reveal what matters · the faims pro interface provides orthogonal precursor ion selectivity based...
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Reveal what matterswith effortless selectivity
Thermo Scientific FAIMS Pro interface
Effortless selectivity accelerates your proteomics workflowThe growing complexities of qualitative and quantitative analyses in proteomics demand faster
sample preparation, selective and sensitive analytical techniques, and efficient software solutions.
With industry-leading technical depth in mass spectrometry innovation, Thermo Fisher Scientific
enables broader and deeper analysis into the proteome than ever before.
The Thermo Scientific™ FAIMS Pro™ interface is a next-generation, differential ion mobility device that
seamlessly works with multiple Thermo Scientific™ next-generation mass spectrometers to enhance
selectivity and enable identification and quantitation of more proteins while reducing time-consuming
sample preparation. This results in increased productivity and data quality for every proteomics
user—from discovery of disease biomarkers to identification of new therapeutic targets.
The FAIMS Pro interface seamlessly connects with Thermo Scientific™ LC and MS systems such as the Thermo Scientific™ EASY-nLC™ 1200 system and the Orbitrap Exploris™ 480 mass spectrometer, to provide
selective identification of more proteins and unique peptides, increasing coverage
without extra work.
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State-of-the-art interface enhances performance and usability
With advanced hardware, the FAIMS Pro interface not only enhances experimental performance and data quality, a unique design also makes it easy to set up, use, and maintain.
• Optimized cylindrical geometry substantially increases ion transmission to the mass spectrometer with short residence times, allowing multiple compensation voltage (CV) settings per data acquisition method
• Ideally operates with typical nanoflow (100 to 1000 nL/min) chromatography separations or direct infusion experiments
• Assembly and disassembly are fast, tool-free, and do not require breaking vacuum
• Assembly, including mounting to the instrument, takes less than two minutes and is one way with perfect alignment and no fine-tuning
Flexible to fit your work
Effortlessly fits into existing workflows, maximizing sample profiling across wide dynamic loading amounts, gradient lengths, and with low-flow chromatography, to increase the productivity of proteomics experiments.
Easy to install, use, and maintain
Tool-free, one-way assembly requiring no fine-tuning nor breaking of vacuum, and predefined workflows for data acquisition and processing, ensure ultimate usability and high data quality for all users.
Increases depth of analysis without extra work
Minimizes the time, expense, and variability of offline LC fractionation by carrying out online gas phase fractionation prior to ion introduction into the mass spectrometer.
Conserves sample
Increases selectivity and sensitivity to maximize proteome coverage while conserving sample.
High voltagewaveform lead
PEEK bushings for electrode spacing
Entrance plate
Outer electrode
Inner electrode Electrode heater
O-ring for vacuum seal
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Precursor ions formed at the Thermo Scientific™ EASY-Spray™ Source emitter and introduced into the FAIMS Pro interface electrodes are selectively transmitted through the interface to the ion transfer tube and into the mass spectrometer based on the compensation voltage (CV) setting, while all other ions are filtered out, providing orthogonal selectivity and increased sensitivity.
As a result, you easily reveal what’s important
in your samples.
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Proteome analysis using the FAIMS Pro interface increases proteome coverage for both low and high sample loading amounts. Comparative analysis of the number of proteins and peptides confidently identified in an analysis of a tryptic digest of HeLa cell lysate under identical chromatographic conditions. While the proteome coverage measured using the standard DDA method at low and high sample loading amounts is impressive, simply incorporating the FAIMS Pro interface substantially increased protein and peptide coverage with almost 8,000 proteins confidently measured in the 140-minute method.
Incorporation of the FAIMS Pro interface into an existing DDA experimental workflow increases proteome profiling efficiency. Compared to using a standard DDA method, the FAIMS Pro interface method provided similar proteome coverage while using substantially less sample and shorter chromatographic gradients, addressing the two primary concerns for translational proteomics.
