rd international seminar on pharmaceutical science and...

3rdInternationalSeminaronPharmaceuticalScienceandTechnology[3rdISPST2018] Page

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Contents

ForewordfromChairmanoftheOrganizingCommittee……………………………………........ 18WelcomeSpeechfromDeanofFacultyofPharmacyUniversitasPadjadjaran…………. 19Committee……………………………………………………………………………………………………………...... 20ScientificProgramsandSchedule:

● Seminar………………………………………………………………………………………………………... 22● ParalelSession……………………………………………………………………………………………... 26

KeynoteSpeakers:

● Dra.EngkoSosialineMagdalene,Apt,M.BioMed,DirectorGeneralofPharmacyandMedicalDevices,MinistryofHealthRepublicofIndonesia(Indonesia)…..

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● Potential Use of Cyclodextrins as Multi-functional Drug Carriers and ActivePharmaceuticalIngredientsProf.HidetoshiArima(KumamotoUniversity,Japan)……………………………….........

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● NewMethodsfortheDevelopmentandStudyofDegradationofBiologicsVaccines:TherapeuticMonoclonalAntibodiesandInfluenzaVaccinesAssoc.Prof.VeyselKayser(UnivrsityofSydney,Australia)………………………………

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InvitedSpeakers: ● IntegratedapproachesinNaturalProductsResearchforDiscoveryofNew

ImmunomodulatoryAgents.Prof.Dr.Dato’IbrahimJantan(Malaysia).............................................................

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● DevelopmentofPharmaceuticalExcipientsforOralSolidDosageFormsfromLocalSourcesDr.rer.nat.AnisYohanaChaerunisaa,Apt.(Indonesia).......................................

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● BacterialCellulose-basedHydrogelasNewCellCarrierfortheTreatmentofFull-thicknessWoundInjuryProf.Dr.MohdCairulIqbalMohdAmin(Malaysia)…............................................

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● ConnectingPharmaceuticalScienceandClinicalPharmacyProf.Dr.YahdianaHarahap,M.S.,Apt(Indonesia)...............................................

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● Lignosusrhinocerotis(Cooke)Ryvarden(Tigermilkmushroom):AnAlternativefortheManagementofAllergicAirwayInflammationAssoc.Prof.NurulAsmaAbdullah,Ph.D.(Malaysia)............................................

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● 3D Structure-Ligand Based, ADME Prediction, and Molecular Dynamic of α-MangostinandItsDerivativesAgainstEstrogenReceptorαProf.Muchtaridi,M.Si.,Ph.D,Apt.(Indonesia)...................................................

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● Anewplant-baseddrugproductionplatformDr.NurKusairaBintiKhairulIkram(Malaysia)...................................................

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● Effect of Collaboration Product Development on Innovation to ImproveCompetitivenessandPerformanceofNationalPharmaceuticalCompanySubramaniParasuraman,M.Pharm.,Ph.D.(Malaysia).....................

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OralPresentation

Code Title Page

OP001 SynthesisOfMolecularImprintedPolymerForAtenololRecognitionUsingItaconicAcidAsFunctionalMonomerAliyaNurHasanah………………………………………………………………................

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OP006

ParticleDesignOfParacetamolBySphericalCrystallisationTechniqueIndra…………………………………………………………………..................................

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OP007 DurioZibethinus(Durian)FruitsWasteMediatedGreenSynthesisOfSilverNanoparticles:AGreenApproachV.Ravichandran……………………………………………………………….................

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OP008 InvestigationOnAntioxidantPropertyOfMalaysianGreenMusselsK.Venkateskumar………………………………………………………………...............

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OP011 SimultaneousVoltammetricDeterminationOfCaffeineAndParacetamolUsingCarbonPasteElectrodeModifiedWithCaffeineImprintedPoly(XylenolOrange)–ComparativeVoltammetricStudiesOfCaffeineRiskiaChandraWidianti………………………………………….............................

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OP012

DevelopmentAndValidationOfSimpleSimultaneousAnalysisForAmlodipineAndGlibenclamideByNon-DerivatizationHplc-FluorescenceFebrinaAmeliaSaputri………………………………………………………................

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OP013

FormulationAnti-AlopeciaOfAngiopterisEvectaExtractResmiMustarichie………………………………………………………………................

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OP014

SacranHydrogelFilmContainingEpidermalGrowthFactorForWoundHealingApplicationNasrulWathoni………………………………………………………………....................

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OP015 Effect Of Solvents And Maceration Time On Antioxidant Activity OfMangosteenPeel(GarciniaMangostanaL.)ExtractAndriKusmayadi……………………………………………………………….............

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OP017 Formulation Of Palm Shell Activated Charcoal(Elaeis Guineensis Jacg)ToothpasteAsTeethWhitenerInNicotineAddictsSyamsurizal………………………………………………………………..........................

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OP018 TestComparisonOfTheEffectivenessOfEmollientsFromPurePalmOilLotionsAndCrudePalmOilWithOilBaseInWaterUceLestari………………………………………………………………...........................

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OP020

VariationConcentrationOfCitricAcidAsAcidSourcesOnThePhysicalProperties Of The Pericarp Mangosteen (Garcinia Mangostana L)ExtractsEffervescentTabletIndingGusmayadi……………………………………………………………….................

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OP021

InVitroAntioxidantActivityOfGardenCroton (CodiaeumVariegatum(L.) Rumph. Ex A.Juss.) Using Dpph (2,2-Diphenyl-1-Picrylhydrazyl)MethodAndPhytochemicalAnalysisUsingGc-MsSitiWandaNurwanti……………………………………………………………...............

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OP022

Cytotoxic Activity of Voacanga Foetida (Bl.) K.Schum Leaves on T47dBreastCancerCellLineAndrianiSusanty………………………………………………………………..................

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OP023

ComparisonofCarbopol934and941asThickeneronDiffusionRateofKetoconazoleMicroemulsionRahmahElfiyani………………………………………………………………...................

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OP024

ProteaseActivityAndCharacterizationofBromelainExtractofPineapple(AnanasComusus(L.)Merr)CrownNyiMekarSaptarini……………………………………………………………….............

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OP025

DesignofIndicatorStripswithCelluloseBaseforDetectionofParacetamolinHerbalMedicineDriyantiRahayu………………………………………………………………....................

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OP026

RedGinger(ZingiberOfficinaleRoscoeVar.SuntiVal)ExtractsInhibitsIĸbaPhosphorylationinTheNfĸbInflammatoryPathwayofNeonatalRatCardiomyocytesSitiWakhidatunSuciyati………………………………………………………..............

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OP027

Antimicrobial Activity And Phytochemical Properties OfActinodaphneGlomerataLeavesExtractIneSuharyani………………………………………………………………......................

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OP028

Preliminary Test of Utilization of Aspergillus Niger in the Process ofBiotransformation of Geraniol and its Identification with GasChromatography-MassSpectrometri(Gc-Ms)YelfiAnwar………………………………………………………………..........................

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OP029

ApplicationofCommonGreenbottleFly(LuciliaSericata,Meigen1826)LarvaeExtractForIncisionWoundTreatmentsinRatsMadihah………………………………………………………………...............................

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OP030

FormulationandEvaluationofCaffeineAnti-CelluliteGelwithAdditionofGlycolicAcidasEnhancerYolaDesneraPutri………………………………………………………………...............

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OP031

EffectivityTestofEthanolAndWaterExtractsandDropsPreparationsofRedDragonFruitPeelasBoraxDetectorLilisAdeliah…………………………………………………………….............................

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OP032

AntioxidantActivityofΑ–AmyrinAndΒ-SitosterolfromBarkHexaneExtractsofRhizophoraMucronataLamk.Nohong………………………………………………………………................................

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OP033

CaffeineVoltammetricSensorBasedonElectropolymerizedMolecularlyImprintedPoly(GlutamicAcid)onCarbonPasteElectrodeNurAyuFitriani………………………………………………………………..............................

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OP034

AntiOxidantOfLimePeel(CitrusAurantifolia(Christm)Swingle)InEmulgelRahmadevi………………………………………………………………..........................

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OP036

ExplorationOfPotentialBioactiveCompoundsOfEndophyticMicrobialCultureIsolatedFromGallRustSengon(FalcatariaMoluccana)Barneby&J.WGrimesNoorRahmawati………………………………………………….............................

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OP038

PharmacophoreModelingAndMolecularDockingBasedVirtualScreeningOfB-CellLympoma2(Bcl2)InhibitorFromZincNaturalProductDatabaseFauzanZeinM.………………………………………………………………...................

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OP039

PotentialEthanolicExtractOfPasakBumi(EurycomaLongifolia)NanoparticlesAsAnAgentOfIncreasingArtemisininActivityOnPlasmodiumFalciparumDiahTriUtami………………………………………………………………......................

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OP040

ActiveCompoundsFromAmomumCompactumSol.ExMatonDedenIndraDinata……………………………………………………………….............

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OP041

Alpha Glucosidase Enzyme Inhibitory Activity of Okra (AbelmoschusEsculentus(L.)Moench)FruitWidhyaAligita……………………………………………………………….....................

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OP042

In-SilicoAnalysisAndHomologyModelingOfNs1AntigenIndonesiaOnDiagnosticSensitivityTestOfDengueDewiAstriany………………………………………………………………......................

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OP043

Isolation Of Antibacterial Compound From The Leaves Of MikaniaMicranthaH.B.KLiliAndriani………………………………………………………………..........................

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OP044

Comparison of Antibody Affinity Energy of Chk265 with Chk152 as aRapidDiagnosticComponentofAntiChikungunyaVirusKorryNovitriani………………………………………………………………...................

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OP045

AntiAgingPotentialOfMoringaExtractsNunukAriesNurulita……………………………………………………………...............

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OP047

Cyclodextrineglucanotransferase Production by Local Bacteria fromCassavaSoilDesiSagita………………………………………………………………...........................

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OP049

SedativeEffectofCitronella(CymbopogonNardus(L.)Rendle)TowardsMaleMice(MusMusculus)Yulianita……………………………………………………………….............................

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OP051

PharmacokineticInteractionsBetweenWarfarinandJavaneseTurmeric(CurcumaXanthorrhizaRoxb)ExtractinRatsTaofikRusdiana………………………………………………………………................

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OP052 Analysis of Lead Levels on Mascara Cosmetics from KiaracondongMarketUsingAtomAbsorptionSpectrophotometryMethodFentiFatmawati………………………………………………………………..................

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OP053

Stigmasterol of Ethyl Acetate Extract from Stem Bark of MelochiaUmbellata(Houtt)StapfVar.Visenia(Malvaceae)andDengueAntiviralActivityNunukHarianiSoekamto……………………………………………………….............

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OP054

Comparison of Bulk and Precipitation Polymerization Method ofSynthesisMolecularImprintedSolidPhaseExtractionforAtenololUsingMethacrylicAcidRimadaniPratiwi………………………………………………………………................

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OP057

PreliminaryAssessmentonCorrelationofFastingAnd2-HPostprandialGlucoseLevelChangeWithQualityofLifeOfType2DiabetesMellitusPatientUsingIndonesianEq-5d-5lValueSetDeniIskandar………………………………………………………………......................

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OP059

IsoflavoneAndAnthocyaninContentOfBlacksoybeanPromisingLinesDadangSumardi………………………………………………………………..................

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OP060

TotalFlavonoidsandAnthocyaninContentofPigmentedRiceRijantiRahajuMaulani…………………………………………………………...............

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OP061

Development and Characterization in Microencapsulation of Petai(ParkiaSpeciossaHassk.)LeafExtractsBuanasari………………………………………………………………............................

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OP063

SynthesisandMucoadhesiveStudyofNanoparticlesBasedonHydroxyPropylCellulose-CysteamineasaNovelNanomaterialDeniRahmat………………………………………………………………........................

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OP065

SynthesisandAntibacterialActivityTestsOfCurcuminAnaloguesUsingBromoFunctionalGroupIsmiRahmawati……………………………………………………………….................

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OP066

EffectOfPeg400,Peg4000AndPeg6000AsASuppositoriaBaseOnParacetamolDissolutionRateAlasenSembiringMilala……………………………………………………..............

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OP067

In-SilicoStudiesAndCharacterizationOfShortLinearLipopeptide-BasedTransfectionAgentPotentiallyCapableForNuclearExportTarwadi………………………………………………………………..............................

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OP068

ComputationalAntigenicEpitopePredictionofClinicalIndonesianDengueVirusNs1ProteinSabarPambudi………………………………………………………………....................

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OP069

Host-GuestInteractionsOfAlphaMangostinWith(Α,Β,AndΓ)−Cyclodextrins:SemiEmpiricalQuantumMechanicalMethodsOfPm6AndPm7DoniDermawan……………………………………………………………......................

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OP070

SpectrophotometricMethodforDeterminationofBoronContentinFoodWithPyridoxineComplexFormationAyuShalihat……………………………………………………………...........................

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OP071

ActivityofPorangFlourandMoringaExtracttoBloodGlucoseandLipidLevelsinAlloxanInducedDiabeticMiceYatiSumiyati…………………………………………………………….........................

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OP072 SynthesisandCharacterizationofLipopeptide-BasedTransfectionAgentasNon-ViralGeneDeliveryVehicleCandidateTarwadi……………………………………………………………..................................

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OP073

GelatinsasNaturalCompoundAndItsProportion-RatiowithGliptinstoDipeptidylAminopeptidaseIv(Dp-4)InhibitionYoniAtma……………………………………………………………..............................

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OP075

PhenolicCompoundsContentandAntioxidantActivityofElephantGinger(ZingiberOfficinaleRoscoe),RedGinger(ZingiberOfficinaleVarRubrum),andEmpritGinger(ZingiberOfficinaleVarAmarum)NurulMahmudati……………………………………………………………....................

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OP076

AntioxidantandAntiagingofNepheliumLappaceumPeelsExtractandItsCompoundsWahyuWidowati……………………………………………………………....................

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OP077

AntidiabetesEffectofElectrolyzedReducedWaterinType2DiabetesMellitusPatientsPoppyDiahPalupi……………………………………………………………...................

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OP078 IronEncapsulationUsingGlucomannanModifiedWithAceticAcidAsaMatrixDyahHestiWardhani…………………………………………………………….............

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OP080

InflammationStatusProfileInYoungAdultMenObese&NonObeseStudyOfHighSensitivityC-ReactiveProtein(Hscrp)SerumLevelAgusSulaeman…………………………………………………………….......................

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OP081 Uv-VisSpectroscopicMethodForSimultaneousEstimationOfCurcuminAndPiperineInDissolutionSamplesContainingCurcumaLongaAndPiperNigrumSolidDispersionDewiSetyaningsih……………………………………………………………………………

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0P082 PREPARATIONANDEVALUATIONOFTHEEFFERVESCENTGRANULESFROMBREADFRUIT(ArtocarpusAltilis(Parkinson.)Fosberg)LEAFETHANOLEXTRACTVinaFatimahOtuh……………………………………………………………………………

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PosterPresentationTuesday,23rdOctober2018

Code Title Page

PP-01 AntimicrobialActivityofSome IndonesianPlantSpeciesFromTheGenusFicus

DikiPrayugoWibowo................................................................................ 110

PP-02 Development, Characterization and Performance Evaluation OfTransferosomalGelof IbuprofenasATransdermalDrugDeliverySystemUsingNanovesicularCarrier

FitriantiDarusman..................................................................................... 111

PP-03 The Peel-off GelMask of Green Tea Leaf Extract (Camellia Sinensis L.) :FormulationandAntioxidantActivity

SetyoNurwaini............................................................................................ 112

PP-04 AcuteToxicityTestofEthanolExtractsofCawatHanoman(UrariaCrinitaL.)RootUsingOecd425Guidline

HelminaWati............................................................................................. 113

PP-06 Immunomodulatory Effect of Ethyl Acetate Leaf Extract of Permot(PassifloraFoetidaL.)AgainstTheSecretionofAntibodyandDelayedTypeHipersensitvityinVivo

AndiEmelda.............................................................................................. 114

PP-07 Dissolution Enhancement of Athorvastatin Calcium by Self NanoEmulsifying Drug Delivery Sytem Using Cremophor and Trascutol asSurfactants

SaniEgaPriani........................................................................................... 115

PP-08 Prospect of Christal Etil P-methoksisinamat Patch Properties Design OfKencurasanAlternativeDrugDeliverySystemAntiinflamation

HestiRiasari.............................................................................................. 116

PP-10 Topoisomerase Inhibitor Compound Isolated from N-hexane Fraction OfCempakaKuningStemBark(MicheliaChampacaL.)

AdeZuhrotun............................................................................................ 117


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PP-11 TheEffectivenessofin-situGelforChloramphenicolOpthalmicPreparationWithBasePoloxamer407andHpmcAgainstStaphylococcusAureusAtcc29213AndpseudomonasAeruginosaAtcc27853

InsanSunanKurniawansyah.................................................................

118

PP-12 ConstructionandExpressionofSyntheticGeneEncodingMpt64asExtracellularProteininEscherichiaColiBl21(De3)ExpressionSystem

SriAgungFitriKusuma.............................................................................. 119

PP-14 ChemicalCompositionOfEssentialOilOfStoneRedOnion(AlliumCepaL.)FromBayongbong,Garut,WestJava

RiaMariani................................................................................................. 120

PP-15 Utilization of Coconut Water and Dregs of Soy Sauce as Substrates InCarotenoidPigmentProduction

SenoAuliaArdiansyah................................................................................ 121

PP-16 Effect of Particle Size of Micro and Nano Ibuprofen Dissolution RateIbuprofenTabletwithDryGranulationMethod

FahjarPrisiska......................................................................................... 122

PP-17 Isolation-guided by in Vivo Antihypertensive Activity From Ethyl AcetateFractionofRoselle(HibiscusSabdariffaL.)

YasmiwarSusilawati................................................................................... 123

PP-18 AntiacnePotencyandGelFormulationFromPalmSugar (ArengaPinnataMerr.)AshSoxhletExtract

EndahRismawatiEkaSakti...................................................................... 124

PP-19 FormulationandAntibacterialActivityStudyofMouthwashContainingChitosanfromRajungan(PortunusPelagicus)CarapacePatihulHusni......................................................................... 125

PP-20 Rambutan Leaf Ethanolic Extract and Fraction (Nephelium Lappaceum)AgainstShigellaDysenteriaeAtcc13313andBacillusCereusAtcc1346asAntiDiarrheaAgent

Sulistiyaningsih......................................................................................... 126

PP-21 Antioxidant Activity from Ethanol Extract And Fraction HemigraphisColorataHall.F.LeavesUsingDpph

IrmaErikaHerawati................................................................................... 127

PP-22 AntibioticPotentialofTapakDara(CatharanthusRoseus(L.)G.Don)Leaf’sEtanolExtractToInhibitStreptococcusPyogenes

NitaArtiningsihSayekti............................................................................ 128

PP-24 AntifungalActivitySargassumAgainstCandidaAlbicans

OomKomala.............................................................................................. 129

PP-26 InhibitoryActivityofTheLeafEthanolicExtractAndFractionsofHibiscusSurattensisLonDipeptidylPeptidaseIV


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Yuliet............................................................................................................. 130

PP-28 SubacuteToxicityofCombinationC.XanthorrhizaandA.BlimbionLiverOfMaleWistarRats(RattusNorvegicusBerkenhout)

KartiawatiAlipin......................................................................................... 131

PP-29 AnalyticalMethodValidationOfAzadirachtinInTheNeemOilCreamUsingHplc

BellaPuteriIrinda...................................................................................... 132

PP-30 DevelopingNeem(AzadirachtaIndicaA.Juss)OilCreamFormulaas

Anti-scabies

NoriscaAlizaPutriana............................................................................... 133

PP-31 TheTestOfTabletDosagesMadeofPadinaAustralisasAnti-diarrhealOnMaleRatsStrainSpragueDawleyWhichIsInductedEschericiaColi

TriSaptariHaryani..................................................................................... 134

PP-32 Tablet Antibacterial Test Based on Padina Australis on Total BacteriaCausingDiarrhaeMaleRat(RattusNorvegicus)SpragueDawley

Triastinurmiatiningsih............................................................................... 135

PP-33 BrineShrimpLethalityBioassayAndPhytochemicalScreeningOfPrimates-consumedPlantsFromPangandaranBeachConservationArea

FerryFerdiansyahSofian........................................................................... 136

PP-35 Sunscreen Activity Testing of Robusta Coffee (Coffea Cenephora ExFroehner)LeaveExtractandFractions

KikiMulkiyaYuliawati................................................................................ 137

PP-36 VirtualScreeningNaturalCompoundsfromPlantsasInhibitorofEstrogenReceptorAlphaI(ESR1)

AndiTrihadiKusuma................................................................................. 138

PP-37 TyrosinaseInhibitorActivityMeasurementofCrudeandPurifiedExtractofMoringaLeaves(MoringaoleiferaL.)

ZainalAbidin.............................................................................................. 139

PP-38 StabilityOfVitaminCUnderInfluenceofRutinAdditionasAntioxidant

WinasihRachmawati.................................................................................. 140

PP-39 Formulation and Characterization of Self Nanoemulsifying Drug DeliverySystem(Snedds)ofFenofibricAcid

WiraNovianaSuhery.................................................................................. 141

PP-40 CostEffectivenessAnalysisoftheUseofaCombinationofAripiprazoleandEscitalopram with Aripiprazole and Agomelatine in Bipolar DisorderPatientsinOneofPharmaciesinBandung

YuliaWardati............................................................................................. 142


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PP-41 InvitroAntibacterialActivityofMelastomaCandidumLeafExractAgainstSomeCommonHumanPathogens

TitaJuwitaningsih........................................................................................ 143

PP-42 The Protective Effects of Red Dragon Fruit Juice Towards Doxorubicin-inducedTesticularToxicityinRats

BayuFebramPrasetyo.............................................................................. 144

PP-43 StudyofMolecularDocking,MolecularDynamicandToxicityPredictionOfSeveralQuinolineAlkaloidDerivativesasaBrutonTyrosinKinaseInhibitorasAntileukemia

FauzanZeinM.............................................................................................. 145

PP-44 TotalMonomericAnthocyaninPigmentContent inEthanolandMethanolButterflyPeaFlower(ClitoriaternateaL.)ExtractbypHDifferentialMethod

AnneYuliantini...........................................................................................

146

PP-45 Carica Papaya Leaves Reduced Blood Pressure and Arterial Stiffness OnAnimalModelHypertensionInducedbyHighFructoseDiet

Patonah................................................................................................. 147

PP-46 The Influence of Kerehau (Callicarpa longifolia Lamk.) Leaves Extract onLipidRatioofWistarMaleRat

ElisSusilawati............................................................................................. 148

PP-47 InfluenceOfMulberryLeafExtract(MorusAlbaL.)OnDiureticActivityOfMaleWhiteWistar-strainRat

DythaAndriDeswati.................................................................................... 149

PP-48 StudyOfSolidStateKineticsFromOneofMetastableEfavirenzduetoTheGrindingProcess

YogaWinduWardhana................................................................................ 150

PP-49 FormulationandinVitroReleaseStudiesofAnthocyaninfromPurpleSweetPotatoHydrophilicCreams

CokordaIstriSriArisanti............................................................................. 151

PP-50 TotalAnthocyaninsofPetalofRedRose(RosaDamasceneMill.)andRedChina Rose (Hibiscus Rosa-Sinensis L.) fromMaceration and PercolationMethods

GinayantiHadiseobroto................................................................................ 152

PP-52 FormulationandOptimazationofOrallyFastDissolvingFilmofBisoprololFumaratewithHpmcE15andMaltodextrinMatrix

AristhaNovyraPutri..................................................................................... 153

PP-53 HumanRedBloodCellsMembranStabilityPropertiesofZingiberOttensiVal.Extracts

LiaMarliani.................................................................................................... 154


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PP-54 EffectofEthanolExtractofArchidendronPauciflorumFruitPeelonCatalaseEnzyme Activity and Malondialdehyde Concentration in Streptozotocin-inducedDiabeticFemaleWistarRats(RattusNorvegicus)

DesakMadeMalini........................................................................................ 155

PP-55 PatternofAntiretroviral (Arv)DrugUse inOutpatient Prescriptions fromHiv/AidsClinicatOneofthePrivateHospitalinBandungCity

AniAnggriani............................................................................................. 156

PP-56 Alfa Glukosidase Inhibitory Activity in Vitro Assay of Leave, Flower, andRhizomeExtractsofEtlingeraElatior

R.HerniKusriani............................................................................................ 157

PP-57 AntibioticUseProfileInTheOupatientInAHospital

IdaLisni........................................................................................................ 158

PP-58 IsolationandEndophytesAntimicrobialActivitiesfromShiitakeMushroom(LentinulaEdodes)inBacteriaandFungi

IkaKurniaSukmawati................................................................................. 159

PP-61 Antibacterial Activity of Tespong (Oenanthe Javanica Dc), Sintrong(CrassocephalumCrepidioides),AndPohpohan(PileaTrinerviaW)AgainstBacteriaStaphylococcusEpidermidisandPseudomonasAeruginosa

AsepRoni.................................................................................................. 160

PP-62 Alpha-Glucosidase Inhibition Activity of Extract and Fractions Kupa(Syzygiumpolycephalum(Miq.)Merr.&Perry)Leaves

DadangJuanda............................................................................................ 161

Wednesday,24thOctober2018

Code Title Page

PP-64 StudyofCornstarchOriginatedfromLocalCornasBinderandDisintegrantinTabletsMarlineAbdassah......................................................................................... 162

PP-66 Skin Penetration Ability of Panthenol Gel With Ghk Cu Copper PeptideComplexes

YanniDhianiMardhiani............................................................................... 163

PP-67 MolecularDockingandAdmetPredictionofQuercetinAnalogsinDiscoveryofUrokinasePlasminogenActivatorInhibitors

BinaLohitaSari............................................................................................... 164

PP-68 PhysicochemicalCharacteristicsandQuantitativeAnalysisofS-allylCysteineofBlackGarlic

YoppiIskandar............................................................................................ 165


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PP-69 TheAntiobesity Effect of Ethanol Extracts andVarious Fraction ofMalayApple[SyzygiumMalaccense(L.)Merr&Amp;Perry]onHighCarbohydrateFoodandMonosodiumGlutamateInducedWistarRats

AtunQowiyyah............................................................................................. 166

PP-70 Evaluation of the Potency of Endophytic Fungi Extracts Associated withPotentiallyMedicinalPlantsfromMandalika-lombok,WestNusaTenggara

Praptiwi........................................................................................................ 167

PP-71 RadiolabelingofPlantaricinFwithIodine-131ForBioavailibilityStudyasaNaturalAntibiotic

EvaMariaWidyasari..................................................................................... 168

PP-72 Biodistribution of 99mtc-hsa-nanoparticle in Lymphatic Node of AnimalModelInducedBy7,12Dimethylbenz(A)Anthracene

RizkyJuwitaSugiharti................................................................................ 169

PP-73 AntioxidantActivityofEthanolicExtractandItsFractionsofPandanusTectoriusLeavesbyReductionInhibitionofWaterSolubleTetrazoliumSalt(Wst-1)Method

MoelyonoMoektiwardoyo........................................................................ 170

PP-74 PreparationofRutinLabeledScandium-46andOptimizationofItsTLCAnalysis

MuhamadBasitFebrian.............................................................................. 171

PP-75 XanthineOxidaseInhibitionActivityofGadungTuber(DioscoreaHispida),CommonPlantainLeaves(PlantagoMajorL.),ComfreyRoots(SymphytumOfficinaleL.),AsthmaWeedLeaves(EuphorbiaHirtaL.)

AmiTjitraresmi.............................................................................................. 172

PP-76 TheEffectofCeraAlbaConcentrationVariationsonthePhysicalStabilityoffBlackCumin(NigellaSativaL.)OilBalmStick

KoriYati........................................................................................................ 173

PP-77 AntioxidantActivityofThreeKindsofRiceBran(Red,BlackAndWhite)withDpphAssay

ZelikaMegaRamadhania............................................................................... 174

PP-78 Antibacterial Activity of Eleutherine Palmifolia L. Merr. Ethanolic ExtractAgainstBacillusCereus

ImamAdiWicaksono..................................................................................... 175

PP-79 OptimationofGeneEncodingAntiHer2Scfv[Pd861-pelb]OverexpressioninEscherichiaColiBl21(De3)

TinaRostinawati........................................................................................... 176

PP-80 Molecular Docking Studies and Virtual Screening of Compounds FromIndonesianNaturalProductsasPotentialNeuraminidaseH5n1InhibitorsRinaFajriNuwarda....................................................................................... 177


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PP-81 InVitroandinSilicoEvaluationofPhytoestrogeninProstateCancerGrowthInhibitory

RiniHendriani.............................................................................................. 178

PP-82 Glibenclamide-AscorbicacidCocrystalSynthesizedbyUsingtheSolventEvaporationMethodtoIncreasetheSolubilityofGlibenclamide

ArifBudiman................................................................................................. 179

PP-83 Effectivenes of Avocado Seeds (Persea AmericanaMill.) Extract Elixir forKidneyStones

SeptiaAndini................................................................................................ 180

PP-84 Synthesis and Characterization of Iodinated Rutin Through OxidationMethodUsingChloramine-t

MaulaEkaSriyani....................................................................................... 181

PP-85 Detection of Fish Allergens Prevalbumin Using Gel FiltrationChromatographyG-50andSds-page

EmmaEmawati............................................................................................ 182

PP-86 Study of Anti-aging Effectiveness and Irritation of Day Cream LoadingTetrahydrocurcumin

IkaYuniAstuti............................................................................................... 183

PP-87 Microwave-Assisted Extractionof FlavonoidCompounds fromOnion Skin(AlliumCepaL.)andAntibacterialActivityAgainstStaphilococcusAureus

TrirakhmaSofihidayati................................................................................ 184

PP-88 InSilicoStudyofPyrazolylaminoquinazolineToxicitybyLazar,Protox,andAdmetPredictor

Supandi......................................................................................................... 185

PP-89 BiologicalActivityandToxicityofAcetoneExtractsandSecondaryMetabolitesfromSoutheastSulawesiSponge,ChlatriaSp

I.Sahidin...................................................................................................... 186

PP-90 BiochemicalChangesintheCombinationofCaptoprilwithCeleryExtractInHypertensiveRatsInducedSodiumChloride

Siska.............................................................................................................. 187

PP-91 AdverseDrugReactionsProfileofFistLineAntiTuberculosisDrug(StudyOnIndonesianPharmacovigilansDatabase)

SetyoUtami................................................................................................. 188

PP-92 PotentialMedicationErrorinElectronicPrescriptionatPratamaHealthCareinBandung

RizkiSitiNurfitria.......................................................................................... 189

PP-93 Loop-mediated Isothermal Amplification (Lamp) for Rapid Detection OfSalmonellaSp.inTraditionalMedicineProducts

SoniMuhsinin.............................................................................................. 190


15

PP-94 StudytheLevelofKnowledgeofPregnantWomenAboutNon-prescriptionDrugsandTheirUseBehavior

NiNyomanSriMasHartini.......................................................................... 191

PP-95 ComparisonofAbelmoschusEsculentusandAbelmoschusMoschatusinDpphScavengingActivityandCytotoxicitytoMda-mb-231CancerCells

DianRatihLaksmitawati.............................................................................. 192

PP-97 ToxicityTestOfMahoniEthanolExtratc(Switeniamahagoni)

VirsaHandayani.......................................................................................... 193

PP-99 TheEffectofHclConcentrationintheHydrolysisProcessofNataDeCassavaontheCharacteristicsofMicrocrystallineCellulose

IGUSTINGURAHJEMMYANTONPRASETIA................................................

194

PP-100 Isolation and Antioxidant Activity of Secondary Metabolitesfrom PirdotLeaves(SaurauiavulcaniKorth.)

YumEryanti................................................................................................... 195

PP-102 The Activity Test of Acetone, Methanol and Ethanol Extract of MoringaOleiferaLeafasPhoto-ProtectionandInhibitorofActivityCollagenase

IinSuhesti.................................................................................................... 196

PP-103 PotencyofAngiopterisspp.ExtractasAntioxidant,Antibacterial, InhibitorofAcetylcholineesterase,andBeta-glucosidase

Praptiwi................................................................................................ 197

PP-104 Induction of Epithelial Ovarian Cancer by Implantation of 7,12-dimethylbenz(A)Athracene(Dmba)CoatedSilkintheOvaryofRatasAnimalModelforStudyAnticancerDrugsinVivo

NiMadeDwiSandhiutami....................................................................... 198

PP-105 CombinationKarasInfutionandAntibioticsAgainstPathogenicBacteria

RafikaSari............................................................................................... 199

PP-106 FICI Value Determination of Combination Aquilaria microcarpa Baill.EthanolicExtractwithAmoksisilinagainstUTIBacteria

PratiwiApridamayanti.......................................................................... 200

PP-107 FormulationandEvaluationofEnteric-coatedAspirinMicrocapsulesusingAcryl-eze®IibyExtrusionandSferonizationMethod

RahmatSantoso..................................................................................... 201

PP-108 Spectrophotometric Determination of Fucoidan From Water Extract ofSargassumAquifolium(Turner)C.Agradh

LielikNurhidayati.................................................................................... 202

PP-109 Developmentandvalidationof6-thioguanineand6-methylmercaptopurineinerythrocytesusingLiquidChromatographyTandemMassspectrometryanditsapplicationAcuteLymphoblasticLeukemiapatientstreatedwith6-mercaptopurine


16

DewiSelvinaRosdiana.................................................................................. 203

PP-110 Antibacterial Activity Of Pepaya Leaf (Carica Papaya Linn.) Extract AndFractionsAgaintsMethicillinResistantStaphylococcusAureus(Mrsa)ClinicalIsolatesAndStaphylococcusEpidermidisClinicalIsolates

TianaMilanda................................................................................................ 204

PP-111 EffectsOfDifferentPartsOfRedGuava(PsidiumGuajavaLinn.)OnSuperoxideDismutaseAndCatalaseActivities

AfiantiSulastri............................................................................................... 205

PP-112

Cost Analysis Of Type 2 Diabetes Mellitus In Outpatients WithoutComplicationAkhmadPriyadi............................................................................................. 206

PP-113

Optimization Of Nanoparticle Preparation Of Chitosan Human EpidermalGrowthFactorIonicGelationMethodWithAPolyethyleneGlycolStabilizerAsACandidateForDiabeticUlcerMedication

Sriwidodo....................................................................................................... 207

PP-114

Simple and Rapid Method for Analyzing Tamoxifen, Endoxifen, and 4Hydroxytamoxifen in Dried Blood Spot Using Liquid ChromatographyTandemMassSpectrometryBaithaPalanggatanMaggadani.................................................................... 208

PP-115

EffectofSilverNanoparticlesAddition inPeriodontalDressing forWoundTissueHealingBudhiCahyaPrasetyo.................................................................................... 209

PP-117

InclusionBodiesSolubilizationofCephalosporinAcylasefromEschrichiaColiDudiHaryanto........................................................................................... 210

PP-118 KnowledgeAndUsageofMedicinalPlantsbyLocalPeopleinWaigeoIsland,RajaAmpat,WestPapua,IndonesiaSitiSusiarti................................................................................................ 211

PP-119

PP-120

PP-121

PP-122

PP-123

In Silico StudyOn S-Allyl CysteineAndQuercetin Compound FromGarlic(AlliumSativum)AsXanthineOxidaseInhibitorAbdulAzizSetiawan................................................................................ OptimizationTransgeneExpressionofShortFullySynthesizedLipopeptide-BasedTransfectionAgentforNon-ViralGeneDeliveryVehicleDewiEstiRestiani...................................................................................... CloningandExpressionofOmpcGenefromSalmonellaTyphiinEschrichiaColiNadyaStephanie.......................................................................................ScreeningforAcetylcholinesteraseInhibitionofVegetablesfromIndonesiaMaulitaCutNuria.....................................................................................Hypoglycemic Activity of Ethanolic Extract and Fraction of Mengkudu(MorindaCitrifoliaL)FruitsinGlucoseInducedRats

212

213

214

215


17

PP-124

PP-125

PP-126

PP-127

PP-128

AhmadMuhtadi.........................................................................................CharacterizationofNaCmcHydrogelSynthesizedofHyacinthCelluloseIdaMusfiroh..............................................................................................QuantitativeStructure-ActivityRelationshipandMolecularDockingStudyofSeveralFlavonoidsasCox-2InhibitorsDadanSuryasaputra..................................................................................Antidiabetic Standard Medicines of Pisang Ranggap (Musa Paradisiaca)whichGrowsattheFootofMountGalunggung,TasikmalayaDolihGozali...............................................................................................EnhancingIntracellularTransportofRifampicinUsingAcemannanModifiedLipidNanoparticlesMuhammadLuthfiNugraha.......................................................................EnhancingIntracellularTransportofRifampicinUsingAcemannanModifiedLipidNanoparticlesSulistyaningsih...........................................................................................

216

217

218

219

220

221

Acknowledgment……………………………………………………………………………………………...........222


18

FOREWORD

Itgivesmegreatpleasure toextend toyouall averywarmwelcomeon theThird InternationalSeminar and Pharmaceutical Science and Technology 2018, here in Jatinangor, Indonesia.Thisseminarisacontinuationoffirsteventon2014andthesecondon2016.Thethemeofthisthirdseminaris“IntegrativeandInnovativeDrugDiscoveryandDevelopmentinPromotingGoodHealthandWell-BeingoftheUNSustainableDevelopmentGoals”.Asoneoftheinternationalevent,thescientificcontentof third ISPST2018 is trulymulti-disciplinary. It coversallaspectsofComputerAidedandDrugsDesign,AspectonIndustryandQualityControl,PharmaceuticalAnalysis,CurrentTrend in Drug Delivery Technology, Pharmacology and Toxicology, Cosmeceuticals, and CurrentTechnologyonDrugFormulation.Through sharing and networking, third ISPST 2018 will provide an opportunity for researchers,practitionersandeducators toexchangeresearchevidence,practicalexperiencesand innovativeideasonissuesrelatedtopharmaceuticalssciences.Werealizethatthiseventisonlyasmalleffortto contributeexchanges in researchesandexperiences, thereforemore collaborativeworksandcontinuousrelationshipbetweenresearchersarestillneeded.Third ISPST 2018 has received 198 abstracts addressing different scientific topics related topharmaceuticalscienceandtechnology.Thescientificprogramiscomposedof3keynotelecturers,4 plenary lectures from 8 lecturer, and198oral and poster presentations. The selected articlespresented in this seminarwill be publish in three international journal Scopus indexed and twonational journal Sinta indexed.The rich and diverse scientific contents represent many excitingachievementsandalsopointtonewchallenges.Onbehalfoftheorganizingcommittee,IwouldliketoextendawarmwelcometoalldelegatestoUniversitas Padjadjaran, Jatinangor, West Java, Indonesia. The organizers thank UniversitasPadjadjaran,sponsors,andoffcourseFacultyofPharmacyforgeneroussupporttothisinternationalseminar.It is a great privilege forme to be a Chairwoman and, in simple terms, it is the sheercommitmentofallcolleagueshereattheFacultyofPharmacytoprovidetheirverybestthatenablesustodeliverasophisticatedseminar.Welookforwardtoasuccessfulscientificevent,andwishourguestsanunforgettablemomentinWestJava.NyiMekarSaptariniChairwomanof3rdISPST2018


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WelcomeSpeechfromDeanofFacultyofPharmacyUniversitasPadjadjaran

It is a great pleasure that The Third International Seminar On Pharmaceutical Science AndTechnology 2018 (3rd ISPST 2018) organized by Department of Pharmaceutical Analysis andMedicinal Chemistry, Department of Pharmaceutical and Formulation Technology, andDepartmentofBiologicalPharmacy,FacultyofPharmacy,UniversitasPadjadjaranbeingheldonOctober23rdand24th2018. This seminar is amedia for researchers, students, industries, andpractitionerstoshareideasandlatestinformationonpharmaceuticalscienceandtechnologies.

Asweseeandfeelondailylife,theworldisnowfacingalotofpedicamentswhichseemtobehadtoovercomeunlesswejointhandsandtrytofightittogether.RecognitionofmultisectoralnatureofSDGs

Isencouragingdifferentstakeholdertogetinvolvedinwiderangeofactionandhealthisparticularlyactingasastrongdriverforpositivechange.Inthisseminarmanyresearchercomefromdifferentcountryanddifferentbackgroundtoshareideas.

The third ISPSTwill focused on integrative and innovative drug discovery and development toensurehealthylivesandpromotewellbeingforallatallagesasstatedinsustainabledevelopmentgoalsnumberthree.Thisseminarwillserveasavenueforresearcher,professional,andstudentswhich have interest in the area of pharmaceutical science and its related fields to buildmanycollaborationfortheirownresearchprojectandwillalsoenrichcollaborationsactivityineducation,researchandcommunityserviceofFacultyofPharmacyUniversitasPadjadjaran.

Ihopethisseminarwillaccomplishallitsaimsandearnestlydesirethatallparticipantswillbeabletobenefitfromthepresentationsanddisscusionsandthisseminarwillenrichthedevelopmentofpharmaceuticalscience,notonlyinIndonesiabutalsothroughoutAsiaasawhole.Iwouldliketothank the organizing committeewhose relentless efforts havemade this kind academic event areality. IwishallofthespeakersandparticipantswillgainmuchbenefitandhaveagoodtimeinJatinangor.Hopefullywewillmeetagaininthe4thISPSTinnexttwoyears.

BestRegards

Prof.Dr.AjengDiantini,M.Si.,Apt.


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Committee

DeanProf.Dr.AjengDiantiniM.S.(DeanofFacultyofPharmacyUniversitasPadjadjaran)AdvisoryBoard:1.Prof.Dr.AnasSubarnasM.Sc.2.Prof.Dr.MoelyonoM.W.3.Prof.Dr.AhmadMuhtadi4.Prof.Dr.ResmiMustarichie5.Prof.Dr.MarlineAbdassah,M.Si.6.Prof.Dr.JuttiLevita,M.S.7.Prof.Muchtaridi,M.Si.8.Dr.KeriLestariM.Si.Chairman:Dr.NyiMekarSaptarini,M.Si.ViceChairman:TaofikRusdiana,Ph.D.Dr.TianaMilanda,M.Si.Secretary:1. Dr.AliyaNurHasanah,M.Si.2. PatihulHusni,M.Si.3. ZelikaMega,M.Si.4. Dr.AdeZuhrotun,M.Si.5. DriyantiRahayu,M.T.6. RimadaniPratiwiM.Si.Treasurer:1. RinaFajriNuwarda,M.Sc.2. SorayaRatnawulanMita,M.SiScientificCommittee:1. NasrulWathoni,M.Si.,Ph.D.2. Dr.rer.nat.AnisYohanaChaerunissa,M.Si.3. Dr.YasmiwarSusilawati,M.Si.4.SriAgungFitriKusuma,M.Si.5.Dr.IyanSopyan,M.Si6.HolisAbd.Holik,M.Si7.NoriscaAlizaPutriana,M.Farm8.RanoKurniaSinuraya,MKM9.RiezkiAmalia,Ph.D


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Programme:1.Dr.IdaMusfiroh,M.Si2.FebrinaAmeliaSaputriPublication:1. SandraMegantara,M.Farm.2. RadenBayuIndradi,M.Si3.DindaAnjaniSponshorship:1. Sriwidodo,M.Si.2. DanniRamdhani,M.Si.3. YogaWindhuWardhana,M.Si.4. InsanSunanK,M.Farm.Logistic:1. Mutalin,P.hD.2. AnggaPrawira,M.Si.3. ImamAdi,M.Si.Consumption:1.Rr.SulistiyaningsihM.Kes.2.AmiTjitraresmi,M.Si.3.Dr.TinaRostinawati,M.SSupportingTeam:1.ArifBudiman,M.Si2.ArifSatriaWiraKusuma,M.Si.3.FerryFerdiansyah,M.Si.4.YuniElsaHadisaputri,S.Farm.,MBS,Ph.D.5.Dr.YoppiIskandar,M.Si


22

SCIENTIFICPROGRAMEANDSCHEDULE

Day1Tuesday,23thOctober2018

TIME LOCATION

07.30-08.00 Registration BaleSawala

08.00-09.00 OpeningCeremony:- TraditionalDance- WelcomeSpeech:1.RectorofUniversitasPadjadjaran2.DeanofFacultyofPharmacy3.ChairwomanoftheOrganizingCommittee

09.00–09.45 KeynoteSpeakerI:Prof.dr.NilaDjuwitaF.Moeloek,Sp.M*(MinistryofHealth,Indonesia)Dra.EngkoSosialineMagdalena,Apt.,M.Bio.Med.(DirectorGeneralofPharmacyandMedicalDevices)Moderator:Prof.Dr.AjengDiantiniM.S.,Apt

09.45-10.15 OpeningofExpoandOfficialExpoVisit,Coffebreak BaleSawala

10.15-11.00 KeynoteSpeakerII:Prof.HidetoshiArima,Ph.D.(KumamotoUniversity,Japan)“PotentialUseofCyclodextrinsasMulti-functionalDrugCarriersandActivePharmaceuticalIngredients”Moderator:NasrulWathoniPh.D.,Apt

BaleSawala

11.00-12.00 InvitedSpeakerI:Prof.Dr.YahdianaHarahap,M.S.,Apt.(IndonesiaUniversity,Indonesia)“ConnectingPharmaceuticalScienceandClinicalPharmacy”InvitedSpeakerII:Prof.Dr.Dato’IbrahimJantan(UniversitiKebangsaanMalaysia)“IntegratedapproachesinNaturalProductsResearchforDiscoveryofNewImmunomodulatoryAgents”Moderator:Prof.Dr.MoelyonoMW.,M.S.,Apt

12.00-13.00 LunchBreak,PosterSessionandExpoVisit BaleSawala

13.00-14.00 InvitedSpeakerIII:Assoc.Prof.NurulAsmaAbdullah,Ph.D.(UniversitiSainsMalaysia)“Lignosusrhinocerotis(Cooke)Ryvarden(Tigermilkmushroom):AnAlternativefortheManagementofAllergicAirwayInflammation”

BaleRucita


23

InvitedSpeakerIV:Prof.Dr.Muchtaridi,M.Si.,Apt(UniversitasPadjadjaran,Indonesia)“3DStructure-LigandBased,ADMEPrediction,andMolecularDynamicofα-MangostinandItsDerivativesAgainstEstrogenReceptorα”Moderator:Dr.TinaRostinawatiM.Si.,Apt

14.00–15.45 ParalelSession R.1:BaleSawalaR.2:BaleRucitaR.3:DRPM4thFloorR.4:BaleRancage1,4thFloorR.5:MeetingRoom,2ndFloor

15.45-16.00 CoffeeBreak,PostersessionandExpoVisit


24

Day2Wednesday,24thOctober2018

TIME LOCATION

08.00-08.45 KeynoteSpeakerIII:Assoc.Prof.VeyselKayser(UniversityofSydney,Australia)“NewMethodsfortheDevelopmentandStudyofDegradationofBiologicsVaccines:TherapeuticMonoclonalAntibodiesandInfluenzaVaccines”Moderator:Prof.ResmiMustarichiePh.D.,Apt

BaleSawala

08.45-09.45 InvitedSpeakerV:Prof.Dr.MohdChairulIqbalMohdAmin(UniversitiKebangsaanMalaysia)“BacterialCellulose-basedHydrogelasNewCellCarrierfortheTreatmentofFull-thicknessWoundInjury”InvitedSpeakerVI:Dr.NurKusairaBintiKhairulIkram(UniversityofMalaysia)“Anewplant-baseddrugproductionplatform”Moderator:Dr.SandraMegantaraM.Farm.,Apt

09.45-10.15 CoffeeBreak,PostersessionandExpoVisit

10.15-11.15 InvitedSpeakerVII:SubramaniParasuraman,M.Pharm.,Ph.D.(AIMSTUniversity,Malaysia)“EffectofCollaborationProductDevelopmentonInnovationtoImproveCompetitivenessandPerformanceofNationalPharmaceuticalCompany”InvitedSpeakerVI:Dr.rer.nat.AnisYohanaChaerunisa,M.Si.,Apt(UniversitasPadjadjaran,Indonesia)“DevelopmentofPharmaceuticalExcipientsforOralSolidDosageFormsfromLocalSources”Moderator:HolisAbd.HolikPh.D.,Apt

BaleSawala

11.15–12.15 HerbalCornerPresentation BaleSawala

12.15–13.15 LunchBreak,PosterSessionandExpoVisit

13.30-15.15 ParalelSession R.1:BaleSawalaR.2:BaleRucitaR.3:DRPM4thFloorR.4:BaleRancage1,4thFloor


25

R.5:MeetingRoom,2nd

Floor

15.15–15.45 Coffeebreak,PosterSessionandExpoVisit

15.45–16.00 ClosingCeremony

*scheduleforparallelsessioncanbeseenonseparatepage


26

TimeScheduleforParalelSession

Tuesday,23rdOctober2018R.1BaleSawalaModerator:DriyantiRahayuMT.Time Kode Name Title14.00-14.15 OP001 AliyaNurHasanah SYNTHESISOFMOLECULARIMPRINTEDPOLYMERFOR

ATENOLOLRECOGNITIONUSINGITACONICACIDASFUNCTIONALMONOMER

14.15-14.30 OP007 Dr.V.RAVICHANDRAN

DURIOZIBETHINUS(DURIAN)FRUITSWASTEMEDIATEDGREENSYNTHESISOFSILVERNANOPARTICLES:AGREENAPPROACH

14.30-14.45 OP011 RiskiaChandraWidianti

SIMULTANEOUSVOLTAMMETRICDETERMINATIONOFCAFFEINEANDPARACETAMOLUSINGCARBONPASTEELECTRODEMODIFIEDWITHCAFFEINEIMPRINTEDPOLY(XYLENOLORANGE)–COMPARATIVEVOLTAMMETRICSTUDIESOFCAFFEINE

14.45-15.00 OP037 MuhammadNurAbdillah

ISOLATIONANDSCREENINGOFX1ANDX2XYLANASE-PRODUCINGBACTERIAFROMPEUYEUMSINGKONG(CASSAVA)(ManihotesculentaCranzt)USINGPCR(POLYMERASECHAINREACTION)

15.00-15.15 OP049 Yulianita SEDATIVUMACTIVITYOFCITRONELLA(Cymbopogonnardus(L.)Rendle)TOWARDSMALEMICE(Musmusculus)

15.15-15.30 OP063 DeniRahmat SYNTHESISANDMUCOADHESIVESTUDYOFNANOPARTICLESBASEDONHYDROXYPROPYLCELLULOSE-CYSTEAMINEASANOVELNANOMATERIAL

15.30-15.45 OP072 DrsTarwadi,MSc in-silicostudiesandcharacterizationofshortlinearLipopeptide-basedTransfectionAgentPotentiallyCapableforNuclearExport

R.2BaleRucitaModerator:SriAgungFitriKM.Si.,AptTime Kode Name Title14.00-14.15 OP015 AndriKusmayadi,

M.Sc.EFFECTOFSOLVENTSANDMACERATIONTIMEONANTIOXIDANTACTIVITYOFMANGOSTEENPEEL(GarciniaMangostanaL.)EXTRACT

14.15-14.30 OP012 FebrinaAmeliaSaputri

DEVELOPMENTANDVALIDATIONOFSIMPLESIMULTANEOUSANALYSISFORAMLODIPINEANDGLIBENCLAMIDEBYNON-DERIVATIZATIONHPLC-FLUORESCENCE


27

14.30-14.45 OP040 DedenIndraDinata

ACTIVECOMPOUNDSFROMAmomumcompactumSol.ExMaton

14.45-15.00 OP041 WidhyaAligita ALPHAGLUCOSIDASEENZYMEINHIBITINGACTIVITYOFOKRA(AbelmoschusEsculentus(L.)Moench)FRUIT

15.00-15.15 OP043 LILIANDRIANI ISOLATIONOFANTIBACTERIALCOMPOUNDFROMTHELEAVESOFMIKANIAMICRANTHAH.B.K

15.15-15.30 OP044 KorryNovitriani COMPARISONOFANTIBODYAFFINITYENERGYOFCHK265WITHCHK152ASARAPIDDIAGNOSTICCOMPONENTOFANTICHIKUNGUNYAVIRUS

15.30-15.45 OP027 IneSuharyani,M.Si.,Apt.

ANTIMICROBIALACTIVITYANDPHYTOCHEMICALPROPERTIESOFACTINODAPHNEGLOMERATALEAVESEXTRACT

R.3DRPM4thFloorModerator:RiezkiAmeliaPh.D.,Apt

Time Code Title

14.00-14.15 OP048 IrEndangNoerhartati.,M.P

IMMUNOMODULATORYACTIVITYOFREDSORGHUMROOTEXTRACTAGAINSTTNF-AINMICEMODELSIMMUNOCOMPROMISED

14.15-14.30 OP024 NyiMekarSaptarini

PROTEASEACTIVITYANDCHARACTERIZATIONOFBROMELAINEXTRACTOFPINEAPPLE(Ananascomusus(L.)Merr)CROWN

14.30-14.45 OP050 WahyuHidayati,S.Si.,M.Biomed

APreliminaryStudyofAntibacterialandAntidiabetesPotentialofEndophyticFungiFromIndonesianBelimbingWuluh(AverrhoabilimbiL.)Fruits

14.45-15.00 OP057 DeniIskandar PRELIMINARYASSESSMENTONQUALITYOFLIFEOFTYPE2DIABETESMELLITUSPATIENTUSINGINDONESIANEQ-5D-5LVALUESETANDITSCORRELATIONWITHBLOODGLUCOSELEVEL

15.00-15.15 OP008 KVENKATESKUMAR

INVESTIGATIONONANTIOXIDANTPROPERTYOFMALAYSIANGREENMUSSELS

15.15-15.30 OP016 EndangSetyowati,MSc.,Apt

ClinicalTrialofHerbalProductsCombinationofExtractGynuraprocumbensLourMerrLeafandCurcumaXanthorrhizaRoxbRhizomeasAntiDyslipidemia

15.30-15.45 OP074 AlfanDannyArbianto

ComputationalStudiesforDesigningLipopeptideasTransfectionAgent


28

R.4BaleRancage1,4thFloorModerator:TaofikRusdianaPh.D.,Apt

Time Code Title

14.00-14.15 OP006 INDRAM.Si PARTICLEDESIGNOFPARACETAMOLBYSPHERICALCRYSTALLISATIONTECHNIQUE

14.15-14.30 OP013 ResmiMustarichie FORMULATIONANTI-ALOPECIAOFANGIOPTERISEVECTAEXTRACT

14.30-14.45 OP014 NasrulWathoni SacranHydrogelFilmContainingEpidermalGrowthFactorforWoundHealingApplication

14.45-15.00 OP017 Syamsurizal FORMULATIONOFPALMSHELLACTIVATEDCHARCOAL(ElaeisguineensisJacg)TOOTHPASTEASTEETHWHITENERINNICOTINEADDICTS

15.00-15.15 OP018 UceLestari TESTCOMPARISONOFTHEEFFECTIVENESSOFEMOLLIENTSFROMPUREPALMOILLOTIONSANDCRUDEPALMOILWITHOILBASEINWATER

15.15-15.30 OP020 Drs.IndingGusmayadi,M.Si.,Apt.

VARIATIONCONCENTRATIONOFCITRICACIDASACIDSOURCESONTHEPHYSICALPROPERTIESOFTHEPERICARPMANGOSTEEN(GarciniamangostanaL)EXTRACTSEFFERVESCENTTABLET

15.30-15.45 OP078 DyahHestiWardhani

IRONENCAPSULATIONUSINGGLUCOMANNANMODIFIEDWITHACETICACIDASAMATRIX

R.5MeetingRoom,2ndFloorModerator:Dr.AdeZuhrotunM.Si.,AptTime Kode Name Title14.00-14.15 OP031 LilisAdeliah EFFECTIVITYTESTOFETHANOLANDWATEREXTRACTS

ANDDROPSPREPAREATIONSOFREDDRAGONFRUITPEELASBORAXDETECTOR

14.15-14.30 OP033 NurAyuFitriani CAFFEINEVOLTAMMETRICSENSORBASEDONELECTROPOLYMERIZEDMOLECULARLYIMPRINTEDPOLY(GLUTAMICACID)ONCARBONPASTEELECTRODE

14.30-14.45 OP038 Dr.FauzanZeinM.,M.Si.,Apt.

PHARMACOPHOREMODELINGANDMOLECULARDOCKINGBASEDVIRTUALSCREENINGOFB-CELLLYMPOMA2(BCL2)INHIBITORFROMZINCNATURALPRODUCTDATABASE

14.45-15.00 OP042 DewiAstrianyM.Si.,Apt.

IN-SILICOANALYSISANDHOMOLOGYMODELINGOFNS1ANTIGENINDONESIAONDIAGNOSTICSENSITIVITYTESTOFDENGUE

15.00-15.15 OP052 FentiFatmawati,M.Si

ANALYSISOFLEADLEVELSONMASKARACOSMETICSFROMKIARACONDONGMARKETUSINGATOMABSORPTIONSPECTROPHOTOMETRYMETHOD


29

15.15-15.30 OP054 Dr.RimadaniPratiwi,M.Si.,Apt

COMPARISONOFBULKANDPRECIPITATIONPOLYMERIZATIONMETHODOFSYNTHESISMOLECULARIMPRINTEDSOLIDPHASEEXTRACTIONFORATENOLOLUSINGMETHACRYLICACID

15.30-15.45 OP083 Nellysuryani FORMULATIONOFPEELOFFMASKFROMLEMONPEELESSENTIALOILEXTRACT(CITRUSLIMONL.)ANDITSANTIOXIDANTSACTIVITYTEST

Day2Wednesday,23thOctober2018R.1BaleSawalaModerator:Dr.AliyaNurHasanahM.Si.,AptTime Kode Name Title13.30-13.45 OP060 RijantiRahaju

MaulaniTOTALFLAVONOIDSANDANTHOCYANINCONTENTOFPIGMENTEDRICE

13.45-14.00 OP025 DriyantiRahayu DESIGNOFINDICATORSTRIPSWITHCELLULOSEBASEFORDETECTIONOFPARACETAMOLINTRADITIONALMEDICINE

14.00-14.15 OP065 IsmiRahmawati SYNTHESISANDANTIBACTERIALACTIVITYTESTSOFCURCUMINANALOGUESUSINGBROMOFUNCTIONALGROUP

14.15-14.30 OP067 DrsTarwadi,MSc SynthesisandCharacterizationofLipopeptide-BasedTransfectionAgentasNon-ViralGeneDeliveryVehicleCandidate

14.30-14.45 OP069 DoniDermawan Host-GuestInteractionsofAlphaMangostinwith(α,β,andγ)−Cyclodextrins:SemiEmpiricalQuantumMechanicalMethodsofPM6andPM7

14.45-15.00 OP070 AyuShalihat SPECTROPHOTOMETRICDEVELOPMENTMETHODFORDETERMINATIONBORONCONTENTINFOODWITHPYRIDOXINECOMPLEXFORMATION

15.00-15.15 OP081 DewiSetyaningsih UV-VISSPECTROSCOPICMETHODFORSIMULTANEOUSESTIMATIONOFCURCUMINANDPIPERINEINDISSOLUTIONSAMPLESCONTAININGCURCUMALONGAANDPIPERNIGRUMSOLIDDISPERSION

R.2BaleRucitaModerator:FebrinaAmeliaSaputriM.Farm.,AptTime Kode Name Title13.30-13.45 OP045 NunukAries

Nurulita ANTIAGINGPOTENTIALOFMORINGAEXTRACTS


30

13.45-14.00 OP047 DESISAGITA CYCLODEXTRINEGLUKANOTRANSFERASEPRODUCTIONBYLOCALBACTERIAFROMCASSAVASOIL

14.00-14.15 OP053 Prof.Dr.NunukHarianiSoekamto,MS.

