Name: Redeat Belaineh ( DVM, MSc)Position: Associate researcer in molecular laboratory (NAHDIC)Department: Molecular biologyInstitute: National Animal health Diagnostic and Investigation CentreAddress and email: red.tkbr @yahoo.comCurrent study: Study on LSD vaccine efficacy using Kenyan SGPV and Neethling strains

Objectives:• To evaluate the protective efficacy of the currently used three vaccinal

strains (Neethling strain, Kenyan SGP O-180 strain and Gorgan goat pox strain) based on an experimental trail and sentinel herd monitoring study

• To carry out Molecular characterization of LSD virus circulating inEthiopia.

Methods: Preparation of experimental animals, molecular characterization of LSDV circulating in Ethiopia, Sentinel herd monitoring, Experimental design for vaccine challenge experiment , Clinical and serological observations post challenge and Data Analysis

Expected outputs from the project:• Contribute important scientific information for vaccine production

institutes• Contribute to produce better immunogenic CPV vaccines in the future • Economic losses due to LSD will be reduced.• To identify molecular characterization of LSD virus circulating in

Ethiopia, it gets opportunity to study further .

Impact of the outputs on agriculture in Africa:• If one of the transboundary diseases, Capripox disease could be

controlled livestock and livestock products trade will be improved .• Two different vaccines (Neethling strain and Kenyan SGPV ) have been

widely used for the control of LSD in cattle populations in southern and eastern Africa)(OIE, 2010).They will be benefited from this study .

The National Animal Health Diagnostic and Investigation Center (NAHDIC) is the referral and reference veterinarylaboratory in Ethiopia. It is the center of excellence for animal disease surveillance, investigation, diagnosis andresearch which contributes substantial role in promoting export of animal and animal products, improvement of thelivelihood of the smallholder farmers and pastoralists, provision of professional support for investors involved inanimal farming and transfer technology for stakeholders. The implementation of quality system in NAHDIC hasbrought an important milestone for NAHDIC in the face of regional and international community’s being as the EastAfrican supportive referral Diagnostic Laboratory for avian flu and Newcastle and other Trans boundary animaldiseases. This will have its own impact on international trade of animals and animal products from Ethiopia.Currently, NAHDIC has implemented ISO/IEC17025:2005 in three laboratories selecting 12 tests as its scope ofaccreditation since 2009. These tests have already accredited by accreditation body (SANAS). Has nine labs atBSL2 level, one at BSL3 level for the diagnosis of zoonotic diseases. The center is located in Sebeta 25kms from thecapital, Addis Ababa.

What types of molecular biology equipment do you have at your labs?

1. Agarose gel electrophoresis equipment 2. PCR thermocycler (Fast 7500 Applied biosystem ,

Biorad forr eal time PCR , Applied biosystem for conventional PCR and Lamp Temal cycler ( ESE –Melt)

3. PCR work station and safety cabinets class 24. Ice flack marker5. Laminar flow hood and chemical hood6. Wet ice maker 7. Liquid nitrogen 8. +40C fridge or cold room 9. -200C freezer 10. -800C freezer 11. Pipettes for volumes in the range 1 ul to 1 ml 12. -Spectrophotometer (UV/VIS) 13. Rotary incubator (370C) 14. Incubator cabinet (370C) 15. Autoclave16. pH meter17. Hot plate stirrer18. Magnetic stirrer 19. MilliQ pure water (or equivalent) 20. water distillater and Deionizer21. Different types of Centrifuge and vortex mixer22. Water bath23. Electronic balance24. Microwave oven25. Mini orbital shaker26. Easy access to reagents for molecular biology