quantitative study of the phytoplankton of lake michigan at … · 2017-05-02 · recorded in june....

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Butler University Botanical Studies Volume 5 Butler University Botanical Studies Article 3 Quantitative study of the phytoplankton of Lake Michigan at Evanston, Illinois Kenneth E. Damann Butler University Follow this and additional works at: hp://digitalcommons.butler.edu/botanical e Butler University Botanical Studies journal was published by the Botany Department of Butler University, Indianapolis, Indiana, from 1929 to 1964. e scientific journal featured original papers primarily on plant ecology, taxonomy, and microbiology. is Article is brought to you for free and open access by Digital Commons @ Butler University. It has been accepted for inclusion in Butler University Botanical Studies by an authorized administrator of Digital Commons @ Butler University. For more information, please contact [email protected]. Recommended Citation Damann, Kenneth E. (1941) "Quantitative study of the phytoplankton of Lake Michigan at Evanston, Illinois," Butler University Botanical Studies: Vol. 5, Article 3. Available at: hp://digitalcommons.butler.edu/botanical/vol5/iss1/3

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Page 1: Quantitative study of the phytoplankton of Lake Michigan at … · 2017-05-02 · recorded in June. The g-reatest weekly change in yield occurred be tween the last two weeks of July

Butler University Botanical Studies

Volume 5 Butler University Botanical Studies Article 3

Quantitative study of the phytoplankton of LakeMichigan at Evanston, IllinoisKenneth E. DamannButler University

Follow this and additional works at: http://digitalcommons.butler.edu/botanicalThe Butler University Botanical Studies journal was published by the Botany Department of ButlerUniversity, Indianapolis, Indiana, from 1929 to 1964. The scientific journal featured original papersprimarily on plant ecology, taxonomy, and microbiology.

This Article is brought to you for free and open access by Digital Commons @ Butler University. It has been accepted for inclusion in Butler UniversityBotanical Studies by an authorized administrator of Digital Commons @ Butler University. For more information, please contact [email protected].

Recommended CitationDamann, Kenneth E. (1941) "Quantitative study of the phytoplankton of Lake Michigan at Evanston, Illinois," Butler UniversityBotanical Studies: Vol. 5, Article 3.Available at: http://digitalcommons.butler.edu/botanical/vol5/iss1/3

Page 2: Quantitative study of the phytoplankton of Lake Michigan at … · 2017-05-02 · recorded in June. The g-reatest weekly change in yield occurred be tween the last two weeks of July

T he Bu t le r Un iv e r s i ty Bo ta n ic a lS tu d ie s jo u r n a l w a s p u b li s he d by t he Bo t-a n y De p a r tm e n t o f B u t le r U niv e r si ty, I n di a n a po li s, I n di a n a, f ro m 1 9 2 9 to1 9 6 4. T h e s ci e n ti fi c jo u r n a l fe a t u re d o rig i n a l p a pe r s p ri m a ri l y o n p l a n te co lo g y, t a xo no m y, a n d m i c ro bi o lo g y . T he p a pe r s co n t ai nv a l u a b lehi s to ri c a l s t u di e s, e s pe ci a l l y f l o ri s ti c s u rv e y s t h a t do c u m e n t I n di a n a ’ sv e g e t a ti o n i n p a s t de c a de s . A u t ho r s we re B u t le r f a c u l ty, c u r re n t a n dfo rm e r m a s te r ’ s de g re e s t u de n t s a n d u n de rg r a d u a te s, a n d o t he r I n di a n abo t a ni s t s . T he jo u r n a l w a s s t a r te d by S t a n le y C ai n, no te d co n se rv a ti o nbi o lo g i s t, a n d e di te d t h ro ug h m o s t o f i t s y e a r s o f p ro d u c ti o n by R a y C.F ri e s ne r, B u t le r ’ s fi r s t bo t a ni s t a n d fo u n de r o f t he de p a r tm e n t i n 1 9 1 9 .T he jo u r n a l w a s di s t ri b u te d to le a r ne d so ci e ti e s a n d li b r a ri e s t h ro ug he x c h a ng e .D u ri ng t he y e a r s o f t he jo u r n a l ’ s p u b li c a ti o n, t he B u t le r U niv e r si ty Bo t-a n y De p a r tm e n t h a d a n a c tiv e p ro g r a m o f re se a r c h a n d s t u de n t t r ai ni ng .2 0 1 b a c he lo r ’ s de g re e s a n d7 5 m a s te r ’ s de g re e s i n Bo t a n y we re co n -fe r re d d u ri ng t hi s pe ri o d . T hi r ty - fiv e o f t he se g r a d u a te s we n t o n to e a r ndo c to r a te s a t o t he r i n s ti t u ti o n s .T he Bo t a n y De p a r tm e n t a t t r a c te d m a n y no t a b le f a c u l ty m e m be r s a n ds t u de n t s . Di s ti ng ui s he d f a c u l ty, i n a d di ti o n to C ai n a n d F ri e s ne r, i n -c l u de dJ o h n E . Po tz g e r, a fo re s t e co lo g i s t a n d p a l y no lo g i s t, Wi l l a r dN e l -so n C l u te , co - fo u n de r o f t he Am e ri c a n Fe r n So ci e ty, M a ri o n T. H a l l, fo r-m e r di re c to r o f t he M o r to n A r bo re t u m , C. M e rv i n P a l m e r, Re x We b s te r,a n dJ o h n Pe l to n . So m e o f t he fo rm e r u n de rg r a d u a te a n d m a s te r ’ s s t u -de n t s w ho m a de a c tiv e co n t ri b u ti o n s to t he fi e l d s o f bo t a n y a n d e co lo g yi n c l u de D wig h t . W . Bi l li ng s, F a y Ke no y e r D ai l y, Wi l li a m A. D a i l y, Re x fo r dD a u de n m i re , F r a n ci s H ue be r, F r a n k M c Co rm i c k, S co t t M c Co y, Ro be r tPe t ty, Po tz g e r, H e le ne S t a r c s, a n d T he o do re S pe r ry . C ai n, D a u be n m i re ,Po tz g e r, a n d Bi l li ng s se rv e d a s P re si de n t s o f t he E co lo g i c a l So ci e ty o fAm e ri c a .Re q ue s t s fo r u se o f m a te ri a l s, e s pe ci a l l y fig u re s a n d t a b le s fo r u se i ne co lo g y te x t bo o k s, f ro m t he Bu t le r Un iv e r s i ty Bo ta n ic a lS tu d ie s co n -ti n ue to be g r a n te d . Fo r m o re i n fo rm a ti o n, v i si t w w w. b u t le r. e d u /

