resveratrol and decreased fat deposition in c. elegans

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Resveratrol and Decreased Fat Deposition in C. elegans. Alexandra ******- Period 3 Manhasset Science Research. Need. Obesity Trends* Among U.S. Adults 1990, 1998, 2007. (*BMI  30, or about 30 lbs. overweight for 5’4” person). 1998. 1990. 2007. - PowerPoint PPT Presentation

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  • Resveratrol and Decreased Fat Deposition in C. elegansAlexandra ******- Period 3Manhasset Science Research

  • 1998

    Obesity Trends* Among U.S. Adults 1990, 1998, 2007(*BMI 30, or about 30 lbs. overweight for 54 person)20071990No Data

  • Knowledge BaseFigure 1: Chemical structure of Nile Red (A) & Sudan Black (B) staining. Nile Red staining in wild-type and mutant C. elegans (C-E). Sudan Black staining in wild-type (F) & mutant (G) organisms.Source: (Ashrafi, 2007) [http://www.wormbook.org/chapters/www_obesity/obesity.html]

  • ResveratrolFigure 2: chemical structure of resveratrol (C14H12O3)[http://www.3dchem.com/molecules.asp?ID=277] Chemical found in grapes; red wine Popular dietary supplement- advertised as anti-aging substanceMay explain French paradox

  • Literature ReviewFigure 2: RNAi was used to silence certain genes to determine which ones regulated fat in C. elegans (Ashrafi, et al, 2003) [chart made by author using materials from article]

  • Literature ReviewC. elegans in dauer need AMP-activated protein kinase (AMPK) to surviveDauer- larval hibernation stage in which metabolic rate is slowed; fat deposits increase to sustain organismAMPK- fat regulatory enzymeaak2 mutant dauers lack AMPK destroy fat(Roy, et al, 2009)

    Figure 3: The enzyme AMPK is necessary for C. elegans in dauer to survive and regulate fat deposition (Roy, et al, 2009) [chart created by author]

  • Literature ReviewResveratrol improves health and survival of mice on a high-calorie diet(Baur, et al, 2006)

    Figure 4: Resveratrol caused many positive changes in the overall health of mice similar to the effects of caloric restriction(Baur, et al, 2006) [chart made by author adapted from article]

  • Literature ReviewFigure 5: In human preadipocytes (fat cell precursors), resveratrol in extremely high doses (3-5 g) caused positive changes in the cells. (Fischer-Posovsky, et al, 2008) [chart made by author]

  • Purpose/HypothesisPurpose: to observe the effects of resveratrol on fat deposition in C. elegans and comparing it to caloric restrictionNull hypothesis: Resveratrol will cause no change in fat deposition in C. elegansAlternate hypothesis: Resveratrol will cause a decrease in fat deposition and mimic the effects of caloric restriction

  • Methodology

  • BudgetTotal Price: $198.69

  • Do-abilityResveratrol studies have been done in previous yearsC. elegans is a useful model organism that is easily obtainedSudan Black staining and fluorescent microscope examination are possible