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XT-2000iV/XT-1800iV Output Format to Host Computer (Version 00-08 or higher) 1/32 Software of Automated Hematology Analyzer for Animal XT-2000iV/XT-1800iV Output Format to Host Computer Ver.00-08 or higher Released on February 01, 2006 SYSMEX CORPORATION

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XT-2000iV/XT-1800iV Output Format to Host Computer (Version 00-08 or higher) 1/32

Software of Automated Hematology Analyzer for

Animal

XT-2000iV/XT-1800iV Output Format to Host Computer

Ver.00-08 or higher

Released on February 01, 2006

SYSMEX CORPORATION

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Contents

1. Scope of application........................................................................................................................ 3

2. Revision history............................................................................................................................... 3

3. Output Format to Host Computer ................................................................................................ 5 3.1. Serial Communication ......................................................................................... 5 3.2. TCP/IP Communication ....................................................................................... 7 3.3. Data Format ........................................................................................................ 8 3.4. Analysis Data Format .......................................................................................... 8

3.4.1. Analysis Data Format 1 ............................................................................... 13 3.4.2. Analysis Data Format 2 ............................................................................... 15 3.4.3. Analysis Data Format 3 ............................................................................... 16 3.4.4. Analysis Data Format 4 ............................................................................... 17 3.4.5. Analysis Data Format 5 ............................................................................... 18 3.4.6. Analysis Data Format 6 ............................................................................... 18

3.5. QC Data Format ................................................................................................ 20 3.5.1. QC Data Format 1 ....................................................................................... 20 3.5.2. QC Data Format 2 ....................................................................................... 21 3.5.3. File Corresponding to QC No. ..................................................................... 22

4. Communication Specifications of Analysis Information with Host Computer ....................... 23 4.1. Outline .............................................................................................................. 23 4.2. Serial Communication ....................................................................................... 23 4.3. TCP/IP Communication ..................................................................................... 26 4.4. Text Format ...................................................................................................... 27 4.5. Analysis Information Inquiry Format ................................................................. 28 4.6. Analysis Information Format ............................................................................. 29

4.6.1. Analysis Information Format 1 .................................................................... 29 4.6.2. Analysis Information Format 2 .................................................................... 30

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1. Scope of application This document describe about host format for XT-2000iV/XT-1800iV.

2. Revision history This document describe about host format for XT-2000iV/XT-1800iV. Edition Revision date Revision contents

First Initial version Second 2004/06/09 Output PLT-I and PLT-O,

Added “Animal Species” and “Category” to Analysis information format.

The 3rd 2004/11/01 Change RET# field - 5digits to 6digits (Analysis data format3’ -size of format 253 bytes to 254 bytes)

The 4th 2005/3/9 1. Revise the code of animal species <wrong> <correct>

Mouse “003” ‘001” Rat “002” “002” Rabbit “005” “003” Dog “001” “004” Monkey “005” “005” Other “000” “007” Other1 --- “008” Other2 --- “009” Other3 --- “010” Other4 --- “011” Other5 --- “012”

2. Addition of category’s description Rat standard “001” Mouse standard “002” Monkey standard “003” Dog standard “004” Rabbit standard “005” Other1 standard “006” Other2 standard “007” Other3 standard “008” Other4 standard “009” Other5 standard “010” Other standard “129”

The 5th 2005/5/30 1. Title of documents Output documents -> Output format to host computer

2. Added explanation for format 3. Revise “Nickname” to “Name” for Instrument’s Name

Analysis data format 2-6, QC format 1,2 The 6th 2005/06/17 1. Revise Analysis data format. 1

Change Example of “Instrument's Name” to "_XT-2000iV^11001". Added Remarks “0 padding” for “Sample No.”. Added “Monocytosis”. Change “Reserved” field – 7digits to 6digits.(after “Leukocytopenia”) Change Last “Reserved” filed – 31digits to 16digits. Change in the Example of “Instrument's Name” – “200V” to “2000”. 2. Revise Analysis data format 2 Added Remarks “0 padding” for “Sample No.”. Change “Age/LL” field – 3digits to 5digits with decimal point. Change “Age/UL” field – 3digits to 5digits with decimal point. Revise “ManualAna.Result(W)” to “ManualAna.Result(R)”. Revise “ManualAna.Result(R)” to “ManualAna.Result(P)”. Revise “ManualAna.Result(P)” to “ManualAna.Result(W)”. Moved “ManualAna.Profile” to after “Extend Ana. Result(R)”.

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Change “Reserved” field – 116digits to 112digits. 3 Revise Analysis data format 3 Added Remarks “0 padding” for “Sample No.”. Revise Remarks for “PLT-I: ”XXXXF” to “XXXX”0””. Revise Remarks for “PLT-O: ”XXXXF” to “XXXX”0””. Change in the Example of “Instrument's Name” – “200V” to “2000”. 4 Revise Analysis data format 4 Added Remarks “0 padding” for “Sample No.”. Deleted “3” and “4” from “DIFF-0#” Example. 5 Revise Analysis data format 5 Added Remarks “0 padding” for “Sample No.”. Deleted “3” and “4” from “RET-0#” Example. Change the Length of “RET-0#” to “RET-8#” field to 10digits. Change the Length of “RET-1%” to “RET-8%” field to 7digits. Change “Reserved” filed – 5digits to 95digits. Change “Total” filed – 163digits to 288digits. Change Remarks for “RET-*”. 6 Revise Analysis data format 6 Added Remarks “0 padding” for “Sample No.”. Change “Reserved” filed – 60digits to 206digits. Change “Total” filed – 161digits to 307digits. 7. Revise some expressions for Analysis Data Format. 8. Revise QC Data Format 1 and QC Data Format 2 Change in the Example of “Instrument's Name” – “200V” to “2000”.

The 7th Unused number to synchronize with software version The 8th

306A007 2006/01/25 This document was first registered as formal technical drawing.

1. “PLT information” for PLT-I/PLT-O selected as reportable “PLT” 2. Addition « PLT-O » to “Analysis information Format1” 3. Addition Animal species and categories for Horse, Cat and Guinea pig “013“:Horse, “014“:Cat, “015“:Guinea pig “130“:Horse std, “131“:Cat std, “132“:Guinea pig std

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3. Output Format to Host Computer The bit serial voltage type, which conforms to the RS-232C interface, and TCP/IP protocol are used for host computer output of XT-2000iV/XT-1800iV. The serial interface port and LAN port for the connection with the host computer is on the rear panel of the IPU.

3.1. Serial Communication Serial port is used for sending analysis data and QC data.

1) Connector

- Connect to the host computer output connector on the rear panel of the IPU.

- Use a 9-pin, D-SUB female connector.

- Pitch of connector fixing screws adopts the size in inch.

2) Connector signal

Pin No. Signal Name Signal Direction

1 2 Receive Data (RxD) To XT-2000iV/XT-1800iVfrom HOST 3 Transmit Data (TxD) From XT-2000iV/XT-1800iVto HOST 4 Data Terminal

Ready (DTR) From XT-2000iV/XT-1800iVto HOST

5 Signal Ground (SG) 6 Data Set Ready (DSR) To XT-2000iV/XT-1800iVfrom HOST 7 Request to Send (RTS) From XT-2000iV/XT-1800iVto HOST 8 Clear to Send (CTS) To XT-2000iV/XT-1800iVfrom HOST 9

3) Communication Setting

Communication setting is start-stop asynchronous half duplex (asterisk indicates setting at shipment from factory), and adjustment can be made as follows.