Page 3: Quantitative study of the phytoplankton of Lake Michigan at … · 2017-05-02 · recorded in June. The g-reatest weekly change in yield occurred be tween the last two weeks of July

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QUANTITATIVE STUDY OFTHE PHYTOPLANK­TON OF LAKE MICHIGAN AT EVANSTON, ILLINOISt

By KENNETH E. DAi\Il\NN

Beginning May 9, 1937, and continuing to May 3, 1938, Daily made regular weekly quantitative eollections of the phytoplankton of Lake Michigan at Evanston, Illinois. Several ecological factors were studied in relation to their effect on the periodicity of the phytoplank­ton. However, due to the various fluctuations of physical and chem­ical factors, the data for anyone year scarcely represent, in them­selves, a complete picture of either the phytoplankton crop or its periodicity. It was therefore deemed advisable to continue the in­itiated quantitative survey over a longer period. At this point, De­cember 1939, two years 0 f collecting have been completed and an attempt has been made to summarize and compare the data for that period.

Although several papers have been published on the plankton of Lake Michigan, none has dealt with quantitative collections over any extended period of time. Briggs (1872) listed 45 species of diatoms found in Lake Michigan. Thomas amI Chase (1887) brought to­gether a long list of 215 species of diatoms found over a period of 16 years in the water supply of the .city of Chicago. Ward (1896) worked in the Traverse Bay Region on the relation of plankton and bottom organisms to the whitefish. Jennings, Thompson and Kofoid later added appendices, on rotifers, phytoplankton, and protozoa re­spectively to Ward's publication.

Eddy (1927) published quantitative data obtained from two series of collections which were taken from November 1887 throug'h October 1888 amI in October 1926 and May 1927 at various points along Lake Michigan, mostly in the immediate vicinity of Chicago. A total of 119 species were found, sixty of which were phytoplankters and fifty-nine were zooplankters. A comparison of the more recent collections with those made forty years previous, showed that very

'A portion of the work done in partial fulfillment of the requirements for the Master of Science Degree in Northwestern University which is a continua­tion of the study initiated by Daily: Butler University Botanical Studies 4 (6) 1938.

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little change had occurred in the general composition of the plankton. Diatoms were found to predominate at all times and constituted the majority of the organisms of the plankton.

Bayliss and Gerstein (1929) in a two year study of the phyto­plankton and zookplankton in the lake water of the Chicago water supply found that peaks of plankton abundance were reached in May and Octoher of 1927 bnt only in September of 1928. The writer is at present working on continuous monthly plankton records begin­ning in 1926 which were made available by A. E. Gorman and H. H. Gerstein of the Water Puri fication Department of Chicago, Illinois. It is hoped that such records will g-ive us a more complete picture of phytoplankton periodicity and possibly suggest clues as to its explanation.

Both Eddy (1930) and Roach (1932) report a direct correlation of plankton abundance with temperature. However, Pear~all (1923) fails to stress temperature as a leading role in diatom periodicity, but is inclined to believe that the deficiencies of oxygen, nitrates, silica and calcium are usually limiting factors. In 1932, Pearsall takes a more defini tc stand when he writes that diatoms occur in winter and spring when nitrates. phosphates, and silica are in abund­ance, and that green algae occur in the summer when nitrates and phosphates are low.

Ahlstrom (1936) published a very complete account of the deep water and inshore plankton of Lake Michigan as disclosed in 115 samples taken during 1930 and 1931 by the United States Bureau of Fisheries from stations scattered well over the entire area of the lake. The inshore collections were made at Evanston, Illinois, be­tween October 3, 1931 and June 20, 1932. He recognized the need for quantitative studies to establish the existence of seasonal periodi­city which he detected in the qualitative collections.

Daily (1937) initiated this quantitative study of the phytoplank~

ton of Lake Michigan at Evanston, Illinois. He considered tempera­ture as being important in optimum growth but not significant enongh to he the controlling factor of periodicity. Further correlations were made with hours of sunlig-ht. turbidity, hydrogen ion concentration, and bacteria.

