quantitation part 9 by : saib al owini. cell counting - hemocytometer -electronic counting
TRANSCRIPT
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Quantitationpart 9
By : Saib Al Owini
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• CELL COUNTING
- Hemocytometer
-Electronic Counting
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PROTOCOL 21.1. CELL COUNTING BYHEMOCYTOMETER
OutlineTrypsinize a monolayer culture, or sample a suspension culture, prepare a
hemocytometer slide, and add the cells to the counting chamber. Count the cells on a microscope and calculate the cell concentration.
• Materials• Sterile:• D-PBSA• Crude trypsin, 0.25%• Growth medium• Yellow pipettor tips• Nonsterile:• Pipettor, 20 μL or adjustable 100 μL• Hemocytometer (Improved Neubauer)• Tally counter• Microscope
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• H:\ ملفات YouTube - hemacytometer count- sabrina lin.flv\فيديوأنسجة
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Electronic Counting
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CASY Electronic Cell Counter.
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CASY Electronic Cell Counter.
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Beckman Coulter Vi-CELL Electronic Cell Counter
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CELL PROLIFERATION
• Experimental Design
• Growth Cycle
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Cytotoxicity
• Viability
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•H:\ ملفات YouTube - Trypan Blue part 3.flv\فيديوأنسجة•H:\ ملفات YouTube - Trypan Blue part 2.flv\فيديوأنسجة•H:\ ملفات YouTube - Trypan Blue part 1.flv\فيديوأنسجة
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Metabolic Cytotoxicity Assays
• Microtitration Assays
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