Baud Rate 600, 1200, 2400, 4800, *9600, 14400, 19200, 38400 (BPS) Code 7-bit, *8-bit Stop Bit *1-bit, 2-bit Parity Bit *None, Even, Odd Class Class A, *Class B Interval 0, 1, *2, 3, 5, 7, 10, 15 (seconds)

4) Signal Level

Signal level of the RS-232C conforms to EIA RS-232C V.24. Level Data Signal Control Signal

+3V or Higher Logic "0," Start Bit ON -3V or Lower Logic "1," Stop Bit OFF

5) Communication Format

The format is start-stop asynchronous half duplex (asterisk indicates setting at shipment from factory), and adjustment can be made as follows.

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(1) Code ASCII codes are used for all the output to the computer.

(2) Text Format “STX”(02h) is sent at the beginning, and “ETX”(03h) is sent at the end of data.

(3) Transmission Procedure Two classes are prepared and can be selected depending on the system status. (Class B is selected at the shipment from factory.)

- Class A One-way data transmission which requires no response from the computer.

- Class B Two-way communication which requires response (ACK, NAK) from the computer.

[XT-2000iV/XT-1800iV] [Computer]

Sends data (1 text)

Sends ACK (06H) if there is no error, or NAK (15H) if there is an error

In case of ACK, sends next text. In case of NAK, resends (up to 3 times)

(4) Transmission Error

If an error is detected, the transmission is interrupted and the error message is displayed on the IPU. The operator must recover the error. Cases of errors are as follows.

Error name Descriptions IPU Action to be taken

Transmission Error Detects Frame Error, Parity Error or Over-run Error, when receiving.

Interrupt

Off-line HC’s control signal “DSR” is OFF. Interrupt ACK Time-out After data transmission, no response was received from HC

in 15 seconds. (Class B only) Interrupt

(No error) After data transmission, NAK was received from HC. (Class B only)

Re-send

(No error) After data transmission, a response other than ACK and NAK was received from HC. (Class B only)

Re-send

No. of re-send over After data transmission, the 4th response other than ACK was received from HC. (Class B only)

Interrupt

Transmission Time-out

When sending, control signal CTS does not become active in 5 seconds. (only when RTS/CTS control is activated)

Interrupt

STX Time-out (1) After HC’s requested to send, STX was not received from HC in 15 seconds. (Class A only)

(2) After HC’s requested to send, STX was not received from HC 15 seconds after ACK was received. (Class B only)

(3) Within 15 seconds after receiving the sub-text in the previous text, STX of the following sub-text was not received. (Class A only)

(4) Within 15 seconds upon sending ACK after receiving the sub-text in the previous text, STX of the following sub-text was not received. (Class B only)

Interrupt

ETX Time-out ETX was not received within 15 seconds after receiving STX. Interrupt

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(5) Transmission Timing Selection between the transmission for every analysis cycle and the batch transmission of stored data is possible by the IPU settings. The data transmission interval can be set (from zero second) also.

(6) Transmission Interval The transmission interval of sample data can be set on the IPU. The interval is a time

period after the ACK/NAK response until the next data transmission in the case of class B.

3.2. TCP/IP Communication The network interface conforms to IEEE802.3, is used for host computer output of XT-2000iV/XT-1800iV. The network port for the connection with the host computer is on the rear panel of the IPU.

1) Network interface layer

The network interface conforms to IEEE802.3. Communication is performed by 10Base-T. The RJ45 socket is used as a hub for XT-2000iV/XT-1800iV connection The cable of UTP category 5 is used for communication.

2) TCP/IP

• Before communication, the text format including STX and ETX is set to the TCP's data unit.

• The IP address of XT-2000iV/XT-1800iV's Main unit is set (The default value is 192.168.28.142 and this value may be changed on Windows networking setting and Main unit by service engineer.)

• The IP address for XT-2000iV/XT-1800iV's host communication is set. ( This value may be changed on IPU setting screen and we have to use other IP address without 192.168.28.* )

• The TCP port number for XT-2000iV/XT-1800iV's host communication is set. (The default value is 5000 and this value may be changed on IPU setting screen.)

3) TCP Connection

The host computer is defined as server, and the XT-2000iV/XT-1800iV as client for connection. The XT-2000iV/XT-1800iV checks the connection when the system starts. When the connection fails, the XT-2000iV/XT-1800iV repeats the connection at regular intervals. If the server shuts down after the connection, the XT-2000iV/XT-1800iV does not perform the connection again.

4) Application Layer

Communication is performed according to the bit serial interface class A.

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5) Text and Communication Protocol (1) Communication Control Character

ASCII codes are used for all the output to the computer. (2) Text Format

“STX” is sent all the beginning, and “ETX” is sent at the end of data.

6) Communication Error Processing When a communication error occurs, the communication is stopped and the error is displayed on the IPU. The restoration of communication can be made by the operator. A communication error occurs in the case mentioned below. • No response is made from the computer within 30 seconds after the data transmission.

7) Communication Timing

Depending on the settings of the IPU, it is possible to select between communication per analysis cycle and communication of the stored data in a batch.

3.3. Data Format The output to host computer consists of sample data and QC data, and they differ in length and content of the text. (They are classified by the sample class codes.) • Output of analysis results is the text data only, and the text class I is always "D." • The text distinction II is usually "1." But when the text length is 255 bytes or more, the text is divided into two or more for transmission, and the text block order is expressed by the numeral of the text distinction II. (ETB code is not used.) • Sample distinction code is "U" in the case of sample data, and is "C" in the case of QC data. • The QC data is output in the QC format. This data can be output in the QC menu of the IPU.

3.4. Analysis Data Format 1) Output Order

The order of transmission is from the top parameter to the bottom. The data sent is the most significant digit first. Zero-suppression is not carried out.

2) Decimal Point Decimal point is not sent. Therefore, it is necessary to add decimal point specified for each item at the host computer.

3) Date The order of Year/Month/Day is fixed. Zero-suppression is not carried out.

4) Rack No. This is the number assigned to a sample rack, and consists of 6-digit number. Zero-suppression is not carried out. (This field is filled with space code if not used.)

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5) Tube Position This indicates the analysis position of aimed sample in a sample rack, and consists of number from 1 to 10. Zero-suppression is not carried out.

6) Sequence No. This indicates the sequence number of the sample analyzed on the same day, and consists of 10-digit number. Zero-suppression is not carried out.

7) Sample ID Number This number consists of 15 digits in numeral or alphabet which may include a hyphen "-" (2HD) between numerals as needed. A hyphen "-" have to be included in 15 digits. Zero suppression is not carried out. Zero paddings are applied.

8) Construction and Flag of Numerical Value The numerical value is constructed as follows. Zero suppression is not carried out. For the RESERVED parameters, "0" is output.

Most significant Digit -> Least Significant Digit

X X X X F Data Flag

Details of Flag 0: Normal 1: Analysis data is greater than the preset Upper Patient

Mark Limit. 2: Analysis data is less than the preset Lower Mark Limit. 3: Out of linearity limit. 4: Analysis data is less reliable

9) Abnormal Value Data

When the value data is displayed with "----," the data is output in the form of "*0000".