28

During tl pleted, regula! breakwater a: Sedgwick-Ra sists 0 f f ilte mesh bolting isms from t~

fOltnd in the. represented'

No. of fields a 1 ml countll cell 1 mm d

No. of fields counted

The Whipple ocular any oM to one square to 10 cc and ten fields we~

Thus:

Quantitati­from N'Ovem senting 5 c1as tion, averagin ccae, and ChI only 3%, 2% a small fracti complete data ton are repre and water· ten1

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METHODS AND 1'1ATERIALS

During the two years of this phytoplankton study thus far com­pleted, regular weekly quantitative collections were made from a breakwater adjoining the Northwestern University campus. The Sedgwick-Rafter method was used exclusively. This method con­sists of filtering water samples through sand supported upon 200 mesh bolting cloth disks and then (aleulating the number of organ­isms from the concentrate. The formula for such calculation is fonnd in the Standard Method of 'Vater Analysis, 1936 and is represented as follows:

No. of fields in a 1 ml counting mlof cell I mm deep x concentrate the

No. of fields ml of water counted filtered multiplier

The Whipple ocular micrometer was calibrated so that with a lOX ocular any observed field of the counting chamber was exactly equal to one square millimeter. One liter of lake water was concentrated to 10 cc and of the one cc of concentrate placed in the counting cell, ten fields were counted.

Thus: 1000 10

X 10 .1000

RESULTS

Quantitative collections of the phytoplankton of Lake Michigan from November I, 1938 to November I, 1939 yielded genera repre­senting 5 classes of algae. Bacillariophyceae dominated every collec­tion, averaging 94% of the total yield. Chrysophyceae, Myxophy­ceae, and Chlorophyceae were never very abundant. They yielded only 3%, 2 % and 1% respectively while Dinophyceae yielded only a small fraction of I % of the total phytoplankton for the year. The complete data on the occurrence of the various classes of phytoplank­ton are represented in table 1. The environmental factors, sunshine and water temperature, are summarizd in table II.

49

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A maximum yield of 1052 organisms per cc occurred May 9, 1939 while the minimum of 81 cc occurred twice, March 14 and 21, 1939. The maximum and minimum monthly averag'es were established during June and March respectively. Weekly collections of phyto­plankton, .when plotted in gTaph form, gave a very rugged curve and it was difficult to attribute causes to such sudden changes in yield. However, it might be noted that even though the maximum yield per cc occurred in May, the highest monthly average was recorded in June. The g-reatest weekly change in yield occurred be­tween the last two weeks of July when the quantity of phytoplankt?n increased from 223 to 704 plankters per cc. Fragilaria, Tabel1aria, Cyclotella, and Dinobryon were responsible for the sudden increase. Similar increases occurred in April and May but were due mainly to Synedra.

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Page 7: Quantitative study of the phytoplankton of Lake Michigan at … · 2017-05-02 · recorded in June. The g-reatest weekly change in yield occurred be tween the last two weeks of July

TABLE I

Monthly averages of algal classes expressed in numbers per cc and percentage of the total yield of phytoplankton.

Nov. Dec. ]an. Feb. Mar. Apr. May June July Aug. Sept. Oct. Totals Bacillariophyceae-

Percentage ...... , .. , .... 95.9 95.8 93.8 97.1 97.8 98.8 97.5 89.2 90.2 90.3 910 94.0 94 % Per cc ..... - ....... - .... 403 364 272 134 94 266 631 612 388 328 267 366 343

Chrysophyceae-Percentage ............. . 1.5 1.8 3.1 0 0.1 0.4 0.5 4.5 7.0 6.6 5.4 1.4 3 % Per cc .............. , ... 6 7 9 0 1 1 3 31 30 24 16 5 11

t.<l.... Myxophyceae-Percentage . - .... , .. - .... 1.9 18 1.7 2.2 0.1 0.4 0.8 4.2 1.0 1..1 2.0 2.3 2 % Per cc . , ............. , .. 8 7 5 3 1 1 5 29 4 4 6 9 6.8

Chlorophyceae-Percentage ..... , ........ 0.7 06 1.4 0.7 0 0.4 1.2 2.1 1.6 1.7 1.3 2.3 1 % Per cc ... ..... .... 3 2 4 1 0 1 8 15 7 6 4 9 50_ ••• •

Dinophyceae--­Percentage ............... 0 0 0 0 0 0 0 0 0.2 0.3 0.3 0 .06% Per cc ............... - .. 0 0 0 0 0 0 0 0 1 1 1 0 .2

Total per cc ................. 420 380 290 138 96 269 647 687 430 363 294 389 366

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TABLE II

ENVIRONMENTAL FACTORS

MOlllhs Hours of sunshine Average water t.emperature

November, 1938 197.3 Centigrade7.6 0

December, 1938 1I4.3 1Y " January, 1939 85.5 1.2· February, 1939 160.3 0.6· March, 1939 240.7 3.3· April, '1939 211.3 7.5 0

May, 1939 347.7 12,2 0

June, 1939 313.2 17.5 0

July, 1939 358.2 19.7 0

August, 1939 337.7 21.1 0

September, 1939 301.5 18.1 0

October, 1939 233.4 11.4 0

BACILLARIOPHYCEAE

Diatoms are by far the most abundant plankton organisms of Lake Michigan. In April, 98.8% of the total phytoplankton was diatoms while 89.2% was the lowest record for the year and occurred in J ltne when Myxophyceae, Chlorophyceae, and Chrysophyceae were at their maximum. The yearly average for diatoms was 94% of the total phytoplankton. The close parallelism between the yield of di­atoms and the total phytoplankton (fig. I) is broken only in June when the classes mentioned above were at their peaks.

