REFERENCES

1. Ahmed IVP, Babu VS, Chandra V, Nambi KS, Thomas J, Ramesh B, Hameed ASS

(2009b) A new fibroblastic like cell line from heart muscle of the Indian major carp

(Catla catla): development and characterization. Aquaculture 293:180–186.

2. Ahmed IVP, Chandra V, Parameswaran V, Venkatesan C, Shukla R, Bhonde RR,

Hameed ASS (2008) A new epithelial-like cell line from eye muscle of catla (Catla

catla): development and characterization. J Fish Biol 72:2026–2038.

3. Ahmed IVP, Chandra V, Sudhakaran R, Rajesh Kumar S, Sarathi M, Sarath Babu V,

Ramesh B, Sahul Hameed AS (2009a) Development and characterization of cell lines

derived from rohu, Labeo rohita (Hamilton) and catla, Catla catla (Hamilton). J Fish Dis

32:211–218.

4. Akimoto K, Takaoka T, Sorimachi K (2000) Development of a simple culture method for

the tissues contaminated with microorganisms and application to establishment of a fish

cell line. Zool Sci 17:61–63.

5. Almeida-Toledo LF, Foresti F, Péquignot EV, Daniel-Silva MFZ (2001) XX:XY sex

chromosome system with X heterochromatinization: An early stage of sex chromosome

differentiation in the Neotropic electric eel Eigenmannia virescens. Cytogenet Cell Genet

95:73-78.

6. Alvarez MC, Otis J, Amores A, Guise K (1991) Short-term cell culture technique for

obtaining chromosomes in marine and fresh water. Jr Fish Biol 39: 817-824.

7. Arabi M (2004) Analyses of impact of metal ion contamination on Carp (Cyprinus

carpio) gill cell suspensions. Biological Trace Element Research 100: 229-245.

8. Avella M, Berhaut J, Payan P (1994) Primary culture of gill epithelial cells from the sea

bass, Dicentrarchus labrax. In Vitro Cell Dev Biol 30A:41-49.

9. Avise JC (1994) Molecular Markers: Natural History and Evolution. Chapman & Hall,

New York 511pp.

10. Babich H and Borenfl'eund E (1991) Cytotoxicity and genotoxicity assays with cultured

fish cells: a review. Toxicol. In Vitro 5: 91-100.

11. Babu VS, Chandra V, Nambi KSN, Majeed SA, Taju G, Patole MS, Hameed ASS,

(2012) Development and characterization of novel cell lines from Etroplus suratensis and

their applications in virology, toxicology and gene expression. Journal of Fish Biology

Volume 80, Issue 2: 312–334.

12. Bahich H and Borenfreund E (1991) Cytotoxicity and genotoxicity assays with cultured

fish cells: a review. Toxicol In Vitro 5:91–100.

13. Baker BI and Ingleton PM (1975) Secretion of Prolactin and Growth Hormone by Teleost

Pituitaries in vitro, Journal of Comparative Physiology 100:269–82.

14. Barnes D, Dowell L, Forest D, Parton A, Pavicevic P, Kazianis S (2006) Characterization

of XM a novel Xiphophorus melanoma-derived cell line. Zebrafish 3:371–381.

15. Bejar J, Borrego JJ, Alvarez MC (1997) A continuous cell line from the cultured marine

fish gilt-head seabream (Sparus aurata). Aquaculture 150:143–153.

16. Bejar J, Porta J, Borrego JJ, Alvarez MC (2005) The piscine 570 SAF-1 cell line: genetic

stability and labeling. Mar. Biotechnol 7: 389–395.

17. Benjamin M and Baker BI (1976) Ultrastructural Studies on Prolactin and Growth

Hormone Cells in Anguilla pituitaries in vitro, Cell and Tissue Research 174:547–64.

18. Birky CW, Fuerst P, Maruyama T (1989) Organelle gene diversity under migration,

mutation, and drift: equilibrium expectations, approach to equilibrium, effects of

heteroplasmic cells and comparison to nuclear genes. Genetics 121:613–627.

19. Bissett Jr. W, Smith L, Thompson JA (2009) Geostatistical analysis of DNA damage in

oysters, Crassostrea virginica, in Lavaca Bay, Texas. Ecotoxicology 18: 69-74.

20. Bols NC and Lee LEJ (1991b) Technology and uses of cell culture from tissues and

organs of bony fish. Cytotechnology 6:163–187.

21. Bols NC (1991a) Biotechnology and aquaculture: the role of cell cultures. Biotechnology

Advances 9: 31–49.

22. Bols NC, Dayeh VR, Lee LEJ, Schirmer K (2005) Use of fish cell lines in the toxicology

and ecotoxicology of fish. In: Moon, T.W.; Mommsen, T. P. (eds) Biochem. Molec. Biol.

Fishes. Vol. 6: 3-562.

23. Bols NC, Mosser DD, Steels GB (1992) Temperature studies and recent advances with

fish cells in vitro. Comp. Biochem. Physiol. 103A:1-14.

24. Braga D, Laize V, Tiago DM, Cancela ML (2006) Enhanced DNA transfer into fish bone

cells using polyethylenimine. Mol Biotechnol 34:51–54.

25. Brown WM (1983) Evolution of Genes and Proteins. In M Nei and RK Koehn, Sinauer,

Sunderland, Mass. Eds Evolution of Animal Mitochondrial DNA pp 62-88.

26. Brown WM (1985) The mitochondrial genome of animals: molecular evolutionary

genetics. In: MacIntyre RJ (ed) Molecular evolutionary genetics. Plenum Press New

York. pp 95–130.

27. Bruel G, Bonami JR, Pepin JF, Pichot Y Viral infection(picrona like viruses) associated

with mass mortalitiy in hatechry reared sea bass larvae and juviniels. Aquacultue 97:109-

116.

28. Bryson SP, Joyce EM, Martell JD, Lee LEJ, Holt SE, Kales SE, Fujiki K, Dixon B and

Bols NC (2006) A cell line (HEW) from embryos of haddock (Melanogrammus

aeglefinius) and its capacity to tolerate environmental extremes. Mar Biotechnol 8:641–

653.