10) Instrument Name This is the information to identify each instrument, and consists of alpha-numeric (capital). The one for 16-character of the head is outputted. The space is put at the beginning when the number of the characters is under 16-character.

11) Analysis Information

This is to display the analysis status of a sample. 0: Normal analysis 1: Abnormal analysis

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12) Sample Judgment Information(NEG/POS/ERR/QC Information) This is to display the judgment information of aimed sample whether re-analysis is necessary. 0: Negative 1: Positive only 2: Error only 3: Positive+Error Q: QC data

13) Order Information

This indicates the analysis order information for each analysis parameter. "0": Not analyze "1": Analyze

14) Sample Information (Flag)

The existence of the IP message of WBC, RBC or PLT is indicated. X X X X X X 1 2 3 4 5 6 1. WBC Abnormal 2. WBC Suspect (Reserved) 3. RBC Abnormal 4. RBC Suspect (Reserved) 5. PLT Abnormal 6. PLT Suspect (Reserved) 0: None 1: Existing

15) Sample ID Information

This displays the implication of the sample number. 4: Sample number was read by the ID bar code reader 2: Sample number failed in reading by the ID bar code reader 0: Other

16) Analysis Mode This is to show the one of following. 1: Manual analysis 2: Sampler analysis 3: Closed analysis 4: Capillary analysis

17) Patient ID

This field is reserved in XT-2000iV/XT-1800iV.

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18) Positive (Diff) When a blood cell differentiation value is abnormal, output "1," if not, output "0."

19) Positive (Morph)

When a blood cell morphology is abnormal, output "1," if not, output "0."

20) Positive (Count) When a blood cell count value is abnormal, output "1," if not, output "0."

21) Error (Func)

When an analysis error other than the ID bar code read error has occurred, output "1," if not, output "0."

22) Error (Result)

When a sample-related error such as "Sample Not ASP.," "Short Sample," or "Low Count error" has been occurred, output "1," if not, output "0."

23) The Unit Information

When the Dutch SI unit is being used, output "1," if others, output "0."

24) PLT Information When PLT-O is adopted as a PLT value, output "1," if others, output "0." In case of XT-1800iV, output “0”.

25) Instrument ID

A manufacturer has an instrument ID composed of 22 fixed characters with the information to identify the instrument.

26) Analysis Date (Ana. Year, Ana. Month, Ana. Day)

This date is for reanalyzed date with analysis profile.

27) Analysis Time (Ana. Hour, Ana. Minute) This time is for reanalyzed time with analysis profile.

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28) Animal species This is ID for measured animal species. ”001”: Mouse ”002”: Rat ”003”: Rabbit ”004”: Dog ”005”: Monkey ”007”: Other ”008” Other1 ”009”: Other2 ”010”: Other3 ”011”: Other4 ”012”: Other5 “013”: Horse “014”: Cat “015”: Guinea pig

29) Category

This is ID for measured category. ”001”: Rat std ”002”: Mouse std ”003”: Monkey std ”004”: Dog std ”005”: Rabbit std ”006”: Other1 std ”007”: Other2 std ”008”: Other3 std ”009”: Other4 std ”010”: Other5 std ”129”: Other std “130”: Horse std “131”: Cat std “132”: Guinea pig std

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3.4.1. Analysis Data Format 1 ITEM LENGTH EXAMPLE REMARKS Text Distinction Code1 1 'D'(fixed) Text Distinction Code2 1 '1'(fixed) Sample Distinction Code 1 'U'(fixed) Instrument's Name 16 "_XT-2000iV^11001" Right Align Sequential Number 10 Sequential number created each

analyzing Reserved 3 "000"(fixed) All letters are "0" Sample No. 15 "000012345678901" Right align with leading 0

padding. Year 4 "2002" 4 figures year in A.D. Month 2 "07" 2 figures month in A.D. Day 2 "04" 2 figures day in A.D. Hour 2 "18" 2 figures hour in 24 hour format Minute 2 "09" 2 figures minutes in 24 hour

format Reserved 2 "00"(fixed) Rack Number 6 "001234" 0 padding. “ “ (if not used)Position of Tube 2 "08" 0 padding Sample ID Information 1 "0": Barcode Reader is not used

"2": Barcode Reading was failure "4": Barcode Reading was success

Analyzing Mode 1 ''1': manual. '2': sampler. '3': closed. '4': capillary

Patient ID No. 16 “0000000000000000”(fixed) Analysis Information 1 "0":Analysis error did not occur.

"1":Analysis error occurred.

NEG/POS/ERR/QC Information

1 "0":Negative "1":Positive "2":Error "3":Positive+Error "Q":QC data.

Positive(Diff.) 1 "0": not Diff. Positive "1":Diff. Positive Positive(Morph.) 1 "0": not Morph. Positive

"1":Morph. Positive

Positive(Count) 1 "0": not Count Positive "1":Count Positive

Error (Func.) 1 "0": not Func. Error "1":Func. Error Error (Result) 1 "0": not Result Error "1":Result Error Order Information 1 "0": The order information did not exist.

"1": The order information existed. “2”: Host Order

WBC Abnormal 1 "0":No Flag "1":Flagged WBC Suspect 1 "0"(fixed) RBC Abnormal 1 "0":No Flag "1":Flagged RBC Suspect 1 "0"(fixed) PLT Abnormal 1 "0":No Flag "1":Flagged PLT Suspect 1 "0"(fixed) Unit Information 1 "0":non SI. "1":SI Reserved 1 "0"(fixed) PLT Information 1 "0":PLT-I "1":PLT-O WBC Abn Scattergram 1 "0" Not flagged "1" Flagged WBC Abnormal Flags Neutropenia 1 "0" Not flagged "1" Flagged WBC Abnormal Flags Neutrophilia 1 "0" Not flagged "1" Flagged WBC Abnormal Flags Lymphopenia 1 "0" Not flagged "1" Flagged WBC Abnormal Flags Lymphcytosis 1 "0" Not flagged "1" Flagged WBC Abnormal Flags Leukocytosis 1 "0" Not flagged "1" Flagged WBC Abnormal Flags Monocytosis 1 "0" Not flagged "1" Flagged WBC Abnormal Flags Eosinophilia 1 "0" Not flagged "1" Flagged WBC Abnormal Flags Basophilia 1 "0" Not flagged "1" Flagged WBC Abnormal Flags Leukocytopenia 1 "0" Not flagged "1" Flagged WBC Abnormal Flags Reserved 6 "0"(fixed) WBC Abnormal Flags Blast ? 1 "0"(fixed) WBC Suspect Flags Immature Gran ? 1 "0"(fixed) WBC Suspect Flags Left Shift ? 1 "0"(fixed) WBC Suspect Flags Reserved 1 "0"(fixed) WBC Suspect Flags NRBC ? 1 "0"(fixed) WBC Suspect Flags

