safety washing hands frequently airflow no smoking or eating in lab wearing protective clothing and...
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Safety
• Washing hands frequently • Airflow• No smoking or eating in lab• Wearing protective clothing and use of protective equipment.
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Specimen Information
• Laboratory serial number• Date received• Patient’s name, sex, age, & address• Name of Health Institution• Reason for exam
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Standard reagents:
• Carbol Fuchsin• Acid/Alcohol or Sulfuric Acid• Methylene Blue
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• 100 fields!!
• Examining fewer than 100 fields can cause a false negative report.
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Discard of Specimens
• Burning
• Boiling
• Autoclaving
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WHO and IUATLD
• Negative Report: “Negative for acid-fast bacilli where no organisms have been observed in 100 fields.
• Positive Report: “Positive for acid-fast bacilli : Provide AFB quantitation.
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Semi-quantitative reportingZiehl Neelsen
• No AFB are found in 100 fields: “No acid-fast bacilli observed.”• 1-9 /100 fields: Report the exact figure.• 10-99 AFB /100 fields, 1+• 1-10AFB /field, 2+• Greater than 10 AFB/ field, 3+
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Information
• Evaluation of the quality of the specimen• Staining method used (Ziehl Neelsen)• Smear result• Date of exam• Signature of microscopist
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Some reasons for false positive results:
• Errors in specimen handling or recording information
• Re-use of containers or positive slides• Unfiltered fuchsin• Contaminated immersion oil• Inadequate decolorization
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Some reasons for false negative results:
• Errors in specimen handling or recording information
• Poor quality sputum• Excessive decolorization• Reading less than 100 microscopic fields
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