29. Buonocore F, Forlenza M, Randelli E, Benedetti S, Bossu P, Meloni S, Secombes CJ,

Mazzini M, Scapigliati G (2006) Biological activity of sea bass (Dicentrarchus labrax L.)

recombinant interleukin-1 beta. Mar Biotechnol 7: 609–617.

30. Butler R, Nowak BF (2004) A dual enzyme method for the establishment of long and

medium-term primary cultures of epithelial and fibroblastic cells from Atlantic salmon

gills. J Fish Biol 65:1108–1125.

31. CAMP 1998 Report of the workshop ―Conservation, Assessmentand Management Plan

for Freshwater fishes of India 1997‖ organized by Zoo Outreach Organization (ZOO) and

National Bureau of Fish Genetic Resources, Lucknow, held at NBFGR in September

1997. 156pp.

32. Canli M (1995) Natural occurance of mettalothionein like proteins in the hepatopancreas

of the Norway lobster Nephrops norvegicus and effect of Cd, Cu, Zn exposure on level

on metal bound on metallothionin. Turkish Journal of Zoology 19: 313-321.

33. Carragher JF and Sumpter JP (1990) The effect of cortisol on the secretion of sex steroids

from cultured ovarian follicles of rainbow trout, Gen. Comp. Endo 77:403–07.

34. Castano A, Gomez-Lechon MJ (2005) Comparison of basal cytotoxicity data between

mammalian and fish cell lines: a literature survey. Toxicology In Vitro 1: 695–705.

35. Chang SF, Ngoh GH, Kuch LFS, Qin QW, Chen CL, Lam TJ, Sin YM (2001)

Developmental of a tropical marine fish cell line from Asian seabass (Lates calcarifer)

for virus isolation. Aquaculture 192:133-145.

36. Chen MJ, Chiou PP, Liao YH, Lin CM, Chen TT (2010) Development and

characterization of five Rainbow trout pituitary single-cell clone lines capable of

producing pituitary hormones. J Endocrinol 205:69–78.

37. Chen SL, Ren GC, Sha ZX, Shi CY (2004) Establishment of a continuous embryonic cell

line from Japanese flounder Pararlichthys olivaceus for virus isolation. Dis Aquat Organ

60:241–246.

38. Chen SL, Ren GC, Zhen-Xia S, Yunhan H (2005) Development and characterization of a

continuous embryonic cell line from turbot (Scophthalmus maximus). Aquaculture

249:63–68.

39. Chen SL, Sha ZX, Ye HQ (2003a) Establishment of a pluripotent embryonic cell line

from sea perch blastula embryo. Aquaculture 218:141–151.

40. Chen SL, Ye HQ, Sha ZX, Hong Y (2003b) Derivation of a pluripotent embryonic cell

line from red sea bream blastulas. J Fish Biol 63:795–805.

41. Chen TT & Powers DA (1990) Transgenic fish, Trends in Biotechnology 8: 209–15.

42. Chi SC, Hu WW , Lo BJ (1999a) Establishment and characterization of a continuous cell

line (GF-1) derived from grouper, Epinephelus coioides (Hamilton): a cell line

susceptible to grouper nervous necrosis virus (GNNV). Journal of Fish Diseases 22: 173-

182.

43. Chi SC, Lin SC, Su HM, Hu WW (1999b) Temperature effect on nervous necrosis virus

infection in grouper cell line and in grouper larvae. Virus Research 63: 107-114.

44. Chow S and Kishino H (1995) Phylogenetic relationships between tuna species of the

genus Thunnus (Scombridae: Teleostei): Inconsistent implications from morphology,

nuclear and mitochondrial genomes. Journal of Molecular Evolution 41:741-748.

45. Chow S, Okamoto H, Uozumi Y, Takeuchi Y, Takeyama H (1997) Genetic stock

structure of the swordfish (Xiphias gladius) inferred by PCR-RFLP analysis of the

mitochondrial DNA control region. Marine Biology 127:359–367.

46. Chung S & Secombes CJ (1988) Analysis of events occurring within teleost macrophages

during the respiratory burst, Comparative Biochemistry and Physiology B- Biochemistry

& Molecular Biology 89:539–44.

47. Ciba P, Schicktanz S, Anders E, Siegl E, Stielow A, Klink E, Kruse C (2008) Long-term

culture of a cell population from Siberian sturgeon (Acipenser baerii) head kidney. Fish

Physiol Biochem 34:367–372.

48. Cloud JG (1990) Strategies for introducing foreign DNA into the germ line of fish,

Journal of Reproduction and Fertility 41:107–16.

49. Collet B and Secombes CJ (2005) Construction and analysis of a secreting expression

vector for fish cells. Vaccine 23:1534–1539.

50. Cooper JK, Sykes G, King S (2007) Species identification in cell culture: a two-pronged

molecular approach. In Vitro Cellular & Developmental Biology 43: 344–351.

51. Dayeh VR, Lynn DH, Bols NC (2005) Cytotoxicity of metals common in mining effluent

to rainbow trout cell lines and to the ciliated protozoan, Tetrahymena thermophila.

Toxicology in Vitro 19:399-410.

52. Dayeh VR, Schirmer K, Bols NC (2002) Applying whole-water samples directly to fish

cell cultures in order to evaluate the toxicity of industrial effluents. Water Res. 36: 3727–

3738.

53. Dayeh VR, Schirmer K, Bols NC (2009) Ammonia-containing industrial effluents, lethal

to rainbow trout, induce vacuolization and Neutral Red uptake in the rainbow trout gill

cell line, RTgill-W1. ATLA 37(1):127-134

54. DeWitte-Orr SJ, Lepic K, Bryson SP, Walsh SK, Lee LEJ, Bols NC (2006) Development

of a continuous cell line, PBLE, from an American eel peripheral blood leukocyte

preparation. In Vitro Cell Dev Biol Anim 42:263–272.

55. Dinesh K, Nandeeshan M C, Nautiyal P, Aiyappa P (2010) Mahseers in India: A review

with focus on conservation and management. Indian Journal of Animal Sciences 80 (4)

(Suppl. 1): 26–38, April 2010.

56. Dirks WG, MacLeod RAF, Jaeger K, Milch H, Drexler HG (1999) First searchable

database for DNA profiles of human cell lines: sequential use of fingerprint techniques

for authentication. Cell Mol Biol 45:841–853.