The constituent genera of diatoms and the relative abundance of the domin.ant genera (table III) in numerical order is as follows:

1. Synedra was usually abundant in every collection. It ranged from 65% of the total diatom yield in May to 17% in December. Most abundant in May after which it decreases until September and then established a minor peak in October. The average yield for the year was 391"0 of the total diatoms.

2. Fragilaria was rather abundant throughout the year ranging from 5% in May to 28% in July. The 'average yield for the year was 171"0 of all the diatoms.

3. Tnbellaria occurred rather uniformly throughout the year ranging from 3% in May to 21 % in July. The average yield for the year being 11 % of total diatoms.

32

4. Cyclotella March to 24% in diatoms.

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5. Asterionell in August to 20%1 December and av

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4. Cyclotella was similar to Tabellaria ranging from 3% in March to 24% in April; average yearly yield was 11 '70 of the total

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5. Asterionella was present in all collections ranging from 1% in August to 20% in 1\ovember. It was dominant in November and December and average 7 '70 of total diatoms of the year.

6. NaviCL11a was present in all collections but never abundant. The September yield was 15'70 of the diatoms while the minimum was

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less than 1% in April. The yearly average was 6'10 of the total diatom yield.

7. Melosira occurred the year around. It ranged from less than 1'10 in December to 17% in March with an average of 5% of all the diatoms for the year.

8. Nitzschia was present in nearly all of the collections but never abundant. It averaged 2% of total diatom yield.

9. Rhizosolenia was abundant only in May but was present 111

small quantities during the entire year.

10. Stephanoc\iscu5 was not present in all collections but oc­curred most frequently from May through October.

11. Cymatopleura and Amphiprora were equally abundant oc­cupying .02% of the total diatom yield. The former was present every month with the exception of November and March. The latter was most abundant from May through September.

12. Cymbella and Pinnularia were never abundant and occurred less frequently than the two genera mentioned above.

13. Pleurosigma was reported in December collections but never with a significant number to yield more than .01 % of the total diatoms for that month.

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Page 11: Quantitative study of the phytoplankton of Lake Michigan at … · 2017-05-02 · recorded in June. The g-reatest weekly change in yield occurred be tween the last two weeks of July

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TABLE III Bacillariophyceae-Monthly averages expressed In numbers per cc and percent of the total yield.

No,'. Dec. Jan. Feb. Mar. April May June July Aug. Sept. Oct. Yearly Average

Amphiprora­0.2 001 0.0 0.0 0.0 0.0 0.006 0.6 0.5 0.06 0.07 0.0 0.02 Percent 1. 0.5 0.0 0.0 0.0 0.0 0.4 4.0 2.0 0.2 0.2 0.0 0.7 Per ec

A slerionella­

20.0 19.0 8.4 3.0 5.3 5.4 5.5 2.0 2.0 1.0 2.0 4.0 7.0 Percent 82.0 68.0 23.0 3.6 5.0 14.5 35.0 12.0 8.0 50 ~.O 13.0 22.7 Per cc

Cymhella­

0.2 0.0 0.0 0.01 1.0 0.02 0.003 0003 0.02 0.3 07 0.2 0.01 Percent 10 0.0 00 0.2 1.0 0.7 0.2 0.2 0.7 1.0 2.0 1.0 06 Per cc

Cymalopleura­w U\ 0.0 0.008 0.01 0.04 0.0 0.01 0.3 0.4 0.01 0.01 0.4 0.04 002 Percent

0.0 0.3 0.5 0.6 0.0 0.5 20 3.0 0.5 0.4 1.2 0.2 07 Per cc Cyclotella­

18.0 15.0 11.4 10.0 3.0 24.4 8.0 5.0 7.9 19.0 11.0 6.0 11.0 Percent 73.0 52.0 31.0 14.0 3.0 65.0 50.0 29.0 31.0 620 29.0 22.0 38.4 Per cc

Fragilaria­

16.0 22.0 21.0 14.0 12.0 5.2 5.0 27.0 28.0 14.0 11.0 16.0 ]7.0 Percent 63.0 80.0 58.0 19.0 11.0 14.0 30.0 164.0 109.0 47.0 30.0 58.0 56.9 Per cc

Melosira­

1.4 0.08 1.4 5.0 17.0 15.0 7.0 6.0 2.3 2.0 3.0 5.0 5.0 Percent 6.0 3.0 4.0 7.0 16.0 40.0 43.0 34.0 9.0 6.0 7.0 19.0 16.1 Per cc

l\avicula­6.0 6.0 5.0 8.0 3.0 0.4 2.0 6.0 6.1 6.0 15.0 7.0 6.0 Percent

24.0 22.0 130 10.0 3.0 1.0 13.0 36.0 24.0 19.0 41.0 26.0 19.3 Per cc

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TABLE III-( Continued)

Bacillariophyceae-Monthly average expressed in numbers per cc and percent of the total yield

Nov. Dec. Jan. Feb. Mar. April ~1ay June July Aug. Sept. Oct. Yearly Average

Stephanodiscus­0.0 0.01 0.01 0.0 0.0 0.0 0.4 0.4 0.5 0.6 0.01 0.8 0.3 Percent

0.0 0.7 0.5 0.0 0.0 0.0 30 3.0 2.0 2.0 0.5 3.0 1.2 Per cc

Synedra­22.0 17.0 29.0 45.0 46.0 41.0 65.0 38.0 29.3 34.0 45.0 51.0 39.0 Percent

87.0 62.0 79.0 60.0 43.0 108.0 412.0 234.0 114.0 111.0 121.0 187.0 134.8 Per cc

Tabellaria­14.0 14.0 18.0 11.0 9.0 6.0 3.0 9.0 21.1 20.0 10.0 9.0 11.0 Percent 56.0 51.0 50.0 15.0 8.5 15.0 17.0 57.0 82.0 66.0 26.0 34.0 39.7 Per cc