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Reserved 1 "0"(fixed) WBC Suspect Flags Reserved 1 "0"(fixed) WBC Suspect Flags Atypical Lympho ? 1 "0"(fixed) WBC Suspect Flags RBC Lyse resistance ? 1 "0"(fixed) WBC Suspect Flags Abn Lympho/ Blasts ? 1 "0"(fixed) WBC Suspect Flags Reserved 6 "0"(fixed) WBC Suspect Flags RBC Abn Distribution 1 "0" Not flagged "1" Flagged RBC Abnormal Flags Dimorphic Population 1 "0" Not flagged "1" Flagged RBC Abnormal Flags Anisocytosis 1 "0" Not flagged "1" Flagged RBC Abnormal Flags Microcytosis 1 "0" Not flagged "1" Flagged RBC Abnormal Flags Macrocytosis 1 "0" Not flagged "1" Flagged RBC Abnormal Flags Hypochromia 1 "0" Not flagged "1" Flagged RBC Abnormal Flags Anemia 1 "0" Not flagged "1" Flagged RBC Abnormal Flags Erythrocytosis 1 "0" Not flagged "1" Flagged RBC Abnormal Flags RET Abn Scattergram 1 "0" Not flagged "1" Flagged RBC Abnormal Flags Reticulocytosis 1 "0" Not flagged "1" Flagged RBC Abnormal Flags Reserved 6 "0"(fixed) RBC Abnormal Flags RBC Agglutination ? 1 "0"(fixed) RBC Suspect Flags Turbidity/HGB Interf ? 1 "0"(fixed) RBC Suspect Flags Iron Deficiency ? 1 "0"(fixed) RBC Suspect Flags HGB Defect ? 1 "0"(fixed) RBC Suspect Flags Reserved 1 "0"(fixed) RBC Suspect Flags Fragments ? 1 "0"(fixed) RBC Suspect Flags Reserved 10 "0"(fixed) RBC Suspect Flags PLT Abn Distribution 1 "0" Not flagged "1" Flagged PLT Abnormal Flags Thrombocytopenia 1 "0" Not flagged "1" Flagged PLT Abnormal Flags Thrombocytosis 1 "0" Not flagged "1" Flagged PLT Abnormal Flags PLT Abn Scattergram 1 "0" Not flagged "1" Flagged PLT Abnormal Flags Reserved 12 "0"(fixed) PLT Abnormal Flags Reserved 2 "0"(fixed) PLT Suspect Flags PLT Clumps ? 1 "0"(fixed) PLT Suspect Flags Reserved 1 "0"(fixed) PLT Suspect Flags PLT Clumps(S) 1 "0"(fixed) PLT Suspect Flags Reserved 11 "0"(fixed) PLT Suspect Flags Ana. Year 4 "2002" 4 figures year in A.D. Ana. Month 2 "07" 2 figures month in A.D. Ana. Day 2 "04" 2 figures day in A.D. Ana. Hour 2 "18" 2 figures hour in 24 hour format Ana. Minute 2 "09" 2 figures minutes in 24 hour

format Animal Species 3 “001”:Mouse “002”:Rat “003”:Rabbit

“004”:Dog “005”:Monkey “007”:Other “008”Other1 “009”:Other2 “010”:Other3 “011”:Other4 “012”:Other5 “013“:Horse “014“:Cat “015“:Guinea pig

“000” to “127”

Reserved 16 "00…00"(fixed) All letters are "0" Instrument ID(fixed) 22 "XT-2000^12345678^11001” Name of the instrument '^' PS

code '^' Serial number Total(without STX,ETX) 253

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3.4.2. Analysis Data Format 2 ITEM LENGTH EXAMPLE REMARKS Text Distinction Code1 1 "D"(fixed) Text Distinction Code2 1 "2"(fixed) Sample Distinction Code 1 "U"(fixed) Instrument's Name 16 Sequence No. 10 "0012345678" Reserved 3 "000"(fixed) Sample No. 15 "000012345678901" Right align with leading 0

padding. Sample Category ID 3 “001”:Rat std “002”:Mouse std

“003”:Monkey std “004”:Dog std “005”:Rabbit std “006”:Other1 std “007”:Other2 std “008”:Other3 std “009”:Other4 std “010”:Other5 std “129”:Other std “130“:Horse std “131“:Cat std “132“:Guinea pig std

"000" to "127"

Sample Category 15 Right align Animal Species 3 “001”:Mouse “002”:Rat “003”:Rabbit

“004”:Dog “005”:Monkey “007”:Other “008”Other1 “009”:Other2 “010”:Other3 “011”:Other4 “012”:Other5 “013“:Horse “014“:Cat “015“:Guinea pig

"000" to "127"

Sex 1 “0”: Both "1": Male "2":Female Age/LL 5 "000.0" to "999.9" Age/LL Unit 1 "1":Day "2":Month "3":Year Age/UL 5 "000.0" to "999.9" Age/UL Unit 1 "1":Day "2":Month "3":Year Manual Ana. Result(R) 1 "0":Auto Ana. "1":Manual Ana. Manual Ana. Result(P) 1 "0":Auto Ana. "1":Manual Ana. Manual Ana. Result(W) 1 "0":Auto Ana. "1":Manual Ana. Manual Ana. ID 3 "000" to "127" Extend Ana. Result(W) 1 "0":Not Manual Ana. "1":Manual

Ana.

Extend Ana. Result(R) 1 "0":Not Manual Ana. "1":Manual Ana.

Manual Ana. Profile 15 Right align Extend Ana. ID 3 “000” "000" to "127" Extend Ana. Profile 15 “000000000000000” Reflagging 1 "0":Not Manual Ana. "1":Manual

Ana.

Reflagging ID 3 “000” "000" to "127" Reflagging Category 15 “000000000000000” Reserved 112 "00…00" Total(without STX,ETX) 253

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3.4.3. Analysis Data Format 3 ITEM LENGTH EXAMPLE REMARKS

Text Distinction Code1 1 "D"(fixed) Text Distinction Code2 1 "3"(fixed) Sample Distinction Code 1 "U"(fixed) Instrument's Name 16 Sequence No. 10 "0012345678" Reserved 3 "000"(fixed) Sample No. 15 "000012345678901" Right align with leading 0 padding.WBC(x10^2/uL) 6 "0": Normal

"1":Above the Limit "2":Below the Limit "3":Outside of the linearity Limit"4":unreliable

XXXXXF

RBC(x10^4/uL) 5 XXXXF HGB(g/dL) 5 XXXXF HCT(%) 5 XXXXF MCV(fL) 5 XXXXF MCH(pg) 5 XXXXF MCHC(g/dL) 5 XXXXF PLT(x10^4/uL) 5 XXXXF LYMPH%(%) 5 XXXXF MONO%(%) 5 XXXXF NEUT%(%) 5 XXXXF EO%(%) 5 XXXXF BASO%(%) 5 XXXXF LYMPH#(x10^2/uL) 6 XXXXXF MONO#(x10^2/uL) 6 XXXXXF NEUT#(x10^2/uL) 6 XXXXXF EO#(x10^2/uL) 6 XXXXXF BASO#(x10^2/uL) 6 XXXXXF RDW-CV(%) 5 XXXXF RDW-SD(fL) 5 XXXXF PDW(fL) 5 XXXXF MPV(fL) 5 XXXXF P-LCR(%) 5 XXXXF RET%(%) 5 XXXXF RET#(x10^4/uL) 6 XXXXXF IRF(%) 5 XXXXF LFR(%) 5 XXXXF MFR(%) 5 XXXXF HFR(%) 5 XXXXF PCT(%) 5 XXXXF PLT-I(only for XT-2000iV) 5 XXXX”0” PLT-O(only for XT-2000iV) 5 XXXX”0” Reserved 18 "00…00" Instrument ID(fixed) 22 “XT-2000^12345678^11001” Name of the instrument '^' PS code