42,11653,95852,417
69,801
100 ng 1000 ng
More Unique Peptides (1% FDR)
Increase Proteome Profile Efficiency
Control FAIMS
+29%
+24%
5,699 6,6176,695
7,810
100 ng 1000 ng
More Protein Groups
+18%+17%
50,263
6,335
51,904
6,542
Unique peptides (1% FDR) Protein groups
1000 ng HeLa – 120 min gradient without FAIMS
200 ng HeLa – 90 min gradient with FAIMS
5× less sample25% shorter run
42,11653,95852,417
69,801
100 ng 1000 ng
More Unique Peptides (1% FDR)
Increase Proteome Profile Efficiency
Control FAIMS
+29%
+24%
5,699 6,6176,695
7,810
100 ng 1000 ng
More Protein Groups
+18%+17%
50,263
6,335
51,904
6,542
Unique peptides (1% FDR) Protein groups
1000 ng HeLa – 120 min gradient without FAIMS
200 ng HeLa – 90 min gradient with FAIMS
5× less sample25% shorter run
Orthogonal selectivity adds efficiency to proteomics workflowsThe FAIMS Pro interface integrates directly into existing workflows to enhance selectivity for proteome profiling, multiplexed protein quantitation, and top down protein characterization. Differential ion mobility reduces the complexity of precursor ions accumulated and analyzed per CV setting, increasing proteome coverage, decreasing interference, and improving quantitative confidence. In most cases, the FAIMS Pro interface provides the same coverage using less sample and in less time.
Enhanced selectivity, unmatched performance
The FAIMS Pro interface provides orthogonal precursor ion selectivity based on differential gas phase mobility. The CV setting determines which groups of ions are transmitted to the mass spectrometer for detection. A wide range of possible CV settings (-120 to 120 V) increases
instrument performance for proteomics experiments. Multiple CV settings may be repetitively sampled to increase the number and type of precursors detected and sequenced, resulting in greater ID rates using the same experimental parameters. Each CV setting uses
the same Data Dependent Acquisition (DDA) method to acquire high-resolution accurate-mass (HRAM) MS and a series of MS/MS spectra, maximizing proteome coverage. The increased selectivity and sensitivity enable researchers to increase proteome coverage, or to maximize efficiency and conserve sample.
“ We have been extremely impressed with the performance of the Thermo Scientific FAIMS device for shotgun proteomics. FAIMS enables considerably more sample depth per unit time and could eliminate the need for prefractionation of peptides for most applications.”
Joshua J. Coon Thomas and Margaret Pyle Chair at the Morgridge Institute for Research; Professor, Biomolecular Chemistry and Chemistry, University of Wisconsin; Director, NIH National Center for Quantitative Biology of Complex Systems. For more information about Coon’s work with FAIMS see, A. S. Hebert et al. AnalChem 2018, 90(15), pp. 9529–9537.
Higher-confidence quantification
Comparative TMT analysis evaluating the knock out MET6 protein ratios across four proteomes and 11 replicates. At top is the breakdown of the four-proteome mix. Each proteome was mixed 1:1 after preparation and labeling. Samples were analyzed using four DDA methods to evaluate selectivity and relative quantitation accuracy.
Relative quantification of TMT reporter ions of proteins regulated by the MET6 gene is for each acquisition method. Only the response for the MET6 knock out samples should have measured reporter ion ratios close to zero.
The interference free index plot depicts the isobaric interferences for the targeted peptides. The greater the index, the better the quantitation shown in the middle chart. The greatest selectivity and quantitative accuracy was obtained for the SPS MS3 FAIMS Pro interface method. Use of the FAIMS Pro interface for MS/MS analysis also produced a substantial increase in quantitative accuracy. Data courtesy of D. Schweppe and S. Gygi.
Increased confidence in multiplexed protein quantificationTo address the large scope of translational proteomics experiments, multiplexed proteome analysis using Thermo Scientific™ Tandem Mass Tags™ (TMT™) isobaric tags is used to simultaneously compare protein abundances across time, perturbations, tissues, etc. The challenge is to not only detect more peptides, but to maintain relative quantitative accuracy across the different samples. Using isobaric tags, the reporter ions used for relative quantitation are generated at the MS/MS or MS3 stage. However, quantitative accuracy can be compromised by sample complexity due to co-isolation of isobaric peptides resulting in distortion of reporter ion ratios. Incorporating the FAIMS Pro interface in the workflow increases precursor selectivity, resulting in greater confidence in peptide and protein quantification.
