missensePE849 EGFR c.2369C>T p.T790M EX20 7.3% missensePE772 TP53 c.110C>A p.S37Y EX4 7.4% missensePE491 EPHA2 c.1682+1G>T . IVS8 1.0% splice-5PE898 BRAF c.1762C>T p.L588F EX15 28.1% missensePE360 DOT1L c.3346C>G p.L1116V EX24 1.3% missensePE912 NCOR1 c.2386_2403delATGGATGTAGATCAGCAGp.M796_Q801del EX20 2.3% cds-delPE894 EGFR c.2369C>T p.T790M EX20 10.6% missensePE869 ZFHX3 c.1512_1513delGGinsTTp.E504_D505delinsDYEX2 2.4% missensePE923 CBFB c.7C>T p.R3C EX1 3.5% missensePE693 ESR1 c.860G>A p.R287K EX5 8.8% missensePE270 SCUBE2 c.1870G>A p.A624T EX16 5.5% missensePE223 MLL4 c.3185C>A p.P1062Q EX8 1.4% missensePE207 PBRM1 c.3818A>G p.Q1273R EX25 27.3% missensePE356 ERBB2 c.929C>A p.S310Y EX8 18.8% missensePE388 TP53 c.844C>T p.R282W EX8 6.2% missensePE428 ARID1A c.2128C>T p.R710C EX5 10.3% missensePE500 KRAS c.35G>T p.G12V EX2 30.8% missensePE542 CSF1R c.1879_1881delAAGp.K627del EX14 3.2% cds-delPE593 FANCA c.4225C>T p.R1409W EX42 16.4% missensePE713 EGFR c.2239_2264delTTAAGAGAAGCAACATCTCCGAAAGCinsGCCAAp.L747_A755delinsANEX19 18.5% cds-indelPE728 CSF1R c.567C>G p.I189M EX4 3.6% missensePE767 RECQL4 c.166G>T p.G56C EX3 1.1% missensePE794 ZFP36L1 c.718C>G p.P240A EX3E 42.6% missensePE791 CDH23 c.3670A>G p.I1224V EX31 0.7% missensePE891 STAT3 c.1876_1878delAAGp.K626del EX20 3.2% cds-delPE220 RBM10 c.1160C>T p.P387L EX10 41.3% missensePE325 FAT2 c.461G>T p.R154I EX1 16.8% missensePE336 RBM10 c.966dupA p.L323Tfs*43 EX9 20.4% frameshiftPE208 FAT1 c.8319G>A p.W2773* EX10 0.5% nonsensePE403 NFE2L2 c.88C>T p.L30F EX2 3.7% missensePE429 ATM c.4099G>A p.D1367N EX27 10.9% missensePE483 PIK3C2G c.4180G>A p.E1394K EX31 1.3% missensePE472 ARID1A c.595C>T p.Q199* EX1 7.1% nonsensePE461 KDR c.1298A>T p.D433V EX10 1.1% missensePE417 FAT1 c.11914G>C p.G3972R EX22 10.0% missensePE473 APC c.2404G>C p.D802H EX16E 13.0% missensePE524 ELAC2 c.674C>T p.P225L EX7 10.0% missensePE531 FBXW7 c.336T>G p.D112E EX2 2.3% missensePE528 PIK3CG c.530G>A p.R177H EX2 1.0% missensePE594 NOTCH3 c.625G>A p.G209R EX4 14.5% missensePE512 EGFR c.2573T>G p.L858R EX21 96.8% missensePE614 MED12 c.1862G>A p.R621Q EX13 1.2% missensePE690 NTRK1 c.218T>C p.I73T EX2 1.8% missensePE480 PTEN c.274G>T p.D92Y EX5 1.2% missensePE222 KIT c.395C>T p.T132M EX3 16.1% missense

PE774 TTF1 c.2176G>A p.E726K EX7 4.0% missensePE857 ARHGAP35 c.3963G>T p.M1321I EX4 29.1% missensePE882 EGFR c.2369C>T p.T790M EX20 0.2% missensePE869 KEAP1 c.724G>A p.E242K EX3 6.9% missensePE828 SERPINB4 c.483C>A p.N161K EX6 38.6% missensePE299 PBRM1 c.242_246delAACCAp.Q81Rfs*4 EX4 18.9% frameshiftPE315 EGFR c.2369C>T p.T790M EX20 7.2% missensePE207 JAK2 c.3338G>A p.R1113H EX25 10.3% missensePE317 EPHA5 c.314T>G p.M105R EX3 22.5% missensePE328 RARA c.1021G>A p.D341N EX8 1.0% missensePE390 EGFR c.2573T>G p.L858R EX21 36.3% missensePE374 EGFR c.2573T>G p.L858R EX21 7.4% missensePE465 MLL3 c.377A>G p.E126G EX3 0.8% missensePE485 PCK1 c.1755G>T p.W585C EX10E 16.4% missensePE543 CASP8 c.1456C>T p.Q486* EX8 8.2% nonsensePE594 EPHA5 c.797C>A p.S266Y EX3 19.5% missensePE712 NUP210 c.3442G>C p.E1148Q EX25 16.8% missensePE696 INPP4B c.2566G>C p.E856Q EX25 0.6% missensePE784 TP53 c.994-1G>A . IVS9 38.4% splice-3PE776 ASXL1 c.3187C>T p.Q1063* EX13E 0.5% nonsensePE355 POLE c.6716C>T p.A2239V EX48 1.9% missensePE814 RET c.335G>A p.R112H EX2 0.4% missensePE812 BLM c.2433C>G p.Y811* EX12 40.9% nonsensePE914 PTCH2 c.2263G>A p.A755T EX15 11.6% missensePE216 EPHA5 c.2497T>G p.Y833D EX14 0.9% missensePE303 ARID1A c.1338_1339delTTp.Q449Afs*173 EX2 3.3% frameshiftPE207 CAMTA1 c.2429C>T p.A810V EX9 10.9% missensePE328 EGFR c.2573T>G p.L858R EX21 67.1% missensePE330 TP53 c.830G>T p.C277F EX8 12.7% missensePE204 BRD4 c.2419G>T p.V807F EX13 20.3% missensePE390 PDK1 c.513_523delTGTCACCAGCCp.V172Efs*17 EX4 42.7% frameshiftPE413 TP53 c.736A>G p.M246V EX7 65.0% missensePE367 EGFR c.2239_2240delTTinsCCp.L747P EX19 9.6% missensePE459 EPHB6 c.1996G>T p.D666Y EX14 2.5% missensePE485 FGFR1 c.878T>A p.I293N EX7 7.8% missensePE387 RBM10 c.1410delT p.A471Pfs*79 EX12 20.1% frameshiftPES130 TP53 c.842A>G p.D281G EX8 30.2% missensePE442 RBM10 c.2759G>T p.G920V EX23 93.0% missensePE594 PMS1 c.2755C>T p.R919C EX13E 14.6% missensePE561 EGFR c.2369C>T p.T790M EX20 45.0% missensePE629 NBN c.1378C>T p.Q460* EX10 0.5% nonsensePE601 EGFR c.2573T>G p.L858R EX21 1.3% missensePE784 EGFR c.2573T>G p.L858R EX21 46.8% missensePE640 EPHA3 c.2060G>C p.G687A EX11 19.1% missensePE716 EGFR c.2235_2249delGGAATTAAGAGAAGCp.E746_A750del EX19 11.1% cds-delPE808 ERCC4 c.88G>A p.D30N EX1 6.2% missensePE807 PTGS1 c.1679C>T p.T560M EX11E 32.4% missensePE663 TP53 c.470T>C p.V157A EX5 9.5% missensePE906 POM121L12c.568G>A p.A190T EX1E 10.5% missensePE206 TET2 c.2428C>T p.Q810* EX3 0.8% nonsensePE369 EGFR c.2235_2249delGGAATTAAGAGAAGCp.E746_A750del EX19 9.6% cds-delPE205 PIK3CA c.1624G>A p.E542K EX10 0.7% missensePE424 ARID1B c.220G>A p.E74K EX1 1.0% missensePE466 EGFR c.2582T>A p.L861Q EX21 0.9% missense

PE548 TP53 c.395A>G p.K132R EX5 69.9% missensePES121 TYR c.1088A>T p.H363L EX3 0.7% missensePE638 SLX4 c.2623G>A p.E875K EX12 2.8% missensePE587 TP53 c.380C>T p.S127F EX5 11.0% missensePE687 IRS2 c.2901C>G p.S967R EX1 1.7% missensePE619 IGF1R c.2449G>A p.A817T EX11 0.8% missensePE748 FAT1 c.4184-1G>T . IVS6 12.0% splice-3PE840 SMARCE1 c.730G>C p.E244Q EX9 5.7% missensePE839 TP53 c.686_687delGT p.C229Yfs*10 EX7 17.1% frameshiftPE937 TP63 c.1200delG p.L401Yfs*5 EX9 0.7% frameshiftPE930 SMAD4 c.1081C>G p.R361G EX9 5.5% missensePE223 TP53 c.106C>T p.P36S EX4 0.9% missensePE283 EGFR c.2573T>G p.L858R EX21 7.7% missensePE309 TP53 c.818G>T p.R273L EX8 28.0% missensePE207 EPHA2 c.1374C[6>5] p.P460Rfs*33 EX6 18.7% frameshiftPE348 TP53 c.614A>G p.Y205C EX6 9.2% missensePE356 FAM123B c.673A>G p.R225G EX2 45.0% missensePE503 EGFR c.2235_2249delGGAATTAAGAGAAGCp.E746_A750del EX19 7.7% cds-delPE428 TNFAIP3 c.2306A>G p.H769R EX9E 1.6% missensePE546 CBL c.1100A>C p.Q367P EX8 1.7% missensePE533 C11orf30 c.565G>A p.V189I EX6 1.2% missensePE593 ABL1 c.2951C>T p.T984M EX11E 4.8% missensePE664 RICTOR c.2614C>A p.Q872K EX27 2.2% missensePE535 TP53 c.527G>T p.C176F EX5 14.7% missensePE730 EGFR c.2369C>T p.T790M EX20 7.2% missensePE760 RET c.2672C>T p.S891L EX15 3.4% missensePE679 NOTCH2 c.4900C>G p.Q1634E EX27 1.0% missensePE564 DICER1 c.1343T>G p.V448G EX9 56.1% missensePE786 EPHA3 c.1133C>A p.P378Q EX5 3.5% missensePE840 PIK3CA c.1633G>A p.E545K EX10 1.5% missensePE800 KRAS c.35G>A p.G12D EX2 22.2% missensePE876 PTPRD c.3181G>T p.E1061* EX30 1.8% nonsensePE854 EGFR c.2235_2249delGGAATTAAGAGAAGCp.E746_A750del EX19 5.6% cds-delPE291 TP53 c.814G>A p.V272M EX8 3.2% missensePE273 ARAF c.641C>T p.S214F EX7 12.9% missensePE392 KRAS c.35G>T p.G12V EX2 9.6% missensePE205 PTPRD c.2616G>T p.E872D EX28 0.7% missensePE638 BRCA1 c.4606G>A p.E1536K EX14 2.2% missensePE587 RARA c.574C>T p.R192C EX5 7.7% missensePE650 CBLB c.1235G>A p.R412H EX10 38.7% missensePE748 CSMD3 c.11039_11040delCCinsAAp.P3680Q EX71E 15.2% missensePE220 TBX3 c.1090A>G p.N364D EX6 8.7% missensePE222 LRP1B c.12820A>T p.S4274C EX84 25.2% missensePE336 TERT c.-58-u66C>T . . 27.9% promoterPE324 CDKN2A c.322G>T p.D108Y EX2 33.2% missensePE207 GNAS c.307A>T p.I103F EX4 1.8% missensePE338 ATM c.8203T>C p.C2735R EX56 2.4% missensePE363 BBS9 c.535C>T p.L179F EX6 11.3% missensePE370 MET c.3028+2T>A . IVS14 0.9% splice-5PE425 EGFR c.2573T>G p.L858R EX21 3.4% missensePE429 HGF c.1732G>A p.D578N EX15 25.6% missensePE465 PER1 c.2678G>T p.G893V EX19 81.9% missensePE417 LRP1B c.9176G>T p.W3059L EX58 37.4% missensePE555 ATR c.7018G>A p.E2340K EX41 5.1% missense

PE524 DNMT3A c.2591T>A p.M864K EX22 1.2% missensePE531 ALK c.3806G>C p.G1269A EX25 16.9% missensePE594 TP53 c.614A>T p.Y205F EX6 5.8% missensePE509 GNAQ c.190G>T p.G64W EX2 1.3% missensePE572 LRP1B c.2644+1G>A . IVS16 36.4% splice-5PE690 JAK1 c.2570C>G p.S857* EX19 1.5% nonsensePE644 MLH1 c.646A>G p.I216V EX8 18.0% missensePE749 HOXA3 c.380C>T p.P127L EX3 14.4% missensePE798 LEF1 c.984_988delTATCAp.I329Pfs*13 EX8 1.6% frameshiftPE828 MLL c.10559C>G p.S3520C EX27 38.2% missensePE219 MLL2 c.10328G>T p.S3443I EX35 1.4% missensePE223 ALK c.3305C>A p.T1102N EX20 0.4% missensePE336 ARID1A c.4786G>C p.E1596Q EX18 7.2% missensePE215 PIK3CA c.331A>G p.K111E EX2 30.2% missensePE322 LRP1B c.1692C>A p.H564Q EX11 38.7% missensePE436 FGFR3 c.1489G>C p.D497H EX11 4.0% missensePE429 POLE c.4396G>C p.E1466Q EX34 27.6% missensePE448 C3orf70 c.628G>A p.G210R EX2E 1.9% missensePE417 HGF c.1120G>T p.V374F EX9 65.3% missensePE531 GNAQ c.175A>C p.M59L EX2 2.4% missensePE702 FAT2 c.4390G>C p.G1464R EX7 13.8% missensePE644 FBXW7 c.800AT[2>1] p.M268Dfs*18 EX5 16.9% frameshiftPE222 KRAS c.35G>T p.G12V EX2 56.2% missensePE697 PDGFRA c.572C>T p.A191V EX4 7.8% missensePE858 ERBB4 c.1979G>T p.G660V EX17 14.4% missensePE801 PTPRD c.104G>T p.G35V EX13 17.2% missensePE303 HOXA13 c.1113G>C p.Q371H EX2 5.5% missensePE301 RBM10 c.1889-2A>T . IVS15 16.3% splice-3PE207 BLM c.911C>A p.S304Y EX4 17.9% missensePE204 NBN c.38G>A p.G13E EX2 2.2% missensePE416 MLL3 c.12593_12611delGTTGTCATTGTAAAGTGGTp.C4198Lfs*14 EX50 1.2% frameshiftPE413 NOTCH2 c.629C>T p.P210L EX4 1.2% missensePES121 MTOR c.4180G>T p.A1394S EX28 0.6% missensePE493 TP53 c.838_846delAGAGACCGGp.D281_R283del EX8 4.3% cds-delPE580 FH c.986A>C p.N329T EX7 7.5% missensePE586 MLL c.7405G>C p.D2469H EX27 30.4% missensePE354 HSP90AA1 c.2428A>T p.I810F EX11 26.2% missensePE728 BCOR c.3551A>G p.E1184G EX8 8.0% missensePE732 EGFR c.2155G>T p.G719C EX18 12.7% missensePE694 CBL c.32C[2>1] p.G13Afs*13 EX1 28.2% frameshiftPE776 FOXP1 c.1653delT p.N552Tfs*47 EX15 0.5% frameshiftPE840 TP53 c.818G>A p.R273H EX8 82.2% missensePE807 RAD54L c.1117G>T p.D373Y EX11 29.8% missensePE847 EPHB1 c.2684C>T p.T895I EX14 1.2% missensePE223 KRAS c.34G>A p.G12S EX2 29.0% missensePE223 GRIN2A c.824G>A p.G275E EX3 20.3% missensePE207 MED12 c.4006G[4>3] p.E1337Kfs*8 EX28 3.1% frameshiftPE280 ERBB4 c.95C>G p.T32R EX2 1.0% missensePE313 XPC c.2734G>C p.E912Q EX16 4.3% missensePE339 CBFB c.232C>T p.R78* EX3 1.3% nonsensePE332 BARD1 c.1518_1519delTGinsCAp.V507M EX6 10.9% missensePE408 EGFR c.2236_2250delGAATTAAGAGAAGCAp.E746_A750del EX19 3.4% cds-delPE412 DDR2 c.1043G>A p.C348Y EX9 4.1% missensePE379 MED12 c.4211_4213delCAAp.A1404_S1405delinsGEX30 2.7% cds-indel

PE429 KIT c.1775-1G>T . IVS11 11.5% splice-3PE483 EGFR c.2573T>G p.L858R EX21 17.2% missensePE428 FANCM c.4478G>A p.R1493K EX17 8.5% missensePE525 EGFR c.2573T>G p.L858R EX21 80.1% missensePE584 FLCN c.247G>A p.E83K EX4 1.4% missensePE577 TP53 c.204G[2>1] p.A69Lfs*54 EX4 3.9% frameshiftPE594 H3F3C c.241G>A p.D81N EX1E 29.0% missensePE509 TRIO c.1922A>G p.D641G EX11 2.1% missensePE679 FANCM c.924G>T p.L308F EX5 10.4% missensePE684 KDM5A c.16C>T p.P6S EX1 72.0% missensePE898 AR c.1001G>A p.G334E EX1 9.2% missensePE875 MLH3 c.3528G>C p.L1176F EX5 4.3% missensePE631 EGFR c.2235_2249delGGAATTAAGAGAAGCp.E746_A750del EX19 26.3% cds-delPE828 FAT2 c.12649C>T p.P4217S EX23E 1.0% missensePE693 NTRK2 c.2375G>A p.R792H EX21E 11.5% missensePE342 EPHA3 c.2116T>C p.S706P EX12 11.4% missensePE346 HDAC1 c.463G>T p.D155Y EX5 0.7% missensePE404 NSD1 c.5400delA p.S1801Lfs*20 EX16 4.4% frameshiftPE364 BTK c.866G>T p.S289I EX10 20.1% missensePE477 TP53 c.626_629delGAAAp.R209Tfs*37 EX6 45.0% frameshiftPE406 DNMT3A c.2204A>G p.Y735C EX19 0.9% missensePE522 EGFR c.2573T>G p.L858R EX21 4.4% missensePE411 TP53 c.447_455dupCACACCCCCp.T150_P152dup EX5 22.4% cds-insPE689 SMARCE1 c.547G>A p.D183N EX8 1.1% missensePE648 MET c.3028+1G>A . IVS14 10.0% splice-5PE765 KRAS c.33TGG[2>3] p.G12[2>3] EX2 5.3% cds-insPE657 ARID1A c.476C>T p.S159F EX1 4.5% missensePE725 TP53 c.661G>T p.E221* EX6 55.4% nonsensePE866 GRIN2A c.4357G>A p.V1453M EX13E 1.3% missensePE886 KEAP1 c.899A>C p.Y300S EX3 1.6% missensePE863 RAD52 c.1040C>T p.S347F EX11 15.3% missensePE891 EPAS1 c.440T>G p.L147R EX4 2.4% missensePE896 FAT2 c.6363T>A p.Y2121* EX9 0.6% nonsensePE934 PIK3CG c.2634G>T p.M878I EX8 9.9% missensePE592 PSMB5 c.148G>A p.E50K EX1 4.6% missensePE816 FAM123B c.1640C>G p.S547C EX2E 1.2% missensePE918 EPHB6 c.757G>T p.A253S EX7 2.3% missensePE223 EGFR c.2240_2254delTAAGAGAAGCAACATp.L747_T751del EX19 0.9% cds-delPE269 TGFBR1 c.214A>G p.I72V EX2 1.1% missensePE274 FAT1 c.8299G>A p.D2767N EX10 6.9% missensePE252 LRP4 c.1994G>A p.S665N EX15 39.3% missensePE315 BRD4 c.2489T>A p.L830Q EX13 0.6% missensePE341 RNF43 c.788G>A p.G263E EX7 1.6% missensePE287 FOXL2 c.514G>A p.A172T EX1 14.7% missensePE331 ALK c.775C>T p.R259C EX2 1.7% missensePE395 TGFBR1 c.1126A>G p.K376E EX6 2.7% missensePE368 CDK8 c.80T>G p.V27G EX1 2.8% missensePE388 ARID2 c.1995delA p.Q666Kfs*35 EX15 19.3% frameshiftPE383 NF1 c.1666G>T p.D556Y EX15 1.3% missensePE489 TGFBR2 c.848T>A p.V283D EX5 3.1% missensePE457 TP53 c.920-2A>G . IVS8 7.3% splice-3PE550 C1S c.275A>G p.H92R EX4 3.6% missensePE545 ERBB2 c.929C>T p.S310F EX8 5.3% missensePE528 EGFR c.2573T>G p.L858R EX21 35.5% missense

PE578 INPP4B c.749G>A p.R250Q EX12 42.8% missensePE511 C8orf34 c.1095G>A p.M365I EX7 1.2% missensePE604 EGFR c.2369C>T p.T790M EX20 0.3% missensePE552 MLL3 c.5078A>C p.Y1693S EX34 15.8% missensePE551 STK11 c.940C[2>1] p.P314Lfs*22 EX8 37.7% frameshiftPE625 RBM10 c.2611A>T p.K871* EX21 7.8% nonsensePE660 EPHA3 c.797G>C p.R266T EX3 4.9% missensePE669 TSC1 c.379G>A p.V127I EX6 0.7% missensePE689 CSMD1 c.8638G>A p.V2880I EX56 1.3% missensePE793 IRS2 c.3784C>T p.P1262S EX1 8.8% missensePE835 BCOR c.4514G>T p.C1505F EX11 0.5% missensePE816 TP53 c.1044G[2>1] p.E349Nfs*21 EX10 3.7% frameshiftPE878 PIK3CA c.2176G>A p.E726K EX14 1.0% missensePE883 MET c.3028+1_3028+10delGTATATTTCA. IVS14 1.3% splice-5PE892 MYCN c.1082G>T p.S361I EX3E 2.9% missensePE870 POLE c.5179_5193delGTGGAGCTGGACCTTinsCp.V1727Pfs*53 EX39 2.5% frameshiftPE216 TP53 c.833C>G p.P278R EX8 2.6% missensePE303 MTOR c.1214C>G p.S405C EX8 3.6% missensePE207 BRCA1 c.1313A>G p.E438G EX10 10.9% missensePE328 TP53 c.701A>G p.Y234C EX7 89.8% missensePE330 EGFR c.2573T>G p.L858R EX21 14.1% missensePE390 PIK3C2B c.74C>T p.A25V EX3 12.1% missensePE459 EGFR c.2573T>G p.L858R EX21 7.3% missensePE485 OGDH c.830G>T p.R277L EX7 26.4% missensePE387 TP53 c.770T>G p.L257R EX7 27.5% missensePE536 EGFR c.2573T>G p.L858R EX21 1.4% missensePES130 EGFR c.2236_2250delGAATTAAGAGAAGCAp.E746_A750del EX19 21.6% cds-delPE594 LRP1B c.7688G>A p.R2563H EX47 18.2% missensePE642 PIK3R2 c.2017A>G p.T673A EX16E 10.3% missensePE696 LRP1B c.3420G>T p.K1140N EX22 0.5% missensePE716 FLT4 c.3538G>C p.E1180Q EX27 12.0% missensePE812 PTPRD c.5435G>T p.G1812V EX44 3.3% missensePE808 TSC1 c.1895A>T p.K632I EX15 6.7% missensePE878 JAK3 c.3205G>A p.E1069K EX23 1.0% missensePE825 KRAS c.35G>T p.G12V EX2 4.3% missensePE807 SYK c.463G>C p.E155Q EX3 1.4% missensePE663 SETD2 c.6014dupT p.S2006Kfs*2 EX12 16.1% frameshiftPE901 EGFR c.2573T>G p.L858R EX21 9.5% missensePE869 KRAS c.34G>T p.G12C EX2 17.2% missensePE906 TBL1XR1 c.382G>T p.E128* EX5 32.1% nonsensePE922 NSD1 c.437T>G p.I146S EX2 26.0% missensePE220 BRCA2 c.1857G>T p.Q619H EX10 23.9% missensePE222 TP53 c.533A>C p.H178P EX5 0.8% missensePE222 FGFR4 c.4C>T p.R2W EX2 9.5% missensePE336 NFE2L2 c.230A>T p.D77V EX2 24.6% missensePE324 LRP1B c.1170C>A p.Y390* EX8 25.0% nonsensePE207 COL5A2 c.2854C>T p.R952C EX42 1.3% missensePE363 EGFR c.2303_2311dupGCGTGGACAp.S768_D770dup EX20 30.7% cds-insPE370 TP53 c.800G>C p.R267P EX8 1.0% missensePE429 MLL3 c.2312C>T p.S771L EX14 22.7% missensePE461 MTOR c.2741C>G p.A914G EX18 8.2% missensePE417 PMS1 c.2174G>T p.C725F EX10 89.3% missensePE555 PIK3CA c.1624G>A p.E542K EX10 2.1% missensePE571 ARID1B c.124_126delGCGinsTCCp.A42S EX1 1.2% missense

PE531 CUL3 c.944A>T p.E315V EX7 2.0% missensePE594 NCOR1 c.5656G>A p.A1886T EX37 20.3% missensePE509 SF1 c.541G>A p.G181R EX6 35.0% missensePE572 CUL3 c.1801A>G p.I601V EX13 24.0% missensePE629 TP53 c.839G>T p.R280I EX8 80.2% missensePE690 JAK1 c.128C>T p.S43L EX3 2.1% missensePE783 MLL2 c.8711C>T p.P2904L EX34 33.2% missensePE679 TP53 c.991C>T p.Q331* EX9 10.6% nonsensePE805 ANKRD11 c.1106A>G p.K369R EX9 0.5% missensePE749 CSMD3 c.11039_11040delCCinsAAp.P3680Q EX71E 9.2% missensePE774 ERBB4 c.3416G>A p.R1139Q EX27 4.8% missensePE857 NTRK3 c.1432G>C p.A478P EX14 28.3% missensePE875 TAF1 c.3736C>T p.R1246W EX24 1.4% missensePE828 RB1 c.1672_1695+7delATGGAATCCCTTGCATGGCTCTCAGTAAGTA. EX17-IVS1710.9% spanPE801 CARD11 c.3080G>A p.R1027Q EX23 4.4% missensePE693 TP53 c.398T>C p.M133T EX5 35.0% missensePE296 OMD c.1171G>A p.E391K EX3 2.0% missensePE400 FANCD2 c.1102A>G p.I368V EX14 2.2% missensePE383 NBN c.1873G>A p.E625K EX12 29.4% missensePE457 ERBB4 c.437G>C p.G146A EX4 3.6% missensePE523 LRP1B c.9066T>A p.H3022Q EX57 10.1% missensePE547 EGFR c.2573T>G p.L858R EX21 9.1% missensePE574 TP53 c.584T>C p.I195T EX6 5.2% missensePE515 EGFR c.2240_2257delTAAGAGAAGCAACATCTCp.L747_P753delinsSEX19 0.4% cds-indelPE588 MLL3 c.9143_9144delTAp.L3048Rfs*38 EX38 2.4% frameshiftPE440 MLL2 c.6147G>A p.W2049* EX29 13.4% nonsensePE698 MAP3K1 c.2848G>C p.E950Q EX14 2.1% missensePE799 MLL3 c.1012+1G>T . IVS7 5.5% splice-5PE678 MLL3 c.10154T>A p.F3385Y EX43 9.3% missensePE748 TP53 c.461G>T p.G154V EX5 18.9% missensePE877 APC c.8204G>A p.G2735E EX16E 3.4% missensePE902 EGFR c.2369C>T p.T790M EX20 1.5% missensePE826 RB1 c.1422-1G>C . IVS15 93.6% splice-3PE821 EGFR c.2236_2250delGAATTAAGAGAAGCAp.E746_A750del EX19 0.5% cds-delPE632 FGF10 c.202C>T p.R68W EX1 1.7% missensePE931 EGFR c.2237_2251delAATTAAGAGAAGCAAp.E746_T751delinsAEX19 5.9% cds-indelPE816 ARID1A c.1907C>G p.P636R EX4 1.5% missensePE252 EPHB6 c.1204C>G p.L402V EX9 6.8% missensePE264 FAT2 c.11171T>C p.M3724T EX18 3.4% missensePE335 SMAD4 c.310_311delCT p.L104Sfs*4 EX3 5.2% frameshiftPE364 CIC c.935C>T p.S312F EX7 15.1% missensePE477 EPHB6 c.1043G>T p.C348F EX8 29.3% missensePE418 XRCC1 c.1691G>A p.R564Q EX15 0.6% missensePE411 BRCA2 c.7795G>A p.E2599K EX16 27.4% missensePE654 FAT1 c.331G>A p.E111K EX2 28.4% missensePE599 TP53 c.991C>T p.Q331* EX9 3.5% nonsensePE689 CREBBP c.2311C>T p.Q771* EX13 1.7% nonsensePE722 MSH2 c.76A>G p.M26V EX1 25.1% missensePE787 MAF c.14T>A p.L5Q EX1E 1.5% missensePE840 TP53 c.711G>A p.M237I EX7 1.1% missensePE888 PDILT c.1578G>T p.M526I EX12E 2.8% missensePE878 FAT2 c.859G>C p.E287Q EX1 1.1% missensePE703 SMARCA4 c.2932C>T p.R978* EX20 71.9% nonsensePE886 WRN c.2646G>T p.E882D EX22 4.0% missense

PE735 IRS2 c.1781C>T p.S594L EX1 2.0% missensePE863 VHL c.34G>C p.E12Q EX1 17.5% missensePE896 SPTA1 c.6602G>T p.R2201I EX48 0.7% missensePE907 FAT2 c.5608C>T p.P1870S EX9 7.8% missensePE920 MET c.3028delG p.D1010Ifs*19 EX14 13.2% frameshiftPE918 ELF3 c.912G>C p.K304N EX8 9.9% missensePE270 BRCA2 c.9932C>A p.P3311H EX27 21.4% missensePE351 TP53 c.535C>G p.H179D EX5 17.8% missensePE378 HDAC1 c.547G>A p.V183M EX6 7.3% missensePE344 TERT c.-58-u66C>T . . 21.6% promoterPE429 RBM10 c.2733-4_2741delGCAGAGACTTCGA. IVS22-EX231.4% spanPE424 RNF43 c.1820C>T p.S607L EX9 1.6% missensePE364 BRCA2 c.7366C>T p.Q2456* EX14 1.3% nonsensePE463 TFE3 c.1564G>C p.E522Q EX10E 22.1% missensePE503 EGFR c.2235_2249delGGAATTAAGAGAAGCp.E746_A750del EX19 3.2% cds-delPE582 FANCM c.5366G>A p.S1789N EX21 6.6% missensePE630 AXIN2 c.953G>A p.S318N EX3 50.3% missensePE733 FANCM c.5931A>C p.L1977F EX22 12.3% missensePE705 EGFR c.2504A>T p.H835L EX21 51.7% missensePE689 MLL c.10957C>G p.L3653V EX30 2.3% missensePE479 EGFR c.2573T>G p.L858R EX21 24.4% missensePE478 NF2 c.847T>C p.F283L EX9 2.6% missensePE787 RIT1 c.551G>T p.R184M EX6E 0.5% missensePE840 CTNNB1 c.95A>T p.D32V EX3 2.3% missensePE768 CCND1 c.340_342delAAG p.K114del EX2 1.6% cds-delPE892 TP53 c.874A[3>2] p.E294Sfs*51 EX8 8.7% frameshiftPE206 TP53 c.991C>T p.Q331* EX9 1.7% nonsensePE247 DNMT3A c.2478+1G>A . IVS21 1.3% splice-5PE322 RB1 c.2497_2503delGTATCAAinsACp.V833Tfs*3 EX24 27.6% frameshiftPE464 CTNNB1 c.110C>G p.S37C EX3 5.6% missensePE690 CBL c.1227+1G>T . IVS8 1.1% splice-5PE748 NFE2L2 c.235G>A p.E79K EX2 7.9% missensePE816 STK11 c.454C>T p.Q152* EX3 1.7% nonsensePE872 ROS1 c.1193C>G p.S398* EX11 3.0% nonsensePE801 TP53 c.473G>T p.R158L EX5 26.9% missensePE937 SF3B1 c.2221A>G p.K741E EX15 6.2% missensePE219 EGFR c.2573T>G p.L858R EX21 11.3% missensePE336 EGFR c.2156G>C p.G719A EX18 19.9% missensePE215 FCGR3A c.215C>T p.S72L EX4 1.3% missensePE322 LRP1B c.6653C>A p.P2218Q EX41 39.1% missensePE429 MLL2 c.8892delC p.N2965Tfs*39 EX34 26.5% frameshiftPE461 FAT2 c.3094T>A p.F1032I EX1 1.5% missensePE428 FRMPD4 c.3292G>A p.E1098K EX16 12.4% missensePE417 ARID1B c.1210C>T p.R404C EX1 57.0% missensePE473 EGFR c.2369C>T p.T790M EX20 1.0% missensePE505 ERBB3 c.1718G>T p.C573F EX15 7.8% missensePE639 MLL2 c.7790G>A p.S2597N EX31 1.4% missensePE702 FAT2 c.5068G>T p.V1690F EX9 19.1% missensePE697 EPHB1 c.1769G>A p.G590E EX10 34.9% missensePE749 ABCC11 c.3712C>G p.P1238A EX27 8.2% missensePE774 KCNJ5 c.473C>G p.T158R EX2 1.3% missensePE874 EGFR c.2235_2249delGGAATTAAGAGAAGCp.E746_A750del EX19 2.6% cds-delPE858 LRP1B c.11079T>A p.C3693* EX72 3.9% nonsensePE884 NOTCH2 c.7132G>A p.A2378T EX34E 21.7% missense

PE882 TP53 c.756delC p.T253Pfs*92 EX7 3.4% frameshiftPE828 RBM10 c.655G>A p.V219M EX5 5.6% missensePE209 RB1 c.1728A[3>2] p.K577Rfs*34 EX18 0.9% frameshiftPE303 TP53 c.725_736delGCATGGGCGGCAp.C242_M246delinsLEX7 2.0% cds-indelPE315 FANCM c.929C>T p.S310L EX5 10.0% missensePE207 LRP1B c.148T>G p.W50G EX2 10.5% missensePE317 CCND2 c.203A>C p.E68A EX2 45.3% missensePE328 TCF7L2 c.1391+2T>G . IVS13 14.2% splice-5PE367 TP53 c.528C[5>4] p.H178Tfs*69 EX5 5.8% frameshiftPE459 FAT3 c.12652G>A p.G4218S EX23 7.9% missensePE485 SMARCA4 c.4802G[2>1] p.K1602Rfs*8 EX34 14.0% frameshiftPE448 TP73 c.691A>T p.T231S EX6 62.1% missensePE468 EGFR c.2390G>C p.C797S EX20 15.7% missensePE526 ALK c.1826T>A p.L609Q EX10 30.0% missensePES130 TP53 c.842A>G p.D281G EX8 19.7% missensePE594 EPHB1 c.2293C>G p.L765V EX12 21.0% missensePE674 TP53 c.722C>A p.S241Y EX7 86.4% missensePE762 EGFR c.2573T>G p.L858R EX21 43.1% missensePE640 EGFR c.2369C>T p.T790M EX20 15.6% missensePE716 SMAD4 c.687G[6>5] p.G231Afs*10 EX6 18.9% frameshiftPE744 FAT2 c.830T>A p.V277D EX1 2.3% missensePE840 HSP90AB1 c.2036G>A p.R679H EX11 0.5% missensePE863 NOTCH3 c.5410G>T p.E1804* EX30 65.1% nonsensePE807 NCOR1 c.2382G>C p.E794D EX20 14.7% missensePE653 EGFR c.2369C>T p.T790M EX20 13.8% missensePE901 GRIN2A c.124C>A p.H42N EX2 5.0% missensePE869 GLI1 c.194-1G>T . IVS3 10.6% splice-3PE908 TP53 c.743G>A p.R248Q EX7 1.2% missensePE250 CDK12 c.2238C>G p.D746E EX4 6.2% missensePE254 TP53 c.484A>T p.I162F EX5 0.7% missensePE276 EGFR c.2240_2254delTAAGAGAAGCAACATp.L747_T751del EX19 15.5% cds-delPE243 TP53 c.395A>G p.K132R EX5 36.3% missensePE207 MSH3 c.1141A[8>7] p.K383Rfs*32 EX7 23.6% frameshiftPE431 EGFR c.2390G>C p.C797S EX20 0.7% missensePE394 SLX4 c.2902G>C p.E968Q EX12 10.5% missensePE428 LRP1B c.3601G>A p.E1201K EX23 4.5% missensePE500 EP300 c.1301C>T p.P434L EX6 12.5% missensePE542 ARHGAP35 c.3962T>C p.M1321T EX4 4.1% missensePE527 MLL2 c.10074delT p.S3358Rfs*34 EX34 28.9% frameshiftPE507 MLL3 c.365C>A p.S122Y EX3 1.2% missensePE585 TP53 c.626_627delGA p.R209Kfs*6 EX6 74.9% frameshiftPE318 NFE2L2 c.1122_1140delTGATTCTGAAGTGGAAGAGp.D375*fs*1 EX5E 16.0% frameshiftPE593 MSH2 c.1255C>A p.Q419K EX7 12.8% missensePE713 ATM c.949G>C p.D317H EX8 4.1% missensePE767 KEAP1 c.701G>A p.R234Q EX3 1.2% missensePE717 SMAD4 c.431C>G p.S144* EX4 3.4% nonsensePE755 WNK1 c.1243_1245delATGp.M415del EX4 7.9% cds-delPE819 TP53 c.1006G>T p.E336* EX10 2.5% nonsensePE686 CYP2D6 c.1398C>A p.F466L EX9E 4.7% missensePE795 EGFR c.2239_2252delTTAAGAGAAGCAACinsCAp.L747_T751delinsQEX19 16.0% cds-indelPE685 CUL3 c.2113G>C p.A705P EX15 0.6% missensePE810 TP53 c.615T[3>2] p.L206Wfs*41 EX6 15.9% frameshiftPE891 BCOR c.3721A>G p.T1241A EX8 3.6% missensePE220 BRCA2 c.9098C>G p.T3033S EX23 23.5% missense

PE222 DNMT3A c.804delT p.D268Efs*48 EX7 0.5% frameshiftPE336 PIK3CA c.1585G>A p.E529K EX10 21.5% missensePE324 LRP1B c.11169C>A p.C3723* EX73 23.0% nonsensePE207 NTRK1 c.658C>T p.R220W EX6 1.1% missensePE363 GRM3 c.374AT[2>1] p.M126Vfs*3 EX2 11.2% frameshiftPE332 TP53 c.686_687delGT p.C229Yfs*10 EX7 33.5% frameshiftPE399 FMN2 c.2866C>A p.P956T EX5 9.3% missensePE461 ARID1A c.3406+1G>A . IVS12 1.1% splice-5PE417 STAT1 c.1868G>C p.G623A EX21 13.5% missensePE555 BRAF c.1799T>A p.V600E EX15 3.4% missensePE524 EGFR c.2573T>G p.L858R EX21 5.2% missensePE520 PALB2 c.3355C>G p.L1119V EX13E 25.5% missensePE531 SETD2 c.3533delC p.S1178Lfs*13 EX3 14.2% frameshiftPE528 ARID1A c.1469C>T p.P490L EX3 0.6% missensePE594 NCOR1 c.1703C>T p.T568I EX16 29.5% missensePE639 MS4A1 c.247A>G p.T83A EX4 6.9% missensePE509 RB1 c.1351C>A p.R451S EX14 65.2% missensePE572 ATR c.693G>T p.Q231H EX4 13.4% missensePE746 CHI3L1 c.379C>G p.L127V EX5 48.4% missensePE480 APC c.4479G[2>1] p.E1494Kfs*13 EX16E 13.7% frameshiftPE644 SETD2 c.3343G>T p.E1115* EX3 20.0% nonsensePE783 RB1 c.1388C>A p.S463* EX14 23.6% nonsensePE679 TP53 c.464C>A p.T155N EX5 11.7% missensePE662 BRAF c.1790T>G p.L597R EX15 3.3% missensePE749 CDKN2A c.298_313delGCCGGGGCGCGGCTGGp.A100Tfs*41 EX2 8.0% frameshiftPE857 IDH2 c.786C>G p.F262L EX6 30.1% missensePE882 EPHA2 c.1621G>C p.V541L EX8 1.3% missensePE828 B2M c.2T>A p.0? EX1 5.6% init-lossPE693 TP53 c.379T>A p.S127T EX5 35.2% missensePE270 EPHA3 c.2075-2delA . IVS11 21.4% splice-3PE351 EGFR c.2236_2250delGAATTAAGAGAAGCAp.E746_A750del EX19 16.7% cds-delPE378 FCGR2A c.317G>T p.G106V EX3 6.2% missensePE344 EGFR c.2240_2257delTAAGAGAAGCAACATCTCp.L747_P753delinsSEX19 14.4% cds-indelPE386 BRAF c.1799T>A p.V600E EX15 8.1% missensePE420 FLT4 c.544G>T p.E182* EX5 1.1% nonsensePE503 EGFR c.2369C>T p.T790M EX20 1.3% missensePE506 SETD2 c.3871G>T p.E1291* EX3 90.6% nonsensePE358 EGFR c.2369C>T p.T790M EX20 31.3% missensePE582 SMARCA4 c.1342C>T p.R448C EX8 29.2% missensePE660 FLT3 c.466T>C p.F156L EX4 1.2% missensePE705 PTEN c.635-1G>A . IVS6 40.7% splice-3PE689 CBL c.948G>C p.Q316H EX6 1.0% missensePE478 NF2 c.863C>G p.S288* EX9 2.8% nonsensePE757 CDKN2A c.83_100delTGCGGGCGCTGCTGGAGGp.V28_E33del EX1 28.9% cds-delPE724 EGFR c.2235_2249delGGAATTAAGAGAAGCp.E746_A750del EX19 19.8% cds-delPE925 IRS2 c.1138G>T p.A380S EX1 4.1% missensePE816 FANCM c.99G>C p.Q33H EX1 4.6% missensePE343 LRP1B c.2798C>A p.S933Y EX18 5.0% missensePE322 PTEN c.388C>T p.R130* EX5 69.6% nonsensePES121 EGFR c.2156G>C p.G719A EX18 13.2% missensePES121 EGFR c.2156G>C p.G719A EX18 16.6% missensePE511 NSD1 c.8008G>A p.D2670N EX23E 6.2% missensePE638 NOTCH4 c.1055G>C p.C352S EX6 2.6% missensePE639 SMAD4 c.1052A>C p.D351A EX9 1.8% missense

