raman investigation of temperature profiles of phospholipid dispersions in the biochemistry...
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Nicolet 6700 FT-IR Spectrometer and Associated NXR Raman ModuleTRANSCRIPT
RAMAN INVESTIGATION OF TEMPERATURE PROFILES OF PHOSPHOLIPID DISPERSIONS IN
THE BIOCHEMISTRY LABORATORY
NORMAN C. CRAIG, Department of Chemistry and Biochemistry, Oberlin College, Oberlin, OH USA
Nicolet 6700 FT-IR Spectrometer and Associated NXR Raman Module
Variable Temperature Cell
Top View of Sample Compartment in Raman Module
Raman Spectrum of DPPC at ca 15°C
Spectrum Parameters
Excitation: Nd:YVO4 laser at 1064 nm, ca 0.5 WAnalysis and detection: 180° optics, FT-IR, InGaAsConditions: 8 cm-1 resolution, 200 scans (3 min) Sampling: 1.8 mm m. p. capillary (borosilicate glass); 50% phospholipid in D2O; centrifuged warm. Tiny sample size enables the experiment.
Other Raman spectrometers: simpler, dispersive instruments with CCD detection and excitation with red laser light.
Raman Spectra of CH-Stretching Region at Two Temperatures
Temp 15°C Temp ca 65°C
Temperature Profiles for Various Phospholipids
Acknowledgments
• NSF support for the instrument.• Nanette Lacuesta, as a college senior, assisted
in developing the experiment.• Professor William H. Fuchsman first
introduced the experiment in the laboratory of his biochemistry course.