STIGMASTEROLOFETHYLACETATEEXTRACTFROMSTEMBARKOFMELOCHIAUMBELLATA(HOUTT)STAPFVAR.VISENIA(MALVACEAE)ANDDENGUEANTIVIRALACTIVITY

14.15-14.30 OP059 Dr.Ir.dadangSumardi,MP.

ISOFLAVONEANDANTHOCYANINCONTENTOFBLACKSOYBEANPROMISINGLINES

14.30-14.45 OP073 YoniAtma GELATINSASNATURALCOMPOUNDANDITSPROPORTION-RATIOWITHGLIPTINSTODIPEPTIDYLAMINOPEPTIDASEIV(DP-4)INHIBITION

14.45-15.00 OP075 NurulMahmudati PHENOLICCOMPOUNDSCONTENTANDANTIOXIDANTACTIVITYOFELEPHANTGINGER(ZINGIBEROFFICINALEROSCOE),REDGINGER(ZINGIBEROFFICINALEVARRUBRUM),ANDEMPRITGINGER(ZINGIBEROFFICINALEVARAMARUM)

15.00-15.15 OP079 ShirlyKumala ANTIBACTERIALACTIVITIESOFSECONDARYMETABOLITSINENDOFITFUNGIFROMTHEBARKOFBERINGIN(FicusbenjaminaLinn.)TREEINVITRO

R.3DRPM4thFloorModerator:Dr.NyiMekarSaptariniM.Si.,AptTime Kode Name Title13.30-13.45 OP021 SitiWanda

NurwantiINVITROANTIOXIDANTACTIVITYOFGARDENCROTON(Codiaeumvariegatum(L.)Rumph.ExA.Juss.)USINGDPPH(2,2-Diphenyl-1-Picrylhydrazyl)METHODANDPHYTOCHEMICALANALYSISUSINGGC-MS

13.45-14.00 OP022 andrianisusanty CYTOTOXICACTIVITYOFTAMPABADAKLEAVES(Voacangafoetida(Bl.)K.Schum)ONT47DBREASTCANCER

14.00-14.15 OP029 MADIHAH APPLICATIONOFCOMMONGREENBOTTLEFLY(Luciliasericata,Meigen1826)LARVAEEXTRACTFORINCISIONWOUNDTREATMENTSINRATS

14.15-14.30 OP032 Drs.Nohong,M.Si ANTIOXIDANTACTIVITYOFα–AMYRINANDβ-SITOSTEROLFROMBARKHEXANEXTRACTSOFRHIZOPHORAMUCRONATALAMK.

14.30-14.45 OP034 Rahmadevi ANTIOXIDANTOFLIMEPEEL(Citrusaurantifolia(Christm)Swingle)INEMULGEL

14.45-15.00 OP036 NoorRahmawati EXPLORATIONOFPOTENTIALBIOACTIVECOMPOUNDSOFENDOPHYTICMICROBIALCULTUREISOLATEDFROMGALLRUSTSENGON(FALCATARIAMOLUCCANA)Barneby&J.WGrimes


31

15.00-15.15 OP002 SandraMegantara BreastcanceractivityfromphytochemicalconstituentsofMorindacitrifolia:insilicoapproach

15.15-15.30 OP028 YelfiAnwar PRELIMINARYTESTOFUTILIZATIONOFAspergillusnigerINTHEPROCESSOFBIOTRANSFORMATIONOFGERANIOLANDITSIDENTIFICATIONWITHGASCHROMATOGRAPHY-MASSSPECTROMETRI(GC-MS)

R.4BaleRancage1,4thFloorModerator:Dr.IyanSopyanM.Si.,Apt

Time Code Title

13.30-13.45 OP023 RahmahElfiyani COMPARISONOFCARBOPOL934AND941ASTHICKENERSONDIFFUSIONRATEOFKETOCONAZOLEMICROEMULSION

13.45-14.00 OP030 YolaDesneraPutri FORMULATIONANDEVALUATIONOFCAFFEINEANTI-CELLULITEGELWITHADDITIONOFGLYCOLICACIDASENHANCER

14.00-14.15 OP039 DiahTriUtami,S.Si.,M.Sc.

POTENTIALETHANOLICEXTRACTOFPASAKBUMI(Eurycomalongifolia)NANOPARTICLESASANAGENTOFINCREASINGARTEMISININACTIVITYONPlasmodiumfalciparum

14.15-14.30 OP051 TaofikRusdiana,PhD.,Apt.

Pharmacokineticinteractionsbetweenwarfarinandjavanessturmeric(CurcumaxanthorrhizaRoxb)extractinrats

14.30-14.45 OP058 DENINOVIZA PREPARATIONANDCHARACTERIZATIONOFSOLIDDISPERSIONOFUSNICACID-GELUCIRE44/14WITHFREEZEDRYINGMETHOD

14.45-15.00 OP061 Buanasari,S.T.,M.T.

DEVELOPMENTANDCHARACTERIZATIONINMICROENCAPSULATIONOFPETAI(ParkiaspeciossaHassk.)LEAFEXTRACTS

15.00-15.15 OP066 AlasenSembiringMilala

EFFECTOFPEG400,PEG4000ANDPEG6000ASASUPPOSITORIABASEONPARASETAMOLDISSOLUTIONRATE

R.5MeetingRoom,2ndFloorModerator:Dr.RimadaniPratiwiM.Si.,Apt

Time Code Title

13.30-13.45 OP071 Dr.YatiSumiyati,M.Kes,Apt

ACTIVITYOFPORANGFLOURANDMORINGAEXTRACTTOBLOODGLUCOSEANDLIPIDLEVELSINALLOXANINDUCEDDIABETICMICE

13.45-14.00 OP077 PoppyDiahPalupi ANTIDIABETESEFFECTOFELECTROLYZEDREDUCEDWATERINTYPE2DIABETESMELLITUSPATIENTS

14.00-14.15 OP080 AgusSulaeman INFLAMMATIONSTATUSPROFILEINYOUNGADULTMENOBESE&NONOBESESTUDYOFHIGHSENSITIVITYC-REACTIVEPROTEIN(hsCRP)SERUMLEVEL


32

14.15-14.30 OP026 SitiWakhidatunSuciyati,M.Si,Apt.

REDGINGER(ZingiberofficinaleRoscoevar.SuntiVal)EXTRACTSINHIBITSIĸBαPHOSPHORYLATIONINTHENFĸBINFLAMMATORYPATHWAYOFNEONATALRATCARDIOMYOCYTES

14.30-14.45 OP076 WahyuWidowati ANTIOXIDANTandANTIAGINGofNepheliumlappaceumPEELSEXTRACTandITSCOMPOUNDS

14.45-15.00 OP068 SabarPambudi ComputationalantigenicepitopepredictionofclinicalIndonesianDenguevirusNS1protein

15.00-15.15 OP082 vinafatimahotuh PREPARATIONANDEVALUATIONOFTHEEFFERVESCENTGRANULESFROMBREADFRUIT(Artocarpusaltilis(Parkinson.)Fosberg)LEAFETHANOLEXTRACT

TimeScheduleforPosterSession

Schedule Time PosterCode LocationDay1Tuesday,October23,201

07.30-17.00 PP01-PP68 BaleSawalaLobby

Day2Wednesday,October24,2018

07.30-17.30 PP70-PP128 BaleSawalaLobby


33

KeynoteSpeaker

Prof.dr.NilaDjuwitaF.Moeloek,Sp.MisaMinisterofHealthRepublicofIndonesia.Shewasbornat Jakarta on April11th 1949 and an expert in eye tumor at Faculty of Medicine, UniversitasIndonesia. She is a special deputy of the President Republic of Indonesia forMDGs (MilleniumDevelopmentGoals)on2009-2014.


34

KeynotespeakerII

HidetoshiArimaisProfessorofGraduateSchoolofPharmaceuticalSciences,KumamotoUniversity,Japan. HereceivedaPh.D. in1991fromKumamotoUniversity in Japan.From1991to1993,heworkedatEisaiCo.,Ltd.inJapan.From1993-1998,heworkedatTokyoUniversityofPharmacyandLifeSciencesas researchassociate. In1998,hemoved toFacultyofPharmaceutical Sciences inKumamotoUniversityinJapan,andthenwaspromotedtoassociateprofessorin2001.In2007,hewaspromotedtoProfessorinGraduateSchoolofPharmaceuticalSciences,KumamotoUniversityinJapan. Hisresearchinterest isdesignandevaluationof integrateddrugdeliverysystem(DDS)basedoncyclodextrins.Inaddition,hestartedtoresearchthepotentialuseofsacran,asupergiantcyanobacterialpolysaccharide,asnaturaldrugsandDDScarriers.Over150researchpapersand10patentshavebeenpublishedsince1986.FurthergrowthoftheDDSresearchisexpected.

POTENTIALUSEOFCYCLODEXTRINSASMULTI-FUNCTIONALDRUGCARRIERSANDACTIVEPHARMACEUTICALINGREDIENTS

HidetoshiArima

GraduateSchoolofPharmaceuticalSciences,KumamotoUniversity,Kumamoto862-0973,Japan

E-mail:[email protected]

ABSTRACTCyclodextrins(CyDs)arewidelyusedinavarietyoffieldssuchasfoods,beverages,dailynecessities(deodorants),cosmetic,neutraceuticalandpharmaceuticalfields.Inparticular,thewidespreaduseofCyDsaspharmaceuticalexcipientsiswellknowninthepharmaceuticalfield,becauseCyDscanimprove the solubility, dissolution rate, and bioavailability of the lipophilic drugs aswell as thestabilityoflabiledrugs.Recently,CyDsareattractingattentionasdrugcarriers.Forexample,werevealedthatfolate-appendedβ-CyDcouldbeasapromisingtumortargetingcarrierforantitumordrugs[1].Inaddition,weclarifiedthatpolypseudorotaxane(PPRX)hydrogelsconsistingofCyDsandpolyethylene glycol are useful as safe and promising stabilizing formulations for antibody-baseddrugs [2]. Moreover, we newly designed the self-assembly Pegylation retaining activity (SPRA)technologyviaahost-guestinteractionsurpassingconventionalpegylationmethodsofproteins[3].Also,wedemonstratedthepotentialoffolate-PEG-appendeddendrimer(G4)/a-CyDconjugatesasatumor-selectivesiRNAcarrier[4]. Thus,CyDscouldbepromisingasdrugcarriershavingmulti-functional and biocompatible characteristics for low-molecular weight drugs, protein drugs andoligonucleotidedrugs.Meanwhile,CyDscouldbeusefulforactivepharmaceuticalingredients(APIs)


35

againstvariousdiseasessuchasNiemannPickDiseaseTypeC,solidcancers[5]andamyloidosis.Inmypresentation,IintroducethepotentialsofCyDsasmulti-functionaldrugcarriersandAPIs.Keywords: cyclodextrins, tumor targeting, polypseudorotaxane, supramolecules, activepharmaceuticalingredients


36

KeynotespeakerIII

VeyselKayserisanAssociateProfessorintheFacultyofPharmacyattheUniversityofSydney.HereceivedhisPh.D.fromtheUniversityofLeeds(UK)thenperformedpost-doctoralresearchattheMax-PlanckInstitute(Germany)andatMIT(US).BeforejoiningtheUniversityofSydneyhewasaseniorscientistatMIT.Healsoconsultsforpharmaandbiotechcompaniesandlawfirms.AssociateProfessorKayser’sresearchfocusesonthedevelopmentofvaccinesandbiologicalproducts.

NEWMETHODSFORTHEDEVELOPMENTANDSTUDYOFDEGRADATIONOFBIOLOGICSANDVACCINES:THERAPEUTICMONOCLONALANTIBODIESANDINFLUENZAVACCINES

VeyselKayser

TheUniversityofSydney,FacultyofPharmacy,Sydney,NSW2006,Australia


ABSTRACTBiologics and vaccines are now an integral part of the modern healthcare system. However,developing them is complicated, expensive and time consuming.Manufacturing and processingstepsgreatlyaffecttheirstabilityandmostofthemsufferfromformulationstabilityissuesduetotheir complex nature. For example, functional and immunological repercussions of degradationproducts of therapeuticmonoclonal antibodies (mAbs) are often amajor concern. Degradationoccurs mainly via protein aggregation, which are known to be immunogenic; therefore,understandingitsmechanismandaccuratequantificationofaggregatesisimportant.Similarly,theformulationstabilityofseasonalfluvaccinesisoftenproblematic.Theaimisproducingavaccinethatishighlyimmunogenic;however,ithasalowlevelofreactogenicity.Surfactantsareusedto‘split’thevirusesandthis‘splitting’processaffectstheformulationstabilityandpotencyoffluvaccinessignificantly.Hence,optimisingmanufacturingconditionsandestimatingthesplit-ratioandleftoversurfactantineachstepisofutmostimportanceforrapidpreparationoffluvaccines.Iwillpresentseveralnewapproachesthatwehavedevelopedoverthepastfewyears–employingspectroscopy,chromatographyandmicroscopy,tostudyproteinaggregationintheformulationsofmAbs,toquantitativelyestimatethesplit-ratiooffluvirusfollowingsurfactanttreatment,andtodetermineleftoversurfactantininfluenzavaccines.Thisstudyformsthebasisofanin-situmethodtoquantifysplitvirusesandleftoversurfactantduringvaccinemanufacturing,andwillfacilitatetherapiddevelopmentofthefluvaccineinamorecontrolledmanner.Itwillalsobebeneficialforthedevelopmentofotherbiologicsincludingbiosimilarsofmonoclonalantibodiesforthedetectionofproteinaggregation.Keywords:Degradation,vaccines,stability,monoclonalantibodies,proteinaggregation


37

InvitedSpeakerI

Prof.Dr.Dato’IbrahimJantanisacademicstaffofFacultyofPharmacyUniversitiKebangsaanMalaysia.HeisexpertiseinMedicinalandNaturalProductChemistry.HeisalsoPresidentofMalaysiaNaturalProduct(MNPS).

INTEGRATEDAPPROACHESINNATURALPRODUCTSRESEARCHFORDISCOVERYOFNEWIMMUNOMODULATORYAGENTS

IbrahimJantan

DrugsandHerbalResearchCentre,FacultyofPharmacy,UniversitiKebangsaanMalaysia,JalanRajaMudaAbdulAziz,50300KualaLumpur,Malaysia


ABSTRACTImmunodrugsincludeorganicsynthetics,biologicalagentssuchascytokinesandantibodiesactingonsingletargetsorpathways,havebeenusedtotreatimmune-relateddiseasesbutwithlimitedsuccess.Mostoftheimmunomodulatorsinclinicalusearethecytotoxicdrugswhichpossessseriousside effects. There is a growing interest to use herbalmedicines asmulti-component agents tomodulate the complex immune system in the prevention of infections rather than treating theimmune-relateddiseases.Manytherapeuticeffectsofplantextractshavebeensuggestedtobeduetotheirwidearrayofimmunomodulatoryeffectsandinfluenceontheimmunesystemofthehumanbody. Most immunological studies on plants were based on crude extracts and the bioactivecompoundsresponsibleforthebioactivitieshavenotbeenwellidentified.Thereby,itisnecessarytoconductdetailedsystematic studies toevaluate theefficacyandmechanismsofactionof theplantsusingtheirstandardizedextractsandbioactiveisolatesonspecificaspectsoftheimmunesystem,includingbothadaptiveandinnatearmsoftheimmuneresponse.Phytochemicalssuchasflavonoids, polysaccharides, lactones, alkaloids, diterpenoids and glycosides, present in severalplants,havebeenreportedtoberesponsiblefortheplantsimmunomodulatingproperties.Thusthe search for plant-based natural products as new leads for development of potent and safeimmunosuppressantandimmunostimulantagentsisgainingmomentum.Plant-derivedcompoundssuch as curcumin, resveratrol, epigallocatechol- 3-gallate, quercetin, colchicine, capsaicin,andrographolide, and genistein have exhibited potent effects on cellular and humoral immunefunctionsinpre-clinical investigationsandsomehaveshownclinicalpotential. Severalmedicinalplantswhichareusedintraditionalmedicinetotreatmicrobialandviralinfections,fever,allergyandvariousinflammatoryconditionswereselectedtostudytheireffectsondifferentcomponentsoftheimmunesystem:inhumoralandcellularimmunefunctionswhichincludespecificactionsonimmunecells,effectormechanisms,inhibitionofnitricoxide(NO)andreactiveoxygenspecies(ROS)


38

production,secretionofinflammatorycytokines,lymphocytesproliferation,signalingpathwaysinmacrophage cells, and phagocytic activities. The standardized plant extracts and their markercompoundswereevaluatedfortheirinvitroeffectsonchemotaxis,β2integrin(CD18)expression,phagocytosis, ROS and NO production of human phagocytes, lymphocytes proliferation andcytokinesreleasefromperipheralbloodofmononuclearcells(PBMCs).Theeffectsofthesampleson lipopolysaccharide (LPS)-induced nuclear factor-kappa B (NFкB), mitogen activated proteinkinases (MAPKs) and protein kinase B (Akt) activation in U937 human macrophages were alsoinvestigated. The samples were also assessed for their in vivo modulatory effects on thehumoralandcellularimmunefunctionsbothininnateandadaptiveimmuneresponsesinratsandmice.Keywords:Naturalproducts,immunomodulatory,Immunodrugs


39

InvitedSpeakerII

Anis Yohana Chaerunisaa is Assistant Professor in Department of Pharmaceuticsand Pharmaceutical Technology at Faculty of Pharmacy, Universitas Padjadjaran, BandungIndonesia. Shehasbeenheadofundergraduateprogramherfacultysince2015andhasbeenalecturersince1998.SheobtainedabachelordegreeinPharmacyfromUniversitasPadjadjaran,aMasterofScienceinPharmaceuticalTechnologyfromInstitutTeknologiBandung,andfinishedherPhD in College of Pharmacy, Freie University of Berlin, Germany. Her main fields of interest isPharmaceutical Technology and her recent work in this area includes solid dosage forms andmodifiedrelease,coatinganddevelopmentofpolymerandotherpharmaceuticalexcipients.Sheistheauthorofmanyscientificpublicationsinthefieldofpharmacy.

DEVELOPMENTOFPHARMACEUTICALEXCIPIENTSFORORALSOLIDDOSAGEFORMSFROMLOCALSOURCES

AnisYohanaChaerunisaa

DeptofPharmaceuticsandPharmaceuticaltechnology,FacultyofPharmacy,UniversitasPadjadjaran


ABSTRACT

Excipients play an important role in formulating a dosage form for providing essentialmanufacturingtechnologyfunctions.Informulation,itactasfillers,diluents,antiadherents,binders,coatings,flavours,colours,lubricants,glidants,preservatives,sorbentsorsweeteners.Thesourceofthesematerialscouldbenaturallyoriginatedorfromsyntheticproduction.Naturalsourceoffermore advantages since they are chemically inert, nontoxic, less expensive, biodegradable andabundantlyavailable.Indonesiaisrecognizedasbeingtherichestcountryintermofbiodiversity,naturalsourceandmarineorganismsaswell.Theseresources isamassivesuppliesforfoodandpharmaceuticalpurposes.Starch,Microcrystalinecelluloseandcarrageenanarethoseamongtheexcipient with high need in formulation either as filler, matrix polymer, binder or disintegrant.Explorationofcornstarchastabletfillerwasfirstdevelopedfromgeneticarchitectureoflocalcorntoobtainhighcarbohydratecontentmaize.Theisolationandcharacterizationwereoptimizedtoprovide pharmaceutical gradequality. Further study on the employment of isolated corn starcheither as filler, disintegrant and binder had been carried out. Carrageenan, verywell known asexcipientinfoodandpharmaceuticalindustrywasaslobeendevelopedfromlocalseaweeds.Mostcarrageenan is extracted from Kappaphycus alvarezii or red seaweed. Indonesia provides an


40

unlimitedandoptimalenvironmentforcultivatingthisseaweed.Isolationandformulationstudyofcarrageenan as matrix tablet has been conducted using different type of drug as ActivePharmaceuticalIngredients.Specificexcipientsbestsuitedforaparticulardosageform;forexampleMicrocrystalinecellulose(MCC)asfillerfordirectcompression,offerhigheconomicvalueintabletmanufacturing. This special property offered by MCC originated from Ramie as alternative ofhardwood,thealmostextinctnaturalsourceofMCC.Developmentofexcipientsaslikeotheractivepharmaceuticalingredientsneedtobestabilizedandstandardized;alongwiththesafetyevaluationparametersoftheexcipients.Studyontoxicityofthesematerialshasbeenconductedandrevealedthattheyaresafetobeused.Keywords:Carrageenan,Cornstarch,MCC,pharmaceuticalexcipients


41

InvitedspeakerIII

Prof. Dr. Mohd Cairul Iqbal Mohd Amin is academic staff of Faculty of Pharmacy UniversitiKebangsaanMalaysia.HeisexpertiseinNovelDrugDelivery,DosageFormDesign,Hydrogel,andPharmaceutical Science. He is also President of The Malaysia Local Chapter of The ControlledReleaseSocietyInc.(MyCRS).BACTERIALCELLULOSE-BASEDHYDROGELASNEWCELLCARRIERFORTHETREATMENTOFFULL-

THICKNESSWOUNDINJURY

MohdCairulIqbalMohdAmin

FacultyofPharmacyUniversitiKebangsaanMalaysiaE-mail:[email protected]

Bacterial cellulose (BC)/acrylic acid (AA) hydrogel has been previously investigated as a wounddressing for partial-thickness burn wound and shown to accelerate healing. It is a promisingbiomaterialcandidateasacellcarrierbecauseitbearssomeresemblancetothethree-dimensionalarchitectureofextracellularmatrixinnativetissues.Thisstudyextendsitsuseasacellcarrier,inaddition to a wound dressing, for delivering human epidermal keratinocytes (EK) and dermalfibroblasts(DF),whichcouldleadtoimmediatetreatmentoffull-thicknessskinlesions.ThehydrogelwaspreparedusingBC(60%),crosslinkedwithAA(40%)viaelectronbeamirradiation(35kGy).In-vitrostudiesdemonstratedthatBC/AAhydrogelwasbiocompatible,hadexcellentcellattachmentwithin4hours,maintained cell viabilitywith limitedmigrationandallowed cell transfer. In vivowound closure, histological, immunohistochemistry and transmission electron microscopyevaluation revealed that hydrogel alone (HA) and hydrogel with cells (HC) accelerated woundhealingcompared to theuntreatedcontrols.GrossappearanceandMasson’s trichromestainingshowedresultsinfavourofHCcomparedwithHA.ThisstudysuggeststhepotentialapplicationofBC/AAhydrogelwithdualfunction,viz.asacellcarrierandwounddressingtopromotefull-thicknesswoundhealing.Keywords:BacterialCellulose,hydrogel,woundhealing


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InvitedspeakerIV

Prof. Dr. Yahdiana Harahap,M.S., Apt. is a professor on Chemical Pharmacy Analysis especiallyBioanalysis and DNA-Adduct Analysis. Based on the field of science, currently many developedmethodsofanalysisinbiologicalmatrices,especiallyrelatedtobioequivalencestudiesforqualityassuranceofdrugs,especiallygenericdrugs.ShehasbeenanExpertTeamattheNationalAgencyofDrugandFoodControlsince2008,KANISO17025LaboratoryAssessorsince2010,amemberofthe IndonesianPharmacistBondExpertCouncil,ExpertWitness in theNationalNarcoticsBoard,reviewer in several international journals, as Vice President of the Asian Federation ofPharmaceutical Science, and as Chairman of the Pharmaceutical Division at the Institute forAccreditationofIndependentHigherHealthEducation(LAMPT-Kes).

BIOANALYSIS:CONNECTINGPHARMACEUTICALSCIENCEANDCLINICALPHARMACY

YahdianaHarahap

FacultyofPharmacyUniversitasIndonesia,Depok,IndonesiaE-mail:[email protected]

ABSTRACT

The pharmaceutical sciences combine a broad range of scientific disciplines including analyticalchemistry. Bioanalysis is sub-discipline of analytical chemistry covering the quantitativemeasurement of xenobiotic (drugs and their metabolites, and biological molecules) and biotics(macromolecules,proteins,DNA,largemoleculedrugs,metabolites)inbiologicalsystems.Thefocusofbioanalysisinthepharmaceuticalindustryistoprovideaquantitativemeasureoftheactivedrugand/oritsmetabolitesforthepurposeofpharmacokinetics,toxicokinetics,bioequivalencestudies,and exposure-respons (pharmacokinetics / pharmacodynamics studies). While in the clinicalpharmacythereistherapeuticdrugmonitoringwhichistoensurethatthetherapygivenalreadyappropriate that increase the quality life of the patient through bioanalysis (determinationofanalyteandmetabolitelevelinbiologicalmatrix).Basedonthat,bioanalysiscanmakethebridgebetween pharmaceutical sciences with clinical pharmacy. Some researches had been done incollaborationwithhospitalfortherapeuticdrugmonitoringsuchastuberculoseandcancertherapy.Keywords:bioanalysis,clinicalpharmacy,pharmaceuticalsciences,therapeuticdrugmonitoring


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InvitedspeakerV

Assoc.Prof.NurulAsmaAbdullah,Ph.D.isacademicstaffofSchoolofHealthSciencesatUniversitiSainsMalaysia.ShereceivedaPh.D.in2008fromUniversitiSainsMalaysia.HerfieldsofspecializationareMolecularImmunology,RegenerativeMedicine,andVaccinology.Lignosusrhinocerotis(Cooke)RYVARDEN(TIGERMILKMUSHROOM):ANALTERNATIVEFORTHE

MANAGEMENTOFALLERGICAIRWAYINFLAMMATIONNurulAsmaAbdullah1*,JohnathanMalagobadan1,SitiAminahMuhamad1,SitiNurshazwaniMuhamadSayuti1,BushraSolehahMohdRosdan1,GanSiewHua2,3,WanEzumiMohdFuad1,SabreenaSafuan1,FaezahtulArbaeyahHussein2,HabibahAWahab4,MohamadNurulAzmi

MohamadTaib5andJohnsonStanslas6

1SchoolofHealthSciences,UniversitiSainsMalaysia,16150KubangKerian,Kelantan,Malaysia2SchoolofMedicalSciences,UniversitiSainsMalaysia,16150KubangKerian,Kelantan,Malaysia3SchoolofPharmacy,MonashUniversityMalaysia,47500BandarSunway,Selangor,Malaysia

4SchoolofPharmaceuticalSciences,UniversitiSainsMalaysia,11800Penang,Malaysia5SchoolofChemicalSciences,UniversitiSainsMalaysia,11800Penang,Malaysia

6FacultyofMedicineandHealthSciences,UniversitiPutraMalaysia,43400Serdang,Selangor,Malaysia

*E-mail:[email protected]

ABSTRACTThe Lignosus rhinocerotis (Cooke) Ryvarden (Tiger Milk mushroom), has long history of use asnatural remedies forvariousdiseasesby the localand indigenouscommunities inMalaysia.Thisfungusisclassifiedasmedicinalmushroom.L.rhinocerotishasbeenscientificallyinvestigatedforvarious therapeutic purposes including anti-inflammatory, anti-coagulant, anti-microbial, anti-obesity,anti-cancerandantioxidantproperties;howevertodate,itsefficacyonasthmaislimited.Asthmaisachronicinflammatoryairwaydiseasethataffectsaround300millionpeopleworldwide.Thediseaseischaracterisedbyvariabledegreesofchronicinflammationandairwayremodelling.Currentasthmamedicationsaremainlybasedonsteroidssuchasshortactingβ-agonists,inhaledcorticosteroids,andlongactingβ-agonists.Unfortunately,prolongeduseofthesedrugscancauselocalandsystemicsideeffects.Thus,asaferalternativeforthemanagementofasthmaisneeded.We investigated the anti-asthmatic effects of L. rhinocerotis extract (LRE) in acute and chronicmodels.Animalsweresensitizedwithamixtureofovalbuminandaluminiumhydroxidefollowedby


44

challengestotheallergensseveraltimes(accordingtothemodels).TreatmentsusingLREat125,250and500mg/kgweregiven.WedemonstratedthatLREtreatmentsignificantlyamelioratedtheincrease intotal immunoglobulinE (IgE) inserumand interleukin-4(IL-4), IL-5and IL-13 levels inbronchoalveolar lavage (BAL) and also effectively suppressed eosinophil numbers in BAL whileattenuatingtheleukocytesinfiltrationandgobletcellhyperplasiaintheperibronchialregionsofthelungstissuesaswellasreducethesmoothmusclecellhypertrophy. Inaddition,LREsignificantlyaltered regulatory T cell population as well as TGF-β and Activin A expressions in the animals.Transcriptome analysis revealed a total of 21 asthma-related genes were successfullydownregulatedwith LRE treatment. Our findings suggest the potential of L. rhinocerotis to bedevelopedasanalternativeforthemanagementofallergicairwayinflammation.Keywords:Allergicairwayinflammation;asthma;Lignosusrhinocerotis;TigerMilkmushroom


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InvitedspeakerVI

Prof. Muchtaridi is a full professor in Department of Pharmaceutical Analysis and MedicinalChemistryofFacultyofPharmacy,UniversitasPadjadjaran, Indonesia.HeobtainedhisPhD fromSchoolofPharmaceuticalSciences,UniversitiSainsMalaysiain2013underthesupervisionofProf.Dr.HabibahA.Wahab.Hismainresearch interest isdrugdiscoveryofnaturalproductsandthatincludesbio-guideassayisolationcombaininginsilicoi.e.virtualscreeninganddesignagainstanti-breastcancerandanti-influenza.Hiscurrentresearchispotentialactivityofsomenaturalproductscompoundsasanti-breastcancerandneuraminidaseinhibitorsbasedonstructureandligandbasedandinvitrotest.

3DSTRUCTURE-LIGANDBASED,ADMEPREDICTION,ANDMOLECULARDYNAMICOFα-MANGOSTINANDITSDERIVATIVESAGAINSTESTROGENRECEPTORα

DoniDermawan1,MuhammadYusuf2,SharonOBryant3andMuchtaridiMuchtaridi1,*

1DepartmentofPharmaceuticalAnalysisandMedicinalChemistry,FacultyofPharmacy,

UniversitasPadjadjaran;Jln.RayaBandung-SumedangKM.21,Jatinangor45363,WestJava,Indonesia;

2DepartmentofChemistry,FacultyofMathematicsandNaturalSciences,UniversitasPadjadjaran,Jln.RayaBandung-SumedangKM.21,Jatinangor45363,WestJava,Indonesia;

3Inte:LigandGmbH,Mariahilferstrasse74B/11,A-1070Vienna,Austria*E-mail:[email protected];

ABSTRACT

α-mangostin is themain active compound ofGarciniamangostana pericarp, which inhibits theproliferation of the MCF-7 cell line with an IC50 10 µM. Molecular docking simulation and 3Dstructure-basedpharmacophoremodelswereemployedtoidentifythemolecularinteractionsofα-mangostinanditsderivativesagainstestrogenreceptorα(ERα).Theresultsshowedthatthebindingenergyofα-mangostinanditsbestderivative(AMD10)were−9.05kcal/moland−11.89kcal/mol,respectively. These compounds also interacted with Thr347, Asp351, Met388, Met528, Ile424,Arg394,andGlu353.Thepharmacophore-fitscoresofα-mangostinandAMD10were83.06%and86.46%,respectively. Inaddition,theabsorption,distribution,metabolismandexcretion (ADME)propertieswerepredicted.BasedontheresultofmoleculardynamicsusingMM-PBSAcalculationmethod, it is known thathERα-AMD10systemhasΔGTOTAL=−52,57kcal/mol compared tohERα-estradiolsystemwithΔGTOTAL=−40,86kcal/mol.Theseresultsshowedthatα-mangostinandAMD10arepromisingcandidatesofnovelanti-breast-canceragentswithantagonisticactivitytoERα.Keywords:α-mangostin,estrogenreceptoralpha,moleculardockingandpharmacophore


46

InvitedspeakerVII

Dr.NurKusairaBintiKhairulIkramisacademicstaffofInstituteofBiologicalSciences,FacultyofScienceatUniversityofMalaya.ShereceivedaPh.D.(Biotechnology)in2017fromUniversityofCopenhagen,Denmark.HerfieldsofspecializationareMetabolicPathways(Plantbiochemistry)andTransgenicPlant(Plantbiotechnology-Geneticandmetabolicengineeringandrelatedtransgenicresearch).

ANEWPLANT-BASEDDRUGPRODUCTIONPLATFORM

NurKusairaBintiKhairulIkram

InstituteofBiologicalSciences,FacultyofScienceatUniversityofMalayaE-mail:[email protected]

ABSTRACT

Physcomitrellapatensisanon-vascularplantthathasbeenwellestablishedasamodelorganismtobeused inbasic researchand in appliedbiotechnology. Thegenome is fully sequencedand thehaploidlifecycleandefficienthomologousrecombinationmakesP.patensanattractiveindustrialproductionsystemcomparedtootherplanthosts.Additionally,anoveltransformationtechnologyinvolving in vivoassemblyofmultipleDNA fragments inP.patenshasbeenestablished, furtherincreasingthepotentialasaphotosyntheticchassisforsyntheticbiology.Withthis,P.patenshasbeen chosen as the candidate organism for production of terpenoid-based drugs derived fromplants,withaprimaryfocusonthesesquiterpenelactones,artemisinin(antimalarialdrug).ThefivegenesinvolvedinartemisininbiosynthesiswereengineeredintothemossPhyscomitrellapatensviadirect in vivo assembly of multiple DNA fragments. Our study shows that P. patens can be asustainable and efficient production platform of artemisinin that without further modificationsallow for industrial-scale production. A stable supply of artemisinin will lower the price ofartemisinin-basedtreatments,hencebecomemoreaffordabletothe lower incomecommunitiesmostaffectedbymalaria;animportantsteptowardcontainmentofthisdeadlydiseasethreateningmillionseveryyear.Keywords:Physcomitrellapatens,DNA,productionplatform


47

InvitedspeakerVIII

SubramaniParasuraman,M.Pharm.,Ph.D.isacademicstaffofFacultyofPharmacyatAIMSTUniversity,Malaysia.HeisinterestedinPharmacologyandToxicology.HeisalsoEditorialBoardonJournalofYoungPharmacists.BIOCHEMICALANDPATHOLOGICALALTERATIONSINDUCEDBYELECTROMAGNETICRADIATION

INSPRAGUE-DAWLEYRATS

SParasuraman1,2,*,EngYuhXin1

1FacultyofPharmacy,AIMSTUniversity,08100Bedong,Malaysia.

2Editor-in-Chief,JournalofYoungPharmacist.*E-mail:[email protected]

ABSTRACT

Electromagnetic radiation (EMR) is playing important role in daily life to fulfill the educational,communicationandhealthcareneedsofhumans.Wi-FiradiationisatypeofEMRandwidelyusedto provide internet connectivity. A prolonged exposure of these radiationsmay alter the basalphysiologicalandbiochemicalfunctionsofmammalians.TheSpragueDawleyrat,whichexposedtoWi-Firadiationfor30daysshowedincreasedlevelsoffreeradicalsandalterationsinbiochemicalparameter.EMRexposedanimalsshowedsignificantincreaseinbodyweight(P<0.05);reducedfoodintake(P<0.05);decreasedlocomotormotion(P<0.05)andmuscularstrength(P<0.05)attheendofthestudywhencomparewiththatofcontrol.Inbiochemicalanalysis,elevatedlevelsofAST,ALT,creatinineandureaandreducedlevelsofglucosewereobservedinEMRexposedgroup.Antioxidantactivitysuchasreducedglutathioneandcatalasewasdecreasedcomparetonormalcontrolgroup.In histopathological analysis of brain of EMR exposed animals exhibited foci of edematousthickening,hyperemiccongestion,occasionalareasofdegenerativechanges, reactivegliosisandincreasedintheneuronalpopulation.ChronicexposureofEMRradiationaffectstheregularweightbodyweightgain;altersbehaviouralandbiochemicalparameters,decreasestheantioxidantlevelsandaffectthehistologyofbraincells.Keywords:Electromagneticradiation,SpragueDawleyrat,Antioxidant,histopathology


48

OralPresentations[OP001]

SYNTHESISOFMOLECULARIMPRINTEDPOLYMERFORATENOLOLRECOGNITIONUSINGITACONICACIDASFUNCTIONALMONOMER

AliyaNurHasanah*,SandraMegantara,RimadaniPratiwi,KhanifahPuspanegara

PharmaceuticalAnalysisandMedicinalChemistryDepartment,FacultyofPharmacy,UniversitasPadjadjaran,Jl.RayaBandungSumedangKm21,5Jatinangor,45363


ABSTRACT

Atenololisacardioselectiveβ-blockerthatusedforthetreatmentofhypertensionforalongperiod, but nowadays atenolol has big potential to be used as doping in several sports.Therefore,anefficientandselectiveseparationmethodisrequiredtodetectandmonitorthelevelofatenololinthebody.MolecularimprintedSolidPhaseExtractionisamethodthathasbeen developed as a specific recognition sample preparation method. This study aims todeterminetheadsorptionprofileandthephysicalcharacteristicsofthemolecularlyimprintedsorbentforatenololthatusesitaconicacidsasafunctionalmonomerincombinationporogenofmethanolandacetonitrile(1:1)usingprecipitationpolymerization.Themethodologythatused in this study includes determination of association constant of template- monomercomplex, synthesis of sorbent MI-SPE atenolol, extraction of atenolol template from thepolymer,evaluationoftheadsorptionabilityandcapacityofsorbentMI-SPE,determinationofsorbent’s selectivity, and physical characterization using Fourier Transform Infra-Red andScanningElectronMicroscope.Theresultshowedthatitaconicacidincombinationporogenofmethanolandacetonitrile (1:1)couldproducegoodadsorptioncharacter toatenolol in thesynthesis of sorbentMI-SPE atenolol by precipitation polymerization.MI-SPE sorbent giveshighporositythantheNIP;itisindicatingthattheatenololrecognitioncavityisformed.Futurestudy is needed to develop thisMI-SPE atenolol to the real biologic samples to know thesorbent’sperformanceontherealsamples.

Keywords:atenolol,molecularimprintedsolidphaseextraction,itaconicacid,combinationporogen,sorbent’scharacterization


49


PARTICLEDESIGNOFPARACETAMOLBYSPHERICALCRYSTALLISATIONTECHNIQUE

Indra*,ImaKrismayanti,RikaYulianti

ProgramStudiFarmasiSTIKesBaktiTunasHusadaTasikmalaya,46115


ABSTRACT

Paracetamolisananalgesic-antipyreticdrug,exhibitspoorwatersolubilityandflowproperties.A spherical crystallisation of paracetamol was prepared as part of efforts to improvemicromeriticproperties.Neutralizationtechniquewiththeadditionofβ-cyclodextrinadaptedspherical crystallisation. Crystallization medium was used for spherical crystallisation ofparacetamolconsistedof0.5Nsodiumhydroxide;0.2Nhydrochloricacidcontaining0.1%β-cyclodextrinand chloroform (bridging liquid) in a ratioof50:125:30 (v/v), respectively. Thespherical crystallisation was characterized by powder X-Ray diffraction (PXRD), DifferentialScanning Calorimetry (DSC), Fourier Transforms Infrared Spectrophotometry and ScanningElectronMicroscope(SEM).Micromeriticbehaviourstudieswerecarriedout for flowability,theangleofrepose,bulkdensity,tappeddensity,truedensity,Carr’sindex,Hausnerratio,andcompressionpercentage.Determinationof thecontentofparacetamolanddissolutionalsowas performed. XRD, DSC and FTIR spectrophotometry outcome showed no chemicalalteration of paracetamol during the spherical crystallisation process, and SEM outcomeshowedthatthecrystalshapedbecomesspherical.Furtherevaluationrevealedthatsphericalcrystallisation improved micromeritics properties compared with pure paracetamol.Determination of the content of paracetamol showed the percentage of 98.62%. Thedissolutionofthesphericalcrystallisationwasenhancedcomparedtopureparacetamol.

Keywords:Paracetamol,SphericalCrystallisation,Dissolution


50


DURIOZIBETHINUS(DURIAN)FRUITSWASTEMEDIATEDGREENSYNTHESISOFSILVER

NANOPARTICLES:AGREENAPPROACH

VeerasamyRavichandran1*,SamuggamSumitha2,SethuVasanthi3,SureshVChinni2,SubashCBGopinath4,PeriyasamyAnbu5

1FacultyofPharmacy,AIMSTUniversity,Semeling,Bedong,Kedah,Malaysia2FacultyofAppliedScience,AIMSTUniversity,Semeling,Bedong,Kedah,Malaysia.

3FacultyofEngineering,TheUniversityofNottingham,Semenyih,Selangor,Malaysia.4SchoolofBioprocessEngineering,UniversityMalaysiaPerlis,Malaysia.

5DepartmentofBiologicalEngineering,InhaUniversity,Incheon402-751,RepublicofKorea.

*E-mail:[email protected],[email protected]

ABSTRACT

Nowadays,environmentalpollutionisamatterofconcern;everybodyistryingtomakematerialsthatshouldbeecofriendly.Numerousapproaches,usingchemicalsorelectrochemical,areusedinthepreparationofnanoparticles.Generally,manyof thesestrategiesarehavingdifficulty in thepurification stage since the used chemicals or the by-products formed are hazardous and highenergy required for the preparation. The main aim of the present work was to develop greenapproach for the synthesisof silvernanoparticles (DRAgNPs)usingDurio zibethinuswaste (rind)aqueousextractanddeterminationof itsantibacterial, cytotoxiceffectagainstbrineshrimpandphotocatalyticactivity.Aqueousextractofdurian rindwasused to reducesilvernitrate tosilvernanoparticles.ThevariousreactionparameterswereoptimizedandDRAgNPswascharacterizedforsize, shape and nature. Surface plasmon resonance confirmed the formation of DRAgNP’s withmaximumabsorbanceatλmaxof418nm.SEMandTEMimagesrevealedofthemorphologyoftheDRAgNPsweresphericalwithsizerangeof20nmand60nm.AFMimagesconfirmedtheaverageparticlessizeofDRAgNPswastobe55nm.Thestabilityofthenanoparticleswasalsoconfirmedbythezetapotentialwhichwasfoundtobe-15.82mV.XRDandEDXanalysisconfirmedthenatureand presence of Ag. DRAgNPs showed considerable antibacterial activity against Salmonellatyphimurium, Escherichia coli, Salmonella typhi, Staphylococcus aureus, Staphylococcushaemolyticusand Bacillus subtilis,exhibitedbetter cytotoxicity againstbrine shrimp (LC50 =2.55mg/mL) and shownbetter photocatalytic activity againstmethylene blue. Based on the presentstudy,itcanbeconcludedthatthegreensynthesisofsilvernanoparticlesusingdurianrindisasaneco-friendlyandinexpensivemethodandDRAgNPscouldbeusedinthefieldofwatertreatment,pharmaceuticals,biomedicine,biosensorandnanotechnologyinnearfuture.Keywords:Duriozibethinus;greensynthesis;silver;nanoparticles;antibacterial;cytotoxicity;photocatalytic.


51


INVESTIGATIONONANTIOXIDANTPROPERTYOFMALAYSIANGREENMUSSELS

KrishnamoorthyVenkateskumar1*,LeowYuChuen1,SivayogiVengadan1,SubramaniParasuramanS1,RajuGunasunderi2,SadasivamKathiresanS1,OmarBinSowkatAli2

1.FacultyofPharmacy,AIMSTUniversity,Semeling08100Malaysia

2.SchoolofDistanceEducation,UniversitiSainsMalaysia,PenangMalaysia*E-mail:[email protected]

ABSTRACT

Green-lippedmussel,PernaviridisL.,isnativetotheIndo-PacificregionandisextensivelyculturedinmanyAsiancountriesasasourceofcheapanimalprotein.Thepropertiesofgreenmusselmayvarydueto itsgeographical location,and localmarineenvironment.Reportssuggest thatGreenmussels havebeen reported for their biomonitoring, anti-inflammatory, antiviral activity and itsantioxidantpropertyandtheMalaysianMusselshavenotbeenextensivelystudied.TheobjectiveoftheworkistoassesstheantioxidantpotentialofMalaysiangreenmussels(P.Viridis).Themusselswereextractedbyusingwaterandmethanol as solvents andbyMicrowave -assistedextraction(MAE)andAnimalTissueHomgenization(ATH)methods.TheantioxidantpropertyoftheextractswasassessedbyDPPHscavengingandHydrogenperoxidescavengingactivitymethodsatvariousconcentrations using Ascorbic acid and Butylated hydroxyl toluene (BHT) as standards. Thepercentage yield of extracts was higher in extracts prepared by MAE than ATH method. Thebiochemical analysis of extracts showed the presence of phenolic compounds and polyphenols.Resultssuggestthatthemethanolicextractswerefoundtoexhibithighest%ofscavengingactivitythanaqueousextractsbutlessascomparedtostandardsINDPPHscavengingActivitymethod.Itwas also observed that the scavenging activity was found to increase with an increase inconcentrationsofextracts.TheresultsofhydrogenperoxidescavengingactivitywereexpressedasthepercentageofscavengingofH2O2,IC50valuesandasdose-dependentcurve.Theresultsclearlysuggest that the highest percentage of hydroxyl radical activity was observed for methanolicextractsfollowedbyaqueousandethanolicextracts.Theresultsalsoprovedthattheantioxidantpropertywerealsofoundtobelessincomparisontosimilarstudiesreportedearlierandthismaybeattributedtoitsgeographicallocation,andlocalmarineenvironment.Itcanbeconcludedthatthe methanolic extract of P. Viridis was found to exhibit significant antioxidant property thanaqueousextracts inboth themethodsand thispotential couldbeattributed to thepresenceofpolyphenols in extracts. Further, studies are essential to provide insights into the molecularmechanisminvolvedintheantioxidantpropertyofthetestedextracts.Keywords:GreenMussels,P.Viridis,Antioxidantproperty.


52


SIMULTANEOUSVOLTAMMETRICDETERMINATIONOFCAFFEINEANDPARACETAMOLUSINGCARBONPASTEELECTRODEMODIFIEDWITHCAFFEINEIMPRINTEDPOLY(XYLENOLORANGE)–COMPARATIVEVOLTAMMETRICSTUDIESOFCAFFEINE

RiskiaChandraWidianti*,IndraNoviandri

AnalyticalChemistryResearchGroup,DepartmentofChemistry,InstitutTeknologiBandung,Jl.GaneshaNo.10,Lb.Siliwangi,KotaBandung,JawaBarat40132


ABSTRACT

Caffeine(1,3,7-trimethylxanthine)isacentralnervoussystemstimulantthatcanimprovetheabilityofmentalactivityandmusclework.Thestimulanteffectofcaffeineisusedtoincreasetheactivityofcertaindrugs.Oneofthoseisparacetamol(N-acetyl-p-aminophenol)whichiswidely used as an analgesic. The combination of paracetamol with caffeine is believed toincreasetheanalgesicactivityofparacetamol.Voltammetryisoneofanalyticalmethodsthatcan be used to determine caffeine and paracetamol simultaneously. However, caffeineconcentrationindrugismuchsmallercomparedtothatofparacetamol.Therefore, it isstillnecessary to modify the working electrode to improve its sensitivity for voltametricdetermination of caffeine. Caffeine imprinted Poly (xylenol orange)modified carbon pasteelectrode (CPE-MIP) had been developed to improve caffeine signal for simultaneousdeterminationofcaffeineandparacetamolbydifferentialpulsevoltammetrytechnique(DPV).CPE-MIPwaspreparedbyelectropolymerizationofusingcyclicvoltammetrytechniqueusingcaffeineastemplate.Theprocesswasconductedin0.1MphosphatebuffersolutionofpH7containing1mMxylenolorangeand1mMcaffeine.Optimizationoffactorsaffectingelectrodefabricationhasbeenconducted.Comparativeelectrochemicalstudiesbetweenbare,CPE-NIP,and CPE-MIP for determination caffeine showed that the modification can increase thesensitivityandacceleratetheelectrontransferonelectrodesurface.Keywords:Poly(xylenolorange),caffeine,paracetamol,cyclicvoltammetry,drugCombination


53


DEVELOPMENTANDVALIDATIONOFSIMPLESIMULTANEOUSANALYSISFORAMLODIPINEANDGLIBENCLAMIDEBYNON-DERIVATIZATIONHPLC-FLUORESCENCE

FebrinaAmeliaSaputri1*,AnisahtulAlawiyah1,AyuBrillianyFirsty1,SandraMegantara1,Arif

SatriaWiraKusuma3,TaofikRusdiana4,AliyaNurHasanah1,Mutakin1,IngridSuryantiSurono5,RizkyAbdulah2

1DepartmentofPharmaceuticalAnalysisandMedicinalChemistry,FacultyofPharmacy,UniversitasPadjadjaran,Jl.RayaBandungSumedangKm.21,Jatinangor45363,Indonesia.2DepartmentofPharmacologyandClinicalPharmacy,FacultyofPharmacy,Universitas

Padjadjaran,Jl.RayaBandungSumedangKm.21,Jatinangor45363,Indonesia.3DepartmentofBiologicalPharmacy,FacultyofPharmacy,UniversitasPadjadjaran,Jl.Raya

BandungSumedangKm.21,Jatinangor45363,Indonesia.4DepartmentofPharmaceuticalandFormulationTechnologyFacultyofPharmacy,Universitas

Padjadjaran,Jl.RayaBandungSumedangKm.21,Jatinangor45363,Indonesia.5FoodTechnologyDepartment,FacultyofEngineering,BinaNusantaraUniversity,Jl.K.H.

SyahdanNo.9,Kemanggisan,Palmerah,Jakarta11480,Indonesia.*E-mail:[email protected]

ABSTRACT

Studieshave shown thatabout65%ofdiabeticshavehypertension.Treatment fordiabeticpatientswithhypertension is usually given a combinationof drugs such as amlodipine andglibenclamide.TheaimofthisstudywastodevelopandvalidatesimplesimultaneousmethodforseparationofamlodipineandglibenclamideusingHPLCwithfluorescencedetectorwithoutderivatization.OptimumconditionwasobtainedusinganRP18(125mmx4mm,i.d)andguardcolumnRP18(4mmx4mm,i.d)withmobilephasecompositioncontainingacetonitrileandphosphatebufferpH3.0usinga20:80gradientconditionatflowrate1.0ml/minmeasuredat361nmλexcitationandat442nmλemissionandforamlodipineandat235nmλexcitationand at 354nmλ emission for glibenclamide. The analysis of amlodipine and glibenclamidedemonstratedavalidresultwithr2value0.999,recoverieswere100,036%and98,21%,%RSDwere 0,508% and 6,55%, respectively, detection limit were 0,055 μg/ml and 0,104 μg/ml,quantificationlimitwere0,166μg/mland0,316μg/ml,respectively.Hence,accuratemethodofseparationforamlodipineandglibenclamideusingHPLCwithfluorescencedetectorwithoutderivatizationhasbeenvalidated.Keywords:amlodipine,glibenclamide,simultan,HPLC-fluoresence,nonderivatization


54


FORMULATIONANTI-ALOPECIAOFANGIOPTERISEVECTAEXTRACT

DolihGozali1,AliyaNurHasanah2,ResmiMustarichie2,*

1DepartmentofPharmaceutics,FacultyofPharmacy,UniversitasPadjadjaran,JlRaya

BandungSumedangkm21JatinangorIndonesia453632DepartmentofPharmaceuticalAnalysisandMedicinalChemistry,FacultyofPharmacy,

UniversitasPadjadjaran,JlRayaBandungSumedangkm21JatinangorIndonesia45363*E-mail:[email protected]

ABSTRACT

Herbalandsyntheticcosmeticproductshavebeendevelopedtounravelproblemofhairloss,yetsyntheticsarepotentialtogivesideeffects(e.g.localirritation),whilstherbalproductsaregenerally safer.Angiopterisevecta (Sundanese:Pakumunding )extracthasbeenproved tohaveananti-alopeciaactivity.Thepurposeofthisstudywastoformulatenutraceuticalhairtonic(s)whichwasstable,effectivetowardshairgrowth,andsafe.A.evectawasextractedwithethanol and formulated into varied concentrations i.e. 7.5, 10 and 15% in the hair tonicpreparation. Physical stability test performedwas cycling test, storage in high temperature(40°C±2°C),roomtemperature(25°C±2°C),andlowtemperature(4°C±2°C).Safetytestwasperformedby9volunteers’upperhands.Resultsshowedthehairtonicwasstableinstorage,except in low temperature (4°C ± 2°C). In addition to giving hair growth activity, all of theformulas had greater activity than synthetic drug i.e. minoxidil 2.5%. These hair tonicpreparations were stable, effective, safe and did not irritate the skin. The most optimalformulationwasformulaextractconcentrationof10%.Keywords:Angiopterisevecta,anti-alopecia,formulation,hairtonic,pakismunding


55


SACRANHYDROGELFILMCONTAININGEPIDERMALGROWTHFACTORFORWOUND

HEALINGAPPLICATION

NasrulWathoni1,4,*,TaofikRusdiana1,ArvendaRezkyPratama1,MaikoOkajima3,KeiichiMotoyama4,HidetoshiArima4

1DepartmentofPharmaceutics,FacultyofPharmacy,UniversityPadjadjaran,Sumedang45363,Indonesia

2DepartmentofPharmacologyandClinicalPharmacy,FacultyofPharmacy,UniversityPadjadjaran,Sumedang45363,Indonesia

3JapanAdvancedInstituteofScienceandTechnology(JAIST),1-1Asahidai,Nomi-shi,Ishikawa,923-1292,Japan.