<-> 0\ Rhizosolenia­

0.2 1.0 2.0 0.04 0.5 0.02 09 3.0 0.2 2.0 0.7 0.1 1.0 Percent 1.0 6.0 5.0 0.6 0.5 0.7 6.0 17.0 1.0 4.4 2.0 0.6 3.7 Per cc

Nitzschia­2.0 5.0 3.0 3.0 3.0 2.2 3.0 3.0 0.8 1.0 0.7 0.2 2.0 Percent 9.0 18.0 8.0 4.0 3.0 6.0 190 18.0 3.0 4.0 2.0 08 7.9 Per cc

Pinnularia­0.0 0.0 0.0 0.0 0.0 0.01 0.008 0.1 0.5 0.0 0.4 0.3 0.001 Percent 0.0 0.0 0.0 0.0 0.0 0.5 0.6 1.0 2.0 0.0 1.0 1.2 0.05 Pcr cc

Pleurosigma­0.0 0.01 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 00 0.0001 Percent 0.0 0.5 00 0.0 0.0 0.0 0.0 00 0.0 0.0 0.0 0.0 0.004 Per cc

Total. .. 403 364 272 134 94 266 631 612 388 328 267 366 343+

I

.., ~ ? ~ ff ~. g- >-l ~ 3 rr, ~ E:.. ~ 0 a '"'1 ~ ('D ~~~§?&&~ o v .... -;:l I" C I"

_. '""' ('D :l ~ 3 ::r ::r o("'l ~::r~l'""'t"~('t)t"D-t ~ g. ~ "0 (',) _. f): ~ .a ~ ('D g g '< o' e; '" .,o C t"D f""'!" '" (1) ~ (1) ~~ ~ e; c. P-. _. ~ '""' ':< "0 " l""'l" (l)o ~ ~ t"D = SlJ ::r::; _. ~ I" ('D ~ '""' ::rc. ::r :: ::l P-. ::r '1"..g Ii> "0 ::r ("'l('D' '""'::r~ < ('D '" ('D ~. ... ~ ~ _. X '"1 (b ~::l::;P:"'?6 ~('D~~ ~lU:3~~

~ S '""' IfQ P3 3-' 0: (') s,,;>, ,... hi (') ~ t:i ev (')~ tJ

('D 9.!,:.;::.:::: - i>::r c . .P> ~ ~ :: ~ ~ ~ ~ ~ ..... O~;;::;-" ...... ~:r ('D ::. n:J,.jUlrbC::::J ........ ",V'J :::J t"D:::J fJ> C1~ &":J V;-::J _. l""+'l '""1: ;:j l'""'t" '"1

~ ('D (C 0 "' ('D ~, '""' '""' ~.g o~q~_r-t-a3tt~ ?''""' P-. ,...,~ ~ ,... :: "Q. ';;; ~ g. ::;. 'Oi.g"'0 ::r(f)""'<~o ""c.,...,o(Jqa"fil ~ ~ ~ --< .g lIJ"~ ~ ~'-'::r ('D 1-000. @ ........C '< ..- ~ ~ ('D ('D' 0 (b';% "g­

:L~~~~r:::5 '<::r n. ~ 0' ::r;:; 0 :::: ::r

- ("'l ~::r ~~l'-l-,"",,<'< ('D [f; "-< ::! C r-f" rt r-+­ i> ~...o ;JI--""< ~::;j ~"< ~ ~ '" ~ (f) -. i> ~ C. '< ("'l rtC~~("';n ::;j-('j:3 r~ ~ ~ O'Q ~ '"' (C1'J,...1"(b....;.~('Di>~o~,.../asc~~~ roT

>(C 0'.10.::"0 '""'('D ("; ...... _. (!! <1>. :[ 0 I" ;::j ~ 'Yv,...~c.""UJ ~=:.g~~~~ %C~ c.:.., ...... lA.9. .-- .. ~iI" G ~, .. A' Q. lA. B. _ "..

Page 13: Quantitative study of the phytoplankton of Lake Michigan at … · 2017-05-02 · recorded in June. The g-reatest weekly change in yield occurred be tween the last two weeks of July

... ::: (J

~ <.J .... l­V (J

0-0­

~g+ oo~

t:')

00

OO~ t:')

0060['-..

~

co 00 00

N <')

00 00 00

00 <'>

00OON ......

'D

00 00-

t:')

'D

00 cio~

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00 00"'"

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00 oo..-s <'> ......

00 dON

"­N

...... 0"" OO~

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o I "'" ol'

~ ~ .;;; , 0

b2 :J

p:;"

CHRYSOPHYCEAE

The Chrysophyceae represented mainly by Dinobryon and Synura was present in all the months of the year with the exception of Feb­ruary. Dinobryon was most abundant from June to October. A major peak was reached in July when 7% of the 1110nthly phytoplank­ton was Chrysophyceae. The average for the year was 3 ro of the to­tal yield of phytoplankton. Observations indicate, on the basis of abundance in plankton tows, that Synura would have been more abundant in the quantitative collections had the colonies not been so easily disassociated in the filtration process.