PE572 PCK1 c.1289G>T p.G430V EX8 21.5% missensePE737 GATA3 c.628G>A p.G210R EX3 1.4% missensePE690 TGFBR1 c.962T>G p.V321G EX5 1.3% missensePE622 DOT1L c.4370C>T p.S1457F EX27 1.0% missensePE758 ATRX c.995A>G p.D332G EX9 38.3% missensePE880 EGFR c.2573T>G p.L858R EX21 28.4% missensePE858 RBM10 c.2351A>G p.D784G EX19 16.6% missensePE938 RB1 c.1421+2T>A . IVS15 21.3% splice-5PE890 ARAF c.563G>A p.R188H EX7 1.5% missensePE913 EXT1 c.1088G>A p.G363E EX3 4.2% missensePE303 BRAF c.1799T>A p.V600E EX15 8.3% missensePE207 STAT3 c.1360C>A p.L454I EX15 18.2% missensePE416 EGFR c.2369C>T p.T790M EX20 5.2% missensePE453 TP53 c.742C>T p.R248W EX7 8.9% missensePE503 BRIP1 c.602A>C p.K201T EX6 14.0% missensePE487 TP53 c.578A>G p.H193R EX6 89.9% missensePE546 SERPINB3 c.1129A>G p.K377E EX8E 0.9% missensePES121 CDK4 c.602G>T p.G201V EX5 0.5% missensePE214 TP53 c.395A>G p.K132R EX5 21.3% missensePE584 NOTCH1 c.6679C>G p.P2227A EX34E 4.0% missensePE637 SMAD4 c.1521A>T p.K507N EX12E 0.7% missensePE601 DNMT3A c.691C>T p.Q231* EX7 1.0% nonsensePE728 STK11 c.520C>T p.H174Y EX4 0.9% missensePE668 TP53 c.325T>A p.F109I EX4 43.7% missensePE659 PPM1D c.1619delA p.E540Gfs*7 EX6E 0.8% frameshiftPE745 SMAD4 c.367delT p.C123Vfs*6 EX3 20.5% frameshiftPE679 LRP1B c.8362C>A p.P2788T EX52 8.4% missensePE786 RBM10 c.1241_1254delAGCTCTCCACCATCp.Q414Rfs*27 EX10 8.7% frameshiftPE792 MEF2B c.268C>T p.R90W EX4 2.7% missensePE878 ZFHX3 c.8398C>G p.L2800V EX9 2.9% missensePE876 TP53 c.841G>C p.D281H EX8 4.1% missensePES121 ACIN1 c.822GGA[6>5] p.E270[12>11] 0.6% cds-delPE424 MYD88 c.415G>A p.E139K EX2 1.4% missensePE436 PIK3C2G c.4180G>A p.E1394K EX31 1.6% missensePE374 SOX9 c.770G>A p.R257H EX3E 1.0% missensePE441 ARID1B c.1978G>A p.G660R EX5 11.7% missensePE397 MDM2 c.464C>T p.S155F EX7 0.8% missensePE528 OR5L2 c.127C>A p.L43M EX1E 4.1% missensePE421 ATR c.5104C>G p.L1702V EX29 9.8% missensePE587 EGFR c.2235_2249delGGAATTAAGAGAAGCp.E746_A750del EX19 9.8% cds-delPE611 MYD88 c.415G>A p.E139K EX2 1.2% missensePE737 SDHA c.770+1G>T . IVS6 2.0% splice-5PE690 RINT1 c.545A>G p.N182S EX5 18.2% missensePE758 ARID2 c.556G>C p.E186Q EX5 2.1% missensePE858 NF1 c.683C>T p.P228L EX7 10.1% missensePE703 LRP1B c.1856G>A p.G619D EX12 19.8% missensePE913 RICTOR c.1030G>A p.E344K EX12 6.1% missensePE917 CDKN2A c.151-17_158delTCTGTTCTCTCTGGCAGGTCATGAT. IVS1-EX2 4.9% spanPE303 NOTCH4 c.3142G>C p.D1048H EX20 5.5% missensePE207 GRIN2A c.1006C>A p.P336T EX3 18.1% missensePE204 EGFR c.2369C>T p.T790M EX20 8.2% missensePE416 BRAF c.1799T>A p.V600E EX15 1.5% missensePES121 NF1 c.7501A>T p.M2501L EX50 0.4% missensePE493 EGFR c.2240_2257delTAAGAGAAGCAACATCTCp.L747_P753delinsSEX19 4.8% cds-indel

PE442 TP53 c.644G>T p.S215I EX6 87.4% missensePE584 NOTCH1 c.6632C>T p.S2211L EX34E 4.4% missensePE664 TP53 c.413C[2>1] p.K139Rfs*31 EX5 17.8% frameshiftPE586 PTEN c.94ATT[2>1] p.I32[2>1] EX2 46.6% cds-delPE701 FAT1 c.2626G>A p.V876I EX2 37.8% missensePE354 EGFR c.2573T>G p.L858R EX21 24.1% missensePE728 CHEK2 c.1448A>G p.H483R EX13 2.1% missensePE679 ERBB4 c.3200G>T p.R1067L EX27 12.1% missensePE341 PBRM1 c.4426C[5>4] p.P1477Lfs*12 EX29 28.9% frameshiftPE402 DIAPH1 c.791C>G p.S264C EX8 41.7% missensePE400 FOXA1 c.493A>C p.S165R EX2 15.7% missensePE429 ELMO1 c.1579G>A p.D527N EX17 1.1% missensePE433 EGFR c.2573T>G p.L858R EX21 35.6% missensePE464 EGFR c.2239_2256delTTAAGAGAAGCAACATCTp.L747_S752del EX19 9.3% cds-delPE448 EGFR c.2240_2259delTAAGAGAAGCAACATCTCCGinsCCp.L747_P753delinsSEX19 37.0% cds-indelPE443 DNMT3A c.2216A>C p.H739P EX19 0.3% missensePE457 RINT1 c.1445T>C p.F482S EX10 0.7% missensePE456 IDH1 c.394C>T p.R132C EX4 12.8% missensePE547 SMARCA4 c.1111G>T p.E371* EX6 5.0% nonsensePE474 PC c.2552C>T p.T851M EX19 0.5% missensePE544 EGFR c.2232C>G p.I744M EX19 82.1% missensePE586 MTOR c.6801C>G p.I2267M EX48 19.3% missensePE588 NF1 c.205A>G p.R69G EX3 29.3% missensePE553 PALB2 c.1660G>A p.E554K EX4 19.9% missensePE440 TP53 c.946C[4>5] p.Q317Pfs*20 EX9 60.0% frameshiftPE646 ITK c.1230G>T p.M410I EX12 7.4% missensePE669 LRP1B c.2307T>A p.S769R EX14 6.8% missensePE689 HIST1H2AGc.163G>C p.V55L EX1E 2.0% missensePE780 EGFR c.2369C>T p.T790M EX20 11.5% missensePE793 FCGR1A c.710G>A p.R237Q EX5 6.1% missensePE563 TET2 c.5636A>T p.E1879V EX11E 69.7% missensePE885 EGFR c.2369C>T p.T790M EX20 7.5% missensePE900 EGFR c.2235_2249delGGAATTAAGAGAAGCp.E746_A750del EX19 2.2% cds-delPE903 NCOR1 c.4171G>A p.D1391N EX31 13.5% missensePE223 ERBB2 c.2329G>T p.V777L EX20 1.3% missensePE231 EGFR c.2369C>T p.T790M EX20 12.7% missensePE269 PDGFRA c.646C>A p.L216I EX5 2.1% missensePE274 EGFR c.2235_2249delGGAATTAAGAGAAGCp.E746_A750del EX19 6.9% cds-delPE252 CDH1 c.760G>A p.D254N EX6 40.9% missensePE349 GNAQ c.1070A>G p.N357S EX7 10.3% missensePE341 TOP2A c.1897A>T p.R633* EX16 2.7% nonsensePE287 SETD2 c.310C>G p.P104A EX3 4.0% missensePE402 DNM2 c.2365C>G p.P789A EX20 2.4% missensePE395 CSMD1 c.8590G>T p.V2864F EX56 11.4% missensePE429 PIK3C2G c.4180G>A p.E1394K EX31 1.4% missensePE452 PIK3C2G c.4180G>A p.E1394K EX31 0.9% missensePE368 NOTCH1 c.436_450delTCCAACCCCTGCGCCp.S146_A150del EX4 2.0% cds-delPE323 ZFHX3 c.9748A>T p.K3250* EX10E 29.8% nonsensePE388 PIK3C2G c.4180G>A p.E1394K EX31 1.9% missensePE383 TP53 c.833C>G p.P278R EX8 41.9% missensePE457 GNG2 c.106G>T p.D36Y EX4E 4.8% missensePE396 RBM10 c.2437G>T p.E813* EX20 5.1% nonsensePE550 HGF c.866-1G>T . IVS7 1.8% splice-3PE578 LRP1B c.4217C>G p.S1406C EX26 19.3% missense

PE552 PIK3CG c.3151A>T p.I1051F EX11E 21.4% missensePE551 MLL3 c.5012G>A p.W1671* EX34 4.0% nonsensePE646 TP53 c.811G>A p.E271K EX8 4.4% missensePE384 PTPRD c.18GCT[5>4] p.L7[6>5] EX12 0.5% cds-delPE698 BRD4 c.2663C>T p.P888L EX14 1.8% missensePE793 IRS2 c.3842A>G p.K1281R EX1 10.3% missensePE768 DNMT3A c.2195T>C p.F732S EX19 2.8% missensePE753 CDK13 c.1583G>A p.S528N EX2 0.5% missensePE902 FANCM c.5581A>T p.M1861L EX21 2.3% missensePE885 NF2 c.1332AG[3>2] p.R446Efs*48 EX12 10.4% frameshiftPE892 JUN c.622C>T p.P208S EX1E 4.0% missensePE223 KRAS c.38G>A p.G13D EX2 2.6% missensePE207 PTCH1 c.881G>A p.R294H EX6 2.3% missensePE313 FXR1 c.1319G>A p.R440K EX14 2.1% missensePE338 PRKAA1 c.590G>C p.R197T EX6 37.0% missensePE339 NF1 c.3089C>G p.S1030* EX23 6.0% nonsensePE408 TP53 c.320_328delACGGTTTCCp.Y107_R110delinsCEX4 2.8% cds-indelPE412 EGFR c.2573T>G p.L858R EX21 65.2% missensePE428 TP53 c.614A>G p.Y205C EX6 22.9% missensePE555 ELF3 c.220G>A p.D74N EX3 4.7% missensePE525 TP53 c.818G>A p.R273H EX8 20.5% missensePE520 MET c.3029-731delA . IVS14 13.1% intronPE594 POLE c.2089C>G p.P697A EX19 1.5% missensePE516 POLE c.848T>C p.L283P EX9 32.3% missensePE561 STK11 c.647C>T p.S216F EX5 5.2% missensePE509 ARID1B c.986C>G p.A329G EX1 18.8% missensePE514 APC c.3930_3964delGATTGGAACTAGGTCAGCTGAAGATCCTGTGAGCGinsTp.K1310Nfs*94 EX16E 1.5% frameshiftPE572 MYCL1 c.290G>A p.G97E EX2 21.2% missensePE634 RBM10 c.1996C>T p.Q666* EX17 8.0% nonsensePE849 TP53 c.814G>T p.V272L EX8 4.2% missensePE491 FANCD2 c.826G>C p.E276Q EX11 59.3% missensePE616 EGFR c.2235_2249delGGAATTAAGAGAAGCp.E746_A750del EX19 13.1% cds-delPE875 TP53 c.844C>T p.R282W EX8 11.4% missensePE631 TP53 c.390CAA[2>1] p.N131del EX5 52.9% cds-delPE270 TP53 c.831T>A p.C277* EX8 13.2% nonsensePE223 SMAD4 c.1000C>T p.Q334* EX9 1.2% nonsensePE350 FAT3 c.12677G>A p.G4226E EX23 2.9% missensePE311 PPM1D c.1449T[3>4] p.L484Ffs*5 EX6 1.2% frameshiftPE352 ARHGAP35 c.196C>T p.R66* EX1 19.0% nonsensePE362 TP53 c.783-2A>C . IVS7 8.1% splice-3PE356 NQO1 c.659C>A p.P220Q EX6 86.9% missensePE333 MED12 c.1841A>G p.Y614C EX13 0.8% missensePE394 PIK3CA c.1633G>A p.E545K EX10 23.1% missensePE447 EGFR c.2236_2250delGAATTAAGAGAAGCAp.E746_A750del EX19 12.9% cds-delPE500 ABCA13 c.10999C>T p.L3667F EX33 7.0% missensePE539 ZFHX3 c.223G>T p.A75S EX2 36.6% missensePE593 APC c.1685C>T p.T562M EX14 17.6% missensePE644 LRP1B c.5673_5674delAAinsCCp.I1892L EX35 0.5% missensePE728 TET2 c.2469G>A p.M823I EX3 3.5% missensePE686 TP53 c.783-4_786delGTAGTGGT. IVS7-EX8 24.0% spanPE565 MLL3 c.11837C>T p.P3946L EX46 1.0% missensePE770 EXT1 c.414C>G p.I138M EX1 2.3% missensePE651 EGFR c.2235_2249delGGAATTAAGAGAAGCp.E746_A750del EX19 35.5% cds-delPE840 NOTCH1 c.5429A>G p.Q1810R EX29 9.4% missense

PE850 EGFR c.2240_2254delTAAGAGAAGCAACATp.L747_T751del EX19 15.2% cds-delPE785 JAK3 c.362G>A p.R121H EX4 26.9% missensePE876 EPHB2 c.1785C>G p.I595M EX10 2.6% missensePE656 ROBO3 c.1063G>A p.D355N EX7 0.9% missensePE895 TP53 c.733G>A p.G245S EX7 87.7% missensePE935 TP53 c.473G>T p.R158L EX5 23.0% missensePES130 MLL4 c.1096G>T p.E366* EX3 5.9% nonsensePE212 FAT2 c.5111C>G p.S1704C EX9 1.4% missensePE268 IL7R c.835C>A p.H279N EX7 1.4% missensePE260 MLL c.9605C>T p.S3202L EX27 0.9% missensePE207 BAP1 c.166C>T p.R56C EX4 8.1% missensePE606 EGFR c.2239_2256delTTAAGAGAAGCAACATCTp.L747_S752del EX19 38.0% cds-delPE339 MLL3 c.8518G>T p.E2840* EX38 8.3% nonsensePE329 PIK3CA c.2872C>A p.Q958K EX20 75.4% missensePE320 MET c.3029-1351A>C . IVS14 1.1% intronPE434 KLF4 c.1237C>G p.L413V EX4 10.1% missensePE450 TP53 c.451C>T p.P151S EX5 21.5% missensePE388 FOXP1 c.1909G>A p.E637K EX17E 8.1% missensePE458 BRD4 c.1063A>C p.K355Q EX6 7.6% missensePE492 TP53 c.724T>A p.C242S EX7 27.2% missensePE359 TERT c.-58-u66C>T . . 16.5% promoterPE594 EGFR c.2317C>T p.H773Y EX20 15.6% missensePE764 EGFR c.2573T>G p.L858R EX21 55.3% missensePE718 POT1 c.703G>T p.V235F EX10 4.4% missensePE777 EGFR c.2235_2249delGGAATTAAGAGAAGCp.E746_A750del EX19 15.9% cds-delPE771 PTEN c.325delG p.D109Tfs*4 EX5 8.7% frameshiftPE832 TOP2A c.1898G>C p.R633T EX16 1.6% missensePE849 EPHA3 c.2647C>T p.R883W EX15 3.6% missensePE710 NOTCH1 c.7292G>T p.R2431L EX34E 35.8% missensePE620 BRCA1 c.5117G>A p.G1706E EX17 8.6% missensePE875 FAT2 c.12091G>T p.G4031* EX22 1.2% nonsensePE900 BCOR c.8C>T p.S3L EX2 1.1% missensePES130 JAK1 c.1979A>G p.D660G EX14 1.8% missensePE308 RICTOR c.2079C>A p.N693K EX22 1.7% missensePE303 MLL3 c.1616C>G p.T539S EX11 4.8% missensePE301 TP53 c.1051A>T p.K351* EX10 10.1% nonsensePE207 TCF7L2 c.1480C[6>5] p.S496Pfs*5 EX14 16.6% frameshiftPE416 KDM5C c.3826G>A p.E1276K EX23 0.5% missensePE413 EGFR c.2153T>A p.L718Q EX18 10.8% missensePE558 RICTOR c.2057T>G p.L686R EX22 16.7% missensePE596 TP53 c.1039G>A p.A347T EX10 1.2% missensePE694 NF1 c.2792_2798delCAATGCTp.P931Hfs*5 EX21 18.4% frameshiftPE857 HIST1H2BJc.69G>C p.Q23H EX1E 28.7% missensePE807 LRP1B c.7118G>A p.R2373H EX43 30.0% missensePE847 TP53 c.536A>G p.H179R EX5 3.1% missensePE904 EGFR c.2303G>T p.S768I EX20 30.8% missensePE922 APC c.6887G>C p.S2296T EX16E 19.6% missensePE299 FLCN c.247G>A p.E83K EX4 0.5% missensePE207 EXT2 c.98T>C p.V33A EX2 9.9% missensePE317 MMP11 c.274C>T p.R92C EX2 2.9% missensePE459 BRSK1 c.1253G>A p.R418Q EX12 1.3% missensePE543 PIK3CA c.1633G>C p.E545Q EX10 5.4% missensePE518 CSF1R c.316C>T p.R106W EX4 2.4% missensePE594 MAP3K1 c.152G>A p.G51D EX1 14.1% missense

PE580 IRF2 c.744_745insA p.R249Tfs*10 EX9E 10.8% frameshiftPE634 MSH6 c.2617G>C p.G873R EX4 5.8% missensePE784 SMARCB1 c.1130G>A p.R377H EX9E 26.7% missensePE744 ROCK1 c.1014C>G p.F338L EX9 14.4% missensePE812 SMARCA4 c.2729C>T p.T910M EX19 44.0% missensePE684 TET2 c.2686C>A p.L896I EX3 2.3% missensePE840 ACIN1 c.1940_1941insTCGTACp.S647_A648insRTEX6 0.7% cds-insPE889 VTCN1 c.515G>A p.R172Q EX4 1.3% missensePE653 CREBBP c.1397A>T p.N466I EX6 31.1% missensePE861 FAT1 c.8362G>A p.E2788K EX10 0.5% missensePE914 EPHA3 c.2656G>A p.G886S EX15 19.0% missensePE655 SETD2 c.995C>G p.S332* EX3 39.5% nonsensePE223 TP53 c.945dupT p.P316Sfs*21 EX9 15.9% frameshiftPE207 CTNNA1 c.2350G>A p.A784T EX17 3.0% missensePE280 DNMT3A c.2645G>A p.R882H EX23 0.9% missensePE313 LRP1B c.3712T>A p.S1238T EX23 3.9% missensePE339 TP53 c.1031T>G p.L344R EX10 38.0% missensePE332 EPHA3 c.587G>C p.R196T EX3 24.8% missensePE408 NAV3 c.1813A>T p.S605C EX8 1.7% missensePE429 FAT1 c.10548+1G>T . IVS18 8.9% splice-5PE428 FANCA c.2080G>A p.D694N EX23 5.4% missensePE525 ABL1 c.221C>G p.A74G EX2 9.8% missensePE573 FMN2 c.2829_2861delTCCTCCGCCCCCTCTACCCGGAGCGGCAATACCp.A952_A962del EX5 18.3% cds-delPE594 TBX3 c.52C>T p.H18Y EX1 23.1% missensePE516 FYN c.980A>G p.Y327C EX7 59.6% missensePE771 EGFR c.2239_2248delinsCp.L747_A750delinsPEX19 7.6% cds-indelPE684 B2M c.1A>T p.0? EX1 66.5% init-lossPE849 CDH11 c.1070A>C p.K357T EX8 1.0% missensePE491 LRP1B c.8402C>G p.P2801R EX53 20.9% missensePE875 GRIN2A c.706G>C p.A236P EX3 0.8% missensePE631 PTEN c.464A>G p.Y155C EX5 41.7% missensePE693 ERBB3 c.2854G>C p.E952Q EX24 1.6% missensePE342 BRAF c.1799T>A p.V600E EX15 10.4% missensePE346 FBXW7 c.1099C>T p.R367* EX7 4.6% nonsensePE404 HSP90AB1 c.1997_1998delCTinsTGp.S666L EX11 1.1% missensePE364 SERPINA7 c.405C>A p.F135L EX2 13.4% missensePE406 DNMT3A c.1644G>A p.M548I EX14 4.0% missensePE442 FAT2 c.10870C>A p.Q3624K EX18 3.6% missensePE411 NOTCH3 c.3364G>C p.D1122H EX21 13.7% missensePE720 MEN1 c.1234C>T p.H412Y EX9 14.6% missensePE643 EGFR c.2573T>G p.L858R EX21 6.3% missensePE689 STAT3 c.46G>A p.E16K EX2 1.1% missensePE648 CDKN2A c.43TGGC[2>1] p.L16Pfs*9 EX1 6.6% frameshiftPE682 FLT3 c.2029C>A p.L677M EX16 1.3% missensePE722 STAG2 c.3658G>A p.E1220K EX33 10.1% missensePE867 JAK1 c.3364G>T p.D1122Y EX24 3.6% missensePE866 TP53 c.310C>T p.Q104* EX4 7.5% nonsensePE829 TET2 c.4075C>T p.R1359C EX9 0.6% missensePE735 TP53 c.444T[2>1] p.S149Pfs*21 EX5 60.0% frameshiftPE863 RB1 c.717T>A p.Y239* EX7 90.7% nonsensePE896 MET c.3028+2T>C . IVS14 2.3% splice-5PE934 GATA3 c.325G>A p.A109T EX3 3.3% missensePE907 EGFR c.2239_2248delinsCp.L747_A750delinsPEX19 0.3% cds-indelPE918 ANKRD30A c.1559C>T p.S520L EX13 5.1% missense

PE250 FAP c.713A>T p.Y238F EX9 5.8% missensePE254 PIK3CA c.1633G>A p.E545K EX10 0.5% missensePE276 PRDM16 c.2773_2774insG p.L925Rfs*58 EX11 13.6% frameshiftPE243 TP53 c.455C>T p.P152L EX5 35.1% missensePE207 FAT1 c.3846C[4>3] p.N1284Mfs*84 EX5 21.8% frameshiftPE431 AR c.188A>T p.Q63L EX1 4.3% missensePE394 SLX4 c.2848G>C p.E950Q EX12 10.2% missensePE465 EGFR c.2573T>G p.L858R EX21 58.4% missensePE500 EP300 c.2705C>T p.P902L EX14 5.3% missensePE442 MS4A1 c.668C>A p.P223H EX7 16.6% missensePE507 TBX3 c.36_37delGAinsTTp.T13S EX1 11.3% missensePE318 IMPG1 c.230G>T p.G77V EX2 22.0% missensePE593 MLL3 c.2222T>C p.M741T EX14 12.6% missensePE654 GAB2 c.479C>T p.A160V EX3 18.2% missensePE613 MED12 c.1850C>G p.T617S EX13 49.2% missensePE673 AKT2 c.377C>T p.S126F EX5 16.7% missensePE713 MLL2 c.6019G>A p.E2007K EX28 19.3% missensePE728 CDH23 c.6847G>C p.V2283L EX50 0.7% missensePE717 NOTCH3 c.5158G>A p.D1720N EX28 2.9% missensePE788 APC c.7759G>A p.E2587K EX16E 6.3% missensePE745 ATR c.6109G>T p.D2037Y EX36 16.4% missensePE794 TP53 c.707A>G p.Y236C EX7 29.9% missensePE800 MCL1 c.472G>T p.G158W EX1 13.0% missensePE891 KDM5C c.2332C>T p.R778* EX16 3.3% nonsensePES130 RHOA c.454G>T p.G152C EX5 0.5% missensePE308 CDK12 c.2953G>A p.E985K EX10 0.6% missensePE216 SMAD4 c.1572G>T p.W524C EX12 3.7% missensePE303 PDGFRA c.1147C>A p.R383S EX8 4.1% missensePE207 PAX5 c.64C>A p.Q22K EX2 12.5% missensePE204 ZFHX3 c.9304G>A p.D3102N EX9 4.1% missensePE413 ATM c.2381G>C p.S794T EX16 17.5% missensePE485 FAM5C c.1254C>G p.S418R EX8E 16.9% missensePE558 TP53 c.149T[2>1] p.I50Mfs*73 EX4 52.3% frameshiftPE513 EGFR c.2369C>T p.T790M EX20 65.8% missensePE594 ELF3 c.281G>T p.R94L EX3 2.2% missensePE580 PMS1 c.1649C>T p.S550L EX9 2.4% missensePE629 EGFR c.2154_2155delGGinsTTp.G719C EX18 79.1% missensePE642 PIK3CA c.1258T>C p.C420R EX8 12.4% missensePE696 MDM4 c.1100C>T p.S367L EX11E 0.6% missensePE825 KIT c.1643T>C p.L548S EX10 4.7% missensePE807 EPHA7 c.685G>C p.E229Q EX3 28.0% missensePE922 APC c.7468G>A p.D2490N EX16E 19.9% missensePE206 MAP3K1 c.1643A>T p.Q548L EX9 0.8% missensePE369 EXT1 c.1969A>C p.M657L EX10 5.0% missensePE392 SBDS c.428C>T p.S143L EX3 5.4% missensePE205 FGFR3 c.1262G>A p.R421Q EX9 1.5% missensePE421 EGFR c.2573T>G p.L858R EX21 92.5% missensePE687 SIN3A c.674A>G p.Q225R EX5 2.4% missensePE748 HOXA3 c.380C>T p.P127L EX3 13.7% missensePE834 GRIN2A c.2458G>A p.V820I EX12 0.5% missensePE839 RBM10 c.2776delA p.I926Lfs*25 EX23 37.7% frameshiftPE872 PTPRD c.2911C>A p.L971I EX28 28.7% missensePE937 APC c.7389A>T p.E2463D EX16E 0.7% missensePE206 PIK3CA c.1624G>A p.E542K EX10 1.8% missense

PE300 EGFR c.2240_2259delTAAGAGAAGCAACATCTCCGinsCCp.L747_P753delinsSEX19 5.1% cds-indelPE389 BCOR c.4165G>A p.D1389N EX9 0.5% missensePES121 TP53 c.464C>A p.T155N EX5 1.2% missensePE520 PIK3CG c.928C>A p.P310T EX2 12.0% missensePE687 CDKN2A c.212A>G p.N71S EX2 2.0% missensePE748 FLG c.8556G>T p.R2852S EX3E 2.5% missensePE840 TP53 c.548C>G p.S183* EX5 22.5% nonsensePE872 TET2 c.1912A>G p.M638V EX3 21.6% missensePE913 AR c.1853G>A p.R618Q EX3 5.4% missensePE342 TBX3 c.2035G>A p.A679T EX8 1.0% missensePE346 APC c.4099C>T p.Q1367* EX16 5.0% nonsensePE404 BRAF c.1094C>T p.S365L EX8 3.7% missensePE455 NOTCH1 c.3473C>G p.A1158G EX21 1.0% missensePE489 EGFR c.2573T>G p.L858R EX21 3.0% missensePE406 NOTCH4 c.2600A>G p.H867R EX17 2.9% missensePE673 EPHA3 c.501G>C p.E167D EX3 18.1% missensePE647 TP53 c.991C>T p.Q331* EX9 19.2% nonsensePE484 ARID1B c.124_126delGCGinsTCCp.A42S EX1 1.3% missensePE689 SMARCA4 c.2044C>G p.L682V EX14 1.0% missensePE761 EGFR c.2369C>T p.T790M EX20 16.2% missensePE682 ERCC4 c.290G>A p.R97H EX2 1.4% missensePE680 HRAS c.304C>T p.R102W EX4 13.4% missensePE765 TP53 c.837_843dupGAGAGACp.R282Efs*26 EX8 1.1% frameshiftPE657 EGFR c.2573T>G p.L858R EX21 5.5% missensePE714 NRXN1 c.526G>A p.E176K EX2 1.9% missensePE867 EGFR c.2573T>G p.L858R EX21 25.4% missensePE829 PALB2 c.698T>C p.V233A EX4 0.7% missensePE735 KEAP1 c.445G>T p.E149* EX2 50.3% nonsensePE900 NCOR1 c.3022C>T p.Q1008* EX23 1.0% nonsensePE863 TP53 c.844C>T p.R282W EX8 68.6% missensePE891 SETD2 c.6625T[2>3] p.L2209Ffs*37 EX15 4.2% frameshiftPE896 TP53 c.733G>A p.G245S EX7 0.5% missensePE934 CCND2 c.119A>C p.Q40P EX1 1.7% missensePE918 ARID2 c.2809C>T p.Q937* EX15 22.8% nonsensePE222 SETD2 c.3136A>G p.N1046D EX3 9.3% missensePE218 ALK c.3604G>A p.G1202R EX23 3.3% missensePE302 PTEN c.517C>T p.R173C EX6 86.8% missensePE305 CTNNB1 c.110C>T p.S37F EX3 0.5% missensePE310 CDKN2A c.45G>A p.W15* EX1 9.8% nonsensePE424 FGFR1 c.160C>T p.R54C EX3 1.0% missensePE448 FAM175A c.874G>A p.E292K EX9E 1.4% missensePE534 TP53 c.818G>A p.R273H EX8 4.8% missensePE523 JAK2 c.433C>G p.L145V EX5 24.2% missensePE482 TP53 c.374C>G p.T125R EX4 34.1% missensePE421 C11orf30 c.1682C>G p.S561C EX11 9.2% missensePE588 CHD1L c.2515C>G p.H839D EX22 13.6% missensePE440 KIT c.1255G>A p.D419N EX8 5.1% missensePE737 TP53 c.461G>T p.G154V EX5 89.2% missensePE687 PCK1 c.611-1G>T . IVS4 1.5% splice-3PE650 MLL3 c.1129C>A p.P377T EX8 4.5% missensePE812 CTNNB1 c.98C>T p.S33F EX3 7.1% missensePE826 PDCD1 c.328G>A p.V110M EX2 18.8% missensePE916 PTPRD c.5284C>T p.R1762W EX43 2.9% missensePE591 KRAS c.183A>C p.Q61H EX3 27.4% missense

PE938 SMAD4 c.1590_1601delCCGGGCCCTCCAp.H530_L533del EX12E 0.7% cds-delPE833 EGFR c.2389T>A p.C797S EX20 9.2% missensePES130 EGFR c.2573T>G p.L858R EX21 16.9% missensePE308 ARID1A c.6806C>G p.S2269* EX20 0.8% nonsensePE303 EXT2 c.1594G>A p.E532K EX10 4.8% missensePE301 KRAS c.34G>T p.G12C EX2 13.4% missensePE207 LRP1B c.4264A[4>3] p.T1423Lfs*8 EX26 16.9% frameshiftPE204 MLL2 c.2264G>T p.R755L EX10 1.3% missensePE416 TP53 c.821T>G p.V274G EX8 3.2% missensePE503 BTK c.619G>A p.A207T EX8 3.6% missensePE493 NOTCH3 c.2654C>G p.A885G EX17 6.1% missensePE558 FUBP1 c.315C>G p.Y105* EX5 20.5% nonsensePE584 MEN1 c.1108G>A p.D370N EX8 6.4% missensePE694 TP53 c.743G>A p.R248Q EX7 35.1% missensePE840 ATRX c.3854C>T p.S1285F EX11 18.7% missensePE807 ALK c.3512T>C p.I1171T EX22 62.1% missensePE847 EGFR c.2573T>G p.L858R EX21 1.8% missensePE904 EGFR c.2155G>A p.G719S EX18 32.1% missensePE270 EXT1 c.2179G>A p.V727I EX11 13.8% missensePE223 EGFR c.2236_2250delGAATTAAGAGAAGCAp.E746_A750del EX19 0.9% cds-delPE251 GNAS c.602G>A p.R201H EX8 1.2% missensePE350 APC c.2853T>G p.Y951* EX16 6.1% nonsensePE245 CACNA1E c.403T[4>2] p.F135Lfs*3 EX3 7.1% frameshiftPE352 NOS3 c.2002G>A p.D668N EX17 27.9% missensePE362 PIK3C2G c.4180G>A p.E1394K EX31 2.2% missensePE333 EGFR c.2155G>A p.G719S EX18 1.0% missensePE378 EP300 c.2419A>T p.I807L EX14 6.8% missensePE460 TP53 c.817C>T p.R273C EX8 57.6% missensePE447 TP73 c.691A>T p.T231S EX6 9.5% missensePE490 EGFR c.185T>G p.L62R EX2 11.2% missensePE500 GABRA6 c.1201C>A p.P401T EX9E 4.1% missensePE539 CREBBP c.6334C>T p.Q2112* EX31E 23.1% nonsensePE442 LYN c.1090A>G p.I364V EX11 15.8% missensePE593 ALK c.4208C>T p.P1403L EX29E 20.3% missensePE728 KDR c.3718G>C p.E1240Q EX28 4.1% missensePE668 EGFR c.2236_2250delGAATTAAGAGAAGCAp.E746_A750del EX19 32.7% cds-delPE717 ARID1A c.2632C>G p.Q878E EX8 4.0% missensePE754 EMID2 c.224T>A p.V75E EX2 1.2% missensePE565 EGFR c.2573T>G p.L858R EX21 3.5% missensePE770 EGFR c.2217_2234dupAATTCCCGTCGCTATCAAp.I740_K745dup EX19 12.5% cds-insPE824 TP53 c.572C>G p.P191R EX6 0.5% missensePE714 NF1 c.1078C>G p.P360A EX10 2.3% missensePE724 MAF c.398G>A p.R133H EX1E 4.3% missensePE853 GAB2 c.1435A>G p.M479V EX6 4.0% missensePE656 ESR1 c.1088G>A p.R363K EX5 0.6% missensePE891 FLT1 c.1523G>A p.R508H EX11 3.2% missensePE895 HNF1A c.845C>G p.A282G EX4 1.2% missensePE935 FLT3 c.22G>C p.G8R EX1 13.4% missensePE223 DNMT3A c.919C>A p.P307T EX8 2.1% missensePE222 TP53 c.1024C>T p.R342* EX10 1.8% nonsensePE251 TP53 c.1024C>T p.R342* EX10 0.7% nonsensePE352 RB1 c.1395_1411delAGAACGATTATCCATTCinsCGp.E465_Q471delinsDEEX15 85.0% cds-indelPE356 ATM c.6805C>T p.Q2269* EX46 1.1% nonsensePE394 LRP1B c.12920C>G p.P4307R EX84 33.0% missense

PE539 ROS1 c.4355T>C p.V1452A EX27 2.2% missensePE511 TP53 c.863_872delATCTCCGCAAp.N288Rfs*54 EX8 22.0% frameshiftPE728 UGT1A1 c.570C>G p.F190L EX1 1.4% missensePE731 CDC73 c.265C>T p.P89S EX3 4.0% missensePE495 GRIN2A c.4151C>T p.S1384L EX13E 2.8% missensePE565 MSH3 c.238-1G>A . IVS1 1.1% splice-3PE714 TMEM132D c.670G>A p.V224M EX2 7.2% missensePE915 BCL2A1 c.300C>G p.I100M EX1 16.6% missensePE891 ABL1 c.343T>C p.Y115H EX3 2.7% missensePE935 EPHB1 c.2609G>T p.R870L EX14 8.9% missensePE252 DNMT3A c.919C>A p.P307T EX8 1.7% missensePE270 AKAP9 c.1168T>C p.S390P EX8 17.6% missensePE264 MNDA c.571-1G>T . IVS4 43.0% splice-3PE351 MLL3 c.11009A>G p.N3670S EX43 6.5% missensePE378 ATR c.5314A>C p.T1772P EX31 1.5% missensePE344 FANCM c.5987C>T p.S1996F EX22 4.9% missensePE386 NF2 c.1564_1567delGAGAp.E522Kfs*27 EX14 11.5% frameshiftPE364 TP53 c.586C>T p.R196* EX6 63.0% nonsensePE418 ROBO3 c.609C>A p.D203E EX3 3.0% missensePE503 ZFHX3 c.7995T>A p.N2665K EX9 0.6% missensePE506 MLL c.1455T>G p.D485E EX3 2.9% missensePE517 EGFR c.2369C>T p.T790M EX20 1.4% missensePE411 EGFR c.2573T>G p.L858R EX21 87.1% missensePE636 CTNNB1 c.121A>G p.T41A EX3 1.3% missensePE654 LRP1B c.2957A>T p.H986L EX19 6.6% missensePE660 SMAD4 c.1015T[3>2] p.F339Lfs*45 EX9 1.3% frameshiftPE705 TP53 c.818G>A p.R273H EX8 57.9% missensePE680 EPHB2 c.784G>A p.A262T EX3 15.5% missensePE757 CDK12 c.3083T>A p.M1028K EX11 49.2% missensePE787 INPP4B c.1822C>G p.Q608E EX19 1.9% missensePE724 EGFR c.2389T>A p.C797S EX20 4.5% missensePE753 CDK12 c.103C>A p.R35S EX1 19.9% missensePE592 ATR c.7213G>T p.E2405* EX43 5.7% nonsensePE919 CTCF c.298G>T p.V100F EX3 1.2% missensePE296 SLIT2 c.1258C>T p.R420W EX13 1.2% missensePE311 EPHA5 c.538G>A p.D180N EX3 0.5% missensePE341 PTPRF c.5653G>C p.E1885Q EX33 2.2% missensePE400 MLL3 c.1387C>T p.Q463* EX10 13.4% nonsensePE364 CASP8 c.987G>C p.L329F EX8 22.0% missensePE457 OGDH c.838C>G p.L280V EX7 1.9% missensePE576 TOP2A c.278C>T p.A93V EX4 11.8% missensePE588 DICER1 c.3860C>G p.S1287* EX22 4.1% nonsensePE553 MLL3 c.13903G>C p.D4635H EX53 35.5% missensePE613 TP53 c.882_885delGCCTp.E294Dfs*50 EX8 27.2% frameshiftPE538 EGFR c.2236_2248delinsCAACp.E746_A750delinsQPEX19 29.7% cds-indelPE678 RPS6KB1 c.1394C>T p.T465I EX15E 6.6% missensePE873 TP53 c.493C>T p.Q165* EX5 0.4% nonsensePE877 MLL3 c.4924G>C p.E1642Q EX33 2.0% missensePE911 ERBB2 c.2326delGinsTTGTp.G776delinsLC EX20 3.7% cds-indelPE821 KEAP1 c.1408C>T p.R470C EX4 3.2% missensePE632 TP53 c.461G>T p.G154V EX5 1.3% missensePE903 MLL c.6851A>T p.D2284V EX27 2.9% missensePE219 ASXL1 c.2790G>A p.W930* EX13 0.7% nonsensePE215 NOTCH3 c.5063C>T p.S1688F EX27 8.2% missense