4GraduateSchoolofPharmaceuticalSciences,KumamotoUniversity,Kumamoto8620973,Japan


ABSTRACTRegenerativetherapywithepidermalgrowthfactor(EGF)isapotentialtherapyforrecoveringtheskintissuedamageinchronicwound.EGFacceleratesepidermalregenerationanditiswidelystudiedasawoundhealingagent.However,thespecialcarrierfortopicaladministrationofEGFisurgentlyneededtodeliverEGFonthewoundsiteduetoitseasilydegraded.Inpreviousstudy,sacranhydrogelfilm(Sac-HF)hadanactivityasawoundhealingandagoodabilityasadrugcarrier.Inthisstudy,wefabricatedandcharacterizedphyscochemicalpropertiesofSac-HFcontainingEGF(Sac/EGF-HF)suchasthickness,swellingratio,degradability,tensilestrength,microscopicmorphology,thermalandamorphousstudies.Inaddition,weinvestigatedthecellmigrationandproliferationonSac/EGF-HFinvitro.ScanningElectronMicroscopeconfirmedthatSac/EGF-HFwassuccessfullypreparedbycastingmethodwithhomogeneousfilm.Inaddition,EGFsignificantlyincreasedthethickness,tensilestrengthanddegradabilityofSac/EGF-HFcomparedtoSac-HF.Sac/EGF-HFhadalowerswellingabilitycomparedtoSac-HGF,thisresultwascorroboratethetensilestrengthresult.Interestingly,X-RayDiffractionandDifferentialScanningCalorimetricresultsshowedthatSac/EGF-HFhadanamorphousshapewithhighthermodynamicactivity.TheInVitrostudiesrevealedthatSac/EGF-HFsignificantlyimprovedcellmigrationandproliferationactivitiescomparedtoSac-HF.TheseresultssuggestthatEGFhasthepotentialtopromotethewoundhealingabilityofSac-HGF.Keywords:Sacran,regenerativetherapy,hydrogelfilm,EpidermalGrowthFactor,cellmigration


56


EFFECTOFSOLVENTSANDMACERATIONTIMEONANTIOXIDANTACTIVITYOFMANGOSTEENPEEL(GarciniaMangostanaL.)EXTRACT

AndriKusmayadi1,2*,LovitaAdriani1,Abun1,Muchtaridi3,UjangHidayatTanuwiria1

1DepartmentofAnimalScience,UniversitasPadjadjaran,Bandung–SumedangStreetKM.21,Jatinangor-45363,Sumedang

2DepartmentofAnimalScience,UniversitasPerjuangan,PembelaTanahAirStreetNo.177,Tawang-46115,Tasikmalaya

3DepartmentofPharmacy,UniversitasPadjadjaran,Bandung–SumedangStreetKM.21,Jatinangor-45363,Sumedang


ABSTRACTExtractionisconductedtoextracttheimportantcompoundsinmangosteenpeelusingsolventsatthedifferenttime.Extractiontimeneedstobecalculatedsothatthebioactivecompoundcanbeextractedoptimallyusingthemostefficientsolvent.Theextractionofmangosteenpeelwasconductedbysoakinginsolventatparticulartime(1-2dayingeneral)withoutheating.Thisstudyaimstoevaluatetheeffectofdifferentsolventsandmacerationtimeonantioxidantactivity of mangosteen peel extract. The present research was conducted by extractingmangosteen peel using ethanol, acetone, ethyl acetate,methanol, hexane, acetic acid andaquadest at 24, 36 and 48h. Antioxidant activity was examined using visible UVspectrophotometerattheparticularwavelength.Theresultshowedthatthetypeofsolventandextractiontime(P<0.01)affectedantioxidantactivity.Acetoneextractofmangosteenpeelisthemostoptimalsolventforantioxidantactivityinextractiontimefor24hours(IC50=9,468+0,324ppm).AfurthertestwasrequiredtoobtainabetterresultusingHPLCmethod.Keywords:antioxidantactivity,macerationtime.mangosteenpeelextract,solvents


57


FORMULATIONOFPALMSHELLACTIVATEDCHARCOAL(ElaeisguineensisJacg)TOOTHPASTEAS

ATEETHWHITENERINNICOTINEADDICTSRATHERTHANSMOKERS

Syamsurizal1*,UceLestari2,Nurhasanah3

1*ChemicalEducationStudyProgram,FacultyofTeacherTrainingandEducation,UniversityofJambi

2,3PharmaceuticalStudyProgram,FacultyofScienceandTechnology,UniversityofJambiKampusPinangMasak,JalanrayaJambi-MaBulianKm15MendaloDarat,Jambi,kodepos36361


ABSTRACTActivated charcoal has the ability to absorb dirt or toxins. The pores formed on this activatedcharcoalwillbindthedustonthetoothsurface.Theeffectivenessofactiveoilabsorptionofpalmshell(ElaeisguineensisJacg)isbetterthantheactivatedcoconutshellcharcoalandwood,becausehighoxygencontent (1).Activeoilabsorptionability, theactivecharcoal isusedasa toothpasteproducttokeepgumandmouthhealthyfornicotineaddicts(smokers)wherenicotinecandamagetheliningofthebonesandtissuesintheteethcausingtheteethtoturnyellow(2).Thisstudyaimstodeterminetheeffectivenessofpalmshellactivatedcharcoal(ElaeisguineensisJacg)toothpasteasateethwhitenerinnicotineaddictsratherthansmokers,andtodetermineformulationhasthebestphysicalpropertiesandstableduringstorage.Activatedcharcoalthatusedinformulationswitha concentration of 12.5% and different concentrations of carbomer 934 and tween 80. Theevaluationofphysicalpropertiesoftoothpasteinclude:organoleptictest(black,mint,ratherthick,half solid), homogeneity ( homogeneous), pH measurement (7,28), viscosity (49.142 cps), flowproperties(plastis),dispersion(3,41cm),foamheight(2,83cm),cyclingtest(thereisnotseparation)andtheeffectivenessofpalmshellactivatedcharcoal(ElaeisguineensisJacg)toothpasteasateethwhitenerinnicotineaddictsratherthansmokers.Adescriptivestudyproduceddatasuggestthattoothpastesthathavebetterphysicalpropertiesandarestableduringstorageareformula2withacarbomer934baseof1%andtween80of2%.Alltoothpasteformulashavethetheeffectivenessofpalmshellactivatedcharcoal(ElaeisguineensisJacg)asateethwhitenerinnicotineaddictsratherthansmokerswithawhitishlevelof8(grayishwhite)andastatisticallysignificantfindingrequiresaP-valueof0.05orlesswhenthepowerisatleast95%.Keywords:toothpaste,activatedcharcoal,nicotineaddicts


58


THEEFFECTIVENESSOFLOTIONPREPARATIONSASEMOLLIENTSFROMPUREPALMOILAND

CRUDEPALMOIL

UceLestari1*,Syamsurizal2,FadhilahYahya3

,1,3PharmaceuticalStudyProgram,FacultyofScienceandTechnologyUniversityofJambi2ChemicalEducationStudyProgram,FacultyofTeacherTrainingandEducation,Universityof

JambiKampusPinangMasak,JalanrayaJambi-MaBulianKm15MendaloDarat,Jambi,kodepos36361


ABSTRACTPalmoilisnaturallyredduetothehighcontentofbetacarotene.Mostofthefattyacidscontainedincrudepalmoilaresaturatedfattyacidsnamelypalmiticacid32-59%whilepurepalmoilislinoleicacid5-11%whichfunctionsasamoisturizerforheskin.Basedonthepotentialofpalmoil,cosmeticpreparationsaremadeintheformoflotionsasemollientsontheskinwithaconcentrationof5and15%usingoilinwaterbase.Thisstudyaimwastocomparedtheeffectivenessoflotionpreparationsasemollientsfrompurepalmoilandcrudepalmoil.Descriptivedatastatedthatofthe10panelistswhotestedtheirskinmoistureusingskinanalyzer,itwasproducedthat15%crudeoillotionand15% pure palm oil lotion had amoisture content exceeding 50%withmoist skin category. Thisdifferencecomesfromthecontentofsaturatedfattyacidsthatpalmoilhas.Keywords:PalmOil,Lotion,Emollients


59


VARIATIONCONCENTRATIONOFCITRICACIDASACIDSOURCESONTHEPHYSICAlPROPERTIESOFTHEPERICARPMANGOSTEEN(GarciniamangostanaL)EXTRACTS

EFFERVESCENTTABLET

IndingGusmayadi*,PramulaniMulyaLestari

FacultyofPharmacyandSciences,UniversitasMuhammadiyahProf.DR.HAMKAJl.DelimaII/IVPerumnasKlender,DurenSawit,JakartaTimur,14360


ABSTRACT

Citric acid is an acid source in effervescent tabletwhich reacts rapidlywith solvent. It canincrease dissolving time. In this research, extract was made into effervescent tablet. Theresearchaimedatknowingincreasedconcentrationofcitricacidasacidsourcecanimprovethephysicalcharacteristicofeffervescenttablet.Pericarpmangosteenmaceratedbywaterassolvent,andtheextractwasmadeintopowderbyspraydryermethod.Dryextractwasmadeinto5formulaseffervescenttabletwiththedifferentconcentrationsofcitricacid,15%,20%,25%, 30%and35%as acid sources. The evaluationof tablets quality includedorganolepticevaluation,weightuniformity, sizeuniformity,hardness, friability,anddissolving times.Theresult of dissolving time was subquently 10.50±0.57, 4.52±1.04, 3.32±0.13, 3.13±0.14, and3.42±0.14minutes.Thevalueofdissolvingtimebetweentheconcentrationofcitricacidweresignificanlydifferent(ANOVA,p<0,05).Itcanbeconcludedthatthelevelofconcentrationofcitricacidaffectedondissolvingtimeofthepericarpmangosteen(GarciniamangostanaL.)dryextracteffervescenttablets.Keywords:Mangosteen,Effervescenttablet,Citricacid


60


INVITROANTIOXIDANTACTIVITYOFGARDENCROTON(CODIAEUMVARIEGATUM(L.)RUMPH.EXA.JUSS.)USINGDPPH(2,2-DIPHENYL-1-PICRYLHYDRAZYL)METHODANDPHYTOCHEMICAL

ANALYSISUSINGGC-MS

Nurwanti*,SitiWanda,Aritonang,Warnetty,Hefni,Puspayani

AkademiFarmasiHangTuahJakartaJl.Farmasino.1BendunganHilir,JakartaPusat10210


ABSTRACTGardenCroton(Codiaeumvariegatum(L.)Rumph.exA.Juss.)isaneasilygrownplant,hasavariousshapeandcolorfulleaveswhichusuallyusedasanornamentalplant.Theaimsoftheresearchwereto determine antioxidant activity of Croton and its phytochemical content. The crude drugwasextractedusing96%ethanol.AntioxidantactivitywasperformedusingDPPHfreeradicalscavengingmethodandqualitativeanalysiswasdeterminedusingGC-MS.Phytochemical screening showedthatextractcontainsflavonoids,tannins,andsaponins.BasedontheinvitroantioxidanttestusingDPPH,IC50ofcrotonextractwas797.61ppmwhiletheIC50ofascorbicacidwas8.97ppm.BasedonanalysisofthechemicalcompoundofcrotonusingGC-MS,itobtained10compoundswithpercentofquality(degreeofsimilarity)averagemorethan90%.Themaincompoundswerepentadecadien-1-ol 34.15% and hexadecanoic acid 21.14%. Croton leaves extract was categorized as lowantioxidantactivityandcontained(6Z,9Z)pentadecadien-1-olandhexadecanoicacidastheprimarychemicalcontent.Keywords:GardenCroton,antioxidant,GC-MS,CodiaeumVariegatum


61


CYTOTOXICACTIVITYOFVoacangafoetida(Bl.)K.SchumLEAVESONT47DBREASTCANCERCELLLINE

AdrianiSusanty1,3,*,Dachriyanus2,Yanwirasti3,FatmaSriWahyuni2,SholehaUlfaRahim1

1SchoolofPharmacy,SekolahTinggiIlmuFarmasiRiau,Pekanbaru,28293,Riau,Indonesia,

282932FacultyofPharmacy,AndalasUniversity,LimauManisStreet,Padang,West

Sumatra,Indonesia.3FacultyofMedicine,AndalasUniversity,LimauManisStreet,Padang,West

Sumatra,Indonesia.*E-mail:[email protected]

ABSTRACT

Cytotoxicactivityisassociatedwithitspotentialasananticancer.Primarymetabolite(palmiticacid)andsecondarymetabolites(indolealkaloid,steroidandterpenoid)havebeenknowntohave cytotoxicactivity. These compoundsare contained inVoacanga foetida (Bl.)K.Schumleaves extract. However, there is very lack information of cytotoxic study from V. foetidaleaves.TheaimofthisresearchwastodeterminethecytotoxicactivityofV.foetidaleaves.Inorder to achieve these objectives, the study have been done through a series of stepspreparation included extraction and determination of cytotoxic activity with MTT AssayMethod on T47D breast cancer cell line. Extractionwas performed byMacerationMethodbasedonthepolarityofthesolventbyusingnon-polar(n-hexane),semipolar(ethylacetate)andpolar (ethanol) solvents. From this study, the IC50valueofhexane, ethyl acetateandethanolextractare3.15;3.30and16.02µ/mLrespectively.ItcanbeconcludedthatV.foetidaleavesispotentialtobedevelopedasanewalternativeanticancer.Keywords:Voacangafoetida(Bl.)K.Schum,cytotoxic,T47Dcellline


62


COMPARISONOFCARBOPOL934AND941ASTHICKENERONDIFFUSIONRATEOFKETOCONAZOLEMICROEMULSION

RahmahElfiyani*,YudiSrifiana,NauvalAudiaAndamdewi

FacultyofPharmacyandScience,UniversityofMuhammadiyahProf.DR.Hamka,Jakarta,

Indonesia*E-mail:[email protected]

ABSTRACT

The use of thickener can increase the viscosity of themicroemulsion because it can affectphysicalstabilityofthesystemandthediffusionrateofactivesubstance.Thisstudyaimedtocompare the diffusion rate of ketoconazole in microemulsion containing carbopol 934 orcarbopol 941 as a thickener. Ketoconazole microemulsion was made into 7 formulas withvariousconcentrationsineachtypeofthickener,i.e0.1%,0.2%,0.3%,andoneformulawithoutthickener. Each formula was evaluated includes organoleptic, pH measurement, viscosity,particle size, potential zeta, surface tensionmeasurement, and diffusion test. The physicalpropertiesofketoconazolemicroemulsionshowedliquidtogelform,yellowincolor,specificodor,pHwasintherangeof7.00-7.65,viscosityrangedfrom2196-26037cps,particlesizewas13.6-42.2nm,zetapotentialwas-9.12--18.44mV,andsurfacetension30.7-64dyne/cm.Thediffusionkineticsobtainedfollowedbythehiguchiequationfortheuseofthetwothickener,whiletheketoconazolemicroemulsionwithoutthickener(F1)followedtheKorsmeyer-peppasequationwithadiffusionrateof9.444.Thediffusionrateofketoconazolemicroemulsionwithcarbopol934(F2,F3,F4)were4.354,4.638,and4.847,respectively.Meanwhile,thediffusionrateusingcarbopol941(F5,F6,F7)were4.695,4.742,4.751,respectively.Itcanbeconcludedthatthediffusionrate(sig˂0,05,)ofketoconazoleinmicroemulsioncontainingcarbopol941was faster than diffusion rate of microemulsion containing carbopol 934 at the sameconcentrationinthemicroemulsionsystem.Keywords:microemulsion,thickener,carbopol934,carbopol941,diffusionrate


63


PROTEASEACTIVITYANDCHARACTERIZATIONOFBROMELAINEXTRACTOFPINEAPPLE(Ananascomusus(L.)Merr)CROWN

NyiMekarSaptarini1*,DriyantiRahayu1,SriAgungFitriKusuma2

1DepartmentofPharmaceuticalAnalysisandMedicinalChemistry,FacultyofPharmacy,UniversitasPadjadjaran,JlRayaBandungSumedangkm21JatinangorIndonesia45363

2DepartmentofPharmaceuticalBiology,FacultyofPharmacy,UniversitasPadjadjaran,JlRayaBandungSumedangkm21JatinangorIndonesia45363


ABSTRACT

Pineapple (Ananas comusus (L.)Merr) has a high bromelain contentwith protease activitywhich hydrolyses proteins. Bromelain is distributed to all parts of pineapple, including thepineapplecrown,whichisagriculturalwastethathasnotbeenutilizedproperly.Thepurposeof this studywas todetermine theproteaseactivityand thecharacterofbromelainextractfrom the pineapple crown. The bromelain extract was obtained through the precipitationprocess with ethanol. UV spectrophotometer was used to determine protease activity bymeasuringthetyrosineconcentrationwhichproducedfromhydrolysisofcaseinasasubstrate.ThebromelaincharacterwasdeterminedbyproteaseactivityinvariationsinpH,temperature,andsubstrateconcentration.Theresultsshowedthatproteaseactivityof7.72±0.45IU/mL.OptimalactivitywasreachedatpH5and55°C,KMas1.00μg/mL,Vmaxas8.11IU/mL,andKcatas0.03/sec for caseinas substrate.Thebromelainextractofpineapplecrownhasproteaseactivitywiththespecificcharacter.

Keywords:precipitation,casein,UVspectrophotometer,specificcharacter


64


DESIGNOFINDICATORSTRIPSWITHCELLULOSEBASEFORDETECTIONOFPARACETAMOLINHERBALMEDICINE

DriyantiRahayu*,RimadaniPratiwi,TriNenciSulistyoPuri,AliyaNurHasanahPharmaceuticalAnalysisandMedicinalChemistryDepartment,FacultyofPharmacy

UniversitasPadjadjaran.Jl.RayaBandungSumedangKm.21Jatinangor45363,Indonesia*E-mail:[email protected]

ABSTRACT

Theadditionofchemicalsintoherbalmedicineisforbidden,howeverherbalmedicineproductscontainingchemicalswerestillfoundonthemarket.Oneofthosechemicalsthatwasfoundinherbalmedicineisparacetamol,whichwasaddedtoenhancethepainreliefeffectofherbalmedicine. Indicator strip is one of the simplest methods for detecting chemicals in herbalmedicineproduct.Indicatorstripsaregenerallymadewithaplasticbasethatisconsideredlessenvironmentally friendly. This study aims to develop a paracetamol indicator strip withenvironmentallyfriendlypolymer-based.Inthisstudy,theindicatorbasewasproducedfromcelluloseofbananapseudostem.Theindicatorwasthendevelopedwithsolventimpregnationmethod by using ferric chloride, Folin-Ciocalteu, and quinhydrone as paracetamol specificreagents. The result of indicator strip testing gave minimum detection limit (LOD) ofparacetamolinherbalmedicineat5,000ppm.Moreover,biodegradabilitystudyshowedthatthestripswereeasilydegradedinsoilmedia,withbiodegradabilitypercentagereach35.801%in14days.Inconclusion,thebananapseudostemcellulose-basedindicatorstripscanbeusedas an alternative of environmentally friendly detection method of paracetamol in herbalmedicine.Keywords:indicatorstrips,paracetamol,cellulose,herbalmedicine


65


REDGINGER(ZingiberofficinaleRoscoevar.SuntiVal)EXTRACTSINHIBITSIĸBαPHOSPHORYLATIONINTHENFĸBINFLAMMATORYPATHWAYOFNEONATALRAT

CARDIOMYOCYTES

SitiWakhidatunSuciyati1,,NengFisheriKurniati1,Sukrasno1,YasushiFujio2,IKetut

Adnyana1*

1DepartmentofPharmacologyandClinicalPharmacy,SchoolofPharmacy,InstitutTeknologi

Bandung,JalanGaneca10,Bandung,JawaBarat40134Indonesia2LaboratoryofClinicalScienceandBiomedicine,GraduateSchoolofPharmaceuticalSciences,

OsakaUniversity,1-1Yamadaoka,Suita,Osaka565-0871Japan*E-mail:[email protected]

ABSTRACT

Theroleof inflammation incardiovasculardisease isundeniablydefinite.NFκB,a familyoftranscriptionfactorshasamajorroleininflammationoftheheartandbloodvessels.ActivationoftheNFκBinflammatorysignalingpathwaycausedthereleaseofproinflammatorycytokinessuchasTumorNecrosisFactorα(TNFα), Interleukin1β(IL-1β),andInterleukin6(IL-6).Redginger commonly used as spice in Indonesia is frequently used as a traditional medicine.Previousstudiesshowedredgingerexhibitantioxidantandanti-inflammatoryactivityinsomeinvitromodels.Thehighcontentofgingerolandshogaolinthisrhizomeisthoughttobethecause of the anti-inflammatory effect. The molecular anti-inflammatory mechanism of redgingerhasnotbeendeterminedespeciallyinthecardiovascularsystem.Theaimofthisstudyistodeterminetheanti-inflammatoryactivityofredgingerrhizomeextractoncardiomyocytecells using IĸB α phosphorylation as an activation parameter of the NF ĸB inflammatorypathway. Red ginger rhizome extracts were obtained using gradual soxhletation method.Cardiomyocyteswereisolatedfromneonatalratsage1-3days.Starvedcardiomyocyteswereculturedwithextractsobtainedfromredgingerrhizomefor4hourstheninducedbyTNFα10ng/ml for 5 minutes. Harvested cardiomyocytes then were used in Western blotting test.ResultsshowedhexaneextractcollectedfromredgingerrhizomesgavethehighestinhibitionofIĸBαphosphorylationfollowedbyethylacetateextractandethanolicextractinthesameconcentrations albeit inferior compared to the comparator drug (BAY 11-7082). Red gingerrhizomeshexaneextracts inhibitedIĸBαphosphorylationinadosedependentmanner.Theresultsof thisstudyservedasstartingpointofprobableprotectiveroleofredginger inthecardiacandholdpotentialstobedevelopedastherapeuticsagent.Keywords:cardiomyocyte,NFĸBpathway,redginger.


66


ANTIMICROBIALACTIVITYANDPHYTOCHEMICALPROPERTIESOFACTINODAPHNEGLOMERATALEAVESEXTRACT

HKuspradini*,I.Suharyani*,JManalu

FacultyofForestry,MulawarmanUniversity,JlKiHajarDewantaraKampusGunungKelua,

Samarinda,EastKalimantan,75116,Indonesia*E-mail:[email protected]

ABSTRACT

AntimicrobialactivityofActinodaphneglomerataleavesoilwereestablishedtoinvestigateitspotential uses. This study aimed to determine the antimicrobial activity and chemicalcompoundsbyphytochemicalanalysisofActinodaphneglomerataleaves.Theextractionwasperformedbymacerationmethodusingn-hexanesolvent.Theantimicrobialactivitywastestedby agar diffusion method against Candida albicans, Staphylococcus aureus, StreptococcusmutansandStreptococcussobrinus.Then-hexanecrudeextractswastestedforitsantimicrobialactivityusing125,250and500μg/wellofconcentrations.Basedonphytochemicalanalysis,itshowed thatn-hexane extract ofA. glomerata leaves contained alkaloids, triterpenoid andcarbohydrates.TheextractinhibitedalltestedmicroorganismsandthebestinhibitionzonewasshownagainstS.sobrinus(19.56mm)atconcentration500μg/well.Keywords:Actinodaphneglomerata,n-hexaneextract,Phytochemical,Antimicrobial.


67


PRELIMINARYTESTOFUTILIZATIONOFAspergillusnigerINTHEPROCESSOF

BIOTRANSFORMATIONOFGERANIOLANDITSIDENTIFICATIONWITHGASCHROMATOGRAPHY-MASSSPECTROMETRI(GC-MS)

YelfiAnwar1,*,ElvinaDhiaulIftitah2,PartomuanSimanjuntak3,ShirlyKumala4

1. ProgramDoktoral,FakultasFarmasi,UniversitasPancasilaJakarta2. FakultasKimia,UniversitasBrawijayaMalang

3. PusatPenelitianBioteknologi,LIPI–Cibinong,Bogor4. FakultasFarmasi,UniversitasPancasilaJakarta


ABSTRACT

EssentialOils(EOs)havegainednewinterestinseveralaspects.Asanaturalproduct,essentialoilshave attractivephysicochemical characteristicswithhigh added values that are environmentallyfriendly.EOalsohasdiverseandrelevantbiologicalactivities.AnumberofstudieshavehighlightedtheantimicrobialeffectsofEOevenonmulti-resistantbacteria.BecauseofthecomplexchemicalcompositionofEO,EOhasabroadspectrumofbiologicalandantimicrobialactivity(antibacterial,antifungal,antiviral,pestcontrol,insectrepellent).Inthepharmaceuticalfield,EOisincludedinthecompositionofmanydosageforms(capsules,ointments,creams,syrups,suppositories,aerosols,gelsandsprays).Cymbopogonnardus(L.)Rendleisonetypeofplantthatcontainsessentialoils.Usuallyknownassitronella.SitronellaorCymbopogonnardus(L.)RendleisoneoftheCymbopogonspecies with quintessence oil which is widely used in the production of citronella, geraniol,citronelol, food,beverages, fragrances, soaps,body careproducts andpharmaceuticalproducts.Many studies have reported C. nardus had antifungal and antimicrobial properties.Biotransformationstudyofgeraniol,nerolandcitralbyAspergillusniger,ithasbeenreportedthatthemainbioconversionproductsobtainedfromgeraniolandnerolbyliquidcultureofA.nigerarelinaloolandα-terpineol.ThisstudyaimstoutilizeAspergilusnigerforthebiotransformationprocessofgeranioltoobtainothercompounds.TheresultsofidentificationwithGC-MSshowedthatthebiotransformationofgeraniolwaslinaloolandalpha-terpineol,whichelutedandwithpeakareasrespectively;12.657and145.009.110;16.340and4.567.385(day-1);12.820and409.287.950;16.422and35.681.840(day-7)Keywords:Aspergillusniger,Biotransformation,Geraniol,GC-MS.


68


APPLICATIONOFCOMMONGREENBOTTLEFLY(Luciliasericata,Meigen1826)LARVAEEXTRACTFORINCISIONWOUNDTREATMENTSINRATS

MadihahMadihah*,LisdaMawarniSihotang,DesakMadeMalini,

WawanHermawan

DepartmentofBiology,FacultyofMathematicsandNaturalSciences,UniversitasPadjadjaran,Jl.RayaBandung-SumedangKm.21Jatinangor45363

WestJava,Indonesia.*E-mail:[email protected]

ABSTRACT

Luciliasericatalarvaehasbeenexploitedinmaggotdebridementtherapytopromotewoundhealing since the 19th century following the emergence of an antibiotic-resistant strain ofbacteria. Whole body extracts and hemolymph of L. sericata larvae shown antibacterialproperties.ThisresearchaimstoexaminetheethanolextractfromwholebodyofL.sericatalarvaetoacceleratethewoundhealingontheskinofmaleWistarrats(Rattusnorvegicus).Themethodwasacompletelyrandomizeddesignwithsixtreatmentsandfourreplicationseach.The incisionwoundcreatedatthedorsolateral regionofshavenskinat±1cm2usingsterilescissors. The extract at concentration 5, 10 and 15% in olive oil were applied topically towoundedrats,aswellasBetadine®forthereferencegroup.Forpositivecontrolonly,oliveoilappliedtowoundedrats,asfornegativecontrolwasnon-treatedwoundedrats.Thetreatmentwasdonetwiceadayfor14days.Atday15th,thewoundedsiteharvested,fixedin10%NBF,embedded in paraffin, and sectioned at 5-7 µm, then stained with hematoxylin-eosin ortrichrome Heidenhain’s Azan for histological examination. The results showed that topicalapplicationoftheL.sericatalarvaeextractatconcentration10%wassignificantlyrecoverthewounded skin by enhanced re-epithelization and granulation tissue, as well as increasedcapillary number and collagen density than other treatments (p<0.05). Overall, our datasupporttheL.sericata larvaextractasanagenttoacceleratethewoundhealingprocessonratsskin.Keywords:Luciliasericata,rats,skinwounds,topicalapplication.


69


FORMULATIONANDEVALUATIONOFCAFFEINEANTI-CELLULITEGELWITHADDITIONOFGLYCOLICACIDASENHANCER

YolaDesneraPutri*,NovitariaBrSembiring,SohadiWarya

DepartmentofTechnologyPharmacy,FacultyofPharmacy

SekolahTinggiFarmasiIndonesia(STFI),Jl.Soekarno-HattaNo.354,Bandung40266*E-mail:[email protected]

ABSTRACT

Celluliteoccursdue tochanges inadipose tissue thatcause fibrosklerosis, so skin looks likeorangepeel.Caffeineisasafeandeffectivesubstanceforthetreatmentofcellulite,butitisdifficult topenetrate through thestratumcorneum.Glycolicacidhas theability to increasepercutaneouspenetrationbyreducingcellularcohesionbetweenkeratinocytes.Theaimofthisresearch was to observe the effects of glycolic acid on caffeine penetration in gel. Gelformulationcontainedcaffeine1.5%,triethanolamine0.5%,carbopol2%,metilparaben0.02%,propilparaben0.01%,propilenglikol15%andwater.Formula1doesnotcontainglycolicacid,formula2contains2,5%glycolicacidandformula3contains5%glycolicacid.Evaluationwereconducted for 28 days, including: organoleptic observation, homogenity, pH, viscosity andpenetrationpropertieswhichwereanalyzedbyFranzdiffusioncellfor6hours.Thestudywascompared with anticellulite gel in the market. Caffeine gel were stable at various storagetemperatures(4,28and40oC)andcyclingtest.Caffeinecontentfrompenetrationstudyofgelinthemarket,formula1,2,and3were105.302,173.241,and346.577µg/cm2respectively.The results showed that glycolic acid enhanced the caffeine percutaneous penetrationpropertiesingel.Keywords:cellulite,caffeine,glycolicacid,penetration,enhancer


70


EFFECTIVITYTESTOFETHANOLANDWATEREXTRACTSANDDROPSPREPARATIONSOFREDDRAGONFRUITPEELASBORAXDETECTOR

LilisAdeliah*,MeitasyaAsriUtami,ImamDwiyanMulyana,Ririn

FacultyofPharmacy,UniversitasMuslimIndonesia

Jln.UripSumoharjoKM.5,Makassar,SouthSulawesi,90231*E-mail:[email protected]

ABSTRACT

BoraxisachemicalinwhichtheusehasbeenbannedbythegovernmentofTheRepublicofIndonesiaasadditivesinfoodandhasbeenregulatedinHealthMinister’sRegulationNo.033of2012.Buttheuseofboraxasapreservativeisstilloftendonebysomeirresponsibletraders.TheaimofthisstudywastoStudytheeffectivenessofwaterandethanolextractsanddropspreparationsofreddragonfruitpeelextract(Hylocereuscostaricencis)whichabletodetectboraxboth in the formofpowderor in food. Initial stepof the researchwasextractionbymacerationethanol96%:HCl(9:1)andwater:citricacid(9:1)thenevaluatetheeffectivenessofbothextractsusingboraxintheformofpowderanddropspreparation.Furtherstepwastoformulatebothextractsintodropspreparation.TheEffectivenessofthedropsthenevaluatequalitatively. The indication of detection of borax by observing the change of color.Concentrationofboraxstartedfrom1,0mgto100,0mg.Researchshowedthatbothextractareabletodetectboraxintheformofpowderstartedfrom2,0mgconcentrationofboraxandtheintensityofcolorchangeincreasebytheconcentrationofborax.Whileintheformofdropspreparation both extracts are able to detect borax in the samples started from 20.0 mgconcentrationofboraxandthecolorchangeareintensbyconcentrationofborax.Fromtheresults itcanbeconcludedthat in the formofextractsanddropspreparationsethanolandwaterextractsofreddragonpeelareabletodetectthepresenceofboraxwhetherintheformofpowderorinsamples.Keywords:Borax,DragonFruitPeel,Drops


71


ANTIOXIDANTACTIVITYOFα–AMYRINANDβ-SITOSTEROLFROMBARKHEXANEEXTRACTSOFRHIZOPHORAMUCRONATALAMK.

Nohong1,*,NunukHarianiSoekamto2*,AhyarAhmad2,Sahidin3

1DepartmentofChemistry,FacultyofMathematicsandNaturalSciences,HaluOleoUniversityKendari,Indonesia,93231

2DepartmentofChemistry,FacultyofMathematicsandNaturalSciences,HasanuddinUniversityMakassar,Indonesia,90245

3DepartmentofPharmacy,FacultyofPharmacy,HaluOleoUniversityKendari,Indonesia,93231


ABSTRACT

Isolationand identificationofchemicalcompounds fromann-hexaneextractof thebarkofmangroveplantsRhizophoramucronataLamk.obtainedfromthemangroveforestofKendariBay,SoutheastSulawesiProvinceanditspotentialasananti-oxidanthavebeendone.Isolationandpurificationwereconductbyusingvacuumcolumnchromatography,andelectronicradialchromatography. Molecular structure determination of isolate compounds is based onspectroscopicdata,IR,1H-NMR,13C-NMR(1Dand2D)andbycomparingsimilardatafromtheliterature. Antioxidant activity test was carried out by using DPPH (1,1-diphenyl 2-picryl-hydrazyl)freeradical.Fromthisresearchwasobtainedtwopurecompoundsthatareα-amyrinandβ-sitosterol.Theα-amyrincompoundwasfirstreportedfromthebarkofthisplant.Anti-oxidantactivitytestresultsshowedthatα-amyrinandβ-sitosterolcompoundshadIC50valueare105.69±0.56μMand112.81±0.59μMKeywords:Rhizophoramucronata,isolation,α-amyrin,β-sitosterol,anti-oxidant


72


CAFFEINEVOLTAMMETRICSENSORBASEDONELECTROPOLYMERIZEDMOLECULARLYIMPRINTEDPOLY(GLUTAMICACID)ONCARBONPASTEELECTRODE

NurAyuFitriani*,IndraNoviandri

DepartmentofChemistry,InstitutTeknologiBandung,Bandung40132,Indonesia


ABSTRACT

Caffeine isanelectroactive compound that canbedeterminedbyelectrochemicalmethodssuchasvoltammetry.Oxidationofcaffeineatsurfacebareelectrodegivesrelativelylowcurrentresponse.Inaddition,oxidationofcaffeineusingconventionalbareelectrodeoccursatmorepositivepotential.Inthisresearch,carbonpasteelectrode(CPE)wasmodifiedwithmolecularlyimprintedpolymers(MIP)toimprovecaffeineresponse.ThemodificationCPEsurfacewithMIPwasconductedbyelectropolymerizationprocessusingcyclicvoltammetry (CV) technique inphosphatebuffersolutionofpH7solutioncontainingglutamicacidasfunctionalmonomerandcaffeine as template. Themeasurement of caffeinewas performed using differential pulsevoltammetry(DPV)techniqueinperchloricacidsolutionassupportingelectrolyte.Theeffectsofmonomerandtemplateconcentrations,numberofelectropolymerizationcycles,removingoftemplatecyclesandscanratewasoptimized.Measurementofcaffeineusingthemodifiedcarbon paste electrode (MIP-CPE) showed that modification could increase the currentresponseandshiftpotentialoxidationofcaffeinetomorenegativepotential.Keywords:carbonpasteelectrode,molecularlyimprintedpolymers,glutamicacid,caffeine,voltammetry


73


ANTIOXIDANTPROPERTYOFLIMEPEEL(Citrusaurantifolia(Christm)Swingle)INEMULGEL

Rahmadevi*,LiliAndriani,LidyaEkaPermataSari,SerliMarselina

StikesHarapanIbuJambi*E-mail:[email protected]

ABSTRACT

Limehasbeenafruitwhichisrichinflavonoidsasapolyphenoliccompounds,soitcanbeusedasanantioxidant.Everypartofthelimefruitwasfoundinflavonoids,includinglimepeel.Antioxidantsworksagainstfreeradicals,especiallyonthefaceskincausingblackishcolorwhichisbelievedcanbereducedbyflavonoidscounteract.1Thisstudywasconductedtouseflavonoidsinlimefruitpeelasantioxidantpreparations.Theemulgelhadadvantagesasitisstainless,containsemolient,solubleinwater,goodappearance,easytobespread,easytowash,andtransparent.Extractionmethodhas been used in this researchwasmacerationwith 96% ethanol. Emulgelwere preparedwithvariousconcentrationsoflimepeelethanolextractnamelyF1,F2,F3andF4withconcentrationof0.1%, 0.2%, 0.3% and 0.4% respectively. The emulgel preparations were evaluated includingorganoleptic, IC50 values, particle size, pH, clarity, viscosity, stability and the irritation test.2 Theresults showed that all preparations remained stable at room temperature and still meets therequirements and did not irritate the skin. Antioxidant activity were in the range of strong tomoderateshownbyitsIC50values.Thehighertheconcentrationofextractsinthepreparation,thelowertheIC50value.Keywords:emulgel,Citrusaurantifolia,Antioxidantactivities


74


EXPLORATIONOFPOTENTIALBIOACTIVECOMPOUNDSOFENDOPHYTICMICROBIALCULTUREISOLATEDFROMGALLRUSTSENGON(FALCATARIAMOLUCCANA)Barneby&

J.WGrimes

SopandiSunarya*,NoorRahmawati,AlfiRumidatul

SITHITBJlGaneshaNo10Bandung*E-mail:[email protected]

ABSTRACT

Background: Natural product innovation for antibiotics and antioxidant was very urgent,because of the emergence of antibiotic resistant microorganisms that required inventiveresearchanddevelopmentstrategies.EndophyticfungiisolatedfromgallrustSengonhastheabilitytoproduceantimicrobialandantioxidantcompounds.Theobjectiveofthisresearchwasto investigate the endophytic fungi isolated from gall rust Sengon that produced someantimicrobialandantioxidantcompounds.Methods:Thirty-fourendophyticfungiwereisolatedfrom gall rust Sengon, screened and evaluated their ability to produce antimicrobial andantioxidantcompounds.Results:Antimicrobialactivitieswere found inat leastoneormorepathogenicmicrobial(bacteriaandfungi)tested.InhibitionofBacillussubtilisweredetectedat5 - 46mmand24–82mm formethanol andethyl acetateextract subsequently.WhileinhibitionofPseudomonasaeruginosawas10-63mmformethanolextract;39–103mmethylacetateextractand10–65mmfornhexaneextract.AntifungalactivitytowardsFusariumspshowedinhibitiongrowthwere14%,60%and4.5%formethanol,ethylacetateandnhexaneextractandSchlerotiumspgrowthinhibitionwere18,6%,8,9%and20,3%formethanol,ethylacetateandnhexaneextract.AntioxidantactivityofmethanolandethylacetateextractfungalcultureshowedDPPHradicalinhibitionactivityas44,92%formethanoland71.48%.forethylacetateextract.Meanwhile,nhexaneextractshowedonly28.69%inhibitionforDPPHradicals.Conclusion: endophytic fungi isolated from gall rust sengon were potential for producingantimicrobialandantioxidantcompoundsfromitsculture.Keywords:gallrust,endophyte,fungi,Falcataria,antimicrobial,antioxidant


75


PHARMACOPHOREMODELINGANDMOLECULARDOCKINGBASEDVIRTUALSCREENINGOFB-CELLLYMPOMA2(BCL2)INHIBITORFROMZINCNATURALPRODUCTDATABASE

FauzanZeinMuttaqin*,DinaKharisma,FransiskaKurniawan

SekolahTinggiFarmasiBandung(BandungSchoolofPharmacy)Jl.SoekarnoHattaNo.754

Cibiru,Bandung40617*E-mail:[email protected]

ABSTRACT

Cancerisadiseasethatinvolvesgeneticfactorsinitspathogenesisprocess.Theincreaseofcellsurvivalasa resultofgeneticchanges thatpreventapoptosis suchasBcl2activation (B-celllymphoma-2)willcausethetumortogrow.TheoverexpressionofBcl2 insmall lungcancershould be inhibited. This study aims to screen natural product that can inhibit Bcl2overexpression in lung cancer using pharmacophore and molecular docking-based virtualscreeningtoZINCNaturalProductdatabase.Thevalidationofpharmacophore-basedvirtualscreening to the three features pharmacophore model (2 hydrophobic interactions and 1HydrogenBondDonor)showedthattheAUC,EF,Se,Sp,ACC,andGHvalueswere0.57,3.8,0.101,0.957,0.936and0.149respectively.WhilethevalidationofmoleculardockingbasedvirtualscreeningshowedthattheRMSDvaluesofVinaWizardandAutoDockWizardwere1.3Åand 1.9Å respectively. Pharmacophoremodel virtual screening obtained 6,615 compoundsfollowedbymoleculardockingbasedvirtualscreeningusingVinaandAutodockwizardsthatfinally obtained 255 compounds that had lower ΔG dan Ki of the natural ligand. It wasconcludedthatthevirtualscreeningobtained255potentialanti-lungcancerdrugcandidates.Keywords:B-celllympoma2inhibitor,moleculardocking,pharmacophoremodeling,virtualscreening


76


POTENTIALETHANOLICEXTRACTOFPASAKBUMI(Eurycomalongifolia)NANOPARTICLESASANAGENTOFINCREASINGARTEMISININACTIVITYONPlasmodiumfalciparum

SriWigati*,DiahTriUtami,IndriMaharini

DepartmentofPharmacy,FacultyofScienceandTechnology,UniversitasJambi,Indonesia


ABSTRACT

Artemisinin Combination Therapy (ACT) is an effective therapeutic strategy for treatingfalciparummalaria.However,currentlyPlasmodiumfalciparumhasexperiencedresistancetoACT.Oneeffort that canbedeveloped tominimize resistantdrugs inmalaria therapy isbydevelopingadrugdeliverysystemformulaintheformofnanoparticles.Naturalmaterialsthathavethepotentialtobedevelopedascombinationagentsformalariatherapywithartemisininare pasakbumi (Eurycoma longifolia Jack.). Pasak bumi contains active compoundsderivedfromeurycomanoneasanantimalarialagent.Withthenanoparticlesystem,itisexpectedthatextracts containing active compounds of eurycomanone derivatives can reach intracellularparasites,sothattheeffectivenessofthetreatmenttobecombinedwithartemisininisgreater.Theexperimentbeginswithpreparationnanoparticlesofpasakbumiethanolicextract(NEEPB)waspreparedby ionicgelationmethod.TheantimalarialactivityofNEEPB,artemisinin,andtheircombinationwasevaluatedmicroscopicallybycountingthepercentageofP.falciparumafter24hoursincubationtimetodeterminedparasitemiarates(IC50)andCIvalues.NEEPBandartemisininshowedantimalarialactivityonP.falciparumwithIC5032,3%and5,39x10-5µg/mL,respectively.BasedonCIvalues,combinationconcentration1/2,3/8,1/4and1/8IC50NEEPBwith1/2,3/8,1/4and1/8IC50artemisininshowedstrongsynergistic-synergisticeffect(CI0,1-0,7).TheseresultsprovideevidencesupportingthedevelopmentofNEEPBasartemisininco-therapeutic antimalarial agent, by enhancing its toxicity effect on P. falciparum in vitro.Therefore,furtherstudyonitsmolecularmechanismneedstobeconducted.Keywords:antimalarial,NEEPB,Plasmodiumfalciparum


77


ACTIVECOMPOUNDSFROMAmomumcompactumSol.ExMaton

DedenIndraDinata1*,UnangSupratman2,3,RaniMaharani2,3,FauzanZeinMuttaqien1

1BandungSchoolofPharmacy,Jl.SoekarnoHatta754,Bandung40615,Indonesia

21DepartmentofChemistry,FacultyofMathematicsandNaturalSciences,UniversitasPadjadjaran,Jatinangor45363,Indonesia.

3CentralLaboratory,UniversitasPadjadjaran,Jatinangor45363,Indonesia*E-mail:[email protected]

ABSTRACT

DiscoveryofactivecompoundsfromIndonesianendogenousplantsplayanimportantroleintheanticancer drugs discovery. This research aim to obtain active compounds from the roots ofAmomumcompactumSol.ExMaton.IsolationmethodwascarriedoutbyusingMTTAssayagaintsbreastcancercellline.TheextractsshowedcytotoxicactivityagainstMCF-7breastcancercellswithIC50valuesof0.37μg/mL(ethanolextract),0.22μg/mL(n-hexanefraction)and0.40μg/mL(ethylacetatefraction),respectively.Fromthen-hexanefractionobtainedtwophenylpropanoidtypesofcompoundonthebasisofspectroscopicdatainterpretation.Thisresultsleadtothefindingofnewsourceofplantsaspotentialanticanceragents.Keywords:AmomumcompactumSol.ExMaton¸activecompounds,cytotoxicactivity


78


ALPHAGLUCOSIDASEENZYMEINHIBITORYACTIVITYOFOKRA(Abelmoschusesculentus(L.)Moench)FRUIT

WidhyaAligita*,AriYuniarto,Dahlia

DepartmentofPharmacology,BandungSchoolofPharmacy,Jl.SoekarnoHatta

754Bandung*E-mail:[email protected]

ABSTRACT

Okra fruit isone speciesof Indonesianplants thatareempiricallyusedasantidiabetic. Thisstudywasconductedtodeterminetheinhibitoryactivityofα-glucosidaseenzymeofokrafruitusing in vitro and in vivo method. The in vivo inhibition activity of α-glucosidase enzymesperformed on sugar-inducedmale swisswebstermice induced at dose of 3 g / kgBW. Theanimalwas divided into seven groups, thatwere negative control, positive, standard drug,waterextractokrafruitdose25,50,100,and200mg/kgBWgroups.Thedatawereanalyzedbyone-wayANOVAstatisticswithpost-hocLSD.Theresultsshowedthattheextractofokrafruitwater at all doses had the activity to decrease post-prandial blood glucose level. Thisdecreasewassignificantlydifferentcomparedtothestandarddruggroup.However,theinvitroresult of inhibition of α-glucosidase showed IC50 1533.742 μg /ml for the okra fruit waterextractwhiletheacarbosewas40.928μg/ml.Inconclusiontheokrafruitwaterextracthadnoactivityagainst inhibitionofα-glucosidasebuthad theactivity todecreasepost-prandialbloodglucoselevel.Keywords:okrafruit,Abelmoschusesculentus(L.)Moench,α-glucosidaseenzyme


79


IN-SILICOANALYSISANDHOMOLOGYMODELINGOFNS1ANTIGENINDONESIAONDIAGNOSTIC

SENSITIVITYTESTOFDENGUE

DewiAstriany1,4,*,HermanSusanto1,GinaTazkyaRahmaniah1,AriHardianto2,MuhammadYusuf2,3,AdeRizqi3,TotoSubroto2,3

1DepartmentofPharmacy,IndonesianSchoolofPharmacy,SoekarnoHattaStreet,Bandung402662DepartmentofChemistry,FacultyofMathematicsandNaturalSciences,PadjadjaranUniversity,

Bandung-SumedangStreetKm.21,Sumedang,453633ResearchCenterofMolecularBiotechnologyandBioinformatics,PadjadjaranUniversity,

SingaperbangsaStreet,Bandung,401334DoctoralProgrammeinDepartmentofChemistry,FacultyofMathematicsandNaturalSciences,

PadjadjaranUniversity,Bandung-SumedangStreetKm.21,Sumedang,45363


ABSTRACTDengueisamosquito-bornediseasecausedbydenguevirus(DENV)-1,DENV-2,DENV-3,andDENV-4 infection which in recent years has become a global health threat especially in tropical andsubtropicalcountries.Thenon-structuralglycoprotein-1(NS1)antigenisoneofthedeviceoptioninlaboratorydiagnostictest,thatcanbeusedtodetecteitherprimaryorsecondaryinfectionsintheearliest stages2. Nowadays, the sensitivity of the kit currently diagnosed with DHF duringsurveillanceinIndonesiatobelessthan70%1.Inthisstudy,48structuresoflocallyNS1genesofDENV1-4isolatesfromIndonesiaanalyzedtoobtainthebestmodelusingbioinformaticsapproach.Homology modeling was carried out using Modeller 9.19 through templates that have a highhomologylevelwiththeaminoacidsequenceNS1antigen.UsingBLASTandPyre2,weobtainedthebesttemplatewithPDBID4O6B_A.ThebestmodelofDENV-1,DENV-2,DENV-3,andDENV-4wereNS1/ID/DENV1-20withRamachandranplotvalue91.3%,NS1/ID/DENV-2-2withRamachandranplotvalue 90.5%, NS1/ID/DENV3-6 with Ramachandran plot value 90.6% and NS1/ID/DENV4-1 withRamachandranplotvalue90.2%.Theresultsshowedthatthedifferencesofstructures,aminoacidresidues, epitopes from templates and each serotype are valuable tools for designing antibodyfragmenttoenhancethesensitivityoflaboratorydiagnostictest.Keywords:denguevirus,NS1antigen,homologymodeling.


80


ISOLATIONOFANTIBACTERIALCOMPOUNDFROMTHELEAVESOFMIKANIAMICRANTHAH.B.K

LiliAndriani*,SantiPerawati,PutriPratiwi

ProdiFarmasi,StikesHarapanIbuJambi,Jl.TarmiziKadirNo.71PakuanBaru,Jambi36132*E-mail:[email protected]

ABSTRACT

Mikaniamicrantha isoneoftheherbsthatusedastraditionalmedicine,suchasSukuAnakDalaminJambiwhichuseMikaniamicranthaleavestotreatwounds.Basedthisfact,Mikaniamicrantha leaves have antibacterial potency. The purpose of this researchwas to analyzesecondary metabolites from the leaves of the Mikania micrantha was potential asantibacterials. Researchmethods startedwith the collection of the sample, extractionwithethanol 70%, isolation, test of antibacterial activity, and characterization of antibacterialcompounds using spectrophotometry UV and FTIR. The results of the screening of ethanolextracts of leaves of phytochemicals Mikania micrantha contain alkaloid, polyphenols,flavonoids,saponins,andsteroids.Testingofantibacterialactivityofextracts, indicatingthefractionofethylacetate(Mm-II),subfraction(Mm-II-D),andisolates(Mm-II-D4)showedthegreatestactivityagainstthebacteriaS.aureusandE.coli.Theseparationofcompoundwasdone using the extraction method of liquid-liquid (ECC), column chromatography andpreparative thin layer chromatography. Purification of isolates performed with two-dimensional thin-layer chromatography. Characterization of the active antibacterial isolate(Mm-II-D4)showedUVspectraindicateamaximumwavelengthof234nm,andFTIRanalysisindicatedtheexistenceofC=Caromatics,C=O,andOH.Basedontheresultsofthespectrum,theallengedcompoundofantibacterialactiveisolatesfromleavesofMikaniamicranthawasm-methoxybenzoicacid.Keywords:Mikaniamicrantha,antibacterial


81


COMPARISONOFANTIBODYAFFINITYENERGYOFCHK265WITHCHK152ASARAPIDDIAGNOSTICCOMPONENTOFANTICHIKUNGUNYAVIRUS

KorryNovitriani1,4.,AdeRizki3,MuhammadYusuf2,3,*,ShabarniGaffar2,TotoSubroto2,3

1DepartementofMedicalLaboratoryTechnology,SekolahTinggiIlmuKesehatanBaktiTunasHusada,Cilolohanstreet,Indonesia,46115.

2DepartementofChemistry,FacultyofMathematicsandNaturalSciences,UniversitasPadjadjaran,BandungSumedangKM.21,Indonesia,45363.

3ResearchCenterofMolecularBiotechnologyandBioinformatic,UniversitasPadjadjaran,Singaperbangsastreet,Indonesia,40133.

4DoctoralPrograminDepartementofChemistry,FacultyofMathematicsandNaturalSciences,UniversitasPadjadjaran,BandungSumedangKM.21,Indonesia,45363.


ABSTRACT

Identifiedantibodiescanneutralizeandprotect thebody fromalfavirus infections includingchikungunya.ThereareseveralantibodiesthatcanneutralizechikungunyaincludingCHK152which neutralizes virus by inhibiting fusion with stabilizing the surface area of the virus,hindering the exposure of the fusion-loop, likely neutralizing infection by blocking fusion.OtherwiseCHK265inhibitsthelifecycleofthevirusinawayneutralizeinfectionbyblockingentry at a post-attachment pre-fusion step. The low energy of binding between antigen-antibodieswillstabilizetheinteractionofboth.Thisworkaimstofindthebestaffinityenergyforantibodiestobeusedintherapiddiagnosticcomponentofanti-chikungunyavirus(CHIKV).ThebindingenergieswerecalculatedusingFireDockanefficientmethodfortherefinementand rescoring of rigid-body docking solutions, through rearrangement of the interfacesidechainsandadjustmentoftherelativeorientationofthemolecules.TheresultsofthisstudyobtainedenergyscoresfromCHK265andCKH152are-2.07and-18.74,indicatethatCHK265antibodyaffinityenergyishigherthanCHK152.ItcanbeconcludedthatthebestaffinityenergyforCHIKVdiagnosticcomponentisCHK152.Keywords:energyaffinity,antibody,CHIKV,FireDock


82


ANTIAGINGPOTENTIALOFMORINGAEXTRACTS

NunukAriesNurulita1,2*,ElzaSundhani1,2,AnjarMahardianKusuma1,DesyAmaliaGhalyati1,

IsnaeniWardani1,FifiRahmawati1

1)FakultasFarmasi,UniversitasMuhammadiyahPurwokerto,CentralJava,Indonesia

2)CancerandStemCellResearchCenter(CSCR),UniversitasMuhammadiyahPurwokerto,CentralJava,Indonesia


ABSTRACT

Theexposureofreactiveoxygenspecies(ROS)canstimulateskinaging.ThemaintenanceofROS level and antioxidant homeostasis are important strategies for preventing and/orpostponingskinagingprocess. ThepreliminarystudyofMoringaextractexhibitedthehighpotency of antioxidant so that it could be developed as anti-aging agent. In this study,wepreparedtwotypesofMoringaethanolicextracts (leavesandstem).Thetotalphenolicandflavonoid compoundswere analyzed andwe observed high content of both compounds inMoringaextracts.Stemextractcontainhigherphenolicandflavonoidcomparedwith leavesextract.Incorrelatedwiththis,thestemextractresultedanticollagenaseactivitystrongerthanthe leaves extract. The leaves and stemMoringa extract showed no cytotoxicity effect onnormal fibroblast cells, NIH3T3, with IC50 values 1526 and 2040 ppm, respectively. BothMoringaextracts(50–250µg/mL)hadstrongeffectsoncells-basedsystems,includingH2O2–inducedoxidative stress inNIH3T3 throughprotected the inductionof celldamageandcelldeath.Theprotectiveeffectwasdeterminedastheincreasingofcellsviabilityoftreatedcellscomparedtountreatedones.Theresultsshowedstrongereffectofbothextractscomparedascorbicacidasstandardantioxidantatthisstudy.TheextractofleavesandstemofMoringareverseH2O2-inducedcelldamagewiththree-hour-highconcentrationexposure.Thereversaleffect of both extracts higher than ascorbic acid. The low concentration of both extractsdemonstrated better result compare with higher ones. Moringa extracts demonstratedcytoprotective effect and cell recovery of damaged cells caused by free radicals induction.Therefore,weconcludedthattheMoringaextractcanbeapotentialanti-agingagent.Keywords:Moringaextracts,anti-aging,H2O2-induction,cellrecovery,cytoprotective,NIH3T3


83


CYCLODEXTRINEGLUCANOTRANSFERASEPRODUCTIONBYLOCALBACTERIAFROMCASSAVASOIL

DesiSagita1,*,Nurantriana1,SitiHamidatulAliyah1,YuniAndriani1,GeniaKarolina1,NiraVionita1,BarmiHartesi1

1ProdiFarmasi,SekolahTinggiIlmuKesehatanHarapanIbuJambiJl.TarmidziKadir71PakuanBaruJambiCode36121


ABSTRACT

GTaseisextracellularenzymesofmicroorganismsthathavebeenusedtoconvertstarchintocyclodextrin(CD)viacyclizationreaction.MajorproducersofCGTasebelongtoBacillusandfewofthemarearchaebacteria.ScreeningactivitiesofCGTasefromisolateinvolvestheuseofsolidHorikoshimediacontainingphenolphthaleinandmethylorangeasindicatorsthatwasdetectedby formation of clearance zone around the bacterial colony. The phenotypic isolate wascharacterizedbygramstaining,catalaseactivity,producingsporesandmotility.Thegenotypeisolatewascharacterizedby16SrRNA,genesequencesobtainedandalignedwiththeClustalWProgramme.CGTaseproductionfromisolatewasproducedbybatchcultivation.β-CGTaseactivity of isolate was assayed by the phenolphthalein method based on complexation ofsupernatants with phenolphthalein and measured at 550 nm. The reduction in the colourintensityofphenolphthalein after complexationwasdefinedas theamountofCGTase thatformed1µgofβ-CDmin-1.TheoptimumincubationperiodandtemperatureofisolategrowthforCGTaseproductionwasalsocharacterized.TheresulthasbeenshowedthenewalkaliphilicBacillus producer of cyclodextrin glucanotransferasewas isolated from local Cassava soil atJambiandwasnamedbyBV-3.Itwasgram-positive,motile,catalase,andendosporeforming.According to 16S rRNA, BV-3 was clustered into Bacillus alkalophilus species. BV-3 wasproducedcrudeenzymesβ-CGTaseactivityafter48hincubationat30°Cinrange970,4µg/mLunderalkalicondition(pH10).Keywords: Alkalophilus bacillus, 16S rRNA gene sequence, Cyclodextrins, CyclodextrinGlucanotransferase


84


SEDATIVEEFFECTOFCITRONELLA(Cymbopogonnardus(L.)Rendle)TOWARDSMALEMICE(Musmusculus)

Yulianita*,E.MulyatiEffendi,EkasMaraFirdayani

DepartmentofPharmacyandBiology,FacultyofMIPA–PakuanUniversity,

Jl.PakuanPo.Box452*E-mail:[email protected]

ABSTRACT

Hypnoticsaresubstanceswhichincertaindoseismeantforescalatingpsychologicaldesiretosleepandreduceandcausingsleep.Theresearchwasaimedtodeterminingtheinfluenceandtheeffectivedoseofcitronellagrassassedativesubstanceappliedonmalemice.Animalsusedonthetestwereamountedto25malemicewhichweredividedinto5groups,eachofthemconsistedof5mice.Who’sgiventhecitronellagrassextractwereDoseGroupI(12mg/20gBW),DoseGroupII(24mg/20gBW),andDoseGroupIII(48mg/20gBW),bymeansofNegativeControlGroup(-)0,5mLofdistilledwater,andPositiveGroupControl(+)intermsof0.013mL/20gBWDiazepam.The resultsof the researchshowthat theconferralof citronellagrassessence ispositivelypotentialasasedativesubstanceonthemice.Thus,DoseIII(48mg/20gBW)becomesthemosteffectivedosewith themiceonsetof fallingrecorded46.7seconds.Theconferraldurationofcitronellagrassessencetowardssedativeeffectonmalemicewiththedose(III)of48mg/20gBWisthemosteffectivedoseduetotherelativesimilaritywithdiazepamaspositivecontrol,observedfromtheaveragedurationbeingclosetodiazepamaspositivecontrolwithinthe average onset amounted to 4.46 seconds, while diazepam itself is amounted to 1.38seconds.Keywords:CitronellaGrass,Sedativum,Rotarod


85


Pharmacokineticinteractionsbetweenwarfarinandjavaneseturmeric(CurcumaxanthorrhizaRoxb)extractinrats

TaofikRusdiana1,*,NoriscaAlizaPuteriana1,YanniDMardhiani2,DolihGozali1,Takuya

Araki3,KoujiroYamamoto3

1DepartmentofPharmaceutics,FacultyofPharmacy,UniversitasPadjadjaran,Jatinangor,Indonesia

2ResearchGroupofPharmaceutics,BandungSchoolofPharmacy,Bandung,Indonesia.3DepartmentofPharmacy,GraduateSchoolofMedicine,GunmaUniversity,Maebashi,Japan


ABSTRACT

Warfarin (WF) is still the drug of choice for anticoagulant therapy in the treatment ofthromboembolism-related diseases such as atrial fibrillation, deep vein thrombosis andpulmonary thromboembolism. However, the use of warfarin requires an extra carefullymonitoringbecauseofthehighvariabilityofindividualpatientresponseasaconsequentlyofanarrowtherapeuticindexdrugandisstronglyinfluencedbygeneticpolymorphismsrelatedtoitsmetabolismandmoleculartargets.Manyfactorscancausesignificantchangestowarfarinresponses in an individual patient, including age, sex, body weight and body surface area,smokinghabits,diet,geneticfactorsandinteractionswithdrugs,food,herbsorsupplements.Oneof theherbs that isoftenconsumedby thepeople isCurcumaxanthorrhizaRoxb (CX),whichisoftenfoundindrinks,foodandherbalmedicine.Thisstudyinvestigatedtheeffectofconcomitant administration of CX and WF to determine whether or not a significantpharmacokineticalteration.ThestudywasconductedbygivingWFandCXinthreegroupsofrats.GroupI(n=6)wasgivenwarfarinalone,groupII(n=6)wasgivenWFandCX-1(normaldose)andgroupIIIweregivenWFandCX-2(highdose),thenbloodsamplingwascarriedoutineachmouseat15minutes,30minutes,45minutes,1,2,4,8,12,24,48and72hoursandWFlevelsineachsampleweredeterminedandpharmacokineticparameterswerecalculated.TheresultsshowedthatallofPKparametersofR-WFinrattreatedbytheCX-1groupwerenotdifferentfromthecontrolgroup.WhiletheAUC,ClandVdofS-WF intheCX-1groupweredifferentfromthoseofthecontrol.AlmostallPKparametersofS-WFandR-WFinrattreatedbytheCX-2groupwerewidelydifferentfromthoseofthecontrolgroup.TheeliminationrateofS-WFandR-WFintheCX-2groupseemedslowerthatofthecontrolgroup.TheAUCofSandR-WFintheCX-2washigherthanthatofthecontrolandtheCLandVdofS-WFintheCX-2were lowerthanthat thecontrolgroup.Theconclusion is that theCurucumaxanthorrhizaeextractdidnotsignificantlyalterthepharmacokineticsparameterofwarfarinatanormaldosebutsignificantlyalteredatahighdoseadministration.Keywords:Warfarin,Curcumaxanthorrhizae,pharmacokineticsinteractions,AUC,half-life.


