recent discoveries in the prevention and control of brucellosis
TRANSCRIPT
RECENT DISCOVERIES IN THE
CONTROL OF BRUCELLOSIS
Presenters: 1. Dr. Moses Bwana
BVM, FELTP Intern
2. Dr. Gaturaga I.M
RVIL Mariakani
Venue: Sai Rock Hotel,
KVA Coast Branch Conference
Date: 14/8/2015
“The doctor of the future will give no medicine, but will
educate his patients in the care of the human frame,
in diet, and in the cause and prevention of disease.”Thomas Edison
THE PATHOGEN
Virulence linked to lifestyle.
*Metabolic mutant and nutrient shifter.
Quorum Sensing and/or starvation sensing
Best source of carbon Erythritol ( a Polyol, alcohol sugar).
Predilection sites: pregnant uterus, mature udder, testicles, accessory sex glands, joint space.
*Humans: Fever, sweat, headache, pain in muscles, joints and/or back.
EPIDEMIOLOGY
IP inversely proportional to stage of gestation at time of exposure (7days to 7 months)
Venereal transmission RARE
Transmission from bulls is mainly via intra-uterine A.I and contamination of pastures.
Wildlife species harbor the pathogen
Occupational hazard to animal handlers, slaughterhouse workers and lab personnel.
PREVENTION AND CONTROL
Why? No practical treatment is
available, best way forward.
Concept: “Brucella Free
Zones/Areas” (BFZ)
Where to start? Big Data
Where to get data? Surveillance
SURVEILLANCE
What is it? Continuous collection and analysis of animal health data to inform disease control programs.
Why? Pillar upon which a DFZ or BFZ can be sustained
Who? Livestock, humans and wildlife (One-Health)
Where? Entire country including coastal DFZ
When? Decide!
Objective? Provide data on Epidemiological knowledge, occurrence and distribution, support eradication and targeted interventions.
SURVEILLANCE contd…
How to prepare? -Case definition –risk factors –functioning vet field and lab capacity-sensitization and awareness of the public-animal health data management system-policy.
Surveillance sites: livestock markets, milk processing plants, slaughterhouses
Types of surveillance:
a. Passive surveillance ( reporting, mobile phones, leaflets, digital pens, field vets)
b. Active surveillance ( milk, syndromic, sero-surveillance, PDS and wildlife)
c. Outbreak investigations (confirmation of cases)
LABORATORY DIAGNOSIS
Why? To confirm presence or absence Precaution: Proper Biosafety and
biosecurity Basic assays: i. RBT and BRTii. BM ELISAiii. Indirect and Competitive ELISAiv. Culture and isolationv. FPA *MRVIL (RBPT and CFT)
MASS VACCINATION
As an emergency measure
To cover 80% of livestock
For 5-10 years
Identification of vaccinated
animals(brands, tags, tattoos, RFID)
Vaccines: S-19, RB-51, REV-1
Can we manufacture our own??
QUARANTINE AND
MOVEMENT CONTROL Affected animals and source herds
Stop movement orders
YOUNG STOCK
VACCINATION Young replacement females aged 4-8
months
Intra-caudal or intra-dermal route
TEST AND SLAUGHTER
Suitable when prevalence is below 1%
Public funds to compensate farmers
Appropriate management, animal
identification and monitoring of
vaccination coverage
BRUCELLOSIS INFORMATION
MANAGEMENT
Incidence reports (CVO)
Linked data bases between veterinary
and human health sectors
Brucellosis database applications
(ARIS-2)
One Health approach to outbreak
investigations.
CONTROL IN HUMANS
Relies heavily upon control in livestock
Biosafety and biosecurity in high risk
occupations.
Milk pasteuralisation
Proper cooking of meat
REFERENCES1. Ariza J, Bosilkovski, M. Caseio A, (2007): Perspectives for the Treatment of
Brucellosis in the 21st Century: The Ioannina Recommendations.
2. AU-IBAR, 2014: SMPS for Control of Brucellosis in the Greater Horn of Africa. Nairobi
3. Control of Brucellosis in Kuwait by Vaccination of Cattle, Sheep and Goats with Brucella abortus strain-19 or Brucella melitensis strain Rev-1
4. Diaz R. Casanova A. Ariza J. Moriyon, (2011): The Rose Bengal Test in Human Brucellosis: A Neglected Test fir the Diagnosis of a Neglected Disease.
5. Donev D, (2010): Brucellosis Control and Eradication in South Eastern European Countries: Currents Status and Perspective Strategies.
6. J. D. Anderson, H. Smith, (1965): The Metabolism of Erythritol by Brucella.
7. J. Lamontagne, A. Forest, E. Marazzo, (2009): Intracellular Adaptation of Brucella abortus.
8. Merck Sharp, (2009-2015): The Merck Veterinary Manual, Online Edition.
9. Russo G, (2009): Re-emergence of Human and Animal Brucellosis, Bulgaria.
10. Refai M, (2002): Incidence and Control of Brucellosis in the Near East Region
11. W. Eisenreich, T. Dandekar, (2010): Carbon Metabolism of Intracellular Bacterial Pathogens and Possible links to Virulence.
“He who conceals his disease cannot expect to be cured.”
Ethiopian Proverb.