PE424 JAK1 c.881C>T p.S294L EX7 1.0% missensePE461 TP53 c.422G>A p.C141Y EX5 81.3% missensePE417 CDKN1B c.80C>T p.S27L EX1 56.7% missensePE473 FAT3 c.12667C>A p.Q4223K EX23 7.8% missensePE397 NOTCH2 c.6536C>T p.S2179L EX34E 3.3% missensePE531 RAD51B c.1138A>G p.T380A EX11E 5.8% missensePE587 LRP1B c.1991G>T p.W664L EX13 5.9% missensePE665 SETD2 c.7093C>T p.Q2365* EX16 1.1% nonsensePE572 PTEN c.151G>T p.D51Y EX2 16.9% missensePE704 SF3B1 c.2221A>G p.K741E EX15 23.7% missensePE599 OR5L2 c.127C>A p.L43M EX1E 26.9% missensePE690 PDGFRA c.1757G>A p.W586* EX12 23.9% nonsensePE622 PTPRD c.18GCT[5>4] p.L7[6>5] EX12 0.6% cds-delPE781 EGFR c.2236_2250delGAATTAAGAGAAGCAp.E746_A750del EX19 9.7% cds-delPE721 PLXNB2 c.4301A>G p.K1434R EX27 0.5% missensePE697 TP53 c.398T>C p.M133T EX5 47.9% missensePE858 SUFU c.205G>C p.D69H EX2 15.5% missensePE884 MLL2 c.16342C>T p.R5448* EX52 53.4% nonsensePE913 LRP1B c.12589C>A p.P4197T EX82 25.8% missensePE231 NF1 c.6371G>T p.G2124V EX42 28.5% missensePE274 CUL3 c.2059G>A p.E687K EX15 9.3% missensePE252 JAK1 c.2543T>A p.L848H EX18 42.3% missensePE400 RBM10 c.865G>A p.V289I EX8 4.8% missensePE410 EGFR c.2573T>G p.L858R EX21 63.7% missensePE395 LRP1B c.3733C>G p.L1245V EX23 23.1% missensePE457 TUBA3C c.437G>C p.G146A EX4 2.5% missensePE396 BRCA2 c.4484T>G p.V1495G EX11 1.9% missensePES121 EGFR c.2573T>G p.L858R EX21 2.9% missensePE550 LRP1B c.3769C>T p.P1257S EX24 1.6% missensePE551 DNMT3A c.919C>T p.P307S EX8 1.0% missensePE701 TP53 c.412G>C p.A138P EX5 76.3% missensePE669 EGFR c.2369C>T p.T790M EX20 0.4% missensePE689 CNOT4 c.1933G>T p.D645Y EX12E 1.5% missensePE740 MYH9 c.1588G>A p.E530K EX14 2.1% missensePE667 TP53 c.673-2A>G . IVS6 3.1% splice-3PE793 PIK3CG c.317G[2>1] p.W106Cfs*52 EX2 27.8% frameshiftPE911 PPP2R1A c.547C>T p.R183W EX5 1.7% missensePE885 FANCM c.1525G>C p.V509L EX9 10.6% missensePE822 TP53 c.527G>A p.C176Y EX5 48.6% missensePE870 EGFR c.2236_2250delGAATTAAGAGAAGCAp.E746_A750del EX19 38.3% cds-delPE252 PDK1 c.355C>T p.Q119* EX3 3.4% nonsensePE270 OR2T4 c.932C>A p.T311N EX1 15.9% missensePE264 EGFR c.1939G>A p.A647T EX17 39.6% missensePE344 PIK3R2 c.500C>T p.T167M EX5 26.7% missensePE477 FGFR3 c.1882G>A p.D628N EX14 20.0% missensePE418 BCL2A1 c.227A[4>3] p.K77Rfs*14 EX1 0.6% frameshiftPE506 TP53 c.418_426dupACCTGCCCTp.T140_P142dup EX5 49.3% cds-insPE517 TP53 c.1024C>T p.R342* EX10 2.4% nonsensePE654 LRP1B c.2628G>T p.E876D EX16 16.1% missensePE628 EGFR c.2573T>G p.L858R EX21 53.0% missensePE689 FLT1 c.2827G>C p.E943Q EX21 1.4% missensePE384 CIC c.3565G>A p.V1189M EX15 0.5% missensePE787 EGFR c.2369C>T p.T790M EX20 2.9% missensePE840 EGFR c.2573T>G p.L858R EX21 1.7% missense

PE724 SBDS c.318G>C p.Q106H EX3 10.4% missensePE751 CBL c.1244G>T p.G415V EX9 1.0% missensePE900 FANCA c.897C>G p.F299L EX11 2.8% missensePE869 ATR c.3079G>A p.E1027K EX15 2.8% missensePE816 TP53 c.949C>T p.Q317* EX9 11.1% nonsensePE292 DDR2 c.1165C>T p.P389S EX11 1.6% missensePE356 FGFR3 c.995A>T p.E332V EX8 51.3% missensePE346 SMAD2 c.930G>T p.R310S EX8 0.6% missensePE462 BRAF c.1330C>T p.R444W EX11 1.3% missensePE448 TP53 c.997_1003delCGTGGGCp.R333Vfs*10 EX10 19.3% frameshiftPE500 ARID1A c.740C>A p.A247D EX1 3.7% missensePE539 MTOR c.3843_3844delGGinsTTp.E1282* EX26 15.9% stop-gainPE594 CBL c.1246T>C p.C416R EX9 0.9% missensePE672 DICER1 c.1218G>T p.Q406H EX9 1.0% missensePE635 ALK c.3604G>A p.G1202R EX23 6.7% missensePE484 EIF3A c.2362C>T p.H788Y EX16 10.7% missensePE736 CCND3 c.67G>A p.G23R EX1 0.9% missensePE730 TGFBR2 c.1405C>T p.Q469* EX6 3.2% nonsensePE705 CYP2C8 c.199G>A p.V67M EX2 0.7% missensePE680 SMARCA4 c.4805A>G p.K1602R EX34 15.6% missensePE495 HCLS1 c.1028C>A p.A343D EX12 3.3% missensePE804 CDK13 c.3641C>T p.A1214V EX13 1.5% missensePE657 TP53 c.578A>T p.H193L EX6 6.8% missensePE714 EGFR c.2369C>T p.T790M EX20 4.7% missensePE867 RBM10 c.943G[2>1] p.G315Afs*16 EX9 60.9% frameshiftPE915 NOTCH4 c.5998G>C p.E2000Q EX30E 8.8% missensePE891 FLT4 c.2168G>A p.G723E EX15 3.9% missensePE223 FGFR1 c.1363C>T p.R455C EX10 8.4% missensePE297 XRCC2 c.58G>T p.G20C EX2 6.6% missensePE309 PTPRD c.5473G>C p.G1825R EX44 28.3% missensePE207 RB1 c.1954A[6>7] p.V654Sfs*14 EX19 19.3% frameshiftPE348 BAP1 c.660-1G>C . IVS8 10.4% splice-3PE356 EP300 c.6188C>T p.S2063F EX31 15.6% missensePE546 WT1 c.986C>A p.T329K EX5 1.6% missensePE593 MYCN c.1073C>T p.P358L EX3E 4.9% missensePE590 EXT1 c.56T>C p.L19P EX1 0.5% missensePE654 NCOR1 c.28C>G p.Q10E EX2 70.3% missensePE670 EGFR c.2572_2573delCTinsAGp.L858R EX21 4.1% missensePE385 CHD4 c.3724G>A p.E1242K EX25 3.6% missensePE649 RBM10 c.198G[2>1] p.E67Sfs*132 EX2 14.6% frameshiftPE788 NOTCH1 c.6905C[2>1] p.S2303Vfs*2 EX34E 3.7% frameshiftPE679 NRAS c.364A>G p.T122A EX4 8.5% missensePE831 TP53 c.701A>G p.Y234C EX7 14.2% missensePE840 FCGR3A c.203C>G p.S68* EX4 2.0% nonsensePE792 SETD2 c.1198C>T p.R400* EX3 3.6% nonsensePE800 MLL3 c.1142C>A p.A381E EX8 1.2% missensePE724 PTPRT c.3052C>T p.R1018* EX23 4.4% nonsensePE863 EGFR c.2573T>G p.L858R EX21 39.2% missensePE854 GPX1 c.475G>A p.D159N EX2E 2.2% missensePE250 LRP1B c.4048_4049insG p.Y1350*fs*1 EX25 1.9% frameshiftPE297 EGFR c.2369C>T p.T790M EX20 17.2% missensePE207 NOTCH1 c.3048C>A p.S1016R EX19 20.3% missensePE382 DICER1 c.4586A>T p.N1529I EX24 3.8% missensePE399 GRIN2A c.397A>G p.M133V EX2 5.6% missense

PE394 MAP2K4 c.643G>A p.A215T EX6 22.2% missensePE503 MTOR c.7393G>A p.E2465K EX55 1.0% missensePE546 ATR c.2742_2743delGGinsTTp.V915F EX13 1.1% missensePE407 CTCF c.1024C>T p.R342C EX5 0.5% missensePE318 RB1 c.25_26insG p.T9Sfs*22 EX1 47.6% frameshiftPE593 CBR1 c.245G>C p.G82A EX1 9.9% missensePE728 KDM5A c.4571C>A p.S1524Y EX27 4.0% missensePE709 TP53 c.1009C>T p.R337C EX10 15.8% missensePE385 PMS2 c.-87-u195G>A . . 1.1% promoterPE649 EGFR c.2573T>G p.L858R EX21 20.6% missensePE745 IL6 c.38C>T p.A13V EX2 9.5% missensePE565 ASXL1 c.1900_1922delAGAGAGGCGGCCACCACTGCCATp.E635Rfs*15 EX13E 0.5% frameshiftPE795 MED12 c.6163C>T p.Q2055* EX42 11.1% nonsensePE800 LRP1B c.10784C>A p.S3595* EX70 10.0% nonsensePE876 APC c.8204G>A p.G2735E EX16E 2.2% missensePE899 ARID1B c.986C>G p.A329G EX1 12.6% missensePE220 TP53 c.701A>G p.Y234C EX7 23.7% missensePE325 CDKN2A c.322G>T p.D108Y EX2 37.7% missensePE336 PIK3CA c.1573G>A p.E525K EX10 20.6% missensePE208 TP53 c.743G>A p.R248Q EX7 0.6% missensePE363 RUNX1 c.559G>A p.A187T EX6 20.1% missensePE403 ARID1A c.5161C>T p.R1721* EX20E 1.4% nonsensePE461 EPHA3 c.300G>T p.K100N EX3 3.4% missensePE417 EPHA5 c.920C>G p.P307R EX4 36.8% missensePE524 ACVR1B c.818_819delGCinsTTp.C273F EX5 7.1% missensePE601 EGFR c.2235_2249delGGAATTAAGAGAAGCp.E746_A750del EX19 6.6% cds-delPE528 EGFR c.2573T>G p.L858R EX21 6.0% missensePE594 TCF3 c.1564A>C p.K522Q EX16 25.0% missensePE672 EGFR c.2235_2249delGGAATTAAGAGAAGCp.E746_A750del EX19 5.7% cds-delPE614 TAF15 c.1380_1403delCGGCGGCTATGGTGGGGACAGAGGp.G454_G461[4>3]EX15 27.9% cds-delPE783 TP53 c.761TCA[2>1] p.I254[2>1] EX7 25.0% cds-delPE662 TP53 c.358A>T p.K120* EX4 2.6% nonsensePE774 AKAP9 c.1169C>T p.S390F EX8 4.2% missensePE874 DNMT3A c.2323-2A>C . IVS19 1.8% splice-3PE882 ERCC3 c.1624A>G p.R542G EX10 0.5% missensePE828 NF1 c.374G>C p.R125P EX4 38.1% missensePE219 CIITA c.1564C>T p.P522S EX11 2.1% missensePE215 TP53 c.809T>G p.F270C EX8 20.1% missensePE403 AR c.1390G[2>1] p.G464Afs*15 EX1 5.0% frameshiftPE429 TP53 c.1023C[2>1] p.R342Efs*3 EX10 35.3% frameshiftPE417 ROBO3 c.4103G>T p.S1368I EX27 54.1% missensePE548 MLL2 c.12100_12101insGAAGTp.S4034*fs*1 EX39 2.2% frameshiftPE531 BRCA2 c.8941G>A p.E2981K EX22 3.6% missensePE499 TP53 c.559+2T>C . IVS5 17.7% splice-5PE511 PIK3CA c.1252G>A p.E418K EX8 6.1% missensePE639 MAP2K1 c.167A>C p.Q56P EX2 4.1% missensePE672 U2AF1 c.566G>A p.R189H EX7 1.6% missensePE508 FGFR4 c.2321C[2>1] p.C775Afs*102 EX18E 1.8% frameshiftPE572 CDH23 c.7495G>T p.V2499L EX54 52.1% missensePE702 TP53 c.764T>A p.I255N EX7 24.3% missensePE622 FLT4 c.1610C>T p.S537F EX12 2.0% missensePE781 FOXL2 c.492G[4>5] p.L166Afs*73 EX1E 17.5% frameshiftPE814 GALNT12 c.1021G>A p.E341K EX5 1.3% missensePE697 NTRK3 c.772C>G p.L258V EX9 2.2% missense

PE749 CYP2D6 c.482G>C p.C161S EX3 2.5% missensePE774 PALB2 c.1523C>T p.P508L EX4 3.5% missensePE858 ARID1B c.2905C>T p.Q969* EX10 14.7% nonsensePE798 EGFR c.2125G>A p.E709K EX18 1.7% missensePE884 PTPRD c.1816C>A p.Q606K EX23 56.3% missensePE922 TP53 c.583A>T p.I195F EX6 47.5% missensePE913 FAM5C c.1268C>G p.T423R EX8E 7.6% missensePES130 MLL4 c.1096G>T p.E366* EX3 0.6% nonsensePE308 TP53 c.811G>C p.E271Q EX8 0.8% missensePE216 BRCA2 c.868G>T p.V290F EX10 4.3% missensePE303 ABCC11 c.342G>C p.E114D EX4 4.2% missensePE301 FAT1 c.8662G>A p.V2888I EX10 1.7% missensePE207 PDCD1LG2 c.553C[5>4] p.P186Lfs*14 EX4 13.4% frameshiftPE370 TP53 c.319delT p.Y107Tfs*16 EX4 20.3% frameshiftPE413 EZH2 c.1585G>A p.D529N EX14 17.8% missensePE594 NRAS c.35G>T p.G12V EX2 20.3% missensePE561 FAT2 c.2113T>A p.Y705N EX1 29.9% missensePE856 RB1 c.1413_1421+17delAAATTTTAGGTAAATTTTTTACTTTT. EX15-IVS1541.4% spanPE922 APC c.7288G>C p.D2430H EX16E 19.3% missensePE270 TP53 c.839_857delGAGACCGGCGCACAGAGGAp.R280Kfs*59 EX8 5.8% frameshiftPE223 MTOR c.3365T>G p.F1122C EX22 0.5% missensePE207 TP53 c.743G>A p.R248Q EX7 27.5% missensePE356 NF1 c.3058G>C p.E1020Q EX23 22.7% missensePE394 JAK2 c.1156G>A p.E386K EX9 16.9% missensePE447 TP53 c.710T>A p.M237K EX7 15.5% missensePE446 EGFR c.2240_2254delTAAGAGAAGCAACATp.L747_T751del EX19 10.6% cds-delPE500 PIK3C2G c.4185+1G>A . IVS31 9.2% splice-5PE542 XPO1 c.306A[7>8] p.Y105Ifs*12 EX5 4.1% frameshiftPE539 SMARCA4 c.935C[5>6] p.A314Rfs*73 EX6 32.4% frameshiftPE584 EGFR c.2170G>A p.G724S EX18 7.0% missensePE511 TOP1 c.418C>T p.R140* EX6 3.5% nonsensePE593 FAT2 c.8368G>A p.V2790I EX9 16.8% missensePE713 IKZF1 c.184C>G p.Q62E EX4 15.7% missensePE767 EGFR c.2573T>G p.L858R EX21 3.6% missensePE717 EGFR c.2573T>G p.L858R EX21 4.7% missensePE770 ASXL1 c.626C>T p.S209F EX8 2.2% missensePE794 POU2AF1 c.443T>C p.I148T EX4 23.9% missensePE469 FRMPD4 c.3271C>G p.Q1091E EX16 29.0% missensePE838 TP53 c.659A>G p.Y220C EX6 1.1% missensePE891 TP53 c.730G>T p.G244C EX7 3.4% missensePE299 ERBB2 c.916A>T p.T306S EX8 4.2% missensePE315 TET2 c.3646C>T p.R1216* EX6 0.7% nonsensePE207 ARID2 c.2256A>C p.Q752H EX15 10.1% missensePE317 PIK3C2B c.1347_1353delCTGCCGCp.Y449*fs*1 EX7 13.2% frameshiftPE459 SMAD4 c.1333C>T p.R445* EX11 9.4% nonsensePE543 ZMAT3 c.526C>G p.R176G EX4 3.4% missensePE518 TERT c.-58-u6G>A . . 4.7% promoterPE584 MAP2K1 c.807G>T p.K269N EX7 5.2% missensePE594 RICTOR c.11T>C p.I4T EX1 19.1% missensePE732 EP300 c.5158C>T p.Q1720* EX31E 2.7% nonsensePE784 RPS6KB1 c.1459A>G p.T487A EX15E 21.9% missensePE744 ERBB2 c.2313_2324dupATACGTGATGGCp.Y772_A775dup EX20 12.3% cds-insPE679 IKZF1 c.317G>T p.G106V EX4 11.0% missensePE812 RPTOR c.1946C>G p.A649G EX17 16.3% missense

PE778 MLL3 c.14689A>T p.I4897F EX59E 1.0% missensePE853 TP53 c.685dupT p.C229Lfs*11 EX7 4.9% frameshiftPE790 CDK4 c.70C>T p.R24C EX2 12.0% missensePE653 KDM5A c.1643A>T p.H548L EX12 48.2% missensePE901 SRSF2 c.182G>A p.R61H EX1 11.9% missensePE928 FAT2 c.9754G>A p.G3252S EX13 1.2% missensePE869 RB1 c.2493C>G p.I831M EX24 2.2% missensePE906 TP53 c.211C[6>7] p.V73Rfs*76 EX4 28.5% frameshiftPE914 USH2A c.4664G>C p.G1555A EX22 6.5% missensePE655 ARID1A c.3985C>T p.Q1329* EX16 31.1% nonsensePE270 CCT5 c.1003A>G p.I335V EX8 29.3% missensePE274 EGFR c.2389T>A p.C797S EX20 6.4% missensePE252 PABPC1 c.394T>G p.C132G EX3 17.6% missensePE364 FLT3 c.760C>T p.Q254* EX7 2.9% nonsensePE444 FLT4 c.2281G>A p.A761T EX15 1.0% missensePE418 PIK3CG c.850G>A p.E284K EX2 8.7% missensePE463 TP53 c.451C>A p.P151T EX5 27.8% missensePE457 RBM10 c.1316G>T p.G439V EX11 1.5% missensePE506 ARID1A c.229G>T p.A77S EX1 32.9% missensePE581 STAG2 c.3658G>C p.E1220Q EX33 94.4% missensePE654 LRP1B c.12142G>T p.D4048Y EX79 35.6% missensePE630 TP53 c.839G>T p.R280I EX8 74.0% missensePE733 EGFR c.2236_2250delGAATTAAGAGAAGCAp.E746_A750del EX19 27.3% cds-delPE669 TP53 c.860_886delAGAATCTCCGCAAGAAAGGGGAGCCTCinsGp.E287Gfs*10 EX8 4.1% frameshiftPE689 MLL c.699C>G p.I233M EX3 1.6% missensePE479 VHL c.556G>A p.E186K EX3E 19.8% missensePE757 TP73 c.1695C>A p.N565K EX14E 52.1% missensePE768 EGFR c.2369C>T p.T790M EX20 2.3% missensePE753 NAP1L1 c.472dupG p.D158Gfs*2 EX7 9.9% frameshiftPE739 GPS2 c.670C>T p.Q224* EX8 28.5% nonsensePE892 ARID2 c.3085C>T p.Q1029* EX15 9.5% nonsensePE893 RB1 c.706A[3>2] p.E237Nfs*27 EX7 62.9% frameshiftPE869 LRP1B c.3522dupT p.E1175*fs*1 EX23 3.2% frameshiftPE925 EPPK1 c.7126C>G p.L2376V EX1E 8.8% missensePE222 NFE2L2 c.85G>C p.D29H EX2 3.0% missensePE251 ARID1A c.5047G>A p.E1683K EX19 0.8% missensePE378 ACTG1 c.-6-2A>G . IVS1 3.4% splice-3PE346 RBM10 c.883C>T p.R295* EX8 9.7% nonsensePE500 DDR2 c.994C>T p.R332W EX9 20.4% missensePE527 INPP4B c.890G>T p.R297L EX13 29.3% missensePE539 LRP1B c.7688G>T p.R2563L EX47 8.7% missensePE673 EGFR c.865_866delGCinsTAp.A289Y EX7 72.7% missensePE728 LRP1B c.10292G>T p.C3431F EX65 2.3% missensePE610 EGFR c.2369C>T p.T790M EX20 12.2% missensePE730 ZMAT3 c.394G>C p.G132R EX4 2.6% missensePE705 CDK8 c.1241G>C p.G414A EX12 0.9% missensePE804 CDH23 c.3802G>A p.V1268M EX32 9.6% missensePE714 MLL3 c.100A>T p.K34* EX1 2.6% nonsensePE742 OR6F1 c.739G>A p.V247M EX1E 31.9% missensePE915 EGFR c.2573T>G p.L858R EX21 42.4% missensePE891 MLL3 c.14176C>T p.P4726S EX55 2.7% missensePE284 EGFR c.2240_2257delTAAGAGAAGCAACATCTCp.L747_P753delinsSEX19 2.4% cds-indelPE305 BRIP1 c.2894G>A p.R965K EX19 1.0% missensePE248 SLIT1 c.1915G>A p.V639I EX19 1.6% missense

PE534 PTCH2 c.988G>T p.E330* EX8 4.0% nonsensePE523 EPHA3 c.2941G>T p.V981F EX17E 27.5% missensePE638 ATRX c.2158A>G p.K720E EX9 3.4% missensePE737 ZFP36L1 c.657C>A p.H219Q EX3E 88.7% missensePE700 MLL3 c.11887G>T p.A3963S EX46 0.5% missensePE650 EGFR c.2369C>T p.T790M EX20 32.5% missensePE748 CYP2C8 c.782G>C p.R261P EX5 7.0% missensePE900 ROS1 c.4738G>A p.E1580K EX29 1.1% missensePE872 AXIN2 c.118G>T p.V40L EX2 28.0% missensePE833 ARID1B c.6681A>G p.I2227M EX20E 33.5% missensePE256 AR c.1199C>T p.A400V EX1 0.4% missensePE218 DNMT3A c.2708C>A p.A903D EX23 1.1% missensePE319 EGFR c.2235_2249delGGAATTAAGAGAAGCp.E746_A750del EX19 56.0% cds-delPE400 LRP1B c.11137T>A p.F3713I EX73 7.5% missensePE391 EGFR c.2239_2256delTTAAGAGAAGCAACATCTp.L747_S752del EX19 15.5% cds-delPE429 RANBP2 c.2083G>C p.E695Q EX15 2.2% missensePE445 MLL3 c.4963C>A p.L1655I EX33 0.7% missensePE457 NCSTN c.1937C>G p.S646C EX16 3.4% missensePE523 AR c.1175_1189delCGCTGGACTACGGCAp.P392_S397delinsREX1 20.4% cds-indelPES121 TP53 c.503A>C p.H168P EX5 49.8% missensePE574 EGFR c.2573T>G p.L858R EX21 6.1% missensePE515 SDHA c.1776T>A p.H592Q EX13 2.3% missensePE588 ARID1B c.463G>C p.E155Q EX1 1.2% missensePE737 EWSR1 c.1904G>A p.G635E EX17 1.2% missensePE538 TENM3 c.5762A>C p.N1921T EX25 4.3% missensePE689 FANCD2 c.550G>C p.D184H EX8 1.4% missensePE698 MDM4 c.1323G>T p.E441D EX11E 2.1% missensePE650 SMAD4 c.1529G>T p.G510V EX12E 57.2% missensePE678 TERT c.-58-u66C>T . . 5.6% promoterPE877 TET2 c.895G>T p.D299Y EX3 2.8% missensePE591 SMARCA4 c.1441C>T p.Q481* EX9 47.3% nonsensePE821 BCL2L11 c.304G>A p.D102N EX2 4.7% missensePE632 SOX2 c.311A>C p.E104A EX1E 3.8% missensePE343 ZIC3 c.896G>T p.W299L EX1 8.4% missensePE436 TP53 c.734G>A p.G245D EX7 4.1% missensePE472 ATM c.7037C>T p.A2346V EX48 2.9% missensePE468 TBX3 c.1817C>T p.T606M EX8E 1.8% missensePE581 KDM5A c.2619A>T p.K873N EX19 65.0% missensePE638 HIST1H1C c.525G>C p.K175N EX1E 1.3% missensePE587 PTEN c.94ATT[2>1] p.I32[2>1] EX2 1.3% cds-delPE572 STK11 c.632G[2>1] p.T212Pfs*75 EX5 53.4% frameshiftPE758 SMARCB1 c.91G>C p.E31Q EX1 3.6% missensePE858 APOBEC3B c.901A>T p.T301S EX6 9.1% missensePE840 ROBO3 c.338C>T p.A113V EX2 5.1% missensePE938 MLL2 c.14747C>T p.P4916L EX48 0.8% missensePE890 PTPRT c.1004C>A p.A335E EX7 7.3% missensePE913 PARK2 c.524C>T p.T175I EX4 23.4% missensePE917 RNF43 c.224_231delTAACTCCAinsCp.I75Tfs*6 EX2 14.8% frameshiftPE436 NF2 c.364-3_364-1delCAGinsAAA. IVS3 2.7% splice-3PE441 TP53 c.726_727delCAinsTp.M243Wfs*4 EX7 11.8% frameshiftPES121 STAT3 c.1265delG p.G422Afs*9 EX14 0.2% frameshiftPE520 ARID1B c.980G>C p.G327A EX1 16.7% missensePE638 EGFR c.2573T>G p.L858R EX21 23.8% missensePE586 MED12 c.3128T>G p.L1043R EX22 88.5% missense

PE587 KDM5A c.5025C>G p.F1675L EX28E 1.8% missensePE741 TP53 c.857A>T p.E286V EX8 7.7% missensePE816 JAK2 c.1849G>T p.V617F EX14 0.7% missensePE913 ARID2 c.4831C>T p.Q1611* EX16 22.4% nonsensePE930 TP53 c.406C>G p.Q136E EX5 2.6% missensePE255 RBM10 c.777_780delCCTCp.L260Tfs*70 EX7 1.2% frameshiftPE252 KRAS c.35G>A p.G12D EX2 51.0% missensePE341 SLX4 c.1894G>C p.G632R EX8 4.5% missensePE400 SMARCA4 c.1944-2A>G . IVS12 6.8% splice-3PE424 C15orf23 c.133G>A p.G45S EX1 1.7% missensePE364 EGFR c.1881-1G>A . IVS15 1.1% splice-3PE457 OR5L2 c.86T>A p.L29Q EX1E 6.0% missensePE396 CTNNB1 c.95A>T p.D32V EX3 1.0% missensePE545 EGFR c.2573T>G p.L858R EX21 2.7% missensePE588 ARHGAP35 c.3977C>G p.S1326* EX4 4.8% nonsensePE440 SRC c.1267C>A p.Q423K EX12 6.8% missensePE660 FH c.128G>T p.R43L EX1 1.4% missensePE669 APC c.5846A[4>3] p.K1950Sfs*20 EX16E 9.2% frameshiftPE763 FAS c.676+1G>T . IVS8 8.6% splice-5PE689 ESR1 c.1135C>G p.L379V EX6 1.0% missensePE729 PTPRD c.18GCT[5>4] p.L7[6>5] EX12 0.5% cds-delPE698 MLH3 c.3280+1G>T . IVS2 29.1% splice-5PE793 EPHA3 c.1849G>T p.D617Y EX10 16.6% missensePE563 KEAP1 c.1288G>A p.G430S EX3 71.4% missensePE883 PTCH2 c.247G>T p.E83* EX2 1.2% nonsensePE753 JAK1 c.2689G>A p.E897K EX20 0.5% missensePE877 ACIN1 c.1102G>C p.E368Q EX6 3.3% missensePE885 MEN1 c.1742C>T p.A581V EX10E 22.4% missensePE822 NPM1 c.407T>G p.L136* EX5 16.9% nonsensePE935 SMAD3 c.202C>A p.P68T EX1 18.8% missensePE252 EGFR c.2236_2250delGAATTAAGAGAAGCAp.E746_A750del EX19 3.1% cds-delPE334 ARID1B c.4723C>G p.L1575V EX18 12.4% missensePE264 FANCC c.371C>G p.A124G EX5 5.5% missensePE335 EGFR c.2156G>C p.G719A EX18 7.8% missensePE364 PIK3R2 c.2186G>A p.(*729=) EX16E 1.1% stop-retainedPE448 NOTCH1 c.7532C>T p.T2511I EX34E 1.3% missensePE477 EGFR c.2573T>G p.L858R EX21 23.1% missensePE411 BRCA2 c.5831A>T p.K1944I EX11 13.4% missensePE628 CDK12 c.3377_3378delAAinsCCp.Q1126P EX13 1.8% missensePE660 MED12 c.6044G>T p.R2015M EX41 5.7% missensePE682 SF3B1 c.2221A>G p.K741E EX15 9.7% missensePE787 ABCC11 c.2360C>T p.S787F EX18 1.1% missensePE840 TP53 c.800_802delGGA p.R267_N268delinsHEX8 0.5% cds-indelPE759 MLL2 c.8596G>A p.E2866K EX34 1.1% missensePE725 EGFR c.2573T>G p.L858R EX21 84.5% missensePE888 EGFR c.2573T>G p.L858R EX21 21.2% missensePE886 MET c.2914G>C p.D972H EX14 10.7% missensePE735 CBL c.650C>A p.S217Y EX4 3.3% missensePE863 JUN c.334G>A p.E112K EX1E 2.3% missensePE592 FANCG c.1666C>T p.Q556* EX13 6.6% nonsensePE816 ERBB2 c.2313_2324dupATACGTGATGGCp.Y772_A775dup EX20 9.0% cds-insPE918 SDHC c.106G>T p.E36* EX3 1.5% nonsensePE250 TP53 c.524G>A p.R175H EX5 0.5% missensePE297 NTRK1 c.1252G>A p.V418I EX11 16.7% missense

PE207 ARID1A c.666C[6>5] p.P224Rfs*8 EX1 20.2% frameshiftPE382 TP53 c.395A>G p.K132R EX5 6.6% missensePE393 TP53 c.473G>C p.R158P EX5 6.1% missensePE503 SETD2 c.1447C>G p.L483V EX3 0.8% missensePE486 EGFR c.2573T>G p.L858R EX21 27.7% missensePE546 IMPG1 c.127G>A p.E43K EX2 1.7% missensePE407 MAPK1 c.576G>C p.W192C EX4 0.7% missensePE442 NCOR2 c.2160G>C p.E720D EX20 8.1% missensePE318 CTCF c.517_518delGGinsATp.G173I EX3 25.5% missensePE593 EPHA7 c.2545A>G p.I849V EX15 9.8% missensePE654 PALB2 c.662T>G p.V221G EX4 72.8% missensePE535 CDKN2A c.43TGGC[2>1] p.L16Pfs*9 EX1 2.5% frameshiftPE728 H3F3C c.316G>C p.D106H EX1E 3.5% missensePE659 ARID1A c.5938C>A p.R1980S EX20E 0.5% missensePE649 MLL3 c.5082+2T>A . IVS34 1.2% splice-5PE565 ASXL1 c.2464dupA p.T822Nfs*11 EX13E 1.1% frameshiftPE564 PMS2 c.411T>G p.F137L EX5 37.2% missensePE800 PIK3CA c.1193G>T p.R398L EX7 6.4% missensePE724 CEBPA c.382C>G p.P128A EX1E 20.2% missensePE879 FANCM c.4956G>T p.M1652I EX20 0.6% missensePE876 HIST1H3F c.355A>G p.T119A EX1E 0.8% missensePE899 EGFR c.2235_2249delGGAATTAAGAGAAGCp.E746_A750del EX19 3.5% cds-delPES130 JAK1 c.1979A>G p.D660G EX14 10.1% missensePE222 TP53 c.1005dupT p.E336*fs*1 EX10 17.3% frameshiftPE222 MED12 c.462G>A p.M154I EX4 0.9% missensePE260 SF3B1 c.2098A>G p.K700E EX15 2.4% missensePE207 EP300 c.2939C>T p.A980V EX15 8.8% missensePE339 FAT2 c.6995C>G p.S2332C EX9 2.2% missensePE320 LRP1B c.12340+2T>A . IVS80 20.0% splice-5PE370 EGFR c.2573T>G p.L858R EX21 55.4% missensePE492 RAD50 c.2799A>C p.K933N EX17 3.9% missensePE594 ARID1B c.1627A>G p.S543G EX2 21.3% missensePE777 EPHA5 c.1040C>G p.S347C EX4 7.5% missensePE691 CARD11 c.1100C>T p.T367M EX8 0.5% missensePE695 TP53 c.520A>T p.R174W EX5 6.3% missensePE832 C11orf30 c.851C>T p.S284L EX8 1.4% missensePE710 FLT4 c.2872G>A p.G958R EX21 4.4% missensePE715 MAP2K4 c.803C>G p.P268R EX7 3.1% missensePE620 RELN c.1047G>T p.L349F EX10 10.8% missensePE881 CIC c.4486C>T p.R1496C EX20E 1.3% missensePE875 ALK c.1528C>T p.R510W EX7 9.0% missensePE796 EGFR c.2573T>G p.L858R EX21 58.4% missensePE887 ATM c.8149A>G p.K2717E EX55 1.0% missensePE923 MLL3 c.3627A>G p.I1209M EX23 1.5% missensePE655 AR c.2169G>C p.L723F EX4 21.9% missensePE269 KDM5C c.704A>G p.K235R EX6 1.1% missensePE274 EGFR c.2369C>T p.T790M EX20 6.9% missensePE252 BCOR c.4376A>G p.N1459S EX10 38.8% missensePE349 MS4A1 c.491T>A p.I164K EX6 8.6% missensePE287 EPHA5 c.1933C>A p.P645T EX11 3.4% missensePE331 AFF3 c.1856_1862delCCTCAGCinsGp.T619_A621delinsSEX14 1.4% cds-indelPE395 KRAS c.35G>C p.G12A EX2 38.4% missensePE424 FANCA c.1498C>T p.P500S EX16 1.1% missensePE368 HSP90AA1 c.985G>T p.V329L EX5 1.4% missense

PE457 TP53 c.488A>G p.Y163C EX5 1.3% missensePE550 ZFP36L1 c.713A>G p.Y238C EX3E 2.6% missensePE442 NOTCH2 c.454C>A p.P152T EX4 5.7% missensePE575 PDGFRB c.1811G>A p.R604H EX13 0.6% missensePE528 TP53 c.451C>G p.P151A EX5 34.5% missensePE578 TP53 c.919+1G>T . IVS8 54.3% splice-5PE604 EGFR c.2573T>G p.L858R EX21 0.7% missensePE510 NFE2L2 c.89T>G p.L30R EX2 37.7% missensePE551 SMARCA4 c.3702C>A p.F1234L EX26 36.0% missensePE672 PIK3C2G c.4180G>A p.E1394K EX31 1.8% missensePE496 EGFR c.2235_2249delGGAATTAAGAGAAGCp.E746_A750del EX19 17.4% cds-delPE701 STAT3 c.1346G>T p.G449V EX15 2.6% missensePE669 RET c.2183A>T p.K728I EX12 1.0% missensePE689 PTK2 c.3188G>C p.R1063T EX33E 1.9% missensePE793 ZFHX3 c.5861T>G p.F1954C EX9 8.9% missensePE883 PTPRD c.2021T>C p.L674S EX24 1.3% missensePE910 NBN c.1528G>A p.E510K EX11 3.5% missensePE893 ABL2 c.337G>A p.V113I EX3 16.3% missensePE870 PALB2 c.3355C>G p.L1119V EX13E 6.0% missensePE919 NTRK1 c.286C>A p.L96I EX2 1.2% missensePE293 TP53 c.946C[4>3] p.Q317Sfs*28 EX9 88.1% frameshiftPE356 FASLG c.266T>C p.M89T EX1 12.3% missensePE346 KRAS c.35G>T p.G12V EX2 5.5% missensePE455 PIK3C2G c.4180G>A p.E1394K EX31 1.0% missensePE406 TP53 c.63C[2>1] p.L22Yfs*22 EX2 2.7% frameshiftPE540 TBX3 c.1700C>T p.A567V EX7 17.7% missensePE552 TP53 c.742C>T p.R248W EX7 88.5% missensePE701 EGFR c.2239_2248delinsCp.L747_A750delinsPEX19 41.2% cds-indelPE719 LRP1B c.3697C>T p.H1233Y EX23 7.6% missensePE689 BCOR c.4672T[2>3] p.L1558Ffs*7 EX12 2.6% frameshiftPE765 SMAD4 c.1157G>A p.G386D EX10 0.6% missensePE657 MLL3 c.5633C>G p.S1878* EX36 1.6% nonsensePE735 NF2 c.1021C>T p.R341* EX11 56.8% nonsensePE895 SF3B1 c.2221A>C p.K741Q EX15 45.0% missensePE592 MIB1 c.817G>C p.D273H EX6 5.1% missensePE918 TP53 c.653T>G p.V218G EX6 23.0% missensePE210 HDAC4 c.2315G>A p.R772H EX18 5.6% missensePE223 SNTG1 c.609G>T p.W203C EX11 31.3% missensePE263 ERBB3 c.2011A>T p.I671F EX17 1.6% missensePE345 EGFR c.2239_2260delinsGCAACATCTCCGGp.L747_K754delinsATSPEEX19 41.6% cds-indelPE207 ERRFI1 c.157G>A p.A53T EX3 5.0% missensePE279 DNMT3A c.2408+2T>C . IVS20 0.9% splice-5PE313 RNF43 c.367G>C p.A123P EX3 8.9% missensePE339 RB1 c.718A>T p.K240* EX7 43.7% nonsensePE379 IGF1R c.3043G>C p.E1015Q EX16 3.1% missensePE429 RAF1 c.403C>G p.P135A EX4 33.2% missensePE525 ATR c.5102C>G p.S1701C EX29 5.4% missensePE584 MAP2K4 c.404C>G p.S135* EX4 3.4% nonsensePE594 CDH23 c.9859G>A p.G3287S EX70E 14.2% missensePE639 EPHA5 c.1420A>G p.N474D EX6 3.0% missensePE509 ARID1A c.2248C>T p.R750* EX6 38.2% nonsensePE629 PTEN c.379G>A p.G127R EX5 78.0% missensePE437 FLT1 c.1194G>C p.E398D EX9 30.0% missensePE771 EIF1AX c.406G>A p.D136N EX6 10.0% missense

PE840 NOTCH2 c.2602C>T p.Q868* EX17 1.9% nonsensePE875 MLL2 c.2742_2743delGCinsTTp.E914D EX10 0.8% missensePE360 STAG2 c.2501T>C p.F834S EX25 1.0% missensePE631 ERCC3 c.891C>A p.F297L EX7 4.5% missensePE828 FGF10 c.402G>T p.M134I EX2 32.8% missensePE422 FAT1 c.4535G>A p.G1512D EX8 0.4% missensePE218 TP53 c.394A>C p.K132Q EX5 0.7% missensePE319 EZH2 c.2111T>A p.V704D EX19 1.2% missensePE322 EP300 c.4195G>T p.D1399Y EX26 2.8% missensePE400 LRP1B c.4973G>T p.W1658L EX30 6.3% missensePE391 KDM5C c.802C[6>7] p.T270Hfs*20 EX7 12.6% frameshiftPE364 JAK1 c.1957C>G p.L653V EX14 1.0% missensePE457 CDC73 c.1451G>T p.R484L EX16 4.0% missensePE523 DICER1 c.5372G>C p.R1791T EX26 16.0% missensePE588 EGFR c.2235_2249delGGAATTAAGAGAAGCp.E746_A750del EX19 55.0% cds-delPE672 SUFU c.686C>T p.A229V EX6 2.1% missensePE737 BCOR c.4621G>T p.D1541Y EX12 94.4% missensePE671 TP53 c.469G>T p.V157F EX5 50.6% missensePE698 MST1R c.2050C>T p.P684S EX7 28.2% missensePE650 RBM10 c.2119A>T p.K707* EX17 74.1% nonsensePE678 HGF c.1024G>A p.E342K EX8 3.8% missensePE812 MAP2K4 c.376C>G p.Q126E EX3 6.1% missensePE748 TBX3 c.1398G>T p.K466N EX7 8.3% missensePE877 INPP4B c.1932G>C p.Q644H EX20 3.3% missensePE826 EGFR c.2369C>T p.T790M EX20 0.1% missensePE591 U2AF1 c.101C>T p.S34F EX2 28.8% missensePE821 CUL3 c.227G>T p.G76V EX2 7.6% missensePE632 WHSC1 c.877T>G p.L293V EX4 1.9% missensePE931 EPAS1 c.2566C>T p.L856F EX16E 1.3% missensePE222 RICTOR c.2053C>G p.L685V EX22 20.4% missensePE211 EGFR c.2235_2249delGGAATTAAGAGAAGCp.E746_A750del EX19 62.1% cds-delPE260 CTCF c.121C>G p.P41A EX3 2.9% missensePE213 EGFR c.2582T>G p.L861R EX21 65.2% missensePE207 EPHB4 c.2534G>A p.C845Y EX15 9.4% missensePE339 SPSB4 c.553G>A p.E185K EX2 6.2% missensePE320 MUTYH c.1118C>T p.A373V EX12 1.4% missensePE370 FBXW7 c.1544T>C p.V515A EX10 54.9% missensePE543 NAV3 c.3043A>G p.K1015E EX15 7.0% missensePE518 TP53 c.1024C>T p.R342* EX10 5.3% nonsensePE594 NOTCH4 c.813_815delAGAinsGGGp.D272G EX5 9.6% missensePE581 EPHA5 c.106C>T p.R36* EX1 35.0% nonsensePE623 ERBB2 c.2332_2340dupGGCTCCCCAp.G778_P780dup EX20 0.3% cds-insPE634 EGFR c.2240_2257delTAAGAGAAGCAACATCTCp.L747_P753delinsSEX19 12.3% cds-indelPE679 RECQL4 c.907G>A p.V303I EX5 5.0% missensePE832 SOX2 c.590T>G p.M197R EX1E 2.9% missensePE715 SLC34A2 c.413C>A p.S138Y EX5 1.9% missensePE806 KRAS c.34G>T p.G12C EX2 91.6% missensePE620 CTNNB1 c.110C>G p.S37C EX3 10.1% missensePE790 NF2 c.1271G>A p.R424H EX12 0.9% missensePE900 ERG c.242C>T p.S81F EX3 1.1% missensePE928 NFKBIA c.394G>A p.G132R EX3 5.2% missensePE923 RAD54L c.2063G>A p.R688H EX19E 2.0% missensePE861 TP53 c.560-2A>T . IVS5 0.5% splice-3PE655 KRAS c.35G>A p.G12D EX2 29.7% missense