86


ANALYSISOFLEADLEVELSONMASCARACOSMETICSFROMKIARACONDONGMARKETUSINGATOMABSORPTIONSPECTROPHOTOMETRYMETHOD

FentiFatmawati*,AiyiAsnawi,SigitErawan

SekolahTinggiFarmasiBandung,Bandung,WestJava,Indonesia


ABSTRACT

Mascaraisaneyelashmakeuptooltogivetheimpressionofthickandlongeyelashes.Mascaracanbepurchasedfreelyonthemarket.Someofthesecosmeticproductsarenotregisteredsotheyruntheriskofbeingcontaminatedbyheavymetalsthatcanharmthehumanbody.Atomicabsorptionspectrophotometrymethodcanbeused inthisstudybecause itcananalyzetheconcentrationofheavymetalsinthesampleaccuratelyevenatverylowconcentrations.Thelimitof leadcontaminationincosmetics,accordingtoBPOMRepublicof Indonesia,mustbelessthan20ppm.ThepurposeofthisstudywastodeterminePblevelsinmascaracirculatingintheKiaracondongmarketbyatomicabsorptionspectrophotometryat283nmwavelength.Theresultsoftheanalysiscarriedouton6samplesdividedintothreeregisteredsamplesand3unregisteredsamples.Twoofthethreedetectedmascarasamplescontainedlead(MTR1andMTR2) which each contained lead of 7.1729 ppm and 149.77781 ppm. The MTR2 samplecontainsleadthatexceedstheallowedthreshold.Oftheunregisteredsamples,twoofthemweredetectedtocontainlead(MTTR1andMTTR2),eachofwhichcontainedheavymetalsof15.6118ppmand9.2827ppm.AtomicabsorptionspectrophotometrymethodcanbeusedtoanalyzethelevelsofPbinmascaracosmeticwithsatisfactoryresults.Keywords:AAS,Cosmetics,Lead,Mascara.


87


STIGMASTEROLOFETHYLACETATEEXTRACTFROMSTEMBARKOFMelochiaumbellata(HOUTT)STAPFVAR.VISENIA(MALVACEAE)ANDDENGUEANTIVIRALACTIVITY

NunukH.Soekamto*,Firdaus,andFauziahAhmad

Departmentofchemistry,FacultyofMathematicsandNaturalSciencesHasanuddin

University,JlPerintisKemerdekaanKm10Makassar90245*E-mail:[email protected]

ABSTRACT

M.umbelatta(Houtt.)Stapfvar.Vicenia(Houtt.)(Malvaceae)islocallyknownasPaliasa.theseplantsuseastraditionalmedicines.StigmasterolhavebeenisolatedofethylacetateextractofstembarkM.umbelatta (Houtt.)Stapfvar.Vicenia (Houtt.)and itsactivity tests fordengueantiviralcausingdenguehemorrhagicfever(DHF).Themethodofthisresearchwasextraction,fractionation,purificationandactivitytestagainstdenguevirus.Thestructurewasestablishedon thebasis of spectral analysis. Stigmasterol showedactive against dengue viruswith IC50valuesof9.1070μg/mL.Keywords:M.umbelatta(Houtt.)Stapfvar.Vicenia(Houtt.);Malvaceae;stigmasterol;denguevirus.


88


COMPARISONOFBULKANDPRECIPITATIONPOLYMERIZATIONMETHODOFSYNTHESISMOLECULARIMPRINTEDSOLIDPHASEEXTRACTIONFORATENOLOLUSING

METHACRYLICACID

RimadaniPratiwi,SandraMegantara,DriyantiRahayu,IndraswariPitaloka,AliyaNurHasanah*

DepartmentofPharmaceuticalAnalysisandMedicinalChemistry,FacultyofPharmacy,

UniversitasPadjadjaran,Jatinangor45363,Indonesia*E-mail:[email protected]

ABSTRACT

Atenololisoneofbeta-blockerareprohibitedasdopingbasedonWorldAnti-DopingAgency(WADA)1.Thepurposeofthisstudywastothesynthesisofmolecularimprintedpolymer(MIP)forextractionofatenololfromthesample.Thisresearchcomparedthetwoofthemethod,bulk andprecipitationpolymerization. TheMIPwas successfully prepared frommethacrylicacidasafunctionalmonomer,ethyleneglycoldimethacrylateasacrosslinker,benzoylperoxideasaninitiator,butanolasaporogenicsolventwithatenololasatemplatemolecule.Theresultshowed that thebulkpolymerizationmethodproduces sorbents thathavegoodadsorptioncapacityandsmallparticlecomparetotheprecipitationpolymerization.Bothmethodswereselectiveforatenolol.Generally,theMIPsolidphaseextractionisanalternativemethodforextractionatenololfromthesample.Keyword:Atenolol,MolecularImprintedPolymer,SolidPhaseExtraction


89


PRELIMINARYASSESSMENTONCORRELATIONOFFASTINGAND2-HPOSTPRANDIALGLUCOSELEVELCHANGEWITHQUALITYOFLIFEOFTYPE2DIABETESMELLITUSPATIENT

USINGINDONESIANEQ-5D-5LVALUESET

Iskandar,Deni(1);Sutrisno,Entris(1);Maesaroh,Nono(1)

(1)BandungSchoolofPharmacy,Jl.SoekarnoHattaNo.754,Bandung40617*E-mail:[email protected]

ABSTRACT

Theprevalenceofdiabetes in Indonesiasince1980to2014 increases,poormanagementofdiabetes can lead to micro or macro-vascular complications and decrease quality of life.StandardizedmeasureofhealthstatusoftheEuropeanQualityofLife5Dimension5Level(EQ-5D-5L)instrumentisavailablewithIndonesianvaluessetfortheevaluationofhealtheconomicsandhealth-relatedqualityof life in Indonesia.Theobjectiveof this studywas toassess therelationship betweenblood glucose level change and quality of life of patientswith type 2diabetesmellitus.Thiswasananalyticobservationalstudyincitygovernmentpublichospitalfrom March–May 2018 using cross-sectional design, with 4 months (January–April 2018)retrospectivesecondarydataofmedicalandlaboratorytestrecords,whereasprimarydataofhumanisticoutcomeobtainedin5thmonth(May2018)usingofficialIndonesiantranslationofEQ-5D-5L questionnaires from EuroQol Group, therefore no further validity or reliabilityanalysiswasperformed.The subjectswere81adultswith type2diabetesmellituspatientsbasedon ICDXCodeE.11.9,havingnot lessthan4visits inMay2018,excludingthosewhorefusedtoparticipate.Datawereanalyzedwithunivariateandbivariateanalysistodeterminethecorrelationofbloodglucoselevelchangeandqualityoflife.Resultshowedthatmajorityofrespondentwerefemale,54(66.67%);withtheagegroupof60–65years,22(27.16%);havingfinished senior high school, 25 (30.86%); and only few of them is working, 17 (20.99%). 4months’averageandaveragedecrementfrom4thmonthagainstpreviousmonthforFastingGlucosewere155.8±55.17mg/dLand1.70±45.72mg/dL,respectively;asfor2-hPostprandialGlucosewere194.17±70,72mg/dLand13.11±61.86mg/dL,respectively.Averagequalityoflifewas71.111±13.181QALYs.BivariateanalysisshowednosignificantcorrelationbetweenFastingGlucose (r= -0.264,n=43,p=0.087) and2-hPostprandialGlucose (r= -0.171,n=37,p=0.312)changewithqualityoflife.Basedontheanalysis,itcanbeconcludedthatthereisnosignificantcorrelationbetween fastingand2-hpostprandialglucosewith thequalityof lifeofpatientswithtype2diabetesmellitus.Keywords:QualityofLife,EQ-5D-5L,Type2DiabetesMellitus,IndonesiaValueSet


90


QUANTIFICATIONOFISOFLAVONEANDANTHOCYANINCONTENTINTHEBLACKSOYBEANLINES

DadangSumardi1),AdiPancoro1),HusnaNugrahapraja1),RijantiRahajuMaulani1,*,EriMustari1)

andAgungKaruniawan2)

1SchoolofLifeSciencesandTechnologyInstitutTeknologiBandungLabtekXI,SITHBuilding,Jl.Ganesa10,Bandung,Indonesia,40132

2FacultyofAgricultureUniversitasPadjadjaran,JalanRayaBandung-Sumedangkm21JatinangorSumedang,Indonesia,45363*E-mail:[email protected]

ABSTRACT

Blacksoybeancontainshighlevelsofisoflavoneandanthocyaninthatgivebeneficialeffecttothehumanhealth.Isoflavonesarethemostcommonformofphytoestrogen.Anthocyaninhavebeenrecognizedashealth-promotingfunctional food ingredientsduetotheirantioxidantactivity.Theimportanceofthesephytochemicalshaveledtothedevelopmentofblacksoybeanthathavehighlevelsofisoflavoneandanthocyanin.Weaimtoquantifytheisoflavoneandanthocyanincontentintheparentallinesandtheprogenyfromthecrossingscheme.Weusedparentallines(UP106andUP122)andfourgroupofprogeny(F4(106x122)a,andF4(106x122)b,F4(122x106)e,andF4(122x106)f,,)thatplantedinanaugmenteddesign.Theaglyconeformofisoflavonewasmeasuredinblacksoybeanseedusinghigh-performance liquid chromatography. The cyanidin-3-glucoside form of anthocyanin wasmeasured in blacksoybean seed using spectrophotometer. The result of the study showed thatisoflavone levels were inversely proportional to anthocyanin levels in blacksoybean seed.BlacksoybeanlineofUP106,F4(106x122)a,andF4(106x122)b,havetotalisoflavonecontentof0.37mgg-1,0.32mgg-1and0.21mgg-1respectively.BlacksoybeanlineofUP122,F4(122x106)e,andF4(122x106)f,havetotal isoflavonecontentof 0.25mgg-1,0.14mgg-1and0.21mgg-1.TheanthocyanincontentofUP106,F4(106x122)a,andF4(106x122)blinesare3.05mgg-1,6.06mgg-1and4.27mgg-1.Theanthocyanincontentof UP122, F4(122x106)e,and F4(122x106)f linesare4.47mgg-1,10.89mgg-1and8.16mgg-1respectively.BlacksoybeanofUP106ispromisinglinewithhighisoflavonecontentandUP122isthepromisinglinewithhighanthocyanincontent.Keywords:BlackSoybean,Aglycone,Cyanine-3-Glucoside,NutritionalValue,F4Cross


91


TOTALFLAVONOIDSANDANTHOCYANINCONTENTOFPIGMENTEDRICE

RijantiRahajuMaulani,DadangSumardi*,AdiPancoro

SchoolofLifeSciencesandTechnologyInstitutTeknologiBandung,LabtekXI,SITHBuilding,Jl.

Ganesa10,Bandung,Indonesia,40132*E-mail:[email protected]

ABSTRACT

Pigmented rice is currently becoming the popular food due to their functional properties.Pigmentedricenaturallycontainsphytochemicalsthathavebeneficialeffecttohumanhealth,especiallyasantioxidants,suchaspolyphenolsandanthocyaninpigments.InWestJava,thereare local varieties of black, glutinous black and brown rice which can be developed as afunctional food. The objective of this study was to quantify total flavonoids and totalanthocyanins,andtheirantioxidantactivity.Thericeseedwereevaluatedforcolorintensity,theyieldofextract, total flavonoids, totalanthocyanin,andantioxidantactivityusedkineticassays of DPPH. The result showed that the increase in color intensity increased the totalflavonoids,totalanthocyanin,andtotalantioxidantcontent.Blackriceandblackglutinousricehavetotalflavonoidscontent0.19mgg-1and0.15mgg-1,respectively.Thetotalanthocyanincontentofblackriceandblackglutinousricewere0.40mgg-1and0.29mgg-1,respectively.The local variety of black rice and black glutinous rice can be developed as an alternativefunctionalfood.Keywords:blackrice,brownrice,blackglutinousrice,anthocyanin,flavonoids,functionalfood


92


FORMULATIONANDCHARACTERIZATIONINMICROENCAPSULATIONOFPETAI(ParkiaspeciossaHassk.)LEAFEXTRACTS

Buanasari1,*,YithroSerang1,SiswoSumardiono2,andBambangPramudono2

1AkademiFarmasiNusaputera,Semarang,Indonesia,50162;[email protected],FacultyofEngineering,DiponegoroUniversity,Semarang,

Indonesia,50275*E-mail:[email protected]

ABSTRACT

ParkiaspeciosaHasskorpetaiisatraditionalmedicinalplantwithstrongantioxidantanditcanbeusedforthetreatmentofhypertension,antiangiogenic,diabetes,antiulcerandheadaches.Petaileafextract(PLE)havebeenreportedhavingantioxidantactivityandhasthepotentialasanaturalantidiabetic.DespitethemanyadvantagesofPLE,theirodorandsensitivitytostorageconditionsisaproblemhere.Thepurposeofthisstudywastodeterminetheeffectivenessofpetai leaf extract in encapsulated form. This study began by extracting petai leaves fromIndonesiausinganultrasonic-assistedextractionmethod.Toimprovethestabilityandsmothertheirodor,PLEencapsulatedbyaspray-dryermethodusingcoatingmaterialincludingarabicgum(GA),Maltodextrin(MD)andvariationsinPLEconcentrationinencapsulationformula(1;2.5and5%w).Encapsulationefficiencyandantioxidantcapacityweredeterminedandwerefoundtobeintherangeof24.86-38.63%,and56.33-86.22%.Theresultsoftheresearchgavetheformulawhichhadthehighestyieldandantioxidantcapacitywas75%arabicgum(GA),25%maltodextrin (MD) and 5% PLEwith encapsulation efficiency and antioxidant capacityvalue were 38.63% and 86.22%. Based on the results of research we found that PLEmicroparticlesareabletocoverodorsandhasapotentialasanaturalantioxidants.Keywords:antioxidantcapacity,microencapsulation,petaileaves,spray-dryer.


93


SYNTHESISANDMUCOADHESIVESTUDYOFNANOPARTICLESBASEDONHYDROXYPROPYLCELLULOSE-CYSTEAMINEASANOVELNANOMATERIAL

DeniRahmat,*StellaSalim,DianRatihLaksmitawati,LiliekNurhidayati

FacultyofPharmacy,PancasilaUniversity,Srengsengsawah,Jagakarsa,JakartaSelatan12640


ABSTRACT

Hydroxypropyl cellulose (HPC)-cysteamine is a novel cationic thiomer generated bymodification of HPC according to the synthesis of hydroxyethyl cellulose (HEC)-cysteamine.HEC-cysteamineshowsbettermucoadhesiveandpermeationenhancingpropertiescomparedtounmodifiedHEC.Inaddition,nanoparticlesbasedonHEC-cysteaminecanbeformedbyionicgelationmethodduetoitscationiccharges.TheaimofthisstudywastopreparenanoparticlesbasedonHPC-cysteaminewithmucoadhesiveproperties.ThesynthesisofHPC-cysteaminewascarriedoutbyoxidationandreductiveaminationreaction.ThepreparationofnanoparticlesbasedonHPC-cysteaminewasperformedusingsodiumtripolyphosphateandsodiumalginateascrosslinkersandthenanoparticleswerethenevaluatedfortheirmucoadhesivepropertieswith fluorescein diacetate (FDA) as a marker. FDA in the solution was attached on thenanoparticlessuspensionbyphysicaladsorptionwhentheyweremixedandshockwithintheincubationtime.50mgofthenanoparticleswerespreadonthesurfaceofporcinemucosaandthephosphatebufferflowedonthemucosa.Theamountofthenanoparticlesonthemucosawas determined by the concentration of FDA based on fluorescence intensity. The resultsshowedthatsodiumalginateasacross linkerproducedthenanoparticleswith76.47nm inparticle size and -24.2 mV in zeta potential. Moreover, the result of mucoadhesive studydemonstratedthattheamountofnanoparticlesattachedonporcinemucosawas3.81%after3hoursofexperimentwhile FDAalonewas found in insignificantamount.Accordingly, thenanoparticles could have a potential to prolong the residence time of the entrapped drugleadingtotheimprovedbioavailability.Keywords:HydroxyPropylCellulose-cysteaminenanoparticles,ionicgelation,mucoahesiveproperties.


94


SYNTHESISANDANTIBACTERIALACTIVITYTESTSOFCURCUMINANALOGUESUSINGBROMOFUNCTIONALGROUP

IsmiRahmawati1,2,*,MarliaSinggih1,DaryonoHadiTjahjono1

1SchoolofPharmacy,BandungInstituteofTechnology,JlGanesha10,Bandung401322FacultyofPharmacy,UniversitasSetiaBudi,JlLetjendSutoyo,Surakarta,Indonesia


ABSTRACT

Curcuminhasabroad-spectrumantibacterialactivity.GuangLiangetal. (2009)provedthatcurcuminanalogueswithβ-monoketonhadabetterpharmacokineticprofilethancurcumin.Thesubstitutionofthehalogenfunctionalgrouptophenolicincreasedantisepticactivity.Theaim of this study was to synthesize curcumin analogues with bromo functional groups toincrease antibacterial activity. Synthesis method with aldol condensation between ketonestartingmaterial(cyclohexanone,cyclopentanoneandacetone)with5-Bromo-2-furaldehydeusing 30% NaOH for catalyst. Synthesized curcumin analogue compounds were bymeltingpoint,TLC,GC-Massspectrometers,FTIRspectrophotometer;NMR1Hspectrophotometer,and13CNMR spectrophotometer. Pure compounds antibacterial activity were tested againstbacteria Gram-positive (Bacillus subtillis, Streptococcus mutans ATCC 2517, StapylococcusaureusATCC25923)andGram-negative(EscherichiacoliATCC25922,SalmonellatyphiATCC13311,PseudomonasaeruginosaATCC27853,KlebsiellapneumoniaATCC10031)usingdiscdiffusionmethod.Threecompoundsofcurcuminanaloguesweresuccessfullysynthesizedi.e.2,6-bis (5'-bromo-2'furanyl) cyclohexanone (26ClH); 2,5-bis (5 '-bromo-2furanyl)cyclohexanone(25ClP);and1,5-bis(5'-bromo-2'furanyl)penta-1,4-dien-3-one(15ClA)withtheyieldof73,5%,70,47%and83,47%,respectively.Theresultofantibacterialactivityassessmentshowed that three compounds had inhibition activity. Compound 15BrA had betterantibacterialactivityagainstGrampositive,compound26BrHforGramnegative.Compound25BrPhadabroaderantibacterialactivityagainstbothpositiveandnegativeGrams.Keywords:curcuminanalog,monocarbonyl,halogen,antibacterial.


95


EFFECTOFPEG400,PEG4000ANDPEG6000ASASUPPOSITORIABASEONPARACETAMOLDISSOLUTIONRATE

NurMadusari*,DoddydeQueljoe,AlasenSembiringMilala*

FacultyofPharmacyUniversityofSurabayaJalanRayaKalirungkutSurabaya60293*E-mail:[email protected]

ABSTRACT

Theformofsuppositorypreparationsismadeasanalternativeroutetoavoidhepaticfirstpasseffect. Today paracetamol is considered to be the safest anti-pain substance, also for self-medication.Dissolutionplaysanimportantroleindrugabsorption;therefore,thedissolutionprofile of a drug preparation is very important. Research has been conducted to obtaindissolutionprofilesofparacetamolsuppositorieswiththreekindsofcarriercompositionPEG400,PEG4000andPEG6000,namely70%:15%:15%(formulaI),50%:25%:25%(formulaII)and30%:35%:35%(formulaIII).Assupportingdata,alsotestedthephysicalcharacteristicsofparacetamol suppositorieswhich include organoleptic test, uniformity of weight, hardness,melting timeanduniformityof content.Thedissolution testwas carriedoutusinga type1(rotating basketball) dissolution test HANSONDissolution Tester in a pH 6.8 pH phosphatesolution of 900 ml at 37ºC with a 100-rpm rotation. The average AUC obtained fromparacetamolsuppositoryformulaI,IIandIIIwas4759.72;4521.79and4270.98withanaveragedissolutionefficiencyof79.33%;75.37%and71.18%.ThetestofthedifferenceintheefficiencyofthethirddissolutionofthestudiedformulawascarriedoutbytheVariantAnalysismethod(one-way ANOVA)with the significance level (α) = 0.05 and the results obtainedwere thesignificant differences between the three suppository formulas. From the results of theresearchthathasbeendone,itcanbeconcludedthatthehigherthePEG400concentration,thefasteritdissolves.Keywords:dissolutiontest,paracetamol,suppository,phosphatebufferpH6.8


96


IN-SILICOSTUDIESANDCHARACTERIZATIONOFSHORTLINEARLIPOPEPTIDE-BASEDTRANSFECTIONAGENTPOTENTIALLYCAPABLEFORNUCLEAREXPORT

Tarwadi1,2,*,AlfanD.Arbianto2,SabarPambudi2,HeniRachmawati1,3,RahmanaE.

Kartasasmita1,SukmadjajaAsyarie1,ColinWPouton4

1SchoolofPharmacy,BandungInstituteofTechnology,Jl.Ganesa10Bandung40132,Indonesia.

2CentreforPharmaceuticalandMedicalTechnology-TheAgencyfortheAssessmentandApplicationofTechnology(BPPT),Jl.M.H.Thamrin8Jakarta10340,Indonesia.

3ResearchCentreofNanoSciencesandNanotechnology,BandungInstituteofTechnology,Jl.Ganesa10Bandung40132,Indonesia.

4DepartmentofPharmaceuticalBiologyandPharmaceutics,VictorianCollegeofPharmacy,MonashUniversity,381RoyalParade,Parkville,VIC3052,Australia.


ABSTRACT

Although a transfection agent capable to complex DNA and protect it from enzymaticdegradation,itmightstillcouldnottransfectanon-dividingcellsefficientlyifthetransfectionagent does not have capability in facilitating nuclear export. The availability of transfectionagentcapableincomplexingDNAandinteractingwithshuttleproteinofimportinalphawhichtransport from cytoplasm to cell nucleus is urgently needed. The aimof this research is toexplorethepossibilityofshortlipopeptidemoleculescapablefornuclearexport.Lipopeptidebearing palmytoil chain and positively charge amino acid of lysine has been designed formolecular docking and molecular dynamic studies. Nuclear Localization Sequence (NLS), anative ligandof importinalphaof–PKKKRKV-isused fora referenceof lipopeptide-importinalphainteraction.MarvinSketch18.4.0softwareisusedforstructuralgeometryoptimizationof the lipopeptideproposed.Meanwhile, virtual dockingprocess andmolecular dynamicofimportin alpha (PDB ID: 1EJL) used Autodock Vina and Amber 14 software. Amongst thelipopeptidemolecules being studied, Palmytoil-CKKHH had the highest probability used fortransfectionagentwhichhascapabilityofnuclearexport.Inaddition,thelipopeptidecapablein condensing DNA efficiently, non-toxic to cell targets and protect it from enzymaticdegradation. In summary, lipopeptide of Palmytoil-CKKHH is potentially to be developedfurtherasnon-viralgenedeliverycapablefornuclearexport.Keywords:Amber14,autodockvina,moleculardocking,moleculardynamic,lipopeptide,andtransfectionagent


97


COMPUTATIONALANTIGENICEPITOPEPREDICTIONOFCLINICALINDONESIANDENGUEVIRUSNS1PROTEIN

SabarPambudi1*,DoddyIrawan1,AlfanDanny2,Tarwadi2

1LaboratoryofMolecularBiologyforHealth,CenterforPharmaceuticalandMedicalTechnology,BPPT,Indonesia,15314

2LaboratoryofComputationalandSynthesis,CenterforPharmaceuticalandMedicalTechnology,BPPT,Indonesia,15314


ABSTRACT

ElucidationofhumanB-cellandT-cellepitopesofNon-Structural-1(NS1)proteinarerequiredtoimproveourunderstandingabouttheimmunopathogenesisofDenguevirus(DENV).Inthelastdecades,thevaccinedevelopmentexclusivelydependsonbiochemicalandimmunologicalexperimentwhichareveryexpensive,time-consuming,andlaborious.Withtheaidofinsilicostudy,manyresearchersarenowabletopredicttheepitopeofproteininterestandatthesametimereducingthewetexperiments.Inthisstudy,wepredictedtheT-cellandB-cellepitopesofDENV NS1 consensus sequences originated from Indonesian clinical isolates by exploitingseveralonlineandofflinebioinformaticpredictiontools.WepredictedapotentialCD8+T-cellepitopeatNS1155--163(VEDYGFGIF)withhighbindingaffinity(IC50)scoresrangingbetween63.8nMto183.9nMandaCD4+T-cellepitopeatNS1330--338(WYGMEIRPV)withIC50rangingfrom6.60--9.10nM.Furthermore,wealsopredictedaB-cellsepitopeattheC-terminalofIndonesianDENV NS1325--344 (GEDGCWYGMEIRPVKEKEEN) which matched for both continuous anddiscontinuousantigenicepitope.ThepredictionoftheseT-cellandB-cellepitopesofDENVNS1maypromoteadditionalinformationofDENVimmunopathogenesisandcouldbeusefulintheinitialstepofdevelopmentofanewvaccine,drugdiscoveryanddiagnosticsysteminordertoeliminatedengueinfection.Keywords:dengue;NS1protein;epitope;computational;conserved;consensus


98


HOST-GUESTINTERACTIONSOFALPHAMANGOSTINWITH(α,β,ANDγ)−CYCLODEXTRINS:SEMI-

EMPIRICALQUANTUMMECHANICALMETHODSOFPM6ANDPM7

DoniDermawan1*,NasrulWathoni2,MuchtaridiMuchtaridi31ApothecaryProfession,FacultyofPharmacy,UniversitasPadjadjaran,Sumedang,West

Java,Indonesia453632DepartmentofPharmaceuticsandPharmaceuticalTechnology,FacultyofPharmacy,

UniversitasPadjadjaran,Sumedang,WestJava,Indonesia453633DepartmentofPharmaceuticalAnalysisandMedicinalChemistry,FacultyofPharmacy,

UniversitasPadjadjaran,Sumedang,WestJava,Indonesia45363*E-mail:[email protected]

Thisstudyaimedtoinvestigatethemolecularinteractions,geometricalproperties,encapsulationprocess, and calculated energy of the complexes system between α−mangostin (guest) withα−cyclodextrin, β−cyclodextrin, and γ-cyclodextrin (hosts) in an aqueous environment using thesemi-empiricalquantummechanicalmethodsofPM6andPM7.Moleculardockingsimulationandsemi-empiricalquantummechanicalcalculationsofPM6andPM7wereemployedtoidentifythemolecular interactions between α−mangostin and three types of cyclodextrin. The inclusioncomplex formation energy values of all α−mangostin/cyclodextrin that obtained by the semi-empiricalPM7methodweresignificantly lowerthancomplexationenergyobtainedbythesemi-empiricalPM6method.Theinclusioncomplexofα−mangostin/γ−cyclodextrinisthemostfavorablepathway of inclusion complex formation of α−mangostin with cyclodextrin because it has thehighest negative value of free binding energy (∆G) and complexation energy (ΔE) compared toα−mangostin/α−cyclodextrinandα−mangostin/β−cyclodextrin.Keywords:Alphamangostin,Cyclodextrin,Host-guestinteractions,PM6,PM7


99


SPECTROPHOTOMETRICMETHODFORDETERMINATIONOFBORONCONTENTINFOODWITHPYRIDOXINECOMPLEXFORMATION

AyuShalihat*,BennyPermana,andRahmanaEmranKartasasmita

BandungInstituteofTechnology,Jl.Ganesha10Bandung,WestJava,Indonesia40132


ABSTRACT

Despiteanoffenceagainstthelaw,boronasboricacidorboraxisstillmightbeaddedtofoodproducts.Thepresenceofboroninfoodshouldbeconsideredasasourceofchemicalhazardand hence should be identified and determined. The aim of this researchwas to obtain aspectrophotometricmethodapplicablefordeterminationofboroninfoodproducts.BoronwasreactedwithpyridoxinetoformameasurablecomplexinUVregion.Complexformationwasobserved in pH range of 5.5 to 8.5, in which optimum complex was formed at pH 7 withmaximumabsorbanceat302nm.Calibrationcurveshowedagoodlinearitywithanequationofy=0.0135x+0.3715andacoefficientcorrelationof0.99.Absorbanceofboron-pyridoxinecomplexwasmeasuredat302nmwasstableupto60minutes.Thecomplexformationwasinfluenced by cations including Pb2+, Cd2+, Cu2+, Al3+, Zn2+, Fe3+ andMg2+. For precision andaccuracytest,ricecakewasselectedasmatrixmodelduetocommonboronadditiontothisfoodproduct.Theprecisionofthemethodwascalculatedasarelativestandarddeviation(RSD)wasfoundtobe2.349%;whileitsaccuracywascalculatedasapercentageofrecoveryof160,200and240ppmwere103.78,113.82and100.72%,respectively.Limitofdetection(LOD)andlimitofquantification(LOQ)were0.7652and2.0353ppmrespectively.Basedonoverallresults,itwasconcludedthatthedevelopedmethodcanbeappliedfordeterminationofboroninfood.Keywords:Boron,pyridoxine,spectrophotometry,food,ricecake.


100


ACTIVITYOFPORANGFLOURANDMORINGAEXTRACTTOBLOODGLUCOSEANDLIPIDLEVELSINALLOXANINDUCEDDIABETICMICE

DianRatihLaksmitawati*,SitiFatimah,RistiFathulJannah,YatiSumiyati,UmiMarwati

FacultyofPharmacy,UniversityofPancasila

SrengsengSawah,Jagakarsa,SouthJakarta,12640*E-mail:[email protected]

ABSTRACT

Dietisaplatformofnon-pharmacologicaltherapyindiabeticpatients.Satiatedfoodwithlowglycemicindexisneededtocontrolbloodglucoselevel.TheaimofthestudywastodeterminetheeffectofPorang(AmorphopallusmuelleriBlume)tuberflourandKelor(MoringaoleiferaL)leaf extract to blood glucose, triglyceride and cholesterol levels inAlloxan induceddiabeticmice.Thirty(30)miceweredividedinto6groups,namelynormalhealthygroup(N),diabeticwithouttreatmentgroup(DM0),diabeticwithtreatmentgroupswhichcategorizeasdiabeticwithacarbose(6.5mg/kgBW)/phenofibrate(26mg/kgBW)therapygroup(DMCA/P),diabeticwithporangfeedgroup(DMP),diabeticwithmoringaextractgroup(DME)anddiabeticwithporangfeed-moringaextractgroup(DMPE).Porangfeedwasmadebymixing40%ofstandardfeedand60%ofporangflourtobemoldedintopellets,whilekelorextract(420mg/kgBW)wasadministeredorally.Treatmentwerecarriedout for14daysafterhyperglycemia state.Bloodglucose,triglyceridesandcholesterollevelsweremeasuredatbaseline(beforealloxaninduction), after hyperglycemia state also after 7 and 14 days after treatment. The resultsshowedthatalltreatmentgroupshadasignificantdecreaseinbloodglucose,triglycerideandcholesterolprofiles.Treatmentfor14daysshowedagreaterdecreasecomparedto7days.TheDMPgrouphadthelargestdecreaseinbloodglucoselevelsof67.6%comparedtoothergroups.Thisgroupalsoshowedthelargestdecreaseintriglycerides(59.98%)andcholesterol(33.39%)for14daysofporangfeedingwhereasthedifferencenumberindecreasingtriglyceridesandcholesterolarethesameasDMCphenofibrategroup.Inconclusion,PorangflourandMoringaextracthadtheeffectinreducingbloodglucose,triglycerideandcholesterollevelsinalloxaninduced diabetic mice. Porang feeding group showed the highest reduction effect to allparameters.Thus,theporangtuberisapotentialcarbohydratethathasbeneficialeffectfordiabetes.Keywords:Amorphopallusmuelleri,Moringaoleifera,Diabetesmellitus,glucose,triglycerides,cholesterol.


101


SYNTHESISANDCHARACTERIZATIONOFLIPOPEPTIDE-BASEDTRANSFECTIONAGENTASNON-VIRALGENEDELIVERYVEHICLECANDIDATE

Tarwadi1,4,5,*,SabarPambudi1,AyuN.Sasangka1,AcepR.Wijayadikusumah2,Weiguang

Zang3,DavidC.Jackson3,ColinW.Pouton4,HeniRachmawati5,6,RahmanaE.Kartasasmita5),SukmadjajaAsyarie5

1CentreforPharmaceuticalandMedicalTechnology-TheAgencyfortheAssessmentand

ApplicationofTechnology(BPPT),Jl.M.H.Thamrin8Jakarta10340,Indonesia,2ResearchandDevelopmentDivision,PT.BioFarma,Jl.PasteurNo28Bandung40161,Indonesia,3PeterDohertyInstitute-UniversityofMelbourne,792ElizabethSt,MelbourneVIC3000,Australia,4VictoriaCollegeofPharmacy-MonashUniversity,381RoyalParadeParkville,VIC3052,Australia,5SchoolofPharmacy,BandungInstituteofTechnology,JlGanesa10Bandung40132,Indonesia,6ResearchCentreofNanoSciencesandNanotechnology,BandungInstituteofTechnology,Jl.

Ganesa10Bandung40132,Indonesia.*E-mail:[email protected]

ABSTRACT

Deliveringgeneintocellnucleus,probablyisthehardestchallengeingenetransfer.Thegeneofinteresthastobecompactedandprotectedfromdegradation.Theobjectiveoftheresearchistodevelop a transfection agent which capable to deliver thematerial genetic into the target celleffectively. In this research, lipopeptide of Palmitoyl-CKKHH-YGRKKRRQRRR-PKKKRKV withmolecularweightof3296hasbeensynthesized,characterizedandexploredtobeusedforgenedeliveryvehicle.ItwasrevealedthattransfectionagentoflipopeptidehasabilitytocondenseDNAandprotectitfromenzymaticdegradation.ThecomplexofDNA-transfectionagentwasstableatchargeratioof1.5whereitsparticlesizewas130+0.47nmandzetapotentialwere-24.80+1.04mV.Interestingly,basedontransmissionelectronmicroscopeimageanalysis,thecomplexofDNA-lipopeptidelikelytobeindividualparticlesinceaggregationoflipopeptide-DNAcomplexwasnotobserved.Moreover,thelipopeptidetoxicityonmammaliancellcultureisconsideredlow.Morethan75%cellsofCHO-K1andHepG2growwellinthepresenceofthelipopeptideatchargeratioof5.0.TransfectiononCHO-K1andHepG2cellsbyplasmidencodinggreenfluorescenceproteinwhichwascondensedbylipopeptidealsoindicatedthatexpressionoftheproteinwasclearlyobservedatchargeratioof6.0-7.0.Insummary,thefullysynthesizedlipopeptidecomposedofhighlypositivechargeaminoacidsof lysineandargininehasability to condenseDNAefficiently, forma stablenanoparticle,non-toxictothetargetcellsandhascharacteristicsasapotentialtransfectionagent.Keywords:lipopeptide,particlesize,celltoxicityandtransfection.


102


GELATINSASNATURALCOMPOUNDANDITSPROPORTION-RATIOWITHGLIPTINSTODIPEPTIDYLAMINOPEPTIDASEIV(DP-4)INHIBITION

YoniAtma1*,HanifahNuryaniLioe2,EndangPrangdimurti2,

HermawanSeftiono1,Moh.Taufik1,DitaFitriani1,AponZaenalMustopa3

1*DepartmentofFoodScienceandTechnology,TrilogiUniversity,FacultyofBioindustryKalibata,Jakarta,12760,Indonesia

2DepartmentofFoodScienceandTechnology,FacultyofAgriculturalTechnology,BogorAgriculturalUniversity,Dramaga,Bogor,16680,Indonesia

3BiotechnologyResearchCentre,IndonesianInstituteofScience,Cibinong,Bogor,16912,Indonesia


ABSTRACT

It has been known that inhibition of dipeptidyl aminopeptidase (DP-4) become importanttherapeuticapproachfortype2diabetesmellitus(T2DM)throughincretinregulationcausingtheseekingofDP-4inhibitordynamicallygrowing.Theaimsofthisresearchweretodeterminetheproportion-ratioinhibitoryactivityofgelatinestowardDP-4bycomparingittosyntheticgliptin.Therewerethreetypesofgelatinethathavebeentestedfortheir inhibitoryactivityincludingbovinegelatine,fishskingelatineandfishbonegelatine.Successively,eachgelatinehasproteinconcentrationof2.19±0.008mg/ml,1.70±0.002mg/mland1.27±0.004mg/ml.TheinhibitoryactivityofgliptinhasbeenpatentedandcommercializedasDP-4inhibitorwastestedat various concentration (0.0001-0.01ng/ml). It showed a higher inhibitory activity by theincreaseofitsconcentration.Theinhibitoryactivityofbovine,fishskinandfishbonegelatinewith 0.01 ng/ml of gliptin as standard ratio was 15.6±2.7%, 9.8±0.3%, and 18.2±1.9%,respectively.Theinhibitoryactivityofbovine,fishskinandfishbonegelatinewere16.9±0.8%,10.8±1.6%,and19.8±0.3%respectivelyin0.001ng/mlofgliptinasstandardratio.When0.0001ng/ml of gliptin was used as standard ratio, the inhibitory activity of bovine gelatine was18.8±2.3%,fishskingelatinewas11.8±0.9%,andfishbonegelatinewas21.8±1.4%.TheTukey’shonestsignificanttest(0.05)foundtheinhibitoryactivityagainstDP4betweenbovinegelatineand fish bone gelatinewas non-significant, but it has a significant differencewith fish skingelatine.Keywords:dipeptidylpeptidaseIV(DPP-IV),incretin,inhibitoryactivity,sitagliptin


103


PHENOLICCOMPOUNDSCONTENTANDANTIOXIDANTACTIVITYOFELEPHANTGINGER(ZINGIBEROFFICINALEROSCOE),REDGINGER(ZINGIBEROFFICINALEVARRUBRUM),

ANDEMPRITGINGER(ZINGIBEROFFICINALEVARAMARUM)

NurulMahmudati1*,PoncojariWahyono1,DjoniDjunaedi2

1BiologyEducationDepartment,FacultyofEducation,UniversityofMuhammadiyahMalang

2DepartmentofMedicine,FacultyofMedicine,UniversityofMuhammadiyahMalang*E-mail:[email protected]

ABSTRACT

Secondarymetaboliteespeciallyphenoliccompoundsbecomeanimportantconcernforrecentyears,becauseittakesaroletodecreasetheriskofsomedegenerativediseases.Gingerisrichwithphenolic compounds.However, there is still less informationabout the comparisonofphenoliccompoundsandantioxidantactivityinthethreevarietiesofgingerinIndonesia.Thisis a descriptive quantitative research and aimed at determination of the total phenoliccompoundsandantioxidantactivityintherhizomeofelephantginger,redginger,andempritginger.Thedriedgingerswereextractedbytwotypesofextractionprocesses,i.e.infusionanddecoctionbeforeanalysis.ThephenoliccompoundanalysedusingtheFolin-Ciocalteumethod,while antioxidant activitywas done by using DPPHmethod andmeasured by usingUV-VISSpectrophotometer.BasedonANOVAtestresult,thehighestphenolicwasredginger12.2533mgGAE/g(infusion)and22.9767mgGAE/g(decoction)followedbyemprit9.5033mgGAE/gand17.6500mgGAE/g,elephantginger7.6400mgGAE/gand15.4530mgGAE/g.However,thehighestantioxidantactivitybyinfusionprocesswasfoundinredgingerof79.83%followedby 70.43% and 61.70% in emprit ginger and elephant ginger, respectively. Conversely, thehighestantioxidantactivitybydecoctionwasfound78.76%inempritginger,followedby70.56%and60.93%forredgingerandelephantginger,respectively.Keyword:antioxidantactivity,phenoliccompounds,infusion,decoction.


104


ANTIOXIDANTANDANTIAGINGOFNepheliumlappaceumPEELSEXTRACTANDITSCOMPOUNDS

WahyuWidowati1,*,HeddyHerdiman1,AgniaNursyita1,RizalRizal2,AnnisaAmalia2,Hanna

SariW.Kusuma2,RismawatiLailaQodariah2,FajarSukmaPerdana2,ZakiyatulKhoiriyah2,AnnisaArlisyah2,SatrioHaryoBenowo2,NiLuhWismaEkayanti2

1)MedicalResearchCenter,FacultyofMedicine,MaranathaChristianUniversity,JlProfDrg

SuryaSumantriNo65Bandung40164,WestJava,Indonesia2)ArethaMedikaUtama,BiomolecularandBiomedicalResearchCenter,Jl.BabakanJeruk2,

No.9,Bandung40163,WestJava,Indonesia*E-mail:[email protected]

ABSTRACT

Agingisanaturalprocessinhumansasaresultofoxygen-derivedfreeradicalaccumulationwhich leads to the activation of hyaluronidase, collagenase, and elastase, that can furthercontributetocellularandtissuedamage.Antioxidantscancounteractfreeradicalswhereitcaninhibittheagingprocess.ThisstudyaimedtodetermineantioxidantandantiagingpropertiesofNepheliumlappaceumpeelsextracts(NPE)anditscompounds,gallicacid,caffeicacidandcorilagin.ThephytochemicalanalysisofNPEwasperformedwithFarnsworthmodifiedmethod.Antioxidantactivitieswereperformedbymeasurementof2,2-diphenyl1-pichylhydazyl(DPPH)scavenging activity, Ferric Reducing Antioxidant Power (FRAP), 2,2’-azino-bis (3-ethylbenzothiazoline-6-sulphonic acid) (ABTS) reducing activity assay, while antiaging assaywas observed through inhibitory of elastase, collagenase, and hyaluronidase activities.Phytochemical analysis showed the presence of tannin and terpenoid in high level. Inantioxidantactivity,NPEshowedlowestDPPHandABTSactivity(IC50=8.06µg/mL;2.35µg/mL,respectively)while in FRAP reducingactivity,NPEhasmoderateactivity (IC50 =2.35µg/mL)comparedtogallicacid,caffeicacid,andcorilagin.Inantiagingactivity,NPEshowedthelowestcollagenaseinhibitoryactivity(IC50=300.77μg/mL),butexhibitedhighestactivityinelastaseandhyaluronidaseinhibitoryactivity(IC50=6.94μg/mL;0.72μg/mL,respectively).Insummary,NPEpossessthelowestantioxidantandantiagingcomparedtocorilagin,caffeicacidandgallicacid.Keywords:Nepheliumlappaceum,Antioxidant,Antiaging,GallicAcid,CaffeicAcid,Corilagin.

.


105


IRONENCAPSULATIONUSINGGLUCOMANNANMODIFIEDWITHACETICACIDASAMATRIX

DyahH.Wardhani*,NurRochati,AuliaChusnulita,DwiPurwati

ChemicalEngineeringDepartment,DiponegoroUniversity

Jl.Prof.Sudarto,Tembalang-Semarang50275*E-mail:[email protected]

ABSTRACT

Irondeficiencyisaseriousproblemthataffectshumanresourcesquality.Onestrategytomeetthe ironrequirements isbyaddingthe ironcompoundsdirectly to the foods.However, thisstrategycomeswiththereceptionoftasteandaromaproblems.Moreover,thisdirectadditionallowsirontocontactwithinhibitorcompoundsinthefoodssuchasphyticacidandtanninswhichcauseadecreaseiniron.Theseproblemscouldbeovercomebyencapsulationmethod.Encapsulation limits iron contactwith other components hence protect from the oxidationwhichleadstoundesirablesensorialchanges.Oneofthepotential localmaterialsasanironencapsulation material is glucomannan. This hemicellulose has an excellent film-formingcapability which stable in both cold and hot water, water soluble, biodegradable andbiocompatible. However, the hydrogen bonding could be introduced during glucomannanpurification and drying process and reduce its solubility. Introducing acetyl group helps inincreasingitssolubilityandhelpsinthedrugreleasesystem.Theobjectiveofthisworkswastostudytheimpactofacetylationonglucomannancharacteristicsanditsperformanceasanironencapsulant. The results showed that a higher concentration of glucomannan reduces thephysicochemical properties of acetylated glucomannan and its encapsulation efficiency.Reverse results were observed in an increasing acetic acid concentration. The highestencapsulation efficiency (53.8%) was achieved in themost concentrated acetic acid of 5%glucomannan.Allmodificationsshowasimilarpatterninironreleaseinwhichaninitialburstwas observed in the first 30 minutes period of release in both pH 1.2 and 6.8 solutions.AcetylationdidnotaffectsignificantlyonthereleaserateofironinthebothpH.Keywords:acetylation,encapsulation,glucomannan,iron


106


ANTIDIABETESEFFECTOFELECTROLYZEDREDUCEDWATERINTYPE2DIABETESMELLITUSPATIENTS

PoppyDiahPalupi1*,MetrikanaNovembrina1,FitaRahmawati2,TriMurtiAndayani2

1DepartmentofPharmacologyandClinicalPharmacy,AkademiFarmasiNusaputera,Jalan

MedohoIIINo.2Semarang50162,CentralJavaProvince,Indonesia2DepartmentofPharmacologyandClinicalPharmacy,FacultyofPharmacy,GadjahMada

University,SekipUtaraRoad,Yogyakarta55281,Indonesia*E-mail:[email protected]

ABSTRACT

Diabetesmellitus (DM) isametabolicsyndromewithacharacteristic featureof increasingbloodglucose concentration, impaired metabolism of carbohydrates, proteins, and fats, which isassociated with deficiency of insulin secretion. Electrolyzed Reduced Water (ERW) iselectrochemicallyreducedwater,containsmanyatoms,hydrogenmolecules,mineralnanoparticlesandhas apreventiveeffectondiseases causedbyoxidative stress suchasdiabetes.Aimof thepresentstudywastoinvestigatetheeffectofeightweeksadministrationofERWintype2diabetesmellitus patients. The study was performed a randomized, 2 x 4 week period, double-blind,crossover design in 24 T2DM patients controlled by diet and exercise therapy. The patientsconsumed2000mL/dofERWfor8weeks.IntakeofERWfor8weeksreducedrandombloodglucoselevel5.5%and5.7%forpostprandialbloodglucose(p>.05).Inconclusion,administrationofERWfor8weekshasnoassociatedwithloweringbloodglucoselevelsinT2DMpatients.Keywords:Electrolyzedreducedwater,type2diabetesmellitus,bloodglucoselevels,patients


107


INFLAMMATIONSTATUSPROFILEINYOUNGADULTMENOBESE&NONOBESE

STUDYOFHIGHSENSITIVITYC-REACTIVEPROTEIN(hsCRP)SERUMLEVEL

SulaemanA*,ZilIkramF,Patonah

DepartmentofPharmacology,BandungSchoolOfPharmacySoekarnoHattaStreetNo754BandungCityWestJava40614


ABSTRACTObesity is often accompanied by low grade chronic inflammatory conditions especially inwhiteadipose tissue (WAT). The current good inflammatory markers was high sensitivity c-reactiveprotein (hsCRP) as a predictor for the risk of cardiovascular disease. The aims of this studydeterminedthelevelofCRPinyoungadultmen.Thisstudywasanobservationalstudywithcross-sectional study design involving 74 male subjects consisting of obese subjects who had waistcircumference≥90cmandnonobesewhichhadwaistcircumference<90cmwithagerange18-25years.Datacollectionusingquestionnaires,anthropometricmeasurementsandcollectionofbloodspecimensforbiochemicalexamination.ThedataobtainedwereanalyzedstatisticallyusingSPSSver20.Theresultsofthestudywere37obesesubjects,hadhighCRPlevelsof22people(59.46%)and37nonobesesubjectshadhighCRP levelsof11people(29.73%).Obeseyoungmenhave5timesgreaterriskofcardiovasculardiseasethannonobeseyoungmen.TheconclusionofthestudyshowedthatobeseyoungadultmenmightbehavehigherlevelsofCRPcomparedwithnonobeseyoungadultmen.Keyword:Inflammationstatus,hsCRP,Obesity


108


UV-VISSPECTROSCOPICMETHODFORSIMULTANEOUSESTIMATIONOFCURCUMINANDPIPERINEINDISSOLUTIONSAMPLESCONTAININGCURCUMALONGAANDPIPERNIGRUMSOLID

DISPERSION

YosiBayuMurti1,*,YustinaSriHartini2,H.W.Frijlink3,WouterL.JHinrichs3,DewiSetyaningsih2

1FacultyofPharmacy,UniversitasGadjahMada2FacultyofPharmacy,SanataDharmaUniversity

3)DepartmentofPharmaceuticalTechnologyandBiopharmacy,UniversityofGroningen,TheNetherlands

*E-mail:[email protected] use of piperine to curcumin in oral administration has been reported to improvecurcuminbioavailabilitydueto its inhibition incurcuminglucuronidation1.However,duetotheirlipophilicity, these compounds exhibit poor dissolution. Solid dispersion is a promising way toenhancedissolutionoflipophiliccompounds2.Dissolutionisbeingthekeyparameterofasuccessfulformulation.Duetoaneedofquickanalysisofhugesamples,spectrophotometrycanbearelevantmethod during evaluation of the formulas. The aim of this studywas to validate spectroscopicmethod for simultaneous determination of curcumin and piperin concentrations in dissolutionsamplescontainingsoliddispersionformulationofCurcumalongaL.rhizomeandPipernigrumL.fruit extracts. Stock solutions of curcumin and piperine were prepared in methanol at aconcentrationof1ppm.The seriesof themixtureof curcumin-piperine for calibration solutionswere prepared by diluting the stock solutions with dissolutionmedium as thematrix (0.5%-wtSodiumLaurylSulfatein20mMsodiumphosphatebufferofpH6.0).Thevalidationmethodwascarried as per ICH guidelines. The mixture of curcumin and piperine in dissolution medium ofconcentrationof2and1ppmwassubjectedtooverlayscan inaUVspectrophotometer ineachλmaxof430and335.5nmforcurcuminandpiperine,respectively.Theresultsdemosntratedthatthe calibration curves of curcumin and piperine show linearity with r = 0.9989 and 0.9990 atconcentrationrangesof0.2to5ppmforcurcuminandpiperine,respectively.ThemethodprovidesprecisionandaccuracyasconfirmedbyAOAC3Moreover,themethodshowsitshighsensitivitywithLOD and LOQ of 0.2 and 0.7 ppm for curcumin and piperine, respectively. For conclusion, thedeveloped method is applicable to simultaneous determination of curcumin and piperineconcentrationsinthedissolutionsamples.Keywords:curcumin,piperine,CurcumalongaL,PipernigrumL,dissolution,UV-Visspectroscopy


109


PREPARATIONANDEVALUATIONOFTHEEFFERVESCENTGRANULESFROMBREADFRUIT

(Artocarpusaltilis(Parkinson.)Fosberg)LEAFETHANOLEXTRACT

VinaF.Otuh*,A.Hasrawati,WahyuniWahid,MuzdalifahRamly

Farmasi,FakultasFarmasi,UniversitasMuslimIndonesia,90231*Email:[email protected]

ABSTRACT

Thebreadfruitleaf(Artocarpusaltilis(Park.)Fosbergcontainssomenutritioussubstancessuchashydrocyanic acid, acetylcholine, potassium, tannin, flavonoids, and riboflavin advantageous toreduceglucoseinbloodcells.ThemainpurposeofthisformulationisnoneotherthantodevelopnaturalresourcesinIndonesiabyutilizingbreadfruitplantsbyutilizingbreadfruitplantsformulatedinto effervescent granules (EG). The GE preparations are made with variations of lactose asadsorbent, tartaric and citrate acid as a source of acid, and sodiumbicarbonate as a carbonatesource.Themethodusedwaswetgranulation.TheGEwasevaluatedthephysicalcharacteristicswithparametersofwatercontent,porosity,flowtime,angelofrepose,Carr'sIndexanddispersedtime.Thewatercontentsoftheformula(F1,F2,F3)were3.48%,3.90%,and2.38%respectively.TheporosityofthepreparationshowsF1isattherequiredvalueof40.07%.Allformulasshowaflowtimeoflessthan10g/secandtheangleofreposeshowstheflowpropertiesareverygood.OnlyF1andF3showCarr’s Indexbelow20%.Theresultsof thedispersedspeedtestwere3.07minutes,2.21minutesand1.27minutes.Theconclutionofthisresearch is effervescentgranuleformulation from breadfruit leaf extract (Artocarpus altilis (Parkinson.) Fosberg) with the mostoptimumphysicalcharacteristicsisF1.Keyword:breadfruitleaf,granules,effervescent,ethanolicextract,antidiabetic.


110

PosterPresentation [PP-01]

ANTIMICROBIALACTIVITYOFSOMEINDONESIANPLANTSPECIESFROMTHEGENUSFICUS

DikiPrayugoWibowo1,2,*,NengFisheriKurniati1,KomarRuslanWirasutisna1,MuhamadInsanu1*

1DepartmentofPharmaceuticalBiology,SchoolofPharmacy,InstitutTeknologiBandung,Jl

Ganesha10,Bandung40132,WestJava,Indonesia.2IndonesianSchoolofPharmacy.Jl.SoekarnoHatta354,Bandung40266WestJava,Indonesia


ABSTRACT

Ficus(Moraceae)isoneofthebiggestgeneraofangiospermwithinexcessof800speciesand2000assortments in the tropic and sub-tropic around the world. Plant species of the genus Ficus(Moraceae)areknowntoproduceavarietyofsecondarymetabolites,whichhavevariousbiologicalactivities,manyplantsofgenusFicusareusedinthetreatmentofinfectiousdisease.Thepurposeofthisresearchwastoexaminetheantimicrobialactivityof10IndonesianFicusspeciesthathadnotbeen subjected to previous pharmacological testing. Organic extracts of plants from the genusFicuswerefilteredtotestantimicrobialactivityagainstbacteria.TheantimicrobialactivityofextractswasassessedagainstStaphylococcusaureus,Bacillussubtilis,Pseudomonasaeruginosa,Escherichiacoli,Candida albicans, andAspergillus nigerby utilizing agar paper disc agar diffusion andmicrobrothdilutionmethods.Theresultsofthepreliminarystudyusingthepaperdiskdiffusionmethodshowedthat10methanolleavesextractsofFicusspecieshadantibacterialactivityagainstatleast2of4testbacteria(atconcentrationsof0.8g/mL,0.4g/mLand0.2g/mL)andnoneoftheextractsshowedactivityagainstfungioryeast.ExtractsofFicusspeciesshowedselectivityagainstbacteriawithMICandMBCintherangeof250-1000µg/mL.Plantextractsarearichsourceofantibacterialpotentialtocombatbacterialdiseases.OurresultsshowedthatextractsfromFicusconsociate,Ficusglabella,and Ficus ribeshave promising antibacterial activity, with potential applications in thetreatmentofbacterialdiseases.Keywords:Ficus,antibacterial,antimicrobial


111

PosterPresentation [PP-02]DEVELOPMENT,CHARACTERIZATIONANDPERFORMANCEEVALUATIONOFTRANSFEROSOMEGELOFIBUPROFENASATRANSDERMALDRUGDELIVERYSYSTEMUSINGNANOVESICULAR

CARRIER

1FitriantiDarusman*,2RifnieRaisya,3SaniEgaPriani1,2,3ProgramStudiFarmasi,FakultasMatematikadanIlmuPengetahuanAlam,UniversitasIslam

Bandung,Jl.TamansariNo.1Bandung,WestJava,Indonesia40116


ABSTRACTIbuprofen (2- (4-isobutylphenyl) propionate) is a class of non-steroidal anti-inflammatory drugs(NSAIDs)andisclassifiedinclassIIofBiopharmaceuticClassificationSystem.Ingeneral,NSAIDsthatareusedorallycausesideeffectsofgastriculcers.Forthisreason,ibuprofenwasformulatedintotransfersome to enhanced transdermal drug delivery using nanovesicular carriers1,2,3,, whileavoidingoralsideeffects.Thepurposeofthisstudywastoformulatetransfersomibuprofeningelpreparations,thencompareitsperformancetogelcontainingpureibuprofen.Transfersomewereformulatedintofourformulaswithconcentrationratioofphospholipon90G:span80forFI,FII,FIII,danFIVwere90:10;85:15;80:20;and75:25usingdirectmixingmethod,theinvitroskinpermeationstudieswasdeterminedusingFranzdiffusioncelltothetwoformulaofviscolamgelthatcontaining1%ofibuprofenwhichareibuprofentransfersomegelandpureibuprofengel.TheresultshowedthatFIVwasthebesttransfersomewithentrapmentefficiencywas92,95%andhadparticlesizeof1,286 µm. Ibuprofen transfersome gel had been shown to increase in vitro skin permeation ofibuprofen, cumulative penetrationwas 1286,64 µg.cm-2, fluxwas 214,44 µg.cm-2.hours-1and theparticlesizewas0,986µm,whilecumulativepenetration, fluxandparticlesizeofgelcontainingpureibuprofenwere620.92µg.cm-2,103.49µg.cm-2.hours-1and0.496µm,respectively.Keywords:Transfersome,ibuprofen,transdermaldrugdelivery,Franzdifussioncell.