126
met6 his4 ura2
127N127C
128N128C
129N129C
130N130C
131N131C
BY4742
TMT11plex yeast digest standard
DDA Method
SPS MS3 with FAIMS SPS MS3 without FAIMS MS/MS with FAIMS MS/MS without FAIMS
0
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Met6 Normalized ProteinSumSN
340.97
350.98
480.83
320.95
0.00
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1.00
IFI
SPS MS3
without FAIMS34
0.97
SPS MS3 with FAIMS
350.98
MS/MSwithout FAIMS
480.83
MS/MS with FAIMS
320.95
Observed in Met6 KO: Median IFI:
0
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1 2 3 4 5 6 7 8 9 10 11 1 2 3 4 5 6 7 8 9 10 11 1 2 3 4 5 6 7 8 9 10 11
126
met6 his4 ura2
127N127C
128N128C
129N129C
130N130C
131N131C
BY4742
TMT11plex yeast digest standard
DDA Method
SPS MS3 with FAIMS SPS MS3 without FAIMS MS/MS with FAIMS MS/MS without FAIMS
0
25
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Met6 Normalized ProteinSumSN
340.97
350.98
480.83
320.95
0.00
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0.50
0.75
1.00
IFI
SPS MS3
without FAIMS34
0.97
SPS MS3 with FAIMS
350.98
MS/MSwithout FAIMS
480.83
MS/MS with FAIMS
320.95
Observed in Met6 KO: Median IFI:
0
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1 2 3 4 5 6 7 8 9 10 11 1 2 3 4 5 6 7 8 9 10 11 1 2 3 4 5 6 7 8 9 10 11
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met6 his4 ura2
127N127C
128N128C
129N129C
130N130C
131N131C
BY4742
TMT11plex yeast digest standard
DDA Method
SPS MS3 with FAIMS SPS MS3 without FAIMS MS/MS with FAIMS MS/MS without FAIMS
0
25
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Met6 Normalized ProteinSumSN
340.97
350.98
480.83
320.95
0.00
0.25
0.50
0.75
1.00
IFI
SPS MS3
without FAIMS34
0.97
SPS MS3 with FAIMS
350.98
MS/MSwithout FAIMS
480.83
MS/MS with FAIMS
320.95
Observed in Met6 KO: Median IFI:
0
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0
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1 2 3 4 5 6 7 8 9 10 11 1 2 3 4 5 6 7 8 9 10 11 1 2 3 4 5 6 7 8 9 10 11
“ FAIMS is appearing with perfect timeliness. We desperately need robust and reliable separation approaches that allow access to previously undetected low abundance populations of precursor peptides. FAIMS can do that with ease. And we need techniques to remove interference in isobaric tagging experiments. FAIMS does that, too.”
Steven Gygi Professor of Cell Biology, Harvard Medical School
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Selectivity expands confidence for intact protein characterizationProtein and proteoform analysis require comprehensive and accurate molecular and sub-structural characterization to enable identification and location of cSNPs, mutations, PTMs, and sequence clippings. The mass spectral method must be able to differentiate the various proteoforms to increase the confidence in automated data processing, while maintaining efficiency to address sample throughput. Proteoforms significantly increase mass spectral complexity, requiring additional workflow selectivity to increase profiling capabilities. Adding the FAIMS Pro interface enhances intact protein characterization by reducing interferences and enhancing sensitivity, enabling more accurate proteoform detection and characterization.
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Rapid characterization of the reduced and alkylated NIST mAb standard through direct infusion analysis using the Thermo Scientific™ Orbitrap™ Eclipse™ Tribrid™ mass spectrometer. Introduction of the FAIMS Pro interface prior to HRAM MSn analysis facilitated gas-phase separation based on the CV setting. Using discreet CV settings selectively transmitted either the light or heavy chain and corresponding proteoforms into the mass spectrometer for comprehensive analysis.
Comprehensive protein and proteoform characterization
Heavy Chain
Light ChainFull MS
CV -20
CV +40
CV +20
CV 0
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20000 30000 40000 50000 60000Mass (Da)
Thermo Scientific™ EASY-nLC™ 1200 system and the Thermo Scientific™ Orbitrap™ Eclipse™ Tribrid™ mass spectrometer. Ready-to-use method templates for the FAIMS Pro Interface simplify method setup.