PE250 STAT3 c.797+1G>T . IVS8 6.6% splice-5PE254 CTNNB1 c.94G>A p.D32N EX3 0.8% missensePE276 FAT2 c.235G>A p.A79T EX1 17.0% missensePE243 EGFR c.2235_2249delGGAATTAAGAGAAGCp.E746_A750del EX19 37.4% cds-delPE207 FAT1 c.8319G>A p.W2773* EX10 24.5% nonsensePE431 EGFR c.2369C>T p.T790M EX20 0.7% missensePE394 POLE c.1020+1G>T . IVS10 20.8% splice-5PE498 FAT2 c.8080C>T p.P2694S EX9 0.8% missensePE500 SMAD4 c.1309-1G>T . IVS10 17.3% splice-3PE507 EGFR c.2573T>G p.L858R EX21 28.8% missensePE654 PAX5 c.113G>A p.R38H EX2 71.5% missensePE728 MLL3 c.604_605delCAinsTp.Q202Cfs*4 EX5 2.8% frameshiftPE767 BRCA1 c.4800G>C p.L1600F EX15 1.0% missensePE791 CYBA c.223G>A p.A75T EX4 0.6% missensePE810 EGFR c.2235_2249delGGAATTAAGAGAAGCp.E746_A750del EX19 6.7% cds-delPE891 BBC3 c.667C>T p.R223* EX4E 5.1% nonsensePE222 PTEN c.963A[6>7] p.N323Kfs*2 EX8 7.5% frameshiftPE211 TP53 c.523C>G p.R175G EX5 55.7% missensePE222 EGFR c.2573T>G p.L858R EX21 1.6% missensePE260 CASP8 c.1570C>A p.Q524K EX9 2.5% missensePE213 ACVR1B c.1580C>T p.T527M EX10 17.9% missensePE207 FN1 c.2081G>A p.R694H EX14 9.4% missensePE339 ARAP3 c.1537G>A p.D513N EX10 1.8% missensePE415 JAK2 c.1849G>T p.V617F EX14 2.0% missensePE320 AFF3 c.1982G>A p.S661N EX14 1.3% missensePE500 MET c.3028+2T>A . IVS14 10.0% splice-5PE543 TP53 c.673-2A>C . IVS6 11.0% splice-3PE518 RBM10 c.1813C>T p.Q605* EX15 5.1% nonsensePE594 PRDM1 c.1633G>T p.E545* EX5 27.1% nonsensePE581 MAP3K1 c.455A>T p.E152V EX1 90.2% missensePE634 ABCB1 c.1116G>T p.K372N EX12 3.6% missensePE695 BRAF c.1799T>A p.V600E EX15 3.0% missensePE832 EGFR c.2573T>G p.L858R EX21 40.0% missensePE710 CUL3 c.265-2A>G . IVS2 16.4% splice-3PE715 RSPO2 c.428-2A>T . IVS4 1.5% splice-3PE806 FANCA c.1226-1G>A . IVS13 38.7% splice-3PE889 AXL c.1472G>C p.R491T EX12 13.4% missensePE881 EGFR c.2573T>G p.L858R EX21 4.5% missensePE360 CDKN2A c.220G>A p.D74N EX2 4.2% missensePE928 ERCC4 c.1198G>T p.A400S EX7 1.5% missensePE655 PALB2 c.3367G>A p.V1123M EX13E 15.1% missensePE218 MLL2 c.11582A>T p.Q3861L EX39 0.6% missensePE304 DNMT3A c.890G>A p.W297* EX8 1.0% nonsensePE322 CYP2D6 c.568A>T p.T190S EX4 2.2% missensePE391 MAP3K1 c.2746T>G p.C916G EX14 5.1% missensePE424 NOTCH1 c.2972C>T p.S991F EX19 1.3% missensePE457 LRP1B c.9731C>T p.S3244L EX61 0.5% missensePE523 FAT1 c.6424G>C p.E2142Q EX10 11.9% missensePE576 TP53 c.742C>T p.R248W EX7 89.6% missensePE421 TP53 c.851_855delCAGAGinsAp.T284Kfs*60 EX8 61.0% frameshiftPE474 MLL3 c.13166C[3>2] p.K4391Nfs*22 EX52 0.6% frameshiftPE588 PIK3CG c.374G>C p.W125S EX2 2.3% missensePE440 PTEN c.951ACTT[2>1] p.T319*fs*1 EX8 42.5% frameshiftPE689 PIK3CB c.1438C>T p.Q480* EX9 1.2% nonsense

PE799 EGFR c.2240_2254delTAAGAGAAGCAACATp.L747_T751del EX19 36.4% cds-delPE667 EGFR c.2369C>T p.T790M EX20 1.6% missensePE812 CIC c.1135-1G>T . IVS7 5.8% splice-3PE748 ABCC11 c.3712C>G p.P1238A EX27 8.9% missensePE821 TGFBR2 c.1212C>A p.D404E EX5 1.0% missensePE632 FAT1 c.2974G>A p.E992K EX2 1.2% missensePE931 CTNNB1 c.98C>G p.S33C EX3 5.0% missensePE223 TP53 c.536A>G p.H179R EX5 2.7% missensePE231 EGFR c.2235_2249delGGAATTAAGAGAAGCp.E746_A750del EX19 15.6% cds-delPE274 CDK13 c.4123C>T p.Q1375* EX14 8.5% nonsensePE252 CHD4 c.2374C>T p.R792W EX16 41.0% missensePE349 EGFR c.2573T>G p.L858R EX21 4.5% missensePE341 ERBB2 c.2313_2324dupATACGTGATGGCp.Y772_A775dup EX20 15.7% cds-insPE402 TP53 c.659A>G p.Y220C EX6 86.1% missensePE400 KDM5C c.3285C>G p.C1095W EX21 7.4% missensePE410 SETBP1 c.4315C>A p.L1439M EX6E 33.5% missensePE395 PIK3CA c.3140A>T p.H1047L EX21E 19.2% missensePE368 EGFR c.2239_2240delTTinsCCp.L747P EX19 12.6% missensePE364 MET c.608C>T p.S203F EX2 8.3% missensePE340 HCN1 c.2608G>A p.E870K EX8E 1.0% missensePE457 FANCM c.1679G>C p.C560S EX10 1.3% missensePE396 TP53 c.380C>T p.S127F EX5 3.5% missensePE550 EGFR c.2235_2249delGGAATTAAGAGAAGCp.E746_A750del EX19 4.2% cds-delPE581 CCNE1 c.994G>C p.V332L EX11 62.9% missensePE551 RAF1 c.349G>A p.D117N EX4 28.7% missensePE553 AR c.1759G>T p.A587S EX2 37.1% missensePE669 EGFR c.2573T>G p.L858R EX21 15.3% missensePE689 EPHB6 c.101-1G>C . IVS5 1.0% splice-3PE753 RAC1 c.19G>A p.V7M EX1 26.7% missensePE911 NF2 c.1564G>T p.E522* EX14 3.1% nonsensePE885 TP53 c.578A>T p.H193L EX6 21.4% missensePE822 U2AF1 c.101C>T p.S34F EX2 31.0% missensePE870 EGFR c.2369C>T p.T790M EX20 25.7% missensePE816 FANCE c.1593G>C p.L531F EX10E 1.1% missensePE284 TP53 c.843C>A p.D281E EX8 1.5% missensePE305 PCDH18 c.2729G>A p.R910K EX3 1.0% missensePE424 MLL3 c.11590C>T p.R3864C EX44 1.1% missensePE534 SETD2 c.4616G>A p.C1539Y EX5 3.5% missensePE523 EPHA3 c.2944C>T p.P982S EX17E 27.4% missensePE638 ATRX c.2156C>G p.S719C EX9 1.8% missensePE587 MED12 c.3128T>G p.L1043R EX22 24.6% missensePE440 HCLS1 c.542C>T p.T181M EX7 15.6% missensePE514 MLL c.230G>A p.G77E EX1 7.2% missensePE625 DUSP22 c.282G>C p.R94S EX6 2.1% missensePE737 GRIN2A c.998C>A p.T333N EX3 18.8% missensePE690 WDR90 c.4379G>A p.R1460Q EX35 8.2% missensePE761 PDGFRA c.3118C>G p.H1040D EX22 11.4% missensePE698 ARID1A c.2141TC[2>1] p.S715Afs*101 EX5 3.4% frameshiftPE687 SMARCA4 c.2164C>T p.Q722* EX15 3.9% nonsensePE650 EGFR c.2389T>A p.C797S EX20 2.3% missensePE667 CTNNB1 c.274C>G p.Q92E EX4 2.4% missensePE812 NOTCH2 c.4573G>A p.E1525K EX26 7.3% missensePE676 ACIN1 c.1940_1941insTCGTACp.S647_A648insRTEX6 0.7% cds-insPE877 EPHB2 c.1785C>G p.I595M EX10 3.7% missense

PE872 AXL c.854C>T p.S285L EX7 68.6% missensePE833 EGFR c.2235_2249delGGAATTAAGAGAAGCp.E746_A750del EX19 19.0% cds-delPE930 EGFR c.2239_2248delinsCp.L747_A750delinsPEX19 2.8% cds-indelPE206 RNASEL c.131_138delTGCTGGAAinsGp.L44Wfs*24 EX2 1.2% frameshiftPE247 EGFR c.2573T>G p.L858R EX21 0.6% missensePE464 RHOA c.118G>C p.E40Q EX2 4.5% missensePES121 TP53 c.517G>T p.V173L EX5 3.2% missensePES121 TP53 c.517G>T p.V173L EX5 4.4% missensePE638 ROBO3 c.1390C>G p.L464V EX9 2.4% missensePE587 TP53 c.417G>T p.K139N EX5 0.5% missensePE690 ZNF384 c.701C>T p.S234L EX7 1.0% missensePE471 JAK3 c.2047+1G>T . IVS15 0.5% splice-5PE834 CHFR c.856G>A p.E286K EX8 0.7% missensePE840 TOP2A c.268+1delG . IVS3 1.7% splice-5PE848 TOP1 c.578A>G p.K193R EX8 0.5% missensePE734 BCOR c.2567dupG p.E857Rfs*8 EX4 1.1% frameshiftPE839 ZFHX3 c.5023C>G p.P1675A EX9 15.8% missensePE902 LRRC7 c.2261G>A p.R754H EX19 1.7% missensePE872 EGFR c.2573T>G p.L858R EX21 32.4% missensePE937 FOXP1 c.517C>T p.Q173* EX6 0.5% nonsensePE930 RAD51C c.193A>G p.R65G EX2 3.8% missensePE296 GATA3 c.620C[3>2] p.L208Wfs*57 EX3 1.3% frameshiftPE376 BRAF c.1397G>T p.G466V EX11 0.8% missensePE424 ROBO3 c.412G>A p.E138K EX2 1.0% missensePE364 NFE2L2 c.72G>C p.W24C EX2 2.0% missensePE457 FLT4 c.1355T>A p.L452Q EX10 1.1% missensePE523 TBX3 c.1355A>G p.E452G EX7 1.4% missensePE547 BRCA2 c.441A>T p.Q147H EX5 1.3% missensePE421 MSN c.1624G>A p.D542N EX13E 1.1% missensePE603 KRAS c.37G>T p.G13C EX2 0.8% missensePE588 FANCM c.5230G>A p.E1744K EX20 17.2% missensePE440 IRS2 c.3736G>A p.G1246S EX1 7.4% missensePE690 SOX9 c.1073C>G p.A358G EX3E 2.6% missensePE689 FBXW7 c.1465G>C p.E489Q EX10 1.1% missensePE740 MSH2 c.373C>T p.P125S EX3 2.3% missensePE698 EGFR c.2240_2254delTAAGAGAAGCAACATp.L747_T751del EX19 13.9% cds-delPE678 TP53 c.490A[2>3] p.Q165Afs*16 EX5 3.0% frameshiftPE748 SMARCB1 c.470G>T p.G157V EX4 12.2% missensePE877 EGFR c.2573T>G p.L858R EX21 20.1% missensePE821 TP53 c.148dupA p.I50Nfs*2 EX4 4.2% frameshiftPE632 KRAS c.34G>T p.G12C EX2 4.4% missensePE903 RET c.2302G>A p.E768K EX13 2.3% missensePE255 DNMT3A c.1940T>C p.L647P EX17 0.5% missensePE252 TGFBR2 c.1634G>T p.C545F EX8 44.5% missensePE327 DNMT3A c.2353G>A p.V785M EX20 1.1% missensePE346 TP53 c.699_701delCTAinsGTGp.H233_Y234delinsQCEX7 32.8% missensePE402 KRAS c.523G>A p.E175K EX5 40.3% missensePE400 NOTCH3 c.5948G>A p.G1983D EX33 14.7% missensePE410 KALRN c.2466C>A p.N822K EX14 21.5% missensePE323 EPHB6 c.2131A>T p.S711C EX15 2.8% missensePE489 RYR2 c.6861C>A p.D2287E EX45 2.7% missensePE457 KRAS c.181C>G p.Q61E EX3 3.0% missensePE396 APC c.5027G>C p.R1676T EX16E 2.4% missensePE588 MED12 c.3128T>G p.L1043R EX22 79.7% missense

PE599 ALK c.3806G>C p.G1269A EX25 3.4% missensePE712 TP53 c.811G>T p.E271* EX8 12.9% nonsensePE630 EGFR c.2154_2155delGGinsTTp.G719C EX18 82.8% missensePE711 ERBB4 c.3589A>C p.K1197Q EX28E 16.9% missensePE763 AURKB c.342G>T p.E114D EX5 10.6% missensePE740 TP53 c.919+1delG . IVS8 7.4% splice-5PE698 NTRK3 c.338C>T p.S113L EX5 3.6% missensePE793 EPHA5 c.878C>A p.A293E EX3 19.6% missensePE885 RECQL c.220C>G p.P74A EX4 13.2% missensePE735 CARD11 c.1323C>G p.D441E EX9 10.9% missensePE935 TP53 c.614A>G p.Y205C EX6 32.8% missensePES130 PDGFRB c.2614C>G p.P872A EX19 6.0% missensePE212 LRP1B c.6491C>G p.S2164C EX41 2.2% missensePE260 ERBB2 c.2313_2324dupATACGTGATGGCp.Y772_A775dup EX20 1.5% cds-insPE207 IGF1R c.2827G>A p.A943T EX14 8.2% missensePE357 TP53 c.991C>T p.Q331* EX9 0.6% nonsensePE606 TP53 c.818G>T p.R273L EX8 47.0% missensePE339 EGFR c.2369C>T p.T790M EX20 0.6% missensePE320 DDR1 c.2442G>T p.E814D EX18 20.6% missensePE434 MET c.3209T>C p.V1070A EX15 6.9% missensePE359 SF3B1 c.1996A>G p.K666E EX14 16.9% missensePE764 CDK13 c.872C>T p.S291L EX1 10.2% missensePE718 MET c.3028+1G>T . IVS14 4.2% splice-5PE832 TP53 c.880G>T p.E294* EX8 18.4% nonsensePE849 AFF3 c.1907C>T p.A636V EX14 1.3% missensePE710 MET c.3689A>C p.Y1230S EX19 22.3% missensePE620 NF1 c.3095G>T p.C1032F EX23 8.6% missensePE875 FAT1 c.3864_3873delCTACAGCATCp.Y1289Kfs*76 EX5 3.3% frameshiftPE360 TP53 c.517G>T p.V173L EX5 9.1% missensePE912 EGFR c.2235_2249delGGAATTAAGAGAAGCp.E746_A750del EX19 52.8% cds-delPE223 KDM6A c.3637C>T p.R1213* EX25 13.5% nonsensePE207 FGFR4 c.1413G>T p.K471N EX11 2.4% missensePE280 KRAS c.351A>T p.K117N EX4 0.8% missensePE313 ALK c.2816G>A p.G939D EX17 2.2% missensePE339 NF1 c.1831C>T p.L611F EX16 0.6% missensePE412 ABCA13 c.13764G>A p.M4588I EX52 4.5% missensePE548 EGFR c.2236_2250delGAATTAAGAGAAGCAp.E746_A750del EX19 29.3% cds-delPE555 FCGR2A c.94G>A p.D32N EX2 3.3% missensePE525 LRP4 c.1933G>A p.A645T EX15 6.6% missensePE520 EGFR c.2236_2250delGAATTAAGAGAAGCAp.E746_A750del EX19 46.3% cds-delPE594 POLE c.3614C>T p.P1205L EX30 27.7% missensePE516 EGFR c.2573T>G p.L858R EX21 35.3% missensePE509 FAT2 c.8990T>C p.V2997A EX10 8.4% missensePE572 TP73 c.350C>T p.S117F EX4 66.8% missensePE437 TP53 c.742C>T p.R248W EX7 48.0% missensePE755 BRCA2 c.2476G>C p.E826Q EX11 8.2% missensePE849 ZFHX3 c.10005G>A p.M3335I EX10E 1.0% missensePE491 NFE2L2 c.85G>T p.D29Y EX2 45.3% missensePE631 RB1 c.91G>T p.E31* EX1 66.4% nonsensePE693 ERBB3 c.3010G>C p.E1004Q EX25 1.4% missensePE220 MLL2 c.10328G>T p.S3443I EX35 8.4% missensePE222 EGFR c.2235_2249delGGAATTAAGAGAAGCp.E746_A750del EX19 26.0% cds-delPE336 TP53 c.672+1G>T . IVS6 44.1% splice-5PE324 TP53 c.749C>T p.P250L EX7 45.3% missense

PE207 ELMO1 c.1558T>C p.S520P EX17 1.9% missensePE338 EGFR c.2236_2250delGAATTAAGAGAAGCAp.E746_A750del EX19 11.0% cds-delPE363 PDGFRB c.2960G>A p.R987Q EX22 3.4% missensePE370 DNMT3A c.2408+1G>A . IVS20 1.5% splice-5PE465 TP53 c.530C>A p.P177H EX5 80.5% missensePE417 DNMT3A c.1455G>C p.Q485H EX12 27.2% missensePE555 SETD2 c.1102C>T p.R368* EX3 2.7% nonsensePE520 ACIN1 c.3360delAinsCCCCCCCp.P1119[6>8] EX17 37.1% cds-insPE531 PTGS2 c.1066_1067insTGGGTp.Q356Lfs*91 EX8 2.1% frameshiftPE594 FANCA c.1607C>T p.S536L EX17 20.2% missensePE509 MLL3 c.7801G>C p.G2601R EX38 41.5% missensePE613 TET2 c.2021delA p.Q674Rfs*26 EX3 4.6% frameshiftPE690 ARID1A c.3276G>C p.L1092F EX12 1.0% missensePE783 WWP1 c.686C>T p.A229V EX8 36.1% missensePE679 CREBBP c.3227C>T p.S1076F EX16 11.4% missensePE662 CUL3 c.772G>T p.E258* EX6 1.8% nonsensePE749 FAT1 c.4184-1G>T . IVS6 9.7% splice-3PE857 AKT1 c.1221C>G p.F407L EX12 16.0% missensePE491 PIK3CA c.1633G>A p.E545K EX10 40.6% missensePE875 POLD1 c.395A>C p.D132A EX4 1.1% missensePE828 FAT3 c.12773G>T p.G4258V EX23 36.0% missensePE693 CDH1 c.136C>G p.L46V EX2 12.9% missensePE219 EGFR c.2369C>T p.T790M EX20 1.4% missensePE325 TPR c.5098A>G p.M1700V EX36 10.2% missensePE336 PIK3CA c.1564G>A p.E522K EX10 20.0% missensePE215 MNDA c.703A>G p.M235V EX5 22.7% missensePE403 FANCD2 c.670C>G p.H224D EX9 4.9% missensePE436 EPHA3 c.2326G>T p.E776* EX13 4.0% nonsensePE388 ARAP3 c.1537G>A p.D513N EX10 1.1% missensePE428 EP300 c.2276C>T p.S759F EX13 4.7% missensePE417 RAD50 c.473A>T p.H158L EX4 30.4% missensePE473 EGFR c.2236_2250delGAATTAAGAGAAGCAp.E746_A750del EX19 26.6% cds-delPE524 RBM10 c.2572C>T p.R858* EX21 47.3% nonsensePE601 PIK3C2G c.4180G>A p.E1394K EX31 1.0% missensePE505 MLL2 c.8485A>T p.M2829L EX34 7.6% missensePE586 TP53 c.380C>T p.S127F EX5 66.2% missensePE572 HGF c.647G>T p.G216V EX6 21.6% missensePE702 SLC34A2 c.2049A[3>4] p.T685Dfs*21 EX13E 14.8% frameshiftPE599 ARID1A c.3635_3638delAGCCp.Q1212Pfs*3 EX14 66.3% frameshiftPE480 TP53 c.659A>G p.Y220C EX6 16.0% missensePE622 SETD2 c.5445G>T p.L1815F EX12 1.0% missensePE697 PBRM1 c.3800+1G>T . IVS24 21.1% splice-5PE749 IGF1R c.346G>A p.A116T EX2 2.0% missensePE840 NSD1 c.7861G>C p.E2621Q EX23E 5.2% missensePE774 TP53 c.920-2A>G . IVS8 8.2% splice-3PE858 NTRK1 c.1315C>A p.L439I EX11 23.3% missensePE900 TAF1 c.3805G>A p.E1269K EX24 1.1% missensePE303 EP300 c.5261C>G p.S1754* EX31 5.2% nonsensePE301 TERT c.-58-u66C>T . . 13.9% promoterPE207 TCF7L2 c.1395A[9>8] p.K468Sfs*23 EX14 17.4% frameshiftPE416 MLL c.9591_9602delCCAACTTTTGGTp.Q3198_V3201delEX27 2.2% cds-delPE388 KEAP1 c.1238G>T p.R413L EX3 6.1% missensePES121 NF1 c.2431A>T p.T811S EX21 0.5% missensePE493 TP53 c.305C[2>1] p.Y103Tfs*20 EX4 4.8% frameshift

PE586 KDM5A c.5025C>G p.F1675L EX28E 34.7% missensePE672 ZFHX3 c.10389C>G p.Y3463* EX10E 6.4% nonsensePE354 TP53 c.637C>T p.R213* EX6 20.8% nonsensePE732 EGFR c.2303G>T p.S768I EX20 13.5% missensePE679 ERBB4 c.2230G>C p.V744L EX19 10.6% missensePE857 CLDN18 c.168G>C p.Q56H EX1 23.6% missensePE807 ALK c.3806G>C p.G1269A EX25 2.0% missensePE856 EGFR c.2235_2249delGGAATTAAGAGAAGCp.E746_A750del EX19 38.9% cds-delPE922 TGFBR2 c.1283G>A p.R428H EX5 6.5% missensePE222 CDKN2A c.133G[2>1] p.G45Vfs*8 EX1 21.1% frameshiftPE211 SMARCA4 c.3563A>T p.D1188V EX26 57.8% missensePE260 FAT1 c.6917G>C p.R2306T EX10 2.6% missensePE213 TP53 c.578A>G p.H193R EX6 43.7% missensePE207 ATR c.3396T[7>8] p.N1135*fs*1 EX17 9.4% frameshiftPE339 TET2 c.847G>C p.E283Q EX3 3.0% missensePE415 EGFR c.2573T>G p.L858R EX21 0.7% missensePE320 ALK c.357G>T p.E119D EX1 2.2% missensePE483 SF3B1 c.2219G>A p.G740E EX15 17.9% missensePE489 SMAD4 c.1569C>G p.C523W EX12E 2.8% missensePE500 MET c.3007_3028+1delTACCGAGCTACTTTTCCAGAAGG. EX14-IVS142.6% spanPE543 PTPN11 c.1105A>G p.K369E EX10 6.5% missensePE594 CCND3 c.826C>T p.Q276* EX5E 19.2% nonsensePE581 FAT1 c.8998G>A p.D3000N EX11 72.1% missensePE634 EGFR c.2497T>G p.L833V EX21 14.6% missensePE731 TP53 c.376-1G>A . IVS4 39.4% splice-3PE718 SLIT2 c.3183-2A>G . IVS30 1.9% splice-3PE696 MET c.3028+352G>A . IVS14 4.3% intronPE730 ACVR1B c.205T>G p.C69G EX2 4.2% missensePE755 SETD2 c.2893G>A p.E965K EX3 13.4% missensePE832 PMS2 c.1687C>T p.R563* EX11 21.2% nonsensePE715 FLT4 c.2128G>A p.V710M EX14 4.6% missensePE806 GRIN2A c.1983A>T p.Q661H EX9 4.7% missensePE889 RNF43 c.1820C>T p.S607L EX9 2.1% missensePE881 EGFR c.2369C>T p.T790M EX20 1.0% missensePE790 STAG2 c.1264C>T p.R422W EX14 0.8% missensePE360 EGFR c.2573T>G p.L858R EX21 6.1% missensePE928 MAP2K1 c.538C>G p.L180V EX5 5.4% missensePE861 SMAD4 c.1333C>T p.R445* EX11 0.5% nonsensePE914 BTK c.1735G>C p.D579H EX17 2.4% missensePE223 DNMT3A c.1228G>A p.A410T EX10 1.6% missensePE222 ABCC11 c.3868A>T p.R1290W EX28 3.1% missensePE251 CYLD c.2581G>T p.V861F EX17 0.8% missensePE352 TP53 c.402T>G p.F134L EX5 78.8% missensePE362 FGFR1 c.566G>A p.R189H EX5 1.6% missensePE460 EGFR c.2573T>G p.L858R EX21 39.1% missensePE394 ASH1L c.407_420+15delGTCCTTCAAAACGAGTAAGTAGAATTCCT. EX2-IVS2 5.8% spanPE448 SMARCE1 c.461A>G p.E154G EX7 1.4% missensePE500 MSH2 c.1183C>G p.Q395E EX7 27.0% missensePE539 RAF1 c.779C>A p.T260K EX7 51.4% missensePE594 TP53 c.481G>A p.A161T EX5 4.0% missensePE593 BARD1 c.1518_1519delTGinsCAp.V507M EX6 33.5% missensePE643 PIK3R2 c.2017A>G p.T673A EX16E 7.1% missensePE728 IDH1 c.262T>G p.L88V EX4 0.8% missensePE731 NOTCH2 c.7093G>A p.V2365I EX34E 5.0% missense

PE610 GAB2 c.386G>C p.R129T EX3 2.1% missensePE680 MED12 c.4363C>A p.Q1455K EX31 15.3% missensePE819 EGFR c.2573T>G p.L858R EX21 1.5% missensePE495 RECQL4 c.1276G>C p.D426H EX7 1.4% missensePE742 KDR c.355C>T p.Q119* EX3 26.1% nonsensePE915 ERCC5 c.2965A>T p.T989S EX15E 19.6% missensePE910 ERBB2 c.2313_2324dupATACGTGATGGCp.Y772_A775dup EX20 7.9% cds-insPE891 WRN c.4111A>C p.K1371Q EX34 2.3% missensePE935 PBRM1 c.3724C>T p.Q1242* EX24 27.1% nonsensePE424 FAT2 c.9893C>T p.S3298F EX14 1.4% missensePES121 PMS1 c.1948G>T p.G650C EX10 0.5% missensePE520 EGFR c.2369C>T p.T790M EX20 5.6% missensePE726 EGFR c.2369C>T p.T790M EX20 14.2% missensePE687 DAXX c.953G>T p.R318L EX3 3.0% missensePE880 TP53 c.830G>T p.C277F EX8 45.4% missensePE798 COPS2 c.683C>T p.S228L EX7 1.1% missensePE491 TP53 c.470T>C p.V157A EX5 60.1% missensePE909 RBM10 c.1576A>T p.K526* EX13 30.8% nonsensePE913 TP53 c.701A>G p.Y234C EX7 31.6% missensePE252 CTNNB1 c.110C>T p.S37F EX3 4.2% missensePE270 EPAS1 c.622C>T p.H208Y EX6 15.7% missensePE334 TP53 c.482C>A p.A161D EX5 48.1% missensePE264 TBX22 c.77A>C p.D26A EX2 39.2% missensePE378 MTRR c.38G>T p.G13V EX2 4.7% missensePE464 GRIN2A c.631G>C p.E211Q EX3 4.4% missensePE468 TP53 c.826_827delGCinsTTp.A276F EX8 93.3% missensePE477 EPHA5 c.2819G>T p.S940I EX16 4.6% missensePE506 EP300 c.4780-1G>A . IVS29 44.2% splice-3PE442 CTNNB1 c.1004A>T p.K335I EX7 3.4% missensePE517 RBM10 c.1813C>T p.Q605* EX15 1.9% nonsensePE636 EGFR c.2235_2249delGGAATTAAGAGAAGCp.E746_A750del EX19 19.8% cds-delPE654 CASP8 c.20C>T p.A7V EX1 15.6% missensePE613 ERBB2 c.2326_2327insTCGTGATGGCTGp.A775_G776insVVMAEX20 49.4% cds-insPE628 OR4A15 c.545T>G p.L182R EX1E 7.8% missensePE660 BCOR c.444A>G p.I148M EX4 5.5% missensePE689 RB1 c.341C>T p.S114L EX3 2.7% missensePE384 ERG c.242C>T p.S81F EX3 0.5% missensePE757 ATRX c.995A>G p.D332G EX9 77.3% missensePE787 EGFR c.2390G>C p.C797S EX20 0.6% missensePE840 MLL3 c.6007A>G p.T2003A EX36 0.8% missensePE759 CARD11 c.8G>T p.G3V EX3 1.5% missensePE888 BCL9 c.3128_3131delGTTCp.R1043Lfs*7 EX9 7.7% frameshiftPE921 EGFR c.2573T>G p.L858R EX21 2.7% missensePE308 AR c.2108C>T p.S703F EX4 1.8% missensePE216 PTEN c.496_497delGTinsCAp.V166Q EX6 2.8% missensePE303 FANCM c.2483C>T p.S828L EX14 4.0% missensePE207 MAML3 c.1804A>C p.T602P EX2 11.5% missensePE328 RBM10 c.2074A[2>1] p.K692Rfs*77 EX17 93.3% frameshiftPE330 GALNT12 c.1021G>A p.E341K EX5 14.5% missensePE204 TP53 c.326T>C p.F109S EX4 52.2% missensePE413 HNF1A c.283G>A p.E95K EX1 29.5% missensePE459 APC c.4561G>T p.E1521* EX16E 7.7% nonsensePE485 FLT4 c.2832C>A p.D944E EX20 15.1% missensePE387 EGFR c.2235_2249delGGAATTAAGAGAAGCp.E746_A750del EX19 28.2% cds-del

PE468 EGFR c.2236_2250delGAATTAAGAGAAGCAp.E746_A750del EX19 49.2% cds-delPE536 ARID1B c.220G>A p.E74K EX1 1.2% missensePE558 RBM10 c.2175_2181delCAGTCTCinsAp.S726_L727del EX18 42.3% cds-delPE513 TP53 c.395A>T p.K132M EX5 9.5% missensePE594 ERCC3 c.2294G>A p.R765Q EX15E 31.9% missensePE672 TP53 c.658T>C p.Y220H EX6 6.3% missensePE642 IGF2 c.473C>T p.P158L EX4 13.2% missensePE784 EGFR c.2303G>T p.S768I EX20 36.4% missensePE716 FAT1 c.10748A>C p.D3583A EX19 13.6% missensePE812 MLL3 c.2476G>C p.G826R EX14 9.7% missensePE808 EGFR c.2235_2249delGGAATTAAGAGAAGCp.E746_A750del EX19 12.4% cds-delPE825 FGF6 c.185G>A p.R62H EX1 1.3% missensePE807 EZH2 c.335C>A p.S112Y EX4 1.6% missensePE856 TP53 c.517G>T p.V173L EX5 94.6% missensePE663 LRP1B c.10533G>T p.K3511N EX68 2.2% missensePES121 EGFR c.2369C>T p.T790M EX20 0.76% missensePES121 EGFR c.2235_2249delGGAATTAAGAGAAGCp.E746_A750del EX19 0.42% cds-indelPES121 SPTA1 c.634G>T p.G212W EX5 2.5% missensePES121 EGFR c.2369C>T p.T790M EX20 2.9% missensePES121 CTNNB1 c.1528A>T p.T510S EX10 0.5% missensePES121 FLT4 c.3986G>T p.G1329V EX30 0.4% missensePES130 MPL c.1771T>G p.Y591D EX12 0.3% missensePES130 FCGR2B c.820A>C p.N274H EX7 0.5% missensePES121 PTPN11 c.1492C>T p.R498W EX13 0.6% missensePES121 NTRK3 c.2296T>A p.Y766N EX19 0.2% missensePES121 EGFR c.2369C>T p.T790M EX20 14.9% missensePES121 CDS1 c.288G>A p.M96I EX3 11.1% missensePES121 TP53 c.613T>C p.Y205H EX6 31.4% missensePES121 PSMB5 c.128C>T p.A43V EX1 12.9% missensePES121 EGFR c.2235_2249delGGAATTAAGAGAAGCp.E746_A750del EX19 14.1% cds-indelPES121 MET c.1039G>A p.A347T EX2 12.2% missensePES121 CHEK2 c.298C>T p.Q100* EX2 12.2% nonsensePES121 AQP12A c.83T>G p.L28R EX1 0.5% missensePES121 NTRK1 c.1624G>A p.V542I EX14 6.6% missensePES121 EGFR c.2236_2250delGAATTAAGAGAAGCAp.E746_A750del EX19 25% cds-indelPES121 TP53 c.842A>G p.D281G EX8 12.5% missensePES121 JAK2 c.1849G>T p.V617F EX14 0.5% missensePES121 EGFR c.2369C>T p.T790M EX20 0.2% missensePES121 CCND1 c.839A>T p.E280V EX5 0.1% missensePES121 HGF c.1031T>C p.F344S EX8 0.5% missensePES121 NTRK3 c.905A>T p.Y302F EX9 0.3% missensePES121 APC c.3580T>A p.S1194T EX17 0.5% missensePES121 JAK3 c.2125T>A p.W709R EX16 0.2% missensePES121 BRCA1 c.3694G>T p.G1232C EX10 0.5% missensePES121 APC c.7421C>A p.P2474H EX17 0.5% missensePES121 SETD2 c.2655_2673delTCTTCCACCAGGAATAAAGp.S885Rfs*5 EX3 2.9% frameshiftPES121 NTRK3 c.2371C>T p.R791W EX20 7.2% missensePES121 SETD2 c.7093C>T p.Q2365* EX16 6.9% nonsensePES121 CTNNB1 c.95A>G p.D32G EX3 3.7% missensePES121 CNTN5 c.446G>A p.R149H EX5 4.6% missensePES121 ERBB2 c.1886T>A p.V629D EX20 6.2% missensePES121 OR2T4 c.55A>T p.M19L EX1 0.9% missensePES121 TP53 c.568C>T p.P190S EX6 3% missensePES121 TP53 c.818G>A p.R273H EX8 3.8% missense

PES121 MICALL1 c.195G>T p.L65F EX2 0.8% missensePES121 DDR2 c.1340T>A p.L447Q EX13 0.6% missensePES121 CDH23 c.6275C>A p.T2092K EX48 1.2% missensePES121 TP53 c.451C>T p.P151S EX5 20% missensePE219 RBM10 c.1160C>T p.P387L EX10 8.9% missensePE215 BRD4 c.1957G>A p.E653K EX10 9.7% missensePE424 FUBP1 c.1792C>T p.P598S EX19 1.2% missensePE436 EGFR c.2573T>G p.L858R EX21 9.8% missensePE429 BRIP1 c.830C>T p.S277F EX7 26.9% missensePE607 CSF1R c.2855C[2>1] p.C953Afs*130 EX22E 3.1% frameshiftPE461 ELAC2 c.1349C>T p.A450V EX15 22.8% missensePE417 MLL2 c.5971G>A p.G1991S EX28 18.5% missensePE473 TP53 c.833C>A p.P278H EX8 60.4% missensePE549 NAV3 c.1639G>A p.A547T EX8 5.7% missensePE439 CTNNB1 c.101G>A p.G34E EX3 32.3% missensePE531 MAP2K1 c.371C>T p.P124L EX3 1.1% missensePE587 CTNNB1 c.1349C>T p.T450I EX9 1.6% missensePE508 EZH2 c.1741G>A p.A581T EX15 1.0% missensePE704 APC c.7389A>T p.E2463D EX16E 8.9% missensePE746 OR5L2 c.113C>T p.T38M EX1E 18.1% missensePE690 TET2 c.5134A>T p.R1712* EX11E 27.7% nonsensePE781 SUFU c.1381G>C p.E461Q EX12E 1.8% missensePE814 TP53 c.830G>T p.C277F EX8 1.5% missensePE840 OR5L2 c.55G>A p.D19N EX1E 1.6% missensePE858 ROBO3 c.3859G>T p.A1287S EX26 15.7% missensePE798 MLL3 c.5929C>T p.Q1977* EX36 1.1% nonsensePE884 TSHR c.673T>C p.Y225H EX8 39.1% missensePE890 EGFR c.2582T>A p.L861Q EX21 5.8% missensePE913 LRP1B c.7510G>A p.V2504M EX45 5.5% missensePE270 GRIN2A c.3464G>A p.S1155N EX13 13.3% missensePE223 EPHB6 c.1204C>G p.L402V EX9 1.6% missensePE350 EWSR1 c.1930C[2>1] p.P644Lfs*28 EX17 4.3% frameshiftPE245 PIK3R2 c.1117G>A p.G373R EX10 3.5% missensePE352 DICER1 c.227T>C p.L76P EX4 25.6% missensePE356 NTHL1 c.71G>A p.G24E EX1 17.4% missensePE333 EGFR c.2497T>G p.L833V EX21 1.3% missensePE394 PBRM1 c.285G>A p.M95I EX4 11.0% missensePE447 NOTCH2 c.629C>T p.P210L EX4 1.4% missensePE490 EGFR c.2573T>G p.L858R EX21 11.3% missensePE500 CARD11 c.465G>T p.R155S EX5 5.3% missensePE539 CREBBP c.1723A>G p.S575G EX8 1.6% missensePE593 FAT2 c.3703G>A p.V1235I EX4 17.9% missensePE728 KDR c.3515G>T p.G1172V EX27 4.9% missensePE668 MAGI2 c.1658G>A p.R553Q EX10 7.6% missensePE731 DNAH6 c.1177C>A p.P393T EX7 5.4% missensePE717 PDCD1 c.148G>A p.A50T EX2 1.1% missensePE565 MET c.3029-120C>A . IVS14 0.5% intronPE770 MLL3 c.2011G>C p.E671Q EX14 2.5% missensePE794 ABCA13 c.13538T>G p.V4513G EX51 18.4% missensePE714 DOT1L c.4353_4373delAGGCGGCGCGGCGTCCTCCGCp.G1452_A1458delEX27 2.6% cds-delPE724 TP53 c.841G>A p.D281N EX8 16.0% missensePE742 IDH2 c.155A>C p.E52A EX2 13.1% missensePE785 OR4A15 c.593G>A p.G198E EX1E 45.2% missensePE656 EGFR c.2235_2249delGGAATTAAGAGAAGCp.E746_A750del EX19 3.8% cds-del

PE891 IDH2 c.770C>T p.A257V EX6 4.0% missensePE895 TSC2 c.4022G>T p.S1341I EX34 1.0% missensePE341 FLG c.8606G>C p.R2869T EX3 1.8% missensePE402 CDC73 c.1036C>G p.Q346E EX12 1.4% missensePE400 MLL2 c.7838C>T p.P2613L EX31 11.8% missensePE424 MLL2 c.6241G>A p.E2081K EX31 1.0% missensePE364 SETD2 c.1466C>G p.S489C EX3 27.7% missensePE457 HGF c.892G>C p.V298L EX8 4.3% missensePE547 NF1 c.8396A>T p.Q2799L EX57E 5.0% missensePE474 ABCC2 c.3542G>A p.R1181Q EX25 1.1% missensePE588 PALB2 c.2017G>T p.E673* EX5 1.3% nonsensePE553 MS4A1 c.573+2T>G . IVS6 26.5% splice-5PE689 RAD50 c.3025G>C p.D1009H EX19 2.2% missensePE729 TGFBR2 c.1405C>T p.Q469* EX6 0.5% nonsensePE740 KIT c.999G>C p.L333F EX6 1.4% missensePE755 NTRK1 c.2326G>T p.D776Y EX17E 10.8% missensePE799 SNX29 c.854C>T p.T285I EX8 8.0% missensePE678 TOP1 c.1570G>A p.V524M EX15 1.7% missensePE877 ATM c.8010+1G>T . IVS54 8.2% splice-5PE816 ALK c.3202G>A p.A1068T EX20 11.5% missensePE258 TP53 c.404G>T p.C135F EX5 1.3% missensePE296 EGFR c.2573T>G p.L858R EX21 1.6% missensePE319 COL5A3 c.849+1G>A . IVS6 15.0% splice-5PE376 SLC16A1 c.1493G>C p.S498T EX5 13.1% missensePE470 IFITM3 c.160G>A p.V54M EX1 1.6% missensePE457 PDGFRA c.2896C>T p.H966Y EX22 3.5% missensePE523 MLL2 c.8173A>T p.S2725C EX32 6.3% missensePE482 ATRX c.5257A>C p.N1753H EX20 31.1% missensePE548 EP300 c.1627A>G p.M543V EX8 20.7% missensePE547 CDH23 c.2032G>A p.V678I EX19 6.3% missensePE421 TOP1 c.274G>A p.E92K EX4 7.9% missensePE512 TOP1 c.418C>T p.R140* EX6 11.6% nonsensePE588 PTEN c.389G>A p.R130Q EX5 54.9% missensePE553 LRP1B c.5915A>T p.H1972L EX37 15.4% missensePE440 HNF1A c.279C>G p.S93R EX1 7.0% missensePE678 MLL2 c.8470A>G p.T2824A EX34 5.0% missensePE748 SMAD2 c.1198C>G p.Q400E EX10 0.6% missensePE877 CARD11 c.2840-1G>C . IVS21 2.3% splice-3PE826 FLCN c.619G>A p.V207M EX7 7.0% missensePE929 NBN c.1520ATCT[2>1] p.S509Rfs*19 EX11 1.0% frameshiftPE821 TBX3 c.604G>A p.V202I EX2 2.5% missensePE632 SDHAF2 c.46C>G p.L16V EX2 1.1% missensePE931 WT1 c.1390G>A p.D464N EX9 4.4% missensePE270 TGFBR1 c.214A>G p.I72V EX2 22.9% missensePE274 FAT1 c.8959G>A p.E2987K EX11 6.4% missensePE252 CTNNB1 c.1004A>T p.K335I EX7 1.2% missensePE386 SETD2 c.3901C>T p.Q1301* EX3 5.8% nonsensePE364 FLT1 c.3225C>G p.I1075M EX24 1.2% missensePE370 TP53 c.498dupA p.Q167Tfs*14 EX5 0.5% frameshiftPE418 CSMD3 c.11000T>C p.V3667A EX71E 6.1% missensePE463 SMAD4 c.1590_1601delCCGGGCCCTCCAp.H530_L533del EX12E 18.0% cds-delPE503 SETD2 c.323T>G p.L108R EX3 1.8% missensePE544 EGFR c.2573T>G p.L858R EX21 84.7% missensePE733 TRIM24 c.406GA[3>1] p.E136Dfs*4 EX2 4.0% frameshift