112


THEPEEL-OFFGELMASKOFGREENTEALEAFEXTRACT

(CamelliasinensisL.):FORMULATIONANDANTIOXIDANTACTIVITY

SetyoNurwaini*,DhiahAyuPermatasari

FacultyofPharmacy,UniversitasMuhammadiyahSurakartaJl.AhmadYaniTromolPos1Pabelan,Surakarta,Indonesia57169


ABSTRACT

Green tea leaf extract is a potential antioxidant with IC50of 3.17 μg/ml (1). The use of topicalproducts that containing antioxidant are highly recommended for preventing skin from photo-inducedaging(2).[1]Thisstudyaimstoformulatethepeel-offgelmaskofgreentealeafextractandmeasure itsantioxidantactivity.Theextractwasobtainedbymacerationwith96%ethanol.Thepeel-offgelmaskswereformulatedusingvariousconcentration[2]of2-3.5%w/vHPMCasagellingagent. Thephysicalpropertiesof formulaswere tested includedorganoleptic,homogeneity,pH,viscosity,rheology,spreadabilityanddryingtime.AntioxidantactivityofformulawasdeterminedusingDPPH.Theresultsshowedallformulashadtherequirementsofgoodpeel-offgelmask.TheIC50of the peel-off gel mask of green tea leaf extract was 15.83 μg/ml. Conclusion:The resultindicatesthatthepeel-offgelmaskofgreentealeafextracthasagoodpotentialtobedevelopedasacosmeticproduct.Keywords:peel-offgelmask,CamelliasinensisL.,antioxidant


113


ACUTETOXICITYTESTOFETHANOLEXTRACTSOFCAWATHANOMAN

(UrariacrinitaL.)ROOTUSINGOECD425GUIDLINE

HelminaWati*1,RahmiMuthia1,PinusJumaryatno2,FaridaHayati2

*1SekolahTinggiIlmuKesehatanBorneoLestariBanjarbaru,SouthBorneo,Indonesia.2DepartementofPharmacy,FacultyofMathematicandNaturalSciences,UniversitasIslam

Indonesia*E-mail:[email protected]

ABSTRACT

CawatHanoman(UrariacrinitaL.)rootisaplantthathasbeenusedempiricallybythepeopleofSouthKalimantanasanaphrodisiac.InthestudyofWatietal.(2017)ethanolextractoftherootofCawatHanoman(U.crinitaL.)hasanactivityasanaphrodisiac.ThepurposeofthisstudywastodeterminetheLD50value,toknowthetoxicsymptomsthatariseaftertheethanolextractoftherootofCawatHanoman(U.crinitaL.)inmaleWistarratsandtoknowtheclassificationofacutetoxicity.AcutetoxicitytestingiscarriedoutusingthemethodadoptedfromOECDguidelineno425:AcuteoralToxicity(UpandDownProcedure).Theresultsofthelimittestshowedthattheethanolextractethanolof cawathanoman LD50is greater than2000mg /kgBW.Therewereno clinical signsoftoxicityontheeye,respirationsystem,behavior,theautonomicandsomatomotorsystematadoseof2000mg/kgBW.Atthedosenotcausedeathintherat,anddoesnotcausesignificantweightloss.Conclusion:AccordingtotheLoomisclassification,theethanolextractofcawathanomanhasalowtoxicity.Keywords:CawatHanoman,AcuteToxicity,LD


114

PosterPresentation[PP-06]

IMMUNOMODULATORYEFFECTOFETHYLACETATELEAFEXTRACTOFPERMOT(PASSIFLORAFOETIDAL.)AGAINSTTHESECRETIONOFANTIBODYANDDELAYEDTYPEHIPERSENSITVITYIN

VIVO

AndiEmelda1*,AuliaWati2,ArrafiqAlAmmarie3,andRadenRizalSaputra4

1,2,3,4FacultyofPharmacy,UniversitasMuslimIndonesia,Jl.UripSumohardjoKM.5,Makassar90231,Indonesia


ABSTRACT

Immunomodulatorsarecompoundsthatcanimprovethebody'sdefensemechanismssotheycanrestoreandrepairthefunctionoftheimpairedimmunesystem.TheresearchaimstodeterminetheeffectofimmunomodulatoryextractofethylacetateofPassiflorafoetidaL.leaf(EEPL)onratantibody secretion after induced sheep red blood cell (SRBC) and delayed type hypersensitivity(DTH). Examination of antibody secretion was performed using 25 rats divided into 5 groups:control,negativecontrol(SRBC),EEPLgroupsdoseof200,400,and600mg/kgbw.Alltestanimals,exceptcontrolgroup,inducedSRBConthe3rddayintraperitoneally.Theextractisgivenfor7days.TheDTHtestwasdividedinto5groups:control,negativecontrol(SRBC),EEPLgroupsdoseof400,800, and 1200mg/kgbw. The groups, except control group, were given antigen on the 3rd dayintraperitoneallyandonthe7thdaywereadministeredintraplantarly.ThethicknessofthesolesofthefeetwasmeasuredusingaplethysmometerbeforeandafterinjectionofantigenatT4,T24andT48.ThedatawereprocessedstatisticallybyKruskal-WallisandadvancedtestbyMann-Whitney.ThegroupofcontrolnegativeagainstthegroupsofEEDPwassignificantlydifferent(P<0.05).Theantibodytiterofthegroupsgiventheextractwerehigherthanthatoftheantigen-inducedgroupwithouttheadministrationoftheextract.TheresultsshowthatthegroupsofEEDPcanincreaseantibody secretion. The test of DTH showed that the group of negative control to EEPL groupsshowedsignificantlydifferent(p<0,05).Theresultsshowthattherearedecreasesofsolesofthefeet volume of mice at T48. for control (0.15±0.05), SDMD (0.19±0.005), dose of 0.4 g/kgbb(0.11±0.005),0.8g/kgbw(0.14±0.02)and1.2g/kgbw(0.14±0.02).EEPLof400mg/kgbwand600mg/kgbwcanincreasethesecretionofantibody.Thedoseof0.4g/kgbwshowedadecreaseofmicefootvolumebetterthanotherdoses.Keywords:Immunomodulatory,PassiflorafoetidaL,extract


115


DISSOLUTIONENHANCEMENTOFATHORVASTATINCALCIUMBYSELFNANOEMULSIFYINGDRUG

DELIVERYSYTEMUSINGCREMOPHORANDTRASCUTOLASSURFACTANTS

SaniEgaPriani*,Annisa,G.C.EkaDharmaPharmacyDepartment,BandungIslamicUniversity(UNISBA)

TamanSari1Bandung40116*E-mail:[email protected]

ABSTRACTAthorvastatin calcium is HMG-CoA reductase inhibitor, one of the most popular medicines fortreatmentofhighcholesterol[1,2].AthorvastatinisBCSclassIIdrugwithlowaqueoussolubilityandthereforelowbioavaibility(14%)[3,4].SelfNanoemulsifyingDrugDeliverySystem(SNEDDS)knowncanimprovedissolutionrateandoralbioavaibilityofdrugs[5,6,7].ThisstudyaimstoincreasethedissolutionrateofAthorvastatincalciumbyformulatingintoSNEDDS.Theoptimizationformulawascarriedoutusingvariouscomparisonsofoilandmixedsurfactants(1:9;1:8;1:7;1:6)danvariouscomparisonofsurfactantsandcosurfactant(3:1;2:1;1:1).TheformulatedSNEDDSwereevaluatedfortransmittancepercentage,dispersibility,robustness,thermodynamicstability,globulsize,anddissolutiontests.ThebestformulaofSNEDDScontainatorvastatincalcium10mg/mLwitholeicacidastheoilphase,cremophorRH40asthesurfactant,andtranscutolasthecosurfactant,withratiobetweenoilwithmixtureofsurfactantswas(1:7)andratiobetweensurfactantandcosurfactant(3: 1). Thepreparationwasmeets the requirementsof the transmittancepercentage (93.56%±0.115), dispersibility time (34.67 seconds ± 0.644), stable on robustness and thermodynamicstabilitytestsandhasaverageglobulsize87nm.TheresultshowedthatSNEDDSofatorvastatincalciumcouldimprovethedissolutionofathorvastatincalciumcomparedwithpuredrug.94,6%ofdrugwasdissolutedin45minutes.SNEDDSpreparationcontainingathorvastatincalciumwasmeetphysical and stability requirements. SNEDDS formulation could enhance the dissolution rate ofAthorvastatincalcium.Keywords:Athorvastatincalcium,SNEDDS,Dissolution,CremophorRH40,Transcutol


116


EffectofBangleRhizomeExtract(ZingibercassumunarRoxb.)AgainstGastricUlcerinAspirin-

inducedRats

AriYuniarto1,*,ToniAbdulRahman1,ElisSusilawati1,DadangJuanda2

1PharmacologyResearchGroup,BandungSchoolofPharmacy,Jl.Soekarno-HattaNo.754,Bandung,WestJava,Indonesia.

2PharmaceuticalBiologyResearchGroup,BandungSchoolofPharmacy,Jl.Soekarno-HattaNo.754,Bandung,WestJava,Indonesia.


ABSTRACT

Gastriculcerisoneofthecommonseriousdiseasesingastrointestinaltractandgivecontributionagainst morbidity and mortality. Pathophysiology of gastric ulcer is an imbalance betweenaggressiveandmucosaldefensivefactors.Increaseofaggressiveanddecreaseofmucosaldefensivefactorsassociatedagainstdevelopedofgastriculcerdisease.Theobjectiveoftheresearchwastoevaluate gastric ulcer healing effect of bangle rhizome extract (Zingiber cassumunar Roxb.) inaspirin-induced rats model.Antiulcer activity of bangle rhizome extract was evaluated throughseveralparametersinvolvesgastricacidity,numberofulcers,diametersofulcers,ulcerindex,andhealingratio.Theresultswereexpressedasmean±SD.Statisticalanalysisofcomparisonbetweengroups was conducted by ANOVA and were coupled with Dunnet’s test. Furthermore,histopathologicalofthestomachwasperformedusinghematoxylin-eosinstained.Theresultsshowedthatthegroupswhichgivenbanglerhizomeextractaresignificantdifferentforgastricacidity,numberofulcers,diametersofulcers,ulcerindex,andhealingratiocomparedtothecontrolgroup.Histopathologicalstudyofthestomachulcersshowedtissuesregenerationingroupweretreatedbybanglerhizomeextractcomparedtothecontrolgroup.Keywords:ZingibercassumunarRoxb.,Extract,Ulcer,Aspirin.


117


TOPOISOMERASEINHIBITORCOMPOUNDISOLATEDFROMN-HEXANEFRACTIONOFCEMPAKA

KUNINGSTEMBARK(MicheliachampacaL.)

AdeZuhrotun*,FrederickAlexander

FacultyofPharmacyUniversitasPadjadjaranJl.RayaBandungSumedangKM.21,Hegarmanah,Jatinangor,KabupatenSumedang,JawaBarat

45363*E-mail:[email protected]

ABSTRACTAlongwith the increasing incidenceandnumberofdeathsdue tocancer,effectiveandefficientresearchofanticanceragentsisneeded,asoneofthemcanbederivedfromplants.Then-hexaneandethylacetatefractionofCempakaKuningstembark(MicheliachampacaL.)wereshowntohaveactivity as a topoisomerase inhibitor. This study aimed to isolate and identify groups of activecompoundscontainedinthen-hexanefraction.Thedryextractofmethanolisfractionatedbyliquid-liquid extractionmethodwith three different solvents. The n-hexane fractionwas separated byvacuum liquid chromatography using the gradient mixture of n-hexane: ethyl acetate elutes.Furthermore,theseparationwascompletedbyusingcolumnchromatographyagainstsubfractionVIandprovedtobeactivewithamechanismbasedyeastbioassaymethod.ThepurificationofthecompoundwascarriedoutbypreparativeTLCandthe identificationofthegroupofcompoundswithvariousspecificsprayreagents.Bythoseprocess,isolateMCNH-Iwereobtainedandidentifiedasagroupof flavonoid.Theresults testusingmechanism-basedyeastbioassayshowedthattheisolateactiveasatopoisomeraseIinhibitorwithIC1283,004μg/mL..Keywords: Michelia champaca L., cempaka kuning bark, mechanism-based yeast bioassay,topoisomeraseIinhibitorI.


118

PosterPresentation [PP-11]THEEFFECTIVENESSOFIN-SITUGELFORCHLORAMPHENICOLOPTHALMICPREPARATIONWITH

BASEPOLOXAMER407ANDHPMCAGAINSTSTAPHYLOCOCCUSAUREUSATCC29213ANDPSEUDOMONASAERUGINOSAATCC27853

InsanSunanKurniawansyah1,2,*,NoriscaAlizaPutriana1,TanMeiLee3

1DepartmentofPharmaceuticsandPharmaceuticalTechnology,FacultyofPharmacy,UniversitasPadjadjaran,Jatinangor,Indonesia

2PUSDIDrugDeliveryandDrugDisposition,FacultyofPharmacy,UniversitasPadjadjaran,Jatinangor,Indonesia

3FacultyofPharmacy,UniversitasPadjadjaran,Jatinangor,Indonesia*E-mail:[email protected]

ABSTRACT

In-situgelisasimpleliquidtransparentpolymersolutionunderstorageconditions,butturnsintoaviscoelasticgelafterenteringtheeyeduetothephasetransitionpropertiesofthepolymerthatincreasetheresidencetimeinocularorganandbioavailability,enablingthedeliveryofreproducibledoses and improving patient compliance. The aim of the present study was to formulate andevaluatetheantibacterialeffectivityofchloramphenicolin-situophthalmicgelwithbasepoloxamer407andHPMCbaseagainstStaphylococcusaureusandPseudomonasaeruginosa.Theoptimizationofophthalmicgelpreparationbythefactorialdesignmethodhasbeencarriedoutinordertoknowthebestformulaofalltheformulasemployedwith0.5%chloramphenicolactivesubstance,whereineach formula was obtained from high concentration and low concentration of each base. Themeasurement of the antibacterial effectivity against Staphylococcus aureusATCC 29213 andPseudomonasaeruginosaATCC27853byone-wayANOVAanalysisshowedthatformulawithbasepoloxamer4075%(F1)gavethebestresult.F1hasadiluteconsistency,clearandstableduring28days storage time when effectiveness test performed. Chloramphenicolin-situgel with basepoloxamer 407 and HPMC were effective against Staphylococcus aureusATCC 29213 withintermediate to sensitive category, and Pseudomonas aeruginosaATCC 27853 with sensitivecategoryinaccordancetotherequirementsoftheClinicalandLaboratoryStandardsInstitute(CLSI).Keywords: chloramphenicol, HPMC, in-situ gel, poloxamer 407, Pseudomonas aeruginosa,Staphylococcusaureus,


119


CONSTRUCTIONANDEXPRESSIONOFSYNTHETICGENEENCODINGMPT64ASEXTRACELLULARPROTEININESCHERICHIACOLIBL21(DE3)EXPRESSIONSYSTEM

SriAgungFitriKusuma1,*,IdaParwati2,TinaRostinawati1,MuhammadYusuf3,4,Muhammad

Fadhlillah4,6,LailyD.Tanti3,RisaR.Ahyudanari3,YayaRukayadi5,TotoSubroto3,4

1DepartmentofBiologyPharmacy,FacultyofPharmacy,PadjadjaranUniversity,Sumedang,WestJava,Indonesia45363,2ClinicalPathologyDepartment,FacultyofMedical,PadjadjaranUniversity,Bandung,3DepartmentofChemistry,FacultyofMathematicsandNaturalSciences,Padjadjaran

University,Sumedang,Indonesia,4ResearchCenterofMolecularBiotechnologyandBioinformatics,PadjadjaranUniversity,Bandung,Indonesia,5DepartmentofFoodScience,Faculty

ofFoodScienceandTechnology,UniversitiPutraMalaysia,Serdang,Malaysia,6PT.GenproMultigunaSejahtera,Sumedang,Indonesia


ABSTRACTMPT64proteincandifferentiateamongmycobacteriumgroups,thusitcanbeusedasadiagnosistoolfortuberculosisdetectionusingimmunochromatographymethod.Therefore, largeamountsofMPT64proteinareneededtoproduceantibodiesagainstthesecretedMPT64protein.ThisstudywaspurposedtoproduceMPT64proteinextracellularlybyconstructingpelBsignalpeptide intoexpressionvectorcontainingsyntheticgeneofmpt64fromMycobacteriumtuberculosisH37Rv.Thempt64syntheticgenewasderivedfromgenesequenceofM.tuberculosisH37Rv.Therefore, thempt64genesequenceneedcodonoptimizationusingagraphicalcodonusageanalyzer(GCUA)toimprove gene expression in E. coli. The optimized codon then design and construct into anE.coliexpression vector (pD861-SR) containing such component that high replication, strong RBS,inductionsystemwithrhamnoseandsupplementedwithkanamycinselectionmarkers.ThepelBgene,agenecodingforextracellularproteinswasinsertedintothevector.ThetransformationofpD861-SR plasmid into E. coliBL21 (DE3)was conducted using electroporationmethod and theisolatedplasmidwasverifiedbysequencingmethod.TheoverproductionofMPT64proteinwasinducedby4mMrhamnoseindifferenttimeofinduction.FollowedbycharacterizationofMPT64protein using sodium dodecyl sulfate polyacrilamide electrophoresis (SDS-PAGE). The genesequencingresultsshowedtherewasnonucleotidechangesinthesequencesofmpt64andpelBgenes.BasedonSDSPAGEanalysis,thepelBsignalpeptidecouldtranslocatetheMPT64proteinintothemediumasanextracellularproteinwith amolecularweightof24 kDa.Conclusion:Theexpressionofmpt64genecombinedwithpelBassignalpeptidecouldbeachoicedesigninplasmidrecombinantconstructiontoyieldMPT64asextracellularprotein.

Keywords:MPT64,MycobacteriumtuberculosisH37Rv,pelB,extracellular,Escherichiacoli


120


CHEMICALCOMPOSITIONOFESSENTIALOILOFSTONEREDONION(ALLIUMCEPAL.)FROM

BAYONGBONG,GARUT,WESTJAVA

RiaMariani*,FaridPerdana,TantiJulianti

DepartmentofPharmacy,GarutUniversity,Jati42B,Garut44151,Indonesia


ABSTRACTStoneredonionisonethevarietiesofredonions.Tuberredonioncontainsvolatileoil.ThisresearchstudiedchemicalcompositionofvolatileoilofstoneredonionfromBayongbong,Garut,WestJava.Isolationofvolatileoilwasusingbywaterandvapordistillation.Chemicalcompositionofvolatileoil was analyzed by gas chromatography- mass spectroscopy (GC-MS). Isolation of volatile oilyielded1mlofvolatileoil.GC-MSofvolatileoilidentified32compoundswithmethyleugenolasadominantcompound.Keywords:AlliumcepaL.,stoneredonion,volatileoil


121


UTILIZATIONOFCOCONUTWATERANDDREGSOFSOYSAUCEASSUBSTRATESINCAROTENOID

PIGMENTPRODUCTION

SenoAuliaArdiansyah*,AnggiRestiasari,LandiyaniPutri,EkaNoviana

SekolahTinggiFarmasiIndonesia(STFI),Jl.SoekarnoHattaNo.354(ParakanResik)Bandung40266*E-mail:[email protected]

ABSTRACT

Antioxidants canbe found in natureormade synthetically.Oneof thenature[1] antioxidants iscarotenoids.Neurosporasitophilaalsowasknown[2]asoncommolds thatcontainscarotenoids.Dregsofsoysauceareasoybased[3]wasteandstillhasahighproteincontent.Coconutwatercanbeusedasasubstrateincarotenoidfermentation.NeurosporasitophilaisolatedfromredoncomtoincreaseamountofproductioninPDA(PotatoDextroseAgar)mediaandincubatedfor5daysatroomtemperature.SporesuspensioncreatedfromresultsincubationofNeurosporasitophila.Thesporesuspensionuse[4]dforthenexttestingonSDB(SabouraudDextroseBrooth)media.Makedregsofsoysaucesubstrateandcoconutwaterassubstrateswithconcentrationon10%,15%,20%and25%v/v.ChoosedthegoodconcentrationdregsofsoysauceandcoconutwaterontheSDBmedia.[5]Theresultofcarotenoid ischeckedforabsorbanceusingUV-Visspectrophotometerat450nmandcomparedwithstandardbeta-carotene.Theresultsshowedthatmetalionsadditiononsubstratedregsofsoysauceandcoconutwatercouldincreasecarotenoidsproduction.Dregsofsoysaucedproduce4,829gramscarotenoids;andcoconutwaterproduce3,988gramscarotenoids.Keywords:Neurosporasitophila,coconutwater,dregsofsoy-sauce,substrates,metalions,UV-VisSpectrophotometer


122


THEDIFFERENTEFFECTOFMICRO-SIZEDANDNANO-SIZEDIBUPROFENPARTICLESONTHEDISSOLUTIONRATEOFIBUPROFENTABLETSUSINGDRYGRANULATIONMETHOD

FahjarPrisiska*,IndingGusmayadi,SheilaApridaEkaPutri

FacultyofPharmacyandScience,

UniversityofMuhammadiyahProf.DR.HAMKA,Jakarta*E-mail:[email protected]

ABSTRACT

IbuprofenisacompoundmodelincludedintheBiopharmaceuticalClassificationSystem(BCS)IIthathashighpermeabilityyetlowsolubility.Thisresearchwasconductedtomodifythedissolutionrateby reducing theparticlesize. In this research, thesizeof themicronized ibuprofenparticleswasreducedintonanosizeusing30-hourmillingprocess,resultinginparticleswhichsizeissmallerthan1000 nm. The sizes of micronized and nano ibuprofen were evaluated using the Particle SizeAnalyzer(PSA).Afterthat,granuleswereproducedandevaluated.Thegranuleswerethenpressedinto tablets tobe furtherevaluated.Thedissolution ratewasmeasuredusinga statisticalT-testanalysiswith95%confidencelevel(α=0.05),obtainingsig(0.130)>(α=0.05),indicatingthattherewas nomeaningful between the formulas. The result of this study also showed that nano-sizedibuprofendidnotimprovethedissolutionrateofibuprofentablet.Themicronizedibuprofentabletproduced faster dissolution rate of 4.842%/minute, while the dissolution rate of nano-sizedibuprofentabletwas2.022%/minute.Keywords:particlessized,ibuprofen,dissolutionrate


123


ISOLATION-GUIDEDBYINVIVOANTIHYPERTENSIVEACTIVITYFROMETHYLACETATEFRACTION

OFROSELLE(HibiscussabdariffaL.)

YasmiwarSusilawati1,*,SantiPerawati1,SriAdiSumiwi2,IdaMusfiroh3,RiniHendriani2

1DepartmentofBiologicalPharmacy,FacultyofPharmacy,2DepartmentofPharmacologyandClinicalPharmacy,FacultyofPharmacy,

3DepartmentofAnalysisofPharmacyandMedicinalChemistry,FacultyofPharmacy,UniversitasPadjadjaran,Jl.RayaBandung-SumedangKm.21Jatinagor45363,

WestJava,Indonesia*E-mail:[email protected]

ABSTRACT

Theprevalenceofhypertensioncontinuestoincreaseinmanycountriesintheworld,includinginIndonesia. Many studies had proven that many compounds from plant have antihypertensiveactivity.Previousresearchshowedthatethanolextractofrosellecalyx(H.sabdariffaL.)atdoseof250mg/kgBWanditsethylacetatefractionatdoseof100mg/kgBBcouldsignificantlydecreaseblood pressure (P<0.05). The aim of this study is to determine the antihypertensive activecompound isolated from ethyl acetate fraction of roselle calyx. The separation of ethyl acetatefraction was conducted by using vacuum liquid chromatography method using n-hexane, ethylacetateandmethanolingradientelutionsystem,sothatitwasobtained3sub-fractions(Hs-II-A,Hs-II-B,andHs-II-C).Theexaminationofantihypertensiveactivityofsub-fractionswasperformedby using non-invasive blood pressure method on mice (Mus musculus) and glibenclamide as apositivecontrol.TheresultsshowedthatHs-II-Bsub-fractionatdoseof7.2mg/kgbwperformedthe highest antihypertensive activity by reducing systolic and diastolic as much as 20.08% and19.18%.FurtherseparationofHs-II-BbyusingcolumnchromatographyandpreparativeTLCwasobtained 3 isolates (Hs-II-B1, Hs-II-B2, andHs-II-B3). The examination result ofantihypertensiveactivityofthoseisolatesatthesamedose(2.25mg/kgbw)showedthatHs-II-B1exibitedthehighestantihypertensive activity with the systolic and diastolic inhibition were 17.69% and 17.07%.Meanwhile, the systolic and diastolic inhibition of Hs-II-B2were 4.2% and 3.67%; Hs-II-B3were9.48%and11.61%.Asaconclusion,Hs-II-B1revealedthemostactiveantihypertensivecompoundfromrosellecalyx.

Keywords:Antihypertensiveactivity,roselle,non-invasivebloodpressure,activecompoun


124


ANTIACNEPOTENCYANDGELFORMULATION

FROMPALMSUGAR(ArengapinnataMerr.)ASHEXTRACT

EndahRismawati*,AzizahAnnurFadhilah,EstiRachmawatiSadiyah

ProgramStudiFarmasi,FMIPA-UniversitasIslamBandungJl.RanggamalelaNo.1Bandung40116


ABSTRACTAcne is a common skin diseasewhich caused by the excess production of oil glands that clogsfollicularductsandskinpores,causingbacterialinfections.Acnetreatmentisusuallydonebyusingantibiotics which can inhibit inflammation and kill bacteria, but it has negative effect, such asresistance.Itencouragesresearchertofindanti-acnefromnatural ingredients.ForthepeopleofIndonesia,especiallyPasundancommunity,palm treesareoftenused in traditional ceremonies,householdfurnitureandtraditionalmedicinalmaterials.InGarutdistrictpreciselyinCisewuvillage,palm sugar ash is used as powder and smallpox scar removal empirically. This research aims todetermine how is the antibacterial potency of the palm sugar ash extract before and after gelformulationagainstStaphylococcusaureusandPropionibacteriumacneswhichcausesacnes.Thisresearch included identificationofpalmsugarashcompound,extractpreparationusingSoxhlet,antibacterial test of extract using diffusion well methods and clindamycin as comparator,formulationofgelpreparationwithcarbopolbase,antibacterial testofgelandevaluationofgelpreparations.Result of this research showed that extractof palm sugar ashbefore andafter gelformulationhadantibacterialactivityagainstStaphylococcusaureusandPropionibacteriumacnes.Atitsminimuminhibitoryconcentrationof0.9%(w/v),extractofpalmsugarashshoweddiameter12.975+0.238mm as inhibition zone againstPropionibacterium acnes. The potential of 1mg ofsoxhletextractisequivalentto0.061mgofclindamycin.Anditsminimuminhibitoryconcentrationof0.5%(w/v)haddiameter9.625+1.428mmasinhibitionzoneagainstS.aureus,Thepotentialof1mgofsoxhletextractisequivalentto0,02mgofclindamycin.Gelpreparationwith20%palmsugarashextractshoweddiameter25.103+0.585mmasinhibitionzoneagainstStaphylococcusaureus,whereasagainstPropionibacteriumacnes,itshoweddiameter24.992+0.718mmasinhibitionzone.Keywords:acnes,antibacterial,ArengapinnataMerr.,gel,palmsugarashextract.


125


FORMULATIONANDANTIBACTERIALACTIVITYSTUDYOFMOUTHWASHCONTAINING

CHITOSANFROMRAJUNGAN(Portunuspelagicus)CARAPACE

PatihulHusni*,DolihGozali,Junaedi

FacultyofPharmacy,UniversitasPadjadjaran,JlRayaBandungSumedangkm21Jatinangor45363*E-mail:[email protected]

ABSTRACT

Dentalplaque,anoralmicrobialbiofilmthatisfoundontoothsurface,playsaveryimportantrolein the process of dental caries and soft tissue inflammation around the tooth.1Chitosan has anantibacterial activity so it is potential to inhibit and prevent dental plaque formation.2Rajungan(Portunuspelagicus)carapaceisoneofthenaturalsourcesofchitosan.3Theaimofthisstudywastodetermine the best formula based on physical stability and hedonic test and to evaluateantibacterialactivityofmouthwashcontainingchitosanfromrajungancarapace.Threeformulasofmouthwashcontainingdifferentconcentrationofchitosan(0.1%,0.2%and0.4%)wereformulatedandevaluatedincludingantibacterialactivity,physicalstabilityandhedonictest.Thecollecteddatawas analized statistically. The study result showed that the mouthwash was a slightly cloudysolution,colorless,sweetandcharacteristicodor.Mouthwashcontaining0.2%and0.4%showedantibacterialactivity.Themosteffectivecontacttimewas90seconds.Organoleptic,pHandviscosityofthemouthwashwerestablefor21daysstorageattemperature27±2˚Cand40±2˚C(75±5%RH).The best formula of the mouthwash was formula containing 0.2% chitosan based on not onlyphysicalstabilityandhedonictestbutalsoantibacterialactivityonStaphylococcusaureuswithanaverageinhibitoryzonediameterof0.7893cmandeffectivecontacttimefor90seconds.Keywords:chitosan,mouthwash,rajungan(Portunuspelagicus),antibacterialactivity


126


RAMBUTANLEAVES(NepheliumlappaceumL.)ETHANOLICEXTRACTANDFRACTIONAGAINSTShigelladysenteriaeATCC13313ANDBacilluscereusATCC1346ASANTIDIARRHEAAGENT

Sulistiyaningsih1,*,AmitaPutriAfin1,ImamAdiWicaksono2

1DepartmentofBiologicalPharmacy,FacultyofPharmacy,UniversitasPadjadjaran,Jatinangor–Indonesia,45363.

2DepartmentofPharmacologyandClinicalPharmacy,FacultyofPharmacy,UniversitasPadjadjaran,Jatinangor–Indonesia,45363.*E-mail:[email protected]

ABSTRACT

Shigelladysenteriae andBacillus cereusarebacterias that cancausediarrhea.Aims this studyisdeterminetheantibacterialactivityofRambutanleavesethanolextractandfractiontoinhibitsS.dysenteriaeandB.cereusgrowthdonebyinvitromethod.Theethanolextractandfractionobtainedfrom the Rambutan leaves were studied for antibacterial activity against S. dysenteriaeand B.cereususingbytheagardiffusionmethod.ThesimpliciaofRambutan leaveswasextractedusingmaceration method. The phytochemical screening was taken using standard methods. TheMinimumInhibitoryConcentration(MIC)andMinimumBactericideConcentration(MBC)testwereperformedbyamacrodilutionmethodandfollowingbysubculturingtheovernightresultontothesurfaceofagarmedia.PhytochemicalscreeningofRambutan leavesethanolextractandfractionrevealed the presence of flavonoids, polyphenols, tannins and saponins, and had antibacterialactivityagainstS.dysenteriaeandB.cereus,whichtheethylacetatefractionwasthemostactivefraction.ThevalueofMICandMBCextractforS.dysentriaewasintherangeconcentration2,5-5,0% w/v, and for B.cereuswas in the range concentration 0,08-0,16% w/v, while for the ethylacetate fraction for S.dysenteriaewas in the range concentration 1,25-2,50% w/v, while forB.cereuswas in the range concentration 0,04-0,08%w/v. The Rambutan leaves gave potent anddirectantibacterialeffectonS.dysenteriaeandB.cereusKeywords:Antibacterial,Diarrhea,Nepheliumlappaceum,Shigelladysenteriae,Bacilluscereus


127

PosterPresentation [PP-21]ANTIOXIDANTACTIVITYFROMETHANOLEXTRACTANDFRACTIONHEMIGRAPHISCOLORATA[1]

Hall.F.LEAVESUSINGDPPH

IrmaErikaHerawati*,HestyNuurHanifah

DepartementofPharmacy,FMIPA,UniversityofAlGhifari,JlCisarantenKulon140,Bandung40293,WestJava,Indonesia


ABSTRACTFreeradicaldefinedaschemicalspeciespossesingunpairedelectrons.Thesespeciesresponsibletomanychronic[3]anddegenerativedisease.Themosteffectivecompoundtoeliminatefreeradicalsareantioxidants.OneofthepotentialnaturalantioxidantalternativesisHemigraphiscolorataHall.F.leaves,whichhasthechemicalcontentofflavonoids,tannins,steroids,terpenoids,potassium,andsaponins.TheaimofthestudywastodeterminetheantioxidantactivityHemigraphiscolorataleaves with method 1 , 1-diphenyl-2-pikrilhidrazil (DPPH). The research stages consist of plantdetermination, macroscopic and microscopic examination, determination of water content,extraction,fractionationanddeterminationofantioxidantactivityagainstDPPHfreeradicals.TheresultsshowedIC50valuesofethylacetatefraction,n-hexanefraction,waterfractionandethanolextractrespectively98.52ppm;140.41ppm;218.4ppm;and374.3ppm.EthylacetatefractionofHemigraphiscolorataleaveswasastrongcategoryforantioxidantactivity.

Keywords:antioxidant,Hemigraphiscolorataleaves,DPPH


128


ANTIBIOTICPOTENTIALOFTAPAKDARA(Catharanthusroseus(L.)G.Don)LEAF’SETHANOLEXTRACTTOINHIBITStreptococcuspyogenes

NitaArtiningsihSayekti*,MuhammadAlanMaulana,PajriahNurhasanah,Triastinurmiatiningsih

DepartmentofBiology,FacultyofMathematicsandNaturalScience,PakuanUniversity,BogorPakuanPOBOXStreetNumb.452,Bogor.16143.Phone:0251-8312206.Fax:0251-8356927


ABSTRACTHemolyticanemiaisaninfectioncausedbyStreptococcuspyogenesactivities,thatcandisturbingcardiovascularsystemandtriggers theautoimmune.Amikacincouldto inhibit theStreptococcuspyogenes’sgrowth,butinlong-termusedwilldisturbingtheperformanceofkidneysandoverdoses.Thatissuescausedthisresearchisrequiredtofindthealternativeherbalantibioticthatovercomeshemolyticanemia.Tapakdara(Catharanthusroseus(L.)G.Don)leafaredrugsformanydiseases,antidote, and antibacterial. That potential causedbyhighly alkaloid, flavonoid, saponin, steroid,terpenoid, and tannin contents. The purposes of this research are to find the optimumconcentrationof tapakdara leaf’s ethanol extract that can inhibit the Streptococcuspyogenes’sgrowthandtoknownthecompoundscontentoftapakdaraleaf’sethanolextract.Themethodsthatusedareinstrumentsandsubstancespreparation,sampledetermination,extraction,phytochemicaltest, make the extract concentration, make the red blood agar, subculture the Streptococcuspyogenes,potentialantibioticoftapakdaraleaf’sethanolextracttest,andstatisticanalysisusedANOVAα=0.05with10treatmentsand5repetitionforeachconcentration.Basedontheresearch,tapakdara’sleafethanolextractcanbealternativeherbalantibiotic.Keywords:Antibiotic,TapakDara,Streptococcuspyogenes


129


ANTIFUNGALACTIVITYSARGASSUMCRASSIFOLIUMANDSARGASSUMPOLYCYCTUMAGAINSTCANDIDAALBICANS

OomKomala*,Triastinurmiatiningsih,RinaYulianti

DepartmentofBiology,FacultyofMathematicsandNaturalSciences,PakuanUniversity,Bogor,

IndonesiaJalanPakuanP.OBox452Telp/Fax.(0251)8375547Bogor*E-mail:[email protected]

ABSTRACT

Sargassum crassifoliumandSargassum polycystumare brown algae species and contain activecompounds such as flavonoids, alkaloids, saponins, phenols, and triterpenoids that act asantibacterial, antiviral and antifungal. of study was to determine antifungal activity ofSargassumcrassifolium andSargassum polycystum againstCandida albicans.Antifungal activityofSargassumcrassifoliumandSargassumpolycystumextractwereexaminedusingthediscdiffusionmethodswithconcentrationsof250mg/mland500mg/mlcomparedtoKetoconazole50µg/mlagainstCandida albicansthroughmeasurementof the inhibition zone. Qualitative phytochemicalteststo know compound ofalkaloids, triterpenoid, saponin, flavonoid, and tannin. 500 mg/mlSargassum crassifoliumand Sargassum polycystumexhibited promising fungistatic agent againstCandidaalbicanswithdiameterofinhibitionzonei.e22mmforSargassumcrassifoliumand21.6mmSargassumpolycystum,andthe its power of the antifungalis lower than Ketoconazole50 µg/ml.Sargassum crassifoliumand Sargassum polycystum contained triterpenoids, saponins, andflavonoidsbioactivecompounds.96%ofethanolextractofSargassumcrassifoliumandSargassumpolycystum had antifungal activity againstCandida albicans. Antifungal effectSargassumcrassifolium (22mm) not real different with Sargassum polycystum (21.6mm) againstCandidaalbicans.Keywords:Antifungal,Candidaalbicans,Sargassum


130


INHIBITORYACTIVITYOFTHELEAFETHANOLICEXTRACTANDFRACTIONSOFHIBISCUSSURATTENSISLON

DIPEPTIDYLPEPTIDASEIV

Yuliet*,ElinYulinahS,IKetutAdnyana

SchoolofPharmacy,BandungInstituteofTechnology,Bandung,Indonesia[3]40132*E-mail:[email protected]

ABSTRACTDipeptidylpeptidase-IV(DPP-IV)inhibitorsareincretinenhancersusedasanewtherapeutictargetforthetreatmentoftype2diabetesmellitus(DM).HibiscussurattensisL(tamoenju)isatraditionalmedicinalplantusedforthetreatmentofdiabeteswithunknownmechanismsofactionbyethnicSumari (Central Sulawesi). The present study aimed to evaluate the inhibitory activity of H.surattensisLleavesethanolicextractanditsfractionsondipeptidylpeptidaseIV(DPPIV)enzymatic,astherapeutictargetsoftype2diabetes.Simpliciawasextractedbymacerationwith96%ethanol.Furthermorefractionatedbyusingliquid-liquidextractionusingasolventofn-hexane,ethylacetate,andwater.TheinhibitoryactivityofethanolicextractandfractionsofH.surattensisLleavesofDPP-IVwereinvestigatedusinginvitroenzymeassaysandspectrophotometricmethod.TheassaywascarriedoutusingtheGPPN(Gly-Pro-p-nitroanilide)substrate.Thep-nitroanilineyieldedfromthereactionbetweenenzyme-substratewithorwithoutthepresenceofinhibitorswasobservedusingaMicroplate reader at awavelength of 405 nm. Sitagliptinwas used as the reference standardinhibitorydrug.IC50ofDPP-IVinhibitoryactivityofethanolicextract,n-hexanefraction,ethylacetatefractionandwaterfractionofH.surattensisLwere236.023±24.161;>1000;17.947±4.842;and847.897±54.425µg/mL,respectively.TheethylacetatefractionshowedthemostpotentinhibitorcomparedthanethanolicextractandtheotherfractionbutstilllowerthanSitagliptin(IC500.020±0.001µg/mL).TheethylacetatefractionofH.surattensisLleaveshaveantidiabeticpropertiesinvitroandarecandidatesforthedevelopmentofnewdrugswiththeinhibitoryactivityofDPP-IVfortype2DMtreatment.Keywords:antidiabeticactivity,DPP-IVinhibitor,fraction,H.surattensisL,leafextract


131


SUBACUTETOXICITYOFCOMBINATIONCURCUMAXANTHORRHIZAAND

AVERRHOABLIMBIONLIVEROFMALEWISTARRATS(RattusnorvegicusBerkenhout)

KartiawatiAlipin*,ElshaPrastiwi,DesakMadeMalini

DepartmentofBiology,FacultyofMathematicsandNaturalSciences,PadjadjaranUniversity.Jl.RayaBandung-SumedangKm.21Jatinangor,Sumedang45363,

WestJava,Indonesia.*E-mail:[email protected]

ABSTRACT

Examination of subacute toxicity is carried out to observe the short-term toxic effects of acompoundonlivingorganismsandotherbiologicalsystems.CombinationofCurcumaxanthorrhizaandAverrhoablimbiextractknowneffectiveindecreasedbloodglucoselevels.ThisstudyaimstodeterminetheeffectofthecombinationofC.xanthorrhizaandA.blimbionhistopathologyofliverofmaleWistarrats.Thisstudywasconductedexperimentallyusingacompleterandomizeddesign(CRD)consistingofsixtreatmentswithfourreplicationsasfollows,NC(negativecontrol):distilledwater; PC (positive control): Herbadrink 16 g/kgBW; and combination of juice (TBW) includingTBW1:20g/kgBW);TBW2:15g/kgBW);TBW3:10g/kgBW),TBW4:5g/kgBW.Treatmentwasgivenorallyfor28days.TheresultsshowedthatallthedosageswerenotsignificantlydifferentwithNCbut different from PC on degeneration cells and necrotic cells. In conclusion that the juice ofCurcumaxanthorrizaandAverrhoablimbihasnosubacutetoxiceffectonhistopathologyliverofmaleWistarrats.

Keywords:Averrhoablimbi,Curcumaxanthorriza,histopathology,liver,subacutetoxicity


132


ANALYTICALMETHODVALIDATIONOFAZADIRACHTININTHENEEMOILCREAMUSING

HPLC

BellaPuteriIrinda*,PatihulHusni,FebrinaAmeliaSaputri,andNoriscaAlizaPutriana

FacultyofPharmacy,PadjadjaranUniversity,Bandung-SumedangKm21Jatinangor

45363Tel./Fax.(022)7796200*E-mail:[email protected]

ABSTRACT

Azadirachtinisacompoundfoundinneemplantthathavethepotentialasantiscabies.Azadirachtinispresentinalmostallpartsoftheplant,includingintheformofoil.Extractingofazadirachtininneemoilneedstobedevelopedinseveralmethodstofindoutthebestmethodtoappealingtheazadiractine.Thisstudywasconductedtofindmethodsthatcanbeusedtodeterminethelevelsofazadirachtininthecream.Themethodusedisbycentrifugation,sonicationandvortexmethods.Thesolventsusedareacetonitrileandethanol.Allmethodsusingacetonitrileassolventsproducetoo large absorbance and the smallest concentration does not appear peak for azadirachtin.Whereasusingthecombinationmethodofsonicationandcentrifugationandsolventethanol,ataconcentrationof2ppm,1ppm,and0.5ppmshowedapeakwhichissuspectedtobeazadirachtinat awavelengthof 208nm. In theoptimization stagewith themobilephaseacetonitrile:water30:70,flowrate1ml/min,wavelength219nm,concentration5ppm,andinjectionvolume20μlfoundpeakonchromatogramthatsuspectedasazadirachtinwithretentiontimeat3.0minutes.

Keywords:Azadirachtin,cream,HPLC.


133


DEVELOPINGNEEM(AzadirachtaindicaA.Juss)OILCREAMFORMULAASANTI-SCABIES

NoriscaAlizaPutriana*,PatihulHusni

DepartmentofPharmaceuticsandPharmaceuticalTechnology,FacultyofPharmacy,Universitas

Padjadjaran,JlRayaBandungSumedangkm21JatinangorIndonesia45363*E-mail:[email protected]

ABSTRACT

ScabiesisoneoftheinfectiousskindiseasescausedbySarcoptesscabieimites.InIndonesia,scabiesranksthethirdof12mostcommonskindiseases.Creamcontainingneemoilisoneofthepossiblealternativesbecauseithasbeenusedinanti-parasiticandanti-scabiestreatment.Thisstudyaimstoproduceacreamformulamadeofneemoil(AzadirachtaindicaA.Juss)asanti-scabieswiththebest formula and optimal effectiveness. Variations of neem oil concentration in the creampreparations are made to determine which one is the most effective as anti-scabies. Physicalstabilityofallcreampreparationsisdeterminedbystoringthemfor3monthsat30±2oCand40±2oC 75±5% RH and doing several physical evaluations every week including organoleptic,Homogeneity,pH,creamtypeandviscosity.Irritationtestandeffectivenesstestofanti-scabiesaredoneonrabbits.Thecreamformulasofneemoil5%,10%,20%,and30%hasagoodphysicalstabilityandincludeinthecategoryofnegligibleirritationresponsewithPrimaryIrritationIndex0;0;0.111;and0.333,respectively.Theeffectivenessofneemoil30%creamisbetterthanthatof5%,10%,and20%.However,comparedtopermethrin5%cream,permethrin5%creamisbetterintermofeffectiveness.Itcanbeseenfromitsspeedinrepairingorhealingscabies.Inaverage,neemoil30%cream shows healing signs onDay- 10while the positive control (permethrin 5% cream) showshealingsignsonDay-8.Neemoil30%creamisthebestformulaandthemosteffectivenessasantiscabies.Keywords:AzadirachtaindicaA.Juss,neem,scabies


134


MICROWAVEASSISTEDSYNTHESISOFANDROGRAPHOLIDEDERIVATIVES:ANEWFAMILYOF

ASPARTICPROTEASEINHIBITORS

SandraMegantara1,2,*,DaryonoHadiTjahjono1,RahmanaEmranKartasasmita1,MariaImmaculataIwo1,JuttiLevita2,SlametIbrahim1

1 SchoolofPharmacy,BandungInstituteofTechnology,Jl.Ganesha10Bandung

2 FacultyofPharmacy,UniversitasPadjadjaran,Jl.RayaBandung-SumedangKm21,Jatinangor,WestJava,Indonesia45363


ABSTRACTAndrographolide,amaincompoundofAndrographispaniculatawassubjectedtosemi-syntheticstudiesleadingtoaseriesofnewderivatives,anovelfamilyofasparticproteaseinhibitors.3,19-2-hydroxybenzylideneandrographolide(3),3,19-3-hydroxybenzylideneandrographolide(4),and3,19-4-hydroxybenzylidene andrographolide (5), the three derivatives of andrographolide havebeen successfully synthesized by reacting andrographolide (1) with hydroxybenzaldehyde (2)undermicrowaveirradiation.Thestructureofnewcompoundswerecharacterizedby1H-NMR,13C-NMR,MSandelementalanalysis.Andrographolideandallsynthesizedcompoundswereanalyzedusingmoleculardockingmethod todetermine its activity as aspartic protease inhibitors in theactivesiteofHIV-1protease,plasmepsin I, II,and IV.Theresult showedthatallnewderivatedcompounds had better activity as aspartic protease inhibitors than andrographolide as leadcompound.

Keywords:andrographolidederivatives,asparticproteaseinhibitors,HIV-1protease,microwave-assistedsynthesis,plasmepsin


135


TABLETANTIBACTERIALTESTBASEDONPadinaaustralisONTOTALBACTERIACAUSING

DIARRHAEMALERAT(Rattusnorvegicus)SPRAGUEDAWLEY

Triastinurmiatiningsih*,TriSaptariHaryani,OomKomala,FaisalDarajat

DepartmentOfBiology,FacultyofMathematicsandNaturalSciences,PakuanUniversityJl.PakuanPOBOX452Bogor,16610,Indonesia


ABSTRACT

Padina australis is one of the seaweed that have a potential as antibacterial Escherichia coli.Triterpenoidsandsteroidsaresecondarymetabolitescompoundscontainedinseaweed,andhasactivitiesasbactericide.AimsofstudywastoinvestigateantibacterialactivityofPadinaaustralistablets against digestive organs bacteria ofmalewhite rats (Rattus norvegicus) SpragueDawleystrainsthatexperienceddiarrhea.ThetestanimalsusedwerewhitemaleratsSpraguedawley9weeks oldweighted between 200 – 300g. The animalswere grouped into 5 treatments, that isnegative control (aquadest), positive control (tabletmetronidazole), and formula with differentdose to which P1 (1.89mg/200 g BB), P2 (3.78mg/200 g BB), and P3 (7.56mg/200 g BB). Thetreatmentwas 1mL samplewith interval of 6 hours a day for 3 consecutive days. The bacterialcolonies waere calculated using Total Plate Count (TPC) method. The results showed that theadministrationofP.australisextracttabletssuspensiondecreasethefrequencyofdiarrheaandthenumberofbacterialcoloniesinthefaecesofSpragueDawleymalewhiterats.ThemosteffectivedosagesuspensionofP.australistabletisthedoseinP3treatmentwhichwas7.56mg/200gBB.Keywords:Tablet,antibacterial,Padinaaustralis,SpragueDawley


136


BRINESHRIMPLETHALITYBIOASSAYANDPHYTOCHEMICALSCREENINGOFPRIMATES-

CONSUMEDPLANTSFROMPANGANDARANBEACHCONSERVATIONAREA

FerryFerdiansyahSofian1*,AmiTjitraresmi1,DudiRunadi1,YulienRatuKania2,AnasSubarnas3

1NaturalProductPharmacyLaboratory,DepartmentofBiologicalPharmacy,FacultyofPharmacy,UniversitasPadjadjaran,Jatinangor-45363,INDONESIA

2ApothecaryProfessionStudent,FacultyofPharmacy,UniversitasPadjadjaran,Jatinangor-45363,INDONESIA

3DepartmentofPharmacologyandClinicalPharmacy,FacultyofPharmacy,UniversitasPadjadjaran,Jatinangor-45363,INDONESIA*E-mail:[email protected]

ABSTRACT

TheareaofnaturereservesinIndonesia,especiallyinPangandaran,isanareathathasgeologicalelementswherelocalpeopleareinvitedtoparticipateinprotectingandimprovingthefunctionofnaturalheritage.Inthisarea,thereisanaturalpotentialthatcanbeusedasasourceofmedicaltreatments[1].Inthisstudy,weinvestigatedthecytotoxicityactivityofethanolextractsofprimates-consumed plants, including Dysoxylum caulostachyum, Eugenia aquea, Garcinia celebica, andPsychotria valetoniicollected from Pangandaran beach conservation area using brine shrimplethalitybioassay.Then,thecytotoxicityactivityofthefractionsfromthemostactiveextractwasalsoinvestigated.Furthermore,thephytochemicalscreeningtestofethanolextractswasexecutedtodetectthepresenceofgroupsofsecondarymetabolitecompoundscontainedintheextracts.Thebrine shrimp lethality bioassay was conducted for investigating the cytotoxicity activity. ThebioassaywasevaluatedusingthestatisticalmethodofPROBITanalysiswiththeaidoftheSPSS23computationpackage inorder todetermine the valueof IC50. Thephytochemical screeningwasexecutedbyusingestablish[2]method.Asresults,study[3]ofbrineshrimptestofethanolextractsofD.caulostachyum,G.celebicaandP.valetoniileavesshowedtoxicityactivitywiththeLC50valuesof 34.14, 705.09 and 586.50 μg/mL, respectively. The ethanol extract of E. aquealeaf was notperformedcytotoxicactivitywhichwasindicatedbytheLC50values>1000μg/mL.Furthermore,thefractionsofn-hexane,ethylacetateandwaterfromD.caulostachyumextracthadthecytotoxicactivitywiththeLC50valuesof12.46,83.59and478.18μg/mL,respectively.Basedontheresultsofphytochemical[4]screeningtest,secondarymetabolitecompoundscontainedineachextractwereflavonoid, quinone, phenol, monoterpenoid and sesquiterpenoid.Asconclusion[5] , the ethanolextract ofD. caulostachyumleaf revealedmore toxic properties than the ethanol extracts ofG.celebicaandP.valetoniileaves.Moreover,theextractofE.aquealeafwasnotperformedcytotoxicactivity. The cytotoxic activity revealed by the fractions from D. caulostachyumextract clearlyindicatedthetoxicproperty.Keywords : Brine shrimp lethality bioassay, Cytotoxicity, Phytochemical screening, Primates-consumedplants


137


SUNSCREENACTIVITYTESTINGOFROBUSTACOFFEE(COFFEACENEPHORAEXFROEHNER)LEAVE

EXTRACTANDFRACTIONS

KikiMulkiyaY*,EstiR.Sadiyah,RiskiSolehati,AldiElgiawan

PharmacyDepartment,FacultyofMathematicsandNaturalSciences,UniversitasIslamBandung

Jl.Ranggagadingno.8,Bandung40116*E-mail:[email protected]

ABSTRACT

Coffeeleavesarepartofaplantthathasnotbeenoptimallyutilized.Thepurposeofthisstudywastoexplorethepotentialuseofcoffeeleavestohelpmaintainskinhealth.Extractionmethodwascarriedoutwithmacerationmethodusing70%ethanolsolvent.FractionationprocesswascarriedoutbyLiquid-LiquidExtractionmethod,usingdifferentsolventstoaccumulatethen-hexane,ethylacetate[1]andmethanolfractions.Activitytestingwascarriedoutonallsamplesatconcentrationsof 50, 100 and 150 ppm includingmeasurements of Sun Protecting Factor (SPF), Erythema andPigmentationTransmittancePercentages(%Te[2]and%Tp[3])usingUV-visiblespectrophotometryat 290 and 400 nm wavelengths. The results showed that the ethyl acetate [4] fraction at aconcentrationof150ppmhadahigherpotentialasasunscreenthanothersampleswithSPFvalueof19.82;%Te[5]10.96and%Tp[6]18.14andincludedinthecategoryoffasttanningsunscreen.Keywords:coffeeleaves,sunscreen,sunprotectingfactor,erythema,pigmentation


138


VirtualScreeningNaturalCompoundsfromPlantsasInhibitorofEstrogenReceptorAlphaI(ESR1)

AndiTrihadiKusumaˡ,*,DaryonoHadi²

ˡDepartementChemical,PharmacyFacultyMoeslemUniversityofIndonesia²DepartementChemical,SchoolofPharmacyBandungInstituteofTechnology

Adress:Jl.UripSumohardjoKm.590231Makassar*E-mail:[email protected]

ABSTRACT

Flavanoidisoneofthechemicalcompoundsfoundinplants.Isoflavones(1.2-diarylpropane)arethelargestgroupintheflavanoidthatcanbeusedtostudytheanticanceractivity.2IOKisoneoftargetinhibitorswhich is obtained fromnatural product compounds.Thepurposeof the research is todesign new compounds have anti-cancer activity, especially for breast cancer based on theevaluation of the affinity of the compound against Estrogen Receptor Alpha (ESR1) using thecomputationalmethod.Theparentcompoundistheteststructureofisoflavoneswhichhasbeenreported to have anticancer activity, particularly for breast cancer. Based on the results of thevalidation method of docking with several combinations, the best method found was Trianglematcher-AffinitydGwithanRMSDof1.0452.Furthermore,thismodelprovidesROCgraphvalueof0.863.Therefore,themethodwasusedtoscreencompoundsintheUIdatabase.Threecompoundswere obtained from the process, which are potentially active against Estrogen Receptor Alpha(ESR1), namely C00010051, C00026048, C00025295. The MD simulations of protein-ligandcomplexesindicatedexchangesprocess,namelytheabsenceofinteractionbetweentheligandwiththe Phe404, instead of the ligand formed hydrogen bonding with Glu353. Meanwhile, theC00025295compoundformedhydrogenbondingwiththeLeu346residue.Keywords:virtualscreening,moleculardocking,moleculardynamic,ESR1


139


TyrosinaseInhibitorActivityMeasurementofCrudeandPurifiedExtractofMoringaLeaves

(MoringaoleiferaL.)