MYXOPHYCEAE

The rVlyxophyceae were never abundant but were present every month of the year ranging fr0111 0.1 % in March to 4.2% in June. The average for the enti re year was 2 % 0 f the total phytoplankton. These facts are interesting in comparison with the absolute autumnal dominance of blue greens in Lake Erie (Tiffany, 1938). It is pos­sible that the greater depth of Lake NIichigan and a lower maximum temperature do not permit such an autumnal development of blue greens. The filamentous forms were represented by Oscillatoria, Anabaena, and Lyngbya while the colonial members were mainly, Microcystis, Merismopedia, Chroococcus, and Coelosphaerium.

CHLOROPHYCEAE

The Chlorophyceae like the blue greens were never abundant but were presentJ every month with the exception of March. In June, the maximuDl yield was reached with 15 organisms per cc which was the equivalent to 2ro of the total monthly yield of phytoplankton. In July I Aug'ust, September, and October, the greens were more abund­ant than in the winter and spring months. The class was repre­sented mainly by Scenedesmus, Ankistrodesmus, Coelastrum, Pedi­astrum, Dictyosphaerium and Westella. The Chlorophyceae were responsible for lro of the total yield of phytoplankton for the year.

DINOPHYCEAE

The Dinophyceae yield was never abundant and the only monthly records were July, August, and September. Ceratium was present

37

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1938 with the pres ber, 1938 to ~ovc

in July, and Peridinium in August and September. However, [rom the observations on plankton tows, it appeared that Ceratium was more abundant and present over a longer period of time than the quantitative collections revealrd. The class was responsible for .06% of the total phytoplankton for the year.

COMPARISON OF THE 1937-38 A\JD THE 1938-39 DATA

In comparing the results of the quantitative study of the phyto­plankton of Lake Michigan made by Daily from May, 1937 to May,

FIGURE 2

2.054

193"'7 - 1938 J\Ane - Ju.n.e

1936 - 1939 Nov - NOli.

~

-I.i \J

~ ()

~ ~

~

~

~

38

tained. However presents a problem

Regardless 0 i tn phytoplankton, man' itely confimled wit the classes both re the sanle order of lariophyceae the r i\.lyophyceae, Chlon plankton showed spring and autumn maxima in monthl cUHed in March. and species at all ti ture and hours of s iodicity. The gcner. were essentially th of Phytoplankton c<

The consistcntl prompted an invcs prohable that 1938-l as the yield of phytl tors, chemical and as to prevent a yie character of the gr but it also appears well be operating counting error was throughout the stu major inconsistenci are to be consider method of determi followed. He stat regardless of the n Rhizosolenia, Scen Tabelaria, and Mel wise, a single cell

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from ,1 was

.",n the .06~!o

phyto­J May,

1938 with the present study which involved collections from N ovem­ber, 1938 to November, 1939, a close parallelism (fig. 2) was ob­tained. However, the consistently lower yield during 1938-39 presents a problem which allows for much speculation .

Regardless of the seemingly great difference in total yield of the phytoplankton, many of the conclusions drawn by Daily were defin­itely con firmed with the 1938-39 data. Tbe. total phytoplankton and the classes both revealed marked periodicity. The classes occupied the same order of numerical abundance (table I) with the Bacil­lariophyceae the most abundant followed by the Chrysophyceae, Myophyceae, Chlorophyceae, and Dinophyceae. The total phyto­plankton showed considerable weekly variation and it exhibited a

I spring and autumn maximum. Both the 1937-38 and the 1938-39

t maxima in monthly yields occurred in June while the minima oc­curred in March. The order Pennales led the Centrales in number

I and species at all times. The environmental factors, water tempera­ture and hours of sunshine again showed a definite correlation to per­( iodicity. The genera and species occurring during the 1938-39 study were essentially the same as those included in the Systematic List of Phytoplankton compiled by Daily.

The consistently lower yield for the entire year, 1938-39, prompted an investigation as to its possibilities. First, it is highly probable that 1938-39 could have been a "deficiency" year as far as the yield of phytoplankton is concerned. The environmental fac­tors, chemical and physical, could have been operating in such a way as to prevent a yield comparable to that reported in 1937-38. The character of the graph (fig. 2) would tend to substantiate this claim but it also appears obvious that a consistent error in method might well be operating to produce such a relationship. However, if a counting error was operating, it would, ont of necessity, be consistent throughout the study and could not be used in explaining the two major inconsistencies in yield occurring in June and August which are to be considered later. Close check was made with Daily's method of determining counting units and the same procedure was followed. He states: "In counting the organisms, each planktont, regardless of the number of component cells, was counted as a unit. Rhizosolenia, Scenedesmus, Dinobryon, filaments of Fragilaria, Tabelaria, and Melosira were respectively counted as units. Like­wise, a single cell of a colony or filament was counted as a unit if

39

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found separated from the original aggregate. As suggested by Todd and Sanford for blood corpuscle enumeration, cells which touched the lower and right sides of each square were counted as if within the squares."

As the possibility of an error 111 filtration and concentration ap­peared likely, water samples were taken over a period of fifteen con­seclltive days and concentrated in different ways by varying the amount of suction pressure to determine the effect it might bave on numerical counts. Surprisingly, the amount of suction had little or no effect on the quantitative counts. Over a five-day period there were 7 more plankters per cc in the sample concentrated with a slow rate of suction. Even though the amount of suction apparcntly had little or no effect on the number of plankters per cc, it did not neces­sarily follow that organisms were not being lost by the use of suction in the process of concentration. Further study revealed that slightly more organisms were present when concentrated by the use of gravity only for pressme but the significant diHerence occurred when counts were made on an original cc of unconcentrated lake water and com­pared with a count derived after the lake water had been subjected to, concentration by filtering through sand. A concentrated cc con­tained 314 p1ankters while the original unconcentrated cc of lake water contained 1452 plankters as an average per day over a five day period. However, more comparative counts will have to be made over a longer period of time before we can state the percentage of plankters lost by concentration.

