quantitativeanalysis of potassimsorbate …saitenhyogo.kir.jp/chemgroup/scienceconference.pdf ·...
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![Page 1: QUANTITATIVEANALYSIS OF POTASSIMSORBATE …saitenhyogo.kir.jp/chemgroup/ScienceConference.pdf · Bibliography • 増尾清著,消費者にできる食品簡易テスト,誠文堂新光社(1980)(P.30,31)](https://reader033.vdocuments.us/reader033/viewer/2022053022/604e31c325392f28cb6de068/html5/thumbnails/1.jpg)
QUANTITATIVE ANALYSISOF
POTASSIM SORBATEIN PROCESSED FOODSIN PROCESSED FOODS
NOBUMOTO Miyu
FURO Mizuki
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Processed meats
– such as bacon, sausages and ham– do cause cancer , according to the World Health according to the World Health Organization(WHO).
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What is Potassium Sorbate(C6H7KO2)?
• It is a preservative.
…preserve food from mold and bacteria.
• It is in processed foods.
<example>ham, cheese, sausage, boiled fish <example>ham, cheese, sausage, boiled fish paste
• Nitrite + Potassium Sorbate
→ a Carcinogen
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Back Ground
Is it safe for humans?
Goal To do a quantitative analysis of potassium sorbate in processed foods
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Method
• Cutting food
• Making a test solutionMaking a test solution
• Colorimetric analysis
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boiled fish paste
0.78 mg 7~14 mg
Experimental Value Actual Value
This is our result of the amountof potassium sorbate in 10 g food
0.78 mg 7~14 mg
wiener
0.74 mg 12 mg
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Why???
• Cutting food
• Making a test solutionMaking a test solution
• Colorimetric analysis
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We tried 3 method for extracting potassium sorbate(C6H7KO2).
The methods wereThe methods were1. Evaporation2. The cut size of boiled fish paste3. pH
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Evaporation 1
Does potassium sorbate(C6H7KO2)evaporate by heating on extraction ?
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How to make the test solution
1. Dice 10g of food.
2. Put it into 25 mL of water.
3. Warm the beaker in hot water at 60 ℃.4. Scoop up the clear layer at the top.4. Scoop up the clear layer at the top.
This is the test solution.
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Method 1
pure water+
pure water
+
potassium sorbateheating
quantitative analysis
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MethodMeasure the peak wavelength
of the spectrum.(→252 nm)
And measure absorbance of 252 nm.
spectrometer
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Result 1
ABS
pure water 0.00
Absorbance at 252 nm
pure water 0.02
potassium sorbate 1.72
potassium sorbate 1.74
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Consideration 1• We think the concentration of
potassium sorbate rises becausewater evaporates from heating.
• Potassium sorbate doesn’t evaporate.• Potassium sorbate doesn’t evaporate.
Before After
g/㎥
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the cut size of boiled fish paste 2
Does the cut size of boiled fish paste affect the extraction ofpotassium sorbate?
mash 5 mm3 10 mm3
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Method 2
pure water 25 mL
boiled fish paste(10 g)
• 10 mm3
• 5 mm3
mash
colorimetric analysis
• mash
extract heating 60℃ 20 minutes
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Colorimetric Analysis:Reagent A(an oxidizer)
a water solution of K2Cr2O7(0.0017 mol/L)
H2SO4(0.15 mol/L)
Mixture by volume for 1:1
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10 mL of NaOH(1.0 mol/L)
Colorimetric Analysis:Reagent B(Color fixative)
11 mL of HCl(1.0 mol/L)
At last, add pure water to 100 ml
0.50 g of C4H4N2O2S+20 mL of pure water
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Colorimetric Analysis:Final step
①2 mL of a test solution + Reagent A(a oxidizer) → boiled for 5 minutes at 60 ℃
②The solution + Reagent B(Color fixative)
→ boiled for 10 minutes at 60 ℃
③After that, measure absorbance of 532 nm.
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Result 2
Absorbance at 532 nm ABS
Pure water 0.00
5 mm3 2.39
10 mm3 2.35
mash 2.01
Abs of Mash is lower than the 5 mm3 cubes .
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Discussion 2•Mash can’t extract potassium sorbate as well as 5 mm3.
•We think there is a best cut sizeof boiled fish paste for extracting potassium sorbate.
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Discussion: 5mm3 and 10mm3
potassium sorbate extraction
>>
5mm3
10mm3
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Discussion: mash and 5mm3
potassium sorbate extraction
<<
mash5mm3
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pH 3
Does pH affect extraction of potassium sorbate?
pH
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pH 3
low pH Decomposed molecules decrease.Absorbance is lower.
high pH Decomposed molecules increase. Absorbance is higher.
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Method 3
pure water 22.5 mL+
buffer solution 2.5 mL++
boiled fish paste(5mm3) 10 g
extract heating 60℃ 20 minutes
colorimetric analysis
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Result 3Absorbance at 532 nm
1st time 2nd time
1/10
pH 4.1 0.30 0.45pH 4.1 0.30 0.45
pH 6.82 0.26 0.36
pH 9.18 0.27 0.38
Steamed fish paste and Pure water(pH 6.5)
0.34 0.49
Pure water 0.00 0.00
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Consideration 3
There is little difference because of pH.
→pH doesn't affect the extraction.→pH doesn't affect the extraction.
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Conclusion
• Heating
• SizeDo not affect extraction of potassium sorbate very much.• pH very much.
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Future work
•Reconsider extraction methodof Potassium Sorbate .
•Do ultraviolet quantitative analysis.
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