57. Dodd NJF and Jha AN (2009) Titanium dioxide induced cell damage: A proposed role of

the carboxyl radical. Mutation Research – Fundamental and Molecular Mechanisms of

Mutagenesis. 660(1-2): 79-82.

58. Dong C, Weng S, Shi X, Shi N, He J (2008) Development of a mandarin fish Siniperca

chuatsi fry cell line suitable for the study of infectious spleen and kidney necrosis virus

(ISKNV). Virus Res 135:273–281.

59. Fahreaus-van Ree GE, Guldenaar SEF, Gielen J (1982) The Fine structure and function

of isolated gonadotropic cells as revealed from pituitaries of immature rainbow trout,

Salmo gairderi, by means of a new enzymatic dispersion technique, Cell and Tissue

Research 226:641–53.

60. Fahy GM (1988) Vitrification, In: Low temperature biotechnology, McGrath JJ & Diller

KR (Eds). The American Society of Mechanical Engineers, New York 113–46.

61. Faisal M and Ahne WH (1990) A cell line (CLC) of adherent peripheral blood

mononuclear leucocytes of normal common carp Cyprinus carpio, Developmental and

Comparative Immunology 8:255–60.

62. Faisal M, Rutan BJ, Sami-Demmerle S (1995) Development of continuous liver cell

cultures from the marine teleost, spot (Leiostomus xanthurus, Pisces: Sciaenidae).

Aquaculture 132:59–72.

63. Fan TJ, Jin LY, Wang XF (2003) Initiation of cartilage cell culture from skate (Raja

porasa Gunther). Mar Biotechnol 5: 64–69.

64. Faulmann E, Cuchens MA, Lobb CJ, Miller NW & Clem LW, (1983) An effective

culture system for studying in vitro mitogenic responses of channel catfish lymphocytes.

Transactions of the American Fisheries Society 112: 673–79.

65. Fengxia Tana, Min Wanga, Weimin Wanga, Yuanan Lua,b, (2008) Comparative

evaluation of the cytotoxicity sensitivity of six fish cell lines to four heavy metals in vitro

Toxicology in Vitro 22:164–170.

66. Fent K (2001) Fish cell lines as versatile tools in ecotoxicology: assessment of

cytotoxicity, cytochrome P4501A induction potential and estrogenic activity of chemicals

and environmental samples. Toxicol. In Vitro 15: 477–488.

67. Fernandez RD, Yoshimizu M, Kimura T, Ezura Y, Inouye K, Takami I (1993)

Characterization of three continuous cell lines from marine fish. Journal of Aquatic

Animal Health 5: 127–36.

68. Fishman MC (2001) Zebrafish the canonical vertebrate. Science 294:1290–1291.

69. Frerichs GN, Rodger HD, Peric Z (1996) Cell culture isolation of piscine neuropathy

nodavirus from juvenile sea bass, Dicentrarchus labrax. Journal of General Virology 77:

2067-2071.

70. Freshney RI (2000) Culture of Animal Cells: A Manual of Basic Technique, 4th Edition.

John Wiley and Sons. New Jersey.

71. Freshney RI (2005) Culture of animal cells: a manual of basic technique. Wiley and

Sons., New Jersey.

72. Fryer JL and Lannan CN (1994) Three decades of fish cell culture: A current listing of

cell lines derived from fishes. Methods in Cell Science. 16:2:87-94.

73. Fukuda Y, Nguyen HD, Furuhashi M, Nakai T (1996) Mass mortality of cultured

sevenband grouper, Epinephelus septemfasciatus, associated with viral nervous necrosis.

Fish Pathology 31:165-170.

74. Gold JR, Kristmunsdóttir AY, Richardson LR (1997) Mitochondrial DNA variation in

king mackerel (Scomberomorus cavalla) from the western Atlantic Ocean and Gulf of

Mexico. Marine Biology. 129:221-232.

75. Gong J, Huang Y, Huang X, Ouyang Z, Guo M, Qin Q (2011) Establishment and

characterization of a new cell line derived from kidney of grouper, Epinephelus akaara

(Temminck & Schlegel), susceptible to Singapore grouper iridovirus (SGIV), Journal of

Fish Diseases Volume 34, Issue 9: 677–686.

76. Goswami M , Lakra WS , Swaminathan TR , Rathore G (2010) Development of cell

culture system from the giant freshwater prawn Macrobrachium rosenbergii (de Man)

Mol Biol Rep 37:2043–2048.

77. Goswami M, Sharma BS, Tripathi AK, Yadav Kamalendra, Bahuguna SN, Nagpure NS,

Lakra WS, Jena JK (2012) Development and characterization of cell culture systems from

Puntius (Tor) chelynoides ,(McClelland) Gene Volume 500, Issue 1: Pages 140–147.

78. Guo H, Zhang S, Li H (2003) Spontaneous neoplastic transformation of the gill cell line

FG-3907 from the Olive Flounder Paralichthys olivaceus. N Am J Fish Aquaculture

65(1): 44-48.

79. Halton DM, Peterson WD Jr., Hukku B (1983) Cell culture quality control by rapid

isoenzymatic characterization. In Vitro 19:16–24.

80. Hameed ASS, Parameswaran V, Shukla R, Singh IS, Thirunavukkarasu AR, Bhonde RR

(2006) Establishment and characterization of India‘s first marine fish cell line from

kidney of sea bass, Lates calcarifer. Aquaculture 257:92–103.

81. Hartman A, Speit G (1994) Comparative investigations of the genotoxic effects of metals

in the single cell gel (SCG) assay and the sister chromatid exchange (SCE) test.

Environmental and Molecular Mutagenesis 23: 299-305.

82. Hebert PDN, Cywinska A, Ball SL, Waard, JR de (2003) Biological identifications

through DNA barcodes. Proceedings of the Royal Society of London B 270:313-322.

83. Hightower LH and Renfro JL (1988) Recent applications of fish cell culture to

biomedical research. J Exp Zool 248:290–302.