ZainalAbidin*,UmmuKhaeriah,Zuhrina,MamatPratama,MuzakkirBaits

UniversitasMuslimIndonesia,Makassar,90231*E-mail:[email protected]

ABSTRACT

Moringaleavesisoneoftheplantsthathavestrongantioxidantactivity(1).Thecompoundsthathavestrong antioxidant activity also have strong antityrosinase activity(2). Moringaleaves containflavonoidcompoundsthatcaninhibittyrosinaseactivityinthemelaninformationprocess(3,4).Thisstudyaimedtodeterminetyrosinaseinhibitoractivityofcrudeandpurifiedextractofmoringaleaves(MoringaoleiferaL.).Tyrosinaseinhibitoryactivitymeasurementwasdonebyinvitrostudieswithmeasuringdopachromefromtheoxidationofl-tyrosineinthemechanismofmelanogenesis(5).Thecrude extract obtained by usemaceration extractionmethod and ethanol solvent,whereas thepurified extract obtained by use liquid-liquid extractionmethod and ethylacetate solvent(6). TheresultsshowedthathydroquinonehasIC50Valueof18,234μg/mLwhilecrudeextractofmoringaleavesshowsIC50valueof4.405,24μg/mLandpurifiedextractofmoringaleavesshowsIC50valueof401,6228µg/mL.Purifiedextractofmoringaleavesmoreactiveastyrosinaseinhibitorthanitscrudeextractbutlessactivethanhydroquinone.Keywords:Purifiedextract,TyrosinaseInhibitor,UVRays,Merunggaileaves


140


STABILITYOFVITAMINCUNDERINFLUENCEOFRUTINADDITIONASANTIOXIDANT

WinasihRachmawati1,2,*,RahmanaE.Kartasasmita1,SophiDamayanti1,TriSuciati1

1SchoolofPharmacy,InstitutTeknologiBandung,Jl.Ganesha10Bandung,WestJavaIndonesia

2SekolahTingiFarmasiBandung(BandungSchoolofPharmacy),Jl.SoekarnoHattaNo.754Bandung40614WestJavaIndonesia


ABSTRACTVitaminCisunstableandcanbeeasilyoxidizedtoformL-dehydroascorbicinfluencedbycontactwithair,moistureandwater,underexposure to light,andhigh temperature.Additionofwater-solubleantioxidantisacommonmethodtopreventthisreactioninbeverageproducts.TheaimsofthisresearchweretoobtainanalyticalmethodforthedeterminationofvitaminCinthepresenceof rutin and to obtain datawhether the addition of rutin is able to prevent the degradation ofvitamin C under standardized experimental conditions. HPLC method using Phenomenex C18column(3.90x150mm,10µm),UVdetectorat254nmandmethanol–formicacid0.05%(20:80)mobilephasebygradientelutionat1.2mLmin-1of flowratehasbeenapplied.ThestabilitiesofvitaminCinsolutionsunderinfluenceofrutinweretestedatincubationtemperaturesof50and70oCupto2daysfortestsubstancedissolvedinphosphatebufferpH5.4,whilethatwhichdissolvedinmineral-freewaterwasupto16days.Underoptimumcondition, themethodprovided linearcalibrationcurveovertherangeof5.06-13.15µg/mLforvitaminCwitharegressionequationofy=36161.72x–96094andregressioncoefficientof r=0.9992.The limitofdetectionand limitofquantificationforvitaminCwere0.75and1.83µg/mLrespectively.Theintra-dayrelativestandarddeviation(RSD)ofthemethodwere0.595,0.211and0.349%respectivelyandinter-dayRSDwas0.43 %. The recovery for vitamin C in sample simulation was in a range of 98.29 - 103.92 %.Independent of incubation temperatures, only slight inhibition of vitamin C degradation wasobservedfromthe0to240thhoursinthecaseofvitaminCinthepresenceofrutin(10:1)dissolvedinmineral-freewater and incubated at both 50 and 70 oC. The rate of degradation occurred atsecondorderforvitaminCandfirstorderforvitaminCwithadditionofrutin(10:1).ThemethodissuitableforthedeterminationofvitaminCcontainingrutininadmixturewithoutpriorseparation.The overall results of stability test revealed that the addition of rutinwas failed to prevent thedegradationofvitaminCundertheseexperimentalconditions.Keywords:antioxidant,HPLC,rutin,stability,vitaminC


141


FORMULATIONANDCHARACTERIZATIONOFSELFNANOEMULSIFYINGDRUGDELIVERYSYSTEM

(SNEDDS)OFFENOFIBRICACID

WiraNovianaSuhery1,2.*,YeyetCahyatiSumirtapura1,JessieSofiaPramudji1,DikyMudakhir1

1SchoolofPharmacy,BandungInstituteofTechnology,GaneshaStreetNo.10,Bandungcity,West

Java,40132,Indonesia2STIFARRiau,KambojaStreet,SimpangBaru,Pekanbarucity,Riau,28293,Indonesia


ABSTRACTSelf nanoemulsifying drug delivery system (SNEDDS) recently been enhanced to obtain bettersolubility, dissolution and bioavailability of poorly water soluble drugs (BCS II and IV)1,2. Thephysicochemical properties, drug solubilization capacity and the physicochemical nature andconcentrationofoilyphase,surfactantandcosurfactantconsiderablygoverntheselectionoftheSNEDDScomponents3.This studyaimswas to formulate fenofibricacid in the formof lipidbaseformulationandevaluatedthecharacteristicofthedosageform.Theselectionofoil,surfactantandcosurfactant for preliminary screening of self nanoemulsifying formulation was determined bysolubilitystudyoffenofibricacidinvariousvehicles.Thecompositionofselectedoil,surfactantandcosurfactants respectively are medium chain triglyceride (Kollisolv MCT 70®), polyoxyl 40hydrogenated castor oil (Kolliphor RH 40®) and Transcutol P®. Ternary phase diagrams wereconstructedtoidentifytheefficientnanoemulsificationregions.Selectedformulationsfromphasediagramwere evaluated for various parameters such as percent of transmittance, droplet size,thermodynamic stability and drug recovery. Four formulations containing Kollisolv MCT 70®),polyoxyl40hydrogenatedcastoroil(KolliphorRH40®)andTranscutolP®ataratioof10:80:10(F1),20:60:20(F2),20:70;10(F3)and30:60:10(F4)wereselectedtobeevaluatefurther. Theresults show that thebest formulawasF1with thedroplet sizewas 36.30±0.99nm,99,63%transmittance,thermodinamicallystableanddrugrecoverywas90.54%Keywords:Selfnanoemulsifyingdrugdeliverysystem,SNEDDS,fenofibricacid, characterization


142


COSTEFFECTIVENESSANALYSISOFARIPIPRAZOLEANDESCITALOPRAMWITHARIPIPRAZOLE

ANDAGOMELATINEINBIPOLARDISORDERPATIENTSINAPHARMACYINBANDUNG

YuliaWardati*,DesiKristianIndrawati

UniversitasAl-GhifariBandung,CisarantenKulon140th*E-mail:[email protected]

ABSTRACT

AccordingtoWHOin2016,thereare60millionpeopleaffectedbybipolarintheworld,thiscasecontinuestoincrease,includinginIndonesia.Theprevalenceofbipolardisordervariesbetween1-4percentinIndonesia.AtapharmacylocatedinthecityofBandung,whereapsychiatristpractices,thiscase ranks first inavarietyofothermentaldisorders,namely34.1%(in2016).Aripiprazole,escitalopramandagomelatinearedifferentclasses,aripiprazoleisagroupofatypicalantipsychotics,escitalopramisagroupofSNRIs(SerotoninNorepinephrineReuptakeInhibitors)whileagomelatineisametallonergicgroup.Thepurposeofthestudycomparedthetwoalternativesbasedonoutcome(Montgomery Asberg Depression Rating Scale/MADRS reduction) and costs between the twoalternatives. This study was conducted retrospectively in the January-December 2016 of 90continuousphasebipolardisorderpatients,usingtheperspectiveofpatientsandpharmacies,withdirectcostcomponents.ThepharmacoeconomicmethodusedwasaCostEffectivenessAnalysis.Fromthestatisticaltestsperformedontheoutcomegroup,thep-valueobtainedwas0.293.BasedonAverageCostEffectivenessRatio(ACER),aripiprazoleandescitalopramwascosteffectivethanaripiprazole and agomelatine (IDR 1,057,705/MADRS score from patients perspective and IDR842,705/MADRSscoresfrominstitutionalperspective).ThenIncrementalCostEffectivenessRatio(ICER)wasIDR470,000/MADRSscorefrompatientsperspectiveandIDR265,499frompharmacyperspective.Itindicatedthataripiprazoleandagomelatinecosteffectivethanofaripiprazoleandescitalopramintwoperspectivebecausetherewasadecreaseincosts/MADRSscore.Thisshouldbeselectediftheresourcewasinsufficient.Keywords:bipolar,aripiprazole,escitalopram,agomelatine


143


ANTIBACTERIALACTIVITYOFM.candidumLEAFEXTRACTAGAINSHUMANPATHOGENS

Titajuwitaningsih1,*,Chairunnisa1,SriAdelilaSari1,andIisSitijahro1

1DepartmentofChemistry,FacultyofMathematicandSciences,UniversitasNegeriMedanWillemIskandarStreet,PasarVMedanEstate,KecamatanMedanTembung,

NorthSumatera,Postalcode20221,Indonesia*E-mail:[email protected]

ABSTRACT

Antibioticsderivedfromnaturalproductsareveryinterestingtoberesearchedbecauseofsomebacteriathathavebeenresistanttoexistingantibiotics.LeavesofMelastomacandidumhavebeenusedintraditionalmedicinetotreatvariousdiseases.Thisstudyevaluatedtheactivityofacetoneextracts ofM. Candidum against nine pathogenic bacteria. The test of antibacterial activity isconducted in vitro with paper disc diffusion method with M02-A11 standard Clinical AndLaboratoryStandardInstitute(CLSI).Theresultofantibacterialactivitythencontinuedwiththedeterminationoftheminimuminhibitoryconcentration(MIC)valueandtheminimumbactericidalconcentration (MBC) with microdillution methods M07-A9 (CLSI). Identification of secondarymetabolite contents determined with 1 D NMR spectroscopy. The results obtained thatM.CandidumleafacetoneextracthasactivityagainstsevenpathogenbacteriawithMICrange625-2500µg/mLandMBCbetween1250-5000µg/mL.M.candidumextractshowedthebestactivityagainstB.cereusATCC1178,E.coliATCC(25922)andS.entericaATCC14028bacteriawithMICvalueof625µg/mL.1DH-NMRdatashowedthatM.candidumextractcontainedterpenoidsandaromaticcompounds.M.candidumispotentialsourceofnewantibioticcompoundsKeywords:Melastomacandidum,antibacterial,humanpathogens.


144

PosterPresentation [PP-42]TheProtectiveEffectsofRedDragonFruitJuicetowardsDoxorubicin-inducedTesticularToxicity

inRats.

IndiraSeptianawati*,BayuFebramPrasetyo,VetnizahJuniantito

1DepartementClinical,Reproduction,andPathology,FacultyofVeterinaryMedicine,

BogorAgriculturalUniversity,JlLampusIPBDarmahaBogor*E-mail:[email protected]

ABSTRACT

Doxorubicin(DOX)isananthracyclineantibioticthatiseffectivetowardsvarioustypesofcancer.HowevercontinuousadministrationofDOXresulting intoxicity incertainorgans,oneofthemistestes.ToxicitybyDOXcanresult inanincompleteprocessofspermatogenesis.Theantioxidantslevelinreddragonfruitishigherthantheantioxidantslevelofwhitedragonfruitbecauseoftheredpigment(antocyanidin).Thisstudyaimstodeterminetheprotectiveeffectsofdragonfruitjuiceontheincidenceoftesticulartoxicityduetodoxorubicinapplicationinrats.Thisexperimentused18maleSprague-Dawleyratsandweredividedintothreegroups:negativecontroltreatment(saline),positivecontroltreatment(DOX),andDOX+reddragonfruitjuicetreatment.Testiculartoxicitywasinducedbyintraperitonealinjectionsofdoxorubicinatdoseof4mg/kgbodyweight(BW)onceaweek,andthe juicewasgiventhreetimesadayforfourweeksatdose2ml/500gramBW.Theresultsinthenegativecontrolgroupshowsthenormalmorphologyoftesticularcells,characterizedby spermatogenic cells (spermatozoa, spermatocyte,early spermatid, and late spermatid) in thelumen tubulus seminiferus.Thegroup that isgivenDOX+dragon fruit juice treatment shows thecondition of the seminiferous tubules and the number of spermatogonia, spermatocyte, earlyspermatid,and latespermatid is larger thanthegroupwithpositivecontrol (DOX) treatment. Inconclusion dragon fruit juice may provide protective effects for DOX induced testes that ischaracterizedbyratspermatogeniccells(spermatogonia,spermatocyte,earlyspermatid,andlatespermatid) in the seminiferous tubules better than the group that is only treated by DOX.Conclusively,reddragonfruitjuiceamelioratestesticulartoxicityinducedbydoxorubicin.Keywords:doxorubicin,testiculartoxicity,reddragonfruitjuice


145


STUDYOFMOLECULARDOCKING,MOLECULARDYNAMICANDTOXICITYPREDICTIONOF

SEVERALQUINOLINEALKALOIDDERIVATIVESASABRUTONTYROSINKINASEINHIBITORASANTILEUKEMIA

FauzanZeinMuttaqin1,*,IkmaHanifah1,HubbiNasrullahMuhammad1,2

1SekolahTinggiFarmasiBandung(BandungSchoolofPharmacy)Jl.SoekarnoHattaNo.754Cibiru,

Bandung406172SchoolofPharmacy,InstitutTeknologiBandung


ABSTRACTLeukemia causes 333,975 deaths per year in Indonesia. Some quinoline alkaloids have a similarchemicalstructuretoB-cellsignalinginhibitorthatleadtoBrutonTyrosineKinase(BTK)inhibitionwhich can be used in leukemia treatment. This research aims to study the interaction of somequinolinealkaloidstowardsBTKandtopredictthetoxicitytoensuretheirsafety.Thisstudywascarried out using computational studies, including molecular docking and molecular dynamicssimulation,andtoxicitypredictiontoassessthecompound’sactivitytowardsBTKandtheirtoxicity.Moleculardockingsimulationsshowedthat10compounds(S1,S2,S4,S5,S8,S13,S14,S16,S17andS20)hadthebestaffinitytoBTK.Moleculardynamicssimulationstothese10compoundsshowedthatonly7compounds(S1,S5,S8,S13,S16,S17,andS20)couldstabilizetheinteractiontowardsBTKwithRMSDandRMSFvalueof0.5±2Åand0.5±6,5Årespectively.Toxicitypredictionresultsshowedthatthesequinolinealkaloidshadavarioustoxicitycharacteristicsbutmostofthemwerenotcarcinogensandmutagens(S4,S5,S6,S7,S8,S10,S11,S12,S14andS15).ItcanbeconcludedthatYukositrin(S8)hasthemostpotentialaffinitytowardsBTKwhichcanbeusedasantileukemiawithalowtoxicity.Keywords:antileukemia,quinolinealkaloids,BrutonTyrosineKinase

PosterPresentation


146

[PP-44]

TotalMonomericAnthocyaninPigmentContentinEthanolandMethanolButterflyPeaFlower

(ClitoriaternateaL.)ExtractbypHDifferentialMethod

AnneYuliantini*,WinasihRahmawati,AyuApriliani

SekolahTinggiFarmasiBandung,JlSoekarnoHattaNo754CibiruBandung,Indonesia*E-mail:[email protected]

ABSTRACT

Butterflypeaflower(ClitoriaternateaL.)isoneofflowerthatcontainsblueanthocyanin.Thisstudyaims to determine total anthocyanin content in ethanol andmethanol extracts of butterfly peaflower by a pH differential method. The method can be used for the determination of totalmonomericanthocyanincontent,basedonthestructuralchangeoftheanthocyaninchromophorebetweenpH1.0and4.5withmeasuringthedifferenceinabsorbanceatmaximumwavelength(510nm)which is proportional to the concentration of anthocyanin1. Extraction of flower donewithmacerationmethodusingethanolandmethanolasasolventfor24hoursindarkconditionwiththeratio flower and solvent of 1: 4. The anthocyanin in extract monitored by Thin LayerChromatographywithbutanol,aceticacid,andwater(4:4:2)aseluenttoconfirmanthocyanintype2.TheResultshowedthatnosignificantlydifferenceoftotalanthocyanincontentbetweenethanoland methanol of butterfly pea flower extract, they are 16.858± 2.053 and 18.269± 2.455,respectively.ThechromatogramresultshowedyellowspotaftersprayedAlCl3inethanolandoneofspothadRf0,71-0,73whichshowedflavonoidcompoundandoneofanthocyanintypeinbutterflypeaextractisdelphinidin2.Keywords:totalanthocyanincontent,pHdifferentialmethod,extract,ClitoriaternateaLPosterPresentation


147

[PP-45]

CARICAPAPAYALEAVESREDUCEDBLOODPRESSUREANDARTERIALSTIFFNESSONANIMALMODELHYPERTENSIONINDUCEDBYHIGHFRUCTOSEDIET

PatonahHasimun*,AgusSulaeman,IntanDwiPuspitaMaharani

Pharmacologyresearchgroup,BandungSchoolofPharmacy,Sukarno-Hattano754,Bandung,WestJava,Indonesia,40617E-mail:[email protected]

ABSTRACT

Hypertension isdefinedasan increase insystolicand/ordiastolicbloodpressureofmorethan140/90mmHg.Epidemiologicalstudiesshowthatthereisarelationshipbetweenhypertensionandtheincidenceofarterialstiffness,especiallyintheelderly.Uncontrolledhypertensionincreasestheriskofmorbidityandmortality.Bloodpressuremanagementaimstopreventdamagetovarioustargetorganssuchastheheart,brain,kidneys,eyes,andperipheralarteries.Papayaleaves(CaricapapayaL.)havebeenknownempirically to treathypertension.This studyaims todetermine theeffectofpapayaleavesprocessedintonoripreparation,bloodpressureandarterialstiffness.ThisstudywascarriedoutonanimalmodelsofmaleWistar rats inducedhypertension.Atotalof30Wistar ratswere randomlydivided into 6 groups consisting of group1 (receiving drug carriers),group2(receivingcaptopril2.5mg/kg)andel3-5(receivingpapayaleavesmixedwithnori5,10,and20%onthediet).Allgroupsexceptthenormalgroupreceived66%fructoseintheirfoodfor21days.Parametersmeasuredweresystolicbloodpressure,diastolicbloodpressureandpulsewavevelocity(PWV)ondays0and22.Theresultsindicatedthatthegroupreceivednoriof10%papayaleafshowednormalizebothsystolicanddiastolicbloodpressurecomparedtothegroupreceivingcaptopril (p>0.05). The group received nori of 10% papaya leaf showed a decreased in arterialstiffness(decreasesPWVvalue)significantdifferentcomparedtocontrolgroup(p<0.05).Theresultscan be concluded that nori of papaya leaf is effective as an antihypertensive and improves theelasticityofarteries.

Keywords:arterialstiffness,bloodpressure,CaricapapayaL.,hypertension,pulsewavevelocity

PosterPresentation


148

[PP-46]THEINFLUENCEOFKEREHAU(CallicarpalongifoliaLamk.)LEAVESEXTRACTONLIPIDRATIOOF

WISTARMALERAT

ElisSusilawati*,WidhyaAligita,Yeni

DepartmentofPharmacology,BandungSchoolofPharmacyJl.SoekarnoHattaNo754Bandung,Indonesia40614


ABSTRACT

TheethanolextractofKerehauleaves(EEKL)isknowntohaveactivityasanti-inflammatory[1]andantioxidantthatispotentialinpreventingatheroscleroticdisease[2][3].ThisstudywasundertakentodeterminetheabilityofEEKLinloweringlipidlevelsandlipidratio.Thestudywasconductedin-vivousingmalewistarratsinducedwithhighfatandfructose25%feedfor45days[4]thatdividedinto 6 groups (n=3), ie control (-) (CMC-Na 0.5%), control (+) (induction), comparison group(simvastatin 0.9 mg/kgBW), and 3 groups of extract with doses of 75, 150 and 300mg/kgBW.AdministrationofCMC-Na,simvastatin,andEEKLwereperformedatthesametimewithinduction.Theparametersmeasuredweretriglyceride(TG),totalcholesterol(TC),andhighdensitylipoprotein(HDL)cholesterollevelsbeforetreatmentandafter45daysoftreatment.ThestudyresultsshowedthatEEKLatdosesof75mg/kgBWcoulddecreasedlevelsofTC(63.63±11.23), increasedlevelofHDL (30.47±2.78), decreased lipid ratio ie cardiac risk ratio (CRR) (2.09±0.18) and aterogeniccoefficient(AC)(1.09±0.18).ItcouldbeconcludedthatEEKLatadoseof75mg/kgBWhadtheabilityinloweringlipidlevels(TCandHDL)andlipidratio(CRRandAC).Keywords:Kerehauleaves,CallicarpalongifoliaLamk.,lipidratio

PosterPresentation


149

[PP-47]

INFLUENCEOFMULBERRYLEAFEXTRACT(MorusalbaL.)ONDIURETICACTIVITYOFMALEWHITEWISTAR-STRAINRAT

DythaAndriDeswati1,*,SriMaryam1

DepartmentofPharmacy,FMIPA,UniversityofAl-Ghifari,Jl.CisrantenKulonno140,Bandung,WestJava,Indonesia,40293*E-mail:[email protected]

ABSTRACT

TheMulberryLeaf(MorusalbaL.)isatraditionalmedicinalplantthatcanbeusedasadiuretic1.Theaimsofthisresearchweretoknowthediureticeffectofethanolofmulberryleafextract inmalewhiteWistar-strainratsbyusingLipschitzmethodandtoknowoptimaldoseofethanolofmulberryleafextractasdiuretic.Inthisstudy,theratsweregroupedinto5groups,whicheachgroupconsistedof5ratsandthentreated.Thecategorizationofthegroupwere:normalPGAgroup2%,furosemidecompoundgroup3.6mg/kg,dose1groupextract140mg/kg,dose2groupextract240mg/kg,anddose3groupextract420mg/kg.Testingdiureticeffectwasdonebymeasuringthevolumeofurinefor6hours.Theresultshowedthatmulberry leafextract (MorusalbaL.)haddiureticeffect.Theeffectivedoseasadiureticisobtainedatdoseof420mg/kg.Keywords:diuretic;extract;mulberryleaf;MorusalbaL.

PosterPresentation


150

[PP-48]

STUDYOFSOLIDSTATEKINETICSFROMONEOFMETASTABLEEFAVIRENZDUETOTHEGRINDINGPROCESS

YogaWindhuWardhana1,2,*,VeinardiSuendo3,SundaniN.Soewandhi1

1DepartmentofPharmaceutics,SchoolofPharmacy,InstituteTechnologyofBandung(ITB)2DepartmenttofPharmaceuticsandPharmaceuticalsTechnology,FacultyofPharmacy,

UniversitasPadjadjaran(UNPAD)3InorganicandPhysicalChemistryDivision,FacultyofMathematicsandNaturalSciences,

InstituteTechnologyofBandung(ITB)*E-mail:[email protected]

ABSTRACT

Study of solid-state kinetics among drug polymorphs is very limited. Therefore, a lot ofpharmaceutical solid dosage forms preferred to metastable or unstable polymorphs in theirproductstoachievegoodbioavailability.However,theriskofchangingthequalityshouldbeknownbetterintheproducts.Thisinvestigationworkonthesolid-statekineticsobservationfromoneofmetastable efavirenz (EFV). EFV popular known as an anti-HIV type 1 drug has 23 differentpolymorphformsincludeamorphousreported.But,theonlystableformusedinthewidespreadmarket.Theotherpolymorphspropertieshavelackedanyinformationthatshouldcomplete.Itiscommonknowledgethatameta-stableoranunstableformhasmoresolubilitywhichhasbenefitforbioavailability.However,thestabilityofpolymorphictransitionamongpolymorphsshouldbelearnedtokeepitsquality.Oneofthemeta-stableformsaimtostudyinthisresearchwastheβformwhichresultfrommethanolrecrystallization.Treatmentfortriggeredthechangesusedbythegrinding process. The solid-state transition was monitored by Powder X-ray Diffraction (PXRD)method and quantification by Rietveld refinement aid. The observation resulted in thattransformation of β form to I (stable form) on two different mechanisms. There were one-dimensional diffusion and three-dimensional geometry contracted. Theremeant that growth ofchanging came from inside the crystal nucleus as an infinite flat planeand fromoutside also atsurfaceexpandtoalldirection(volumeexpansion).

Keywords:efavirenz,polymorph,solid-statekinetics


151


FORMULATIONANDINVITRORELEASESTUDIESOFANTHOCYANINFROMPURPLESWEET

POTATOHYDROPHILICCREAMS

CokordaIstriSriArisanti*,AnakAgungIstriSriHartaniDewandari,IGustiNgurahJemmyAntonPrasetia

PharmacyDepartment,FacultyofMathematicandNaturalScience,

UdayanaUniversity,KampusBukitJimbaran,80361*E-mail:[email protected]

ABSTRACTThisinvestigationwasaimedtodeveloptopicalcreamcontaininganthocyaninfrompurplesweetpotato. Thehydrophilic creambasewith complexemulsifierwas chosenas thedelivery system.Different concentrations of Virgin Coconut Oil (VCO) were used to enhance the permeation ofanthocyanin.Allthepreparedcreamwereevaluatedforvariouspropertiessuchashomogeneity,viscosity, spreadability, adhesion, anddrug content. In vitropermeation studiesof creamwereperformed by using Franz diffusion cells. All prepared formulations indicated good physicalproperties.Theusedof20%VCOinthecreamformulationshowedthelowestviscosity(3430+0.4cps), higher spreadability (6.10 +0.2 g.cm/sec), but lowest adhesive power (3.50 +0.4 g.cm/sec)compared to other formulation. The percutaneous flux and enhancement concentration ofanthocyaninacrossepidermiswereenhancedbyincreasingoftheVCOtothecream.Thecumulativepercentamountofanthocyaninreleasefromcream(59.49%)within3hours.TheresultofinvitropermeationstudyfollowedtheHiguchimodel,anditisknownthatthemechanismreleaseofdrugwasdiffusionmediated.Keywords:anthocyanin,purplesweetpotato,virgincoconutoil,penetrationenhancer,permeationstudy

PosterPresentation


152

[PP-50]TOTALANTHOCYANINSOFPETALOFREDROSE(RosadamasceneMill.)ANDREDCHINAROSE

(Hibiscusrosa-sinensisL.)FROMMACERATIONANDPERCOLATIONMETHODS

GinayantiHadisoebroto1,*,IrmaErikaHerawati1,NyiMekarSaptarini2

1DepartementofPharmacy,FacultyofPharmacyandNaturalSciences,UniversitasAlGhifari,

JlCisarantenKulon140,Bandung40293,Indonesia2DepartmentofPharmaceuticalAnalysisandMedicinalChemistry,FacultyofPharmacy,UniversitasPadjadjaran,JlRayaBandungSumedangKm21,Jatinangor45363,Indonesia


ABSTRACTThepetalsofaredrose(RosadamasceneMill.)andredChinarose(Hibiscusrosa-sinensisL.)areredbecauseofanthocyanins.Anthocyaninsarewater-solubleplantpigmentswhichcanbeusedasanaturaldyewithantioxidantactivitybecauseofitshydroxylgroups[1].Thisstudyaimedtocomparethe total anthocyanins content of petals of a red rose, and red China rose which extracted bymaceration and percolation. Total anthocyanins analysis was conducted by the pH differentialmethod; pH affects the anthocyanin color through structure alteration [2]. Total anthocyaninscontentofmacerateofredroseandredChinarosewas0.458mg/Land0.181mg/L,respectively.WhattotalanthocyaninscontentofpercolateofredrosesandredChinarosewas0.361mg/Land0.076mg/L,respectively.Itwasconcludedthatthetotalanthocyaninscontentofredrosewashigherthan red China rose andmacerationmethod provide higher total anthocyanins than percolationmethod.Keywords:extractionmethod,pHdifferential,coloralteration

PosterPresentation


153

[PP-52]

FORMULATIONANDOPTIMIZATION[1]OFBISOPROLOLFUMARATEORALLYFASTDISSOLVINGFILMWITHCOMBINATIONOFHPMCE15ANDMALTODEXTRINASMATRIXPOLIMERS

AristhaNovyraPutri1,RahmayantiFitriah1

SekolahTinggiKesehatan(STIKES)BorneoLestariBanjarbaru1

KelapaSawit8StreetBumiBerkat,Banjarbaru–SouthKalimantan*E-mail:[email protected]

ABSTRACT

Bisoprololfumarateisadrugbelongingβblockersgroup,andspecificallyisselectiveβ1adrenergicreceptorblocker,aclassmedicinesusedprimarilyincardiovasculardiseases.Thedrughas9-12hrshalflife(t1/2)andshowsbioavailabilityofmorethan80%1.Anorallyfastdissolvingfilmdosageformwithdrug inmouthareabsorbedthroughbuccal/oralmucosa intosystemiccirculationavoidingfirstpassmetabolism.ThestudyaimedtomadeoforallyfastdissolvingfilmbisoprololfumaratebysolventcastingmethodwithcombinationofHPMCE15andmaltodextrinasmatrix.Factorialdesignwasappliedtooptimizetheformulaoforal ly fastdissolvingfilmbisoprolol fumaratebyvaryinglevelofpolymer,itwasHPMCE15300–600mg,maltodextrin50–150mg,andPEG40050–90mg.Theoptimumformulawasdeterminedbysuperimposedcontourplotfromvariousparameters:physical properties of oral ly fast dissolving film bisoprolol fumarate such as thickness, foldingendurance,surfacepH,invitrodisintegration,hidrationstudy,anddrugreleasefor300[15]secondusingDesignExpert®program.Thestudyresultshowedthatphysicalpropertiesof8formulassuchasorganoleptictestweretransparent,homogeny,andsmoothinbothofside;thickness0.062–0.132mm;weightvariation50.85–70.60mg;foldingendurance987–2012;surfacepH6.69–6.99;disintegration(dropmethod)18.33–20.76sec;disintegration(petridishmethod)27.63–30.41sec;invitrodissolutionfor300[24]secwas94.35–98.99%;andswellingindexupto30secondwas146.63–173.34%.BasedonsuperimposedcontourplotDesignExpert®fromfactorresponseoffoldingendurance,surfacepH,invitrodisintegrationdropmethod,invitrodisintegrationpetridishmethod,swellingindex,danDE300withastatisticallysignificantfindingrequiresaP-valueof0.05were obtained[28] optimum formulas for the area in the range of HPMC E15 509.88 mg;Maltodextrin108.63mg;andPEG40050mgwiththevalueofdesirability0.519.Keywords:bisoprololfumarate,orallyfastdissolvingfilm,factorialdesignPosterPresentation


154

[PP-53]

HUMANREDBLOODCELLSMEMBRANSTABILITYPROPERTIESOFZingiberottensiiVal.EXTRACTSLiaMarliani*,EtikaDwiMayasari,NadyaNurrachmayanti,WempiBudiana,ArisSuhardiman

BandungSchoolofPharmacy,Jl.SoekarnoHattaNo.754Bandung


ABSTRACT

The Rhizomes Of Zingiberaceae Plants. Preparative Biochemistry & Biotechnology, 43:60-78.Zingiber ottensiiVal. is a plant of the Zingiberaceae family that has been used for empiricmedicine such as itchy, lumbago, fever and cough1. This plant has been proved as antioxidant,antimicrobial, anticancer and antidiabetes2,3,4. Zingiber ottensii Val. also suggested as anti-inflammatory5. Themembrane stabilizing property of extract onHumanRedBloodCells (HRBC)possessesanti-inflammatoryproperty.Thisstudyaimstodeterminetheanti-inflammatoryactivityofextractofZingiberottensiiVal.leavesandrhizomethroughHRBCmembranestabilitytest.Theextractionwasdonebymacerationmethodusingethanol70%assolvent.ThemembranestabilizingpropertyofextractonHumanRedBloodCells(HRBC)wasdeterminedbyinhibitionofhemolysisthatinducedbyhypotonicsolution.Thisresultwasusedasameasureofanti-inflammatoryactivity,with sodiumdiclofenac as a positive control. The test result of Zingiber ottensii Val. leaves andrhizomeextractshowedthe IC50valuerespectively were341.20μg/mland387.67μg/ml.Thisresultshowedthattheleavesextracthasmoremembranestabilizingpropertythanrhizomeextractwhereas the IC50 value of positive control, sodium diclofenac was 49.01 μg/ml. These resultssuggestedtheextractnotpotentialasanti-inflammatoryagents,butfurtherstudycanbecarriedoutonleavesfraction.Keywords:Anti-inflammation,HRBC,ZingiberottensiiVal.,

PosterPresentation


155

[PP-54]EFFECTOFETHANOLEXTRACTOFArchidendronpauciflorumFRUITPEELONCATALASEENZYMEACTIVITYANDMALONDIALDEHYDECONCENTRATIONINSTREPTOZOTOCIN-INDUCEDDIABETIC

FEMALEWISTARRATS(RattusNorvegicus)DesakMadeMalini*,NiningRatningsih,Madihah,DindaHani’ahArumSaputri,KartiawatiAlipin,

RetnaningtyasSiskaDianty,WawanHermawan

DepartmentofBiology,FacultyofMathematicsandNaturalSciencesUniversitasPadjadjaran.

Jl.RayaBandung-SumedangKm.21JatinangorSumedang45363,WestJava,Indonesia.*E-mail:[email protected]

ABSTRACT

Hyperglycemiaindiabetesmellituscausesanincreasedformationofreactiveoxygenthatleadstooxidative stress1. The fruit peel of jengkol (Archidendronpauciflorum; Fabaceae) is theoneof amedical plant that used by Karangwangi’s villagers, Cianjur, DistrictWest Java to cure Diabetesdisease(Malinietal2017)Thejengkolfruithasknowncontainseveralantioxidantcompounds,i.e.flavonoids,tannins,andpolyphenols.Thisstudyaimedtoinvestigatetheabilityofethanolextractofjengkolfruitpeel(EEJFP)toincreasethecatalaseactivityanddecreasemalondialdehyde(MDA)concentrationinstreptozotocin-induceddiabeticrats.Themethodusedisanexperimentalmethodusingacompletelyrandomizeddesignwith6treatmentsand3replications.RatsareinducedwithStreptozotocindose60mg/kgBWinallgroupsofanimalsexceptNegativeControl(CN).72hoursafter induction, those with blood glucose level>220 mg/dl are divided into five groups. ThetreatmentsgivenareNC(CMCsolution0.5%),PC(CMCsolution0.5%),G(glibenclamide),Treatment1,Treatment2,Treatment3(EEJFPdose385;770;1540mg/kgBW).Treatmentwasgivenorallyfor14days.Catalaseactivityinliverwasmeasuredbyusingspectrophotometrictechniques.Meanwhilein MDA concentration from pancreas and liver tissues were measured by Thiobarbituric AcidReactivesubstance(TBARs)method.TheresultshowedthatthetreatmentofEEJFPatthedoseof770mg/kg BW increased catalase activity of rats liver, although the value was lower than thenegativecontrol.However,EEJFPshowedthathadanIC50valueof6,35ppmwhichwasclassifiedastheverystrongantioxidantandtheextractatadose1540mg/kgBWwaseffectivetodecreaseMDAconcentrationfrompancreasandliverat41.43%and53.1%,respectively,inthestreptozocin-induceddiabeticrat.Inconclusion,EEJFPhaspotencyasapowerfulantioxidantwhichcandecreasepancreaticandlivermalondialdehydeconcentrationandincreasedcatalaseactivityofratsliverinstreptozotocin-induceddiabeticfemaleWistarratsKeywords:catalaseactivity,malondialdehyde,Archidendronpauciflorum

PosterPresentation


156

[PP-55]PATTERNOFANTIRETROVIRAL(ARV)DRUGUSEINOUTPATIENTPRESCRIPTIONSFROM

HIV/AIDSCLINICATONEOFTHEPRIVATEHOSPITALINBANDUNGCITY

AniAnggriani,EsterMandalas,OlgaSusanaWiku

BandungOfSchoolPharmacy(SekolahTinggiFarmasiBandung)

JalanSoekarnoHattaNo754CibiruBandung,Indonesia.*E-mail:[email protected]

ABSTRACT

TheHumanImmunodeficiencyVirus(HIV)continuestobeamajorglobalpublichealthissue,whichtargetsthehumanimmunesystem.TheusingofARVsinthetreatmentofHIV/AIDSincreasedlifeexpectancyforPLHIV(PeopleWithHIV/AIDS).Thisstudyaimstodeterminethedescriptionoftheusing of ARV drugs in outpatients of the HIV / AIDS Clinic and assessed their suitability withestablishedtreatmentstandards.Thisresearchwascarriedoutinadescriptivenon-experimentalmanner,withdatacollectioncarriedoutretrospectively,usedpatientprescriptiondatafromApriltoDecember2017.Theresultsofquantitativestudiesshowed87%weremalepatients,andthelargest age groupwas 20-29 years (39%) . Class of antiretroviral drugs usedwere Nucleoside /Nucleotide Reverse Transcriptase Inhibitors (NRTIs), Non-Nucleoside Reverse TranscriptaseInhibitors(NNRTIs),andProteaseInhibitors(PI),withacombinationofantiretroviraldrugsmostwasthecombinationof first-linetenofovir+ lamivudine+efavirenz(69%)whilethesecond-linedrugzidovudine+lamivudine+lopinavir/ritonavirwas1%.Themostcommonlyusedcomorbiddrugwascotrimoxazole.Forqualitativedata,theaccuracyofcombinationanddoseofARVdrugswas100%inaccordancewithPermenkesNo.87/2014,with79%ofpatientsadheredtoantiretroviraltreatment everymonth. The potential formost ARV drug interactionswith other drugs for themoderate categorywas zidovudin + cotrimoxazole (11%)which occured pharmacokinetically bydecreasingrenalclearanceofzidovudineandglucuronidemetabolites.ThepatternofusedofARVdrugs hadmet the standard of Permenkes No.87/2014, with themost usedwere the first linecombinationoftenofovir+lamivudine+efavirenz.Keywords:Antiretroviral,HIV/AIDS,PatternofDrugUsePosterPresentation


157

[PP-56]

INHIBITORYALFAGLUKOSIDASEACTIVITYASSAYOFLEAVE,FLOWER,ANDRHIZOMEEXTRACTSOFEtlingeraelatior

R.HerniKusriani1,2,*,LiaMarliani1,2,LusianaRizky2

1FacultyofPharmacy,PadjadjaranUniversity,2BandungSchoolofPharmacy,Indonesia


ABSTRACT

Diabeticdiseaseisachronicmetabolicdisorderwhichcharacterizedwithhighbloodglucoselevel.Thehighprevalenceofdiabetesshowed6.9%ofIndonesianpopulationandeveryyaearshasincreased.InthepreviousstudiesitwasknownthatalphaglucosidaseandalphaamylaseinhibitoryactivityonethanolextractofEtlingeraelatiorrhizomewas28.36-99.79%and35.91-58.13%. The research on antidiabetic agents continues to develop, such as through alphaglucosidase inhibition mechanism. Alfa glucosidase enzyme was catabolized carbohydratescomplexpolysaccharidesintomonosaccharides.FlavonoidofEtlingeraelatiorrhizomeshowedtheinhibitionagainstalfaglucosidase.LeavesandflowersextractofEtlingeraelatioralsohavetheflavonoidcontentthatwehopelysotheactivityofalfaglucosidaseinhibitor.Theobjectofthisstudywastodeterminetheinhibitoryactivityofalphaglucosidaseenzymeofleave,flowerandrhizomeextractsofEtlingeraelatior.Eachsimpliciawereextractedbymacerationmethodfor 3x24 hours with ethanol as a solvent. Monitoring of Etlingera elatior compound in achromatography thin layer (TLC)was done. The inhibition activity of alfa glukosidasewithmicroplatereadersatwavelengths405nmwasobservedwithacarboseasastandard.Leave,flower, and rhizome extracts of Etlingera elatior showed inhibitory activity against alfaglucosidase enzymes with IC50value were 165.61 µg/ml; 42.53 µg/ml and 304.44 µg/ml,respectively; while acarbose as standart was 226.55 µg/ml. Flowers and leaves extract ofEtlingeraelatiorshowedalfaglucosidase inhibitoractivitybetterthanacarboseasstandard,andcouldbeapotentiallytodevelopintoanantidiabeticpreparation.Keywords:Diabetesmellitus,alfaglucosidaseinhibitor,acarbose,Etlingeraelatior

PosterPresentation


158

[PP-57]

PROFILEofANTIBIOTICUSEinOUTPATIENTinaHOSPITAL

IdaLisni*,NiNyomanSriMasHartini,AnugrahNurRistinovit

BandungOfSchoolPharmacy(SekolahTinggiFarmasiBandung)

JalanSoekarnoHattaNo754CibiruBandung,Indonesia.*E-mail:[email protected]

ABSTRACT

Antibioticsareonedrugwhichismostoftenusedinhealthfacilitiesbecauseitisthemaintreatmentfor infectious diseases. The high intensity of antibiotics use caused many problems, especiallybacterialresistancetoantibiotics(KemenkesRI,2011).Thisstudyaimedtodescribetheprofileofantibioticuseinoutpatientinahospitalandtodeterminetheappropriateantibioticprescriptionfor the Formulary. This studywas a descriptive non-experimental studywith retrospective datacollection.Thecriteriaforthedrugsinthisstudywereallantibioticgroupsthatusedasprophylaxis,empiric,ordefinitivetherapyintheoutpatientsinahospitalonFebruary2018.Datawascollectedfrom the prescriptions and analyzed to get informations on patient characteristics, profile ofantibiotics use, appropriateness dose of antibiotic prescriptions, potential of antibioticsinteractions,andappropriatenessofantibioticsprescribingtotheformulary.Theresults,therewere755antibioticprescriptions,andCefiximeisthemostprescribed(42.65%).Allprescriptionoforalantibiotics inadultpatients is theappropriate therapeuticdose.Thereare38prescriptionswithpotentialofantibioticdruginteractions,andthemost isdruginteractionsbetweenisoniazidandrifampicin (16 prescriptions). All antibiotic prescriptions (100%) are appropriate to the hospitalformulary, and there are 79 prescriptions (9.27%) of antibiotics that are not appropriate to thenationalformulary.Itcanbeconcludedthatthereissomepotentialfordruginteractionsandthereareantibioticprescriptionsthatarenotappropriatetotheformulary.Keywords:antibiotics,profile,Formulary


159


ISOLATIONANDANTIMICROBIALACTIVITYOFENDOPHYTICFUNGIFROMSHIITAKEMUSHROOM

(Lentinulaedodes)

IkaKurniaSukmawati*,AriYuniarto,MuhamadFaizalRofik

BandungSchoolofFarmacy,SukarnoHattastreetNo754CibiruBandung,40614*E-mail:[email protected]

ABSTRACT

Endophytesproducesecondarymetabolitecompoundshasgreatpotentialforthedevelopmentofnewdrugs.Endophytescouldbeproducedfromvariousplantsoneofwhichisshiitakemushrooms(Lentinula edodes). The aims of this study to isolate and assay endophytic activity of shiitakemushroomsagainstbacteriaandfungi.Themethodwereusedinthisexperimentalincludespaperdiscmethodandmicrodilutionbroth.TheresultofendophyticbacteriaisolationwasobtainedbythreeisolatebacteriasuspectedofisolateS1(Bacilluscereus),isolateS2(Staphylococcussp),isolateS3 (Bacillus macerans) and isolate of endophytic fungi obtained 1 isolate suspected isolate F1(Penicillium). The results of antibacterial activity assay showed that the largest inhibitory zonediameteragainstEscherichiacoliwasfoundinisolateS1(30000ppm)withdiameterof10,14±0,63mmandtoStaphylococcusaureusinisolateS3(11000ppm)withdiameter9,19±0,68mm.Theresults of antifungal activity assay showed that the largest inhibitory zone diameter of Candidaalbicanswasfoundinconcentrationof34000ppmwithdiameter6.45±0.35mm.MICandMBCvaluesofantibacterialassaywasfoundinisolateS1concentrationsmorethan32000ppm,isolateS2morethan4000ppmandisolateS3morethan14000ppm.MICandMFCvaluesofantifungalassaywas found in concentrationof2125ppm.Theconclusion resultofendophyticassayasanantimicrobialactivityexpressedquitewell.Keywords:Shiitake,Antimicrobial,Isolation

PosterPresentation


160

[PP-61]

ANTIBACTERIALACTIVITYOFTESPONG(OenanthejavanicaDC),SINTRONG(Crassocephalumcrepidioides),ANDPOHPOHAN(PileatrinerviaW)AGAINSTBACTERIA

StaphylococcusepidermidisandPseudomonasaeruginosa

AsepRoni*,NataliaAnggraeni,WempiBudiana

BandungSchoolofPharmacyJL.Soekarno-HattaNo.754,Cibiru–Bandung,Indonesia


ABSTRACT

Tespong (Oenanthe javanica), sintrong (Crassocephalum crepidioides), and pohpohan (Pileatrinervia) is a type of indigenous vegetableswhich have antibacterial activity and consumed byIndonesianpeople,especiallypeopleofWestJava.Thestudywasconductedtodeterminethemostpotentantibacterialactivityamongleaveextractsoftespong,sintrong,andpohpohanandalsotodetermine the active compound that responsible for inhibiting Staphylococcus epidermidis andPseudomonasaeruginosabacteria.Theextractionwasdonebymacerationmethodusingethanol96% solvent. Antibacterial activity test was conducted by microdilution method and usingtetracycline as a comparison. The measured parameters were the Minimum InhibitoryConcentration(MIC).ThehighestantibacterialactivitywaspohpohanleafextractwithMICvaluesof 640 mg/mL against Staphylococcus epidermidis and 1280 mg/mL against Pseudomonasaeruginosa,aswellasn-hexanefractionofpohpohanwithMIC1280ug/mLinhibitStaphylococcusepidermidis and MIC values of 2560 mg/mL inhibit Pseudomonas aeruginosa. The results of abioautographytestofn-hexanefractionshowedthecontentofflavonoidwhichwassuspectedastheactiveantibacterial.Keywords: Antibacterial, bioautography, Indigenous vegetables, microdilution method,Pseudomonasaeruginosa,Sthaphylococcusepidermidi.

PosterPresentation


161

[PP-62]

Alpha-GlucosidaseInhibitionActivityofExtractandFractionsKupa(Syzygiumpolycephalum(Miq.)Merr.&Perry)Leaves

DadangJuanda*,LiaMarliani,MariaYolandaAgnestaMiaDapa

BandungSchoolofPharmacy

Jl.SoekarnoHattaNo.754Cibiru-Bandung40614,Indonesia*E-mail:[email protected]

ABSTRACT

Diabetesmellitusisachronicdiseasecausedbyreducedproductionofinsulinbythepancreasorineffectiveinsulinproduction.Diabetesmellitustype2occursin87-91%ofcasesofdiabetesintheworld, one approach in its treatment by usingα-glucosidase enzyme inhibitors. Plants from theMyrtaceae family especially the Syzygium genus have been extensively studied to have α-glucosidaseinhibitoryactivity.Kupa(Syzygiumpolycephalum)isaspeciesthathasnotbeenstudiedandfrompreviousstudycortexhasbeenshowntohaveα-glucosidase inhibitoryactivity (1).Thisstudy aimed to determine α-glucosidase inhibition activity of Kupa Leaves. Extraction wasperformed by reflux, fractionation by liquid-liquid extraction (LLE), inhibition of α-glucosidaseactivitywasdone invitroby spectrophotometric.α-glucosidase inhibitionactivityofextractandfraction, used α-glucosidase enzyme concentration 0.6 U/mL and pNPG substrate 15 mM. TheIC50forethanolextract,n-hexane fraction,ethylacetate fraction,ethanol fraction,quercetinandacarbose tablet respectively 17.853; 202.838; 11.180; 13.957, 23.546 and 123,684 μg/mL. Ethylacetatefractionhavethestrongestinhibitionofα-glucosidaseandSyzygiumpolycephalumhasthepotentialtobedevelopedasanalternativesourceoftreatmentfordiabetes.Keywords:diabetesmellitus,α-glucosidase,Myrtaceae,kupa,Syzygiumpolycephalum

PosterPresentation


162

[PP-64]

STUDYOFCORNSTARCHORIGINATEDFROMLOCALCORNASBINDERANDDISINTEGRANTINTABLETS

MarlineAbdassah*,Sriwidodo,AnisYohanaChaerunisaa1)DeptofPharmaceuticsandPharmaceuticaltechnology

FacultyofPharmacy,UniversitasPadjadjaran*E-mail:[email protected]

ABSTRACTCorn is abundantly cultivated in Indonesia.With regard to its use as pharmaceutical excipient,Indonesia still highlydependenton imported starch thereforedevelopmentof starch from localoriginatedcornisinhighneed.Theaimofthisresearchwastostudythecharacteristicoftabletswhen local corn (ZeamaysL.) is usedasbinderordisintegrant. Tabletswere formulatedbywetgranulationmethodusingvariousconcentrationofbinderandcomparedwiththoseusingstandardcornstarch(Roquette).Tostudytheeffectoflocalcornstarchasdisintegrant,theophyllinetabletwere formulated, evaluated and compared with those using marketed product disintegrant(Roquette,Acdisol,Na-CMC).Theresultsofevaluationonthegranulesandtabletsshowedthattabletsusing local cornstarchasbinderhadnearly thesamecharacteristic comparedwith thoseusingstandardcornstarch.Whenlocalcornstarchwasusedasdisintegrant,evaluationongranulesand tablets of theophylline showed that thosewhichmade bywet granulation revealed betterphysicaltabletqualitythanthatmadebydirectcompression.Tabletformulationwith5%ofcornstarchrevealedhardnessasmuchas75.33N,friability0.0876%anddisintegratingtimeof1minute52seconds.Theinfluenceoflocalcornstarchondrugreleasewereinvestigatedbyemployingmodeldrugwithdifferentsolubilityintabletformulations.Theresultsshowedthattherewasnoinfluenceoflocalcornstarcheitherasabindernorasdisintegrantondrugrelease,forthestronginfluencewasduetothesolubilityofactivesubstances.Itcanbeconcludedthatlocalcornstarchprovidednearlysamecharacteristicswithstandardmarketedone(Roquette)eitherwhenusedasbinderordisintegrant.Keywords:Cornstarch,tablets,binder,disintegrantPosterPresentation


163

[PP-66]

SKINPENETRATIONABILITYOFPANTHENOLGELWITHGHKCUCOPPERPEPTIDECOMPLEXES

YanniD.Mardhiani1,*,DadihSupriadi1,TeguhYanuarSantosa1,TaofikRusdiana2

1SekolahTinggiFarmasiBandung,Jl.SoekarnoHattaNo.754,Bandungcity,westJava,Indonesia.2DepartmentofPharmaceuticsandPharmaceuticalTechnology,FacultyofPharmacy,Universitas

Padjadjaran,Jatinangor,Sumedang,WestJava,Indonesia*E-mail:[email protected]

ABSTRACT

Provitamin B5 (panthenol) has been reported to decrease many signs of skin aging, includingwrinkle,hyperpigmentationandredness.Incosmeticformulations,itprovidesasynergisticeffectas an anti-wrinklewhen combinedwith copper peptide. In this study, other benefits of copperpeptide had been investigated as a penetration enhancer. A gel dosage form was formulatedcontaining panthenol with and without GHK-Cu copper peptide (tripeptide glycyl-L-histidyl-L-lysine)complexes.SkinpenetrationabilityofprovitaminB5wasmeasured invitrowiththemodelsystem,amodifiedFranzdiffusioncell,usingratabdominalmembraneasastandardmodelofaskinbarrier.Physicalevaluationsofthegelof3%panthenoland4%copperpeptideasthebestformulawereexaminedandobtainedthesedata:pH=5.62,viscosity=1428cps.Alloftheformulawerestable at rheological stability testswhich carried out at the room temperature and freeze thawcycling. The in vitro penetration testing showed significant differences in all formulas at asignificancelevelof0.05.Basedontheresultsofthisstudy,skinpenetrationabilityofpanthenolinthegelinceased[5]withtheexistenceofGHKCucopperpeptidecomplexes.Keywords:GHKCucopperpeptidecomplexes,provitaminB5,panthenol,gel,penetrationenhancer

PosterPresentation


164

[PP-67]

MOLECULARDOCKINGANDADMETPREDICTIONOFQUERCETINANALOGSINDISCOVERYOFuPAINHIBITOR

BinaLohitaSari1,2*,SlametIbrahimSurantaatmadja1,DaryonoHadiTjahjono1

1SchoolofPharmacy,BandungInstituteofTechnology,JalanGanesha10,Bandung40132,Indonesia

2StudyProgramofPharmacy,PakuanUniversity,JalanPakuanPOBox452,Bogor16143,Indonesia*E-mail:[email protected]

ABSTRACT

Inrecentyears,flavonoidshavegainedattentionduetotheirpotentanticanceractivity.Quercetinisaflavonolwithhydroxylandoxogroupspresentinitsstructure.Thepooradsorption,lowsolubilityinwater,highrateofmetabolism,highenzymaticdegradationandrapidclearancefromthebodyofquercetinexerts its toxicityonnormalhumancell lines. Therefore, there is aneed tomodifyquercetinstructuretoobtainappropriatephysicochemicalproperties.Urokinase-typeplasminogenactivator(uPA)isamemberofserineproteaseenzymesthatassociatedwithcancerprogression.Inordertounderstandthirty-sixofquercetinanalogsbindtotheuPAenzyme,eachcompoundwastakenfordockingstudiesbyusingMOEwiththeenzyme(PDBID1C5X).ThecompoundswithbestbindingenergywerethentreatedtheirADMETanalysis.Moleculardockingstudiesrevealedthat4’-ethylquercetinwasmostpotentmoleculebound intotheactivesiteofuPA(bindingenergy -121.790 kcal/mol) and 3,7,4’-trimethyl quercetin showed the lowest carcinogenicity (TD50 in rat0.0024mg/kgBB). For further study, the two compounds need to be tested in vitro using uPAinhibitoryenzyme.Keywords:quercetinanalogs,uPAinhibitor,moleculardocking,ADMETprediction

PosterPresentation


165

[PP-68]PHYSICOCHEMICALCHARACTERISTICSANDQUANTITATIVEANALYSISOFS-ALLYLCYSTEINEOF

BLACKGARLIC

YoppiIskandar1,*,EllinFebrina2,YediHerdiana3

1DepartmentofBiologicalPharmacy,FacultyofPharmacy,UniversitasPadjadjaran,Jl.RayaBandung-SumedangKm.21Jatinagor45363WestJavaIndonesia

2DepartmentofPharmacologyandToxicology,FacultyofPharmacy,UniversitasPadjadjaran,Jl.RayaBandung-SumedangKm.21Jatinagor45363WestJavaIndonesia

3DepartmentofPharmaceuticsandPharmaceuticalTechnology,FacultyofPharmacy,UniversitasPadjadjaran,Jl.RayaBandung-SumedangKm.21Jatinagor45363WestJavaIndonesia


ABSTRACTGarlic (Allium sativumL.) is a species of the onion genus that has long been used as a culinaryseasoningandmedicalherb(1).Blackgarlic(BG)isanewlyprocessedfoodpreparedbysubjectingwhole rawgarlic to thermalprocessingat70∼80oCundercontrolledhumidityconditions for1∼3months without additives (2). This research aimed to investigated the physicochemicalcharacteristics and quantitative analysis of S-allyl cysteineof black garlic (BG). The test resultsobtained from black garlic extract were: compound contents dissolved in water was 5.6%,compoundcontentsdissolved inethanolwas7.6%, levelsofS-allylcysteinewas10.9%,totalashcontentwas2.8%,ashcontentinsolubleinacidwas0.6%.Keywords:blackgarlic,physicochemicalcharacteristics,S-allylcysteine

PosterPresentation


166

[PP-69]

THEANTIOBESITYEFFECTOFETHANOLEXTRACTANDVARIOUSFRACTIONSOFMALAYAPPLE[Syzygiummalaccense(L.)Merr&Perry]LEAVESONWISTARFEMALERATSINDUCEDBYHIGH

CARBOHYDRATEFOODANDMONOSODIUMGLUTAMATE

AtunQowiyyah1,*,ElinYulinahSukandar1,MuhamadInsanu11SchoolofPharmacy,InstitutTeknologiBandung,GaneshaStreet10Bandung,Indonesia,

40132*Email:[email protected]

ABSTARCT

Malayapple(Syzygiummalaccense(L.)Merr.&Perry)hashypolipidemiceffect1andthemethanolextractofS.malaccenseleavescouldinhibitupto99%ofpancreaticlipaseactivity2whichisoneofthemechanismofactionofantiobesitydrugssoitassumesthatmalayappleleaveshaspotencytobe antiobesity agent. The aim of this studywas to investigate the antiobesity effect of ethanolextractandvariousfractionsofmalayapple(S.malaccense(L.)Merr.&Perry)leavesonobeseWistarfemale rats. The ethanol extract were prepared bymacerationmethod using 96% ethanol andfractination process was carried out using liquid-liquid extraction method with n-hexane,ethylacetateandwaterassolvents.ThisstudywasconductedonWistarfemalerats inducedbyhighcarbohydratefoodfor45daysandsubcutaneousMSG(MonosodiumGlutamate)injectionatadoseof2g/kgbwfor5consecutivedaysintoobesity.Theethanolextractatadoseof50mg/kgbw;n-hexane,ethylacetate,andwaterfraction;andorlistatatadoseof32.4mg/kgbwascomparatordrug, all given for 14 days. Induction with high carbohydrate food was continue during thetreatment.Resultshowedthattheinductioncouldincreaseratbodyweightwiththerange197.7-365.1%.Theethanolextractofmalayappleleavesatadoseof50mg/kgBW,n-hexaneandethylacetatefractionhaveantiobesityeffectbyinhibitthebodyweightgainsignificantlycomparedtopositive control group (p<0.05). The highest effect showed by n-hexane fraction with 79.8%inhibitionofbodyweightgaintopositivecontrolgroup.Theextractandallfractionsdidn’taffectfecal,food,andliverindex;butcoulddecreasedtheabdominalfatindexwherethelowestindexshowedbyn-hexanefraction(0.87±0.38%).Theextractandallfractionsofmalayappleleavescouldaffectthe lipidprofile(triglyceride,totalcholesterol,HDL-cholesterol,andLDL-cholesterol levels)butstatisticallynotsignificant.Observationofliverhistopathologyshowedthattheextractandallfractionscouldn’trepairedliverfatdegeneration.Theethanolextract,n-hexaneandethylacetatefractionofmalayappleleaveshaveantiobesityeffect.Theextractandallfractionsdidn’tshowedlaxative and anorexic effect. The extract and all fractions could decrease lipid deposition onabdominalfattissuebutcouldn’trepairedliverfatdegeneration.Keywords:antiobesity,bodyweight,malayapple,Syzygiummalaccense,n-hexanefraction


167


EVALUATIONOFTHEPOTENCYOFENDOPHYTICFUNGIEXTRACTSASSOCIATEDWITH

POTENTIALLYMEDICINALPLANTSFROMMANDALIKA-LOMBOK,WESTNUSATENGGARA

Praptiwi*,AhmadFathoni,DewiWulansari,MuhammadIlyas,MarlinM.Raunsai,andAndriaAgusta

ResearchCenterforBiology,IndonesianInstituteofSciences

Jl.RayaJakarta-BogorKm.46Cibinong,16911.*Email:[email protected]

ABSTRACT

The secondary metabolites of endophytic fungi generally similar to the host plant and haveimportantbiologicalactivitiesThisstudyaimsweretoevaluatethepotentialof35endophyticfungiextractsassociatedwith12potentiallymedicinalplantscollectedfromMandalika,Lombok,WestNusaTenggaraasantibacterialandantioxidant.AntibacterialwascarriedoutbyTLC-bioautographymethod against S.aureusand E.coli. Antioxidant activity test was carried out by TLC DPPHbioautographybyDPPHmethod.TheactiveextractsweredeterminedbytheirMIC’sandIC50valuesby serial dilutionmethod in 96wellmicroplates. The results showed that therewere 23 activeextracts as the antioxidant were categorized as follows: 9 extracts were poor, 7 extracts aremoderate,4extractsarepowerful,3extractsareverypowerfulantioxidantactivity.Therewere32extractsinhibitedthegrowthofS.aureuswerecategorizedasfollows:19extractswereweak,12extracts were moderate inhibition. There were 18 extracts inhibited the growth of E.coliwerecategorizedasfollows:15extractswereweakand3extractshavemoderateantibacterialactivityagainstE.coli.TheconclusionofthisstudyisthatendophyticfungifrompotentiallymedicinalplantscollectedfromMandalikahavethepotentialasanewantibacterialandantioxidantsources.

