rose bengal test antigen for the serological diagnosis of ... · an antigen prepared from b....

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Description: How to use: An antigen prepared from B. abortus (Strain 99) with Rose Bengal dye and suspended in acid buffer (pH 3.65). Used to detect Brucella antibodies in serum - using a plate agglutination test. It detects antibodies against B. abortus, B. melitensis, and B. suis in serum samples. Ÿ Bring the RBT antigen and samples (sera) to room temperature before beginning the test. Ÿ Place 30 µl of each serum sample on the agglutination plate. Ÿ Shake antigen bottle gently before use and place 30 µl of RBT antigen next to the serum sample on agglutination plate. Mix the antigen and the serum. Ÿ Shake the plate for 5 minutes and read. No agglutination indicates negative sample. Agglutination indicates positive sample (Please note that slight agglutination is also considered as a positive result). We recommend using VLA positive control (Cat #RAB1003) for each batch of samples and for newly opened antigen bottles. 10 ml of Rose Bengal Test Antigen per bottle. Store in the dark at +2°C to +8°C. Do not freeze. V2001 Results: Validation of the test: Presentation: Storage: Product Code: Rose Bengal Test Antigen for the Serological Diagnosis of Brucellosis Lillidale Diagnostics Pig Oak Farm Holt Wimborne Dorset BH21 7DG United Kingdom Tel: +44 (0) 1202 848456 Fax: +44 (0) 1202 848570 Email: [email protected] Website: www.lillidale.co.uk Lillidale Diagnostics is a trading name of Lillidale Ltd.

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Page 1: Rose Bengal Test Antigen for the Serological Diagnosis of ... · An antigen prepared from B. abortus (Strain 99) with Rose Bengal dye and suspended in acid buffer (pH 3.65). Used

Description:

How to use:

An antigen prepared from B. abortus (Strain 99) with Rose Bengal dye and suspended in acid buffer (pH 3.65). Used to detect Brucella antibodies in serum - using a plate agglutination test. It detects antibodies against B. abortus, B. melitensis, and B. suis in serum samples.

Ÿ Bring the RBT antigen and samples (sera) to room temperature before beginning the test.Ÿ Place 30 µl of each serum sample on the agglutination plate.Ÿ Shake antigen bottle gently before use and place 30 µl of RBT antigen next to the serum sample on agglutination

plate. Mix the antigen and the serum.Ÿ Shake the plate for 5 minutes and read.

No agglutination indicates negative sample.Agglutination indicates positive sample (Please note that slight agglutination is also considered as a positive result).

We recommend using VLA positive control (Cat #RAB1003) for each batch of samples and for newly opened antigen bottles.

10 ml of Rose Bengal Test Antigen per bottle.

Store in the dark at +2°C to +8°C. Do not freeze.

V2001

Results:

Validation of the test:

Presentation:

Storage:

Product Code:

Rose Bengal Test Antigenfor the Serological Diagnosis of Brucellosis

Lillidale DiagnosticsPig Oak FarmHoltWimborneDorsetBH21 7DGUnited Kingdom

Tel: +44 (0) 1202 848456Fax: +44 (0) 1202 848570Email: [email protected]: www.lillidale.co.uk

Lillidale Diagnostics is a trading name of Lillidale Ltd.