2310050600 50800 51000 51200 51400 51600 51800 23200 23300 23400 23500
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ance
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G2FG2F+Hex
G0F-GlcNAc G2F+2Hex LC+Hex
LC
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Mass (Da)
N D I Q M T Q S P S T L S A S V G D R V T I T C S 25A26 S S R V G Y M H W Y Q Q K P G K A P K L L I Y D 50T51 S K L A S G V P S R F S G S G S G T E F T L T I 75S76 S L Q P D D F A T Y Y C F Q G S G Y P F T F G G 100G
101 T K V E I K R T V A A P S V F I F P P S D E Q L 125K126 S G T A S V V C L L N N F Y P R E A K V Q W K V 150D151 N A L Q S G N S Q E S V T E Q D S K D S T Y S L 175S176 S T L T L S K A D Y E K H K V Y A C E V T H Q G 200L201 S S P V T K S F N R G E C C
Light Chain (LC)
Mass (Da)
N Q V T L R E S G P A L V K P T Q T L T L T C T F 25S26 G F S L S T A G M S V G W I R Q P P G K A L E W 50L51 A D I W W D D K K H Y N P S L K D R L T I S K D 75T76 S K N Q V V L K V T N M D P A D T A T Y Y C A R 100D
101 M I F N F Y F D V W G Q G T T V T V S S A S T K 125G126 P S V F P L A P S S K S T S G G T A A L G C L V 150K151 D Y F P E P V T V S W N S G A L T S G V H T F P 175A176 V L Q S S G L Y S L S S V V T V P S S S L G T Q 200T201 Y I C N V N H K P S N T K V D K R V E P K S C D 225K226 T H T C P P C P A P E L L G G P S V F L F P P K 250P251 K D T L M I S R T P E V T C V V V D V S H E D P 275E276 V K F N W Y V D G V E V H N A K T K P R E E Q Y 300N301 S T Y R V V S V L T V L H Q D W L N G K E Y K C 325K326 V S N K A L P A P I E K T I S K A K G Q P R E P 350Q351 V Y T L P P S R E E M T K N Q V S L T C L V K G 375F376 Y P S D I A V E W E S N G Q P E N N Y K T T P P 400V401 L D S D G S F F L Y S K L T V D K S R W Q Q G N 425V426 F S C S V M H E A L H N H Y T Q K S L S L S P G C
Heavy Chain (HC)
Heavy Chain
CV +40Light Chain
CV -20
Guanidine adduct
Heavy Chain
Sodium adductGuanidine adduct
Light Chain
100
80
60
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20
0
Rel
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0
Comprehensive light and heavy chain characterization results at different CV settings using a HRAM MSn Orbitrap Eclipse Tribrid mass spectrometer method. The inset shows the deconvoluted MS spectrum with annotated glycoforms (heavy chain) and adducts (light chain). The increased selectivity provided by the FAIMS Pro interface enabled use of both wide and narrow quadrupole mass filters for subsequent top-down characterization using HCD, ETD, EThcD, and UVPD to generate complementary fragmentation. The sequence coverage map shows 45 and 65% coverage for the heavy and light chain, respectively. The red brackets represent c- and z- ions, blue brackets b- and y- ions, and green brackets a- and x- ions.
7
Enhanced top-down characterization
“ Using the FAIMS Pro interface on the Orbitrap Eclipse Tribrid MS significantly enhances the detection and deep characterization of proteoforms in either the direct infusion or LC-MS/MS modes of operation. Our data has generated a lot of excitement about using FAIMS, in part due to robustness of the ion source over time.”
Neil Kelleher Professor, Northwestern University
Enhanced targeted peptide quantification using selected reaction monitoringRoutine biomarker verification and validation studies require robust, precise, and accurate quantification across wide dynamic ranges and in the presence of complex biological matrices. Successful quantification at the LOQ and LLOQ is critical to establish values for early detection opportunities, but automated data processing becomes challenging due to matrix interference co-adding to the measured selected reaction monitoring (SRM) signal. When automated data processing becomes compromised, manual peak integration is required, resulting in reduced efficiency and increased chance of introducing bias. Incorporating the FAIMS Pro interface into existing nLC-SRM methods enhances selectivity, extends quantitative dynamic range, and permits confident automated data processing.