PE705 EGFR c.2497T>G p.L833V EX21 52.3% missensePE689 MLL c.2671G>C p.E891Q EX3 1.2% missensePE479 EGFR c.2369C>T p.T790M EX20 11.0% missensePE478 STK11 c.465-1G>T . IVS3 8.4% splice-3PE757 BRAF c.1781A>G p.D594G EX15 49.3% missensePE739 TP53 c.1023C[2>1] p.R342Efs*3 EX10 23.3% frameshiftPE735 EGFR c.2573T>G p.L858R EX21 87.9% missensePE892 BLM c.938C>G p.S313C EX4 9.3% missensePE893 TP53 c.517G>A p.V173M EX5 68.2% missensePE897 AXL c.26G>T p.G9V EX1 1.1% missensePE925 OR4C6 c.258G>T p.K86N EX1E 18.4% missensePE297 GNAS c.13G>C p.G5R EX1 6.6% missensePE309 ASMTL c.1219G>A p.G407R EX9 31.1% missensePE207 WWP2 c.1379G>A p.R460Q EX14 19.6% missensePE356 EP300 c.6142C>G p.Q2048E EX31 14.9% missensePE428 PDGFRB c.635C>T p.S212L EX5 5.1% missensePE546 PTPRD c.4945A>T p.K1649* EX40 1.7% nonsensePE214 KIT c.1369G>T p.V457L EX9 19.1% missensePE593 ARID1A c.5311C>T p.P1771S EX20E 5.5% missensePE664 LRP1B c.12523_12524delCCinsATp.P4175I EX82 9.4% missensePE590 EZH2 c.9G>T p.Q3H EX2 0.5% missensePE654 NCOR1 c.1378G>T p.G460* EX13 71.4% nonsensePE701 ABL2 c.236C>A p.P79H EX3 25.5% missensePE649 CEBPA c.72C>G p.H24Q EX1E 7.0% missensePE564 MS4A1 c.855T>A p.D285E EX8E 21.6% missensePE831 EGFR c.2253_2276delATCTCCGAAAGCCAACAAGGAAATp.S752_I759del EX19 12.0% cds-delPE792 SETD2 c.6224_6241delGGAAACGAAGAAGCTCCCinsTp.R2075Ifs*9 EX14 2.3% frameshiftPE863 PARK2 c.3G>T p.0? EX1 1.1% init-lossPE738 EGFR c.2573T>G p.L858R EX21 2.6% missensePE583 ALK c.3607G>A p.D1203N EX23 6.0% missensePE876 CYP3A4 c.1335G>T p.M445I EX12 0.7% missensePE900 NTRK2 c.208G>A p.E70K EX4 1.3% missensePE899 FANCA c.897C>G p.F299L EX11 1.7% missensePE854 CTNNB1 c.110C>T p.S37F EX3 1.0% missensePE270 KDM5C c.704A>G p.K235R EX6 34.0% missensePE274 SMARCA4 c.3547G>A p.D1183N EX26 6.5% missensePE252 FLG c.8582C>T p.A2861V EX3 24.3% missensePE331 TP53 c.769C>G p.L257V EX7 8.3% missensePE463 EGFR c.2240_2257delTAAGAGAAGCAACATCTCp.L747_P753delinsSEX19 11.4% cds-indelPE582 MAD1L1 c.1969G>A p.E657K EX18 9.8% missensePE411 SLC16A1 c.1420C>A p.P474T EX5E 17.6% missensePE581 KDM5C c.618C>G p.F206L EX5 95.6% missensePE551 RBM10 c.2413G>T p.E805* EX20 16.7% nonsensePE630 PTEN c.379G>A p.G127R EX5 72.2% missensePE733 SLC34A2 c.2003_2004delTAp.I668Nfs*3 EX13E 28.1% frameshiftPE669 NTRK3 c.1219T>A p.F407I EX11 0.5% missensePE755 TP53 c.730G>A p.G244S EX7 39.5% missensePE793 KEAP1 c.1132G>T p.G378C EX3 23.9% missensePE768 FAT2 c.2621A[2>1] p.E874Dfs*38 EX1 6.9% frameshiftPE857 EGFR c.2369C>T p.T790M EX20 75.3% missensePE878 FAT1 c.12614T>A p.M4205K EX25 2.7% missensePE753 MLL2 c.13039C>T p.Q4347* EX39 20.1% nonsensePE703 PAX5 c.47-1G>T . IVS1 28.0% splice-3PE871 FGFR2 c.1678C>A p.L560I EX13 0.4% missense

PE892 CDH23 c.4G>A p.G2R EX2 5.4% missensePE893 NBN c.1442C>T p.A481V EX11 26.2% missensePE925 EGFR c.2240_2254delTAAGAGAAGCAACATp.L747_T751del EX19 52.3% cds-delPE223 U2AF1 c.101C>T p.S34F EX2 11.7% missensePE283 CDKN2A c.322G>T p.D108Y EX2 0.5% missensePE309 EPHA7 c.2615G>T p.C872F EX15 26.1% missensePE207 BRCA1 c.101C>A p.P34H EX3 18.7% missensePE348 NOTCH1 c.414_415delCCinsAGp.C138* EX4 4.4% stop-gainPE503 MYC c.329C>A p.T110K EX2 3.1% missensePE546 KRAS c.35G>T p.G12V EX2 2.6% missensePE214 EGFR c.2236_2250delGAATTAAGAGAAGCAp.E746_A750del EX19 44.0% cds-delPE593 MITF c.1444G>A p.D482N EX10E 3.5% missensePE712 EGFR c.2235_2249delGGAATTAAGAGAAGCp.E746_A750del EX19 9.2% cds-delPE728 TP53 c.451C[5>4] p.P152Rfs*18 EX5 5.0% frameshiftPE730 EGFR c.2573T>G p.L858R EX21 7.2% missensePE760 TP53 c.326_330delTCCGTp.F109Sfs*38 EX4 77.7% frameshiftPE694 SETD2 c.4871C>T p.S1624F EX7 35.8% missensePE788 CREBBP c.4449C>G p.I1483M EX27 6.9% missensePE745 EGFR c.2573T>G p.L858R EX21 75.3% missensePE679 NOTCH2 c.298G>T p.D100Y EX3 9.9% missensePE792 FAT2 c.5178C>A p.Y1726* EX9 1.0% nonsensePE738 MYC c.292C>T p.R98W EX2 1.0% missensePE856 PIK3CA c.1624G>A p.E542K EX10 7.5% missensePE876 GAB2 c.1987G>C p.D663H EX10E 2.0% missensePE773 PIK3CA c.1633G>A p.E545K EX10 2.0% missensePE854 RB1 c.1608C>G p.I536M EX17 1.4% missensePE309 GRIN2A c.1122+1G>T . IVS4 25.0% splice-5PE207 ROS1 c.3017A>G p.E1006G EX20 18.4% missensePE348 LRP1B c.4866G>A p.W1622* EX29 3.1% nonsensePE356 ATRX c.3337G>A p.E1113K EX9 43.0% missensePE399 TP53 c.818G>T p.R273L EX8 4.6% missensePE546 FLT1 c.3066C>A p.D1022E EX23 1.9% missensePES121 KRAS c.35G>A p.G12D EX2 59.5% missensePE214 MET c.3396A>T p.K1132N EX17 7.7% missensePE664 PIK3CG c.2985G>T p.M995I EX10 10.8% missensePE672 GRIN2A c.2537G>A p.R846H EX12 3.1% missensePE654 SERPINB4 c.639G>A p.M213I EX7 36.2% missensePE670 TP53 c.469G>T p.V157F EX5 2.3% missensePE760 RPTOR c.2362C>T p.R788C EX20 1.5% missensePE786 PMS2 c.-87-u382C>A . . 2.8% promoterPE792 BRAF c.1799T>A p.V600E EX15 3.6% missensePE800 RBM10 c.2362-2A>G . IVS19 17.2% splice-3PE738 TGFBR1 c.1014T>A p.N338K EX6 1.8% missensePE876 MLL c.2672A>C p.E891A EX3 4.0% missensePE854 TP53 c.600T[3>4] p.L201Ffs*8 EX6 2.2% frameshiftPE321 TET2 c.3782G>C p.R1261P EX6 0.8% missensePE436 PTCH1 c.3239C>T p.A1080V EX19 4.5% missensePE417 BRIP1 c.573G>T p.K191N EX6 52.8% missensePE473 AR c.2424G>A p.M808I EX6 16.0% missensePE439 EGFR c.2237_2255delAATTAAGAGAAGCAACATCinsTp.E746_S752delinsVEX19 17.6% cds-indelPE528 EPHB4 c.2492A>G p.N831S EX15 9.0% missensePE639 TP53 c.112C>T p.Q38* EX4 2.8% nonsensePE508 FAM123B c.1070A>T p.K357M EX2E 1.2% missensePE613 MLL3 c.13199G>A p.R4400Q EX52 4.4% missense

PE704 ZFHX3 c.5253_5255delAGTinsGGCp.V1752A EX9 5.9% missensePE746 CREBBP c.6229C>A p.P2077T EX31E 26.1% missensePE755 KIT c.1901G>A p.R634Q EX13 13.0% missensePE677 TP53 c.404G>T p.C135F EX5 7.8% missensePE840 ATM c.1937C>T p.S646L EX13 5.5% missensePE774 RARA c.1201G>C p.E401Q EX9E 1.3% missensePE858 GRIN2A c.3541A[2>3] p.N1181Kfs*5 EX13E 5.6% frameshiftPE801 MET c.3028G>C p.D1010H EX14 54.2% missensePE890 ACIN1 c.3718G>C p.E1240Q EX19E 1.0% missensePE917 TGFBR2 c.1404_1405insT p.Q469Sfs*19 EX6 21.9% frameshiftPES121 SETD1B c.125A>G p.K42R 1% missensePE423 EGFR c.2310_2311insGGGp.D770_N771insGEX20 5.3% cds-insPE436 NOTCH1 c.3473C>G p.A1158G EX21 1.5% missensePE374 EGFR c.2573T>G p.L858R EX21 3.8% missensePE472 POT1 c.975G>T p.E325D EX12 11.0% missensePE607 SERPINB4 c.636G>T p.M212I EX7 2.1% missensePE417 AR c.176A>T p.Q59L EX1 13.0% missensePE528 MLL3 c.9203dupA p.Q3069Afs*18 EX38 8.7% frameshiftPE572 FLT1 c.1334C>A p.P445Q EX10 43.9% missensePE704 TP53 c.631A>G p.T211A EX6 13.2% missensePE666 EGFR c.2369C>T p.T790M EX20 36.5% missensePE758 BRAF c.1781A>G p.D594G EX15 31.5% missensePE677 NF1 c.1400_1401delCAp.T467Ifs*2 EX13 10.2% frameshiftPE858 GRIN2A c.1692G>T p.M564I EX8 15.0% missensePE703 LRP1B c.13439C>A p.P4480H EX89 38.3% missensePE801 FGFR1 c.378G>T p.E126D EX4 16.2% missensePE801 KRAS c.35G>A p.G12D EX2 32.0% missensePE917 HGF c.533G>A p.R178Q EX5 3.6% missensePE341 CASP8 c.457G>A p.G153S EX2 13.0% missensePE433 LRP1B c.9040G>C p.E3014Q EX57 46.4% missensePE424 MLL2 c.1021G>A p.E341K EX8 1.2% missensePE364 FOXP1 c.1351G>A p.D451N EX12 40.3% missensePE457 GRM3 c.371A>T p.E124V EX2 4.8% missensePE547 SRSF2 c.284C>A p.P95H EX1 3.4% missensePE588 TP53 c.380C>T p.S127F EX5 71.2% missensePE440 MLH3 c.2014A[8>9] p.N674Kfs*13 EX2 11.4% frameshiftPE689 CTNNA1 c.398C>G p.S133C EX4 2.6% missensePE729 ZMAT3 c.394G>C p.G132R EX4 1.1% missensePE740 FAT1 c.12052G>A p.A4018T EX22 1.3% missensePE780 EGFR c.2236_2250delGAATTAAGAGAAGCAp.E746_A750del EX19 14.2% cds-delPE799 TP53 c.701A>G p.Y234C EX7 33.7% missensePE678 GNAS c.895G>A p.E299K EX11 1.8% missensePE793 ARID1A c.4087C>T p.Q1363* EX17 19.2% nonsensePE563 DNMT3A c.1825T>C p.F609L EX15 51.5% missensePE878 ARID1A c.1429C>G p.H477D EX3 3.6% missensePE877 ZBTB16 c.931G>C p.E311Q EX2 2.5% missensePE911 BRCA1 c.1511G>A p.R504H EX10 1.4% missensePE903 TP53 c.734G>A p.G245D EX7 11.9% missensePE816 LRP1B c.3496G>T p.G1166C EX22 1.0% missensePE220 EGFR c.2573T>G p.L858R EX21 34.4% missensePE222 TP53 c.96+1G>C . IVS3 30.5% splice-5PE207 KDM5A c.1820G>A p.R607H EX14 2.0% missensePE353 FLCN c.889G>A p.E297K EX9 5.4% missensePE338 FAM131B c.883G>C p.D295H EX7 12.3% missense

PE363 TTL c.519A>G p.I173M EX4 38.5% missensePE429 ROS1 c.6094G>A p.G2032R EX38 4.1% missensePE417 MYCN c.899C>A p.T300N EX3E 28.3% missensePE555 SETD2 c.6325C>T p.R2109* EX15 2.9% nonsensePE594 ZFHX3 c.10518CGG[5>3] p.G3507[6>4] EX10E 13.3% cds-delPE581 HIST1H2BDc.263C>T p.S88L EX1E 25.0% missensePE572 LRP1B c.12827C>A p.A4276E EX84 44.4% missensePE690 EPHA2 c.1456C>T p.R486C EX7 3.2% missensePE480 TRIM58 c.580G>T p.A194S EX3 6.4% missensePE437 BCOR c.2786C>T p.A929V EX4 15.1% missensePE805 TET2 c.5079C>G p.Y1693* EX11E 0.5% nonsensePE749 CASP8 c.1176C>G p.I392M EX8 3.9% missensePE849 SERPINB3 c.899G>A p.R300K EX8E 1.0% missensePE850 RBM10 c.1894C[3>4] p.D633Rfs*9 EX16 35.0% frameshiftPE863 XIAP c.1192C>T p.Q398* EX6 30.0% nonsensePE875 CIC c.612G>A p.M204I EX5 11.3% missensePE828 HRAS c.37G>C p.G13R EX2 45.5% missensePE218 TP53 c.817C>T p.R273C EX8 1.8% missensePE302 TP53 c.716A>G p.N239S EX7 92.1% missensePE319 PASK c.314T>C p.V105A EX3 6.3% missensePE310 TP53 c.740A>T p.N247I EX7 6.8% missensePE391 TP53 c.742C>T p.R248W EX7 35.9% missensePE457 MTOR c.3676G>A p.E1226K EX25 6.5% missensePE523 ABCC2 c.3514G>C p.V1172L EX25 21.4% missensePES121 TP53 c.503A>C p.H168P EX5 5.5% missensePE421 ARID2 c.3943G>T p.E1315* EX15 16.2% nonsensePE588 PBRM1 c.1814C>G p.S605C EX15 1.6% missensePE440 PDGFRB c.732C>A p.N244K EX5 25.0% missensePE671 RB1 c.2520+1_2520+4delGTGA. IVS24 17.1% splice-5PE689 LRP1B c.6302G>A p.R2101K EX39 2.2% missensePE650 TP53 c.743G>A p.R248Q EX7 1.3% missensePE678 MSH6 c.550A>C p.N184H EX3 3.1% missensePE812 EGFR c.2573T>G p.L858R EX21 17.0% missensePE873 FLG c.8513G>A p.S2838N EX3E 2.3% missensePE877 MYCN c.834C>G p.D278E EX3E 1.0% missensePE911 NTRK3 c.388C>G p.R130G EX5 1.8% missensePE916 MED12 c.6045-1G>A . IVS41 13.9% splice-3PE591 STK11 c.580G>T p.D194Y EX4 42.1% missensePE821 DNMT3A c.1014+1delG . IVS8 6.5% splice-5PE833 ZFHX3 c.9591GCA[7>9] p.Q3197[8>10] EX10E 3.7% cds-insPE270 PDGFRA c.646C>A p.L216I EX5 36.9% missensePE274 FAT1 c.8626G>A p.D2876N EX10 6.6% missensePE257 TP53 c.404G>T p.C135F EX5 18.7% missensePE252 ERBB3 c.2783A>G p.E928G EX23 33.6% missensePE349 ASXL1 c.3163A>G p.R1055G EX13 5.4% missensePE331 PTCH1 c.2222C>T p.A741V EX14 1.8% missensePE424 FLCN c.247G>A p.E83K EX4 1.1% missensePE364 WT1 c.330_331delCCinsTTp.P111S EX1 12.5% missensePE411 MYCL1 c.552G>T p.Q184H EX2 18.0% missensePE581 RBM10 c.1585A>T p.K529* EX13 95.9% nonsensePE510 TP53 c.76delC p.L26Ffs*18 EX3 30.7% frameshiftPE620 FANCD2 c.1938A[4>3] p.A648Pfs*18 EX21 17.2% frameshiftPE514 FANCA c.1053_1054delACinsTTp.H352Y EX12 11.3% missensePE733 ATR c.4006G>C p.V1336L EX22 8.1% missense

PE669 RB1 c.652T>G p.L218V EX7 10.3% missensePE689 CDKN2A c.383G>C p.R128P EX2 1.5% missensePE793 TP53 c.746G>A p.R249K EX7 9.6% missensePE768 FAT2 c.9260C>T p.A3087V EX12 5.7% missensePE703 PTPRD c.1092G>T p.E364D EX21 27.9% missensePE739 OR4A15 c.556G>A p.V186I EX1E 11.9% missensePE869 MSH6 c.1595C>G p.S532C EX4 2.1% missensePE919 EGFR c.2235_2249delGGAATTAAGAGAAGCp.E746_A750del EX19 1.2% cds-delPE816 RECQL4 c.1390G>A p.E464K EX7 1.9% missensePE343 BCOR c.8C>T p.S3L EX2 1.0% missensePE438 ARID1A c.2956G>T p.D986Y EX10 3.9% missensePE472 CBL c.1211G>A p.C404Y EX8 1.2% missensePE520 SETD2 c.323T>G p.L108R EX3 10.5% missensePE587 PTEN c.733C>T p.Q245* EX7 7.5% nonsensePE737 RET c.2179G>A p.G727R EX12 29.0% missensePE622 MAP2K2 c.158G>T p.R53L EX2 1.5% missensePE471 MCL1 c.8G>C p.G3A EX1 3.8% missensePE741 EGFR c.2236_2250delGAATTAAGAGAAGCAp.E746_A750del EX19 8.6% cds-delPE687 HCLS1 c.884C>T p.P295L EX10 1.7% missensePE848 DNMT3A c.2645G>A p.R882H EX23E 2.1% missensePE816 NTRK1 c.2201C>A p.T734K EX16 1.5% missensePE491 MLL2 c.11050C>T p.Q3684* EX39 73.6% nonsensePE909 PDE4DIP c.6535C>T p.H2179Y EX40 3.9% missensePE913 PTCH1 c.2107G>T p.E703* EX14 19.2% nonsensePE285 ARID1A c.5372C>T p.S1791L EX20 0.8% missensePE309 MAP3K1 c.3143A>T p.Q1048L EX14 12.6% missensePE207 ERBB4 c.1342C>T p.Q448* EX12 18.4% nonsensePE204 ARID1A c.4005-1G>C . IVS16 10.4% splice-3PE416 ALK c.4456C>T p.P1486S EX29E 0.7% missensePE453 EGFR c.2240_2254delTAAGAGAAGCAACATp.L747_T751del EX19 10.6% cds-delPE546 TP53 c.857A>G p.E286G EX8 1.4% missensePES121 KRAS c.35G>A p.G12D EX2 54.2% missensePE214 JAK2 c.3300G>A p.M1100I EX25E 8.5% missensePE664 POLE c.6071C>T p.P2024L EX44 12.4% missensePE637 EGFR c.2235_2249delGGAATTAAGAGAAGCp.E746_A750del EX19 0.4% cds-delPE701 EPHA3 c.58G>A p.G20R EX1 33.4% missensePE659 NCOR1 c.1661T>A p.I554K EX16 0.7% missensePE760 PCK1 c.1807G>A p.D603N EX10E 7.4% missensePE385 EP300 c.4166A>T p.N1389I EX25 1.0% missensePE788 RPS6KB1 c.214T>G p.F72V EX3 1.6% missensePE745 TP53 c.722C>T p.S241F EX7 42.5% missensePE679 DDR2 c.2312G>T p.W771L EX17 11.0% missensePE564 TP53 c.375+1G>T . IVS4 49.7% splice-5PE858 DNMT3A c.1904G>A p.R635Q EX16 1.4% missensePE276 EGFR c.2236_2250delGAATTAAGAGAAGCAp.E746_A750del EX19 0.6% cds-delPE322 PDGFRA c.2156+1G>T . IVS15 73.1% splice-5PE436 KEL c.259G>A p.D87N EX4 12.3% missensePE429 ARAF c.1130G>A p.G377E EX11 23.6% missensePE374 DNMT3A c.1609T>C p.C537R EX14 3.9% missensePE472 MET c.1216T>G p.S406A EX3 2.7% missensePE467 EPHA3 c.274G>T p.A92S EX3 3.7% missensePE607 EGFR c.2573T>G p.L858R EX21 21.0% missensePE417 TP53 c.725G>C p.C242S EX7 73.6% missensePE397 EGFR c.2236_2250delGAATTAAGAGAAGCAp.E746_A750del EX19 6.4% cds-del

PE704 HSP90AA1 c.1087A>G p.K363E EX6 4.5% missensePE666 APC c.1993_1999delTTATTACp.L665Nfs*3 EX16E 48.9% frameshiftPE758 TP73 c.1695C>A p.N565K EX14E 21.5% missensePE781 RBM10 c.1284_1285delGCinsTTp.Q429* EX11 19.7% stop-gainPE769 ARAP3 c.1537G>A p.D513N EX10 1.0% missensePE677 ROBO3 c.217C>G p.L73V EX2 7.5% missensePE858 FANCM c.1247T>A p.L416Q EX7 2.8% missensePE884 TP53 c.488A>G p.Y163C EX5 79.4% missensePE801 IRF2 c.863T>G p.L288R EX9E 26.5% missensePE913 EPHA3 c.1782G>T p.R594S EX10 15.3% missensePE917 CUL3 c.265G>A p.V89M EX3 18.7% missensePE296 FAT2 c.778G>A p.V260M EX1 1.5% missensePE376 EGFR c.2235_2249delGGAATTAAGAGAAGCp.E746_A750del EX19 3.4% cds-delPE429 KDR c.1562C>T p.A521V EX12 1.8% missensePE364 LRP1B c.445G>T p.D149Y EX4 40.2% missensePE448 TNFAIP3 c.1087G>A p.G363R EX7 1.2% missensePE457 EPHA5 c.2836G>T p.V946F EX16 2.1% missensePE523 MYCN c.539C>G p.A180G EX2 4.9% missensePE421 CRKL c.841G>A p.G281R EX3E 1.1% missensePE511 EGFR c.2573T>G p.L858R EX21 79.9% missensePE553 PIK3CA c.1633G>A p.E545K EX10 9.7% missensePE440 RB1 c.2049_2063delTTTCCAGCACACCCTp.F684_L688del EX20 23.0% cds-delPE502 KRAS c.35G>C p.G12A EX2 1.6% missensePE689 ATP11B c.1502G>C p.S501T EX14 1.1% missensePE678 GRIN2A c.1574C>G p.S525C EX7 3.5% missensePE863 EPHA3 c.1607C>T p.S536F EX8 1.0% missensePE873 EGFR c.2235_2249delGGAATTAAGAGAAGCp.E746_A750del EX19 0.2% cds-delPE911 FLCN c.247G>A p.E83K EX4 1.2% missensePE910 TERT c.-58-u88C>T . . 3.6% promoterPE632 MLL c.7601A>T p.N2534I EX27 1.5% missensePE903 THBS2 c.2622C>A p.D874E EX18 7.9% missensePE270 EGFR c.2573T>G p.L858R EX21 20.7% missensePE223 FLT4 c.3967G>A p.E1323K EX30 1.2% missensePE251 SMARCA4 c.4735G>T p.E1579* EX33 0.5% nonsensePE350 TP53 c.126T[3>2] p.L43*fs*1 EX4 12.9% frameshiftPE245 CDK12 c.2374A>G p.I792V EX5 14.9% missensePE352 MED12 c.6067C>T p.Q2023* EX42 42.6% nonsensePE356 ACVR1B c.349G>C p.E117Q EX3 19.6% missensePE333 ERBB4 c.1541G>A p.G514E EX13 0.9% missensePE378 NF2 c.743A>G p.N248S EX8 3.9% missensePE460 FANCM c.5811C>G p.C1937W EX22 8.4% missensePE539 DICER1 c.5701A[3>4] p.S1902Ifs*14 EX28E 12.4% frameshiftPE594 ATRX c.2954G>C p.S985T EX9 2.2% missensePE593 AFF3 c.1856_1862delCCTCAGCinsGp.T619_A621delinsSEX14 20.8% cds-indelPE717 TP73 c.462G>C p.Q154H EX5 3.8% missensePE708 SLX4 c.4773G>A p.M1591I EX14 1.9% missensePE754 EGFR c.2314_2319dupCCCCACp.P772_H773dup EX20 1.1% cds-insPE840 RAF1 c.776C>T p.S259F EX7 19.7% missensePE915 RBM10 c.1978C>T p.Q660* EX16 35.8% nonsensePE656 HIST1H1C c.603G>C p.K201N EX1E 0.8% missensePE891 HNF1A c.1879G>A p.A627T EX10E 1.9% missensePE935 KRAS c.35G>A p.G12D EX2 22.4% missensePE209 EGFR c.2236_2250delGAATTAAGAGAAGCAp.E746_A750del EX19 0.4% cds-delPE303 TP53 c.716A>G p.N239S EX7 3.2% missense

PE207 SF3B1 c.2015C>A p.A672D EX14 10.9% missensePE328 FLT3 c.1594C>G p.P532A EX12 34.9% missensePE204 EP300 c.6474G>T p.M2158I EX31 12.0% missensePE390 PDK1 c.527A>G p.N176S EX4 59.6% missensePE367 POT1 c.1211G>A p.G404D EX14 0.9% missensePE485 FAM135B c.4144G>T p.A1382S EX20E 14.7% missensePE468 EGFR c.2369C>T p.T790M EX20 32.2% missensePES130 EGFR c.2236_2250delGAATTAAGAGAAGCAp.E746_A750del EX19 34.2% cds-delPE594 BARD1 c.1518_1519delTGinsCAp.V507M EX6 70.5% missensePE561 EGFR c.2573T>G p.L858R EX21 46.0% missensePE784 EPHB6 c.911G>T p.G304V EX7 14.4% missensePE716 TP53 c.659A>G p.Y220C EX6 12.4% missensePE679 HIST1H1C c.447G>C p.K149N EX1E 9.8% missensePE747 EGFR c.2582T>A p.L861Q EX21 1.3% missensePE743 SRSF7 c.361C>T p.R121C EX3 17.7% missensePE808 TP53 c.991C>T p.Q331* EX9 13.0% nonsensePE863 MLL2 c.8047C>T p.R2683C EX32 3.6% missensePE807 ABL1 c.1164G>C p.M388I EX7 1.2% missensePE653 NOTCH4 c.2056C>T p.P686S EX13 35.1% missensePE901 BMPR1A c.849G>A p.M283I EX9 4.4% missensePE250 TET2 c.3500+1G>T . IVS4 4.0% splice-5PE297 TP53 c.395A>G p.K132R EX5 33.0% missensePE276 ATM c.2494C>T p.R832C EX17 13.2% missensePE243 EGFR c.2369C>T p.T790M EX20 24.8% missensePE207 EPAS1 c.1757A>G p.D586G EX12 21.1% missensePE356 POLD1 c.3025C>T p.R1009C EX24 19.4% missensePE382 EGFR c.2235_2249delGGAATTAAGAGAAGCp.E746_A750del EX19 3.8% cds-delPE394 SLX4 c.2476G>A p.E826K EX12 12.7% missensePE500 STAG2 c.2027G>T p.G676V EX21 10.9% missensePE407 DNMT3A c.2245delC p.R749Afs*30 EX19 0.9% frameshiftPE527 ZFHX3 c.247G>T p.E83* EX2 21.3% nonsensePE507 TP53 c.329G>T p.R110L EX4 47.9% missensePE318 CDK13 c.1097G>A p.R366H EX1 0.5% missensePE593 JAK2 c.1214C>T p.S405L EX9 12.1% missensePE654 FLI1 c.1253C[2>1] p.A419Hfs*30 EX9E 76.6% frameshiftPE712 POM121L12c.563G>A p.G188E EX1E 14.1% missensePE673 RBM10 c.630C[2>1] p.R211Vfs*16 EX5 58.9% frameshiftPE713 MLL2 c.2695delG p.A899Pfs*31 EX10 7.3% frameshiftPE674 EGFR c.2240_2254delTAAGAGAAGCAACATp.L747_T751del EX19 37.4% cds-delPE709 EGFR c.2235_2249delGGAATTAAGAGAAGCp.E746_A750del EX19 7.6% cds-delPE767 RBM10 c.771G>T p.Q257H EX6 3.2% missensePE717 RBM10 c.813C>A p.Y271* EX7 4.0% nonsensePE618 MAF c.544C>T p.H182Y EX1E 0.5% missensePE795 TP53 c.672+2T>C . IVS6 11.9% splice-5PE791 CHEK2 c.538C>T p.R180C EX4 0.6% missensePE685 EGFR c.2239_2251delTTAAGAGAAGCAAinsCp.L747_T751delinsPEX19 0.3% cds-indelPE800 PARP1 c.1996C>A p.P666T EX14 1.1% missensePE863 FAT1 c.10891A>G p.I3631V EX19 2.3% missensePE309 MAGOH c.407T[2>1] p.I137Lfs*24 EX5 22.8% frameshiftPE207 AKT1 c.1363+1G>A . IVS13 18.4% splice-5PE348 HGF c.1940G>C p.R647P EX17 2.5% missensePE503 MLL c.4589G>T p.C1530F EX13 6.7% missensePE405 EGFR c.2573T>G p.L858R EX21 0.5% missensePE546 WDR90 c.4385G>A p.W1462* EX35 1.4% nonsense

PES121 TP53 c.844C>T p.R282W EX8 63.9% missensePE664 ATM c.6976-1G>A . IVS47 5.7% splice-3PE637 EPHA3 c.1234G>A p.V412I EX5 0.7% missensePE654 SMARCA4 c.915delG p.Q306Sfs*20 EX6 71.1% frameshiftPE732 CBLB c.1757C>T p.P586L EX12 5.5% missensePE760 SMAD4 c.1598T>G p.L533R EX12E 92.0% missensePE788 TP53 c.746G>C p.R249T EX7 11.5% missensePE745 SH2B3 c.62C>A p.A21E EX2 17.7% missensePE786 EGFR c.2573T>G p.L858R EX21 13.4% missensePE792 SLX4 c.929G>A p.R310Q EX4 2.8% missensePE876 HNF1A c.955G>A p.G319S EX4 1.4% missensePE773 EGFR c.2573T>G p.L858R EX21 3.6% missensePE210 EGFR c.2235_2249delGGAATTAAGAGAAGCp.E746_A750del EX19 52.3% cds-delPE267 IL7R c.835C>A p.H279N EX7 20.0% missensePE260 NPM1 c.258G[2>1] p.V87Ffs*8 EX3 0.7% frameshiftPE263 IKZF1 c.146G>T p.S49I EX3 19.7% missensePE345 TP53 c.524G>A p.R175H EX5 55.1% missensePE207 NOTCH4 c.1222A>C p.S408R EX7 6.0% missensePE279 DNMT3A c.2192T>A p.F731Y EX19 1.4% missensePE606 TET2 c.755_756delTTinsAp.I252Kfs*41 EX3 1.2% frameshiftPE339 CDH23 c.4705G>A p.E1569K EX38 2.8% missensePE320 CDH11 c.1040T>A p.V347E EX8 22.0% missensePE379 MET c.2207T>G p.F736C EX9 14.6% missensePE429 ARID1A c.1519C>T p.Q507* EX3 17.5% nonsensePE451 TP53 c.451C>T p.P151S EX5 3.1% missensePE454 POT1 c.1676T>A p.L559H EX17 97.0% missensePE594 TYRP1 c.785C>T p.T262M EX4 13.3% missensePE764 TP53 c.517G>A p.V173M EX5 56.4% missensePE684 MET c.3682G>C p.D1228H EX19 28.4% missensePE772 RBM10 c.2537C>T p.S846L EX20 36.9% missensePE823 EGFR c.2235_2249delGGAATTAAGAGAAGCp.E746_A750del EX19 0.4% cds-delPE855 EGFR c.2573T>G p.L858R EX21 77.4% missensePE491 JAK1 c.907C>T p.H303Y EX7 22.2% missensePE898 PTEN c.254T>C p.V85A EX5 43.4% missensePE875 MLL c.299C>G p.S100C EX1 2.2% missensePE631 ERRFI1 c.920C>G p.S307W EX4E 1.0% missensePE894 EGFR c.2573T>G p.L858R EX21 8.3% missensePE828 LRP1B c.3842G>T p.S1281I EX24 77.9% missensePE292 BRAF c.1799T>A p.V600E EX15 4.0% missensePE293 CARD11 c.2062C>T p.R688* EX16 86.2% nonsensePE362 LRP1B c.3518G>A p.C1173Y EX22 2.8% missensePE356 LRP1B c.11258-1G>T . IVS73 20.0% splice-3PE378 ERBB2 c.2313_2324dupATACGTGATGGCp.Y772_A775dup EX20 6.0% cds-insPE278 F8 c.5038G>A p.E1680K EX14 8.8% missensePE448 RAD51B c.85-1G>A . IVS2 1.8% splice-3PE594 JAK2 c.1849G>T p.V617F EX14 0.5% missensePE540 TP53 c.916C>T p.R306* EX8 60.9% nonsensePE719 MET c.3028+3A>G . IVS14 33.1% intronPE643 IGF2 c.473C>T p.P158L EX4 14.9% missensePE689 MED12 c.4196C>G p.S1399C EX30 4.0% missensePE755 HIST1H3F c.292G>C p.E98Q EX1E 6.9% missensePE680 PALB2 c.568T>A p.S190T EX4 13.9% missensePE686 EGFR c.2573T>G p.L858R EX21 22.7% missensePE657 FOXA1 c.976C>A p.P326T EX2E 1.2% missense

PE714 RICTOR c.4876T>A p.S1626T EX36 2.1% missensePE891 CSF1R c.127G>A p.V43M EX3 3.3% missensePE934 PRKAR1A c.215G>A p.G72D EX3 1.4% missensePE869 FAT2 c.11862C>A p.N3954K EX20 5.2% missensePE592 STK11 c.533_553delAGCCGGGGAACCTGCTGCTCAp.K178_L184del EX4 4.7% cds-delPE284 DNMT3A c.1598A>G p.Y533C EX14 1.4% missensePE302 EGFR c.2235_2249delGGAATTAAGAGAAGCp.E746_A750del EX19 39.9% cds-delPE305 DNMT3A c.890G>A p.W297* EX8 0.7% nonsensePE310 EPHA5 c.1300C>G p.H434D EX5 7.2% missensePE322 NOTCH3 c.5357G>T p.G1786V EX29 57.1% missensePE424 MLL3 c.4999G>A p.E1667K EX33 1.4% missensePE534 NBN c.1030C>A p.Q344K EX9 3.2% missensePE523 ROBO3 c.1298T>C p.I433T EX8 25.3% missensePE421 RSPO2 c.594G>A p.M198I EX5 3.1% missensePE581 NF1 c.787A>T p.K263* EX8 30.4% nonsensePE625 EGFR c.2573T>G p.L858R EX21 9.7% missensePE687 PCK1 c.589G>A p.G197R EX4 4.4% missensePE650 EGFR c.2390G>C p.C797S EX20 30.1% missensePE812 LRP1B c.3259G>C p.G1087R EX21 6.3% missensePE798 TOP2A c.3466G>C p.D1156H EX27 1.2% missensePE877 ARID1A c.1753C>G p.Q585E EX3 1.6% missensePE591 IL7R c.394C>G p.P132A EX4 22.2% missensePE833 EGFR c.2369C>T p.T790M EX20 9.6% missensePE937 TP53 c.631A>G p.T211A EX6 4.1% missensePE220 CIITA c.1564C>T p.P522S EX11 7.7% missensePE325 TP53 c.659A>G p.Y220C EX6 45.0% missensePE336 PIK3CA c.1624G>A p.E542K EX10 20.7% missensePE208 DNMT3A c.1135C>T p.R379C EX10 1.2% missensePE363 IRS2 c.1025C>G p.S342W EX1 38.1% missensePE322 NOTCH2 c.2606G>T p.R869L EX17 6.4% missensePE429 CDKN2A c.28_31delGAGCinsCTp.E10Lfs*4 EX1 31.7% frameshiftPE461 NTRK1 c.1114G>A p.A372T EX8 3.2% missensePE417 ATR c.3890T>A p.V1297D EX21 22.5% missensePE537 ATM c.9022C>T p.R3008C EX63E 0.6% missensePE555 TP53 c.722C>T p.S241F EX7 3.3% missensePE531 PIK3CA c.112C>T p.R38C EX2 1.4% missensePE594 RAD51C c.635G>A p.R212H EX4 29.0% missensePE561 EP300 c.4283T>A p.L1428* EX26 31.8% nonsensePE629 AXIN2 c.953G>A p.S318N EX3 53.9% missensePE601 STAG2 c.2692G>A p.D898N EX27 1.5% missensePE761 AR c.2261C>A p.S754Y EX5 3.9% missensePE749 CYP2C8 c.782G>C p.R261P EX5 9.8% missensePE840 APC c.7600G>A p.E2534K EX16E 1.9% missensePE774 APC c.2178A[2>1] p.R727Gfs*34 EX16E 5.1% frameshiftPE774 EGFR c.2236_2250delGAATTAAGAGAAGCAp.E746_A750del EX19 13.8% cds-delPE853 KDM6A c.188A>G p.H63R EX2 4.6% missensePE828 TP53 c.817C>T p.R273C EX8 66.3% missensePE342 PPP2R1A c.742C>G p.L248V EX6 4.4% missensePE356 EPHA2 c.1918G>A p.E640K EX11 25.0% missensePE346 APC c.637C>T p.R213* EX6 5.3% nonsensePE404 BRAF c.1406G>C p.G469A EX11 9.3% missensePE468 CDK12 c.4366G[2>1] p.G1456Afs*43 EX14E 29.4% frameshiftPE406 DNMT3A c.703G>T p.E235* EX7 0.9% nonsensePE556 POT1 c.1480A>C p.I494L EX15 1.3% missense

