quantify active psa in vivo · psa 750 fast™ is a unique type of activatable in vivo fluorescent...
TRANSCRIPT
PSA is one of the most studied biomarkers for Prostate Cancer. However, serum PSA levels are mildly elevated in both malignant and non-malignant prostate conditions. Enzymatically active PSA, present at detectable levels only in prostate tumor tissue, provides a better target to measure the malignancy of prostate cancer.
PSA 750 FAST™ is a unique type of activatable in vivo fluorescent agent that detects and quantifies PSA enzymatic activity at sites of prostate tumor growth.
• Detect and quantify active PSA non-invasively in vivo
• Selective against unbound and complexed PSA
• High Specificity for PSA+ tumors
• Validated on PerkinElmer’s comprehensive line of in vivo imaging systems
PSA 750 FASTActivatable Fluorescent Pre-clinical Imaging Agent
QUANTIFY ACTIVE PSAIN VIVO
PSA 750 FAST™ Mechanism of Action
PSA 750 FAST™ has been validated on both the IVIS and the FMT platforms. Images above show clear targeting to PSA+ LNCaP tumors. Mice were injected with 2 nmoles of PSA agent and imaged 24 hours post injection.
IVIS Spectrum FMT 2D FMT 3D
Quenched
Fluorescent Fluorescent
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010476_01 Printed in USA August 2012
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(A) - In vivo imaging in PSA+ and PSA- tumors after injection of PSA 750 FAST™. Tumor-bearing male Nu/Nu injected i.v. with 2 nmoles of agent and imaged 24 hours later. Four mice were implanted with either LNCaP (PSA+) or PC-3 (PSA-) cells. Images show concentration of the agent (pmoles) in tumor bearing mice. (B) - Chart on the top shows tumor fluorescence significantly higher in PSA+ LNCaP than in PSA- PC-3 tumors in vivo. Bottom graph shows a very good correlation between tumor signal and tumor size (C) - Enzyme biochemistry validation showing PSA 750 FAST™ is specifically cleaved by active PSA over inactive PSA (complexed with its inhibitor a1-antichymotrypsin), and other disease-relevant enzymes. (D) - Ex vivo validation of the specificity of PSA 750 FAST™ by microscopy. Complete blockage of fluorescent signal observed after pre-incubating tumor sections with the PSA natural inhibitor, a1-antichymotrypsin.
A
C
B
D
PSA+ LNCaP
5.5 pmol
16.8 pmol
58.9 pmol1.9 pmol
31.2 pmol
5.8 pmol6.6 pmol
2.7 pmol
PSA- PC-3
Learn more at www.perkinelmer.com/invivoreagents
PerkinElmer in vivo imaging reagents are intended for animal research and not for use in humans.
Validated and optimized for use on all PerkinElmer's in vivo imaging systems
IVIS® Spectrum CT FMT® 4000 Maestro™ 2 Quantum FX μCT