quick start guide - st. michael's...
TRANSCRIPT
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Page 1 of 12
to briefly deand acquire settings isition setting
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7. To load
8.
9.
10.
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11.
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12. Click
main toolbarsition Setup
d a previous
Click If the settin
click
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atively, you c
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to beg
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s saved proto
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open the do
close the do
Main Taskb
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Page 2 of 12
or in the m
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dows for theatabase, hig
oor and plac
oor
bar to open a
plate name,
2
main menu s
in Plate
e appropriatehlight the pr
ce the plate i
and close th
and descrip
select Scree
Acquisition
e .hts file. rotocol and
in the in the
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n Setup
system
ired
ate
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II. Test A
1. Open Pl
2.
In the plmove th
3.
Test the
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4.
Adjust th
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*NOTE*options
5. When yo
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Acquis
late Acquisate and sitee plate to th
e acquisition
to pe
to pewill be acqui
to pebeen activate
he acquisitio
Adjust the fo
Adjust the ex
* Click on the
ou have opti
Molecular De
Click
to begin
sition S
ition Setup section of P
hat position (
settings by
erform a larg
erform a focured)
erform an aued, all plane
on settings, i
ocus offset b
xposure time
e wavelength
imized settin
evices recom
to sea
n acquiring t
P
ettings
Plate Acquis(indicated by
clicking
ge range aut
us and snap
utofocus andes will be acq
f necessary
y clicking
e by clicking
h name to o
ngs, click
mmends ena
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the plate
Page 3 of 12
s
sition Setupy a dark gree
tofocus and
p image rout
d snap imagequired)
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or a
pen the corr
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p, right-clicken color)
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adjusting the
or chang
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for all wave
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ing the num
wavelength
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red well and
n activated, a
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anually
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all planes
Z series has
ly
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I
12
3
4
5
6
7
III. Def
1. Open P2. Select
3.
Select i. ii. iii.
4.
Adjust
bottomproces
5. Select plate ty
6.
Select
7. Select
fine Ne
Plate Acquithe Configuthe ObjectivSelect the aSet binningIf necessar
the objective
m thickness). ss.
the Plate taype from thethe Sites toSingle SiteFixed numspacing of ssite to moveAdaptive aindicated bychoose wavMulti-well: automaticaCustom fieuseful when
the Acquisi
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sition Setupure tab ve and Camappropriate
g (2 for cell cry set gain (g
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On the Run
ab and selecte drop-down o Visit tab ane: image one
mber of sitessites. Left-cle the plate t
acquisition: y the user. Tvelength, sizcollect multlly
eld of view (n the field of
ition tab to s
P
uisition
p
mera tab magnificatio
counting andgain of 2 for
collar if nec
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t the approplist
nd select thee site per wes: image the lick on sites o that site pocollect the m
The Adaptiveze and threstiple wells wi
(%): reduce f view covers
select Autofo
Page 4 of 12
n Settin
on from the dd cell scoringbinning of 1
cessary (sett
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priate
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number of sto select (grosition (darkminimum nue Acquisitionhold settingsithin one ima
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ocus and Ac
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e number ofter selected sitereen) and dek green) umber of siten section wils, and desireage which is
each image the site/wel
cquisition op
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match phys
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sical plate
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number andck on any
cell count ser to ells single wells
red. This is
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s
8
9
1
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8.
Autofoc
9.
Acquis
10.
Other o
11.
Select
i.
ii.
iii.
iv.
v.
vi.
cus options:Always selefocusing Enable imasamples orplanes from
sition optionsEnable Acqtimelapse eEnable Acqacquisition
options: If running aenable this tab If an analysenable AnaAcquisitio*NOTE* thito be in AutPowerCoreTo correct fPerform shthe approprcorrection ithe Autofoc
Set Well tohowever wh
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age-based fr those with dm site-to-sites: quire Time sexperiments quire Z serie
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e software for uneven bhading corrriate directormages are scus tab:
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ocus to g thin-bottom
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Page 5 of 12
d
r thick al
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to correspon
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12. Select waveleto acqu
13.
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iv.
v. vi.
15.
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the Waveleengths (coloruire plate and sit well) and/o
the W1 (wavSelect the d
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iring a Time
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nd select the transmitted
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est the new brightness
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ct how often
Page 6 of 12
e number of light that yo
uisition Setuto that positi
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erform an au
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with ecrease expto acquire th
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selected weore continuin
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wavelength
elect the Timme points de between etal time of thes for: the next: enell before ac
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Page 7 of 12
ate setting fo
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melapse tabdesired each time pohe experimen
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21.
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22.
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the Journale journals wsary to enab
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Page 8 of 12
s tab
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Page 9 of 12
cquisition Analysis menus
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name, PI, etar experimenate server fo
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tc. All your pnt or image sto
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1
2
3
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6
7
IV. Rev
1. In the Acqui
2. On the
3.
4.
In the acrosswavele
5.
If there
approThe im
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7.
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main toolbaisition Setu
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Page 10 of 12
n an An
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Page 11 of 12
appropriate b
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Page 12 of 12
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