'^' Serial number Total(without STX,ETX) 254

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3.4.4. Analysis Data Format 4 ITEM LENGTH EXAMPLE REMARKS

Text Distinction Code1 1 "D"(fixed) Text Distinction Code2 1 "4"(fixed) Sample Distinction Code 1 "U"(fixed) Instrument's Name 16 Sequence No. 10 "0012345678" Reserved 3 "000"(fixed) Sample No. 15 "000012345678901" Right align with leading 0 padding. DIFF-0#(x10^2/uL) 6 "0": Normal

"1":Above the Limit "2":Below the Limit

XXXXF

DIFF-1# 6 XXXXXF DIFF-2# 6 XXXXXF DIFF-3# 6 XXXXXF DIFF-4# 6 XXXXXF DIFF-5# 6 XXXXXF DIFF-6# 6 XXXXXF DIFF-7# 6 XXXXXF DIFF-8# 6 XXXXXF DIFF-1% 5 XXXXF DIFF-2% 5 XXXXF DIFF-3% 5 XXXXF DIFF-4% 5 XXXXF DIFF-5% 5 XXXXF DIFF-6% 5 XXXXF DIFF-7% 5 XXXXF DIFF-8% 5 XXXXF W/B-0#(x10^2/uL) 6 XXXXXF W/B-1# 6 XXXXXF W/B-2# 6 XXXXXF W/B-3# 6 XXXXXF W/B-4# 6 XXXXXF W/B-5# 6 XXXXXF W/B-6# 6 XXXXXF W/B-7# 6 XXXXXF W/B-8# 6 XXXXXF W/B-1% 5 XXXXF W/B-2% 5 XXXXF W/B-3% 5 XXXXF W/B-4% 5 XXXXF W/B-5% 5 XXXXF W/B-6% 5 XXXXF W/B-7% 5 XXXXF W/B-8% 5 XXXXF Reserved 18 "0000…0000"

Total(without STX,ETX) 253

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3.4.5. Analysis Data Format 5 ITEM LENGTH EXAMPLE REMARKS

Text Distinction Code1 1 "D"(fixed) Text Distinction Code2 1 "5"(fixed) Sample Distinction Code 1 "U"(fixed) Instrument's Name 16 Sequence No. 10 "0012345678" Reserved 3 "000"(fixed) Sample No. 15 "000012345678901" Right align with leading 0 padding. RET-0#(x10^4/uL) 10 "0": Normal

"1":Above the Limit "2":Below the Limit

XXXXXXXXXF

RET-1# 10 XXXXXXXXXF RET-2# 10 XXXXXXXXXF RET-3# 10 XXXXXXXXXF RET-4# 10 XXXXXXXXXF RET-5# 10 XXXXXXXXXF RET-6# 10 XXXXXXXXXF RET-7# 10 XXXXXXXXXF RET-8# 10 XXXXXXXXXF RET-1% 7 XXXXXXF RET-2% 7 XXXXXXF RET-3% 7 XXXXXXF RET-4% 7 XXXXXXF RET-5% 7 XXXXXXF RET-6% 7 XXXXXXF RET-7% 7 XXXXXXF RET-8% 7 XXXXXXF Reserved 95 00…00

Total(without STX,ETX) 288

3.4.6. Analysis Data Format 6 ITEM LENGTH EXAMPLE REMARKS

Text Distinction Code1 1 "D"(fixed) Text Distinction Code2 1 "6"(fixed) Sample Distinction Code 1 "U"(fixed) Instrument's Name 16 Sequence No. 10 "0012345678" Reserved 3 "000"(fixed) Sample No. 15 "000012345678901" Right align with leading 0 padding. DIFF-0[-] 1 "0" Not flagged "1" Flagged DIFF-0[+] 1 "0" Not flagged "1" Flagged DIFF-1[-] 1 "0" Not flagged "1" Flagged DIFF-1[+] 1 "0" Not flagged "1" Flagged DIFF-2[-] 1 "0" Not flagged "1" Flagged DIFF-2[+] 1 "0" Not flagged "1" Flagged DIFF-3[-] 1 "0" Not flagged "1" Flagged DIFF-3[+] 1 "0" Not flagged "1" Flagged

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DIFF-4[-] 1 "0" Not flagged "1" Flagged DIFF-4[+] 1 "0" Not flagged "1" Flagged DIFF-5[-] 1 "0" Not flagged "1" Flagged DIFF-5[+] 1 "0" Not flagged "1" Flagged DIFF-6[-] 1 "0" Not flagged "1" Flagged DIFF-6[+] 1 "0" Not flagged "1" Flagged DIFF-7[-] 1 "0" Not flagged "1" Flagged DIFF-7[+] 1 "0" Not flagged "1" Flagged DIFF-8[-] 1 "0" Not flagged "1" Flagged DIFF-8[+] 1 "0" Not flagged "1" Flagged W/B-0[-] 1 "0" Not flagged "1" Flagged W/B-0[+] 1 "0" Not flagged "1" Flagged W/B-1[-] 1 "0" Not flagged "1" Flagged W/B-1[+] 1 "0" Not flagged "1" Flagged W/B-2[-] 1 "0" Not flagged "1" Flagged W/B-2[+] 1 "0" Not flagged "1" Flagged W/B-3[-] 1 "0" Not flagged "1" Flagged W/B-3[+] 1 "0" Not flagged "1" Flagged W/B-4[-] 1 "0" Not flagged "1" Flagged W/B-4[+] 1 "0" Not flagged "1" Flagged W/B-5[-] 1 "0" Not flagged "1" Flagged W/B-5[+] 1 "0" Not flagged "1" Flagged W/B-6[-] 1 "0" Not flagged "1" Flagged W/B-6[+] 1 "0" Not flagged "1" Flagged W/B-7[-] 1 "0" Not flagged "1" Flagged W/B-7[+] 1 "0" Not flagged "1" Flagged W/B-8[-] 1 "0" Not flagged "1" Flagged W/B-8[+] 1 "0" Not flagged "1" Flagged RET-0[-] 1 "0" Not flagged "1" Flagged RET-0[+] 1 "0" Not flagged "1" Flagged RET-1[-] 1 "0" Not flagged "1" Flagged RET-1[+] 1 "0" Not flagged "1" Flagged RET-2[-] 1 "0" Not flagged "1" Flagged RET-2[+] 1 "0" Not flagged "1" Flagged RET-3[-] 1 "0" Not flagged "1" Flagged RET-3[+] 1 "0" Not flagged "1" Flagged RET-4[-] 1 "0" Not flagged "1" Flagged RET-4[+] 1 "0" Not flagged "1" Flagged RET-5[-] 1 "0" Not flagged "1" Flagged RET-5[+] 1 "0" Not flagged "1" Flagged RET-6[-] 1 "0" Not flagged "1" Flagged RET-6[+] 1 "0" Not flagged "1" Flagged RET-7[-] 1 "0" Not flagged "1" Flagged RET-7[+] 1 "0" Not flagged "1" Flagged RET-8[-] 1 "0" Not flagged "1" Flagged RET-8[+] 1 "0" Not flagged "1" Flagged Reserved 206 "00000…0000"

Total(without STX,ETX) 307

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3.5. QC Data Format The QC data format is used to output to XM control file, and X/L-J control files. The QC numbers indicate the following QC files. Caution: QC Data format is standardized with XT-2000i. Therefore in this format digits of RET# is 4 in spite of 5 at Analysis data format. When e-CHECK are measured, the results are 4 digits, but if another control material are used, output data are wrong.