PE594 TET2 c.2332C>T p.Q778* EX3 0.7% nonsensePE411 ATRX c.4375G>T p.E1459* EX15 28.7% nonsensePE673 NFE2L2 c.77A>C p.Q26P EX2 39.3% missensePE701 MED12 c.6223C>T p.Q2075* EX42 59.3% nonsensePE647 IRS2 c.4011A>T p.K1337N EX1 31.3% missensePE682 SLX4 c.3310C>T p.R1104W EX12 11.0% missensePE657 EPHB1 c.1451C>T p.A484V EX7 9.0% missensePE867 EPHA4 c.423G>C p.Q141H EX3 12.6% missensePE886 KDM5C c.2663_2678delGTGAGGCCCTGGCCTCp.R888Hfs*42 EX19 7.5% frameshiftPE735 NF1 c.7697C>T p.S2566L EX52 5.6% missensePE891 MLH1 c.1559-1G>T . IVS13 3.6% splice-3PE934 GAB2 c.1979A>T p.E660V EX10E 5.7% missensePE592 TP53 c.736A>T p.M246L EX7 5.3% missensePE918 PTEN c.350_360delATCATGTTGCAp.N117Sfs*5 EX5 16.2% frameshiftPE250 TP53 c.993+1G>T . IVS9 16.1% splice-5PE270 HGF c.865G>C p.A289P EX7 5.2% missensePE223 LRP1B c.7534_7535delGCinsAAp.A2512N EX46 1.0% missensePE276 RBM10 c.1427A>G p.Q476R EX12 59.1% missensePE207 ROBO3 c.2327C>T p.A776V EX15 26.0% missensePE356 SMARCA4 c.3030_3040delCATGCAGGCCAp.H1010Qfs*7 EX21 60.8% frameshiftPE394 CNTN5 c.133C>G p.L45V EX4 5.7% missensePE446 TP53 c.722C>T p.S241F EX7 0.5% missensePE542 FLT4 c.3473C>T p.A1158V EX26 2.0% missensePE539 ERG c.293A>G p.D98G EX3 1.3% missensePE507 CDKN2C c.250G>C p.D84H EX2E 1.7% missensePE593 SETD2 c.590C>T p.A197V EX3 15.7% missensePE514 RBM10 c.1883_1888+3delAGTACCGTG. EX15-IVS159.6% spanPE654 MLL3 c.1138C>T p.R380C EX8 5.7% missensePE673 TP53 c.615T[3>4] p.L206Ffs*3 EX6 47.6% frameshiftPE713 EGFR c.2369C>T p.T790M EX20 25.3% missensePE767 ATM c.6151C>G p.L2051V EX42 3.6% missensePE788 LRP1B c.3665T>C p.L1222P EX23 1.9% missensePE565 BRCA2 c.9086C>T p.A3029V EX23 0.9% missensePE794 AKT1 c.394G>A p.E132K EX5 9.9% missensePE850 PCK1 c.1576C>T p.L526F EX10E 3.3% missensePE891 BRCA1 c.2718G>T p.K906N EX10 3.9% missensePE869 IKZF1 c.691G>T p.G231C EX6 4.4% missensePE219 TP53 c.701A>G p.Y234C EX7 5.9% missensePE215 NF1 c.4378G>C p.D1460H EX33 8.5% missensePE461 PALB2 c.1697G>A p.R566H EX5 3.2% missensePE532 ASXL1 c.1961C[2>3] p.T655Hfs*3 EX13E 0.7% frameshiftPE417 FGF6 c.334G>T p.E112* EX1 82.4% nonsensePE508 BRAF c.1799T>A p.V600E EX15 1.6% missensePE572 BMPR1A c.1474-2A>T . IVS12 34.9% splice-3PE702 CD22 c.890G>A p.R297H EX5 31.2% missensePE599 MLL3 c.9203dupA p.Q3069Afs*18 EX38 48.7% frameshiftPE746 MYO3A c.3109A>G p.K1037E EX27 31.4% missensePE622 EGFR c.1412A>G p.Y471C EX12 0.7% missensePE679 BCOR c.4452G>C p.E1484D EX11 1.8% missensePE814 HNF1A c.1366C>G p.L456V EX7 0.5% missensePE774 ZFHX3 c.8287G>A p.D2763N EX9 5.2% missensePE858 PTCH1 c.4013G>A p.R1338H EX23 1.0% missensePE798 EGFR c.2155G>T p.G719C EX18 1.6% missensePE884 TSC1 c.3172C>G p.P1058A EX23E 37.7% missense

PE905 BRAF c.1799T>A p.V600E EX15 8.1% missensePE801 FAS c.639A[2>3] p.T214Nfs*5 EX7 1.2% frameshiftPE890 FOXP1 c.1871G>A p.R624K EX16 1.2% missensePE913 DNMT3A c.439G>C p.A147P EX4 23.9% missensePE223 BRAF c.1799T>A p.V600E EX15 1.1% missensePE207 ARID1B c.3772G>A p.A1258T EX15 2.2% missensePE338 BRCA2 c.155A>G p.H52R EX3 29.9% missensePE339 NF1 c.3867C>G p.F1289L EX28 1.0% missensePE429 APC c.7477C>G p.L2493V EX16E 16.0% missensePE555 ELF3 c.292G>C p.D98H EX3 4.1% missensePE525 PCK1 c.809T>G p.I270R EX6 2.8% missensePE520 MLLT3 c.471CAG[10>9] p.S149[42>41] EX5 11.0% cds-delPE516 TOP2A c.4093_4105delAGTAACAAAGAACp.S1365*fs*1 EX32 5.2% frameshiftPE572 MSH4 c.2365C>A p.L789M EX18 57.4% missensePE437 MAP2K4 c.1069A>G p.K357E EX10 20.4% missensePE777 KDM5C c.2768G>A p.R923Q EX19 33.1% missensePE749 FLG c.8556G>T p.R2852S EX3E 4.1% missensePE840 HCLS1 c.731C>A p.S244Y EX10 7.1% missensePE863 ASXL1 c.2941G>C p.E981Q EX13E 22.9% missensePE855 ACIN1 c.979A>T p.R327* EX6 3.4% nonsensePE616 EGFR c.2369C>T p.T790M EX20 1.3% missensePE875 NCOR1 c.3358G>T p.E1120* EX25 0.6% nonsensePE869 RAD51D c.552G>T p.E184D EX6 2.2% missensePE828 BRAF c.1593G>T p.W531C EX13 1.0% missensePE922 EGFR c.2573T>G p.L858R EX21 80.1% missensePE252 TRAF5 c.798_805delCTTACACAp.D266Efs*9 EX9 3.8% frameshiftPE270 SDHB c.784G>C p.A262P EX8 17.9% missensePE264 BRD2 c.940C>A p.P314T EX6 49.9% missensePE351 MYH9 c.1588G>A p.E530K EX14 8.8% missensePE344 FAS c.125C>A p.T42N EX2 13.5% missensePE444 MLL3 c.10624A>G p.T3542A EX43 19.5% missensePE418 FGFR2 c.870G>T p.W290C EX7 4.2% missensePE487 ROBO2 c.172G>A p.V58I EX3 1.4% missensePE503 PIK3CG c.928C>A p.P310T EX2 3.3% missensePE517 CSF1R c.316C>T p.R106W EX4 2.7% missensePE620 EGFR c.2239_2248delinsCp.L747_A750delinsPEX19 0.6% cds-indelPE654 LRP1B c.2976C>A p.D992E EX20 6.3% missensePE689 ETV6 c.1081G>T p.E361* EX6 1.1% nonsensePE757 NF1 c.2407C>T p.Q803* EX20 56.2% nonsensePE787 CTNNB1 c.110C>T p.S37F EX3 1.8% missensePE840 ARID1B c.5200G>C p.D1734H EX20E 0.9% missensePE759 APC c.2178A[2>1] p.R727Gfs*34 EX16E 2.0% frameshiftPE724 EGFR c.2369C>T p.T790M EX20 5.4% missensePE753 TP53 c.714T>A p.C238* EX7 34.5% nonsensePE592 LRP1B c.10876A>G p.M3626V EX71 3.8% missensePE925 TP53 c.830G>T p.C277F EX8 73.2% missensePE223 PIK3CB c.2570C>G p.S857C EX18 1.2% missensePE222 POLD1 c.2549G>T p.R850L EX20 2.5% missensePE251 SERPINB3 c.1064C>A p.S355* EX8 0.7% nonsensePE350 EGFR c.2573T>G p.L858R EX21 13.6% missensePE245 TP53 c.867C[2>1] p.R290Afs*55 EX8 24.4% frameshiftPE352 EGFR c.2239_2240delTTinsCCp.L747P EX19 48.6% missensePE362 NOTCH1 c.2972C>T p.S991F EX19 1.6% missensePE356 ARID2 c.1116G>A p.M372I EX9 17.9% missense

PE378 CHEK2 c.433C>T p.R145W EX3 1.4% missensePE460 RB1 c.361C>T p.Q121* EX3 54.1% nonsensePE500 EPHA5 c.2189A>T p.Q730L EX12 26.3% missensePE539 IRS2 c.197C>T p.S66L EX1 1.4% missensePE593 MLH1 c.2101C>A p.Q701K EX18 24.1% missensePE612 RB1 c.644C>A p.S215* EX7 67.4% nonsensePE557 TP53 c.794T>C p.L265P EX8 6.9% missensePE728 PIK3CA c.1255_1261delCACTGTCinsAp.H419_P421delinsTEX8 1.9% cds-indelPE705 MAF c.101A>G p.K34R EX1E 0.5% missensePE495 RYR1 c.2500C>T p.R834W EX20 4.7% missensePE794 FAT1 c.3749A>T p.Y1250F EX5 23.9% missensePE824 TET2 c.5618T>C p.I1873T EX11E 1.0% missensePE840 ERCC3 c.2251T>G p.S751A EX15E 17.7% missensePE724 PALB2 c.2128A>G p.T710A EX5 3.8% missensePE878 CYP2C8 c.241G>A p.E81K EX2 4.1% missensePE915 KEAP1 c.1253_1254dupTGp.G419Wfs*40 EX3 37.5% frameshiftPE891 C11orf30 c.692T>C p.I231T EX7 3.6% missensePE895 MET c.3028+2T>C . IVS14 95.7% splice-5PE935 PTPRD c.104G>T p.G35V EX13 11.2% missensePE269 SDHB c.784G>C p.A262P EX8 1.1% missensePE274 SETD2 c.4310C>T p.S1437L EX3 6.8% missensePE252 MED12 c.514G>A p.E172K EX4 36.0% missensePE349 TP53 c.517G>T p.V173L EX5 15.2% missensePE331 EGFR c.2573T>G p.L858R EX21 7.9% missensePE368 MAP2K1 c.157T>G p.F53V EX2 3.4% missensePE340 ARAP3 c.1537G>A p.D513N EX10 1.5% missensePE418 LRP1B c.1286C>G p.T429S EX9 3.0% missensePE457 RNF43 c.1244G>C p.G415A EX9 1.1% missensePE358 FLG c.8569C>T p.R2857C EX3E 2.3% missensePE578 BRD4 c.1195G>T p.D399Y EX6 19.5% missensePE510 DAXX c.1736T>G p.F579C EX6 15.0% missensePE551 TMPRSS2 c.311C>T p.T104M EX3 1.2% missensePE599 ATR c.5537G>A p.R1846Q EX32 3.6% missensePE496 EGFR c.2369C>T p.T790M EX20 10.9% missensePE733 ELF3 c.367G>T p.A123S EX3 12.1% missensePE669 PTEN c.963A[6>7] p.N323Kfs*2 EX8 7.5% frameshiftPE689 JAK2 c.2203G>C p.D735H EX17 1.5% missensePE793 TP53 c.992A>T p.Q331L EX9 9.6% missensePE753 TGFBR1 c.125C>G p.T42R EX2 1.3% missensePE892 EPHB1 c.2608C>T p.R870W EX14 8.8% missensePE893 EGFR c.2306_2307insTGCCAGCGTp.A767_V769dup EX20 48.9% cds-insPE870 TP53 c.1016delA p.E339Gfs*6 EX10 7.5% frameshiftPE252 BCOR c.4364G>C p.R1455T EX10 7.6% missensePE264 NCOR1 c.3332C>T p.S1111F EX25 2.0% missensePE335 FAT2 c.11394C>G p.F3798L EX19 1.6% missensePE444 MLL2 c.10744C>T p.R3582W EX39 1.0% missensePE636 SMAD4 c.1521A>T p.K507N EX12E 36.0% missensePE647 IDH1 c.394C>T p.R132C EX4 17.9% missensePE644 POLD1 c.2728C>T p.R910W EX22 12.5% missensePE689 PLCG2 c.1736G>T p.R579L EX18 2.1% missensePE682 EGFR c.2369C>T p.T790M EX20 15.9% missensePE722 EGFR c.2573T>G p.L858R EX21 46.6% missensePE866 EGFR c.2573T>G p.L858R EX21 13.9% missensePE886 ERBB2 c.2313_2324dupATACGTGATGGCp.Y772_A775dup EX20 7.9% cds-ins

PE863 EGFR c.2236_2250delGAATTAAGAGAAGCAp.E746_A750del EX19 40.7% cds-delPE896 SF3B1 c.2221A>C p.K741Q EX15 0.9% missensePE934 FGF10 c.577C>T p.R193* EX3E 5.1% nonsensePE592 KRAS c.34G>T p.G12C EX2 7.8% missensePE907 TP53 c.722C>T p.S241F EX7 82.4% missensePE932 POLD1 c.1322C>T p.T441M EX11 0.6% missensePE816 U2AF1 c.173C>G p.S58C EX3 2.5% missensePE207 PIK3CG c.368G>A p.R123H EX2 2.2% missensePE353 RBM10 c.1258-1G>A . IVS10 20.9% splice-3PE338 MLL3 c.9311T[2>1] p.G3106Vfs*2 EX39 21.8% frameshiftPE339 SMARCA4 c.4197G>T p.E1399D EX30 1.2% missensePE332 EGFR c.2573T>G p.L858R EX21 24.2% missensePE483 BMPR1A c.779T>C p.V260A EX9 1.3% missensePE417 SPTA1 c.6621G>T p.Q2207H EX48 21.1% missensePE555 ELF3 c.999G>A p.M333I EX8 5.0% missensePE525 ATRX c.4916A>G p.E1639G EX18 16.8% missensePE594 CYP1A1 c.1364G>A p.R455Q EX7E 1.7% missensePE580 BRAF c.1799T>A p.V600E EX15 7.5% missensePE783 RNASEL c.1003G>C p.D335H EX2 12.4% missensePE749 NFE2L2 c.235G>A p.E79K EX2 4.2% missensePE850 MAP3K1 c.1105G>A p.E369K EX5 17.6% missensePE863 ASXL1 c.3111G>C p.W1037C EX13E 25.1% missensePE857 PPP6C c.547C>A p.Q183K EX6 22.5% missensePE616 GRM3 c.2248T>G p.L750V EX4 1.2% missensePE875 CDK12 c.632A>T p.K211I EX1 9.3% missensePE631 MAP2K2 c.787G>A p.G263R EX7 1.4% missensePE828 MLL3 c.7443-2_7443-1insAA. IVS37 4.2% splice-3PE322 SLX4 c.1888G>A p.G630S EX8 1.5% missensePE369 SETBP1 c.1602C>A p.C534* EX4 6.9% nonsensePE424 PMS2 c.-87-u95G>A . . 1.2% promoterPE361 PPM1D c.1651A>T p.R551* EX6E 1.0% nonsensePE472 FOXA2 c.457G>T p.D153Y EX2E 2.0% missensePE576 IGF1R c.880G>A p.E294K EX3 3.4% missensePE474 CTNNB1 c.110C>G p.S37C EX3 0.6% missensePE638 CDH1 c.1727C>A p.T576N EX12 1.2% missensePE690 MLL2 c.12855C[3>2] p.P4286Hfs*98 EX39 23.2% frameshiftPE644 MLL3 c.1289G>A p.W430* EX9 12.4% nonsensePE687 NTRK3 c.295A>G p.M99V EX4 2.0% missensePE748 MLL3 c.13312G>A p.E4438K EX52 5.2% missensePE926 TBX3 c.1831G>A p.A611T EX8E 9.6% missensePE937 EGFR c.2573T>G p.L858R EX21 11.9% missensePE218 GATA3 c.340C>T p.L114F EX3 1.3% missensePE302 BCAS1 c.1306G>A p.E436K EX9 29.8% missensePE319 FAT2 c.10015G>A p.V3339I EX14 17.4% missensePE310 SMAD4 c.1082G>A p.R361H EX9 9.1% missensePE391 EP300 c.4854_4855insCCTCTGp.P1617_L1618dupEX30 20.1% cds-insPE364 ARID1A c.5686G>T p.G1896W EX20E 13.6% missensePE523 MECOM c.1336C>A p.P446T EX8 21.0% missensePES121 KRAS c.35G>A p.G12D EX2 62.7% missensePE547 FCGR2B c.169C>T p.Q57* EX3 3.2% nonsensePE421 CDH1 c.727G>A p.E243K EX6 13.2% missensePE474 RELN c.1047G>T p.L349F EX10 0.6% missensePE588 BRD2 c.1085C>A p.S362Y EX6 1.3% missensePE737 JAK3 c.3098A>T p.E1033V EX23 7.0% missense

PE690 TP53 c.712T>C p.C238R EX7 30.0% missensePE726 F8 c.4868G>A p.C1623Y EX14 3.9% missensePE689 LRP1B c.3867C>G p.N1289K EX24 2.1% missensePE650 SPOP c.208C>T p.R70* EX4 1.1% nonsensePE667 EGFR c.2235_2249delGGAATTAAGAGAAGCp.E746_A750del EX19 2.1% cds-delPE678 TET2 c.949C>T p.Q317* EX3 3.7% nonsensePE812 WT1 c.1376A>C p.K459T EX9 5.2% missensePE911 NTRK3 c.385T>G p.L129V EX5 1.8% missensePE826 EGFR c.2237_2252delAATTAAGAGAAGCAACinsTp.E746_T751delinsVEX19 70.5% cds-indelPE591 KEAP1 c.380_395delGTATCCACCCCAAGGTp.G127Afs*25 EX2 26.9% frameshiftPE821 XPO1 c.793C>G p.L265V EX10 3.4% missensePE833 TP53 c.856G>T p.E286* EX8 33.0% nonsensePE219 BRCA2 c.1857G>T p.Q619H EX10 4.4% missensePE223 TP53 c.818G>A p.R273H EX8 0.2% missensePE336 FGFR2 c.1223delA p.D408Afs*11 EX9 4.8% frameshiftPE215 PIK3CA c.346G>C p.E116Q EX2 5.7% missensePE322 NFE2L2 c.239C>T p.T80I EX2 38.7% missensePE403 ROBO3 c.2692G>C p.G898R EX17 1.0% missensePE436 TET2 c.3785G>A p.R1262Q EX6 7.8% missensePE472 LRP1B c.11024G>T p.R3675I EX72 5.2% missensePE461 RET c.1423C>T p.R475W EX7 1.4% missensePE417 PIK3CG c.2896G[3>2] p.H967Tfs*21 EX10 12.4% frameshiftPE531 PTEN c.274G>C p.D92H EX5 18.3% missensePE505 ATRX c.3458C>T p.S1153L EX9 4.2% missensePE586 RARA c.574C>T p.R192C EX5 25.2% missensePE702 EGFR c.2237_2251delAATTAAGAGAAGCAAp.E746_T751delinsAEX19 14.5% cds-indelPE746 MLL3 c.5009-1G>C . IVS33 18.9% splice-3PE481 EGFR c.2235_2249delGGAATTAAGAGAAGCp.E746_A750del EX19 27.0% cds-delPE671 EPHA3 c.659C>T p.S220F EX3 3.5% missensePE622 APC c.3172G>A p.D1058N EX16E 0.6% missensePE814 EGFR c.2369C>T p.T790M EX20 1.1% missensePE697 FAT1 c.1490A>T p.Y497F EX2 16.9% missensePE749 TP53 c.461G>T p.G154V EX5 10.3% missensePE774 CYP2D6 c.265G>A p.E89K EX2 2.9% missensePE858 INPP4B c.2446A[7>8] p.R818Kfs*26 EX24 13.4% frameshiftPE252 FLT4 c.3967G>A p.E1323K EX30 3.8% missensePE270 TBX3 c.1284G[2>1] p.D429Tfs*203 EX7 13.3% frameshiftPE334 GRIN2A c.2884G>C p.E962Q EX13 14.9% missensePE264 LRP1B c.877C>A p.L293I EX7 38.2% missensePE335 EGFR c.2125G>A p.E709K EX18 9.4% missensePE378 RUNX2 c.706G>A p.D236N EX6 4.6% missensePE364 NOTCH3 c.3004G>T p.D1002Y EX19 1.2% missensePE477 EGFR c.122T>G p.L41W EX2 22.6% missensePE418 TP53 c.743G>A p.R248Q EX7 6.4% missensePE445 WASF3 c.1188delAinsCC p.P401Tfs*26 EX9 5.3% frameshiftPE411 GALNT12 c.889C>G p.R297G EX4 28.0% missensePE586 LRP1B c.1991G>T p.W664L EX13 31.2% missensePE654 EPHA3 c.607C>A p.P203T EX3 1.0% missensePE628 TP53 c.470T>G p.V157G EX5 18.8% missensePE599 PSMB5 c.602G>T p.G201V EX3E 4.3% missensePE660 ARAF c.1458C>G p.Y486* EX14 6.1% nonsensePE682 TRIM58 c.613G>C p.E205Q EX3 4.3% missensePE705 FCGR2A c.364T>C p.W122R EX4 2.2% missensePE680 SF3B1 c.1359G>A p.M453I EX10 7.7% missense

PE765 IKBKE c.1499G>A p.R500Q EX14 2.2% missensePE840 BRCA2 c.294A>C p.L98F EX3 1.6% missensePE759 MLL2 c.8944G>C p.E2982Q EX34 1.3% missensePE888 FAT2 c.9754G>A p.G3252S EX13 2.5% missensePE886 MET c.2389G>A p.E797K EX11 10.8% missensePE592 MLL3 c.8302G>C p.D2768H EX38 2.6% missensePE933 EGFR c.865_866delGCinsCTp.A289L EX7 5.8% missensePE297 MAGI2 c.2729G>A p.G910D EX16 9.1% missensePE309 FLCN c.781C>T p.L261F EX8 33.0% missensePE207 SRC c.1062G[5>4] p.E356Rfs*28 EX11 19.6% frameshiftPE356 EP300 c.5189C>T p.S1730F EX31 14.5% missensePE503 MEF2C c.410C>T p.P137L EX6 17.1% missensePE546 MSR1 c.371G>T p.R124I EX4 1.5% missensePE554 BRAF c.1799T>A p.V600E EX15 0.4% missensePE214 PIK3CA c.1624G>A p.E542K EX10 6.5% missensePE593 MSH3 c.3181A>G p.R1061G EX23 6.1% missensePE664 AKT3 c.329A>G p.Q110R EX4 4.5% missensePE654 NCOR1 c.1532A>T p.E511V EX15 38.1% missensePE612 MED12 c.1850C>G p.T617S EX13 70.0% missensePE535 TGFBR1 c.343+1G>T . IVS2 4.7% splice-5PE385 MS4A1 c.440T>A p.L147* EX6 12.4% nonsensePE694 DPYD c.2255C>A p.P752Q EX18 32.0% missensePE649 FANCF c.262C>G p.H88D EX1E 8.5% missensePE788 EGFR c.2573T>G p.L858R EX21 6.1% missensePE800 TNFAIP3 c.1685G>T p.R562L EX7 15.1% missensePE876 EGFR c.2573T>G p.L858R EX21 6.3% missensePE854 LRP1B c.433G>A p.E145K EX4 1.1% missensePE250 POLE c.553G>C p.D185H EX6 11.0% missensePE254 EGFR c.2156G>C p.G719A EX18 1.7% missensePE276 TAF1L c.4208C>T p.T1403M EX1 24.8% missensePE207 SETD2 c.7573A>G p.K2525E EX21 25.4% missensePE431 EGFR c.2235_2249delGGAATTAAGAGAAGCp.E746_A750del EX19 0.6% cds-delPE394 KRAS c.35G>C p.G12A EX2 64.0% missensePE490 ZFHX3 c.10295C>T p.S3432F EX10E 0.5% missensePE500 CDK12 c.46G>A p.G16R EX1 9.1% missensePE542 TP53 c.493C>T p.Q165* EX5 3.6% nonsensePE487 EGFR c.2573T>G p.L858R EX21 71.7% missensePE539 AR c.2265C>A p.F755L EX5 17.8% missensePE593 CDH23 c.1282G>A p.D428N EX13 13.0% missensePE654 RAD21 c.1206A[5>3] p.R404Efs*8 EX10 18.0% frameshiftPE713 PIK3CG c.1266C>G p.D422E EX2 20.6% missensePE728 MLL3 c.4873G>T p.E1625* EX33 0.5% nonsensePE668 ABL1 c.2455C>T p.R819W EX11E 27.3% missensePE767 TP53 c.1025G[1>4] p.R342_E343insGEX10 1.8% cds-insPE717 TP53 c.417G>C p.K139N EX5 4.9% missensePE819 POLE c.2187A>T p.K729N EX20 1.3% missensePE791 PTPRB c.256C>A p.R86S EX2 0.7% missensePE876 INPP4B c.1932G>C p.Q644H EX20 0.8% missensePES130 CDKN2A c.150+1_150+2insG. IVS1 0.5% splice-5PE308 SERPINB3 c.748G>C p.E250Q EX7 0.8% missensePE216 ABCC11 c.2401G>T p.G801* EX18 3.2% nonsensePE303 MLH3 c.3428C>G p.S1143* EX4 4.0% nonsensePE207 FAT1 c.839A>G p.D280G EX2 12.5% missensePE390 MTOR c.3114G>C p.M1038I EX20 1.0% missense

PE413 PTPN11 c.155C>T p.T52I EX3 24.9% missensePE485 PMS1 c.440G>T p.R147I EX5 7.1% missensePE442 BRSK1 c.1258_1259delGCinsTAp.A420Y EX12 2.1% missensePE513 MLL3 c.8648A>T p.N2883I EX38 0.5% missensePE594 EPAS1 c.1960G>A p.V654I EX12 1.6% missensePE629 EGFR c.2303G>T p.S768I EX20 77.5% missensePE642 EGFR c.2573T>G p.L858R EX21 12.1% missensePE730 NTRK2 c.208G>A p.E70K EX4 2.1% missensePE716 ARID1A c.4465_4489delGCTGAGGTTGCTCAGCAAGGCACCAp.A1489Cfs*8 EX18 10.3% frameshiftPE808 NPM1 c.517G>A p.D173N EX6 4.0% missensePE807 EPHA7 c.628G>C p.E210Q EX3 26.5% missensePE922 APC c.7546G>A p.E2516K EX16E 18.9% missensePE252 MLL4 c.3185C>A p.P1062Q EX8 3.6% missensePE264 TP53 c.818G>T p.R273L EX8 2.3% missensePE335 DNMT3A c.1474+1G>A . IVS12 1.7% splice-5PE424 RBM10 c.1912C>T p.Q638* EX16 2.0% nonsensePE411 IDH2 c.802G>C p.E268Q EX6 7.9% missensePE599 CHEK2 c.1435G>A p.E479K EX13 3.3% missensePE689 CTCF c.313G>C p.E105Q EX3 1.7% missensePE722 EGFR c.2500G>T p.V834L EX21 45.0% missensePE686 EPHA2 c.1072G>A p.E358K EX5 11.0% missensePE809 EPHB6 c.1087G>C p.E363Q EX8 2.0% missensePE866 IL6 c.66G>T p.L22F EX2 1.1% missensePE886 TP53 c.273G>A p.W91* EX4 13.6% nonsensePE863 ROS1 c.4540A>T p.I1514L EX28 23.9% missensePE934 PIK3CA c.1349_1366delATGGATTAGAAGATTTGCp.H450_L455del EX8 4.6% cds-delPE592 FGF23 c.597G>A p.M199I EX3E 3.9% missensePE918 HIST1H3B c.398G>A p.G133E EX1E 2.5% missensePE250 EGFR c.3382C>A p.P1128T EX28 13.3% missensePE254 EGFR c.2582T>G p.L861R EX21 1.8% missensePE276 RBM10 c.1430G[3>2] p.A478Pfs*72 EX12 55.3% frameshiftPE207 KEAP1 c.326C>T p.A109V EX2 25.8% missensePE356 PIK3R2 c.1549G>C p.E517Q EX12 14.4% missensePE401 EGFR c.2573_2574delTGinsGTp.L858R EX21 4.7% missensePE431 MAP3K1 c.623G>A p.R208Q EX2 0.7% missensePE394 MLL c.3242G>A p.R1081Q EX4 10.6% missensePE500 TP53 c.796G>A p.G266R EX8 36.7% missensePE539 CRLF2 c.382T>C p.S128P EX4 1.7% missensePE507 RICTOR c.1262_1265delTTAGp.V421Efs*6 EX15 34.1% frameshiftPE584 EGFR c.2237_2255delAATTAAGAGAAGCAACATCinsTp.E746_S752delinsVEX19 7.5% cds-indelPE593 ARAP3 c.1412G>A p.R471Q EX10 13.2% missensePE612 ERBB2 c.2326_2327insTCGTGATGGCTGp.A775_G776insVVMAEX20 49.1% cds-insPE767 BRCA2 c.7743G>C p.L2581F EX16 1.6% missensePE717 ATM c.8663T>C p.I2888T EX59 0.6% missensePE788 EPHA3 c.2917G>T p.E973* EX17E 10.1% nonsensePE794 MAP2K1 c.105G>C p.K35N EX2 4.6% missensePE810 ERRFI1 c.158C>T p.A53V EX3 17.3% missensePE876 FAM175A c.670G>A p.E224K EX7 2.4% missensePE891 SOX9 c.484C>T p.R162C EX2 3.0% missensePE303 ALK c.4034G>C p.G1345A EX27 8.1% missensePE207 BRIP1 c.1276G>T p.V426F EX9 18.2% missensePE204 EGFR c.2240_2257delTAAGAGAAGCAACATCTCp.L747_P753delinsSEX19 40.7% cds-indelPE416 EGFR c.2573T>G p.L858R EX21 7.0% missensePE453 TP53 c.586C>T p.R196* EX6 7.2% nonsense

PE503 COL5A3 c.770G>A p.R257Q EX6 13.2% missensePES121 IRS2 c.3515A>T p.H1172L EX1 0.4% missensePE214 EP300 c.4307G>C p.W1436S EX27 8.2% missensePE561 NFE2L2 c.245A>G p.E82G EX2 31.5% missensePE637 STAG2 c.288+1G>A . IVS5 1.6% splice-5PE354 PIK3R1 c.320A>G p.D107G EX2 10.9% missensePE728 MAPK1 c.964G>A p.E322K EX7 3.8% missensePE760 EGFR c.2239_2248delinsCp.L747_A750delinsPEX19 35.2% cds-indelPE745 PLXNB2 c.4324A>T p.K1442* EX27 2.8% nonsensePE679 ERBB4 c.3927A>T p.*1309Yfs*? EX28E 10.8% stop-lossPE840 EGFR c.2239_2240delTTinsCCp.L747P EX19 47.2% missensePE792 RBM10 c.881G>A p.R294H EX8 3.1% missensePE773 TP53 c.536A>G p.H179R EX5 2.3% missensePE692 EGFR c.2239_2248delinsCp.L747_A750delinsPEX19 0.4% cds-indelPES130 XPO1 c.2977C>G p.Q993E EX24 1.5% missensePE212 RNF43 c.1882G>A p.D628N EX9 0.9% missensePE217 BRIP1 c.496A>G p.T166A EX5 3.3% missensePE260 TMPRSS2 c.546G>C p.E182D EX5 0.7% missensePE263 PBRM1 c.2641A[5>6] p.N882Kfs*19 EX18 36.5% frameshiftPE207 EMID2 c.268G>A p.A90T EX2 7.1% missensePE279 TP53 c.811G>T p.E271* EX8 2.4% nonsensePE606 CDKN2B c.326G>A p.R109H EX2 1.9% missensePE339 SYK c.1540G>C p.G514R EX11 15.7% missensePE329 TP53 c.532C>G p.H178D EX5 21.1% missensePE320 TSC2 c.3562C>T p.P1188S EX30 1.4% missensePE434 CREBBP c.6522G>A p.M2174I EX31E 5.8% missensePE450 STAG2 c.406T>C p.F136L EX7 2.9% missensePE454 EGFR c.2235_2249delGGAATTAAGAGAAGCp.E746_A750del EX19 55.9% cds-delPE497 TP53 c.672G>T p.E224D EX6 2.5% missensePE569 EGFR c.2573T>G p.L858R EX21 2.1% missensePE731 RBM10 c.2185A>T p.K729* EX18 20.4% nonsensePE764 MLL3 c.1271C>T p.S424L EX9 9.5% missensePE718 TBX3 c.1339C>G p.R447G EX7 1.7% missensePE437 EGFR c.2235_2249delGGAATTAAGAGAAGCp.E746_A750del EX19 51.5% cds-delPE696 CIC c.1540C>A p.P514T EX10 0.5% missensePE608 PTPRD c.4297C>A p.P1433T EX37 1.2% missensePE832 PSG2 c.781T>A p.C261S EX4 2.5% missensePE684 EGFR c.2237_2256delAATTAAGAGAAGCAACATCTinsTCp.E746_S752delinsVEX19 33.1% cds-indelPE849 EGFR c.2235_2249delGGAATTAAGAGAAGCp.E746_A750del EX19 9.3% cds-delPE772 MLL3 c.1207A>G p.M403V EX9 13.6% missensePE710 TP53 c.1010G>T p.R337L EX10 17.7% missensePE898 MET c.3480G>T p.M1160I EX17 1.0% missensePE875 BRAF c.1406G>C p.G469A EX11 0.7% missensePE912 TP53 c.539_542dupAGCGp.C182Afs*5 EX5 60.5% frameshiftPE894 CDKN1A c.25C>T p.R9C EX3 3.0% missensePE801 ERBB4 c.3043G>T p.D1015Y EX25 13.2% missensePE292 RUNX1 c.359C>T p.A120V EX5 1.5% missensePE378 SOX9 c.698G>C p.G233A EX3 2.1% missensePE346 GNAS c.601C>T p.R201C EX8 4.4% missensePE527 TTL c.601_603delATCinsGTTp.I201V EX4 17.2% missensePE539 DDR2 c.903_904delGGinsCTp.E301_V302delinsDLEX9 17.3% missensePE635 TP53 c.991C>T p.Q331* EX9 6.3% nonsensePE610 EGFR c.2240_2254delTAAGAGAAGCAACATp.L747_T751del EX19 5.9% cds-delPE708 FBXW7 c.1727C>T p.T576M EX11 2.1% missense

PE495 PIK3CA c.3140A>T p.H1047L EX21E 4.7% missensePE804 EGFR c.2235_2249delGGAATTAAGAGAAGCp.E746_A750del EX19 15.7% cds-delPE724 RB1 c.289G>T p.E97* EX3 15.1% nonsensePE867 BTK c.1844G>A p.R615H EX18 22.7% missensePE891 PIK3CG c.194G>A p.G65D EX2 3.3% missensePE275 EGFR c.2236_2250delGAATTAAGAGAAGCAp.E746_A750del EX19 37.1% cds-delPE215 RBM10 c.760G>T p.E254* EX6 27.6% nonsensePE374 TAL1 c.821_822insGGGGCGCp.A275Gfs*7 EX4E 3.8% frameshiftPE607 HIST1H3B c.220G>A p.E74K EX1E 1.1% missensePE461 ERBB2 c.2326delGinsTTATp.G776delinsLC EX20 72.4% cds-indelPE417 CREBBP c.6597G>T p.Q2199H EX31E 36.9% missensePE397 CUL3 c.1611-2_1616delAGGTTCTA. IVS11-EX123.1% spanPE528 EPHA2 c.445G>T p.E149* EX3 9.4% nonsensePE584 POLD1 c.470G>T p.G157V EX5 3.3% missensePE531 TP53 c.722C>T p.S241F EX7 17.0% missensePE639 TP53 c.1009C>T p.R337C EX10 9.8% missensePE508 HSP90AA1 c.1202A>T p.K401M EX6 2.0% missensePE704 EGFR c.2573T>G p.L858R EX21 32.1% missensePE746 KRAS c.34G>C p.G12R EX2 18.2% missensePE683 EGFR c.2236_2250delGAATTAAGAGAAGCAp.E746_A750del EX19 23.5% cds-delPE723 DOT1L c.544C>G p.H182D EX6 3.5% missensePE781 AURKB c.361C>T p.Q121* EX5 19.2% nonsensePE677 TGFBR2 c.1454G>T p.R485L EX6 8.7% missensePE858 KRAS c.34G>T p.G12C EX2 32.1% missensePE801 PDCD1 c.815G>A p.R272Q EX5E 16.7% missensePE913 RHOA c.167C>T p.A56V EX3 6.2% missensePE378 TP53 c.329G>T p.R110L EX4 16.4% missensePE278 EGFR c.2573T>G p.L858R EX21 10.7% missensePE406 PTEN c.95T>G p.I32S EX2 2.4% missensePE594 HIST1H2BCc.46A>G p.K16E EX1E 1.5% missensePE540 PDCD1LG2 c.713C[2>1] p.T239Qfs*2 EX5 62.3% frameshiftPE647 TP53 c.738G>A p.M246I EX7 18.2% missensePE484 EGFR c.2236_2252delGAATTAAGAGAAGCAACinsATp.E746_T751delinsIEX19 8.1% cds-indelPE682 TP53 c.991C>T p.Q331* EX9 19.8% nonsensePE641 EGFR c.2235_2249delGGAATTAAGAGAAGCp.E746_A750del EX19 3.0% cds-delPE680 ROBO3 c.3619G>C p.A1207P EX24 13.2% missensePE686 ARID1B c.986C>G p.A329G EX1 12.6% missensePE766 KDR c.2474G>A p.S825N EX17 1.9% missensePE657 MLL3 c.7085C>T p.S2362L EX36 1.2% missensePE714 HDAC4 c.3086_3104delGCTGCCTGCAGCGCACAACp.R1029Pfs*8 EX26 3.0% frameshiftPE735 RUNX1 c.362T>G p.L121R EX5 1.0% missensePE891 PBRM1 c.3478C>T p.R1160* EX23 4.6% nonsensePE918 DICER1 c.896C>T p.S299L EX8 25.0% missensePE341 PIK3CG c.1202C>T p.P401L EX2 1.5% missensePE433 HNF1A c.341G>A p.R114H EX2 2.6% missensePE424 POLE c.52G>A p.E18K EX1 1.5% missensePE364 ADAM29 c.2365C>T p.Q789* EX5E 1.0% nonsensePE323 HDAC4 c.2771C>T p.P924L EX23 11.2% missensePE443 EGFR c.2155G>T p.G719C EX18 0.3% missensePE457 MLL3 c.1159G>T p.E387* EX8 0.9% nonsensePE456 KRAS c.183A>C p.Q61H EX3 20.0% missensePE547 CRNKL1 c.354_356dupCCT p.L119dup EX2 6.3% cds-insPE474 CNTN5 c.131C>G p.S44C EX4 1.8% missensePE581 STK11 c.766G>T p.E256* EX6 91.9% nonsense

PE588 RARA c.574C>T p.R192C EX5 27.2% missensePE669 ERBB4 c.2966G>T p.G989V EX25 5.7% missensePE740 FAT2 c.3878T>A p.I1293N EX4 3.0% missensePE698 C11orf30 c.310C>G p.P104A EX5 25.3% missensePE793 LRP1B c.12603T>G p.Y4201* EX82 9.6% nonsensePE563 FBXW7 c.1313C>T p.S438F EX9 76.4% missensePE877 MLL c.2672A>C p.E891A EX3 4.6% missensePE885 EGFR c.2573T>G p.L858R EX21 34.0% missensePE735 FOXP1 c.940G>A p.E314K EX8 47.7% missensePE900 EGFR c.2369C>T p.T790M EX20 0.4% missensePE935 PDGFRB c.646G>A p.V216I EX5 17.4% missensePE219 RB1 c.1762delT p.S588Lfs*23 EX18 6.3% frameshiftPE336 NRAS c.35G>A p.G12D EX2 0.5% missensePE215 BLM c.1924G>A p.E642K EX8 11.4% missensePE322 SUCLG2 c.799G>C p.E267Q EX8 74.1% missensePE424 ARID1A c.232G>A p.E78K EX1 1.1% missensePE436 ARAP3 c.1537G>A p.D513N EX10 3.0% missensePE429 CREBBP c.3874C>T p.Q1292* EX22 14.7% nonsensePE461 RB1 c.2326-2A>T . IVS22 1.9% splice-3PE532 TP53 c.707A>G p.Y236C EX7 0.7% missensePE417 PREX2 c.2893dupA p.R965Kfs*4 EX24 22.9% frameshiftPE549 EGFR c.2235_2240delGGAATTinsAATTCCp.E746_L747delinsIPEX19 25.8% missensePE586 BRCA1 c.4408G>A p.E1470K EX13 24.4% missensePE587 MTOR c.6801C>G p.I2267M EX48 2.0% missensePE508 SETD2 c.6293+1delG . IVS14 1.4% splice-5PE514 EGFR c.2240_2257delTAAGAGAAGCAACATCTCp.L747_P753delinsSEX19 6.7% cds-indelPE572 BRD3 c.832C>T p.R278W EX6 5.0% missensePE702 FANCA c.1772G>C p.R591P EX19 10.6% missensePE566 EP300 c.2323A>G p.M775V EX13 9.0% missensePE622 PDGFRB c.1663C>T p.L555F EX11 1.5% missensePE222 TP53 c.1044G[2>1] p.E349Nfs*21 EX10 14.1% frameshiftPE723 FCGR3A c.364G>T p.D122Y EX5 3.0% missensePE749 SMARCB1 c.470G>T p.G157V EX4 8.9% missensePE840 EPHB6 c.1718G>C p.G573A EX11 1.9% missensePE874 TP53 c.493C>T p.Q165* EX5 2.2% nonsensePE911 ERRFI1 c.498CA[2>1] p.T167Rfs*3 EX4E 1.1% frameshiftPE922 CDH23 c.3574G>A p.V1192I EX30 13.1% missensePE890 JAK1 c.2042G>A p.R681Q EX15 1.5% missensePE424 PCDH18 c.2729G>A p.R910K EX3 1.6% missensePE438 NFE2L2 c.70_76delTGGAGGCinsGGAAAAAp.W24_Q26delinsGKKEX2 1.9% missensePE388 ABCA13 c.10327G>C p.D3443H EX30 4.4% missensePE448 TP53 c.710T>A p.M237K EX7 67.5% missensePE472 KRAS c.35G>T p.G12V EX2 16.1% missensePE468 RB1 c.2489+2T>A . IVS23 93.1% splice-5PES121 EGFR c.2573T>G p.L858R EX21 43.8% missensePES121 EGFR c.2303G>T p.S768I EX20 15.3% missensePES121 EGFR c.2303G>T p.S768I EX20 9.6% missensePE580 PCSK6 c.967A>G p.K323E EX7 7.7% missensePE638 PMS2 c.1634C>G p.S545C EX11 1.8% missensePE587 MLL c.7405G>C p.D2469H EX27 1.4% missensePE622 NOTCH3 c.4702C>T p.R1568W EX25 0.7% missensePE471 BAP1 c.345G>T p.M115I EX5 6.0% missensePE741 CREBBP c.7130C>G p.S2377* EX31E 2.5% nonsensePE820 SEMA6A c.142C>T p.R48W EX3 15.8% missense