84. Himizu C, Hike H, Denise MM, Breisch E, Westerman M, Buchanan J, Ligman HR,

Phillips RB, Carlberg JM, Olst JV, Burns JC (2003) Characterization of a white bass

(Morone Chrysops) embryonic cell line with epithelial features. In Vitro Cell Dev Biol

Anim 39:29–35.

85. Hochachka P W and Mommsen TP (1994) Biochemistry and Molecular Biology of

Fishes Vol. 3: pp. 145–159.

86. Holen E, Kausland A, Skjærven K (2010) Embryonic stem cells isolated from Atlantic

cod (Gadus morhua) and the developmental expression of a stage-specific transcription

factor ac-Pou2. Fish Physiol Biochem doi:10.1007/ s10695-010-9381-z.

87. Hong Y, Chen S, Schartl M (2000) Embryonic stem cells in fish: current status and

perspectives. Fish Physiol Biochem 22:165–170.

88. Hong Y, Winkler C, Schartl M (1996) Pluripotency and differentiation of embryonic

stem cell lines from the medakafish (Oryzias latipes), Mechanisms of Development 60:

33–44.

89. Hsu YL, Yang YH, Chen YC, Tung MC, Wu JL, Engelking MH, Leong JC (1995)

Development of an in vitro subculture system for the Oka organ lymphoid tissue of

Penaeus monodon. Aquaculture 136:43–55.

90. Huang X, Huang Y, Sun J, Han X, Qin Q (2009) Characterization of two grouper

Epinephelus akaara cell lines: application to studies of Singapore grouper iridovirus

(SGIV) propagation and virus–host interaction. Aquaculture 292:172–179.

91. Imajoh M, Ikawa T, Oshima SI (2007) Characterization of a new fibroblast cell line from

a tail fin of red sea bream, Pagrus major and phylogenetic relationships of a recent RSIV

isolate in Japan. Virus Res 126:45–52.

92. Jesus CM, Galetti Jr. PM, Valentini SR, Moreira-Filho O (2003) Molecular

characterization and chromosomal localization of two families of satellite DNA in

Prochilodus lineatus (Pisces, Prochilodontidae), a species with B chromosomes. Genetica

118:25-32.

93. Jianrong Xu, Zhenmin Bao, Xiaolei Han (2011) A Novel Cell Line from Liver of Stone

Flounder, Kareius bicoloratus, and Its Susceptibility to Different Iridoviruses. Volume

42:1:73–81.

94. Kaattari SL, Irwin MJ, Yui MA, Tripp RA, Parkins JS (1986) Primary in vitro

stimulation of antibody production by rainbow trout lymphocytes. Immunology and

Immunopathology 12:29–38.

95. Kang MS, Oh MJ, Kim YJ, Kawai K, Jung SJ (2003) Establishment and characterization

of two cell lines derived from flounder, Paralichthys olivaceus (Temminck & Schlegel).

Journal of Fish Diseases 26: 657-665.

96. Kim GB and Lee RF (2004) Effects of genotoxic compounds on DNA and development

of early and late grass shrimp embryo stages. Marine Environmental Research. 57: 329-

338.

97. Knight CA, DeVries AL, Oolman LD (1984) Fish antifreeze protein and the freezing

and recrystallization of ice. Nature 38: 295–96.

98. Ku CC, Teng YC, Wang CS, Lu CH (2009) Establishment and characterization of three

cell lines derived from the rockfish grouper Epinephelus quoyanus: Use for transgenic

studies and cytotoxicity testing. Aquaculture 294:147–151.

99. Kuhnel D, Busch W, Meiszner T, Springer A, Potthoff A, Richter V, Gelinsky M,

Scholz S, Schirmer K (2009) Agglomeration of tungsten carbide nanoparticles in

exposure medium does not prevent uptake and toxicity toward a rainbow trout gill cell

line. Aquatic Toxicology 93(2-3):91-99.

100. Kumar GS, Singh IBS, Philip R (2001) Development of a cell culture system from the

ovarian tissue of African catfish (Clarias gariepinus). Aquaculture 194:51–62.

101. Kumar GS, Singh ISB, Rosamma P, Raveendranath M, Shanmugam J (1998) Efficacy

of fish and prawn muscle extracts as supplements to development of a primary cell

culture system from larval tissue of aquarium fish, Poecilia reticulate. Indian Jr Exp Biol.

36: 91-94.

102. Lai YS, Chiou PP, Chen WJ, Chen YC, Chen CW, Chiu IS, Chen SD, Cheng YH,

Chang CY (2008) Characterization of apoptosis induced by grouper iridovirus in two

newly established cell lines from barramundi, Lates calcarifer (Bloch). Journal of Fish

Diseases 31:825–834.

103. Lai YS, John JAC, Lin CH, Guo IC, Chen SC, Fang K, Lin CH, Chang CY (2003)

Estabilishment of cell lines from a tropical grouper, Epinephelus awoara (Temminck &

Schlegel), and their susceptibility to grouper irido–and nodaviruses. Journal of Fish

Diseases 26:31–42.

104. Lai YS, Murali S, Chiu HC, Ju HY, Lin YS, Chen SC, Guo IC, Fang K, Chang CY

(2001) Propagation of yellow grouper nervous necrosis virus (YGNNV) in a new

nodavirus susceptible cell line from yellow grouper, Epinephelus awoara (Temminck &

Schlegel), brain tissue. Journal of Fish Diseases 24:299–309.

105. Lai YS, Murali S, Ju HY, Wu MF, Guo IC, Chen SC, Fang K, Chang CY (2000) Two

iridovirus-susceptible cell lines established from kidney and liver of grouper,

Epinephelus awoara (Temminck & Schlegel), and partial characterization of grouper

iridovirus. Journal of Fish Diseases 23:379-388.

106. Lakra WS and Goswami M (2011) Development and characterization of a continuous

cell line PSCF from Puntius sophore. J Fish Biol.78:987-1001.

107. Lakra WS, Swaminathan TR, Rathore G, Goswami M, Yadav K, Kapoor S (2010a)

Development and characterization of three new diploid cell lines from Labeo rohita

(Ham.). Biotechnol Progr. 26:1008–1013.

108. Lakra WS, Swaminathan T Raja, Joy K P (2010b) Development, characterization,

conservation and storage of fish cell lines: a review Fish Physiol Biochem. 37, 1-20.