Besides the low yield of 1938-39 another problem involves the explanation of the extremely high peak established in June, 1937-38 and the absence of such a peak in 1938-39, A sudden increase in yield during August was also evidenced in 1937-38 while in 1938-39, the yield for the same month showed a gradual decline over that of the preceding months. .-\s the Bacillariophyceae was responsible for 9410 of the total phytoplankton yield, it was by analyzing the yields of the various genera of diatoms that a partial explan3 tion of the two conspicuous inconsistencies was derived. First, the exceptionally high increase of Syneclra (fig. 3) from May to June of 1937-38 and a marked decline during June 1938-39 explain the largest variation in yield, A second but lesser peak of Synedra in August 1937-38 and no such occurrence in 1938-39 explain the other. The February, 1937-38 yield of Synedra failed to alter the consistent character of

40

the total phytoplankton emv during November, Decembe set such a possibility. Fro that each genus has high Clt

\.. 100'

19~1 - 1938

•11 Ju.ne - Ju.ne

1938 - 1939

Nov. - Nov.

~ ~ 900

~ .~ 600 ~

~lO \.. a 600

.....(:) 50.0

~ \l 400 ~.

t ~ 300

~ '200

100

o

anyone year. It is probah the hig-her total yield of p to Syncdra, Bayliss <;illd C

have a pronounced ef feet

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tion ap­:cen con­ying the have on little or

tod there th a slow 'cntly had .1ot neces­l suction

~t slightly o£ gravity ;:ten counts and com­subjected d cc con-

cc of lake vcr a five ,~Ive to be percentage

llvolves the e, 1937-38 increase in in 1938-39, ver that of

nsible for fg the yields tion of the

~r'cePtional1Y 1937-38 and variation in

1937-38 and te February, eharacter of

16"l8

41

"~ II 1931 - \938

Ju.ne - Ju.ne

12 • \93B - 1939

Nov. - Nov.

\00

F1GURE 3

o

have a pronounced effcct upon the total yield of phytoplankton for anyone year, It is probable thcn, that such evidence would support the higher total yield of phytoplankton for 1937-38 as due mainly to Synedra. Bayliss and Cerstein (2) have records bearing out thts

the total phytoplankton curve (fig. 2). The abundance of Fragilaria during November, December, and January preceding February off­set such a possibility. From the evidence, now at hand, it appears that each genus has high and low productive years which in turn

7.00

~ 100' ~

~ ~ 900

~ .~ 800

~10 ~ o 600

'i... I;) 500

~ \l 400 ~,

~ ~ 300

<

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assumption during 1927 and 1928 when Tabellaria was especially abllOdant during the first year and then the second followed as a very low productive year for the genus Tabellaria mainly. It would ap­pear (fig. 3) that the Synedra yield of 1938-39 should have sur­passed the 1937-38 yield according to its abundance during April and May. However, it is evident that something happened and in­stead of an expected increase in June there was a marked decline of Synedra. It is with the contributing factors to such a variation in yield that our problem is now centered.

SUMMARY AND COKCLUSIONS

I. The Bacillariophyceae dominated every collection, averaging 94 % of the total phytoplankton. Chrysophyceae. Myxophyceae, and Chlorophyceae were responsible for 3%, 2% and 170 respectively, while Dinophyceae yielded only a small fraction of 170 (table I).

2. The maximum weekly yield of 1052 organisms per cubic centimeter was recorded Iv1ay 9, 1939 while the minimum of 81 oc­curred twice, March 14 anel 21, 1939. The maximum and minimum monthly totals (fig. I) were established during June and March respectively.

3. Two peaks of ahundance were evident in the average monthly totals of phytoplankton. The major peak occurred in June; the minor in November.

4. Each genus displayed its own pulse, independently at some­time throughout the year. Asterionel1a and Cydotella each reached a peak in November, Synedra in May, Fragilaria in June and Tabel­laria in July.

5. The total Bacillariophyceae closely parallels the total phyto­plankton except in June, when the Chrysophyceae, Myxophyceae, and Chlorophyceae reached their peaks (fig. 1).

6. Some of the constituent genera of diatoms and their relative abundance for the year are the following: Synedra, 3970; Fragil­aria, 17%; Tabellaria, 11 %; Cyclotella, 11 % ; Asterionella, 7%; 1\'avicula, 6%; Melosira, 5%; Nitzschia, 2%; Rhizosolenia, 1% ; Stephanodiscus, 0.3%, Cymatopleura, Amphiprora, Cymbella, Pin­nl1laria and Pleurosigma ranked in the order mentioned but with an almost insignificant yield.

42

7. The Ch Syoura were pTI

Feb rtta ry. The phytoplankton.