3.5.1. QC Data Format 1 ITEM LENGTH EXAMPLE REMARKS

Text Distinction Code1 1 "D"(fixed) Text Distinction Code2 1 "1"(fixed) Sample Distinction Code 1 "C"(fixed) QC Control No. 1 See QC File Table. Year 4 "2002" YYYY Month 2 "07" MM Day 2 "04" DD Hour 2 "18" Minute 2 "09" Instrument's Name 16 RBC 4 XXXX HGB 4 XXXX HCT 4 XXXX MCV 4 XXXX MCH 4 XXXX MCHC 4 XXXX RDW-CV 4 XXXX RDW-SD 4 XXXX PLT 4 XXXX PDW 4 XXXX MPV 4 XXXX P-LCR 4 XXXX PCT 4 XXXX WBC 5 XXXXX NEUT% 4 XXXX LYMPH% 4 XXXX MONO% 4 XXXX EO% 4 XXXX BASO% 4 XXXX NEUT# 5 XXXXX LYMPH# 5 XXXXX MONO# 5 XXXXX EO# 5 XXXXX BASO# 5 XXXXX Reserved 5 "00000"(fixed) RET# 4 XXXX RET% 4 XXXX HFR 4 XXXX MFR 4 XXXX LFR 4 XXXX IRF 4 XXXX Reserved 5 "00000"(fixed) Reserved 62 "00…00"(fixed) Manual/Closed mode 1 "0":Manual "1":Closed Instrument ID(fixed) 22 “XT-2000^12345678^11001” Name of the instrument '^' PS code

'^' Serial number Total(without STX,ETX) 253

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3.5.2. QC Data Format 2 ITEM LENGTH EXAMPLE REMARKS Text Distinction Code1 1 "D"(fixed) Text Distinction Code2 1 "2"(fixed) Sample Distinction Code 1 "C"(fixed) QC Control No. 1 X See QC File Table. Year 4 "2002" YYYY Month 2 "07" MM Day 2 "04" DD Hour 2 "18" Minute 2 "09" Instrument's Name 16 WBC/BASO-X(CH) 4 XXXX WBC/BASO-Y(CH) 4 XXXX DIFF-X(CH) 4 XXXX DIFF-Y(CH) 4 XXXX Reserved 5 "00000"(fixed) Reserved 4 "0000"(fixed) Reserved 4 "0000"(fixed) Reserved 5 "00000"(fixed) Reserved 4 "0000"(fixed) Reserved 4 "0000"(fixed) RBC-O(x10^4/uL) 4 XXXX PLT-O(x10^4/uL) 4 XXXX RBC-X(CH) 4 XXXX RBC-Y(CH) 4 XXXX Reserved 4 "0000"(fixed) Reserved 4 "0000"(fixed) Reserved 4 "0000"(fixed) Reserved 4 "0000"(fixed) Reserved 125 "00…00"(fixed) Instrument ID(fixed) 22 “XT-2000^12345678^11001” Name of the instrument '^' PS code

'^' Serial number Total(without STX,ETX) 253

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3.5.3. File Corresponding to QC No. Analysis Mode Lot Control

Material Level QC File No. QC No.

Closed/ Manual

Level 1 1 1 0 Level 2 2 2 0 e-CHECK Level 3 3 3 0

Other1 7 7 0 Current

Other2 8 8 0 Level 1 11 B 0 Level 2 12 C 0 e-CHECK Level 3 13 D 0

Other1 17 b 0

Manual

New

Other2 18 c 0 Level 1 21 1 1 Level 2 22 2 1 e-CHECK Level 3 23 3 1

Other1 27 7 1 Current

Other2 28 8 1 Level 1 31 B 1 Level 2 32 C 1 e-CHECK Level 3 33 D 1

Other1 37 b 1

Closed

New

Other2 38 c 1 XbarM 0 M 0

( Note: In the Closed/Manual column, “0” indicates “Manual”, and “1” indicates “Closed”.)

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4. Communication Specifications of Analysis Information with Host Computer 4.1. Outline

XT-2000iV/XT-1800iV has a function to load analysis information (analysis order information and patient information) from the host computer, and perform the analysis automatically according to the information.

1) Analysis Information Inquiry Method

XT-2000iV/XT-1800iV has the following two inquiry methods. • Real-time inquiry is performed just before an analysis, using the sample ID number as a

key word, read from the bar code label attached to a sample tube. • Batch inquiry is performed before an analysis by specifying a rack number. The key

words in this case are the rack number and the tube position.

2) Sample Information Inquiry Setting Key: Sample ID Rack No./Tube Pos. Real-time Inquiry (Manual Mode) : [Sample ID] Real-time Inquiry (Sampler Mode) : [Key]

4.2. Serial Communication The bit serial voltage type, which conforms to the RS-232C interface, is used for host computer output of XT-2000iV/XT-1800iV. The serial interface port for the connection with the host computer is on the rear panel of the IPU.

1) Connector - Connect to the host computer output connector on the rear panel of the IPU. - Use a 9-pin, D-SUB female connector. - Pitch of connector fixing screws adopts the size in inch.

2) Connector Signal

Pin No. Signal Name Signal Direction

1 2 Receive Data (RxD) To XT-2000iV/XT-1800iVfrom HOST 3 Transmit Data (TxD) From XT-2000iV/XT-1800iVto HOST 4 Data Terminal

Ready (DTR) From XT-2000iV/XT-1800iVto HOST

5 Signal Ground (SG) 6 Data Set Ready (DSR) To XT-2000iV/XT-1800iVfrom HOST 7 Request to Send (RTS) From XT-2000iV/XT-1800iVto HOST 8 Clear to Send (CTS) To XT-2000iV/XT-1800iVfrom HOST 9

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3) Communication Format The format is start-stop asynchronous half duplex (asterisk indicates setting at shipment from factory), and adjustment can be made as follows.

Baud Rate 600, 1200, 2400, 4800, *9600, 14400, 19200, 38400 (BPS) Code 7-bit, *8-bit Stop Bit *1-bit, 2-bit Parity Bit *None, Even, Odd Class Class A, *Class B Interval 0, 1, *2, 3, 5, 7, 10, 15 (seconds)

4) Signal Level

Level Data Signal Control Signal

+3V or Higher Logic "0," Start Bit ON -3V or Lower Logic "1," Stop Bit OFF

5) Communication Format

(1) Code ASCII codes are used for all the output to the computer.