PE703 EGFR c.2573T>G p.L858R EX21 48.6% missensePE884 BCL3 c.1324G>T p.G442C EX9E 37.0% missensePE909 EGFR c.2240_2257delTAAGAGAAGCAACATCTCp.L747_P753delinsSEX19 8.3% cds-indelPE938 TP53 c.451C>A p.P151T EX5 0.8% missensePE890 SERPINA7 c.457C>A p.L153I EX2 2.0% missensePE913 ROBO3 c.1366G>C p.G456R EX9 22.3% missensePE438 KIT c.711T>A p.D237E EX4 4.0% missensePE383 ARAP3 c.1537G>A p.D513N EX10 1.3% missensePES121 MAPK3 c.642G>C p.E214D EX4 2.5% missensePES121 KIF5B c.515T>G p.F172C EX7 0.5% missensePE520 APC c.4661A[6>7] p.T1556Nfs*3 EX16E 13.0% frameshiftPE638 EGFR c.2369C>T p.T790M EX20 5.7% missensePE737 MAML2 c.2852G>C p.R951T EX5E 1.4% missensePE683 TP53 c.481_482delGCinsTTp.A161F EX5 39.4% missensePE690 PTEN c.212G>T p.C71F EX4 30.0% missensePE666 TP53 c.574C>T p.Q192* EX6 2.0% nonsensePE880 ARID2 c.3926C>G p.S1309* EX15 45.7% nonsensePE703 ZNF804B c.349C>T p.Q117* EX3 19.6% nonsensePE750 EPHB6 c.797C>T p.A266V EX7 0.3% missensePE909 OR4C6 c.122T>G p.L41R EX1E 7.8% missensePE913 CCND2 c.518_519delAGinsCAp.K173T EX3 3.7% missensePE270 AKT3 c.1054G>A p.E352K EX10 12.7% missensePE223 BCOR c.4364G>C p.R1455T EX10 2.0% missensePE240 MAP2K1 c.313_318delCCCGCAp.P105_A106del EX3 2.1% cds-delPE350 ARID1B c.5407T>G p.S1803A EX20 1.4% missensePE207 STK11 c.837C[6>5] p.P281Rfs*6 EX6 36.5% frameshiftPE356 TP53 c.844C>T p.R282W EX8 90.7% missensePE394 EGFR c.2573T>G p.L858R EX21 15.8% missensePE366 BAP1 c.91G>A p.E31K EX3 2.7% missensePE447 EGFR c.2369C>T p.T790M EX20 7.8% missensePE428 ERRFI1 c.502G>C p.D168H EX4E 11.2% missensePE446 ABL2 c.955T[2>1] p.L319*fs*1 EX5 0.7% frameshiftPE490 ATM c.72+1G>T . IVS2 18.7% splice-5PE500 MLL3 c.2441C>G p.T814R EX14 6.0% missensePE539 TP53 c.796G>A p.G266R EX8 31.8% missensePE576 EGFR c.2152C>G p.L718V EX18 1.5% missensePE593 RAD51C c.1051G>A p.V351I EX9E 17.5% missensePE612 EME2 c.338G>A p.R113H EX2 0.5% missensePE644 TSC1 c.1063A>C p.M355L EX11 0.5% missensePE673 FGFR1 c.1028C>T p.A343V EX8 2.4% missensePE713 MYCL1 c.1072G>T p.E358* EX3E 27.4% nonsensePE728 FAT1 c.11690G>C p.G3897A EX22 1.9% missensePE717 CTNNA1 c.1387C>T p.Q463* EX10 2.8% nonsensePE754 RB1 c.1332G>C p.Q444H EX13 0.5% missensePE791 CDKN1A c.257G>A p.R86Q EX3 0.7% missensePE651 BRAF c.1799T>A p.V600E EX15 7.6% missensePE840 TP53 c.434T>C p.L145P EX5 32.3% missensePE742 GRIN2A c.3065G>A p.R1022H EX13E 14.0% missensePE785 CHD6 c.788T>A p.I263N EX5 39.5% missensePE891 NTHL1 c.440C>T p.T147M EX3 3.7% missensePE895 ASXL1 c.3646G>T p.V1216L EX13E 1.6% missensePE935 NF2 c.123G>A p.W41* EX2 11.5% nonsensePE262 EGFR c.2573T>G p.L858R EX21 16.0% missensePES121 EZH2 c.1786G>A p.A596T 0.3% missense

PE424 SETD2 c.5821G>A p.E1941K EX12 1.5% missensePE472 FGFR2 c.712G>A p.G238R EX6 3.0% missensePE383 LRP1B c.708T>G p.H236Q EX6 28.3% missensePE421 FBXW7 c.715A>G p.K239E EX4 28.5% missensePE638 PIK3CA c.1633G>A p.E545K EX10 6.9% missensePE639 ERBB2 c.2584A>G p.T862A EX21 52.5% missensePE665 NTRK3 c.1925G>A p.G642E EX17 1.0% missensePE666 TSC1 c.2391+1G>T . IVS18 2.9% splice-5PE609 TP53 c.647T>A p.V216E EX6 0.3% missensePE858 AXIN2 c.1961G>A p.G654D EX8 12.3% missensePE840 LRP1B c.8156C>A p.S2719Y EX51 3.5% missensePE938 PTCH2 c.1069C>T p.R357W EX8 7.4% missensePE890 CDK12 c.185C>T p.S62F EX1 2.5% missensePE913 RICTOR c.746A>T p.E249V EX8 5.1% missensePE917 KRAS c.35G>A p.G12D EX2 37.8% missensePE249 BCOR c.4165G>A p.D1389N EX9 4.1% missensePE207 TP73 c.932A>C p.E311A EX8 9.5% missensePE317 MUTYH c.1253A>T p.K418M EX13 2.1% missensePE339 LRP1B c.3985G>A p.V1329I EX25 1.6% missensePE459 STAG2 c.2314T>C p.C772R EX24 1.2% missensePE500 DNMT3A c.2408+1G>A . IVS20 1.2% splice-5PE543 ZNF804B c.316A[6>5] p.K107Nfs*15 EX3 6.2% frameshiftPE497 EGFR c.2239_2256delTTAAGAGAAGCAACATCTinsCAAp.L747_S752delinsQEX19 27.5% cds-indelPE518 EGFR c.2369C>T p.T790M EX20 3.0% missensePE576 RNASEL c.1207C>T p.R403W EX2 10.1% missensePE762 TP53 c.637C>T p.R213* EX6 34.3% nonsensePE640 SMAD4 c.670C>T p.Q224* EX6 28.1% nonsensePE744 RBM10 c.1052C[3>2] p.L352Cfs*21 EX9 47.8% frameshiftPE806 HOXA11 c.246C>A p.C82* EX1 38.6% nonsensePE889 EGFR c.2573T>G p.L858R EX21 73.2% missensePE360 CTNNB1 c.1408C>A p.H470N EX9 0.6% missensePE658 MLL2 c.15599A>G p.H5200R EX48 0.5% missensePE906 KEAP1 c.1772G[2>3] p.S592Efs*27 EX6E 41.7% frameshiftPE914 EGFR c.2573T>G p.L858R EX21 27.7% missensePE655 BMPR1A c.1161C>G p.F387L EX10 28.1% missensePE222 TP53 c.541C>T p.R181C EX5 0.6% missensePE218 CDK8 c.480G>T p.M160I EX5 1.9% missensePE302 MAPK3 c.1027G>T p.E343* EX8 21.8% nonsensePE305 TP53 c.488A>G p.Y163C EX5 0.5% missensePE310 KRAS c.35G>C p.G12A EX2 34.2% missensePE523 PIK3R1 c.1390G>A p.D464N EX11 22.1% missensePES121 KRAS c.35G>A p.G12D EX2 15.3% missensePE421 ARID2 c.3503C>T p.S1168F EX15 10.4% missensePE512 SMO c.2288G>A p.R763Q EX12E 13.2% missensePE580 TP53 c.328_347delCGTCTGGGCTTCTTGCATTCp.R110Wfs*32 EX4 4.3% frameshiftPE588 LRP1B c.1991G>T p.W664L EX13 31.0% missensePE440 RICTOR c.2179G>A p.A727T EX23 19.1% missensePE701 LRP4 c.1087G>C p.G363R EX10 29.2% missensePE737 NF1 c.3131A>G p.Y1044C EX24 46.2% missensePE690 ABCC11 c.1757G>A p.G586E EX13 1.2% missensePE689 LRP1B c.12966C>A p.Y4322* EX84 2.6% nonsensePE700 RB1 c.440C>A p.A147D EX4 0.5% missensePE687 GNAS c.105G>T p.Q35H EX1 6.6% missensePE650 BRCA2 c.22A>G p.R8G EX2 61.0% missense

PE678 FCGR2A c.100C>G p.Q34E EX2 2.4% missensePE877 ABL2 c.2039A>G p.H680R EX12E 3.7% missensePE916 ERBB2 c.2331_2339dupGGGCTCCCCp.G778_P780dup EX20 9.8% cds-insPE900 ARID1B c.986C>G p.A329G EX1 12.1% missensePE591 MAP2K4 c.248C>T p.S83L EX3 31.9% missensePE821 TRIM58 c.598C>A p.Q200K EX3 3.7% missensePE833 MLL3 c.14065T>G p.F4689V EX54 13.7% missensePE222 ROBO2 c.272A>T p.K91M EX3 17.6% missensePE260 SMAD4 c.1539C[3>2] p.P514Qfs*23 EX12 2.9% frameshiftPE207 IRS2 c.2570G>A p.G857D EX1 9.5% missensePE339 MLH1 c.57C>G p.I19M EX1 2.1% missensePE370 PIK3CA c.329A[4>1] p.K111del EX2 2.6% cds-delPE543 MLL2 c.6901C[5>6] p.N2303Kfs*6 EX31 4.8% frameshiftPE474 BRD4 c.2989_3003delGTGCACTTGCAGCCCp.V997_P1001delEX14 21.6% cds-delPE497 CDKN2A c.35C>A p.S12* EX1 3.4% nonsensePE594 NSD1 c.3067C>T p.R1023* EX5 23.5% nonsensePE581 HDAC4 c.3182C>T p.S1061L EX26 43.8% missensePE731 HSPH1 c.2450A>G p.N817S EX18E 9.5% missensePE832 CUL3 c.552A[3>2] p.N185Mfs*6 EX5 19.3% frameshiftPE788 AXIN1 c.800T>G p.L267R EX2 1.5% missensePE715 NFE2L2 c.246A>C p.E82D EX2 4.8% missensePE790 ERBB2 c.2313_2324dupATACGTGATGGCp.Y772_A775dup EX20 7.5% cds-insPE652 TP53 c.337T>G p.F113V EX4 1.4% missensePE906 FRMPD4 c.3278G>A p.W1093* EX16 51.3% nonsensePE861 KRAS c.34G>T p.G12C EX2 0.6% missensePE209 ARID2 c.5068C>T p.H1690Y EX18 0.6% missensePE303 HSPH1 c.2509G>A p.E837K EX18 2.3% missensePE207 PALB2 c.2374T>A p.S792T EX5 10.7% missensePE317 ATM c.8776G>T p.V2926F EX60 62.2% missensePE328 SMAD4 c.296G>T p.W99L EX3 21.9% missensePE204 EP300 c.6804G>C p.Q2268H EX31 11.9% missensePE367 NOTCH1 c.436_450delTCCAACCCCTGCGCCp.S146_A150del EX4 1.3% cds-delPE485 TP53 c.725G>A p.C242Y EX7 18.2% missensePE405 RBM10 c.1500dupC p.S501Qfs*7 EX13 1.6% frameshiftPE526 DNMT3A c.1240T>G p.F414V EX10 24.4% missensePES130 JAK2 c.1849G>T p.V617F EX14 1.2% missensePE594 MYD88 c.794T>C p.L265P EX5E 17.0% missensePE561 TGFBR1 c.238C>T p.R80* EX2 51.7% nonsensePE596 NF1 c.1466A>G p.Y489C EX13 1.0% missensePE762 FOXQ1 c.476G>C p.S159T EX1E 5.4% missensePE640 EGFR c.2235_2249delGGAATTAAGAGAAGCp.E746_A750del EX19 30.8% cds-delPE716 BRIP1 c.1252G>T p.A418S EX9 14.4% missensePE743 APC c.5974C[5>4] p.P1993Lfs*51 EX16E 49.5% frameshiftPE808 KDM6A c.2185G>A p.E729K EX17 6.1% missensePE863 TP53 c.637C>T p.R213* EX6 70.8% nonsensePE807 TP53 c.722C>G p.S241C EX7 41.7% missensePE653 EGFR c.2236_2250delGAATTAAGAGAAGCAp.E746_A750del EX19 21.3% cds-delPE908 EGFR c.2236_2250delGAATTAAGAGAAGCAp.E746_A750del EX19 0.7% cds-delPE249 CDKN1A c.166G>A p.E56K EX3 5.7% missensePE207 NBN c.37G>T p.G13* EX1 9.6% nonsensePE317 CTNNB1 c.110C>T p.S37F EX3 2.8% missensePE339 LRP1B c.9793C>T p.H3265Y EX61 2.3% missensePE459 RBM10 c.1081G>T p.E361* EX9 18.4% nonsensePE543 FGFR3 c.492_493delGGinsCTp.A165S EX5 5.2% missense

PE594 MSH3 c.3181A>G p.R1061G EX23 30.4% missensePE762 MLL2 c.1934C>A p.S645Y EX10 2.6% missensePE814 HDAC4 c.1379G>A p.R460H EX12 0.4% missensePE806 NTRK1 c.2201C>A p.T734K EX16 36.3% missensePE790 TP53 c.574C>T p.Q192* EX6 12.6% nonsensePE360 TRIM58 c.595C>T p.R199W EX3 2.7% missensePE653 AURKA c.166C>T p.R56C EX3 13.0% missensePE658 ARID2 c.1246A>T p.I416F EX10 0.6% missensePE928 EGFR c.2573T>G p.L858R EX21 17.1% missensePE906 STK11 c.843delG p.L282Sfs*5 EX6 37.3% frameshiftPE861 ARID1B c.5789C>T p.P1930L EX20E 0.7% missensePE914 EGFR c.324A>T p.R108S EX3 27.3% missensePE252 SMAD4 c.1000C>T p.Q334* EX9 5.6% nonsensePE264 EGFR c.2236_2251delGAATTAAGAGAAGCAAinsCAAGp.E746_T751delinsQAEX19 2.8% cds-indelPE335 PIK3CA c.1633G>A p.E545K EX10 2.4% missensePE378 FGFR1 c.441C>A p.N147K EX4 6.0% missensePE364 CIC c.1009G>C p.G337R EX7 16.7% missensePE477 ROBO3 c.1198C>A p.R400S EX8 26.2% missensePE489 EGFR c.2126A>T p.E709V EX18 2.8% missensePE418 KDM6A c.733G>T p.V245F EX9 7.1% missensePE411 FOXA1 c.244G>A p.V82I EX2E 4.5% missensePE586 CTNNB1 c.1349C>T p.T450I EX9 33.4% missensePE643 PIK3CA c.1258T>C p.C420R EX8 10.0% missensePE689 PALB2 c.2143G>A p.D715N EX5 1.3% missensePE648 CARD11 c.536G>A p.R179Q EX5 4.9% missensePE680 SEC31A c.3157C>T p.P1053S EX24 9.6% missensePE722 JARID2 c.2968C>G p.L990V EX14 18.1% missensePE765 ROS1 c.5816C>A p.A1939D EX36 0.5% missensePE809 EGFR c.2573T>G p.L858R EX21 3.3% missensePE759 CIC c.2726C>T p.P909L EX11 2.8% missensePE888 TP53 c.406C>G p.Q136E EX5 21.4% missensePE703 ATRX c.4957-2A>T . IVS18 74.3% splice-3PE886 WRN c.2694G>C p.K898N EX22 3.6% missensePE863 BRD2 c.1474G>C p.E492Q EX8 10.2% missensePE592 ATM c.2330G>A p.R777K EX15 5.9% missensePE907 RB1 c.518_519insAATAp.Y173*fs*1 EX5 69.2% frameshiftPE816 TOP2A c.4030G>T p.D1344Y EX31 2.1% missensePE325 RBM10 c.1271_1285delTGCTGCAGATCCTGCp.L424_L428del EX11 10.7% cds-delPE336 BCL3 c.1315C>G p.R439G EX9 20.1% missensePE208 ROS1 c.3017A>G p.E1006G EX20 0.4% missensePE403 ERBB4 c.1546G>T p.G516W EX13 3.9% missensePE429 KDM5A c.3089_3103delGACGCCCTATTCCTGp.G1030_P1034delEX21 15.7% cds-delPE472 TAL1 c.926C>A p.A309E EX4E 8.0% missensePE417 FAT1 c.10381C>A p.Q3461K EX18 9.6% missensePE473 HIST1H3B c.52C>A p.R18S EX1E 16.3% missensePE601 EGFR c.2369C>T p.T790M EX20 1.8% missensePE531 NPM1 c.486A>T p.E162D EX6 3.3% missensePE528 RBM10 c.1912C>T p.Q638* EX16 1.9% nonsensePE594 GNAS c.718G>A p.D240N EX9 19.3% missensePE505 EGFR c.2573T>G p.L858R EX21 68.9% missensePE572 SLC22A2 c.1174T>A p.F392I EX7 41.5% missensePE702 SPSB4 c.535G>A p.V179M EX2 6.8% missensePE599 EPHA2 c.445G>T p.E149* EX3 75.0% nonsensePE480 CREBBP c.2834C>T p.S945L EX14 8.5% missense

PE749 TBX3 c.1398G>T p.K466N EX7 9.7% missensePE774 PLK1 c.643C>G p.P215A EX3 3.8% missensePE798 HIST1H4I c.106C>T p.R36W EX1E 1.0% missensePE882 EGFR c.2573T>G p.L858R EX21 5.0% missensePE239 PDGFRB c.17C>T p.A6V EX2 0.6% missensePE292 TP53 c.413C[2>1] p.K139Rfs*31 EX5 1.9% frameshiftPE293 EGFR c.2369C>T p.T790M EX20 9.9% missensePE362 INPP4B c.1109C>A p.A370D EX15 1.5% missensePE356 FGFR3 c.991T[3>4] p.E332*fs*1 EX8 42.8% frameshiftPE346 SMAD2 c.1279C>T p.R427* EX10 1.0% nonsensePE462 BRAF c.1397G>T p.G466V EX11 1.5% missensePE448 CREBBP c.1739C>G p.A580G EX8 1.2% missensePE584 APC c.6896C>T p.P2299L EX16E 4.0% missensePE635 ARID1A c.4477C>T p.Q1493* EX18 8.5% nonsensePE484 EGFR c.2389T>G p.C797G EX20 6.3% missensePE705 FAS c.639A[2>3] p.T214Nfs*5 EX7 1.1% frameshiftPE804 APC c.1492A>T p.R498* EX12 18.4% nonsensePE657 ZFHX3 c.7927C>T p.R2643C EX9 4.2% missensePE714 EGFR c.2240_2257delTAAGAGAAGCAACATCTCp.L747_P753delinsSEX19 21.2% cds-indelPE878 CARD11 c.79G>C p.E27Q EX3 3.6% missensePE891 FAT2 c.6565_6566delCCinsAAp.P2189K EX9 2.7% missensePE592 EP300 c.2482C>A p.H828N EX14 6.9% missensePE220 RB1 c.1762delT p.S588Lfs*23 EX18 20.5% frameshiftPE222 EGFR c.2235_2249delGGAATTAAGAGAAGCp.E746_A750del EX19 0.2% cds-delPE325 EGFR c.2573T>G p.L858R EX21 87.2% missensePE336 STAG2 c.891C>A p.Y297* EX10 20.8% nonsensePE324 BRAF c.1799T>A p.V600E EX15 22.8% missensePE208 EP300 c.7127T>C p.L2376P EX31 1.4% missensePE363 MLL c.10048C>G p.Q3350E EX27 5.0% missensePE322 NOTCH2 c.3451G>A p.D1151N EX21 1.4% missensePE429 MYC c.482C>T p.S161L EX2 39.0% missensePE461 MPL c.329G>C p.W110S EX3 2.3% missensePE428 KEAP1 c.1532-1G>C . IVS4 4.2% splice-3PE417 ERBB4 c.2426A>T p.H809L EX20 48.8% missensePE555 TCF7L2 c.155C>G p.S52* EX1 3.2% nonsensePE524 PCSK7 c.1574T>G p.L525R EX13 8.1% missensePE520 TP53 c.1016delA p.E339Gfs*6 EX10 33.9% frameshiftPE531 SETD2 c.2987C>G p.S996* EX3 17.8% nonsensePE594 BRCA1 c.3662A>C p.E1221A EX10 17.9% missensePE561 EP300 c.304C>T p.Q102* EX2 10.2% nonsensePE509 TP53 c.567_574delCCCTCCTCp.P190Afs*16 EX6 50.5% frameshiftPE613 FAT2 c.10018A>G p.I3340V EX14 3.4% missensePE601 STAG2 c.2673+1G>T . IVS26 1.0% splice-5PE480 EGFR c.2236_2250delGAATTAAGAGAAGCAp.E746_A750del EX19 13.3% cds-delPE783 MAX c.289C>T p.Q97* EX4 33.3% nonsensePE749 RET c.1789G>T p.G597W EX10 7.8% missensePE774 PMS2 c.-87-u287G>A . . 3.1% promoterPE857 IDH2 c.485C>T p.P162L EX4 29.1% missensePE798 IL6ST c.917G>T p.G306V EX8 2.2% missensePE828 GRIN2A c.331C>A p.Q111K EX2 68.2% missensePE905 SETD2 c.6301dupA p.T2101Nfs*5 EX15 9.0% frameshiftPE206 EGFR c.2235_2249delGGAATTAAGAGAAGCp.E746_A750del EX19 2.1% cds-delPE482 ARAP3 c.1537G>A p.D513N EX10 1.0% missensePES121 PTPRD c.757G>A p.V253I EX20 0.3% missense

PE520 EGFR c.2390G>C p.C797S EX20 5.3% missensePE576 PTEN c.551A>G p.N184S EX6 56.2% missensePE625 OR2T4 c.806C>A p.A269D EX1E 3.4% missensePE671 EGFR c.2572_2573delCTinsAGp.L858R EX21 82.9% missensePE726 EGFR c.2573T>G p.L858R EX21 16.0% missensePE700 HDAC4 c.1488G>T p.Q496H EX12 0.5% missensePE471 TP53 c.814G>T p.V272L EX8 5.5% missensePE761 AKT3 c.1349A[5>4] p.K451Nfs*55 EX12 18.4% frameshiftPE741 SMAD4 c.431C>A p.S144* EX4 4.6% nonsensePE619 DNMT3A c.1634A>G p.E545G EX14 2.2% missensePE816 FANCA c.1226-1G>A . IVS13 1.6% splice-3PE491 NF1 c.1907C>G p.S636C EX17 58.0% missensePE927 DNMT3A c.428G>A p.R143Q EX4 1.4% missensePE905 SMAD4 c.1538_1539insT p.R515Kfs*12 EX12E 10.1% frameshiftPE872 ERBB4 c.1618G>A p.D540N EX13 22.7% missensePE913 KDM6A c.1285G>T p.A429S EX13 39.8% missensePE262 MTOR c.5506G>A p.A1836T EX39 1.0% missensePE343 EGFR c.2573T>G p.L858R EX21 14.7% missensePE383 CTNNB1 c.136C>G p.L46V EX3 43.4% missensePES121 CSF1R c.1024T>C p.F342L EX7 0.3% missensePE531 PAK3 c.525T>A p.D175E EX9 1.8% missensePE581 CDH23 c.2151_2152delCCinsAAp.Q718K EX20 32.1% missensePE746 BCOR c.1448C>T p.P483L EX4 19.5% missensePE741 DDR2 c.821A>G p.E274G EX8 7.5% missensePE666 TSC1 c.1180_1189delTCTCCTCCTCp.S394Qfs*43 EX12 2.3% frameshiftPE687 LRP1B c.3198delC p.C1066Wfs*113 EX21 2.4% frameshiftPE758 NF1 c.2407C>T p.Q803* EX20 16.3% nonsensePE840 IGF1 c.262C>T p.Q88* EX3 2.1% nonsensePE858 ASXL1 c.4390C>T p.P1464S EX13E 17.7% missensePE840 MET c.3027_3028delAGp.D1010Sfs*4 EX14 7.2% frameshiftPE911 MITF c.1180C>G p.R394G EX10E 2.8% missensePE801 IRS2 c.2030C>T p.P677L EX1 12.7% missensePE938 EGFR c.2240_2257delTAAGAGAAGCAACATCTCp.L747_P753delinsSEX19 9.0% cds-indelPE801 FLT3 c.22G>C p.G8R EX1 20.1% missensePE917 VEZF1 c.1496A>G p.N499S EX6E 17.3% missensePE930 ELMO1 c.1579G>A p.D527N EX17 1.0% missensePES130 TP53 c.731G_732CdelinsTp.G244Vfs*3 EX7 7.4% frameshiftPE222 MIB1 c.895C>T p.P299S EX6 7.7% missensePE212 RBM10 c.2082dupA p.G695Rfs*43 EX17 15.1% frameshiftPE222 AKT3 c.1054G>A p.E352K EX10 0.8% missensePE260 SMARCA4 c.3306C>G p.F1102L EX24 2.1% missensePE207 CASQ2 c.1019A>G p.D340G EX11 8.6% missensePE339 DEK c.776C>G p.T259R EX8 2.6% missensePE320 FAT1 c.8087C>T p.P2696L EX10 1.1% missensePE370 MLL3 c.5335G>T p.E1779* EX36 1.2% nonsensePE492 EGFR c.865G>A p.A289T EX7 13.5% missensePE594 EGFR c.2316_2317insTACAACCCCp.P772_H773insYNPEX20 7.7% cds-insPE624 TP53 c.499C>A p.Q167K EX5 2.4% missensePE731 NOTCH3 c.6133C>T p.P2045S EX33E 14.6% missensePE764 SETD2 c.7217A>G p.Y2406C EX17 32.7% missensePE777 ARAP3 c.1537G>A p.D513N EX10 1.1% missensePE832 KDM5A c.1503G>T p.K501N EX12 5.5% missensePE620 MLL3 c.13166C[3>2] p.K4391Nfs*22 EX52 9.3% frameshiftPE875 LRP1B c.6146A>T p.Y2049F EX38 4.5% missense

PE360 BRCA2 c.3525G>T p.Q1175H EX11 0.6% missensePE912 GMPS c.1528T>G p.F510V EX12 27.4% missensePE796 TP53 c.718A>C p.S240R EX7 52.4% missensePE923 RET c.1566_1567delCAp.S522Rfs*7 EX8 2.1% frameshiftPE258 EGFR c.2240_2257delTAAGAGAAGCAACATCTCp.L747_P753delinsSEX19 6.1% cds-indelPE296 KEAP1 c.998G>A p.G333D EX3 1.9% missensePE319 TP53 c.329G>T p.R110L EX4 83.0% missensePE376 ARID1A c.4427delT p.M1476Sfs*5 EX18 3.9% frameshiftPE424 CCND1 c.122C>T p.S41L EX1 1.2% missensePE364 LRP1B c.2239C>G p.H747D EX14 12.0% missensePE457 ATR c.1391G>T p.S464I EX6 1.2% missensePE523 BRD4 c.3367G>A p.E1123K EX16 6.6% missensePE421 STAT3 c.1392C>G p.I464M EX16 8.2% missensePE512 TP53 c.863_872delATCTCCGCAAp.N288Rfs*54 EX8 36.6% frameshiftPE588 PTPRD c.2971G>T p.D991Y EX28 23.8% missensePE553 FAM5C c.1329G[3>2] p.G444Efs*7 EX8E 41.8% frameshiftPE440 MLL2 c.2753C[4>3] p.P919Hfs*11 EX10 29.7% frameshiftPE689 ATR c.616G>C p.E206Q EX4 1.0% missensePE698 FAT2 c.11888C>G p.S3963* EX20 1.8% nonsensePE877 HIST1H2BJc.71A>G p.K24R EX1E 3.3% missensePE902 EGFR c.2573T>G p.L858R EX21 4.0% missensePE826 TP53 c.722C[2>1] p.C242Afs*5 EX7 83.6% frameshiftPE900 MCL1 c.367G>A p.E123K EX1 1.5% missensePE821 EPHB6 c.1747G>T p.G583W EX11 2.2% missensePE632 PDCD1LG2 c.686T>G p.F229C EX5 1.0% missensePE931 EGFR c.2369C>T p.T790M EX20 1.7% missensePE292 FUBP1 c.1415C>T p.P472L EX15 2.6% missensePE293 EGFR c.2573T>G p.L858R EX21 54.8% missensePE362 CUL3 c.1225G>T p.E409* EX9 1.3% nonsensePE356 FOXL2 c.352G>A p.E118K EX1 18.1% missensePE346 TP53 c.298C>T p.Q100* EX4 5.9% nonsensePE460 PIK3CA c.1034A>T p.N345I EX5 11.8% missensePE464 PIK3R2 c.811G>A p.D271N EX6 1.5% missensePE462 PARK2 c.1229C>A p.T410N EX11 1.7% missensePE358 BTK c.1387G>A p.V463I EX15 5.3% missensePE540 CYP2D6 c.501_502delCTinsAGp.H167_S168delinsQAEX3 10.9% missensePE719 TP53 c.614A>G p.Y205C EX6 16.7% missensePE610 ALK c.1020C>A p.S340R EX4 12.3% missensePE484 EGFR c.2369C>T p.T790M EX20 6.3% missensePE689 STAG2 c.1606C>T p.H536Y EX17 3.0% missensePE641 VIM c.1081delA p.T361Lfs*12 EX7 0.7% frameshiftPE804 TET2 c.4445delT p.L1482Rfs*89 EX10 1.7% frameshiftPE657 CTCF c.968A>T p.K323M EX5 9.4% missensePE867 MAPK3 c.235C>G p.Q79E EX2 10.5% missensePE878 ICK c.985C>G p.P329A EX10 2.2% missensePE915 TNFRSF14 c.817_829delATACCCTCATTCAp.I273Rfs*25 EX8E 15.5% frameshiftPE891 FAT2 c.9628G>T p.G3210C EX13 1.2% missensePE895 MAP3K1 c.1845dupT p.G616Wfs*30 EX10 7.2% frameshiftPE934 JAK3 c.1258C>A p.P420T EX10 12.7% missensePE592 AXL c.1777G>T p.D593Y EX15 4.9% missensePE400 TP53 c.844C>T p.R282W EX8 21.9% missensePE433 KDM5C c.189delC p.F63Lfs*10 EX2 11.7% frameshiftPE323 CBLB c.1235G>A p.R412H EX10 29.4% missensePE457 CDKN2A c.250G>T p.D84Y EX2 4.8% missense

PE544 EGFR c.2270A>T p.K757M EX19 85.2% missensePE552 EGFR c.2573T>G p.L858R EX21 85.7% missensePE588 EPOR c.1523C>G p.S508C EX8E 31.1% missensePE646 EGFR c.2237_2253delAATTAAGAGAAGCAACAinsTTGCTp.E746_T751delinsVAEX19 3.2% cds-indelPE669 PDGFRA c.2389A>G p.S797G EX17 4.7% missensePE763 FAM5C c.1285C>T p.P429S EX8E 6.7% missensePE689 NOTCH4 c.178G>A p.E60K EX3 1.4% missensePE729 EGFR c.2369C>T p.T790M EX20 2.0% missensePE793 PBRM1 c.3049G>T p.E1017* EX21 13.4% nonsensePE563 TP53 c.796G>T p.G266* EX8 71.5% nonsensePE885 PIK3CG c.989C>T p.T330M EX2 7.3% missensePE910 EGFR c.734A>T p.E245V EX6 1.0% missensePE935 EGFR c.2236_2250delGAATTAAGAGAAGCAp.E746_A750del EX19 27.3% cds-delPES121 BRD3 c.146C>T p.T49M 0.7% missensePE322 ROS1 c.1025G>T p.G342V EX10 37.8% missensePE424 HDAC4 c.1759G>A p.E587K EX13 1.1% missensePE374 TP53 c.376-2A>G . IVS4 3.3% splice-3PE472 GPR124 c.2029C>A p.P677T EX13 6.9% missensePE467 EGFR c.2303_2311dupGCGTGGACAp.S768_D770dup EX20 4.2% cds-insPE441 PDGFRB c.3136_3137+9delAGGTAACCACC. EX22-IVS228.2% spanPE397 CBL c.1167G>C p.K389N EX8 0.6% missensePE421 ERBB4 c.298G>A p.E100K EX3 13.7% missensePE544 MSH3 c.1780C>T p.L594F EX13 8.8% missensePE638 CTNNB1 c.110C>T p.S37F EX3 6.4% missensePE572 BRCA2 c.2864A>G p.N955S EX11 18.6% missensePE599 RBM10 c.2863-2A>G . IVS23 74.8% splice-3PE683 CDKN2A c.238C>T p.R80* EX2 32.9% nonsensePE666 EGFR c.2573T>G p.L858R EX21 33.5% missensePE758 CDKN2A c.83_100delTGCGGGCGCTGCTGGAGGp.V28_E33del EX1 9.9% cds-delPE774 CIC c.2726C>T p.P909L EX11 5.0% missensePE858 TP53 c.818G>T p.R273L EX8 12.3% missensePE703 LRP1B c.7073T>C p.V2358A EX43 22.9% missensePE913 RICTOR c.1054A>T p.S352C EX12 15.1% missensePE917 MLL3 c.2870A>T p.Q957L EX17 22.7% missensePE438 PTEN c.349A[2>1] p.N117Ifs*17 EX5 2.2% frameshiftPE389 YBX1 c.466_488delCGCAATTACCAGCAAAATTACCAp.R156Efs*2 EX5 0.5% frameshiftPES121 MLL2 c.5713C>G p.Q1905E EX26 3.6% missensePES121 MLL2 c.5713C>G p.Q1905E EX26 3.7% missensePE520 EGFR c.2235_2249delGGAATTAAGAGAAGCp.E746_A750del EX19 13.4% cds-delPE638 FZD1 c.1409G>A p.G470D EX1E 2.7% missensePE687 FAT2 c.3281C>G p.P1094R EX2 1.9% missensePE755 TP53 c.582_583insG p.I195Dfs*14 EX6 1.0% frameshiftPE816 KRAS c.34G>T p.G12C EX2 17.9% missensePE734 TP53 c.528C>A p.C176* EX5 0.6% nonsensePE616 PCK1 c.1051A>T p.I351F EX7 0.7% missensePE909 SMARCA4 c.2644G>C p.E882Q EX19 9.5% missensePE905 FLCN c.247G>A p.E83K EX4 1.2% missensePE913 NAV3 c.1245T>A p.D415E EX8 1.9% missensePE222 IGF1R c.-50-u6_-49delTTTTTTTT. .-EX1 4.0% spanPE264 NTRK3 c.1789C>T p.H597Y EX16 2.0% missensePE342 NFE2L2 c.80A>G p.D27G EX2 19.4% missensePE378 BRCA2 c.8554G>A p.A2852T EX20 3.3% missensePE404 PBRM1 c.2994delC p.Y998*fs*1 EX20 8.9% frameshiftPE464 TP53 c.814G>A p.V272M EX8 12.8% missense

PE364 BTK c.1529A>C p.E510A EX15 15.4% missensePE489 EGFR c.2369C>T p.T790M EX20 2.2% missensePE594 CASP8 c.448C>T p.Q150* EX2 0.5% nonsensePE411 GRIN2A c.394T>A p.S132T EX2 15.2% missensePE647 SETD2 c.2623G[3>4] p.S876Efs*40 EX3 16.9% frameshiftPE689 TP53 c.297_303delCCAGAAAp.Q100Pfs*21 EX4 3.1% frameshiftPE761 EGFR c.2239_2251delTTAAGAGAAGCAAinsCp.L747_T751delinsPEX19 29.0% cds-indelPE682 EGFR c.2573T>G p.L858R EX21 66.3% missensePE722 MLL2 c.9308C>G p.A3103G EX34 29.9% missensePE809 TP53 c.584T>C p.I195T EX6 9.0% missensePE725 NFKBIA c.764A>T p.Q255L EX5 31.8% missensePE866 WT1 c.956C>T p.A319V EX5 7.4% missensePE910 ERCC5 c.2698C>G p.Q900E EX13 3.4% missensePE886 PPM1D c.1617_1618insTAATp.E540*fs*1 EX6E 1.2% frameshiftPE863 OR4A15 c.499C>G p.R167G EX1E 54.9% missensePE891 EPHA2 c.1349C>T p.T450I EX6 3.1% missensePE934 EGFR c.2236_2250delGAATTAAGAGAAGCAp.E746_A750del EX19 8.7% cds-delPE592 BRCA2 c.7559G>A p.R2520Q EX15 3.9% missensePE816 RBM10 c.1117A>C p.N373H EX10 1.7% missensePE918 EGFR c.2237_2251delAATTAAGAGAAGCAAp.E746_T751delinsAEX19 19.0% cds-indelPE273 TP53 c.818G>A p.R273H EX8 1.3% missensePE248 EGFR c.2235_2249delGGAATTAAGAGAAGCp.E746_A750del EX19 4.4% cds-delPE523 TP53 c.858A>T p.E286D EX8 30.1% missensePE613 RB1 c.644C>A p.S215* EX7 44.2% nonsensePE737 FLT1 c.2026A>T p.S676C EX14 1.5% missensePE726 TP53 c.632delC p.T211Ifs*36 EX6 33.5% frameshiftPE779 PIK3CA c.1624G>A p.E542K EX10 1.3% missensePE650 EGFR c.2235_2249delGGAATTAAGAGAAGCp.E746_A750del EX19 22.5% cds-delPE748 RET c.1789G>T p.G597W EX10 7.2% missensePE872 ERBB2 c.1169C>G p.A390G EX10 13.5% missensePE801 NF2 c.123G>A p.W41* EX2 19.0% nonsensePE297 EGFR c.2389T>A p.C797S EX20 15.1% missensePE309 KRAS c.34G>T p.G12C EX2 54.7% missensePE207 CREBBP c.4990C>T p.R1664C EX30 20.1% missensePE394 PAK3 c.534AGA[5>4] p.E176[7>6] EX9 17.4% cds-delPE503 PIK3CA c.1624G>A p.E542K EX10 10.7% missensePE546 IMPG1 c.122G>A p.R41K EX2 1.7% missensePE554 PIK3CA c.341A>G p.N114S EX2 2.5% missensePE214 HDAC4 c.1168G>A p.G390S EX11 6.4% missensePE318 TP53 c.378C>G p.Y126* EX5 36.8% nonsensePE593 CYP1A1 c.1364G>A p.R455Q EX7E 6.7% missensePE664 FH c.390TAAAT[2>1] p.L132*fs*1 EX4 3.2% frameshiftPE586 EGFR c.2235_2249delGGAATTAAGAGAAGCp.E746_A750del EX19 41.7% cds-delPE654 TP53 c.1006G>T p.E336* EX10 75.5% nonsensePE728 MLL2 c.178G[5>4] p.G61Vfs*69 EX3 7.9% frameshiftPE760 PBRM1 c.3567_3577delAGTATTTCTGAp.V1190*fs*1 EX23 70.2% frameshiftPE385 WRN c.3857C>T p.T1286I EX33 1.1% missensePE694 ARID1A c.6161delA p.E2054Gfs*81 EX20E 34.4% frameshiftPE649 NBN c.2084G>C p.G695A EX14 11.3% missensePE788 EGFR c.2326C>T p.R776C EX20 6.0% missensePE686 PHF6 c.1039C>T p.R347* EX10 10.3% nonsensePE565 RBM10 c.2002C>T p.Q668* EX17 16.2% nonsensePE564 EGFR c.2573T>G p.L858R EX21 35.0% missensePE792 SDHB c.661G>T p.D221Y EX7 4.3% missense