109. Lakra W S, Goswami M, Yadav Kamalendra , Gopalakrishnan A , Patiyal R S, Singh M

(2010c) Development and characterization of two cell lines PDF and PDH from Puntius

denisonii (Day 1865). In Vitro Cell.Dev.Biol.—Animal 47:89–94.

110. Lakra WS (2010d) Pluripotent embryonic stem cell line from catfish. ICAR News

16(1):3.

111. Lakra WS and Sarkar U K (2007) Fish diversity of Central India. NBFGR Publication,

Lucknow, Uttar Pradesh, pp 1–183.

112. Lakra WS, Bhonde RR, Sivakumar N and Ayyappan S (2006a) A new fibroblast like

cell line from the fry of golden mahseer Tor putitora (Ham). Aquaculture 253:238–243.

113. Lakra WS, Sivakumar N, Goswami M, Bhonde RR (2006b) Development of two cell

culture systems from Asian sea bass Lates calcarifer (Bloch). Aquacult Res 37:18–24.

114. Lakra WS and Bhonde RR (1996) Development of primary cell culture from the caudal

fin of an Indian major carp, Labeo rohita (Ham). Asian Fish Sci 9:149–152.

115. Laue C, Kaiser A, Wendl K (2001) Reversal of streptozotocindiabetes after

transplantation of piscine principal islets to nude mice, Transplantation Proceedings 33:

3504–10.

116. Lee LEJ, Caldwell SJ, Gibbons J (1997) Development of a cell line from skin of

goldfish, Carassius auratus, and effects of ascorbic acid on collagen deposition.

Histochemical Journal. 29:31-43.

117. Lee R and Kim GB (2002) Comet assays to assess DNA damage and repair in grass

shrimp embryos exposed to phototoxicants. Marine Environmental Research 54: 465-

469.

118. Lee RF, Kim GB, Maruya KA, Steinert SA, Oshima Y (2000) DNA strand breaks

(Comet assay) and embryo development effects in grass shrimp (Palaemonetes pugio)

embryos after exposure to genotoxicants. Marine Environmental Research 50:553-557.

119. Lee RF, Niencheski LFH, Brinkley K (2008) DNA strand breaks in grass shrimp

embryos exposed to highway runoff sediments and sediments with coal fly ash. Marine

Environmental Research 66:164-165.

120. Lee LEJ, Caldwell SJ, Gibbons J (1997) Development of a cell line from skin of

goldfish, Carassius auratus and effects of ascorbic acid on collagen deposition.

Histochemical Journal. 29:31-43.

121. Lee R and Kim GB (2002) Comet assays to assess DNA damage and repair in grass

shrimp embryos exposed to phototoxicants. Marine Environmental Research 54:465-469.

122. Lee RF, Kim GB, Maruya KA, Steinert SA, Oshima Y (2000) DNA strand breaks

(Comet assay) and embryo development effects in grass shrimp (Palaemonetes pugio)

embryos after exposure to genotoxicants. Marine Environmental Research 50: 553-557.

123. Lei XY, Chen ZY, He LB, Pei C, Yuan XP, Zhang QY (2012) Characterization and

virus susceptibility of a skin cell line from red-spotted grouper (Epinephelus akaara).

Fish Physiol Biochem. DOI: 10.1007/s10695-012-9603-7.

124. Leibovitz A (1963) The growth and maintenance of tissue-cell cultures in free gas

exchange with the atmosphere. Am J Hyg 78:173–180.

125. Lidgerding BC (1981) Cell lines used for the production of viral fish disease agents,

Develop. Biol. Standard 49: 233–41.

126. Lo CF, Leu JH, Ho CH, Chen CH, Peng SE, Chen YT, Yeh PY, Huang CJ, Wang CH,

Kou GH (1996) Detection of baculovirus associated with white spot syndrome (WSSV)

in penaeid shrimps using polymerase chain reaction. Dis. Aquat. Org. 25:133–141.

127. Loir M (1989) Trout Sertoli cells and germ cells in primary culture: I. morphological

and ultrastructural study, Gamete Research 24:151–69.

128. Loir M (1990) Trout steroidogenic testicular cells in primary culture I. Changes in free

and conjugated androgen and progestagen secretions: effects of gonadotropin, serum, and

lipoproteins. Gen. Comp. Endo 78:374–87.

129. Luc Rougee GK, Ostrander RH, Richmond YL (2007) Establishment, characterization,

and viral susceptibility of two cell lines derived from goldfish Carassius auratus muscle

and swim bladder. Dis Aquat Organ 77:127–135.

130. Lydersern BK, Pugh G, Paris MS, Sharma BP, Noll LA (1985) Ceramic matrix for large

scale animal cell culture. Bio/Technology 5:63–7.

131. Mantovani M, Abel LDS, Mestriner CA, Moreira-Filho O (2000) Accentuated

polymorphism of heterochromatin and nucleolar organizer regions in Astyanax

scabripinnis (Pisces, Characidae): Tools for understanding karyotypic evolution.

Genetica 109:161-168.

132. Matsuo Y, Nishizaki C, Drexler HG (1999) Efficient DNA fingerprinting method for the

identification of cross-culture contamination of cell lines. Hum Cell 12:149–154.

133. McKenna BM, Fitzpatrick RM, Phenix KV, Todd D, Vaughan LM, Atkins GJ (2001)

Formation of infectious pancreatic necrosis virus-like particles following expression of

segment A by recombinant semliki forest virus. Mar Biotechnol 3:103–110.

134. Meguro Y, Nakai T, Muroga, K, Sorimachi M (1991) A cell line derived from the fin of

Japanese Flounder, Paralichthys olivaceus. Fish Pathology 26(2):69-75.

135. Meyer P (1993) Expression and stability of foreign genes in animals and plants. In:

Release of Transgenic Organisms ed. K. Wöhrmann, Birkhäuser, Basel 5-23.

136. Miller NW & Clem LW (1984) Microsystem for in vitro primary and secondary

immunization of channel catfish (Ictalurus punctatus) leukocytes with hapten–carrier

conjugates, Journal of Immunological Methods 72:367–73.

