Enhance your screening ability and advance your research by using the gold standard reagent for the study of histone and DNA methylation – Adenosyl- L-Methionine, S-[methyl-3H]; (3H-SAM). This reagent enables both quantitative

kinetic measure of substrate methylation rates and the measure of methyltransferases with low activity. By using 3H-SAM, the labeled methyl group is directly incorporated without disrupting the biology of the reaction you’re studying. Save time and money – there is no need for detection antibodies or signal amplification reagents.

www.perkinelmer.com/3H-SAM

3 H-SAM Methylation Assay

3H-SAM FOR EPIGENETICSRESEARCH

Gold standard detection

• High sensitivity to detect low levels of enzyme activity

• Direct methylation measurement

• No antibodies

• Reliable screening method for over 50 years

Target areas of study

• Cancer

• Stem cells

• Immunology

• Neurodegenerative diseases

• And many others

• SPA Beads and FlashPlates®

• Liquid scintillation analyzers

• Liquid scintillation and luminescence counters

• Gamma counters

• Radiomatic flow scintillation analyzers

• Microplates and vials

• Liquid scintillation cocktails

• Radiation safety equipment

• Cytostar-T® and scintillation microplates

For a complete listing of our global offices, visit www.perkinelmer.com/ContactUs

Copyright ©2010, PerkinElmer, Inc. All rights reserved. PerkinElmer® is a registered trademark of PerkinElmer, Inc. All other trademarks are the property of their respective owners. 009197_01 Printed in USA Jun. 2010

PerkinElmer, Inc. 940 Winter Street Waltham, MA 02451 USA P: (800) 762-4000 or (+1) 203-925-4602www.perkinelmer.com

Complete Radiometric SolutionsFrom quality microplates, vials, scintillation cocktails, scintillation proximity assay (SPA) technologies and instruments. PerkinElmer offers complementary products for your radioactive assays.

Tri-Carb®

MicroBeta2TM

ORdERING INFORMATIONTo choose the right product for your methylation assays, PerkinElmer offers several Adenosyl-L-Methionine, S-[methyl-3H] products varying in solvent, specific activity, concentration and package size.

Product Specific Activity Concentration Size Part Number

Adenosyl-L-Methionine, S-[methyl-3H] 5-15 Ci (185-555 GBq)/mmol 0.55 mCi (20.35 MBq)/mL 50 uCi (1.85 MBq) NET155050UC

5-15 Ci (185-555 GBq)/mmol 0.55 mCi (20.35 MBq)/mL 250 uCi (9.25 MBq) NET155250UC

5-15 Ci (185-555 GBq)/mmol 0.55 mCi (20.35 MBq)/mL 1.0 mCi (37 MBq) NET155001MC

Packaged in 10 mM sulfuric acid: ethanol (9:1). Shipped in dry ice.

Adenosyl-L-Methionine, S-[methyl-3H] 55-85 Ci (2.03-3.15 TBq)/mmol 0.55 mCi (20.35 MBq)/mL 250 uCi (9.25 MBq) NET155H250UC

55-85 Ci (2.03-3.15 TBq)/mmol 0.55 mCi (20.35 MBq)/mL 1.0 mCi (37 MBq) NET155H001MC

Packaged in 10 mM sulfuric acid: ethanol (9:1) under argon. Shipped in dry ice.

Adenosyl-L-Methionine, S-[methyl-3H] 12-18 Ci (444-666 GBq)/mmol 1.0 mCi (37 MBq)/mL 250 uCi (9.25 MBq) NET155V250UC

12-18 Ci (444-666 GBq)/mmol 1.0 mCi (37 MBq)/mL 1.0 mCi (37 MBq) NET155V001MC

Packaged in hydrochloric acid, pH 2.0-2.5, under argon. Shipped in dry ice.

3H-SAM is part of PerkinElmer’s comprehensive solution for understanding cellular signaling and morphology in context. Only PerkinElmer provides multidirectional biochemical and cellular approaches, which enable researchers to locate, detect and quantitate complex cellular events in the areas of cancer and epigenetic research.