0.008
0.006
0.004
0.002
0.0000 5 10 15 20
attomols/uL25 30 35
0.008
0.006
0.004
0.002
0.0000 5 10 15 20
attomols/uL
Time (min) Time (min)
25 30 35
Standard Assay With FAIMS Pro Interface
Internal standard
Analyte
Internal standard
Analyte
0
50
100
31.06
31.0 31.5 32.0 32.5 33.0 33.5
30.99
31.31 31.66
31.8632.26
32.14
32.33
32.6333.10
32.9833.31
31.31 31.59
31.93
32.45 32.71 32.90 33.16 33.43
NL: 2.26E7
NL: 5.22E2
NL: 2.27E7
NL: 7.03E2
0
50
100
29.5 30.0 30.5 31.0 31.5
0
50
100
29.07 29.29
29.91
30.47 30.69 30.84 31.07 31.29
31.2431.1631.06
29.64
29.71
29.91
29.030
50
100
Are
a R
atio
Are
a R
atio
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The peak is selectively cleared from background noise in the FAIMS method and confirmed by SRM and retention time (RT) match with an internal standard. Using the FAIMS Pro interface (right panels) provides quantifiable signal at low concentration, which extends calibration curve linearity towards lower limits of detection (with FAIMS Pro interface: Slope = 2.02E-4; R2 = 0.9981, without FAIMS Pro interface: Slope = 1.94E-4; R2 = 0.9975).
Improved S/N and LOQs
Thermo Scientific™ TSQ Altis™ triple-stage quadrupole mass spectrometer has easy-to-operate and optimize methods for targeted quantitation with FAIMS Pro interface.
9
Quantification of Angiotensin I in digested plasma. Using FAIMS Pro interface with optimized CV value as part of the SRM table provides selective noise reduction allowing a quantifiable signal (right panels) even at concentration levels where analyte signal is impacted by noise interference in a standard run without using the FAIMS Pro interface (left panels).
Extended quantitative accuracy
Standard Assay With FAIMS Pro Interface
70 attomol
700 attomol
7 femtomol
700 femtomol
0
50
100
0
50
100
0
50
100
0
50
10011.04
10.39
10.5110.20 11.16 11.449.12 10.679.8011.869.719.47
10.08 11.04
10.38
10.48 10.64 11.589.82 11.319.14 11.719.55
10.06
11.0310.38 10.59 11.5811.309.829.12 11.849.589.31
10.04
10.32 11.0310.54 11.19 11.56 11.769.829.11 9.599.32
9.0 9.5 10.0 10.5 11.0 11.5 12.0Time (min) Time (min)
NL: 1.77E3
NL: 2.06E3
NL: 1.77E4
NL: 6.28E5
50
100
0
0
50
100
0
50
100
0
50
10010.20 11.54
11.1410.9710.71
11.429.32 9.71 11.63
9.879.14
10.18
11.5510.43 11.1310.719.869.35 11.699.509.16
10.16
10.34 10.97 11.5511.1110.62 11.819.68 9.809.18 9.38
10.16
10.42 10.69 11.5410.93 11.39 11.709.829.649.369.15
9.0 9.5 10.0 10.5 11.0 11.5 12.0
NL: 1.54E2
NL: 1.17E3
NL: 1.40E4
NL: 6.52E5
The instrument control software enables rapid CV evaluation and optimization. Thermo Scientific™ Orbitrap™ Tribrid™ Instrument Control software (version 3.1 or later), Thermo Scientific™ TSQ Instrument Control software (version 3.1 or later) and Thermo Scientific™ Orbitrap™ Exploris™ 480 Instrument Control software (version 1.0 or later) automatically recognize the FAIMS Pro interface assembly. In addition, the CV Scan Tool in the Tune application of the Instrument Control software can be used for infusion-based CV optimization for either total ion current (TIC), selected ion monitoring (SIM), or parallel reaction monitoring (PRM)-based detection. The resulting optimization files are automatically saved for further interrogation in Thermo Scientific™ Freestyle™ software.
Instrument tuning and optimization
Complex FAIMS Pro interface data are easily processed for high-level data interrogation. Thermo Scientific™ Proteome Discoverer™ software (version 2.3 or greater) manages data acquired at different CV settings and then merges the results into a concise, actionable report. In addition to detected and quantified proteins and peptides, processed results can also include biological functions, groupings, and pathway evaluations.