Keywords:Mandalika,endophyticfungi,potentiallymedicinalplants,antibacterial,antioxidant


168


RADIOLABELINGOFPLANTARICINFASANATURALANTIBIOTICUSINGIODINE-131

EvaMariaWidyasari1*,IstiDaruwati1,MaulaEkaSriyani1,RizkyJuwitaSugiharti2and

AponZaenalMustopa2

1CenterforAppliedNuclearScienceandTechnology,NationalNuclearEnergyAgency(BATAN),

JalanTamansariNo71Bandung,Indonesia2ResearchCenterforBiotechnology,IndonesianInstituteofScience(LIPI),JalanRayaBogorKm46

Cibinong16911Bogor*Email:[email protected]

ABSTRACT

Plantaricin F is bacteriocins produced by Lactobacillus plantarum S34 that was isolated from“Bekasam”(Indonesiatraditional-fermentedmeat).PlantaricinFhaspotentialapplicationstofightmicrobial infectionsandcontaminationssomostlyappliedinagriculturalasfoodbiopreservationagent. This study is aimed to find the labeled condition of plantaricin F using iodine-131radioisotope.I-plantaricinFlabeledcompoundwillbeusedasradiotracertostudybiodistributionofplantaricinFininfectionanimalmodelinthefutureresearch.I-plantaricinFwithradiochemicalpurity95.27±0.69%waspreparedusing60µgplantaricinF,10µlNaI,150µgchloramine-T,300µgsodiummetabisulfiteinaTrisHClbuffersolution(pH7.5)andincubatedinroomtemperaturefor60seconds.TheradiochemicalpuritywasdeterminedbyusingWhatmann1chromatographypaperwithmethanol 90% asmobile phase.With this success, I-plantaricin F labeled compoundmaypotentiallyuseasaradiotracerforin-vivostudyinthenextresearch,thereforetheeffectivenessofplantaricinFasanaturalantibioticcouldbeproven.Keywords:I-plantaricinF,labeledcompound,radiotracer,antibiotic


169


BIODISTRIBUTIONOF99mTc-HSA-NANOPARTICLEINLYMPHATICNODEOFANIMALMODEL

INDUCEDBY7,12DIMETHYLBENZ(A)ANTHRACENE

RizkyJuwitaSugiharti*,IimHalimah,IsaMahendra,EvaMariaWidyasari,MaulaEkaSriyani,WitriNuraeni

CenterforAppliedNuclearScienceandTechnology–NationalNuclearEnergyAgency

Jl.Tamansari71-Bandung,40132*Email:[email protected]

ABSTRACT

Breastcancerisoneofthemostcommonmalignanttumorsfoundinwomenandhasbecomeasthesecondcauseofdeathinwomenaftercervicalcancer.Wesuccessfullysynthesizedtechnetium-99mlabeledHumanSerumAlbuminnanoparticle(99mTc-HSAnanoparticle);aradiopharmaceuticalwhich potentially used for application in lymphoscintigraphy technique for tracing lymphaticsystem. This study was aims to obtain pre-clinical data from 99mTc-HSA nanocolloid in SpragueDawleyfemale ratsgiven7,12-dimethylbenzeneanthracene (DMBA) - inducedcellproliferation inthebreasttissues.Theratsaredividedintofourgroups,threegroupsaregivenDMBAfor5,8and12weeksandtheothergroupiswithoutanytreatmentascontrol.Biodistributionstudyof99mTc-HSAnanoparticlewas conducted to identify the accumulationof this agent in thepopliteal andlumbarlymphnodesandtheothertissuecomparetonormalrats.99mTc-HSAnanoparticleshowngoodaccumulationinpopliteallymphnode1.47+1,23%ID;0.81+0.47and1.04+0.46%IDatone hour post injection in 5, 8 and 12 weeks respectively. The popliteal extraction (PE) wasestimatedwithvalue89.88%,73.78%,72.03%in5,8and12weeksrespectively.Thisresultshowedthat 99mTc-HSA nanoparticle is very promising compound to be lymphoscintigraphy agent indetectionofbreastcancer.Keywords:lymphoscintigraphy,99mTc-HSAnanoparticle,breastcancer


170


ANTIOXIDANTACTIVITYOFETHANOLICEXTRACTANDITSFRACTIONSOFPandanus

tectoriusLEAVESBYREDUCTIONINHIBITIONOFWATER-SOLUBLETETRAZOLIUMSALT(WST-1)METHOD

MoelyonoMoektiwardoyo*,AjengDiantini,andPriscillaTaniaWijaya

FacultyofPharmacy,UniversitasPadjadjaran,Jl.RayaBandung-SumedangKM21Jatinangor,

Sumedang45363*Email:[email protected]

ABSTRACT

ThemethanolextractofPandanusodoratissimusleaveshadbeen researchedcancatchdirect[1]free radical 1,1-Diphenyl-2-Picrylhydrazyl (DPPH)with an IC50is 36μg/mL.Antioxidant activity ofethanolicextractand its fraction ofanotherPandanusspecies, i.e.Pandanus tectoriusleaveshadbeenobservedbySODmethod.Thepurposeof this research is toknowtheSOD likeactivityofethanol extracts, n-hexane fraction, ethyl acetate fraction and water fraction of Pandanustectoriusleaves.Inaddition,theresearchalsoaimstodeterminethesecondarymetabolitesfoundinthefractionwhosubmittedthebestactivity.VitaminCisusedasapositivecontrol.TheresultsshowedthatthefractionofethylacetategivesSOD-likeactivitywithIC50=573,79μg/mL,ethanolicextract IC50=656,60μg/mL;waterfractionIC50=846,79μg/mL,andn-hexanefraction IC50=860,00μg/mL while vitamin C having IC50=72,37 μg/mL[2] . Secondary metabolites found in the ethylacetatefractionareflavonoids,steroids,monoterpenesandsesquiterpenoids.Flavonoidsareoneofthecompoundbasedonliteratureknownasantioxidantactivecompounds.Basedontheresultsof flavonoids investigation by using UV-Visible spectrophotometry, predicted on ethyl acetatefractioncontainstypeofflavonoids:flavones,flavonolorisoflavones.Keywords:Pandanustectorius,SODlikeactivity,flavonoids


171


PREPARATIONOFRUTINLABELEDSCANDIUM-46ANDOPTIMIZATIONOFITSTLCANALYSIS

MuhamadBasitFebrian*,BadraSandityaRattyananda,YakobusPrima,EvaMaria

Widyasari,RizkyJuwitaSugiharti,DuyehSetiawan

CenterforAppliedNuclearScienceandTechnology,NationalNuclearEnergyAgency,Jalan

Tamansari71Bandung40132*Email:[email protected]

ABSTRACT

Rutin (rutoside or vitamin P) and its complexes have been investigated for its pharmacologicaleffects. They have both anticancer effect and chemotherapeutic activity according to someresearches.Asaderivativeofquercetinflavonoidsgroup,rutinhasabilitytoformcomplexeswithsome metals including metal radioisotopes. Scandium-46 (46Sc) could be produced by neutronactivationmethodinresearchreactor.46Scradioisotopesemitsradiationandbetaradiationwith83daysofhalf-life.Thesecharacteristicsmade46ScsuitableforlabelingandbiodistributionstudyofSc-Rutin complex. This study aims to perform 46Sc-Rutin complex as natural product labeledradioisotopecompoundtogetherwithoptimizationofitsTLCanalysis.Rutinwasdirectlymixedandreactedwith46ScCl3afterdissolvedinmethanolorwater.Aseriesofvariationofmolarratiobetweenrutinand46Scsolutionwasinvestigatedtofindoptimumconditionof46Sc-Rutincomplexcompound.Formed 46Sc-Rutincomplexandunreacted 46Scwas separatedbyvariousTLC system inorder todetermineoptimumTLCsystemin46Sc-Rutinanalysis.Inthisstudy,TLCsystemwithmethanoland10%ammoniumacetate(w/w)asmobilephaseand10cmwhatmann31ETchromatographicpaperasstationaryphasewasfoundasoptimumTLCsystemfor46Sc-Rutinanalysis.Retardationfactor(Rf)of46Sc-Rutinandunreacted46Scwere0–0.125and0.5–0.625respectively.VariationsofmolarratioSc:rutinat5:1,4:1,3:1,2:1,1:1,1:2and1:3resultedinoptimummolarratioat1:1with52.2%labelingyield.Insummary,rutincouldreactdirectlywith46Scradioisotopeandresultedin46Sc-Rutinlabeledcompound.Furtherexperimentwillbecarriedouttoimprovelabelingyieldandtoassessothercharacteristicsof46Sc-Rutinlabeledcompoundincludingphysico-chemical,pharmacologicalandbiologicalexamination.Keywords:Rutinlabeledcompound,directlabeling,Rutin,46Sc-Rutin,TLCAnalysis


172


XANTHINEOXIDASEINHIBITIONACTIVITYOFGADUNGTUBER(DIOSCOREAHISPIDA),COMMONPLANTAINLEAVES(PLANTAGOMAJORL.),COMFREYROOTS(SYMPHYTUM

OFFICINALEL.),ASTHMAWEEDLEAVES(EUPHORBIAHIRTAL.)\

AmiTjitraresmi*,MoelyonoMoektiwardoyo,EvarianiDwiWulandari

FacultyofPharmacy,UniversitasPadjadjaran,Jl.RayaBandungSumedangKM.21,Hegarmanah,Jatinangor,KabupatenSumedang,JawaBarat45363

*Email:[email protected]

ABSTRACT

Uricacidisanitrogenouscompoundthatisthefinalbreakdownproductofpurine(aDNAbuildingblock) catabolism. The enzyme xanthine oxidase catalyses the oxidation of hypoxanthine andxanthinetouricacid.Excessiveproductionofuricacidinthebodycancausehyperuricemiaandhighlevelsofblooduricacidhavebeenassociatedwithgout(1).Thisstudyaimstodeterminexanthineoxidase inhibition activity of gadung tuber (Dioscorea hispidaDeenst.), commonplantain leaves(Plantago majorL.), comfrey roots (Symphytum officinaleL.), asthma weed leaves (EuphorbiahirtaL.).TheseherbshasbeenusedinGorontalotribe,Indonesia,astraditionalmedicinefortreatinghyperuricemia(2).Eachherbwasmaceratedwithethanol70%.XanthineoxidaseinhibitionactivityofplantextractswascarriedoutbyinvitroassaytodetermineIC50values.Theresultsshowedthatgadung tuber (Dioscorea hispida), common plantain leaves (Plantago majorL.), comfrey roots(SymphytumofficinaleL.),asthmaweedleaves(EuphorbiahirtaL.)extractsatadose50μg/mlcouldinhibittheactionofxanthineoxidasewithinhibitoryvalues53.49%;62.53%;53.49%;and61.28%,andtheIC50oftheseplantextractwere47.14μg/mL;38.57μg/mL;42.93μg/mL;And31.57μg/mLrespectively.Asthmaweedleavesextracthadthehighestxanthineoxidaseinhibitionactivity.Keywords:DioscoreahispidaDeenst.,PlantagomajorL.,SymphytumofficinaleL.,EuphorbiahirtaL.,hyperuricaemia,uricacid,xanthineoxidaseinhibitor


173


TheEffectofCeraalbaConcentrationVariationsonThePhysicalStabilityofBlackCumin(Nigella

sativaL.)OilBalmStickKoriYati*,LusiPutriDwita,SriNeviGantini,Nurusysyifa,SeptianaTriPamungkas

ProgramStudiFarmasi,FakultasFarmasidanSains,UniversitasMuhammadiyahProf.DR.HAMKA,

JakartaTimur,Indonesia*Email:[email protected]

ABSTRACT

Black cuminoil fromblack cumin seeds contained thymoquinone (TQ) and known to have anti-inflammatoryactivity. Topical anti-inflammatorydrugs canbemade in the formofbalm. In thisstudy,blackcuminseedoilwasformulatedintoastickbalminordertofacilitateitsuse.Methodinthis study,black cumin oil was formulated in the form of a balm stick using cera alba, adepslanae,andVCOasabase.Balmstickwasmadeinto5formulaswithvaryingconcentrationsofceraalba(30%, 32.5%, 35%, 37.5% and 40%). The evaluation of the balm stick was carried out onorganoleptic,homogeneity,meltingpoint,hardnessandyieldvalue,followedby8weeksphysicalstability evaluation at room temperature. The results obtained were analyzed using Two WayANOVAandKruskalWallisstatisticalanalysis.Physicalstabilitytestresultsindicatedanincreaseinmeltingpointinthebalmstickonaweeklybasis(50.17oC,51.33oC,52.83oC,55.33oCand57.17oCrespectively), while the results of the hardness test and yield value in stick balm were102.89dyne/cm2,113.54dyne/cm2,125.93dyne/cm2,137.79dyne/cm2and148.68dyne/cm2respectively,whichwerealsoshowedanincreaseinvalue.Inspiteofincreasedonmeltingpoint,hardnesstestandyieldvalueonphysicalstabilitytests,thisresultstillmettherequirements.Basedonthe8weeks physical stability evaluation at room temperature, it can be concluded thatincreasedconcentrationofceraalbainthebalmstickformulationaffectedthemeltingpoint,hardnesstestand yield value of black cumin oil balm stick, however, the results were still in the range ofrequirements.Keywords:Blackcuminoil,ceraalba,balmstick,physicalstability.


174


ANTIOXIDANTACTIVITYOFTHREEKINDSOFRICEBRAN(RED,BLACKANDWHITE)WITHDPPHASSAY

ZelikaMegaRamadhania1,2*,AmiTjitraresmi1,2*,RiniHendriani1,2,RadenBayuIndradi1,2,Mia

Nurlinda1

1FacultyofPharmacy,UniversitasPadjadjaran,RayaBandungSumedangKm.21,

Jatinangor45363,WestJava,Indonesia2ScientificConsortiumofHerbal,UniversitasPadjadjaran,RayaBandungSumedangKm.

21,Jatinangor45363,WestJava,Indonesia*Email:[email protected]

ABSTRACT

Ricebranisaby-productofricemillingobtainedfromtheouter layerofricekaryopsis.Previousresearchhasshownthatricebranpotentialasnaturalantioxidants.Theresultsshowedthatthericebran contains high bioactive components such as tocopherols, tocotrienol, oryzanol, phenolicantioxidants,β-caroteneandanthocyanins.Therearethreekindsofricebran:red,black,andwhitericebran.Thisstudywasconductedtodeterminetheantioxidantactivityofred,black,andwhitericebranextractthatderivedfromWestJava.Theantioxidantactivityofallthreetypesofricebranextractswasdeterminedbyusing2,2-diphenyl-1-picrylhydrazyl(DPPH)andthenmeasuredbyusingUV-visible spectrophotometer. Extraction was done by maceration method. The solvent usedethanol95%thatisacidifiedbyHCl37%tillpH1.Theresultsshowedthatallthreetypesofricebranextractshasantioxidantactivity.BlackricebranextractshadthelowestIC50value,followedbyredandwhitericebranextractwithIC50values inarowat218.5813µg/mL,855.5685µg/mL,and1129.136µg/mL.Keywords:Antioxidant,RiceBran,DPPH


175


ANTIBACTERIALACTIVITYOFEleutherinepalmifoliaL.Merr.

ETHANOLICEXTRACTAGAINSTBacilluscereus

ImamAdiWicaksono1*,ImanFirmansyah2,RadenBayuIndradi2

SulistiyaningsihSulistiyaningsih2

1DepartmentofPharmacologyandClinicalPharmacy,FacultyofPharmacy,UniversitasPadjadjaran,Jatinangor–Indonesia,45363.

2DepartmentofBiologicalPharmacy,FacultyofPharmacy,UniversitasPadjadjaran,Jatinangor–Indonesia,45363.


ABSTRACTBacillus cereus can cause a wide range of diseases in humans, including foodborne illness andsystemic infection.1B. cereus-induced gastroenteritis, bloodydiarrhea andemetic poisoning.2ThediarrheasyndromeiscausedbyenterotoxinsproducedbyB.cereus,theemeticsyndromeiscausedbycereulideduringthegrowthphaseinthefood.2,3Intraditionalmedicine,EleutherinepalmifoliaL.Merr. (Dayak Onions) are commonly used as antibacterial to various infectious diseases. Thisresearchwasalreadyconductedtodeterminetheantibacterialactivities,determinethevalueofminimum inhibitory concentration (MIC) andminimum bactericidal concentration (MBC) of theextractagainstBacilluscereus.TestforantibacterialactivitycarriedoutbyagardiffusionmethodandMICandMBCvaluedeterminedbydilutionmethod.TheresultshowedthatethanolicextractofdayakonionshasantibacterialactivityagainstBacilluscereusbacteria.ThisextracthasMICandMBCvalueoftheextractintheconcentrationrangeof1.25%(w/v)–2.5%(w/v).Theantibacterialactivityofthisextractprobablyderivedfromflavonoidsandpolyphenols.Keywords:Antibacterial,Bacilluscereus,EleutherinepalmifoliaL.Merr.,Foodborneillness


176


OPTIMATIONOFGENEENCODINGANTIHER2scFv[pD861-pelB]OVEREXPRESSIONINEscherichiacoliBL21(DE3)

TinaRostinawati*,SriAgungFitriKusuma,ImamAdiWicaksono,KelvinAldrinLusikooy1),MuhammadYusuf,TotoSubroto2)

1)FacultyofPharmacy,PadjadjaranUniversity,JlRayaBandungSumedangkm21Jatinangor,

Sumedang,45363,WestJava,Indonesia2)FacultyofMathematicsandNaturalSciences,PadjadjaranUniversity,JlRayaBandungSumedang

km21Jatinangor,Sumedang,45363,WestJava,Indonesia*Email:[email protected]

ABSTRACT

Breast Cancer is a malignant tumor that attacks breast tissue derived from glands, glandularchannels, andbreast support tissue.OverexpressionofHER-2 (HumanEpidermalGrowth FactorReceptor2)isoneofthereasonsofbreastcanceroccurrence.Anti-HER2scFvrecombinantproteinis an alternative for breast cancer detection other than the monoclonal antibody. Anti-HER2recombinantproteinwasproduced inEscherichia coliBL21(DE3) throughoverexpressionof geneencodingantiHER2scFvwithin[pD861-pelB]plasmid.So,antiHER2scFvcanbedeliveredtotheextracellular medium. To produce anti-HER2 scFv in optimum condition, optimization of geneencodingantiHER2scFvoverexpressionwasdeterminedwith1x,1.5xand2xmediastrengthandl-rhamnoseconcentrationsof40µM,1mM,2mM,4mM,dan6mM.Anti-HER2scFvoptimallywasoverexpressed using 1x liquid growthmedium (Luria Bertani broth) andwith 4mM l-rhamnoseconcentration after 4 hours of induction observed in the periplasmic fraction. After optimumconditionobtained, re-expressionwasperformedusingoptimumcondition.Extracellularproteinobtained was then purified using nickel polyhistidine tag affinity chromatography (Ni-NTA).However,purificationofanti-HER2scFvhadnotbeensuccessfulusingthismethod.Keywords:Overexpression,optimalcondition,mediastrength,l-rhamnoseconcentration


177

PosterPresentation [PP-80]MOLECULARDOCKINGSTUDIESANDVIRTUALSCREENINGOFCOMPOUNDSFROMINDONESIANNATURALPRODUCTSASPOTENTIALNEURAMINIDASEH5N1INHIBITORSRinaFajriNuwarda1,3*,ZelikaMegaRamadhania1,ImamAdiWicaksono1,3,MuhammadYusuf2,3,

MuchtaridiMuchtaridi1

1FacultyofPharmacy,UniversitasPadjadjaran,Jl.RayaBandungSumedangKM.21,.Jatinangor45363Indonesia.

2DepartmentofChemistry,FacultyofMathematicsandNaturalSciences,UniversitasPadjadjaran,Jl.RayaBandungSumedangKM.21,Jatinangor45363Indonesia.

3ResearchCentreofMolecularBiotechnologyandBioinformatics,UniversitasPadjadjaran,JalanSingaperbangsaNo.2Bandung40132Indonesia.


ABSTARCT

Indonesiahasbecomeoneof theworld’shighest case fatality ratesofH5N1humancases,withnumberofdeaths167fromtotal199cases.Theemergenceofviralresistancetowardsthecurrentanti-influenzadrugsneuraminidase(NA)inhibitorsrequiredthediscoveryofnewinhibitors.Inthecurrentdevelopmentofdrugs,naturalproductsareconsideredasoneoftheimportantsourcesofmedicinalagentsincludinganti-influenzaagents.Medicinalplantswhichcontainflavonoids,(oligo)stilbenes,coumarins, and diarylheptanoids were believed to have potential activity asneuraminidase inhibitors.Duetothe fact that Indonesiahasanabundanceofnatural resources,thus, the aim of this study were to select the Indonesian plants contain previous mentionedcompoundsand investigatetheir insilicoactivitiesusingmoleculardockingandvirtualscreeningmethodsagainstH5N1neuraminidase.Inthisstudy,docking-basedvirtualscreeningofcompoundsfrom 19 plants to quickly select in silicohits to be potential NA inhibitors was performed.Subsequently,theintermolecularinteractionsoftheinhibitorcompoundswiththereceptorH5N1Neuraminidase(PDBID2HU4)wereanalyzedto investigatethemostpreferred interactions.TheresultsshowedthatninecompoundsnamelybruceinsG,BruceosideC,cafestol,CepharadioneA,L-Theanine, neoandrographolide, ursolicacid and andrographolide were found having betterinteractions with H5N1 Neuraminidase receptors and therefore can be proposed for further invitroassayaspotentialneuraminidaseinhibitors.Keywords:Moleculardocking,MUNANAassay,neuraminidaseinhibitor,virtualscreening.


178


INVITROANDINSILICOEVALUATIONOFGENISTEINCONTAINEDINNUTSASAPROSTATICCANCER

GROWTHINHIBITOR

RiniHendriani1*,Nursamsiar2,AmiTjitraresmi3,DonaldEmilio1

1DepatrmentofPharmacologyandClinicalPharmacy,FacultyofPharmacy,UniversitasPadjadjaran,

Indonesia.2DepartmentofAnalyticalPharmacyandMedicinalChemistry,SekolahTinggiIlmuFarmasiMakassar,

Indonesia.3DepartmentBiologicalPharmacy,FacultyofPharmacy,UniversitasPadjadjaran,


ABSTRACT

The trend of back to nature using natural ingredients is increased in worldwide, so that naturalmedicine isan interestingtreatmentoptiontobedeveloped.Theaimofthepresentstudywastoexaminethephytoestrogeneffectscontainedinnutsontheprostatecancertherapy.CytotoxicitytestwasconductedinvitrobymeasuringcellviabilityusingMTTmethodandinsilicobydeterminingtheinteractionofphytoestrogen(genistein)andtamoxifen(ascomparisondrug)withestrogenreceptorbetaintermsofhydrogenbondsandbindingfreeenergy.DockingsimulationswereperformedbyAutoDock4.2package.Theresultinsilicostudyshowedthatgenisteinhadmorenegativefreeenergybinding-10,40kcal/molthanthatoftamoxifen-8,65kcal/mol.Furthermoreinvitrostudyshowedthatredbean(Phaseolusvulgaris)ethanolextracthadprostatecancercellgrowthinhibitionactivityhighenoughwith IC50177,24 μg/mL and ethanol extract of green bean (Vigna radiata)with IC50197,72μg/mLslightbiggerthangenisteinstandardwithIC5053,05μg/mL.Inconclusion,insilicostudy,itcanbepredictedthatgenisteinwasmorepotentialtoinhibitprostatecancergrowthbecausegenisteinhasahigheraffinitythanthatoftamoxifen,andinvitrostudytheredbeanethanolextractandthegreenbeanhadsmall IC50.Thustheextractsoftheredbeanandgreenbeancontainingtheactivecompoundgenisteinwerepotentialuseasaprostaticcancergrowthinhibitor.

Keywords:phytoestrogen,genistein,redbean,greenbean,prostatecancer,inhibitory.


179


Glibenclamide-AscorbicacidCocrystalSynthesizedByUsingtheSolventEvaporationMethod

toIncreasetheSolubilityofGlibenclamide

ArifBudiman1*,SandraMegantara2,PutriSaraswati1DepartmentofPharmaceuticandPharmaceuticalTechnology,FacultyofPharmacy,

UniversitasPadjadjaran,2DepartmentofPharmaceuticalAnalysisandMedicinalChemistry,

FacultyofPharmacy,UniversitasPadjadjaran,*Email:[email protected]

ABSTRACT

Solubility,dissolution,andgastrointestinalpermeabilityareimportantparametersthataffecttherateofdrugabsorptionanditsbioavailability.Thesolubilityofadruginwaterplaysanimportantrole in the absorption of the drug after oral administration.Glibenclamide is antidiabeticwhichbasedontheBiopharmaceuticalClassificationSystem(BCS),haslowsolubilityandhighpermeabilityresulting low bioavailability. Cocrystal is one method that improves the solubility of the activepharmaceuticalingredient(API)thatcontaincrystallinematerialpresentinadefinitestoichiometricamount.Theaimofthisstudyweretoinvestigatetheformationofaglibenclamide(GCM)-AscorbicAcid (AA) cocrystal using the solvent evaporation method and to evaluate its solubility anddissolutionrate.MoleculardockingoftheGCM-AAcocrystalwasobservedusinginsilicomethod.TheGCM-AAcocrystalwaspreparedbyusingthesolventevaporationmethod.Thecocrystalwasevaluated by the saturated solubility and the dissolution rate test (USP type 2 apparatus). Theproduct of GCM-APM was characterized by Fourier transform infrared spectroscopy (FT-IR),differentialscanningcalorimetry(DSC),andpowderX-raydiffraction(PXRD).TheresultofInsilicostudy revealed that the interactionofGCM-AAhashydrogenbondingwhich showedpotency inimproving the solubilityofGCM.EvaluationofGCM-AAcocrystal showed that the solubilityanddissolution rate were significantly increased. Characterization of the GCM-AA cocrystal(1:2)includingFT-IR,DSC,andPXRDshowedtheformationofanewsolidcrystalphasethatisdifferentfromGCMandAA.ThesolventevaporationproductofGCM-AA(1:2)providedtheformationofaGCM-AAcocrystalwhichhashighersolubilitythanGCM.

Keywords:Cocrystal,Glibenclamide,AscorbicAcid,SolventEvaporation


180


EFFECTIVENESOFAVOCADOSEEDS(PerseaamericanaMill.)EXTRACTELIXIRFORKIDNEYSTONES

SeptiaAndini*,LusiIndriani,ErniRustiani,danMoerfiah

DepartementofPharmacy,PakuanUniversityBogor*Email:[email protected]

ABSTRACT

Kidneystonesorcalculusarestonescontainedintheurinarytract,thestonesarecommonlyintheformsofcalciumcrystals.Avocadoseedisknowntocontainsseveralsecondarymetabolites,suchas alkaloids, flavonoids, tannins and saponins.Hydroxyl group (OH) of flavonoid compoundswillreactswithcalciuminthekidneysandformingcomplexcompoundissolubleinwater,sothatthewatercontainedintheurinewillhelpdissolvesthecalcium.Thisstudywastodeterminethelevelsofpotassiumintheavocadoseedextractelixirandtodeterminethesolubilityofcalciuminkidneystone invitro.Theresearchwasdonebyseveralmethodsas follows,producedofavocadoseedsextractusingboilingmethod,followedbyproducedofavocadoseedextractelixirwith70%ethanolandpropyleneglycol as a solventby comparison (5:10), elixir preparationmade then tested foreffectivenessaskidneystonesinvitrousingAAS.Thetestresultsofshowedthattheavocadoseedextractelixirwasbrownincolor,tastequitesweet,pHof5,315withaviscosityof19,64cpandthelevel of flavonoidswhich amount 2,102%, potassiumwhich amount at 0,050%. The test resultsshowed that the elixir could dissolved calcium kidney stones at level of 0.026%. So it can beconcludedthattheavocadoseedextractelixircontaincalciumandhasthepotentialtoshedkidneystonesatlevelof54,19%ofpositivecontrol.Keywords:AvocadoSeeds,Elixir,Potassium,KidneyStones.


181


SYNTHESISANDCHARACTERIZATIONOFIODINATEDRUTINTHROUGHOXIDATIONMETHODUSINGCHLORAMINE-T

MaulaEkaSriyani*,EvaMariaWidyasari,AjengRaffiNabila

NationalNuclearEnergyAgencyTamansariNo.71Bandung40132


ABSTRACTRutin(quercetin-3-O-rutinoside)isaflavonoidthatiswidelydistributedinplants.Rutinisfoundinmany tea plants and fruits such as oranges, grapes, lemons, limes,mulberries, and cranberries.SeveralstudieshavebeencarriedoutshowingthatRutinhastheabilitytoinhibitperoxidationoflow-densitylipoproteinandcanbeusedincancertherapy[1,2].Oneofthemethodsusedtodetectand treat cancer simultaneously is the labeledcompounds (radiopharmaceutical) that containedradioisotopeswhichtransmitgammaandalsobetaradiation,suchasIodine.Inthisstudy,IodineusedtoreactwithRutinandformiodorutinthroughadirectoxidationmethodatalkalinepHusingchloramine-Tasanaxidizingagent[3,4].Theaimofthisstudyistosynthesizedandcharacterizedtheiodinationproductbothphysicallyandchemically.TheproductwasthenanalyzedusingaUV-visiblespectrophotometer,FT-IR,and 1H-NMR.The resultsobtained that iodorutinwasdifferentfromitsinitialstate(Rutin).Thecrystalcolorwasbrownishyellowwiththemeltingpointat198-200°C.Theproducthasacubicshape,hygroscopicandsolubleinHCl,NaOH,andwater.Anewpeakappearedatawavelengthof224nmontheUV-VisibleSpectra,anabsorptionatwavelength±500nmusingFT-IRspectraandtherewasthelossof3chemicalshiftat6.20;6.39and6.89at1H-NMRspectra.Itcanbeconcludedthataproductofsodium-5',6,8-triiodorutinhassuccessfullysynthesizedusingdirectoxidationmethodwithchloramine-Tasanoxidizingagent.Keywords:iodination,Rutin,labeledcompound,synthesis,naturalcompound


182


DETECTIONOFFISHALLERGENSPREVALBUMINUSINGGELFILTRATIONCHROMATOGRAPHYG-

50ANDSDS-PAGE

EmmaEmawati1*,Idar1,DewiKurnia1,ShintiaAmelia1

SekolahTinggiFarmasiBandung,JalanSoekarnoHattaNo.754,CipadungKidul,Panyileukan,KotaBandung,JawaBarat40614,Indonesia*Email:[email protected]

ABSTRACT

Allergicreactions(hypersensitivityreaction)isthereactionsoftheimmunesystemthatoccurswhennormalbodytissueinjuryorhurt.Foodingredientsknowntocontainallergenproteinssuchasfish,milk,soybeans,peanuts,shellfish,eggs,wheat,andshrimpareoneofthemintunafish,theallergenproteinfoundintunaisPrevalbumin(10-13kDa).Thisstudyaimstodetectiontheallergenproteinsfound in tuna.Themethodused in this research isextraction,proteinseparationusingG-50gelfiltration column chromatography The principle of chromatography gel filtration is a separationbased on differences inmolecular weight, protein characterization using SDS-PAGE Principle ofelectrophoresis, ifachargedsubstancephase isgivenapotentialdifferencethephasewillmovealong a medium that is continuous towards the cathode or anode according to the particlecharge.Based on the results of research conducted on the extraction process, supernatant wasobtained,thentheproteinseparationprocessusingcolumnchromatographyobtained50fractionsand thecharacterizationprocessusingSDS-PAGEshowedthat therewasaprevalbuminallergenproteininaccordancewiththeliterature,namely10-12kDa.Keywords:chromatographygelfiltration,prevalbuminprotein,tunafish,andSDS-PAGE.


183


STUDYOFANTI-AGINGEFFECTIVENESSANDIRRITATIONOFDAYCREAMCONTAINING

TETRAHYDROCURCUMIN

IkaYuniAstuti*,DiahArdiana,AgusSiswanto,WahyuUtaminingrum,ArifBudiman

FacultyofPharmacy,UniversitasMuhammadiyahPurwokerto,DukuhwaluhStreet,Purwokerto,Indonesia53182


ABSTRACT

Sun and artificial UV rays can damage the skin and cause premature aging of the skin.Tetrahidrokurkumin(THC)isananti-agingagentthroughitsantioxidant,anti-inflammatoryandskinlightening effect. The aim of this study was to determine the anti-aging effectiveness and theirritationeffectofadaycreamcontainingtetrahydrocurcumin.Theanti-agingactivitytestusedapre-testpost-testcontrolgroupdesigninmice(MusmusculusL.).Theshaveddorsalskinofthemicewasappliedwiththetestedcreams,i.ethedaycreambaseasthenegativecontrol(NC),thecreamofethylascorbyletherasthepositivecontrol(PC),andthecreamcontainingtetrahydrocurcumin.At2hoursbeforeand15minutesafterUVirradiation,thesensitivity,collagencontent,elasticity,andwatercontentoftheskinwasmeasuredbyaskinanalyzer.Thisprocesswasrepeateddailyin8weeks.Theskinconditionatthebeginningandattheendofthetreatmentwascompared.TheirritationtestusedaHET-CAMmethodat5groups,i.eNaCl0.9%,NaOH0.1N,NC,PC,andTHC.Thehemorrhagetime,lysistime,andcoagulationtimeofthehen’seggwascalculatedusingirritationscore(IS)togetirritationindexvalue.ThegroupwiththecreamcontainingTHCtreatmentshowedasignificantdifferenceinsensitivity,collagencontent,elasticity,andwatercontentbeforeandafterthetreatment(p≤0.05).Basedonthescoresofalltheanti-agingparameters,theTHCcreamhadthesameanti-agingeffectivenessasPCandmoreeffectivethanNC.ThecreamcontainingTHCwaseffectiveasananti-agingandwasnotirritate.

Keywords:anti-aging,tetrahydrocurcumin,skinanalyzer,HET-CAM


184


MICROWAVE-ASSISTEDEXTRACTIONOFFLAVONOIDCOMPOUNDSFROMONIONSKIN(ALLIUM

CEPAL.)ANDANTIBACTERIALACTIVITYAGAINSTSTAPHYLOCOCCUSAUREUS

Sofihidayati*,FitriaDewiSulistiyono,andBinaLohitasari

StudyProgramofPharmacy,FacultyofNaturalSciences,UniversitasPakuan*Email:[email protected]

ABSTRACT

Onion (Allium cepaL.) are classified as vegetable spices which are widely used ascomplementaryspicestoaddflavoranddelicacyoffood.Onioncontainflavonoidcompoundsthatareusefulinthetreatmentofvariousdiseasessuchascough,ulcer,flatulence,hypertension,seizuremedicationetc.It’scanalsobeusedasanantibacterial,antiinflammatory,antioxidantorantibiotic.Redonionskinwasteisalsowellknowntocontainactivecompoundssothatitiscommonlybeusedastraditionalmedicine.Thequercetinfromthebarkextractisalsoknowntohaveantiinflammatoryactivity. Flavonoid compounds in plants can be obtained through both conventional ormodernextraction.Thisstudyaimstodeterminethelevelsfromflavonoidextractsofonionskinobtainedby using Micowave Assited Extraction(MAE)extractionand its antibacterial activity againstStaphylococcus aureus(S.aerus)by using variation concentration of 20, 40, 60, 80 and 100 %w/v.FlavonoidlevelsweremeasuredbyusingUVSpectrophotometerat431nmwavelength,andtheantibacterialactivityofonionbarkagainstS.aureuswasdetermineddiameterofbacterialgrowthinhibitionzones(LDH)inagardiffusion.Variationofconcentrationusedwere20,40,60,80and100%w/v.TheextractionofredonionskinusingtheMAEmethodobtainedanaverageextractyieldof9.79%andflavonoidlevelsof14.57%.Theinhibitionzoneofextractatconcentrations20%,40%,60%,80%and100%were18.00mm,19.50mm,19.50mm,22.00mmand21.50mmrespectively.Keywords:Onionskin,MAEmethod,Staphylococcusaureu


185


INSILICOSTUDYOFPYRAZOLYLAMINOQUINAZOLINETOXICITYBYLAZAR,PROTOX,ANDADMET

PREDICTOR

SupandiSupandi*,Yeni,FajarMerdekawatiDepartmentofPharmacy,FacultyofPharmacyandScience,UHAMKAJakarta,13460,Indonesia


ABSTRACTPyrazolylaminoquinazoline is obtained from syntheticAZD4547 and can inhibit kinase activity inrecombinant fibroblastgrowthfactorreceptor (FGFR) invitro.Theobjectiveof thisstudywastoobtain high activity and low toxicity pyrazolylaminoquinazoline derivatives in silico. The 2-dimensionalstructuresweregeneratedusingtheChemDrawapplication.TheLazarapplicationwasused to predict endpoint carcinogenicity, maximum daily dose, and mutagenicity. The ProToxapplicationwasusedforendpointLD50andtoxicityclasses,whiletheADMETapplicationwasusedfor endpoint hepatotoxicity, with reproductive system disorders, and endocrine. Based on thescoringfromthethreesoftwareapplications,twocompoundswereidentifiedasbeingactiveagainstFGFR2,withnocarcinogenicortoxiceffectsontheliver,endocrinesystem,andthereproductivesystem,buttheywerepredictedtohavemutageniceffects.ThesecompoundswereV29(N-(5-(3,5-dimethoxy phenethyl -1H-pyrazol-3-yl)-7(octahydro-2H-pyrido[1,2-a]pyrazine-2-yl) quinazoline-4-amine), with an IC50 of 0.2 ± 0.1 nM and a toxicity score of 1027, and V32 (N-(5-(3,5-dimethoxyphenethyl)-1H-pyrazol-3-yl)-7-(4-(dimethylamino)piperidine-1-yl)quinazoline-4-amine),withanIC50of0.3±0.1nMandatoxicityscoreof1024.Keywords:Insilico,Pyrazolylaminoquinazoline,Lazar,ProTox,ADMETPredictor.


186

PosterPresentation [PP-89]BiologicalactivityandtoxicityofacetoneextractsandsecondarymetabolitesfromSoutheast

Sulawesisponge,Chlatriasp.

SahidinIdin1*,SadarunBaru2,Wahyuni,1RiniHamsidi1,AjengDiantini3,JuttiLevita3

1FacultyofPharmacy,UniversitasHaluOleoKendari93232Indonesia

2FacultyofFisheriesandMarineScience,UniversitasHaluOleo,Kendari93232,Indonesia3FacultyofPharmacy,UniversitasPadjadjaran,Sumedang,Indonesia


ABSTRACTFoursteroidshavebeenisolatedandidentifiedfromacetoneextractsofChlatriaspspongethatarechlatruhoate (1), 3β-(acetoxymethyl)-A-nor-5α-cholestane (2), 3β-(hydroxymethyl)-A-nor-5α-cholest-15-ene (3), and 3β-(hydroxymethyl)-A-nor-5α-cholestane (4)1. To explore the potentialbiologicalactivitiesofacetoneextractsandisolatedcompounds,theactivityagainstthebacteria,radical scavenger and toxcicity were tested. The tested bacteria includes Escherichia coli ATCC35218,SalmonellathypiiYCTC,ShigelladysenteriaeATCC13313,Bacilussubtilis,andStaphylococcusaureusATCC 25923. The test of radical scavenger used DPPH, and toxicity using Brine shrimpLethalityTest (Artemiasalina).The results showed that the theacetoneextractsgenerally showweakactivityagainsttestedbacteriawhereaspureisolatesarenotactiveagainsttestedbacteria.Boththeacetoneextractandtheisolatedcompoundexhibitedweakactivityasradicalscavengerand not toxic toward Artemia salina.The relationship among the isolated compounds can beproposedasfollows:compound1isderivedfromesterificationofcompound3withpropanoicacid,thecompound2isesterificationproductofcompound3withaceticacidandcompound3istheoxidationresultofcompound4.Keywords:antibacterial,Chlatriasp.,radicalscavenger,steroids,toxicity


187


BIOCHEMICALCHANGESINTHECOMBINATIONOFCAPTOPRILWITHCELERYEXTRACTINHYPERTENSIVERATSINDUCEDSODIUMCHLORIDE

SiskaSiska1,2,*AntonBahtiar2,AbdulMun`im2,FranciscusD.Suyatna3

1FacultyofPharmacyandScience,UniversitasMuhammadiyahProf.Dr.HAMKA,EastJakarta,13460,Indonesia

2FacultyofPharmacy,KampusUIDepok,UniversitasIndonesia,WestJava,16424,Indonesia3DepartementofPharmacologyandTherapeutics,FacultyofMedicine,Universitas

Indonesia,CentralJakarta,10430,Indonesia*Email:[email protected]

ABSTRACT

Celery (Apiumgraveolens L.) is an edibleherb that usually used as an antihypertensive. In theprevious research, there was evidence that celery extract influenced the pharmacokinetics ofcaptopril1. This researchaims to study thebiochemical changes inhypertensive rats thathavebeengiventhecombinationofcaptoprilandceleryextractorally.Spraque-Dawleystrainratsweredivided intoninegroups(n=5).Group I:normalcontrol; II:negativecontrol; III:positivecontrolgroup(captopril1.25mg/kgBW);IV:positivecontrol(captopril2.5mg/kgBW);V:positivecontrol(captopril5mg/kg);VI:celeryextract(40mg/kg);VII:combinationofcaptopril1.25mg/kg+extract;VIII:combinationofcaptopril2.5mg/kg+extract;IX:combinationofcaptopril5mg/kg+extract.Theresultsshowedthatthecombinationofcaptopril(5mg/kgBWwithceleryextract(40mg/kg BW) decreased the blood pressure 42.34% (p <0.05) when compared to the singleadministrationofcaptopril(5mg/kgBW).Increasesoccurredinthelevelsofpotassiumurine,urinesodium,andserumcreatinineinthecaptoprilcombinationofceleryextractswhencomparedwiththesinglecaptoprilgroup.Urinarypotassiumlevelsincreasedinallcombinationgroupsbutwerenot significantlydifferent compared to the singledosecaptopril group (p>0.05).Urine sodiumlevelsingeneralincreasedinalltreatmentgroupswiththelargestincreaseoccurredingroupIXsignificantly compared to the single group of captopril of the same dose (p < 0.05). Serumpotassiumelectrolytelevelsdecreasedinallcombinationgroupsbutnotsignificantlydifferent(p>0.05) when compared with the single captopril group the same dose. The administration ofcaptopril,extractandthecombinationdidnotaffectkidneyandliverfunctionwhencomparedtothenormalgroup(P>0.05).Inconclusion,thecombinationofcaptopril(5mg/kgBW)andceleryextract(40mg/kgBW)canreducebloodpressurebetterthancaptoprilaloneinthesamedosesandcanalterthebiochemicalchanges.Thecombinationmightbebeneficialforthetreatmentofhypertension,ascelerycausesadecreaseinbloodpressure.Keywords:ApiumgraveolensL.,captopril,combination,interaction,biochemical


188


ADVERSEDRUGREACTIONSPROFILEOFFISTLINEANTITUBERCULOSISDRUG(STUDYONINDONESIANPHARMACOVIGILANSDATABASE)

SetyoUtami*,YunitaNita,UmiAthiyah

FacultyofPharmacyAirlanggaUniversity,Jl.DharmawangsaDalamSurabaya60286


ABSTRACTTheADRsprofilesidentificationofantituberculosisdrugsisusefulforearlywarningoftheriskthatmayarisedue todrugsuses.The researchmethodused isa retrospectivewitha sample reportcontainedintheIndonesianpharmacovigilansdatabaseavailableonlineate-meso.pom.go.id.Datais taken from 2014 to May 2018. The aim of this research was to identify ADRs form 1stlineantirtuberculosisdrugsthatsreportedonIndonesianPharmacovigilansDatabase.Theresultsofthisstudyarefirst-lineOATADRswhicharethemostADRsreport(69,3%),mostADrsareskinreaction(33%).Keywords:ADRs,Tuberculosis,Pharmacovigilans


189


POTENTIALMEDICATIONERRORINELECTRONICPRESCRIPTIONATPRATAMAHEALTHCAREIN

BANDUNG

RizkiSitiNurfitria*,DeniIskandar,RimaNurAdillahEffendi

SekolahTinggiFarmasiBandung,Jl.SoekarnoHatta754Bandung40191*Email:[email protected]

ABSTRACT

Aneffort to reducemedicationerrors is tousedrugsprescriptionelectronically.However, thereneedstobeanevaluationofthissystem,becausethissystemisnotfreefromerrorsintreatment.Thisstudyaimedtofindoutthedescriptionofprescribingflow,toexamineelectronicprescriptioncompleteness,andfindoutthepotentialmedicationerrorsthatoccurintheprescribingphaseofelectronicprescriptionusingprescribingindicators.ThisstudyusedobservationalmethodbytakingoutpatientprescriptiondataofMarch2018inahealthserviceandanalyzeddescriptively.Electronicprescriptionofmedicinesat thishealthcarewasmadeand inputtedbydoctors.Whenanerroroccuredonacomputersystem,thedoctorwouldprescribeitmanuallysothatthepatientcanstillbe served. Therefore, the use of prescribing indicators was used in the analysis of potentialmedicationerrors.Incompletenessofthemostcommonrecipewasonadministrativerequirementswhereallprescriptionsdidnotlistedthedoctor'spracticepermit,patient'sgender,patient'sweight,telephonenumberoftheplaceofpractice,andpatient'scontactnumber.MedicationerrorshadthemostpotentialfortheoccurrenceofprescriptionwritingwithtwoormoredrugsinteractingandthiserrorwasclassifiedascategoryDaccordingtoNCC-MERP.Keywords:electronicprescribing,medicationerrors,prescribingindicators


190


LOOP-MEDIATEDISOTHERMALAMPLIFICATION(LAMP)FORRAPIDDETECTIONOF

Salmonellaspp.INTRADITIONALMEDICINEPRODUCTS

SoniMuhsinin*,RahmaZiskaBandungSchoolofPharmacy,Jl.SoekarnoHattaNo.754Bandung40614.


ABSTRACTTraditional medicines in the form of chopped, simplicized powder and other dosage forms arepharmaceutical products that are often contaminated with microbial pathogens, such asSalmonellasp.Conventionalcontaminationtesting,thatisbyculture,hasdisadvantagesincluding:longtime,difficultyinworking,laborius,andrequiresastandardlabwithahighlevelofbiosafety1.Research has been carried out in the development of diagnostic kit for the rapid detection ofSalmonella sp.on traditionaldrugpreparationsusing the LAMPmethod.MoleculardetectionofSalmonellaspp.basedonLAMPmethodsisafasterandsimplerapproachthanconventionalculturemethods. The research method was carried out starting from DNA isolation of samples, LAMPprimarydesign,methodof spikeon traditionaldrugpreparations,andLAMP.The resultsof thisstudyshowedthatallsamplesoftraditionaldrugsthathadbeencontaminatedwithSalmonellaandpositivecontrols (SalmonellaATCC) showedpositive results.Whereas traditionalmedicine that isnot contaminated and negative control (Nuclease free Water) shows negative results. LAMPdetectionresultsarethesameastheresultsofSalmonellacultureasagoldstandard.TheLAMPmethodcandetectSalmonellaspp.ontraditionalmedicinalproductswithasensitivityandspecificityvalueof100%.Keywords:LAMP,Salmonella,diagnosticKIT,TraditionalMedicine


191


STUDYTHELEVELOFKNOWLEDGEOFPREGNANTWOMENABOUTNON-PRESCRIPTIONDRUGS

ANDTHEIRUSEBEHAVIOR

NiNyomanSriMasHartini*,RizkiSitiNurfitria,IndahFebriyantiAmir

BandungSchoolofPharmacyJl.Soekarno-HattaNo.754Bandung-Indonesia


ABSTRACTTheIndonesianCentralStatisticsAgencystatedthatinthelastamonthin2014around20.99%ofIndonesianschosetodoselfmedication.AstudyinNigeriastatedthat,asmanyas375(72.4%)of518pregnantwomenwhowerestudieddidself-medication.Thisstudyaimstodeterminethelevelof knowledge, description of usage, usage behavior and the relationship between the level ofknowledgeofnon-prescriptiondruguseinpregnantwomenwiththeirusebehavior inJatimulyovillage,SouthLampungRegency,LampungProvince-Indonesia.Thisstudyisanobservationalstudywith a cross-sectional approach and the data are presented in descriptive quantitative andqualitative.Theresultsshowedthat56(59.57%)ofthetotal94pregnantwomenrespondentsusednon-prescriptiondrugs.Themostwidelyusednon-prescriptiondrugsareparacetamol(28.57%).Thelevelofknowledgeof theuseofnon-prescriptiondrugs inpregnantwomen is in thequitegoodcategory(68.95%).Thelevelofbehaviorofnon-prescriptiondruguseinpregnantwomenisinthegood category (78.53%). There is a positive relationship between the level of knowledge andbehaviorofnon-prescriptiondruguseinpregnantwomenwithasignificancevalueof0.001.Keywords:pregnantwomen,non-prescriptiondrug,selfmedication


192


COMPARATIVEDPPHSCAVENGINGACTIVITYANDCYTOTOXICITYAGAINSTMDA-MB-231CANCERCELLSOFAbelmoschusesculentusANDAbelmoschusmoschatusLEAFEXTRACTS

DianRatihLaksmitawati1*,CristyFlorenciaThi1,ErinaClaudya1,WahyuWidowati2

1FacultyofPharmacy,PancasilaUniversity,JalanSrengsengSawah,Jagakarsa,Jakarta-12230

2FacultyofMedicine,MaranathaChristianUniversity,JalanSuryaSumantriNo.65,Sukawarna,Sukajadi,Bandung-40164*Email:[email protected]

ABSTRACT

The incidence of breast cancer is increasing in developing countries. Herbal medicine is stillinterestingareatostudy.TheleavesofAbelmoschusmoschatusMedik(A.moschatus)havebeenstudiedasanticancerforhumancolorectalandretinoblastomacancercelllineswhereastheflowersofAbelmoschusesculentusL.Moench(A.esculentus)showanticanceractivityagainsthumanliverHepG2celllines.Theaimofthisstudyistoevaluatethepotentialantioxidantandanticanceractivityof Abelmoschus esculentusL. Moench (A. esculentus) and Abelmoschus moschatusMedik (A.moschatus) leavesextract.AntioxidantactivityofbothextractswasmeasuredusingDPPHassaywhile MTS assay was done to show anticancer activity of both extracts in MDA-MB-231.Phytochemicalscreeningandtotalflavonoidcontentwereconductedandcalculatedasquercetin.ThisstudyshowedthatA.esculentusandA.moschatushavemoderateandhighDPPHscavengingactivityvalueofIC50114.09µg/mland74,88µg/mlrespectively(P0.002<0.05).While,cytotoxicassayofA. esculentusandA.moschatusinMDA-MB-231displayedweakly andmoderatelyeffectwithIC50valueof254.77µg/mland132.98µg/mlrespectively(P0.113>0.05).Thesamemetaboliteshave been found in both extracts using phytochemical assay. Coumarin is only found in A.esculentusand the essential oil is only found in A. moschatus. Total flavonoid content ofA.esculentus(0.45±0.05%)andA.moschatus(0.38±0.06%)werenotsignificantlydifferent(P0.206>0.05).Inconclusion,cytotoxicactivityofbothextractswasnotsignificantlydifferent(P0.113>0.05),eventhoughbothantioxidantactivitiesweredifferent.Keywords:Antioxidant,Anticancer,AbelmoschusesculentusL.Moench,AbelmoschusmoschatusMedik


193


ToxicityTestOfMahoniEthanolExtratc(Switeniamahagoni)

VirsaHandayani*,AhmadNajib,Reskiamriati,Lahamidu,AktsarRoskianaAhmad

UniversitasMuslimIndonesia,Jl.UripSumoharjokm.590214MakassarIndonesia


ABSTRACT

Mahoniisamedicalplantwhichhavethepotentialasdrug.Theaimsofthisresearchweretoanalysisphytochemical content and to test the toxicity of ethanol extract of seed from Mahoni. ThePhytochemicalsthatanalyzedweretotalphenolic,totalflavonoidandcondensedtannin.ToxicitytestwasassessedusingBSLTmethod.Extractionwasdonebymaserationmethodusingethanolasthesolvent. InBSLTmethod, theshrimp larvaewereplaced inaseriesof testsolutionofvariedconcentration. The value of LC50were obtained based on calculation of shrimp larvae lethalitypercentageusingprobitanalysis.LC50valuesofethanolextractwere0,95ppm.Keywords:mahoni,toksisistas


194


THEEFFECTOFHClCONCENTRATIONINTHEHYDROLYSISPROCESSOFNATADECASSAVAON

THECHARACTERISTICSOFMICROCRYSTALLINECELLULOSE

Prasetia,IG.N.JemmyA*,CokordaIstriSriArisanti,KadekMegayanti,RahayuWirayanti,NiLuhPutuAnitaPratiwi

ProgramStudiFarmasi,FMIPA,UniversitasUdayana,Jimbaran,Bali-80361


ABSTRACTNataisafermentedfoodproductwhichproducedfrombacteriumAcetobacterxylinum[1][2].OnetypeofnataisNatadeCassava.Thiscanbeobtainedfromtheutilizationoftapiocaflourindustrialwaste.Cellulosecontentinnatais[3][4]82.37%.Thishasthepotentialtobecomearawmaterialformicrocrystallinecellulose.Thepuritylevelofmicrocrystallinecelluloseisbasedonthecontentofalphacellulose.Thisisinfluencedbytheprocessofhydrolysiswithacidicsolutions.ThemethodofproducedmicrocrystallinecellulosefromNatadeCassavawascarriedoutbyhydrolysisusingHClatvariousconcentrations(0.5N;1.5N;2.5N;3.5Nand4.5N)[5].[6]Theaimsofthisstudywastodetermine theeffectof variations inHCl concentrationon thecharacteristicsofmicrocrystallinecellulose from Nata de Cassava. The examined parameters were organoleptic, flow properties,Carr’sindex,alphacelluloselevels,XRDandSEM.TheresearchconcludedthatthevariationofHClconcentrationinthehydrolysisprocesshadasignificanteffectonalphacelluloselevels.However,itdidnothaveasignificanteffectonorganoleptic,flowproperties,Carr’sindexandalsoXRDandSEManalysis.ThebestcharacteristicsofmicrocrystallinecellulosewereobtainedinconcentrationofHCl4.5N[7][8].Keywords:microcrystallinecellulose,NatadeCassava,Hydrolysis,HCl


195


ISOLATIONANDANTIOXIDANTACTIVITYOFSECONDARYMETABOLITESFROMPIRDOTLEAVES

(SaurauiavulcaniKorth.)

