s2 6 ravindran - biosolids · rmituniversity©august2017 6. rmituniversity©august2017 7 0 10 20 30...
TRANSCRIPT
A modified assay for the enumeration ofascaris eggs in fresh raw sewage
VIVEK RAVINDRAN (PhD student)
Supervisors:
Prof. Andy Ball
Dr. Sarvesh Kumar Soni
PATHOGENS in sewage
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Distribution of helminth ova genera in wastewater
Source: Jiminez et al., 2007
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Ascaris – The neglected parasiteØ 1.5 billion people infected worldwideØ Enormous egg production ( 240,000 eggs/ day/ female )Ø Eggs are highly resistant and may remain viable for several years
A.Suum 7 days embryonation larvae emerging from the egg Adult worms
Fertlized egg Unfertlized egg SEM image
AIM & OBJECTIVES
ØOptimize the concentration and enumeration of helminth eggs
ØDevelop a reproducible and cost effective protocol for yielding better recovery rates of eggs from sewage
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MODIFIED BOWMAN METHOD
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Boneo PakenhamMt. Martha Somers Longwarry Koo Wee Rup
Blind Bight
Reco
very
%
Treatment Plants
Ascaris eggs recovered
WHO Method
Modified Bowman Method
Results
Conclusion
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Ø Better recovery of 42% with modified Bowman method compared to the
standard Bowman method
Ø Modified Bowman method is a simple, low cost method for routine use by
commercial laboratories and researchers
Ø Need to improve identification by developing and comparing methods of
detection
Ø Ongoing identification methods in my project:
Ø Propidium Monoazide-qPCR to detect viable Ascaris ova from wastewater
Ø RPA-SERS/Lateral strips for point-of-care diagnosis
Ø Enzyme based -Colorimetric analysis
Ø Nanozyme activity (in-situ synthesized metal nanoparticles)
ACKNOWLEDGEMENT
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ü Prof. Andy Ball, School of Sciences, Bundoora Campus RMITUniversity, Australia
ü Dr. Sarvesh Kumar Soni, School of Sciences, Bundoora Campus RMIT University, Australia
ü Dr. Aravind Surapaneni, South East Water, Australia
ü Dr. Esmaeil Shahsavari, Arturo Aburto-Medina, Basma Khallaf, colleagues and Staffs (School of Sciences, RMIT)
ü Dr. Jonathan Schmidt, South East Water, Frankston, Australia
ü Dr. Nicholas Crosbie, Melbourne Water, Australia
Methods X : http://www.sciencedirect.com/science/article/pii/S2215016117300158
Further contact: [email protected] (Vivek), [email protected], [email protected]