Automated data processing
Xcalibur software data acquisition methods
Predefined instrument method templates provide comprehensive, high-confidence data acquisition. The Method Editor application within the Instrument Control software provides flexibility to incorporate a variety of user-defined FAIMS Pro interface CV settings. The instrument method templates simplify complex data acquisition schemes by incorporating the optimized parameters needed to acquire high-quality MS and MS/MS data into a process requiring just a few mouse clicks.
With automated tuning and optimization, data-acquisition method templates, and streamlined data processing, setup and use of the FAIMS Pro interface with Thermo Scientific next-generation mass spectrometers are simplified, enhancing experimental performance and productivity regardless of user expertise. Seamless integration is designed to support maximum productivity for your most demanding science.
Everyday usability assures performance
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“ FAIMS provides up to 50% enhancement of identified peptides compared to LC-MS/MS analyses performed without FAIMS, and can extend the limit of detection by almost an order of magnitude. More importantly, the reduction in chimeric MS/MS spectra using FAIMS also improves the precision and the number of quantifiable peptides when using isobaric labeling of peptides.”
Pierre Thibault Principal Investigator, Proteomics and Bioanalytical Mass Spectrometry Research Unit, IRIC; Professor, Department of Chemistry, Faculty of Arts and Science, Université de Montréal. For more information about Thibault’s work with FAIMS see Molecular & Cellular Proteomics S. Pfammatter, et al. 2018, 17(10), pp. 2015–2067.
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The FAIMS Pro interface is compatible with a range of next-generation Thermo Scientific mass spectrometers, all of which deliver ease without sacrificing high performance. Combining the FAIMS Pro interface with the intelligent acquisition strategies that are built into the instrument control software enables parameter portability across multiple platforms for a true sample to knowledge workflow solution. Common capabilities include One-Click method set-up, Application mode with best-practice default parameters, and a common instrument method interface on the Orbitrap Tribrid, Orbitrap Exploris 480, and TSQ Triple Quadrupole mass spectrometers.
Integrated intelligence— a foundation for compatibility between systems
Orbitrap Tribrid mass spectrometers
Method EditorMethod Editor
TSQ Triple Quadrupole mass spectrometers
Orbitrap Exploris 480 mass spectrometers
Rapid method building with next-generation
method interface
Designed for experimental parameters to be transferable between systems
Unified architecture enables use of same performance accessories between
systems and easy parameter transfer
Scientifichypothesisgenerated
Target listgenerated
Large scalevalidationstudies
Method EditorMethod Editor
Rapid method building with next-generation
method interface
Method EditorMethod Editor
Rapid method building with next-generation
method interface
The FAIMS Pro interface is specifically compatible with:
• Thermo Scientific™ Orbitrap™ Exploris™ 480 mass spectrometer
• Thermo Scientific™ Orbitrap™ Eclipse™ Tribrid™ mass spectrometer
• Thermo Scientific™ Orbitrap™ Fusion™ Lumos™ Tribrid™ mass spectrometer
• Thermo Scientific™ Orbitrap™ Fusion™ Tribrid™ mass spectrometer
• Thermo Scientific™ TSQ Altis™ mass spectrometer
• Thermo Scientific™ TSQ Quantis™ mass spectrometer
• Thermo Scientific™ EASY-Spray™ Source
• Thermo Scientific™ Nanospray Flex™ ion source
• Thermo Scientific™ VeriSpray™ PaperSpray ion source
Find out more at thermofisher.com/FAIMSPro
For Research Use Only. Not for use in diagnostic procedures. © 2019 Thermo Fisher Scientific Inc. All rights reserved. Tandem Mass Tag and TMT are trademarks of Proteome Sciences plc. All other trademarks are the property of Thermo Fisher Scientific and its subsidiaries unless otherwise specified. This information is presented as an example of the capabilities of Thermo Fisher Scientific Inc. products. It is not intended to encourage use of these products in any manners that might infringe the intellectual property rights of others. BR65170-EN 1219M
Thermo Scientific™ Proteome Discoverer™ software thermofisher.com/ProteomeDiscoverer
Thermo Scientific™ BioPharma Finder™ software thermofisher.com/BioPharmaFinder
Thermo Scientific™ Xcalibur™ software thermofisher.com/Xcalibur
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