REVIEW & STUDY DAYFriday, May 9, 2014

OBJECTIVE FOCI(VOLUNTEER TO READ)

Describe how radioactive probes are used in DNA fingerprinting

Explain how DNA fingerprinting is used to determine if specimens come from related or unrelated individuals

Explain how to use DNA fingerprinting to identify DNA from a parent, child, or relative of another person

RULES(TWO VOLUNTEERS TO READ)

Questions will rotate around the room (counter-clockwise… Asia G Cameron DeAsia Asiah W Ca’Juan Cortez Asia G again

Teams have 1 minute (60 seconds) to answer the question

If a team answers a questions incorrectly, it goes to the next team

If a team speaks out while it is another team’s turn, they lose their turn

If a team answers a question correctly, they have a chance to try the next

No notes, articles, or electronics allowed. Only blank paper and writing utensils allowed to take notes on things you need to study

If teams cheat (using notes, electronics, etc.), they lose three points the first time and lose the game entirely the second time

QUESTIONS?

DECIDING WHO GOES FIRST

On a half-sheet of paper, write your best answer to this question:

Why do forensic scientists call this type of evidence DNA fingerprinting?

You have one minute ONLY to write your answer and hand it to Mr. Valasek, so he can pick the best answer to go first.

Ties broken by best out of three of rock-paper-scissors

QUESTION 1

ElectrophoresisExpulsionAmplificationRestriction Fragments

Pick the step that does not belong in the following list of how to DNA fingerprint

QUESTION 2

ElectrophoresisExtractionAmplificationRestriction Fragments

Put the steps in the following list of how to DNA fingerprint in their correct order

QUESTION 2

ExtractionRestriction FragmentsAmplificationElectrophoresis

Pick the step in the following list that means making enough DNA for DNA fingerprinting

QUESTION 3

ExtractionRestriction FragmentsAmplificationElectrophoresis

Pick the step in the following list that means isolating the DNA from the rest of the biological material

QUESTION 4

ExtractionRestriction FragmentsAmplificationElectrophoresis

Describe why electrophoresis would be ineffective without restriction fragments

Restriction fragments are the “sliced-up” pieces of DNA that show the VNTRs & STRs, so that DNA can be used as individualized evidence… Electrophoresis would simply show one big dot if no restriction fragments were cut

QUESTION 5 Agarose Gel

Separating DNA fragments

For the following question, explain how the next two ideas are related Electrophoresis separates the DNA fragments in agarose

gel, because of its internal matrix (or criss-crossing fiberous structure)

QUESTION 6

Negatively-charged drop well

Positively-charged electrode

For the following question, explain how the next two ideas are related Within the agarose gel, the DNA fragments are dropped into

the negatively-charged well and migrate toward the positively-charged electrode when the electric current begins

QUESTION 7 Probes

Digital ImagingGenes

If geneticists decide not to amplify their sample, what must they use instead to see the results?

QUESTION 8

20,000 base pairs10,000 base pairs5,000 base pairs15,000 base pairs

Place the following fragments in order as they would appear in gel electrophoresis

QUESTION 9 Protein and a gene

Base pairs and a radioactive compound

Alleles and a chromosome

DNA Probes are composed of two parts:

QUESTION 10

TTCGAAT

A) TTCGAATB) AAGCUUAC) AAGCTTAD) TAAGCTT

The following probe would highlight which DNA fragment:

QUESTION 11

agcttgcaacgtaatcccgtaactattattattattattattatgcgaaggaggagggaggcgtatggagggtttcttaacgtattatct

Find the portion of this sequence a geneticist would want to highlight with a DNA probe

QUESTION 12

Digital imageAutoradiographX-rayDouble-helix

When a geneticist decides to develop a film image of the electrophoresis, they call this:

QUESTION 13 Greater, lesser

Lesser, greaterGreater, greater

Fill in the blanks.“The _____ the number of probes used on a DNA fingerprint, the _____ the accuracy of the DNA fingerprint”

QUESTION 14 B. How many do

they receive from each parent?

2, 1, or ½

A. Children receive how many genes of each trait?

2, 1, or 4

QUESTION 15

Crime-scene DNA matching with suspect’s DNA

Discovery of dinosaur remains

Paternity and maternity determination

Identify family members and relatives

Suspect elimination

Finding good candidates for space flight

Freeing those who have been falsely accused

Identification of human remains

Find all the wrong uses of DNA fingerprinting from this list

WHO’S WHOSE DADDY?Follow these steps to find the father of each child.

WHO’S THE CRIMINAL?Follow the steps from Wednesday (from memory) to find the criminal out of the three suspects