8. The Myx month of the y' The average for Tiffany (1938) Myxophyceae in tected in I.ake 1'1

9. The ChlQ present every m sponsible for 1%

10. The Dil during three COl and T'cridinium . tow collections "e more ahundant t

11. Temper total phytoplank" periodicity.

]2. I'ours (J

phytoplankton d or no relation to

13. A close 1938-:~9 ql1antita However, a corn and the total yiel of the conspicu(1,ll totals of phy(opl, explanati on 0 f might be respon various genera aI

for the variation"

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especially as a very lOuld ap­

have Sllr­

'ng April .. .d and in­decline of riation in

'" averagmg

yceae, and spectively, ,Ie I).

per cubic of 81 oc­minimum

nd March

~e monthly June; the

y at some­ch reached and Tabel­

otal phyto­yxophyceae,

heir relative 0/0; Fragil­

onella, 7%; ~Ienia, I ero ; bnbella, Pin-but with an

7. The Chyrsophyceae represented mainly by Dinobryon and Synura werc present in all monthly averages with the exception of February. The average for the year was 3% of the total phytoplankton.

8. The Myxophyceae were never abundant but were present every month of the year; ranging from 0.1 % in March to 4.2% in June. The average for the entire year was 2% of the total phytoplankton. Tiffany (1938) reported an absolute autumnal dominance of the Myxophyceae in Lake Erie but no such occurrence has yet been de­tected in Lake Michigan.

9. The Chlorophyceae, likewise, were never abundant but werc present every month with the exception of March. They were re­sponsible for I % of the total phytoplankton.

10. The Dinophyceae, few in number and species, were present during three consecutive months only. Ceratium occurred in July and Peridinium in August ancl September. However, the plankton tow collections seem to indicate that the above mcntioned forms were more abundant throughout the year.

11. Temperature apparently has a pronounced effect upon the total phytoplankton but is not the sole determining factor of periodicity.

12. Hours of sunshine show a positive correlation with the total phytoplankton during the spring and summer months but have little or no relation to the October increase.

13. A close parallelism was evident between the 1937-38and the: 1938-39 quantitative studies of the phytoplankton of Lake Michig"an. However, a comparison of the total yield of phytoplankton (fig. 2) and the total yield of Synedra (fig. 3) reveals a partial explanation of the conspicuous inconsistencies, the 1937-38 J line and August totals of phytoplankton. As a result, it hecomes evident that the real explanation of the problem now lies in determining what factors mig'ht be responsible for the high ane! low productive years of the various genera and not alone determining what genera are responsible for the variations in yield f rom year to year.

43

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ACKr.:O\VLEDGEMENTS

The writer wishes to express his appreCiatIOn to Dr. Hanford Tiffany of Northwestern University for his assistance given through­out the study and preparation of the paper. Indebtedness is also due to "\V. A. Daily of the l_~niversity of Cincinnati for making bis 1937-38 data available.

LITERATURE CITED

1. AMEIHCAN PCBI.JC HE.\I.TH ASSOCLATlON. Standard methods of water analysis. Seventh Ed. New York. 1933.

2. BAYLIS~,]. R. and GERsnIN, H. M. Micro-organisms in the lake water at Chicago. Municipal ~ews and Water Works. Pp.291-296. 1929.

3. BIRGE, E. A. and JUD.W, CH,'INCEY. The inland lakes of Wisconsin. The plankton. its quantity and chemical composition. Wis. Geol. Nat. Hist. Surv. Bull. 64., Ser. 13. 1922.

4. TIRJGGS, S. A. The diatomaceae of Lake Michigan. The Lens. 1 :41-44. 1872.

5. COFFING, CHARLENE. A qualltitative study of the phytoplankton of the White Ii.iver Canal, Indianapolis, Indiana. Butler Univ. Bot. Stud. 4 :13-31. 1937.

6. DAILY, W. A. A quantitative study of the phytoplankton of Lake Michigan collected in the vicinity of Evanston, Illinois. Butler Univ. Bot. Stud. 4 :65-38. 1938.

7. EDDY, SAMUEL. The plankton of Lake Michigan. Illinois State Nat. Hist. Surv. Bull. 17 :203-232. 1927.

8. ELM OIlE, c.]. The diatums (Bacillarioideae) of Nebraska. A thesis in partial fulfillment of the requirements for the degree of Doctor of Philosophy. Lincoln, Nebraska. 1921.

9. LILLTCK, L. C. Seasonal studies of the phytoplankton off Woods Hole, Massachusetts. BioI. Bull. 73 :488-503. 1937.

10. PEARSALL, W. H. A theory of diatom periodicity. Jour. &01. 11 :165-183. 1923.

11. Sl>llTli, G. M. The freshwater algae of the United States. McGraw-Hill Book Company. New York. 1933.

12. TIFFANY, L. H. Plankton algae of the west end of Lake Erie. Ohio State University Press. Contr. No.6. 1934.

13. -- The filamentous algae of the west end of Lake Erie. Am. Midland Nat. 18 :911-951. 1938.

I·t -- Algae: the grass of many waters. (has. C. Thomas Publisher. 1938.

15. TRANSE/\U, E. N. The periodicity of freshwater algae. Am. Jour. Bot. 3 :121-133. 1916.

16. WELCH, PAUL S. Limnology. McGraw-Hili Book Company. New York. 1935.

44

THE DIREc aVERA CREAME

This study il scopic count of intervals, over a the morphologic" determine the p the seasonal diff of the three sour

Sedgwick and cal examination to the public real

• beginnings of ce involved the lise of bacteria pre which first uncle supply. ProhabJ organized by Co American Pnhli ardize methods Q

up and adopted • had adopted pia· and Boston area sediments for let Standard Metho than 294,000 s United States a

The direct proved to he of i

is the best mean of body cells in temlining the ty infected milk; formers, colOI1