(2) Text Format "STX" is sent at the beginning, and "ETX" is sent at the end of data. STX: 02H, ETX: 03H

(3) Transmission Procedure The transmission method is class B (fixed). Make sure to set it class B. If the class A is set, correct communication is not possible. The procedure to make inquiry on analysis information from XT-2000iV/XT-1800iV to the host computer is as follows: • Analysis information inquiry text is sent from XT-2000iV/XT-1800iV. • The host computer returns NAK if an error occurs with the data reception, and returns ACK when there is no error, and then send the analysis information corresponding to the inquiry. • In the case of NAK, XT-2000iV/XT-1800iV resends the analysis information inquiry text. In the case of ACK, after receiving the analysis information text, XT-2000iV/XT-1800iV sends ACK when there is no error with the received data, or sends NAK if there is an error. • The host computer closes the communication for one set of analysis information when the returned response is ACK, or resends the analysis parameter text if the response is NAK.

[XT-2000iV/XT-1800iV] [Computer] Sends sample information inquiry text. (Resends if NAK.) (Resends up to 3 times.) Sends NAK if there is an error. or ACK when there is no error.

Sends NAK if there is an error. or ACK when there is no error. Sends sample information text for the inquired samples. (Resends if NAK.) (Resends up to 3 times.)

ACK: 06H, NAK: 15H

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(4) Transmission Error

If a transmission error is detected, the transmission is interrupted and the error message is displayed on the IPU. The operator must recover the error. Cases of errors are as follows.

Error name Descriptions IPU Action

to be taken Transmission Error Detects Frame Error, Parity Error or Over-run Error, when

receiving. Interrupt

Off-line HC’s control signal “DSR” is OFF. Interrupt ACK Time-out After data transmission, no response was received from HC in

15 seconds. (Class B only) Interrupt

(No error) After data transmission, NAK was received from HC. (Class B only)

Re-send

(No error) After data transmission, a response other than ACK and NAK was received from HC. (Class B only)

Re-send

No. of re-send over After data transmission, the 4th response other than ACK was received from HC. (Class B only)

Interrupt

Transmission Time-out

When sending, control signal CTS does not become active in 5 seconds. (only when RTS/CTS control is activated)

Interrupt

STX Time-out (1) After HC’s requested to send, STX was not received from HC in 15 seconds. (Class A only) (2) After HC’s requested to send, STX was not received from HC 15 seconds after ACK was received. (Class B only) (3) Within 15 seconds after receiving the sub-text in the previous text, STX of the following sub-text was not received. (Class A only) (4) Within 15 seconds upon sending ACK after receiving the sub-text in the previous text, STX of the following sub-text was not received. (Class B only)

Interrupt

ETX Time-out ETX was not received within 15 seconds after receiving STX. Interrupt

(5) Inquiry Timing •Real-time inquiry by sample ID number At the time when a sample ID number is read from the bar code attached to the tube, the inquiry is performed. If XT-2000iV/XT-1800iV already has the analysis information, the inquiry is not performed. After sending out the analysis information inquiry text, wait while receiving ACK and receiving the analysis information text, then blood is absorbed after that. •Batch inquiry by rack number and tube position At the time when a rack number is specified by the IPU program of XT-2000iV/XT-1800iV, the inquiry is made for the tube positions from 1 through 10 for the specified rack. There is not time limit to receive the analysis information text after sending the analysis information inquiry text.

(6) Transmission Interval

The transmission interval from receiving analysis information text from the host computer and sending out ACK until the next sending of analysis information inquiry text, can be set by the IPU program of XT-2000iV/XT-1800iV. This transmission interval is common with that of analysis data.

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(7) Note on Analysis Data Automatic Output When the analysis data is set to be output automatically by XT-2000iV/XT-1800iV, the communication of analysis information and the output of analysis data may be performed alternately. In this case when two different types of information are handled simultaneously, the response of the host computer may become slower. Therefore, set the instrument to make the batch output, not the automatic output to avoid slow response of the host computer.

4.3. TCP/IP Communication The network interface conforms to IEEE802.3, is used for host computer output of XT-2000iV/XT-1800iV. The network port for the connection with the host computer is on the rear panel of the IPU.

1) Network interface layer

The network interface conforms to IEEE802.3. Communication is performed by 10Base-T. The RJ45 socket is used as a hub for XT-2000iV/XT-1800iV connection The cable of UTP category 5 is used for communication.

2) TCP/IP

• Before communication, the text format including STX and ETX is set to the TCP's data unit. • The IP address of XT-2000iV/XT-1800iV's Main unit is set (The default value is 192.168.28.142 and this value may be changed on Windows networking setting and Main unit by service engineer.) • The IP address for XT-2000iV/XT-1800iV's host communication is set. ( This value may be changed on IPU setting screen and we have to use other IP address without 192.168.28.* ) • The TCP port number for XT-2000iV/XT-1800iV's host communication is set. (The default value is 5000 value may be changed on IPU setting screen.)

3) TCP Connection

The host computer is defined as server, and the XT-2000iV/XT-1800iV as client for connection. The XT-2000iV/XT-1800iV checks the connection when the system starts. When the connection fails, the XT-2000iV/XT-1800iV repeats the connection at regular intervals. If the server shuts down after the connection, the XT-2000iV/XT-1800iV does not perform the connection again.

4) Application Layer

Communication is performed according to the bit serial interface class A.

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5) Text and Communication Protocol (1) Communication Control Character

ASCII codes are used for all the output to the computer. (2) Text Format

“STX” is sent at the beginning, and “ETX” is sent at the end of data.

6) Communication Error Processing When a communication error occurs, the communication is stopped and the error is displayed on the IPU. The restoration of communication can be made by the operator. A communication error occurs in the case mentioned below. • No response is made from the computer within 30 seconds after the data transmission.

7) Communication Timing

Depending on the settings of the IPU, it is possible to select between communication per analysis cycle and communication of the stored data in a batch.

4.4. Text Format There are text formats for "Analysis information inquiry" and "Analysis information" regarding the communication of analysis information between XT-2000iV/XT-1800iV and the host computer. And these are identified by Text Distinction Code I. • The Text Distinction Code I of the "Analysis information inquiry" sent from XT-2000iV/XT-1800iV to the host computer is always "R." • The Text Distinction Code I of the "Analysis information" sent from the host computer to XT-2000iV/XT-1800iV is always "S."

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4.5. Analysis Information Inquiry Format

ITEM LENGTH EXAMPLE REMARKS Text Distinction Code1 1 "R"(fixed) Inquiry Mode 1 "1": Real-time inquiry by Sample ID

number as the key "2":Batch inquiry by rack No. and tube position as the key.

Reserved 3 "000"(fixed) All letters are "0" Inquiry Sample ID No. 15 "____12345678901" Right align.. Reserved 2 "00"(fixed) Rack Number 6 "001234" 0 padding Position of Tube 2 "08" 0 padding Reserved 31 "00…00"(fixed) All letters are "0" Total(without STX,ETX) 61

(1) Output Order

The upper items are sent first, and upper digits of data are sent first. Zero-suppression is not carried out.

(2) Inquiry Mode The mode of inquiry is indicated. "1": Real-time inquiry by sample ID number as the key word. "2": Batch inquiry by rack No. and tube position as the key words.

(3) Inquiry Sample ID Number This parameter becomes effective with the real-time inquiry by sample ID number as the key word. It consists of 15-digit number, but may include hyphen "-" (2DH) between numerals depending on the usage. The hyphen "-" is included in 15 digits.