PE800 TERT c.-58-u66C>T . . 8.4% promoterPE876 HIST1H2BJc.71A>G p.K24R EX1E 1.0% missensePE899 EGFR c.2369C>T p.T790M EX20 0.7% missensePE299 TGFBR2 c.1223_1247delCCAATATCCTCGTGAAGAACGACCTp.S408*fs*1 EX5 7.9% frameshiftPE315 TP53 c.814G>A p.V272M EX8 5.7% missensePE207 FGFR2 c.2T>C p.0? EX2 10.1% init-lossPE317 H3F3C c.265G>T p.V89F EX1 16.5% missensePE374 TP53 c.699_701delCTAinsGTGp.H233_Y234delinsQCEX7 3.9% missensePE459 TP53 c.385_389delGCCCTp.A129Qfs*18 EX5 1.5% frameshiftPE428 EGFR c.2573T>G p.L858R EX21 3.5% missensePE489 NF1 c.1454A>G p.E485G EX13 3.1% missensePE526 EGFR c.2237_2256delAATTAAGAGAAGCAACATCTinsTAp.E746_S752delinsVEX19 26.7% cds-indelPE543 MECOM c.1466T>G p.F489C EX8 2.7% missensePE594 IL7R c.1184A>C p.K395T EX8E 18.9% missensePE561 ATM c.3192G[3>2] p.G1065Efs*6 EX22 3.7% frameshiftPE732 EP300 c.4852C>G p.L1618V EX30 2.2% missensePE696 FAT1 c.11866A>G p.I3956V EX22 0.6% missensePE761 TP53 c.843C>A p.D281E EX8 49.2% missensePE355 TP53 c.808T>G p.F270V EX8 1.9% missensePE744 TP53 c.827C>A p.A276D EX8 21.8% missensePE679 NOD1 c.27G>A p.M9I EX4 1.4% missensePE812 TP53 c.820G>T p.V274F EX8 76.2% missensePE788 ZNF804B c.367C>T p.Q123* EX3 1.6% nonsensePE743 EGFR c.2369C>T p.T790M EX20 11.8% missensePE778 EGFR c.2573T>G p.L858R EX21 9.9% missensePE360 DDR2 c.989G>T p.S330I EX9 2.7% missensePE901 NF1 c.1549G>A p.E517K EX14 1.1% missensePE906 KRAS c.37G>T p.G13C EX2 36.3% missensePE291 TGFBR2 c.1558C>T p.R520* EX7 2.6% nonsensePE273 TLR4 c.2288G>A p.R763H EX3 1.9% missensePE322 TP53 c.488A>G p.Y163C EX5 84.2% missensePE424 EGFR c.2573T>G p.L858R EX21 7.3% missensePE472 EP300 c.1119G>C p.K373N EX4 2.5% missensePE523 FAT2 c.8002C>A p.P2668T EX9 30.7% missensePE638 PPM1D c.1618G>C p.E540Q EX6E 3.0% missensePE559 MAP2K2 c.391G>A p.V131M EX3 0.3% missensePE737 FLT3 c.1055C>A p.A352D EX9 38.9% missensePE687 TP53 c.329_331delGTC p.R110del EX4 2.1% cds-delPE748 CDKN2A c.298_313delGCCGGGGCGCGGCTGGp.A100Tfs*41 EX2 5.5% frameshiftPE810 EPHA5 c.2789A>G p.N930S EX16 0.6% missensePE840 GRM3 c.1092G>C p.Q364H EX3 0.4% missensePE937 HOXC13 c.838G>A p.A280T EX2E 2.3% missensePE210 TP53 c.523C>G p.R175G EX5 14.6% missensePE223 FAM5C c.1330G>T p.G444* EX8 63.5% nonsensePE267 KRAS c.35G>T p.G12V EX2 47.7% missensePE260 BRIP1 c.2894G>A p.R965K EX19 0.5% missensePE263 FAT1 c.11120C>G p.S3707* EX19 11.7% nonsensePE345 CCND1 c.593A>G p.N198S EX4 48.8% missensePE207 FH c.169T>C p.F57L EX2 5.1% missensePE279 EGFR c.2369C>T p.T790M EX20 1.1% missensePE313 MET c.3028+1G>A . IVS14 28.8% splice-5PE339 MLL c.6027G>C p.L2009F EX23 2.0% missensePE320 TP53 c.889_911delCACGAGCTGCCCCCAGGGAGCACp.H297*fs*1 EX8 12.3% frameshiftPE379 MET c.3028delG p.D1010Ifs*19 EX14 25.3% frameshift

PE429 TRIM58 c.691G>C p.E231Q EX3 26.5% missensePE454 ARID2 c.1580+1G>T . IVS12 91.4% splice-5PE428 PIK3C2G c.4180G>A p.E1394K EX31 1.4% missensePE569 TP53 c.363TG[2>1] p.V122Dfs*26 EX4 0.7% frameshiftPE594 BRD3 c.207C>G p.N69K EX2 2.3% missensePE639 ALK c.2041+1G>C . IVS11 2.5% splice-5PE764 SMAD4 c.477G>C p.K159N EX5 10.6% missensePE718 TP53 c.919+1G>T . IVS8 1.3% splice-5PE840 ARID1A c.112G>A p.E38K EX1 2.3% missensePE863 AKT2 c.1199C>T p.A400V EX12 1.1% missensePE898 TP53 c.332T>G p.L111R EX4 51.5% missensePE875 C1S c.391+2T>C . IVS4 5.7% splice-5PE360 ASXL1 c.305C>T p.P102L EX5 6.1% missensePE631 FCGR2B c.773A>G p.Y258C EX6 3.2% missensePE828 LRP1B c.939C>A p.N313K EX7 4.0% missensePE801 PBRM1 c.3724C>T p.Q1242* EX24 25.1% nonsensePE693 EGFR c.2236_2250delGAATTAAGAGAAGCAp.E746_A750del EX19 14.7% cds-delPE275 TOP2A c.3184A>G p.K1062E EX24 13.9% missensePE436 MET c.3583C>T p.L1195F EX18 4.9% missensePE429 BCOR c.4082G>A p.R1361K EX9 13.8% missensePE374 DNMT3A c.2645G>T p.R882L EX23E 0.5% missensePE448 EGFR c.2236_2250delGAATTAAGAGAAGCAp.E746_A750del EX19 34.0% cds-delPE472 PIK3CG c.569G>T p.S190I EX2 6.0% missensePE607 BRD2 c.1221_1222insA p.Y408Ifs*10 EX7 7.2% frameshiftPE417 GRIN2A c.3052G>A p.V1018M EX13E 38.4% missensePE473 MN1 c.1595_1596insACAp.Q523[28>29] EX1 4.0% cds-insPE549 TP53 c.592G>T p.E198* EX6 9.4% nonsensePE439 EGFR c.2170G>A p.G724S EX18 15.5% missensePE397 SLIT2 c.1778G>A p.R593H EX18 0.8% missensePE528 ARID1A c.3635_3638delAGCCp.Q1212Pfs*3 EX14 8.2% frameshiftPE672 MLL3 c.9254C>G p.P3085R EX38 1.7% missensePE572 PAK1 c.710G>A p.R237Q EX7 21.5% missensePE704 HOXC13 c.838G>A p.A280T EX2E 2.4% missensePE690 FAT2 c.12281A>T p.D4094V EX22 1.4% missensePE723 BCOR c.4165G>A p.D1389N EX9 3.0% missensePE781 SMAD4 c.1081C>T p.R361C EX9 35.5% missensePE840 FGF4 c.450C>A p.F150L EX3E 1.2% missensePE858 WSCD2 c.629C>A p.A210D EX4 9.3% missensePE884 ZFHX3 c.547G>A p.D183N EX2 32.7% missensePE801 ATR c.5504C>G p.S1835* EX32 3.4% nonsensePE913 PBRM1 c.1413G>C p.K471N EX13 17.2% missensePE269 EPAS1 c.622C>T p.H208Y EX6 1.1% missensePE274 FAT1 c.7418G>C p.R2473T EX10 6.8% missensePE257 EGFR c.2240_2257delTAAGAGAAGCAACATCTCp.L747_P753delinsSEX19 49.5% cds-indelPE252 MED12 c.466G>A p.A156T EX4 35.2% missensePE349 NCOR1 c.266dupA p.Y89*fs*1 EX4 13.4% frameshiftPE287 EXT1 c.169C>G p.R57G EX1 6.4% missensePE331 MLL3 c.7829G>A p.R2610Q EX38 1.4% missensePE424 ELAC2 c.904C>T p.P302S EX11 1.2% missensePE368 TP53 c.528C[5>4] p.H178Tfs*69 EX5 10.1% frameshiftPE364 TGFBR1 c.1198G>A p.D400N EX7 57.0% missensePE383 ERBB2 c.2339_2340insCGGCTCCCCp.G778_P780dup EX20 44.5% cds-insPE489 PIK3CA c.3140A>T p.H1047L EX21E 3.3% missensePE457 NOTCH3 c.5717G>T p.G1906V EX31 6.3% missense

PE578 BCOR c.4229C>A p.S1410* EX10 1.1% nonsensePE474 BRIP1 c.29T>C p.I10T EX2 3.1% missensePE510 FOXA1 c.521C>G p.S174W EX2E 35.2% missensePE733 LRP1B c.7123G>C p.E2375Q EX43 6.5% missensePE669 FLT1 c.2369_2372dupTAAAp.K791Nfs*4 EX17 7.8% frameshiftPE689 PTPRD c.2716G>A p.E906K EX28 1.5% missensePE793 TP53 c.842A>G p.D281G EX8 22.4% missensePE883 SLX4 c.1054C>T p.H352Y EX5 1.4% missensePE739 EGFR c.2236_2250delGAATTAAGAGAAGCAp.E746_A750del EX19 10.3% cds-delPE919 NSD1 c.4604G>A p.R1535H EX11 1.2% missensePES130 EGFR c.2573T>G p.L858R EX21 49.3% missensePE222 WT1 c.56G>C p.R19P EX1 11.6% missensePE211 HDAC4 c.2315G>A p.R772H EX18 16.4% missensePE222 DNMT3A c.1344C>A p.Y448* EX11 1.5% nonsensePE260 GPX1 c.415G>A p.D139N EX2 2.5% missensePE207 BRIP1 c.1686T>G p.I562M EX12 9.2% missensePE339 FAT2 c.8444C>A p.S2815* EX9 1.6% nonsensePE415 HOXC13 c.791G>A p.R264H EX2E 1.5% missensePE388 MLH1 c.2213G>A p.G738E EX19E 4.1% missensePE500 MET c.3682G>A p.D1228N EX19 4.9% missensePE518 MED12 c.4205G>A p.S1402N EX30 2.6% missensePE594 ARID1B c.553G>A p.E185K EX1 29.3% missensePE580 CTNNA1 c.1919A>C p.D640A EX14 6.3% missensePE624 TERT c.-58-u88C>T . . 38.4% promoterPE718 EPHA5 c.1824_1825delCCinsAAp.L609M EX9 1.2% missensePE771 HIST1H1C c.444G>T p.K148N EX1E 6.8% missensePE695 CREBBP c.742C>T p.P248S EX2 2.5% missensePE832 CDH23 c.1814C>T p.A605V EX17 2.8% missensePE661 PALB2 c.3397A>G p.T1133A EX13E 0.3% missensePE710 PIK3CB c.802-1G>A . IVS4 8.2% splice-3PE715 PTEN c.405dupA p.C136Mfs*44 EX5 5.0% frameshiftPE806 STK11 c.454C>T p.Q152* EX3 75.1% nonsensePE889 RUNX1 c.277G>C p.D93H EX4 7.9% missensePE620 ROS1 c.6094G>A p.G2032R EX38 7.6% missensePE360 SYK c.758G>C p.R253T EX5 1.4% missensePE887 EGFR c.2573T>G p.L858R EX21 1.2% missensePE928 TP53 c.406C>G p.Q136E EX5 18.3% missensePE858 GRM3 c.1092G>C p.Q364H EX3 0.9% missensePE207 POLE c.4529_4530delCAinsTGp.A1510V EX35 2.1% missensePE353 EGFR c.2573T>G p.L858R EX21 15.5% missensePE338 CCNE1 c.23+1_23+15delGTGAGTGCCCGCGCC. IVS2 14.6% splice-5PE339 AURKA c.152C>G p.S51* EX3 1.0% nonsensePE363 CDKN2C c.337G>A p.E113K EX2 5.7% missensePE465 GRIN2A c.2933A>T p.Y978F EX13E 19.1% missensePE468 ATM c.266C>T p.S89F EX4 26.4% missensePE417 OR6F1 c.799G>A p.A267T EX1E 7.3% missensePE555 OBSCN c.4727C>G p.S1576C EX16 3.5% missensePE520 POLE c.5179_5193delGTGGAGCTGGACCTTinsCp.V1727Pfs*53 EX39 9.0% frameshiftPE594 PALB2 c.1492G>T p.D498Y EX4 2.1% missensePE509 EGFR c.2236_2250delGAATTAAGAGAAGCAp.E746_A750del EX19 38.7% cds-delPE783 PARP1 c.2347G>C p.D783H EX17 26.7% missensePE679 AXIN1 c.82G>A p.E28K EX2 9.8% missensePE805 TET2 c.4445delT p.L1482Rfs*89 EX10 1.3% frameshiftPE749 NFE2L2 c.85G>C p.D29H EX2 4.4% missense

PE840 EPHA5 c.98C>G p.S33C EX1 2.0% missensePE849 RPTOR c.551C>T p.T184M EX5 2.0% missensePE863 MN1 c.1750G>T p.V584L EX1 3.1% missensePE857 CDK8 c.1373C>T p.S458L EX13E 29.8% missensePE616 FLT1 c.1754G>A p.R585K EX13 7.1% missensePE631 CIC c.1067C>T p.S356F EX7 4.4% missensePE828 SGCZ c.65C>A p.A22E EX2 34.7% missensePE291 ACVR1B c.718G>T p.E240* EX4 2.9% nonsensePE273 NCOR1 c.2948T>A p.I983K EX22 3.0% missensePE392 MAX c.178C>T p.R60W EX4 6.1% missensePE424 CDK13 c.872C>T p.S291L EX1 1.1% missensePE472 U2AF1 c.101C>T p.S34F EX2 3.6% missensePE523 SMARCA4 c.255G[2>1] p.D86Tfs*8 EX3 33.6% frameshiftPE587 BRCA1 c.4408G>A p.E1470K EX13 1.5% missensePE810 BCL6 c.8C>T p.S3L EX3 0.6% missensePE839 EGFR c.2239_2248delinsCp.L747_A750delinsPEX19 84.3% cds-indelPE937 C8orf34 c.962A>G p.K321R EX7 0.7% missensePES130 MAP3K1 c.3894G>A p.M1298I EX16 5.0% missensePE212 IL7R c.178G>A p.E60K EX2 1.1% missensePE268 KRAS c.35G>T p.G12V EX2 1.3% missensePE217 FOXL2 c.941C>T p.A314V EX1 5.6% missensePE260 SF3A3 c.652G>C p.E218Q EX8 0.8% missensePE207 DICER1 c.3179G>A p.R1060H EX21 8.0% missensePE279 EGFR c.2573T>G p.L858R EX21 3.7% missensePE606 POLD1 c.49C>T p.R17W EX2 25.4% missensePE339 MLL3 c.2411T>G p.L804R EX14 7.5% missensePE329 EGFR c.2127_2129delAACp.E709_T710delinsDEX18 15.5% cds-indelPE320 TSC2 c.3475C>T p.R1159W EX30 1.4% missensePE454 NTRK1 c.1330C>T p.R444W EX11 17.8% missensePE359 WNK1 c.1283C>A p.A428D EX4 14.0% missensePE569 EGFR c.185T>G p.L62R EX2 1.8% missensePE594 MET c.3028+799A>G . IVS14 18.8% intronPE764 BRAF c.1799T>A p.V600E EX15 1.0% missensePE688 MUTYH c.568G>T p.A190S EX7 0.5% missensePE608 EGFR c.2235_2249delGGAATTAAGAGAAGCp.E746_A750del EX19 24.3% cds-delPE771 ATM c.7181C>T p.S2394L EX49 18.8% missensePE832 TOP2A c.1867G>A p.E623K EX16 2.2% missensePE772 EGFR c.2573T>G p.L858R EX21 13.9% missensePE710 CNTN5 c.215C>A p.A72E EX4 16.7% missensePE620 SMARCA4 c.4759G>A p.E1587K EX33 10.6% missensePE898 EGFR c.2573T>G p.L858R EX21 60.0% missensePE875 EGFR c.2240_2257delTAAGAGAAGCAACATCTCp.L747_P753delinsSEX19 4.0% cds-indelPE923 TSC2 c.2607_2610delCGCCinsATp.F869Lfs*13 EX23 1.5% frameshiftPES130 CDKN2A c.150+1_150+2insG. IVS1 6.6% splice-5PE212 EGFR c.2236_2250delGAATTAAGAGAAGCAp.E746_A750del EX19 4.5% cds-delPE260 MLL3 c.3324-1G>C . IVS20 1.8% splice-3PE207 PIK3CG c.2427A>C p.K809N EX6 8.3% missensePE339 EGFR c.2236_2250delGAATTAAGAGAAGCAp.E746_A750del EX19 28.1% cds-delPE320 MAP3K1 c.3071A>T p.K1024M EX14 21.5% missensePE434 EGFR c.2235_2249delGGAATTAAGAGAAGCp.E746_A750del EX19 22.0% cds-delPE500 TP53 c.800G>C p.R267P EX8 6.3% missensePE492 EGFR c.2573T>G p.L858R EX21 17.4% missensePE359 ALK c.1837G>A p.A613T EX10 15.8% missensePE764 DAXX c.1532C>T p.S511L EX6 15.2% missense

PE718 NOTCH4 c.597C>A p.F199L EX4 1.0% missensePE696 SNX29 c.803C>T p.S268L EX8 5.3% missensePE679 PTEN c.733C>T p.Q245* EX7 13.5% nonsensePE710 EGFR c.2236_2250delGAATTAAGAGAAGCAp.E746_A750del EX19 48.1% cds-delPE620 CNTN5 c.131C>G p.S44C EX4 7.3% missensePE881 RBM10 c.1643C[3>2] p.L549*fs*1 EX14 5.0% frameshiftPE898 KDR c.2455C>T p.R819* EX17 1.6% nonsensePE875 EPHA3 c.2288C>T p.S763F EX13 4.6% missensePES130 MAP3K1 c.3894G>A p.M1298I EX16 0.9% missensePE308 TP53 c.853G>C p.E285Q EX8 0.7% missensePE303 ARID1A c.2896G>C p.E966Q EX10 4.5% missensePE301 ROBO3 c.473C>T p.S158L EX2 2.8% missensePE207 JAK2 c.1056+2T>C . IVS8 14.9% splice-5PE370 KCNJ5 c.451G[3>2] p.Y152Mfs*101 EX2 7.1% frameshiftPE204 FANCM c.1361AAG[2>1] p.E454[2>1] EX8 4.8% cds-delPE413 EGFR c.2573T>G p.L858R EX21 76.8% missensePE459 PTCH2 c.940G>A p.E314K EX8 8.1% missensePE493 MED12 c.2990G>C p.C997S EX22 8.0% missensePE558 EGFR c.2573T>G p.L858R EX21 84.1% missensePE570 EGFR c.2573T>G p.L858R EX21 0.5% missensePE576 EGFR c.2573T>G p.L858R EX21 91.8% missensePE513 EGFR c.2236_2250delGAATTAAGAGAAGCAp.E746_A750del EX19 48.3% cds-delPE514 RNASEL c.1428_1431delCTTGp.L477Pfs*15 EX2 12.4% frameshiftPE720 TP53 c.742C>T p.R248W EX7 40.6% missensePE642 NTRK1 c.1903G>A p.A635T EX15 5.5% missensePE732 TBX3 c.1718C>T p.A573V EX7 5.6% missensePE679 PIK3CB c.1700A>G p.E567G EX11 10.7% missensePE807 SDHA c.1099C>T p.Q367* EX9 15.4% nonsensePE653 OR6F1 c.719C>A p.T240K EX1E 27.9% missensePE922 APC c.7158G>C p.K2386N EX16E 19.7% missensePE299 TP53 c.536A>G p.H179R EX5 27.2% missensePE209 TP53 c.743G>A p.R248Q EX7 0.9% missensePE315 EGFR c.2235_2249delGGAATTAAGAGAAGCp.E746_A750del EX19 7.5% cds-delPE207 NSD1 c.1583A>G p.K528R EX5 10.4% missensePE317 EGFR c.2573T>G p.L858R EX21 38.9% missensePE328 AKT2 c.1115C>T p.T372M EX11 8.3% missensePE526 EPHA5 c.733G>A p.D245N EX3 27.0% missensePE543 SF3B1 c.302A>G p.Y101C EX4 13.7% missensePE594 EPHA5 c.2810G>A p.R937H EX16 13.1% missensePE674 KEAP1 c.1282G>A p.V428I EX3 22.6% missensePE762 CDK6 c.761T>G p.F254C EX7 2.0% missensePE784 CREBBP c.5278C>T p.Q1760* EX31E 26.4% nonsensePE744 SMAD3 c.658+1G>C . IVS5 8.2% splice-5PE812 RB1 c.1302G[3>2] p.G435Dfs*22 EX13 60.4% frameshiftPE747 ANKRD11 c.1106A>G p.K369R EX9 0.5% missensePE788 TET2 c.2440C>T p.R814C EX3 1.6% missensePE743 EGFR c.2235_2249delGGAATTAAGAGAAGCp.E746_A750del EX19 30.5% cds-delPE807 BRD4 c.1508C>T p.S503F EX8 4.2% missensePE653 PIK3CG c.1420C>G p.L474V EX2 23.8% missensePE652 EGFR c.2314_2319dupCCCCACp.P772_H773dup EX20 1.7% cds-insPE900 PSG2 c.758C>T p.S253L EX4 1.0% missensePE901 TP53 c.517G>T p.V173L EX5 4.0% missensePE906 CDKN2A c.157_158dupAT p.M53Ifs*2 EX2 30.7% frameshiftPE223 TRAF5 c.798_805delCTTACACAp.D266Efs*9 EX9 1.0% frameshift

PE222 KRAS c.35G>A p.G12D EX2 1.6% missensePE251 VIM c.1098G>T p.Q366H EX7 0.9% missensePE362 EGFR c.2236_2250delGAATTAAGAGAAGCAp.E746_A750del EX19 28.5% cds-delPE460 EGFR c.2512C>G p.L838V EX21 40.3% missensePE462 TP53 c.839G>A p.R280K EX8 1.2% missensePE500 MSH2 c.793G>T p.V265F EX5 6.1% missensePE539 LRP1B c.1522A>T p.N508Y EX10 11.6% missensePE635 MED12 c.2591C>T p.S864L EX19 7.5% missensePE728 PMS1 c.46C>G p.Q16E EX2 2.8% missensePE648 RBM10 c.289C>T p.R97* EX3 9.0% nonsensePE819 EGFR c.2369C>T p.T790M EX20 0.8% missensePE565 LRP1B c.458G>A p.C153Y EX4 1.1% missensePE804 TP53 c.920-2A>G . IVS8 25.0% splice-3PE742 PMS1 c.2780A>G p.Y927C EX13E 26.4% missensePE915 BRAF c.541C>G p.L181V EX4 10.0% missensePE891 MLL3 c.6118C>G p.P2040A EX36 4.1% missensePE935 ERBB4 c.3043G>T p.D1015Y EX25 10.6% missensePE402 ARID1A c.4330G>T p.E1444* EX18 48.3% nonsensePE376 ARAF c.1255T>C p.Y419H EX12 3.5% missensePE488 TP53 c.856G>T p.E286* EX8 0.6% nonsensePE547 NF1 c.5531C>A p.S1844Y EX37 3.9% missensePE603 TP53 c.1015G>T p.E339* EX10 0.8% nonsensePE553 PDCD1LG2 c.208C>A p.R70S EX3 40.1% missensePE690 KEAP1 c.994G>C p.G332R EX3 26.1% missensePE689 MSH3 c.1536A>T p.E512D EX10 1.1% missensePE740 PDCD1 c.814C>T p.R272W EX5E 1.3% missensePE799 GRIN2A c.155G>A p.R52Q EX2 10.5% missensePE816 EGFR c.2235_2249delGGAATTAAGAGAAGCp.E746_A750del EX19 3.3% cds-delPE863 SERPINB4 c.865A>G p.M289V EX8E 1.2% missensePE873 ASXL1 c.2113G>T p.E705* EX13E 1.1% nonsensePE877 PTEN c.737C>T p.P246L EX7 3.5% missensePE853 EGFR c.2237_2248delAATTAAGAGAAGinsCACp.E746_A750delinsAPEX19 2.4% cds-indelPE821 KEAP1 c.660C>G p.F220L EX3 3.2% missensePE903 MAP2K1 c.159T>A p.F53L EX2 6.3% missensePE210 SMARCA4 c.3563A>T p.D1188V EX26 13.1% missensePE223 TP53 c.643_647dupAGTGTp.V218Wfs*31 EX6 42.7% frameshiftPE263 MLH3 c.1567C>T p.Q523* EX2 3.5% nonsensePE345 AXL c.583C>T p.P195S EX4 47.2% missensePE207 TRRAP c.2899A>G p.I967V EX22 5.1% missensePE279 AKT3 c.1200G>T p.M400I EX11 1.1% missensePE313 KRTAP5-5 c.96_107delCTCTGGCTGTGGp.S33_G36del EX1 10.5% cds-delPE339 HNF1A c.703G>T p.E235* EX3 4.2% nonsensePE379 ACVR1B c.935A>C p.N312T EX6 3.7% missensePE429 HDAC4 c.173C>T p.S58L EX4 26.4% missensePE465 MDM2 c.583A>G p.I195V EX8 56.6% missensePE454 TP53 c.586C>T p.R196* EX6 88.9% nonsensePE548 FH c.248G>A p.G83D EX2 31.7% missensePE569 TAF15 c.1422_1445delCCGAGGAGGTGGCTATGGAGGAGAp.G454_G461[4>3]EX15 35.1% cds-delPE594 RET c.2043G>T p.Q681H EX11 7.4% missensePE596 SMARCA4 c.3574C>T p.R1192C EX26 1.3% missensePE764 SMARCA4 c.2374C>G p.L792V EX16 1.0% missensePE644 PIK3C2B c.1346A>G p.Y449C EX7 1.6% missensePE696 RBM10 c.2914G>A p.G972R EX24E 0.6% missensePE752 CBL c.1244G>T p.G415V EX9 0.9% missense

PE898 SRC c.649A>T p.I217F EX8 9.4% missensePE875 KRAS c.35G>C p.G12A EX2 8.6% missensePE360 RBM10 c.1100T>A p.I367N EX10 4.8% missensePE894 TP53 c.536A>G p.H179R EX5 7.7% missensePE828 FBXW7 c.1385C>T p.S462F EX9 70.5% missensePE801 EPHB1 c.2609G>T p.R870L EX14 14.7% missensePE693 EGFR c.2369C>T p.T790M EX20 10.9% missensePE262 MLL c.1062delGinsTAATp.R354delinsSN EX3 0.7% cds-indelPE343 TP53 c.817C>T p.R273C EX8 9.4% missensePE322 PAX5 c.339G>C p.E113D EX3 40.8% missensePE467 BCOR c.937C[3>2] p.R314Gfs*64 EX4 7.0% frameshiftPE441 EGFR c.2389T>A p.C797S EX20 11.9% missensePES121 DNMT3A c.2329C>A p.P777T EX20 0.9% missensePE638 ARAP3 c.1537G>A p.D513N EX10 1.6% missensePE572 BRIP1 c.1696G>T p.D566Y EX12 35.8% missensePE690 GALNT12 c.1018C>G p.L340V EX5 20.6% missensePE666 TSC1 c.664-5_666delTCTAGCCA. IVS7-EX8 6.5% spanPE687 ERBB4 c.3205G[2>1] p.G1069Efs*83 EX27 2.6% frameshiftPE758 CDK12 c.3083T>A p.M1028K EX11 20.5% missensePE840 FLT3 c.1831G>C p.E611Q EX14 5.8% missensePE858 PTPRT c.3214C>T p.H1072Y EX24 21.7% missensePE840 TYR c.861T[3>1] p.L288Mfs*12 EX2 2.5% frameshiftPE801 TP53 c.730G>A p.G244S EX7 35.2% missensePE938 EGFR c.2369C>T p.T790M EX20 0.6% missensePE913 NSD1 c.2122C>A p.H708N EX5 1.1% missensePE252 MTOR c.3365T>G p.F1122C EX22 1.1% missensePE270 TP53 c.527G>A p.C176Y EX5 19.0% missensePE264 MYD88 c.794T>C p.L265P EX5 87.2% missensePE351 EGFR c.2369C>T p.T790M EX20 13.9% missensePE344 SMO c.354C>A p.C118* EX2 23.2% nonsensePE386 TP53 c.1045G>T p.E349* EX10 6.3% nonsensePE364 HSP90AA1 c.1423G>C p.E475Q EX7 1.1% missensePE420 EGFR c.2573T>G p.L858R EX21 1.0% missensePE419 DNMT3A c.2206C>T p.R736C EX19 0.5% missensePE503 EGFR c.2390G>C p.C797S EX20 1.4% missensePE517 TERT c.-58-u6G>A . . 3.0% promoterPE584 DNMT3A c.1138G>C p.A380P EX10 1.0% missensePE358 EGFR c.2573T>G p.L858R EX21 28.0% missensePE582 RBM10 c.2488C>T p.R830* EX20 77.6% nonsensePE552 MLL2 c.11320C>T p.Q3774* EX39 43.6% nonsensePE654 LRP1B c.3599C>A p.P1200H EX23 6.7% missensePE644 TP53 c.390CAA[2>1] p.N131del EX5 31.8% cds-delPE689 FGF23 c.593G>C p.R198P EX3E 4.8% missensePE757 TP53 c.589_593delGTGGAp.V197Rfs*10 EX6 51.3% frameshiftPE787 CUL3 c.658G>C p.E220Q EX6 1.6% missensePE725 ARID1A c.2183_2189delCACCCAGp.P728Lfs*12 EX6 35.1% frameshiftPE768 FLT3 c.1294A>G p.T432A EX10 4.4% missensePE592 AKT3 c.329A>G p.Q110R EX4 6.4% missensePE919 B2M c.7C>T p.R3C EX1 2.4% missensePE262 RB1 c.1303G>T p.G435* EX13 1.4% nonsensePE424 GATA2 c.1010G>A p.R337Q EX4 1.6% missensePE436 RB1 c.2164A[4>2] p.K722Nfs*28 EX21 5.4% frameshiftPE472 SDHAF2 c.392T>A p.I131K EX4E 2.6% missensePE467 SMAD4 c.1309-2A>T . IVS10 2.9% splice-3

PE383 FANCD2 c.3236C>G p.S1079C EX33 26.9% missensePE441 EGFR c.2237_2255delAATTAAGAGAAGCAACATCinsTp.E746_S752delinsVEX19 13.9% cds-indelPE439 TGFBR1 c.423_428dupCATGTTp.M142_L143insFMEX3 16.1% cds-insPE397 ERBB2 c.973G>C p.E325Q EX8 1.9% missensePES121 BRAF c.958G>A p.A320T EX7 0.4% missensePE528 RBM10 c.2863-2A>G . IVS23 11.0% splice-3PE531 KDM6A c.598T>A p.C200S EX7 10.3% missensePE581 RET c.2625G>T p.L875F EX15 56.0% missensePE587 MET c.3029-1289G>A . IVS14 2.6% intronPE572 TP53 c.839G>A p.R280K EX8 62.8% missensePE598 EGFR c.2236_2250delGAATTAAGAGAAGCAp.E746_A750del EX19 1.7% cds-delPE737 CTNNA1 c.46A>G p.K16E EX2 1.1% missensePE690 WHSC1L1 c.3950A>G p.K1317R EX23 21.8% missensePE622 TP53 c.461G>T p.G154V EX5 1.1% missensePE687 LRP1B c.11009-2A>T . IVS71 2.1% splice-3PE758 TP53 c.589_593delGTGGAp.V197Rfs*10 EX6 16.6% frameshiftPE755 EGFR c.2240_2254delTAAGAGAAGCAACATp.L747_T751del EX19 2.9% cds-delPE858 DCC c.1735G>A p.D579N EX11 10.4% missensePE801 TBX3 c.104G>T p.G35V EX1 1.0% missensePE938 EPHB1 c.121G>A p.G41R EX2 10.9% missensePE917 TP53 c.620delA p.D207Vfs*40 EX6 18.7% frameshiftPE250 LRP1B c.4039_4044delGGAAACinsTp.G1347Yfs*57 EX25 2.0% frameshiftPE297 EGFR c.2236_2250delGAATTAAGAGAAGCAp.E746_A750del EX19 19.0% cds-delPE243 DYSF c.6188G>A p.R2063H EX55 2.9% missensePE207 ERBB2 c.262C>A p.H88N EX3 20.5% missensePE382 HGF c.2104C>T p.R702C EX18 3.7% missensePE393 EGFR c.2236_2250delGAATTAAGAGAAGCAp.E746_A750del EX19 1.9% cds-delPE394 TP53 c.743G>A p.R248Q EX7 21.6% missensePE527 TP53 c.646G>A p.V216M EX6 40.1% missensePE318 NOTCH1 c.6799G>C p.E2267Q EX34E 18.6% missensePE593 EPHA2 c.2671G>A p.V891M EX16 10.2% missensePE654 MLL2 c.8971C>T p.P2991S EX34 26.0% missensePE713 NTRK3 c.2368G>T p.E790* EX20E 1.8% nonsensePE535 EGFR c.2235_2249delGGAATTAAGAGAAGCp.E746_A750del EX19 5.4% cds-delPE668 POLE c.101G>A p.R34H EX2 28.2% missensePE709 MLL3 c.10231C>G p.Q3411E EX43 6.2% missensePE795 NOTCH3 c.6575G>A p.G2192E EX33E 11.5% missensePE564 ARID1B c.1106G>C p.S369T EX1 7.9% missensePE685 EGFR c.2369C>T p.T790M EX20 0.6% missensePE800 LRP1B c.12459A>T p.L4153F EX81 6.8% missensePE863 FGFR4 c.2170G>C p.E724Q EX17 1.0% missensePES121 APC c.7421C>A p.P2474H EX17 0.5% missensePES121 APC c.3580T>A p.S1194T EX17 0.5% missensePES121 AQP12A c.83T>G p.L28R EX1 0.5% missensePES121 BRCA1 c.3694G>T p.G1232C EX10 0.5% missensePES121 CCND1 c.839A>T p.E280V EX5 0.1% missensePES121 CDH23 c.6275C>A p.T2092K EX48 1.2% missensePES121 CDS1 c.288G>A p.M96I EX3 11.1% missensePES121 CHEK2 c.298C>T p.Q100* EX2 12.2% nonsensePES121 CNTN5 c.446G>A p.R149H EX5 4.6% missensePES121 CTNNB1 c.95A>G p.D32G EX3 3.7% missensePES121 CTNNB1 c.1528A>T p.T510S EX10 0.5% missensePES121 DDR2 c.1340T>A p.L447Q EX13 0.6% missensePES121 EGFR c.2369C>T p.T790M EX20 2.9% missense

PES121 EGFR c.2236_2250delGAATTAAGAGAAGCAp.E746_A750del EX19 25% cds-indelPES121 EGFR c.2369C>T p.T790M EX20 0.2% missensePES121 EGFR c.2235_2249delGGAATTAAGAGAAGCp.E746_A750del EX19 14.1% cds-indelPES121 EGFR c.2369C>T p.T790M EX20 14.9% missensePES121 ERBB2 c.1886T>A p.V629D EX20 6.2% missensePES130 FCGR2B c.820A>C p.N274H EX7 0.5% missensePES121 FLT4 c.3986G>T p.G1329V EX30 0.4% missensePES121 HGF c.1031T>C p.F344S EX8 0.5% missensePES121 JAK2 c.1849G>T p.V617F EX14 0.5% missensePES121 JAK3 c.2125T>A p.W709R EX16 0.2% missensePES121 MET c.1039G>A p.A347T EX2 12.2% missensePES121 MICALL1 c.195G>T p.L65F EX2 0.8% missensePES130 MPL c.1771T>G p.Y591D EX12 0.3% missensePES121 NTRK1 c.1624G>A p.V542I EX14 6.6% missensePES121 NTRK3 c.2296T>A p.Y766N EX19 0.2% missensePES121 NTRK3 c.2371C>T p.R791W EX20 7.2% missensePES121 NTRK3 c.905A>T p.Y302F EX9 0.3% missensePES121 OR2T4 c.55A>T p.M19L EX1 0.9% missensePES121 PSMB5 c.128C>T p.A43V EX1 12.9% missensePES121 PTPN11 c.1492C>T p.R498W EX13 0.6% missensePES121 SETD2 c.2655_2673delTCTTCCACCAGGAATAAAGp.S885Rfs*5 EX3 2.9% frameshiftPES121 SETD2 c.7093C>T p.Q2365* EX16 6.9% nonsensePES121 SPTA1 c.634G>T p.G212W EX5 2.5% missensePES121 TP53 c.842A>G p.D281G EX8 12.5% missensePES121 TP53 c.568C>T p.P190S EX6 3% missensePES121 TP53 c.818G>A p.R273H EX8 3.8% missensePES121 TP53 c.613T>C p.Y205H EX6 31.4% missensePES121 TP53 c.451C>T p.P151S EX5 20% missensePES130 EGFR c.2573T>G p.L858R EX21 16.9% missensePES130 JAK1 c.1979A>G p.D660G EX14 1.8% missensePES130 PDGFRB c.2614C>G p.P872A EX19 1.0% missensePES130 MAP3K1 c.3894G>A p.M1298I EX16 0.9% missensePES130 MLL4 c.1096G>T p.E366* EX3 0.6% nonsensePES130 RHOA c.454G>T p.G152C EX5 0.5% missensePES130 CDKN2A c.150+1_150+2insG. IVS1 0.5% splice-5PES130 EGFR c.2573T>G p.L858R EX21 49.3% missensePES130 JAK1 c.1979A>G p.D660G EX14 10.1% missensePES130 TP53 c.731G_732CdelinsTp.G244Vfs*3 EX7 7.4% frameshiftPES130 CDKN2A c.150+1_150+2insG. IVS1 6.6% splice-5PES130 PDGFRB c.2614C>G p.P872A EX19 6.0% missensePES130 MLL4 c.1096G>T p.E366* EX3 5.9% nonsensePES130 MAP3K1 c.3894G>A p.M1298I EX16 5.0% missensePES130 XPO1 c.2977C>G p.Q993E EX24 1.5% missensePES130 MTOR c.6073G>A p.E2025K EX44 0.8% missensePES131 EGFR c.2236_2250delGAATTAAGAGAAGCAp.E746_A750del EX19 0.4% cds-delPES121 SETD1B c.125A>G p.K42R 1% missensePES121 BRD3 c.146C>T p.T49M 0.7% missensePES121 ACIN1 c.822GGA[6>5] p.E270[12>11] 0.6% cds-delPES121 EZH2 c.1786G>A p.A596T 0.3% missensePES121 KRAS c.35G>A p.G12D EX2 15.3% missensePES121 TP53 c.503A>C p.H168P EX5 5.5% missensePES121 KRAS c.35G>A p.G12D EX2 62.7% missensePES121 TP53 c.503A>C p.H168P EX5 49.8% missensePES121 EGFR c.2573T>G p.L858R EX21 2.9% missense

PES121 EGFR c.2573T>G p.L858R EX21 43.8% missensePES121 MAPK3 c.642G>C p.E214D EX4 2.5% missensePES121 STAT3 c.1265delG p.G422Afs*9 EX14 0.2% frameshiftPES121 KRAS c.35G>A p.G12D EX2 59.5% missensePES121 TP53 c.844C>T p.R282W EX8 63.9% missensePES121 KRAS c.35G>A p.G12D EX2 54.2% missensePES121 TP53 c.844C>T p.R282W EX8 61.6% missensePES121 CDK4 c.602G>T p.G201V EX5 0.5% missensePES121 IRS2 c.3515A>T p.H1172L EX1 0.4% missensePES121 NF1 c.7501A>T p.M2501L EX50 0.4% missensePES121 MTOR c.4180G>T p.A1394S EX28 0.6% missensePES121 NF1 c.2431A>T p.T811S EX21 0.5% missensePES130 EGFR c.2236_2250delGAATTAAGAGAAGCAp.E746_A750del EX19 21.6% cds-delPES130 TP53 c.842A>G p.D281G EX8 30.2% missensePES130 EGFR c.2236_2250delGAATTAAGAGAAGCAp.E746_A750del EX19 34.2% cds-delPES130 JAK2 c.1849G>T p.V617F EX14 1.2% missensePES130 TP53 c.842A>G p.D281G EX8 19.7% missensePES121 BRAF c.958G>A p.A320T EX7 0.4% missensePES121 CSF1R c.1024T>C p.F342L EX7 0.3% missensePES121 DNMT3A c.2329C>A p.P777T EX20 0.9% missensePES121 EGFR c.2156G>C p.G719A EX18 13.2% missensePES121 EGFR c.2156G>C p.G719A EX18 16.6% missensePES121 EGFR c.2303G>T p.S768I EX20 9.6% missensePES121 EGFR c.2303G>T p.S768I EX20 15.3% missensePES121 KIF5B c.515T>G p.F172C EX7 0.5% missensePES121 MLL2 c.5713C>G p.Q1905E EX26 3.6% missensePES121 MLL2 c.5713C>G p.Q1905E EX26 3.7% missensePES121 PMS1 c.1948G>T p.G650C EX10 0.5% missensePES121 PTPRD c.757G>A p.V253I EX20 0.3% missensePES121 TP53 c.464C>A p.T155N EX5 1.2% missensePES121 TP53 c.517G>T p.V173L EX5 4.4% missensePES121 TP53 c.517G>T p.V173L EX5 3.2% missensePES121 TYR c.1088A>T p.H363L EX3 0.7% missense