salt and water homeostasis 1. human 2. toad bladder 3. water pore (red cell) module c 10 june 2013...
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Salt and Water Homeostasis1. Human2. Toad Bladder3. Water Pore (red cell)
Module C10 June 2013Elyse Sadow, Pouya Jamshidi, Liza LichtenfeldKeais Pope, Samantha Harrington
Experiment
• Purpose: To determine the Kidney’s response to Water Load
1) 9 subjects. Two x 3hr Urine collections.2) Intervention: 5 Subjects Water-Loaded (4% TBW)3) Control: 4 subjects, 1 cup of water4) Measured Urine Volume, Osm, Chloride5) Calculated Excretion of Free Water, Salt, and Urea
44mL/kg=~4% tbw
Control before vs. after
Control before vs. after
InterventionBefore vs. After
InterventionBefore vs. after
Excretion equations
• Free Water Clearance per Hour=• [UVol-(UVol*UOsm/300)]/3hr
• UVolactual*UOsmactual=UVol300 *UOsm300
• Salt Excretion=(2*Cl)*UVol
• Urea=UOsm-(2*Cl)• Urea Excretion=Urea* UVol
•
-0.15
-0.1
-0.05
0
0.05
0.1
0.15
0.2
0.25
0.3
0.35
1 2
Free water excretion
Salt excretion
0
20
40
60
80
100
120
140
160
180
1 2
Urea excretion
-100
-50
0
50
100
150
1 2
Experimental Set-Up
Time (min)
0 20 40 60 80 100 120 140 1600
0.2
0.4
0.6
0.8
1
1.2
Sample 1Sample 2Sample 3Sample 4Sample 5Sample 6Sample 7%
wei
ght c
hang
eADH
Experimental Data
Data Average
0 20 40 60 80 100 120 140 1600
0.2
0.4
0.6
0.8
1
Time (min)
% w
eigh
t cha
nge
ADH
ADH in Action
ATP
cAMP
AC AVP
BasolateralApical
V2 receptor
ADH in Action
ATP
cAMP
AC AVP
BasolateralApical
AQP-2
•Examining Water Pores in Red Blood Cells
Before mixing
After mixing
During mixing
H2O H2O
Schematic of Stopped- flow Fluorimeter
Isotonic vs Hypertonic
Inhibition of Water Transport
Glycerol Flow
Question?