(4) Rack No. This parameter becomes effective with the batch inquiry by rack No. and tube position as the key words. This is the number assigned to a sample rack. It contains of 6-digit number.

(5) Tube Position This parameter becomes effective with the batch inquiry by rack No. and tube position as the key words. It consists of number from 1 to 10 for an analysis position on a sample rack.

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4.6. Analysis Information Format 4.6.1. Analysis Information Format 1

ITEM LENGTH EXAMPLE REMARKS Text Distinction Code1 1 "S"(fixed) Text Distinction Code2 1 "1"(fixed) Information Status 1 "0": Not registered

"1":Registered "2":Quality Control

Date Ordered 8 "20020704" YYYYMMDD Reserved 3 "000"(fixed) Sample ID No. 15 "____12345678901" Right align. Reserved 2 "00"(fixed) Rack Number 6 "001234" Tube Position 2 "08" Inquiry Mode 1 "1": Real-time inquiry by Sample ID

number as the key "2": Batch inquiry by rack No. and tube position as the key.

Animal Species 2 “00” “01”:Mouse “02”:Rat “03”:Rabbit “04”:Dog “05”:Monkey “07”:Other“08”Other1 “09”:Other2 “10”:Other3 “11”:Other4 “12”:Other5 “13“:Horse “14“:Cat “15“:Guinea pig

Category 3 “001”:Rat std “002”:Mouse std “003”:Monkey std “004”:Dog std “005”:Rabbit std “006”:Other1 std “007”:Other2 std “008”:Other3 std “009”:Other4 std “010”:Other5 std “129”:Other std “130“:Horse std “131“:Cat std “132“:Guinea pig std

Reserved 52 "000000000000000000"(fixed) Reserved 8 "000000000000000000"(fixed) Reserved 20 "000000000000000000"(fixed) Reserved 20 "000000000000000000"(fixed) Sample Comments 40 Reserved 18 "000000000000000000"(fixed) WBC 1 "0":Not analyze "1":Analyze RBC 1 "0":Not analyze "1":Analyze HGB 1 "0":Not analyze "1":Analyze HCT 1 "0":Not analyze "1":Analyze MCV 1 "0":Not analyze "1":Analyze MCH 1 "0":Not analyze "1":Analyze MCHC 1 "0":Not analyze "1":Analyze PLT 1 "0":Not analyze "1":Analyze LYMPH% 1 "0":Not analyze "1":Analyze MONO% 1 "0":Not analyze "1":Analyze NEUT% 1 "0":Not analyze "1":Analyze EO% 1 "0":Not analyze "1":Analyze BASO% 1 "0":Not analyze "1":Analyze LYMPH# 1 "0":Not analyze "1":Analyze MONO# 1 "0":Not analyze "1":Analyze NEUT# 1 "0":Not analyze "1":Analyze EO# 1 "0":Not analyze "1":Analyze BASO# 1 "0":Not analyze "1":Analyze

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RDW-CV 1 "0":Not analyze "1":Analyze RDW-SD 1 "0":Not analyze "1":Analyze PDW 1 "0":Not analyze "1":Analyze MPV 1 "0":Not analyze "1":Analyze P-LCR 1 "0":Not analyze "1":Analyze Reserved 2 "00"(fixed) RET% 1 "0":Not analyze "1":Analyze RET# 1 "0":Not analyze "1":Analyze IRF 1 "0":Not analyze "1":Analyze LFR 1 "0":Not analyze "1":Analyze MFR 1 "0":Not analyze "1":Analyze HFR 1 "0":Not analyze "1":Analyze Reserved 1 "0"(fixed) PCT 1 "0":Not analyze "1":Analyze Reserved 1 "0"(fixed) Reserved 1 "0"(fixed) PLT-O 1 "0":Not analyze "1":Analyze Reserved 14 "00000000000000"(fixed)

Total(without STX,ETX) 253

4.6.2. Analysis Information Format 2 ITEM LENGTH EXAMPLE REMARKS

Text Distinction Code1 1 "S"(fixed) Text Distinction Code2 1 "2"(fixed) Information Status 1 "0": Not registered

"1":Registered "2":Quality Control

Date Ordered 8 "20020704" YYYYMMDD Reserved 3 "000"(fixed) Sample ID No. 15 "____12345678901" Right align. Reserved 2 "00"(fixed) Rack Number 6 "001234" Tube Position 2 "08" Inquiry Mode 1 "1": Real-time inquiry by Sample ID

number as the key "2": Batch inquiry by rack No. and tube position as the key.

Reserved (Patient ID No.) 16 "0000000000000000" Reserved(Patient Comment) 100 "00…00” Reserved 97

Total(without STX,ETX) 253

1) Output Order The upper items are sent first, and upper digits of data are sent first. Zero-suppression is not carried out.

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2) Information Status This parameter indicates if the inquired analysis information is registered. If the required sample is not registered, make sure to return "0" (No order registered) in the analysis information text. "0": No order registered "1": Order registered "2": Quality control

3) Date Ordered This parameter indicates the requested date of analysis of the inquired sample. "YYYYMMDD" YYYY: Year, MM: Month, DD: Day

4) Sample ID Number In the case of real-time inquiry by sample ID number as the key word, this number becomes the same with that in the inquiry text. In the case of batch inquiry by rack No. and tube position as the key words, the sample ID number corresponding to the specified rack No. and tube position will be assigned. It consists of 15-digit number or alphabet, but may include hyphen "-" (2DH) between numerals depending on the usage. The hyphen "-" is included in 15 digits.

5) Rack No. In the case of batch inquiry by rack No. and tube position as the key words, this number becomes the same with that in the inquiry text. In the case of real-time inquiry by sample ID number as the key word, no particular setting is used. This number is assigned to a sample rack, and consists of 6 digit number.

6) Tube Position

In the case of batch inquiry by rack No. and tube position as the key words, this number becomes the same with that in the inquiry text. In the case of real-time inquiry by sample ID number as the key word, no particular setting is used. This is the analysis position of the inquired sample in the sample rack, and consists of number from 1 to 10.

7) Inquiry Mode

The mode of inquiry is indicated. "1": Real-time inquiry by sample ID number as the key word. "2": Batch inquiry by rack No. and tube position as the key words.

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8) Animal Species The species of inquiry is indicated. ”01”: Mouse ”02”: Rat ”03”: Rabbit ”04”: Dog ”05”: Monkey ”07”: Other ”08”: Other1 ”09”: Other2 ”10”: Other3 ”11”: Other4 ”12”: Other5 “13”: Horse “14”: Cat “15”: Guinea pig

9) Category The category of inquiry is indicated. ”001”: Rat std ”002”: Mouse std ”003”: Monkey std ”004”: Dog std ”005”: Rabbit std ”006”: Other1 std ”007”: Other2 std ”008”: Other3 std ”009”: Other4 std ”010”: Other5 std ”129”: Other std “130”: Horse std “131”: Cat std “132”: Guinea pig std

10) Sample Comments

This is the comments for the inquired sample, and consists of up to 40 alphabets. When no sample comment is available, enter a space (20H).

Note: Sample comments cannot be used in the real-time order inquiry.

11) Order Information

This indicates the analysis order information for each analysis parameter. "0": Not analyze "1": Analyze

(End of Documents)