137. Mori K, Nakai T, Muroga K, Arimoto M, Mushiake K, Furusawa I (1992) Properties of

a new virus belonging to Nodaviridae found in larval striped jack (Pseudocaranx dentex)

with nervous necrosis. Virology 187:368-371.

138. Munday BL, Langdon JS, Hyatt A, Humphrey JD (1992) Mass mortality associated with

a viral-induced vacuolating encephalopathy and retinopathy of larval and juvenile

barramundi, Lates calcarifer Bloch. Aquaculture 103:197-211.

139. Nicholson BL (1980) Growth of fish cell lines on microcarriers. Applied and

Environmental Microbiology 39:394–97.

140. Nicholson BL (1989) Fish cell culture: an update, Adv. Cell Culture 7: 1–18.

141. Nicholson BL, Danner DJ, Wu JL (1987) Three new continuous cell lines from marine

fishes of Asia. In Vitro Cellular & Developmental Biology 23:199-204.

142. Ogale SN 2002b Mahseer breeding and conservation and possibilities of commercial

culture. The Indian experience. In:Petr T and Swar D B. (eds.). Cold water fisheries in the

trans-Himalayan countries. FAO Technical Paper.No. 431 Rome,FAO. P.376.

143. Olivier G, Eaton CA, Campbell N (1986) Interaction between Aeromonas salmonicida

and peritoneal macrophages of brook trout (Salvelinus fontinalis), Veterinary

Immunology and Immunopathology 12:223–34.

144. Ossum CG, Hoffmann EK, Vijayan MM, Holt SE, Bols NC (2004) Characterization of a

novel fibroblast-like cell line from rainbow trout and responses to sublethal anoxia. J Fish

Biol 64:1103–1116.

145. Ozato K, Inoue K, Wakamatsu Y (1989) Transgenic fish: biological and technical

problems, Zoological Science 6: 445–57.

146. Palumbi S, Martin A, Romano S, McMillan W O, Stice L , Grabowski G (1991)The

Simple Fool‘s Guide to PCR. Version 2.0. Honolulu, HI: Department of Zoology and

Kewalo Marine Laboratory, University of Hawaii.

147. Parameswaran V, Ahmed VPI, Shukla R, Bhonde RR, Hameed ASS (2007)

Development and characterization of two new cell lines from milkfish (Chanos chanos)

and grouper (Epinephelus coioides) for virus isolation. Marine Biotechnology 9:281-291.

148. Parameswaran V, Shukla R, Bhonde RR, Hameed ASS (2006b) Establishment of

embryonic cell line from sea bass (Lates calcarifer) for virus isolation. Journal of

Virological Methods 137:309-316.

149. Parameswaran V, Shukla R, Bhonde RR, Hameed ASS (2006a) Splenic cell line from

sea bass, Lates calcarifer: establishment and characterization. Aquaculture 261:43–53.

150. Parameswaran V, Shukla R, Bhonde RR, Hameed ASS (2006c) Development of a

Pluripotent ES-like cell Line from Asian sea bass (Lates calcarifer)—an oviparous stem

cell line mimicking viviparous ES cells. Marine Biotechnology 9:766–775.

151. Pereira CSA, Guilherme SIAG, Barroso CMM, Verschaeve L, Pacheco MG, Mendo

SALV (2009) Evaluation of DNA Damage Induced by environmental exposure to

mercury in Liza aurata using the Comet assay. Archives of Environmental Contamination

and Toxicology 58:112-122.

152. Perry GML, McDonald GJ, Ferguson MM, Ganassin RC, Bols NC (2001)

Characterization of rainbow trout cell lines using microsatellite DNA profiling.

Cytotechnology 37:143–151.

153. Pettitt TR, Rowley AF, Secombes CJ (1989) Lipoxins are major lipoxygenase products

of rainbow trout macrophages, FEBS Letter 259:168–70.

154. Pombinho AR, Laize V, Molha DM, Marques SMP, Cancela LM (2004) Development

of two bone-derived cell lines from the marine teleost Sparus aurata; evidence for extra

cellular matrix mineralization and cell-type-specific expression of matrix Gla protein and

osteocalcin. Cell Tissue Res 315:393–406.

155. Powers DA (1989) Fish as model systems. Science 246:352-358.

156. Prasanna I, WS Lakra, SN Ogale, RR Bhonde (2000) Cell culture from fin explant of

endangered golden mahseer Tor putitora. Curr. Sci., 79: 93-95.

157. Qin QW, Wu TH, Jia TL, Hegde A, Zhang RQ (2006) Development and

characterization of a new tropical marine fish cell line from grouper, Epinephelus

coioides susceptible to iridovirus and nodavirus. J Virol Methods 131:58–64.

158. Rank J and Jensen K (2003) Comet assay on gill cells and haemocytes from the blue

mussel Mytilus edulis. Ecotoxicology and Environmental Safety 54:323-329.

159. Rao KS, Joseph M A, Shanker KM, Mohan CV (1997) Primary cell culture from

explants of heart tissue of Indian major carps. Current Science 73, 374-375.

160. Reeves JF, Davies SJ, Dodd NJF, Jha AN (2008) Hydroxyl radicals are associated with

titanium-dioxide nanoparticle-induced cytotoxicity and oxidative DNA damage in fish

cells. Mutation Research 640(1-2):113-122.

161. Reno PW and Nicholson (1980) Viral erytrotic necrosis (VEN) IN Atalantic cod invitro

studies.Can,J.Fish.Aquat.Sci 37:2276-2281.

162. Ristow SS and De Avila J (1994) Susceptibility of four salmonids cell lines to infectious

hematopoietic necrosis virus. J Aquat Anim Health 6:260–265.

163. Roberts FL (1970) Atlantic Salmon (Salmo salar) Chromosomes and Speciation,

Transactions of the American Fisheries Society 99: 105–11.

164. Rougee L, Ostrander GK, Richmond RH, Lu Y (2007) Establishment, characterization,

and viral susceptibility of two cell lines derived from goldfish Carassius auratus muscle

and swim bladder. Dis Aquat Org 77:127-135.

165. Sathe PS, Maurya DT, Basu A, Gogate SS, Banerjee K (1995) Establishment and

characterization of a new fish cell line, MG-3, from the gills of mrigal Cirrhinus mrigala.

