review game genetic engineering. best arnold character 1. 2. 3. 4
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Review Game
GENETIC ENGINEERING
Best Arnold character
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1. Terminator2. Predator3. Mr. Freeze4. Kindergarten Cop
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2.
3.
4.
DNA that is from two or more organisms is called…
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1. Combinant DNA2. Remade DNA3. Recombinant DNA4. Multiple DNA
Fastest Responders (in seconds)
4.91 KAYLA COCKRELL
5.69 ETHAN WHITE
6.09 NORA WAARANIEMI
7 EMILY TURNQUIST
7.6 MICHAEL GIBBS
Today’s Topics
Genetic Engineering
Stem CellsGel Electrophoresis
GMOs and Cloning
Final Results
Genetic Engineering
Which of these is not an example of a vector used in genetic engineering?
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1. Plasmid2. Yeast3. Virus4. Bacteria
What is used to cut DNA?
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1. Restriction Enzymes2. DNA Ligase3. Scissors4. Recombinant Enzymes
If I wanted to produce human insulin using a transformed bacteria, what would I need to do first?
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1. Recombine the DNA with a vector
2. Cut out the gene of interest
3. Isolate the gene of interest
4. Insert the gene into the bacteria
I have now made my Recombinant DNA. What must take place before I can clone the gene?
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1. Transform the bacteria2. Select and screen3. Gene cloning4. Use DNA Ligase
DNA fragments cut with EcoRI can be combined with DNA cut with
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1. BamHI2. HindIII3. EcoRI4. All of the above5. 1 & 26. 2 & 3
Restriction fragments cut with a restriction enzyme have a single- stranded 4 base tail of AATT. What is this called?
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1. Blunt end2. Sticky end3. Restriction tail4. Ligase
How does the gene get cloned once it has been taken in by the bacteria?
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1. Using PCR2. Do more
transformations3. Bacterial reproduction4. Gel electrophoresis
Once I have selected and screened the bacteria, I can now …
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1. Extract the gene2. Extract the protein3. Give the bacteria to an
ill person4. All of these5. 1 & 26. 1 & 3
Fastest Responders (in seconds)
11.89 MICHAEL GIBBS
15.57 KATHERINE SPURLOCK
18.42 THOMAS NETTLETON
32.7 SAMUEL KAUTZKY
Team Scores
63.33 Terminator
53.75 Kindergarten Cop
51.1 Mr. Freeze
50 Predator
GMOs and Cloning
FASTEST FINGER
Which is easier to clone?
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1. Animals2. Plants3. Humans
Which of these is not a reason for making crops GMOs?
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1. Better Taste2. Drought Resistance3. Pest Resistant4. All of the above5. Only 2 and 3
If I wanted to clone a sheep 1, using an egg from sheep 2, and having sheep 3 deliver the baby, where do I get the DNA from?
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1. Sheep 12. Sheep 23. Sheep 3
The method of cloning described in the last slide is called?
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1. Nuclear replacement2. Somatic cell nuclear
transplantation3. Artificial twinning4. Egg cell fertilization
Which type of cell can I get the DNA from?
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1. Heart cell2. Skin cell3. Liver cell4. Muscle cell5. None of these6. All of these
Fastest Responders (in seconds)
10.77 NORA WAARANIEMI
11.11 BRANDON LINGEN
12.1 KATHERINE SPURLOCK
12.79 KYLIE JOHNSON
19.38 LIESE PETERSON
Team Scores
88.2 Terminator
78.65 Mr. Freeze
77.52 Kindergarten Cop
75.77 Predator
Gel Electrophoresis
FASTEST FINGER
Gel Electrophoresis does what?
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1. DNA Fingerprinting2. Paternity testing3. Separates molecules
by size4. Uses electricity
Since everyone on the planet has different DNA, identical twins excluded,
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1. No one has the same DNA fingerprint
2. Most people have a different DNA fingerprint
3. You are less likely to have the same DNA fingerprint as another
In RFLP analysis, with what do we mix our DNA samples before running them through a gel
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1. DNA ligase2. plasmids3. Restriction enzymes4. DNA polymerase
Who is the baby’s father?
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1. Bob 2. Larry3. Neither
Which direction would DNA move while being separated in gel electrophoresis?
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1. Towards the positive2. Towards the negative3. Both directions
depending on the person
Which DNA Fragment is shorter?
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1. A2. B
Who matches the blood stain?
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1. Bob2. Sue3. John4. Lisa
PCRs main function is to
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1. Exponentially Amplify DNA
2. Make a few copies of DNA
3. Catch criminals4. Extract DNA
Fastest Responders (in seconds)
3.13 MICHAEL GIBBS
5.69 ERIN LOUWAGIE
6.01 KEVIN STOWE
8.33 NORA WAARANIEMI
8.58 CALEB TROUTH
Team Scores
117.94 Predator
117.78 Terminator
110.98 Mr. Freeze
102.19 Kindergarten Cop
Stem Cells
Which of these can differentiate into the widest variety of cells?
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1. Totipotent2. Multipotent3. Pluripotent
Embryonic stem cells are
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1. Totipotent2. Pluripotent3. Multipotent
Where do most adult stem cells used in medicine come from?
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1. Kidneys2. Skin3. Blood4. Bone marrow
Adult stem cells are
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1. Totipotent2. Pluripotent3. Multipotent
Which type of stem cell is created from a differentiated somatic cell?
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1. Adult stem cells2. Embryonic stem cells3. Induced pluripotent
stem cells
Fastest Responders (in seconds)
4.87 MICHAEL GIBBS
Team Scores
142.76 Mr. Freeze
137.94 Predator
137.78 Terminator
132.19 Kindergarten Cop
Team MVP
Points Team Participant
179.91 Mr. Freeze MICHAEL GIBBS
147.07 Predator ERIN LOUWAGIE
151.84 Terminator TIFFANY LO
163.27 Kindergarten Cop EMILY TURNQUIST
Participant Scores
179.91 MICHAEL GIBBS 129.4 TRISHA MORRISON
165.66 BRANDON LINGEN 126.43 TAYLOR HAEFELE
163.27 EMILY TURNQUIST 120.2 RISHA SINGH
151.84 TIFFANY LO 118.49 KAYLA COCKRELL
150.67 CALEB TROUTH 118.43 KYLIE JOHNSON
147.07 ERIN LOUWAGIE 115.6 PETER KILIAN
141.37 KATHERINE SPURLOCK
112.91 PETER FOMIN
135.43 LIESE PETERSON 112.38 KEVIN STOWE
132.76 NORA WAARANIEMI 103.77 THOMAS NETTLETON
132.11 ETHAN WHITE 101.31 LYDIA BOIKE