Quantification of Oil Present Quantification of Oil Present in Corn Masa By-product in Corn Masa By-product

StreamStream

Amy Dee SchlechteAmy Dee Schlechte

Maitri ThakurMaitri Thakur

AgendaAgenda

Introduction to Corn MasaIntroduction to Corn Masa Project ObjectivesProject Objectives ProcedureProcedure Results and DiscussionsResults and Discussions ConclusionConclusion Future RecommendationsFuture Recommendations Questions Questions

Introduction to Corn MasaIntroduction to Corn Masa

Corn is a Valuable CommodityCorn is a Valuable Commodity Starch, Cereal, Tortillas, etc….Starch, Cereal, Tortillas, etc….

Corn Masa Processing (Nixtamalization)Corn Masa Processing (Nixtamalization) Grain Cooked with HGrain Cooked with H220 & Lime0 & Lime SteepedSteeped SeparatedSeparated

Cooked Grain Cooked Grain Steep LiquorSteep Liquor

IntroductionIntroduction

Nutrient Loss in Steeping LiquidNutrient Loss in Steeping Liquid Fiber, Ash, Minerals, Proteins, OilFiber, Ash, Minerals, Proteins, Oil

Oil Extraction MethodsOil Extraction Methods Solvent Extraction using HexaneSolvent Extraction using Hexane Supercritical Fluid Extraction (SFE) using Supercritical Fluid Extraction (SFE) using

COCO22

ObjectivesObjectives

Find a Suitable Method for Oil ExtractionFind a Suitable Method for Oil Extraction

Compare Solvent Extraction & Super Compare Solvent Extraction & Super Critical Fluid Extraction MethodsCritical Fluid Extraction Methods

Determine if Oil is Concentrated in High Determine if Oil is Concentrated in High or Low Density Fraction of By-productor Low Density Fraction of By-product

ProcedureProcedure

Obtained 2 Samples - Minsa Obtained 2 Samples - Minsa Red Oak, Iowa Red Oak, Iowa

Dried Samples @ 50Dried Samples @ 50oo C for 7 Days C for 7 Days

Performed Solvent Extraction & SFE Performed Solvent Extraction & SFE

Solvent Extraction Solvent Extraction ProcedureProcedure

Combined 25g Dried Masa w/ Combined 25g Dried Masa w/ 75 ml Hexane75 ml Hexane

Filtered & Replaced Solvent 3 Filtered & Replaced Solvent 3 Times/24 HrsTimes/24 Hrs

Accumulated 1200ml of Accumulated 1200ml of Solvent Solvent

Used Rotary Evaporator to Used Rotary Evaporator to Reclaim Oil from SolventReclaim Oil from Solvent

SFE ProcedureSFE Procedure

Sample 2 Centrifuged @ 4400 RPM Sample 2 Centrifuged @ 4400 RPM for 20 Minutesfor 20 Minutes High Density FractionHigh Density Fraction Low Density FractionLow Density Fraction

SFE Performed using COSFE Performed using CO22 5 Samples (2-3g each)5 Samples (2-3g each) 2 Reps @ 42 min/cycle2 Reps @ 42 min/cycle

SFE ProcessSFE Process

Supercritical conditions 8500 psi and Supercritical conditions 8500 psi and 12012000 C C

Results & DiscussionResults & DiscussionHexane Extraction of Oil

0.05%

0.07%

0

0.01

0.02

0.03

0.04

0.05

0.06

0.07

0.08

1 2

Sample

Perc

en

tag

e o

f O

il (

%)

Total oilextracted (%)

Results & DiscussionResults & Discussion

Hexane ExtractionHexane Extraction Low Percentage Oil Collected Low Percentage Oil Collected Consistent ResultsConsistent Results

Test ImplicationsTest Implications Oil Still ContainedOil Still Contained Low Oil ContentLow Oil Content

Results & DiscussionResults & DiscussionSupercritical Extraction of Oil

0.33%

0.41%0.45%

0.28%

0.72%

0.00

0.10

0.20

0.30

0.40

0.50

0.60

0.70

0.80P

erc

enta

ge o

f O

il (%

)

Masa 1 High Density Low Density Masa 2 Hex Ext.

Results & DiscussionResults & Discussion

SFESFE Consistent Results From Duplicate Tests Consistent Results From Duplicate Tests Greater Yield than Solvent ExtractionGreater Yield than Solvent Extraction

Test ImplicationsTest Implications Solvent Extraction + SFE = Higher Extractable Solvent Extraction + SFE = Higher Extractable

OilOil Consistent Results Consistent Results

High & Low Density Samples High & Low Density Samples Masa 1 & Masa 2Masa 1 & Masa 2

ConclusionsConclusions

Low Overall Oil Content Low Overall Oil Content

SFE vs. Solvent ExtractionSFE vs. Solvent Extraction

Synergistic EffectsSynergistic Effects

Equal Distribution of OilEqual Distribution of Oil

Future RecommendationsFuture Recommendations

Use a Different Solvent (Methanol & Use a Different Solvent (Methanol & Chloroform)Chloroform)

Perform Solvent Extraction with Perform Solvent Extraction with different ratios of Sample to Solventdifferent ratios of Sample to Solvent

Perform Additional Tests With High Perform Additional Tests With High and Low Density Fractionsand Low Density Fractions

Questions??Questions??