Ind J Exp Biol 33:589–594.

166. Schirmer K, Dayeh, VR, Bopp S, Russold S, Bols NC (2004) Applying whole water

samples to cell bioassays for detecting dioxin-like compounds at contaminated sites.

Toxicology, 205: 211-221.

167. Schnell S, Bols NC, Barata C, Porte C (2009) Single and combined toxicity of

pharmaceuticals and personal care products (PPCPs) on the rainbow trout liver cell line

RTL-W1. Aquatic Toxicology 93(4):244-252.

168. Secombes CJ (1985) The in vitro formation of teleost multinucleate giant cells, Journal

of Fish Diseases 8: 461–64.

169. Servili A, Bufalino MR, Nishikawa R, de Melo IS, Munoz- Cueto JA, Lee LEJ (2009)

Establishment of long term cultures of neural stem cells from adult sea bass,

Dicentrarchus labrax. Comp Biochem Physiol Part A 152:245– 254.

170. Sha ZA, Ren G, Wang X, Wang N, Chen S (2010) Development and characterization of

a cell line from the embryos of half smooth tongue sole (Cynoglossus semilaevis). Acta

Oceanologica Sinica 29(2):81–87.

171. Sindermann C J (1990) Principal diseases of marine fish and shellfish, Academic Press.

San Diego.

172. Singh ISB, Rosamma P, Raveendranath M, Shanmugam J (1995) Development of

primary cell cultures from kidney of freshwater fish Heteropneustus fossilis. Indian

Journal of Experimental Biology 33: 595-599.

173. Siraj PB and Rani UA (2003) Cadmium induced antioxidant defense mechanism in

freshwater teleost Oreochromis mosssambicus (Tilapia). Ecotoxicology and

Environmental Safety 56, 218-221.

174. Song N and Toh H (1994) Membrane-bound Bacillus cytochromes c and their

phylogenetic position among bacterial class I cytochromes c. FEMS (Federation of

European Microbiological. Journal of Fish Diseases Volume 23, Issue 2 :161–169.

175. Sovenyi JF and Kusuda R (1987) Kinetics of in vitro phagocytosis by cells from head-

kidney of common carp, Cyprinus carpio. Fish Pathology 22: 83–92.

176. Stacey G, Bolton B, Doyle A, Griffiths B (1992) DNA fingerprinting--a valuable new

technique for the characterisation of cell lines. Cytotechnology 9 (1-3): 211-216.

177. Steube KG, Grunicke D, Drexler HG (1995) Isoenzyme analysis as a rapid method for

the examination of the species identity of cell cultures. In Vitro Cell Dev Biol Anim

31:115–119.

178. Stulberg CS, Peterson WD Jr, Simpson WF (1976) Identification of cells in culture. Am

J Hematol 1:237–242.

179. Sun L, Bradford CS, Ghosh C, Collodi P, Barnes DW (1995) ES-like cell cultures

derived from early zebrafish embryos, Molecular Marine Biology and Biotechnology

4:193–99.

180. Swaminathan TR, Lakra WS, Gopalakrishnan A, Basheer VS, Khushwaha B, Sajeela

KA (2010) Development and characterization of a new epithelial cell line PSF from

caudal fin of Green chromide, Etroplus suratensis (Bloch, 1790). In Vitro Cell Dev Biol

Anim. 46:647–656.

181. Takarada Y, Ojima Y, Takai A (1989) Establishment of a fish cell line transformed by

MNNG, RFM. Proc Japan Acad, 65 (B):108-111.

182. Tong SL, Lee H, Miao HZ (1997) The establishment and partial characterization of a

continuous fish cell line FG-9307 from the gill of flounder Paralichthys olivaceus.

Aquaculture 156:327–333.

183. Tong SL, Miao HZ, Li H (1998) Three new continuous fish cell lines of SPH, SPS and

RSBF derived from sea perch (Lateolabrax japaonicus) and red sea bream (Pagrosomus

major). Aquaculture 169:143–151.

184. Tort L and Torres P (1988) The effect of sublethal concentration of cadmium on

hematological parameters in dog fish, Scyliorhinus cannicula. Journal of Fish Biology

32:277-282.

185. Trust TJ, Kay WW, Ishiguro EE (1983) Cell surface hydrophobicity and macrophage

association of Aeromonas salmonicida, Current Microbiology 9:315–18.

186. Uma A, Prabhakar TG, Koteeswaran A, Ravikumar G (2002) Establishment of primary

cell culture from hepatopancreas of Penaeus monodon for the study of whitespot

syndrome virus (WSSV). Asian Fish Sci 15:365–370

187. Van der Kraak G (1991) Role of calcium in the control of steroidogenesis in

preovulatory ovarian follicles of the goldfish, General and Comparative Endocrinology

81:268–75.

188. Vishnyakova KS , Mugue NS , Zelenina DA , Mikodina EV , Kovaleva OA , Madan GV

, Yegorov YE (2009) Cell culture and karyotype of Sakhalin sturgeon Acipenser mikadoi

Biochemistry (Moscow) Supplemental Series A: Membrane and Cell BiologyVolume 3,

Number 1:42-54.

189. Wagg SK, Lee LEJ (2005) A proteomics approach to identifying fish cell lines.

Proteomics 5:4236–4244.

190. Wang G, LaPatra S, Zeng L, Zhao Z, Lu Y (2003) Establishment, growth,

cryopreservation and species of origin identification of three cell lines from white

sturgeon, Acipenser transmontanus. Methods Cell Sci 25:211–220.

191. Wang N, Wang XL, Sha ZX, Tian YS, Chen SL (2010b) Development and

characterization of a new marine fish cell line from turbot (Scophthalmus maximus). Fish

Physiol Biochem. doi: 10.1007/s10695-010-9402-y.

192. Wang R, Neumann NF, Shen Q, Belosevic M (1995) Establishment and characterization

of a macrophage cell line from the goldfish. Fish Shellfish Immunol 5:329–346.

193. Wang XL, Wang N, Sha ZX, Chen SL (2010a) Establishment, characterization of a new

cell line from heart of half smooth tongue sole (Cynoglossus semilaevis). Fish Physiol

Biochem. Fish Physio Biochem.36:1181-1189.