YumEryanti,Yuharmen,RudihendraSy,NilaSariBrTompul

DepartmentofChemistry,FacultyMathematicsandNaturalScienceUniversityofRiau,Pekanbaru,28293,Indonesia


ABSTRACT

Pirdot (S. vulcani Korth.) is a species of family Actinidiaceae. This family contains many activecompounds that have activities such as anti-diabetic,anti-oxidant, anti-tumor, anti-fungi and anti-depressant. Pirdot leaves used to treat by NorthSumatran,likediabetic,gout,cancer,cholesterolandskindiseases.Phytochemicaltestshowedthepresenceofsecondarymetabolismintheformofflavonoid,phenolicandsteroid.Inthisstudy,theanti-oxidanttestwasperformedby1,1-diphenyl-2-picrylhydrazyl(DPPH)methodonethylacetateextractanditspurecompound.Isolationwasheldbymacerationusingmethanol.TheextractwasseparatedbyVLCusingagradientsolventandyielding5fractions.Isolationattheincorporationoffractions 3 and 4 yields a pure compound codenamedP-F34 in the form of an orange-colored crystal having a melting point of184-185oC.Theanti-oxidanttestofethylacetateextractandP-F34compoundisknowntoactivelyinhibitDPPHradicalatconcentrationsof49.85μg/mLand8.70μg/mL.Structuralcharacterizationwas performed by UV, IR, NMR andLC-MSspectroscopy.DatafromUV,IR,NMRandLC-MSspectrashowedthatP-F34compoundisaflavonoidcompoundtypeflavonolthatbindtosugaroframnosa,thatisquercetin–3–O–rhamnosidehaving 7 hydroxyl group (R–OH),3 ether group (R–O–R’), 1 ketone carbonyl group (–C=O), 10 tertiary carbon atoms (–CH), 9quaternarycarbonatomsandprimarycarbonatom(–CH3)whichistypicalsignalofrhamnoside.Keywords:antioxidant,DPPH,isolation,pirdotleaves


196


THEACTIVITYTESTOFACETONE,METHANOLANDETHANOLEXTRACTOFMoringaoleiferaLEAF

ASPHOTO-PROTECTIONANDCOLLAGENASEINHIBITOR

NiningSugihartini1,SaptoYuliani1,MochammadSaifulBachri1,DessyErlianiMugitaSari2,AyuWulandari2,IinSuhesti2,

1FacultyofPharmacyUniversitasAhmadDahlanJln.Prof.Dr.SoepomoJanturanUmbulharjoYogyakarta

2StudentofPascasarjanaProgramFacultyofPharmacyUniversitasAhmadDahlanJln.Prof.Dr.SoepomoJanturanUmbulharjoYogyakarta*Email:[email protected]

ABSTRACT

Moringa leaf extract can be used as antiaging and sunscreen1-5. The solvent used during theextractionprocesswilldeterminetheamountofactivesubstanceanditsactivity.ThereforetheaimofthisstudywastodeterminetheeffectofsolventtypesonextractionofMoringaoleiferaleaves on the content of the active substance and its activity as a sunscreen and collagenaseinhibitor.Inthisstudy9typesofsolventswereusedwhichincludedacetone,methanolandethanolwith concentrations of each solvent : 50%, 70% and 96%. β-carotene, phenolic and flavonoidcontent of each solvent usedwas determined. After that, the activity of photo-protectionwasdeterminedbycalculatingtheSunProtectionFactorvalueandantiagingactivitybymeasuringtheinhibitory activity of the collagenase enzyme. The test results showed that the highest solventcontained β-carotene, phenol and flavonoid were 96% ethanol, 70% acetone, 96% acetone,respectively.SolventswiththehighestnumberofSunProtectionFactorvaluewas96%ethanolandthehighestcollagenaseinhibitionwasshowedby70%acetoneextract.Basedonthetestresultsitcanbeconcludedthatthetypeofsolventaffectedtheactivesubstancecontentanditsactivityasasunscreenandinhibitorofcollagenaseenzymeactivity.Themostoptimalsolventisacetone70%.Keywords:acetone,ethanol,methanol,Sunprotectionfactor,collagenase


197


POTENCYOFANGIOPTERISspp.EXTRACTSASANTIOXIDANT,ANTIBACTERIAL,INHIBITOROF

ACETYLCHOLINEESTERASE,ANDBETA-GLUCOSIDASE

AhmadFathoni1*,Praptiwi1,DewiWulansasi1,HiroshiKamitakahara2,ToshiyukiTakano2andAndriaAgusta1

1NaturalProductLaboratory,ResearchCenterforBiology,IndonesianInstituteofSciences2GraduateSchoolofAgriculture,KyotoUniversity,Kitashirakawa-oiwake-cho,Sakyo-ku,Kyoto

606-8502*Email:[email protected]

ABSTRACT

Angiopterisspp.,locallyknownaspakukebo,pakugajah,hatitanah,andpakismundingcommonlyusedastraditionalmedicineinIndonesiatotreatfever,hepatoprotector,andmalaria.ThepresentstudywasaimedtorevealthepotencyofAngiopterisspp.asantioxidant,antibacterial,inhibitorofacetylcholineesterase,andbeta-glucosidase.Theplantsamples,Angiopterisevecta,Angiopterissp.andA.angustifoliacollectedfromWestJavaandSumbaIsland(EastNusaTenggara);WestSumateraandPapua;andWestJava,respectively.Thefreshleaves,stem,andtubersoftheplantsweredriedby freeze dryer or under sunlight. The dry samples were extracted bymaceration in 90-100%acetone. In vitro antioxidant, antibacterial, acetylcholinesterase inhibitor, and beta-glucosidaseinhibitorwas carriedout by Thin Layer Chromatography (TLC) bioassay. The total phenolic andflavonoid were also determined by using UV-VIS spectrophotometer. The value of MIC asantibacterialactivity, thevalueof IC50ofDPPHradical scavengingandAntioxidantActivity Index(AAI)weredeterminedusingmicroplatedilutionassay.Results:BasedonTLCbioassayshowedthatthe extracts have bioactivity as an antioxidant (10 extracts); antibacterial against S.aureus (12extracts),andE.coli(3extracts);acetylcholineesteraseinhibitor(7extracts);andbeta-glucosidaseinhibitor(11extracts).ThetotalphenolicandflavonoidweredeterminedasmgGAEandQEpergextract.TheMICvalues,IC50ofDPPHradicalscavengingactivityandAAIvalueswillbepresented.Theresults indicatethatAngiopterisevecta,Angiopterissp.andA.angustifoliaarepotentialplantcandidates for thedevelopmentof antioxidant, antibacterial, acetylcholinesterase inhibitor andbeta-glucosidaseinhibitoragentsderivedfromplants.Keywords: Angiopterisspp., antioxidant, antibacterial, acetylcholineesterase inhibitor, beta-glucosidaseinhibitor


198


INDUCTIONOFEPITHELIALOVARIANCANCERBYIMPLANTATIONOF7,12-dimethylbenz(a)athracene(DMBA)COATEDSILKINTHEOVARYOFRATASANIMALMODELFOR

STUDYANTICANCERDRUGSINVIVO

NiMadeDwiSandhiutami1,2*,WawaimuliArozal3*,MelvaLouisa3,PuspitaEkaWuyung4,MokhamadFakhrulUlum5

1DoctoralPrograminBiomedicalSciences,FacultyofMedicine,UniversitasIndonesia,

Jakarta,Indonesia2FacultyofPharmacy,UniversityofPancasilaJakarta,Indonesia

3DepartmentofPharmacologyandTherapeutics,FacultyofMedicine,UniversitasIndonesia,Jakarta,Indonesia

4DepartementofAnatomicalPathology,FacultyofMedicine,UniversitasIndonesia,Jakarta,Indonesia

5DepartementofReproductionandPathologyClinic,FacultyofVeterinary,BogorAgriculturalUniversity


Ovariancancerisagynecologicalcancerthatthefifthmostcommoncauseofdeathsintheworld.Four from five ovarian cancer’s patients is diagnosed at advanced stage and already spread toabdomen.Therapeuticmodalitiesforovarianepithelialcancerarecytoreductivesurgeryfollowedbyplatinumcombinationchemotherapy(cisplatinorcarboplatin)withtaxanandphytopharmaca.Detectionandeffectivetreatmentforovariancancerisstillaclinicalchallenge.Becausetheresultofovariancancertreatmentarestillfarfromoptimal,developingrelevantanimalmodelforovariancancerarestillneededtostudyanticancerdrugsinvivo.Thisresearchaimtogettherelevantanimalmodel for epithelial ovarian cancer andultrasonography approach todetect volumeand sizeofovarian cancer. Induction of ovarian cancer in ratswas carried out using 7,12-Dimethylbenz [a]anthracene(DMBA)coatedonsilksutureandthenimplantedintheleftovaryoffemaleWistarrats.Noduleweredeterminedweeklyafterthefirstoperationbypalpatingtheabdominalwallofrats.Utrasonographywascarriedoutat24thweek.Ratswereeuthanized,ovarianorgansweretakenandhistopathological examinationwas performedwith haematoxylin and eosin staining. Nodules inDMBA-treated rats were noted at 16thweeks post implantation and reaching a cummulativeincidenceof96,4%at24thweeks.Onultrasoundexaminationatthe24thweek,thereweresignificantdifferencesinleftandrightovariansizeandvolume.Thehistologicaltypeofinducedtumorincluded: serous carcinoma cell, clear cell carcinoma, sarcoma and squamous carcinoma. The implanted7,12-Dimethylbenz[a]anthracene(DMBA)coatedsilksutureintheovariesratscanmadeananimalmodel for testing therapeutics agent of ephitelial ovarian cancer and ultrasonography candeterminethesizeandvolumeoftumorsformedinovary.Keywords:7,12-Dimethylbenz[a]anthracene,a nimal model, epithelial ovarian cancer,ultrasonography


199


COMBINATIONKARASINFUTIONANDANTIBIOTICSAGAINSTPATHOGENICBACTERIA

RafikaSari*,PratiwiApridamayanti

DepartmentofPharmacy,MedicineFaculty,TanjungpuraUniversityProf.Dr.HadariNawawiStreet78124*Email:[email protected]

ABSTRACT

FICIvalue(FractionalInhibitoryConcentrationIndex)usedtodescribetheresultoftwocompoundantibacterial in resist bacterial growth so that FICI value characteristic can be known, there issynergic, addictive, indifferent, or antagonist.According to the background above this researchpurposeistoanalyzeFICIvaluefromcombinationofantibioticwithKarasInfusionagainstbacteriaisolatefromulcerdiabetespatientlevelIIIandIVWagner.ThisResearchusingKirby-Bauermethodwithcombinationinfusionwith25%;75%;50%:50%;75%:25%withGentamicin,Tetracycline,andCiprofloxacin.BasedonthisresearchcombinationfromKaras:Gentamicinwith50%:50%showedinhibitionzone(6,23+0,09mm);Karas:Ciprofloxacin50%:50%(6,37+0,21mm),also75%:25%fortetracycline(6,20+0,16)againstBacilluscereus.combinationandagarwoodleafinfusionwiththediameterzoneis50%:50%forGentamicin(6,40+0,08mm)andCiprofloxacin(6,38+0,08mm),also75%:25%forTetracycline(6,27+0,09mm)againstMicrohydrocarbon.CombinationKaraswiththesmallestdiameterzoneis50%:50%withGentamicin(6,42+0,08mm),Tetracycline(6,17+0,05mm),and Ciprofloxacin (6,33+0,12 mm) against Clostridium sphenoides. The last for streptococcuspyogensantibioticcombinationKaraswiththesmallestdiameterzoneis50%:50%forGentamicin(9,50+0,16 mm) and Tetracycline (9,20+0,14 mm), also 75%:25% for Ciprofloxacin (10,45+0,27mm).TheconclutionfromthisresearchareFICIvaluewere2andallhadIndifferentAntibacterialcharacteristicagainstpathogenicbacteria.Keywords:KarasInfusion,Antibacterial,FICI


200


FICIValueDeterminationofCombinationAquilariamicrocarpaBaill.EthanolicExtractwith

AmoksisilinagainstUTIBacteria

PratiwiApridamayanti1*,Robiyanto1,TrieFarica1

1DepartementofPharmacy,FacultyofMedicine,UniversityofTanjungpura,Pontianak,78124,Indonesia


ABSTRACT

Karas(AquilariamicrocarpaBaill.)isaplantthathasantibacterialactivityagainstsomepathogenicbacteria1. Amoxicillin is one of the antibiotics that can be used to treat urinary tract infection.Escherichiacoli,PseudomonasaeruginosaandProteusmirabilisarethebacteriathatcauseurinarytractinfections2.Thecombinationofantibioticandextractthatshowedsynergisticcharacteristicscandecreaseantibioticresistanceandincreaseantibioticactivity.Theobjectiveofthisresearchwasto determine the FICI value of combination ofAquilariamicrocarpaBaill. ethanolic extract withamoxicillinagainstUrinaryTractInfection(UTI)bacteria.MethodusedisKirby-Bauerdiscdiffusionmethod. Data analysis was descriptively to know combination characteristic. The combinationsshowedsynergisticforconcentrationsof0,0156mg/mL(1/4MIC)amoxicillinand0,125mg/mL(1/4MIC)extractagainstPseudomonasaeruginosa,indifferentforconcentration0,0156mg/mL(1MIC)amoxicillinand0,5mg/mL(1MIC)extractagainstEscherichiacoliandantagonistforconcentration0,0156mg/mL(4MIC)amoxicillinand16mg/mL(4MIC)extractagainstProteusmirabilis.Theresultshowed that the FICI value of combination of Aquilaria microcarpaBaill. ethanolic extract withamoxicillin against Pseudomonas aeruginosa (FICI=0,5),Escherichia coli (FICI=2), and Proteusmirabilis(FICI=8). This show that the combination characteristic of bacteria were varied(synergistically,indifferent,antagonist).Keywords:AquilariamicrocarpaBaill.,amoxicillin,FractionalInhibitoryconcentrationIndex(FICI)


201


FORMULATIONANDEVALUATIONOFENTERIC-COATEDASPIRINMICROCAPSULESUSINGACRYL-

EZE®IIBYEXTRUSIONANDSFERONIZATIONMETHOD

SantosoR*.,MuhsininS.,MardhianiY.D,MuzdalifahDBandungSchoolofPharmacy

SoekarnoHattaNo.754,Bandung,40614*Email:[email protected]

ABSTRACT

Extrusionandspheronizationisacombinationofmicroencapsulationmethodsthatcanproducegoodmicrocapsules.Microcapsulesmaybeusedtocontroldrugreleasesuchasentericrelease.The purpose of this researchare to avoid of using toxic organic solvents, to implement simpleequipmentsasextruderandspheroniser,andobtaininggoodentericrelease.Thefirststageofthisresearch is extrusion of 5 formulas then evaluated organoleptic and its water content.Subsequently, spheronization and evaluated organoleptic, moisture content, break angle, flowrate,particle sizedistribution,adsorbtionofactive substanceand recovery. F5was selectedof5formulasspheronizationanditwascontinuedintoentericcoating.TheentericcoatingusedisAcryl-EZE®II.ThecoatingsuspensionisvariedwhichF1,F2andF3arerespectivelysolidconcentrationsare 15%, 20% and 25%. The resulting microcapsules are evaluated by organoleptis, moisturecontent,weightgainanddissolution.TheresultofANOVAtestobtainedbysignificancevalue0.000(P <0.05) which showed significant difference in extrudate, sferoids andmicrocpsul evaluationresult. Based on these results it can be concluded that Acryl-EZE® II and simple technology(extruder,spheroniserandcoater)canbeusedinproducingaspirinenteric-coatedmicrocapsulesbutdelayedrealeseoutofdissolutionrequirementsthatlistedinthemonograph.Keywords:Aspirin,Extrusion,Entericcoating,Microencapsulation,Spheronization


202


SPECTROPHOTOMETRICDETERMINATIONOFFUCOIDANFROMWATEREXTRACTOFSargassum

aquifolium(Turner)C.Agradh

LiliekNurhidayati*,NurAdhyanti

FacultyofPharmacy,PancasilaUniversity,SrengsengSawah,Jagakarsa,Jakarta12640


ABSTRACT

Fucoidanisacomplexpolysaccharidecompoundfoundinbrownseaweedthathasawiderangeofbiologicalactivities.OneofbrownseaweedspeciesisSargassumaquifolium(Turner)C.Agradh.Thefucoidancontentdependonthesourceofbrownseaweed.Theaimofthisstudywastoassessthelinearity, sensitivity,precissionandaccuracyofvisible spectrophotometricmethods toassay thefucoidancontent in thewaterextractofSargassumaquifolium(Turner)C.Agradh.Fucoidanwashydrolyzed by sulphuric acid to form 5-methylfurfural then reacted with cysteine to formfucocysteinecomplexthatgivepaleyelloworgreenishyellowcolour.Theoperatingtimewas25-35minute,thentheabsorptionwasmeasuredin399nm.Linearitywasintherangeof20-140ppmwithcorrelationcoefficientof0.9925.Thelimitofdetectionandlimitofquantitationwere0.49ppmand1.48ppm,respectively.Therelativestandarddeviationwas1.50%andtherecoverywasintherangeof99.62–103.72%.ThefucoidancontentinthewaterextractsamplefromGarutandBantenwere16.79%and12.35%,respectively.Thismethodmeetsthevalidationrequirementsi.elinearity,precissionandaccuracy.Keywords:methodvalidation,visiblespectrophotometry,fucoidan,Sargassumaquifolium(Turner)C.Agradh


203


Developmentandmethodvalidationof6-thioguanineand6-methylmercaptopurinein

erythrocytesusingLC-MS/MSDewiSelvinaRosdiana1*,RiantoSetiabudy1,SeriyatiNaibaho2,MelvaLouisa1*

1DepartmentofPharmacologyandTherapeutics,FacultyofMedicine,UniversitasIndonesia,Jakarta,Indonesia

2PharmaMetricLabs,Jakarta,Indonesia*Email:[email protected]

ABSTRACTPreviousstudieshadshownthatthesafetyof6-mercaptopurineinacutelymphoblasticleukemia(ALL) patientswasmainly determinedby the concentrations of itsmetabolites in erythrocytes,whichare6-thioguanine(6-TGN)and6-methylmercaptopurine(6-MeMP).Theaimofthisresearchis to develop and validate a method to quantify concentrations of 6-thioguanine and 6-methylmercaptopurineinerythrocytesandapplythevalidatedmethodinALLpatientsundergoing6-mercaptopurine treatment.We develop amethod to determine 6-TGN and 6-MeMP on LC-MS/MSsystem,usinga1.8µmcolumn(2.1x50mm).Mobilephaseusedforanalysiswereformicacid inacetonitrileandformicacid inH2O(60:40).ExtractionfromerythrocyteswasdoneusingNH4OH in acetonitrile. Bisoprolol was used as internal standard. The method validation wasperformedaccordingtotheEuropeanMedicinesAgency(EMEA)guideline.Detectionfor6-TGN,6-MeMP and bisoprolol were done using ion transitions, m/z 168.08>151.02, 167.1>152.06 and326.12>116.03,respectively.Thecalibrationcurveswerelinearovertheconcentrationrangeof9.9–1979ng/mLfor6-TGNand10–2000ng/mL for6-MeMP.Theothervalidationcriteria,whichconsists of selectivity, accuracy, precision, lower limit of quantification, carry over, dilutionintegrity,matrixeffectandstability,wereachieved.Concentrationsof6-TGNand6-MeMPinALLpatients underwent 6-MP treatment were found in the range of 21.05 – 109.56 pmol/8x108

erythrocytes and 13.06 – 80.27 pmol/8x108 erythrocytes, respectively. The present LC-MS/MSmethod is valid for the quantification of 6-thioguanine and 6-methylmercaptopurine inerythrocytesandcanbeappliedinALLchildrentreatedwith6-MP.Keywords: 6-mercaptopurine,6-thioguanine, 6-methylmercaptopurine, LC-MS/MS, AcuteLymphoblasticLeukemia


204


ANTIBACTERIALACTIVITYOFPEPAYALEAF(CaricapapayaLinn.)EXTRACTAND

FRACTIONSAGAINTSMethicillinResistantStaphylococcusaureus(MRSa)CLINICALISOLATESANDStaphylococcusepidermidisCLINICALISOLATES

TianaMilanda*,WafaMufiedahMaulanyandYoppiIskandar

FacultyofPharmacy,UniversitasPadjadjaran

JalanRayaBandung-SumedangKm21Jatinangor*email:[email protected]

ABSTRACT

Acneisaninflammationofthepolysebaceousglands,includingMethicillinResistantStaphylococcusaureus (MRSa)andStaphylococcusepidermidisbacterial infection.Papaya leaves (CaricapapayaLinn.)areoneoftheherbswhichtraditionallyusedtotreatacne.ThisstudyaimstodeterminetheantibacterialactivityofpapayaleafextractandfractionsagaintsMRSaandS.epidermidisclinicalisolates.Theresearchwascarriedoutthroughplantdetermination,simpliciaextraction,parameterextract examination, antibacterial activity testing of the extract, fractination of the extract,antibacterialactivitytestingofthesefractions,determinationofMICandMBCofthemostpotentialfractionandscreeningphytochemicalsoftheextractandthemostpotentialfraction.Theresultsshowedthatethanolextract,n-hexanefraction,ethylacetatefractionandwaterfractionofpapayaleafhadantibacterialactivityagainstbothbacteria,exceptn-hexanefractionagainstMRSaclinicalisolates.Thewaterfractionwasthemostpotentialfractionwith10%(b/v)MICforMRSaclinicalisolates and1.25% (b/v) for S. epidermidis clinical isolates and20% (b/v)MBC forMRSa clinicalisolates and 2.5% (b/v) for S. epidermidis clinical isolates. Compounds that act as antibacterialsubstancesinethanolextractandwaterfractionofpapayaleavesareflavonoidsandpolyphenols.Keywords: Papaya leaves, Carica papaya Linn., Methicillin Resistant Staphylococcus aureus,Staphylococcusepidermidis


205


EFFECTSOFDIFFERENTPARTSOFREDGUAVA(PsidiumguajavaLinn.)ONSUPEROXIDE

DISMUTASEANDCATALASEACTIVITIES

1AfiantiSulastri*,2A.A.Soemardji,3Sukrasno,and4Amaliya

1,2,3SekolahFarmasi,InstitutTeknologiBandung,Bandung,40252,Indonesia1FakultasPendidikanOlahragadanKesehatan,UniversitasPendidikanIndonesia,Bandung,40154,

Indonesia4FakultasKedokteranGigi,UniversitasPadjadjaran,Bandung,40252,Indonesia


ABSTRACTOxidativestressduetofreeradicalscancausevariouscelldamageanditscomponentsinside,suchasproteins, lipids,andDNA.Theliverplaysasignificantroleinthemetabolicprocess,producesendogenous antioxidant compounds that function in scavenging the free radicals through adetoxificationprocess.Guava is oneof the tropical plantswhich is knownhashigh antioxidantbioactivecompounds.Thisstudyaimtoinvestigatewhichpartofredguavaplanthasthebesteffectrelated to superoxide (SOD) and catalase activities3. The study was conducted throughexperimentaldesignon32malemicewhichdividedintoeighttreatmentgroups,i.e.,oneuntreatedgroupascontrolandsevengroupswhicharetreatedwithvitaminCandvariouspartanddosagesofguavaplantfor14dayslong.Theanimalsweresacrificedonthe15thday,andtheirliverorganswereprocessedintohomogenate.SODandcatalaseactivityweremeasuredbyusingELISAkit.Theresultsshowedthatthemediumripeguavafruithadabetterantioxidanteffectcomparedwiththecontrolandothergroups.Keywords:antioxidant,guava,superoxidedismutase,catalase


206


COSTANALYSISOFTYPE2DIABETESMELLITUSINOUTPATIENTSWITHOUTCOMPLICATION

AkhmadPriyadi,1,2*,DeniIskandar1,DewitaSari2

1,BandungSchoolofPharmacy,Indonesia.2,FacultyofPharmacy,PadjadjaranUniversity,Indonesia.


ABSTRACT

Diabetes mellitus (DM) is a group of heterogeneous metabolic disorders characterized byhyperglycemiawhichmorethan90%ofcasesareDMtype2.Diabetesaffectstheeconomybecauseitisadiseasethatrequireslong-termtreatment,sothatthecostisnotsmall,especiallyforpatientsinLMICduetotheincreasingprevalenceofthisdiseases.Thisstudyaimstodeterminethecostofillness in uncomplicated outpatients in Regional Hospital of Bandung City. This study includedobservational research with cross sectional approach. The data used was primary data whichobtainedfromthequestionnairewhilesecondarydataobtainedfrommedicalrecordsandpatientbills.Determinationofrespondentsinthisstudywerepatientswhoincludedtheinclusioncriteriathathadbeendetermined,thereareuncomplicatedtype2diabetesmellituspatients,outpatients,patientsat the InternalDiseasePolyclinic, EndocrineandMetabolicClinic,patientswith ICD-10E11.9code(uncomplicatedtype2diabetes),agerange35-74years,andthelastarepatientswhohaveavisitatleast4timesduringthemonthsofJanuary-April2018.Aquantitativeanalysiswasperformed todescribe the costof treatment components and statistical analysisusingPearsonCorrelationtoseetherelationshipbetweenthecostcomponentandthetotalcost.Therewas137patient samples thathave secondarydataand81 samplespatienthave secondaryandprimarydata.Theaveragecostofillnesscausedbyuncomplicatedtype2DMdiseaseinoutpatientsbasedonsamplesthathavesecondarydataisRp.277,725.00wherethecostofdrugsandlabshaveasignificantrelationshipwiththetotalcostwhileforthesamplethathavesecondaryandprimarydataisRp.336,113.00permonth.FromthestatisticalanalysisperformedusingSPSSapplication,itcanbeseenthat thecostcomponents thatsignificantlyaffect the totalcostaredrugcosts, labcosts,lossofproductivity,andadministrativecostswithSig.<0.05.Keywords:Costofillness,Type2DiabetesMellitus,PearsonCorrelation


207


OPTIMIZATIONOFNANOPARTICLEPREPARATIONOFCHITOSANHUMANEPIDERMALGROWTHFACTORIONICGELATIONMETHODWITHAPOLYETHYLENEGLYCOLSTABILIZERASACANDIDATE

FORDIABETICULCERMEDICATION

Sriwidodo1*,TotoSubroto2,ImanPermanaMaksum2,BetharyKesumawardhany3,DesakMadeDiahDwiLestari3,Sujatmoko1

1)DepartmentofPharmaceuticsandPharmaceuticalTechnology,FacultyofPharmacy

UniversitasPadjadjaran,Jatinangor45363,Indonesia,+622277962002)BiochemistryLaboratory,ChemistryDepartment,FacultyofMathematicsandNatural

Science,UniversitasPadjadjaran,Jatinangor45363,Indonesia,+622277943913)FacultyofPharmacyUniversitasPadjadjaran,Jatinangor45363,Indonesia,+62227796200


ABSTRACTHumanEpidermalGrowthFactor(hEGF)provedtobeeffectiveboth in-vitroandin-vivointreatingchronicwoundsofdiabeticulcers.TheeffectivenessofhEGFisrelatedtoitsmitogenicpropertiesandtheabilitytoincreaseepithelialcellproliferation.However,diabeticulcerenvironmentcausesthe hEGF to have a low stability, thus requiring either a repeated administration or a highlyprotective dosage form. Chitosan nanoparticles have shown an increased active ingredientsstability.Additionally,themucoadhesivepropertiesofchitosanallowsalongerattachmenttothemucosalwoundsothatitcanenhancetheeffectivenessofthehEGF.Thisstudyaimwastoproducedchitosan nanoparticles formulations containing hEGF using ionic gelation method with sodiumtripolyphosphate(Na-TPP)crosslinkerandpolyethyleneglycol(PEG)asstabilizers.Chitosan-hEGFnanoparticles were formed by the occurrence of ionic interactions between positively chargedaminogroupsinchitosan,withnegativelychargedpolyanionformingasphericalthree-dimensionalstructurethatabsorbshEGFinit.Formulaoptimizationwascarriedouttodeterminethepolymerandcrosslinkercomparisonstobeformulatedintochitosan-hEGFnanoparticleswithPEGvariationsandthenthequalityofthepreparationisevaluated.Theresultsshowedthatoptimalchitosan-hEGFnanoparticles were obtained with 0.1% chitosan composition, 0.15% Na TPP, 400 2 mL PEG.Nanoparticlessizewererangedbetween41.2-117.6nmwith90%sizedistribution,polydispersityindexvaluesreached0.259,andzetapotentialwas+41.29mV.TheformednanoparticlescouldbeseenusingScanningElectronMicroscopy(SEM)andTransmissionElectronMicroscopy(TEM)whichindicatedthepresenceofhEGFproteinencapsulatedbyChitosan-TPP-PEGbiopolymer.Keywords:Chitosan-hEGFNanoparticles, IonicGelationMethod,Tripolyphosphate,PolyEthyleneGlycol.


208

PosterPresentation [PP-114]SimpleandRapidMethodforAnalyzingTamoxifen,Endoxifen,and4HydroxytamoxifeninDried

BloodSpotUsingLiquidChromatographyTandemMassSpectrometry

BaithaPalanggatanMaggadani,TesanikaRibkaJoulinSitorus,CallistaAndinieMulyadi,YahdianaHarahap*

FacultyofPharmacy,UniversitasIndonesia,Depok,Indonesia*Email:[email protected]

ABSTRACT

Tamoxifen(TAM)isusedasstandard-of-careforpremenopausalwomenwithhormonereceptor-positive(ER+)breastcancer.TAMisclinicallyproventoreducebreastcancerrecurrencebyblockingestrogenreceptor,mainlythroughitsactivemetabolites,endoxifen(END)and4-hydroxytamoxifen(4HT),whichhavehigheraffinitytoERthanTAMitself1,2.Asimpleandrapidsamplepreparationand analytical method is needed to quantify TAM and its metabolites for monitoring studiespurposes.TheUPLC-MS/MSmethodwasdevelopedandvalidatedtodeterminethelevelofTAM,END, and 4HT simultaneously in dried blood spot with clomiphene as the internal standard.OptimizationwasdonebyevaluatingseveralparameterthataffectefficiencyofDBSpreparationandanalysisofTAManditsmetabolites.Theresultshowedthatpreparationof20µLbloodspotvolumewith 120minutes of drying time and 25minutes of total extraction timewas themostefficientconditionandalsofulfilledrecoveryandmatrixeffectrequirementaccordingtoFDAandEMAguidelines.TheseparationwasperformedonUPLCClassBEHC18usingformicacid0.1%-formicacid0.1%inacetonitrile(35:65)asthemobilephaseinisocraticmodeat0.25mL/minutewithtotalanalysistimeof4minutes.ThismethodhassuccessfullyfulfilledallvalidationrequirementreferringtoEMA3andFDA4guidelines.Keywords: Tamoxifen, endoxifen, 4-hydroxytamoxifen, chlomiphene, dried blood spot,optimization


209


EFFECTOFSILVERNANOPARTICLESADDITIONINPERIODONTALDRESSINGFORWOUNDTISSUE

HEALING

BudhiCahyaPrasetyo1*,RizkyJuwitaSugiharti2*,IsaMahendra2,IimHalimah2,EvaMariaWidyasari2,NunungRusminah1,IndraMustika1

1PeriodontiaDepartment-PadjadjaranUniversity,SekeloaSelatanI,Bandung40132.

2NationalNuclearEnergyAgency,Tamansari71,Bandung40132.*Email:[email protected],[email protected]

ABSTRACT

Periodontaldressingisaprotectivematerialthatisusedonpost-surgicalperiodontaltoprotectthesurface of thewound and provides a comfort sense to patient. Periodontal dressingmaterialsgenerallydonothaveanyhealingeffectandonlyprotectingtheinjuryside.Silvernanoparticlesisanadditioncompoundinperiodontaldressingthatisexpectedtogiveanaccelerateeffectonthehealing process. This study was conducted to determine the effect of periodontal dressingscontainingsilvernanoparticlesbyevaluatinginflammatoryparametersusing99mTc-ciprofloxacin,aradiopharmaceuticalthatcanbeusedfordiagnosinginfectionandinflammation.Thisresearchwascarriedoutusingpurelyexperimental24maleSpragueDawleyratsanddividedinto4groups.Firstgroup isagroupwithoutany treatment, secondgroupwas thepositivecontrolgroup (excisionprocedureandgivenCoePak(R)), thirdgroupwasnegativegroup(onlyexcisionprocedure),andfourthgroup(excisionprocedureandgivenCoePak(R)withsilvernanoparticles).Theexcisionsitewasconductedonthe5x1mmdextrapalateandclosedusingaperiodontaldressingnoneugenolCoepak(R).Onsecondandfourthdaysaftertheprocedure,eachgroupwasobservedofhealingprocess on the site of excision. To observe the inflammation inside of the excision 99mTc-ciprofloxacinwasinjectedthroughveintoeachgroupandafteronehourtheratwassacrificedanddextrapalateorganofeachratwascountedbySingleChannelAnalyzertocountaccumulationof99mTc-ciprofloxacin.Accumulation99mTc-ciprofloxacindatawasprocessbyasignificancelevelofα<0.05anddescriptivelyusingboxplotdiagram.Afourthgroupshowedatendencyor inclinationdatawhichisgoodingivingeffecttopromotehealingcomparetopositivecontrolgrouponday4withlessaccumulationof99mTc-ciprofloxacinindextrapalate.Intheboxplotdescriptiveanalysisdiagram,thisgroupalsogaveasmallerrangevalueandnooutlierswhichcertainlyindicatesthatadditionofsilvernanoparticlesinperiodontaldressinggaveeffectforwoundtissuehealing.Keywords:Periodontaldressing,silvernanoparticles,woundtissuehealing,radiopharmaceutical,99mTc-ciprofloxacin


210


INCLUSIONBODIESSOLUBILIZATIONOFCEPHALOSPORINACYLASEFROMEschrichiacoli

DudiHardianto1*,BimaWedanaIsdiyono2,JuwartinaIdaRoyani3

1CenterofTechnologyforPharmaceuticalandMedical,AgencyfortheAssessmentandApplication

ofTechnology,LAPTIABBuilding,PuspiptekArea,TangerangSelatan,153142FacultyofLifeSciences,SuryaUniversity,UnityTowerBuilding,BoulevardGadingSerpong,Kav.

M5No.21Street,Serpong,158103CenterforAgriculturalProductionTechnology,AgencyfortheAssessmentandApplicationof

Technology,LAPTIABBuilding,PuspiptekArea,TangerangSelatan,15314*Email:[email protected]

ABSTRACT

CephalosporinC(CPC)canbetransformedto7-aminocephalosporanicacid(7-ACA)bythechemicalandenzymatic reaction.Theenzymatic reactionused two-stepmethodbyD-aminoacidoxidase(DAAO)andGL-7-ACAacylaseandone-stepmethodusedbycephalosporinacylase(CA).Theone-stepmethodisdevelopedbecauseofsimpleprocessandcostreduction.Heterologousexpressionofforeigngenescanproduceabout30%ofsolubleproteinand70%ofinsolubleproteinasinclusionbodies (IBs) in E. coli.IBs are cytoplasmic aggregates of inactive protein and mostly consist ofrecombinant protein. The formation of IBs in E. coliis a challenge for recovery of recombinantprotein in industrial scale. Solubilization and refolding are crucial process to obtain activerecombinantproteinfromIBs.Thepurposeofthisresearchwasoverexpressionandoptimizationofsolubilization process to increase soluble CA from IBs. CA gene was inserted in pET21a(+) andexpressedinEscherichiacoliBL21(DE3)(E.coli).ThecultureE.coliBL21(DE3)wasinoculatedinLuria-BertaniliquidmediumandinducedbyIPTG1mMfor5hours.TheproductionofCPCacylasewastestedeveryhour.TheoptimumofIPTGconcentrationwasdetermined.ForsolubilizationofCPCacylase,thecellsofE.coliweredisruptedbyasonicator.ThepelletofIBswaswashedbytritonX-100andsolubilizedbyureaorguanidineHCl(GdnHCl)andurea.TheE.colicontainingpET21a(+)-acyII expressed CA as soluble and insoluble protein (IBs). The results showed that CPC acylaseproducedbyE.coliBL21(DE3)withoptimumconditionofCPCacylaseproductionwas0.5mMIPTGand optimal induction time of IPTG was 5 hours. The two-step denaturing (2DR) was the bestmethodforsolubilizationIBspellet.Keywords:Cephalosporin,cephalosporinacylase,7-ACA,proteinexpression,Escherichiacoli


211


KNOWLEDGEANDUSAGEOFMEDICINALPLANTSBYLOCALPEOPLE

INWAIGEOISLAND,RAJAAMPAT,WESTPAPUA,INDONESIA

SitiSusiarti*,DebyArifianiandLeberinaK.Ibo

BotanyDivision,ResearchCenterforBiology,IndonesianInstituteofSciencesCibinongScienceCenter,Jl.RayaJakarta-BogorKm46,Cibinong16911,Indonesia


Papuahousedenormousnumberofplantspecies,consistingofabout20,000–25,000species,occurin13developmentzones,includingRajaAmpatArchipelago1.Thearchipelagoconsistsoffourmajorislands,i.e.Salawati,Batanta,MisolandWaigeoislands.LocalpeoplewholiveinthearchipelagoarecalledMayaandMatbat.However,theknowledgeanduseoflocalmedicinalplantshavenotbeencompletelyexplored2.Therefore, the researchaimed to reveal knowledgeaboutmedicinalplantsusedbylocalpeopleinWaigeoisland.Openinterviewanddirectobservationintwovillageswithtwentysourceshadbeenperformed.About40specieswerereportedasmedicinalplantsbylocal people. Some of the species were new addition to the book Inventaris Tanaman ObatIndonesia,otherspeciesarealsousedby localpeople inMoluccasandotherareas inPapuaandPapuaNewGuinea.SeveralspeciesusedbyMayapeopleareAlstoniascholaris,ArcangelisiaflavaandStrychnossp.Keywords:Mayapeople,medicinalplants,RajaAmpat,WestPapua,Indonesia


212


INSILICOSTUDYONS-ALLYLCYSTEINEANDQUERCETINCOMPOUNDFROMGARLIC(AlliumSativum)ASXANTHINEOXIDASEINHIBITOR

AbdulAzizSetiawan1*,ShirlyKumala2,DianRatihL.2,NancyDewiYuliana3

1SekolahTinggiFarmasiMuhammadiyahTangerang,KHSyekhNawawiStreet(RayaPemda)KM.4

No,13,Tigaraksa-Tangerang,Banten157202FacultyofPharmacy,UniversityofPancasila,SrengsengSawahStreet,SouthJakarta12640

3BogorAgriculturalUniversity,DramagaStreet,Babakan-Bogor,WestJava16680*Email:[email protected]

ABSTRACT

Uricacidistheendresultofpurinecompoundscatabolisminthehumanbody.Thenucleotidewillbeoxidizedbythexanthineoxidaseenzymetoformuricacid.Thenormalvalueofuricacidlevelsis3.5to7.0mg/dLformenand2.5to6.0mg/dLforwomen(1).Goutcancauseacuteandchronicarthritis(2).Increasedserumuricacidabovethespecificthresholdasacapitalintheformationofuricacidcrystals(3).Famacologytherapyofgoutusinguricostaticanduricosuricdrugs.Allopurinolisthemostcommonlyusedxanthineoxidaseoruricostaticinhibitorandisprescribedfordecreaseduricacidlevels(4).ThealternativesearchforgoutisneededespeciallyIndonesiaistheSecondMegaBiodiversityCountryafterBrazilwith2500typesofwhicharemedicinalplants(5).OnealternativethatisthoughttoreduceuricacidlevelsbyGarlic(AlliumsativumLinn.).Healthpropertiesofgarlicdependon itsbioactivecompounds,especially theorganosulphurcompounds, thephenolicacidandtheflavonoidconstituents(6).S-allylcysteineandquercetinaspotentanti-gout(7)(8).Theaimofthisstudyistopredicttheinteractionofreceptors,betweentestcompounds.Themethodusedis Autodock 4.2.6.Molecular docking study is a computational method that aims tomimic theinteractioneventofa ligandmoleculewitha targetprotein in invitrotest.Thereceptorused isxanthineoxidasewith1FIQcode.TheinteractionofS-allylcysteineshowstheaminoacidsresidue:distanceofGLU802 is1,906Å,ALA1078 is2,134Å,andfreeenergyofbinding is -4,89kkal/mol,Quercetin shows the amino acids residue: distance of THR1010 is 2,215Å, THR1010 is 1,759Å,GLU1261 is 2,218Å, SER876 is 2,224Å,ALA1079 is 2,144Å, and freeenergyofbinding is -9,53kkal/mol,Allopurinol shows theaminoacids residue:distanceofGLU802 is1,959Å,THR1010 is2,092Å,ARG880is2,140Å,THR1010is2,141Å,andfreeenergyofbindingis-5,75kkal/mol.Theconclusion isQuercetin with freeenergyofbinding is -9,53kkal/molkcal /molpredictedmorepotential as a decrease in uric acid levels in xanthine oxidase receptor because it has a higherdockingscorethanthecomparativeS-allylcysteineandallopurinol.Keywords:Garlic,allopurinol,xanthineoxidase,moleculerdocking


213

PosterPresentation [PP-120]OPTIMIZATIONTRANSGENEEXPRESSIONOFSHORTFULLYSYNTHESIZEDLIPOPEPTIDE-BASED

TRANSFECTIONAGENTFORNON-VIRALGENEDELIVERYVEHICLE

Tarwadi1,2,DewiE.Restiani1,SabarPambudi1,JalalJ.Jazayeri3,ColinW.Pouton4,HeniRachmawati2,5,RahmanaE.Kartasasmita2andSukmadjajaAsyarie2


1CentreforPharmaceuticalandMedicalTechnology-TheAgencyfortheAssessmentandApplicationofTechnology(BPPT),Jl.M.H.Thamrin8Jakarta10340,Indonesia.

2SchoolofPharmacy,BandungInstituteofTechnology,Jl.Ganesa10Bandung40132,WestJava,Indonesia.

3SchoolofBiomedicalScience,FacultyofScience-CharlesSturtUniversity,BooroomaStreet,POBox588,Wagga-Wagga,NewSouthWales2678,Australia.

4VictoriaCollegeofPharmacy-MonashUniversity,381RoyalParadeParkville,VIC3052,Australia.

5ResearchCentreofNanoSciencesandNanotechnology,BandungInstituteofTechnology,Jl.Ganesa10Bandung40132,WestJava,Indonesia.

ABSTRACT

Gene therapy using non-viral gene delivery vehiclemight be applied for the treatment of viralinfection, cancerandgeneticdisorders.However, themost challengingbarrier innon-viral genedeliveryvehicleisthelowefficiencyoftransgeneexpression.Therefore,optimizationoftransgeneexpressionisveryimportantandcriticaltobeperformedbeforedoingextensiveevaluationofthetransfectionagentfornon-viralgenevehicle.Herein,wepresenttransgeneexpressionoptimizationdataofgeneencodingluciferasecomplexedwithaseriesofshortfullysynthesizedlipopeptideonCOS7,HeLaand293Tcells.Theeffectof complex formationDNA-transfectionagent indifferentionic strength of the solvent on transfection efficiency was evaluated. The molar ratio, forcedsedimentation,incubationperiodoftheDNA-transfectionagentcomplexeswerealsoexploredontransfectionefficiency.Moreover,theenhancementeffectontransfectionefficiencyofpolymerPEIas co-transfection agent in DNA-lipopeptide was also investigated. The results show that thecomplex particles of the DNA-lipopeptide were formed efficiently in the low ionic strengthenvironment(HEPESGlucoseBufferpH7.4),andthecomplexwasincubatedmorethan10hoursbefore it was transfected onto the cells. Depending on the lipopeptide, the DNA-lipopeptideincubationin24hours(at4-6oC)resultedanincreasedupto2-4-foldinthetransgeneexpression.In addition, polymer PEI enhanced the transfection efficiency of the lipopeptide-mediated genedeliveryupto50-foldcomparedtocontrol.Insummary,shortfullysynthesizedlipopeptide-basedtransfectionagent facilitated transgeneexpression in severalmammaliancellsbyoptimizing theDNA-lipopeptidecomplexformationandtransfectioncondition.KeyWords:lipopeptide,particlesize,celltoxicityandtransfection.


214


CLONINGANDEXPRESSIONOFOmpCGENEFROMSalmonellaTyphiINEschrichiacoli

NadyaStepanie1,DudiHardianto1,JuwartinaIdaRoyani2,AngelinaGill2,SabarPambudi2

1CenterofTechnologyforPharmaceuticalandMedical,AgencyfortheAssessmentand

ApplicationofTechnology,LAPTIABBuilding,PuspiptekArea,TangerangSelatan,15314

2CenterforAgriculturalProductionTechnology,AgencyfortheAssessmentandApplicationofTechnology,LAPTIABBuilding,PuspiptekArea,Tangerang

Selatan,15314*Email:[email protected]

ABSTRACT

SalmonellaentericaserovarTyphi(S.Typhi) isaGram-negativebacteriumthatcausesoftyphoidfeverinhumans.Outermembraneisastructureoutsidethecytoplasmicmembraneandconsistsofphospholipids,lipopolysaccharide(LPS)andproteinsthatcoversGram-negativebacterium.Outermembrane proteins (OMPs) as porins allow the small hydrophilic molecules (<600 Da) to passthroughthechannel;actasreceptorsforbacteriophages,pathogenesisandimmunity.ThemajorporinsinSalmonellaareOmpC,OmpF,andOmpDandtheyareabundantonthesurfaceofthecell.OmpC is amajor S. Typhi porinprotein thatpotential indiagnostics and vaccinationbecauseofimmunereactivity, stability to temperatureanddenaturants,andresistant toproteolysis. In thisstudy, the OmpC of S. Typhi is cloned in pET21a(+) vector and expressed in Escherichia coliBL21(DE3). The pET21a(+) vectorwas characterized by SacI and HindIII restriction enzymes andsequencing.TheOmpCproteinwasexpressedinE.coliBL21(DE3).Keywords:SalmonellaTyphi,porins,OmpC,diagnostics,vaccination


215


SCREENINGFORACETYLCHOLINESTERASEINHIBITIONOFVEGETABLESFROMINDONESIA

MaulitaCutNuria1,3,AsepGanaSuganda1,ElinYulinahSukandar2,MuhamadInsanu1*

1DepartmentofBiologyPharmacy,SchoolofPharmacy,InstitutTeknologiBandung,Jl.Ganesha10,Bandung,40132,Indonesia

2DepartmentofPharmacology,SchoolofPharmacy,InstitutTeknologiBandung,Jl.Ganesha10,Bandung,40132,Indonesia

3DepartmentofBiologyPharmacy,FacultyofPharmacy,UniversitasWahidHasyim,Jl.MenorehTengahX/22,Semarang,50236,Indonesia


ABSTRACT

Peoplewithdementiaarefromtheelderlyandconsumevegetableseverydaycanreducetheriskofdementia.Themostcommoncaseofdementiacharacterizedbymemoryloss.Oneofthediseasescharacterized by dementia is Alzheimer’s which resulting in decreased neurotransmitteracetylcholine.Theaimofthisstudyistoinvestigatetheinhibitoryactivityofacetylcholinesteraseenzyme(AChE)fromvariousvegetableswhicharewidelyusedbySundaneseandJavanesepeopleinIndonesia.Ethanolextractsof13vegetableshavebeentestedforAChEinhibitionactivityusingEllman'scolorimetricmethodin96-wellplate.Thereare4typesofvegetablesthatcouldnotinhibittheactivityofAChE,meanwhile7typesofvegetableshadIC50valuesmorethan1000μg/mL.Only2 types of vegetables had IC50 values less than 1000 µg/mL which were ethanol extract ofO.americanumleavesandC.caudatusherb.BothofthisextractswereexpectedtohaveapromisingAChEinhibitoryactivity.

Keywords:acetylcholinesteraseinhibition,vegetables


216


HYPOGLYCEMICACTIVITYOFETHANOLICEXTRACTANDFRACTIONOFMENGKUDU(Morinda

citrifoliaL)FRUITSINGLUCOSEINDUCEDRATS

MUHTADIA1*,SUSILOWATIY2,SUMIWISA1,HARTATIS1,RAMDHININS1.,.

1DepartementPharmacologyandClinicalPharmacy,2DepartementBiologyPharmacy.FacultyofPharmacy,UniversitasPadjadjaran,Jatinangor45363,SumedangIndonesia


ABSTRACT

ToevaluatethehypoglycemicactivityofethanolicextractandfractionMengkudu(MorindacitrifoliaL.) fruits and that the fruit used empirically to treat hyperglycemia in Indonesia, in order todetermineethanolicextractandfrctionwiththemostpotentialasahypoglycemicagent.Activityhipoglycemic testwas conducted inhealtlyWistar rats, glucose inducedmale ratswithglucosetolerancetest.Theethanolicextractswiththreevariationdosesat0,3;06and1,2g/kgbwandatthesamedoses1,2/kgbwofactivityofwater,aethylacetat(base),aethylacetate(acid),andn-hexanefractionofMengkudu(MorindacitrifoliaL)fruitsgivenorally.Thebloodsampleswereusedfordeterminationofglucose levelexaminationusingspectrofotometricwithGOD-PAPmethode.TheethanolicextractofMengkudu(MorindacitrifoliaL.)fruitshasthebestactivityinreductionofbloodglucoselevelinratsatdose1,2g/kgbw,followedbydose0,6g/kgbwanddose0,3g/kgbwandalsoshowedthattheaethylacetate(acid)fractiongavethebestactivityinreductionofbloodglucoselevel inrats(54,29%),followedbywaterfraction(47,42%),aethylacetate(base)fraction(44,51%)andn-hexanefraction(34,18%).Resultsofthepresentstudysuggestethanolicextractandaethylacetate(acid)fractionatdose1,2gkg/bwofMengkudu(MorindacitrifoliaL.)fruitsarethepotentialuseintherapyofhyperglycemia.Keywords:Hypoglycemicactivity,MorindacitrifoliaL.,fruits,GlucoseToleranceTest.


217

PosterPresentation [PP-124]CHARACTERIZATIONOFNA-CMCHYDROGELSSYNTHESIZEDOFHYACINTHCELULLOSE

IdaMusfiroh*1,NadyaIndahDewanti1,AnggunPutriPerwira1,PatihulHusni2,AmiTjitraresmi3,

AhmadMuhtadi4

1DepartmentofPharmaceuticalAnalysisandMedicinalChemistry,FacultyofPharmacy,UniversitasPadjadjaran

2DepartmentofPharmaceutisandPharmaceuticalTechnology,FacultyofPharmacy,UniversitasPadjadjaran

3DepartmentofBiologicalPharmacy,FacultyofFarmacy,UniversitasPadjadjaran4DepartmentofPharmacologyandClinicalPharmacy,FacultyofPharmacy,UniversitasPadjadjaran

Email*:[email protected]

ABSTRACT

ThepurposeofthisstudywastodeterminethecharacteristicsofNa-CMCsynthesizedfromWaterHyacinthasacarrierofhydrogelbycrosslinkingusingepichlorohydrinandsuccinicacidusingsodiumdiclofenacastheactivesubstance.Themethodsofresearchincludedwereα-celluloseisolation,Na-CMCsynthesiswithalkalinationandcarboxymethylationprocesses,optimizationofsynthesizedNa-CMC concentration as a base of hydrogel, and determine swelling behavior of hydrogel,characterizationof functionalgroupsbyFTIR (FourierTransform InfraredSpectroscopy)andPSA(ParticleSizeAnalyzer);thendiffusionprofiletestofsodiumdiclofenacinNa-CMChydrogelbyFranzmethod. The results showed that the characterization Na-CMC synthesis hydrogel from waterhyacinthwithepichlorohydrin,succinicacidandstandardofNaCMCshowedswellingratiovaluesof54%,1000,2%and1218.75%.AbsorptionofC=Ogroupsat1631.78cm-1 ;1716,65cm-1and1604.77cm-1 ;1419,61cm-1.Aparticlesizeof Na-CMCsynthesishydrogelwithepichlorohydrin,succinic acid, and standardwere1.212nm,1.338nmand1.197nm respectively. The results ofdiffusion test of sodium diclofenac in the synthesis Na-CMC hydrogel fromwater hyacinth andstandardwere13.01%,0,6714%and8.56%, respectively.The resultsofdiffusionprofile showedthatNa-CMC synthesized bywater hyacinth crosslinking using epichlorohydrin and succinic acidimprovedthediffusionprofile.CharacteristicsofsynthesisNa-CMCfromwaterhyacinthcelluloseusingawiththeadditionofcrosslinkerephlorohydrinandsuccinicacidashydrogelwhichhasbettercharacteristics than Na-CMC synthesis without crosslinker. However with epichlorohydrin, itimprovesparticlesizebutnotforswellingratio.DiffusionprofilewithsodiumdiclofenacasanactivesubstancewhichhavebyNa-CMC synthesiswithepichlorohydrinand succinic acid crosslinker isbetterthanNa-CMCsynthesiswithoutcrosslinker.Keywords:Synthesized,Waterhyacinth,DiffusionProfile,Na-CMC,epiclorohydrin,succinicacid


218

PosterPresentation [PP-125]QUANTITATIVESTRUCTURE-ACTIVITYRELATIONSHIPANDMOLECULARDOCKINGSTUDY

OFSEVERALFLAVONOIDSASCOX-2INHIBITORS

DadanSuryasaputra*,RinaAnugrah,AlgiIkhsan,Rismaya

FakultasFarmasi,UniversitasJenderalAchmadYani,Jln.TerusanJend.SoedirmanPO.BOX148,Cimahi,WestJava,Indonesia


ABSTRACTSelectiveCOX-2inhibitorcompoundsshowanti-inflammatory,antipyreticandanalgesiceffectsinexperimental animals and humans. Flavonoids are polyphenol group compounds that had anti-inflammatoryactivityandhasbeenconsideredanewclassofnaturalanti-inflammatoryagentsbyinhibitingCOX-2enzyme,includinghesperidin,diosmin,andginkgetin1.Thisstudyobjectivewastodetermine the interaction of several flavonoids (luteolin, kaemferol, apigenin, quercetin,quersitrine,catechin,baikalein,rutin,andmirisetin)withtheCOX-2enzymeanditspotentialasaCOX-2selective inhibitorusingQuantitativeStructure-ActivityRelationship(QSAR)andmoleculardockingapproach.TheQSARequationmodelwasbuiltby19derivativesofcelecoxibcompoundswith seven descriptors,while formolecular docking using 3 enzyme structure; 6COX, 4COX and3PGHas targetprotein. Structurepreparation,geometryoptimizationandpredictor calculationswereperformedusingMarvinsketch®v15.12andDiscoveryStudio®client2017,forvalidationandstatistical calculations using EasyQSAR® v1.0. The molecular docking study used Autodoc Vina®v1.5.6,wheredockingvalueswerecalculatedusingtheGibb’sfreeenergy(ΔG).TheselectedQSARequationmodelwas:LOG(1/IC50)=5.537–0.682*(LogP)-5.275x10-9*(HOMO)-7.332x10-2*(LUMO)-7.04x10-3*(Molar_Refractivity)+0.321*(Randix_Index)-2.0189x10-2*(Harary_Index)-3.978x10-3*(Moment_Dipole),withstatisticalanalysis:r2=0.974SE=0.04Fcalc/Ftab=16.11.ThecompoundspredictedtohadthebestCOX-2 inhibitorsactivitywererutin,quersitrin,mirisetinandquersetinwithalog(1/IC50predicted)valueof7.52μM;6.25μM;5.48μM;And5.46μMrespectively.Basedon the results of docking study, 4 potential flavonoids obtained as COX-2 inhibitors :mirisetin,luteolin,catechin,andapigenin,withRMSD0,2301±0,0185andΔGvaluesrespectively-8.57;-8.44;-8.68; and -8.54 (kcal/mol). These four potential compounds interact with the glucocorticoidreceptorstoformhydrogen,hydrophobic,andVanderWaal'sbondsbyinvolvingtheaminoacidsHis90,Ser353,Gln192,Ser530,Val349,andPhe518fromthereceptor.Keywords:QSAR,MolecularDocking,COX-2Inhibitor,Flavonoids.


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ANTIDIABETICSTANDARDMEDICINESOFPISANGRANGGAP(MUSAPARADISIACA)WHICHGROWSATTHEFOOTOFMOUNTGALUNGGUNG,TASIKMALAYA

DolihGozali1,DanniRamdhani2,ResmiMustarichie2*

1PharmaceuticalDepartment,FacultyofPharmacy,UniversitasPadjadjaran,Indonesia453632PharmaceuticalAnalysisandMedicinalChemistry,FacultyofPharmacy,Universitas

Padjadjaran,Indonesia45363*Email:[email protected]

ABSTRACT

Diabetesmellitusisachronicdisorderespeciallyinrelationtocarbohydratemetabolisminthebodythat characterizedbyhyperglycemia. Traditionally, pisang ranggap (Musaparadisiaca) is usedasDiabetesmellitustreatmentintheareaofthefootofMountGalunggung,Tasikmalaya.Thisresearchwas conducted toverify scientificallyof this issue.Theantidiabeticactivityofethanolextractofpisangranggapwastestedinvivoinmaleratswithalloxaninductionmethod.Theresultshowedthattherewas500mg/kgbodyhasthebesteffectondecreasingbloodglucoseleveldiabeticrats.Keywords:DiabetesMellitus,Antidiabetic,pisangranggap,Musaparadisiaca,Alloxan


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PosterPresentation [PP-127]ENHANCINGINTRACELLULARTRANSPORTOFRIFAMPICINUSINGACEMANNANMODIFIEDLIPID

NANOPARTICLES

TriSuciati1,MuhammadLuthfiNugraha1,RismawatiLailaQodariah1,YaniTriyani2,MarliaSinggihWibowo1

1SchoolofPharmacy,BandungInstituteofTechnology,Jl.GaneshaNo.10,Bandung40132,Indonesia2FacultyofMedicine,BandungIslamicUniversity,Jl.TamansariNo.2,Bandung40116,

Indonesia


ABSTRACT

Poor cellular transport of antibiotics hinder eradication of intracellular pathogens1. Here, weproposed the use of acemannan—an acetylated polymannose of aloe gel isolate—to modifynanostructuredlipidcarrier(NLC)fordeliveringrifampicinintracellularly.Tofacilitateacemannanbindingon theNLC surface, itwasconjugatedwithchitosan (CS-ACE)using sodiumborohydrideaminereduction.TheCS-ACEconjugateshowedawavelengthnumberchangeofamidebandandSchiffbaseformationontheFTIRspectra.Rifampicinwasdissolvedinoil-lipidblendandemulsifiedusingpolysorbate80toformNLCnanoparticles.Theconjugatewasaddedinthewaterphasepriorto emulsification and particle solidification was conducted by ionotropic gelation-solventevaporation.Ahomogenouscompositenanoparticleswasproducedatthesizeoflessthan500nmwith high encapsulation efficiency and drug loading of rifampicin. Intracellular penetration ofnanolipid formulation containing nile red on Vero cells was shown using confocal microscopeimaging.TheCS-ACEnanolipidenhancedintracellularaccumulationcomparedtoun-modifiedandchitosanmodifiedNLCthatindicatesthesuccessofintracellulartargeting.

Keywords:acemannan,nanostructuredlipidcarrier,intracellulartargeting,rifampicin,Verocells


221


ACTIVITYOFREDALGAE(EUCHEUMACOTTONII)AGAINSTSOMEBACTERIAANDFUNGI

Sulistiyaningsih*,YoppiIskandar,andEliHalimah

FakultasFarmasiUniversitasPadjadjaranJalanRayaBandung-SumedangKM21,5JatinangorSumedang45363


ABSTRACT

InfectiousdiseasesareasignificantproblemandcontributetosignificantmorbidityandmortalityratesincludingIndonesia[1].Redalgae(Eucheumacottonii)produceavarietyofbioactivesecondarymetabolitesasantimicrobial,anthelmintic,andcytotoxicsubstances,soithaspotentialasasourceofbioactivesecondarymetabolitessuchasflavonoidsthathaveantibacterialactivity[2,3].Thisresearchaimstoknowthepotentialextractandfractionsoftheredalgaeasnaturalantimicrobe.Thesamplewasextractedbymacerationusingethanol96%andfractionatedbyliquid-liquidextractionusingn-hexane,ethylacetate,andwater.TheantimicrobialtestwasdonebyagardiffusionandmicrodilutionagainstbacteriaStaphylococcusaureus,Methicillin-ResistantStaphylococcusaureus(MRSA),Pseudomonasaeruginosa,andPseudomonasaeruginosaMethicillin-Resistant(PAMR);andfungiCandidaalbicansandAspergillusniger.TheresultsshowedthatE.cottoniiextracthavenotanyinhibitoryzones,butthefractionsshowedinhibitoryzonesforsomebacteria.EthylacetatefractionisactiveagainstbacteriaS.aureusandP.aeruginosa;waterfractionsisactiveagainstS.aureus,MRSAandPAMR.However,n-hexanefractionwasnotactiveeitheragainstmicrobesorfungi.SoE.cottoniihasthepotentialactivityandcanbedevelopedasanaturalantimicrobialKeywords:RedAlgae,Eucheumacottonii,naturalantimicrobial


222

ACKNOWLEDGMENT

TheorganizingcommitteegreatlyappreciatetothefollowingwhohavesupportedandcontributedtothisSeminar:● Keynotespeakers

- DirectorGeneralofPharmacyandMedicalDevices(Indonesia)- Prof.HidetoshiArima(Japan)- Assoc.Prof.VeyselKayser(Australia)

● Invitedspeakers:Prof.Dr.Dato’IbrahimJantan(Malaysia),Prof.Dr.MohdCairulIqbalMohdAmin(Malaysia),Prof.Dr.YahdianaHarahap,M.S.,Apt. (Indonesia),Prof.Muchtaridi,Ph.D.,Apt. (Indonesia), Assoc. Prof. Nurul Asma Abdullah, Ph.D. (Malaysia), Dr. Nur KUsaira bintiKhairulIkram(Malaysia),Dr.rer.nat.AnisYohanaChaerunisaa,Apt.(Indonesia)

● Oralandposterpresenters● AllParticipants● 3rdISPST-2018Committee

Sponsors1.PT.CendoPharmaceuticalIndustries2.BankRakyatIndonesia3.BankBJB4.JournalofYoungPharmacy(JYP)5.JournalofPharmaceuticalSciencesandResearch(JPSR)6.DrugInventionToday(DIT)7.IndonesianJournalClinicalPharmacy(IJCP)8.IndonesianJournalofPharmaceuticalSciencesandTechnology(IJPST)9.AsianFederationofPharmaceuticalScience

rd international seminar on pharmaceutical science and...

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