194. Ward RD, Zemlak TS, Innes BH, Last PR, Hebert PDN (2005) DNA barcoding

Australia's fish species. Philosophical Transactions of the Royal Society of London B

360:1847-1857.

195. Watanabe T, Nakano M, Aaskawa H, Moritomo T (1987) Cell culture of rainbow trout

liver. Nippon Suisan Gakkaishi. 53, 4:537-42.

196. Weil C, Hansen P, Hyam D, Le Gac F, Breton B, Crim LW (1986) Use of pituitary cells

in primary culture to study the regulation of gonadotropin hormone (GtH) secretion in

rainbow trout: Setting up and validating the system as assessed by its responsiveness to

mammalian and salmon gonadotropin releasing hormone, General and Comparative

Endocrinology 62: 202–09.

197. Wen CM, Lee CW, Wang CS, Cheng YH, Huang HY (2008) Development of two cell

lines from Epinephelus coioides brain tissue for characterization of betanoda virus and

megalocyti virus infectivity and propagation. Aquaculture 278:14–21.

198. Wergeland HI, Jakobsen RA (2001) A salmonid cell line (TO) for production of

infectious salmon anaemia virus (ISAV). Dis Aquat Organ 44:183–190.

199. White, GB (1973) Comparative studies on sibling species of the AnopheZes gambiae

Giles complex (Dipt., Culicidae). III. The distribution, ecology, behaviour and vectorial

importance of species D in Bwamba County, Uganda, with an analysis of biological,

ecological, morphological and cytogenetical relationships of Ugandan species D. Bull.

ent. Res. 63: 65-97.

200. Williams LM, Crane MStJ, Gudkovs N (2003) Development and characterisation of

pilchard (Sardinops sagax neopilchardus) cell lines derived from liver and heart tissues.

Methods Cell Sci 25:105–113.

201. Wilson AC, Cann RL, Carr SM, George M, Gyllensten UB, Helm-Bychowsiu KM,

Higuchi RG, Palumbi SR, Prager EM, Sage RD and Stoneking M (1985) Mitochondrial

DNA and two perspectives on evolutionary genetics. Biol J Linn Sot. 26:375-400.

202. Wise JP Sr, Winn RN, Renfro JL (2002) Generating new marine cell lines and

transgenic species. J Exp Zool 15:292–295.

203. Wittbrodt J, Shima A, Schartl M (2002) Medaka-A model organism from the Far East.

Nature Reviews Genetics 3:53–64.

204. Woelz J, Bork D, Witt G, Hollert H (2009) Ecotoxicological characterisation of

sediment cores from the western Baltic Sea (Mecklenburg Bight) using GC-MS and in

vitro biotests. Journal of Soils and Sediments 9(5):400-410.

205. Wolf K & Ahne W (1982) In: Maramorosch, K. (Ed.), Advances in Cell Culture, vol. 2.

Academic Press, New York: 305–328.

206. Wolf K & Mann JA (1980) Poikilotherm vertebrate cell lines and viruses: a current

listing for fishes. In Vitro 16:168–179.

207. Wolf K & Quimby MC (1962) Established eurythermic line of fish cells in vitro.

Science 135:1065–1066.

208. Wolf K & Quimby MC (1966) Fish cell and tissue culture. In: Hoar WS, Randall DJ

(eds) Fish physiology, vol III. Academic Press, New York, pp 253–305.

209. Wolf K & Quimby MC (1976a) Primary monolayer culture of fish cells initiated from

minced tissues. Tissue Culture Association Manual 2: 445-448.

210. Wolf K & Quimby MC (1976b) Procedures for subculturing fish cells and propagating

fish cell lines. Tissue Culture Association Manual 2:471-474.

211. Wolf K & Quimby MC (1976c) Primary monolayer culture of fish cells initiated from

trypsinized tissues. Tissue Culture Association Manual 2:453-457.

212. Wright JR Jr. & Yang H (1997) Tilapia Brockmann Bodies: an inexpensive, simple

model for discordant islet xenotransplantation, Annals of transplantation 2: 72–5.

213. Yadav K, Lakra WS, Sharma J, Goswami M, Singh A (2011) Development and

characterization of a cell line TTCF from endangered mahseer Tor tor (Ham.). Fish

Physiol Biochem. DOI 10.1007/s10695-011-9588-7.

214. Yan Y, Du J, Chen T, Yi M (2009) Establishment of medakafish as a model for stem

cell-based gene therapy: Efficient gene delivery and potential chromosomal integration

by baculoviral vectors. Exp Cell Res.; 315: 2322-2331.

215. Ye HQ, Chen SL, Sha ZX, Xu MY (2006) Development and characterization of cell

lines from heart, liver, spleen and head kidney of sea perch Lateolabrax japonicus. J Fish

Biol 69:115–126.

216. Zhang B, Wang X, Sha Z, Yang C, Liu S, Wang N, Chen SL (2011) Establishment and

Characterization of a Testicular Cell Line from the Half-Smooth Tongue Sole,

Cynoglossus semilaevis. Int J Biol Sci 7:452-459.

217. Zhao Y, Glesne D, Huberman E (2003) A human peripheral blood monocyte derived

subset acts as pluripotent stem cells. Proc Natl Acad Sci 100:2426–2431.

218. Zhao Z & Lu Y (2006) Establishment and characterization of two cell lines from bluefin

trevally Caranx melampygus. Dis Aquat Organ 68:91–100.

219. Zhao Z, Montgomery-Brock D, Lee CS, Lu Y (2003) Establishment, characterization

and viral susceptibility of 3 new cell lines from snakehead, Channa striatus (Blooch).

Methods Cell Sci. 25(3-4):155-66

220. Zhou GZ, Gui L, Li ZQ, Yuan XP, Zhang QY (2008) Establishment of a Chinese

sturgeon Acipenser sinensis tail-fin cell line and its susceptibility to frog iridovirus. J Fish

Biol. 73:2058–2067.

221. Zhou GZ, Li ZQ, Yuan XP, Zhang QY (2007) Establishment, characterization and virus

susceptibility of a new marine cell line from red spotted grouper (Epinephelus akaara).

Mar Biotechnol. 9:370–376.