30

BiopharmaLynx 1.3

8 105

32

UPLC®/MS

E

BiopharmaLynxTM

MSE

Trastuzumab

ACQUITY UPLC® BEH300 C18 1.7µm

2.1*150mm

Synapt® G2 HDMS

Endoproteinase Lys-C Iodoacetamide

RapiGestTM

Waters p/n 186001861

Lys-C

RapiGest

LC/MSE

BiopharmaLynx Trastuzumab

1 1

1:K1-1:K4

1 1:K1-1:K4

10657.12Da MH+

1C MSE

MSE

1:K1 1:K4

b/y

7019.27Da 1/y39-2/y24 10431.19Da 1/y39-2/

y56

1. 1:K1-1:K4 A UPLC/MS B

C BiopharmaLynx D MSE

31

1:K14-1:K13 SFNRGEC=SCDK

BiopharmaLynx

2:K14-4:K14

1

BiopharmaLynx

Scrambled

Disuldes

1:K13-2:K16 1:K14-2:K16

1:K7-2:K21

1:K7-2:K21

3A

Synapt G2

3B

1. 1:K1-1:K4 A UPLC/MS B

C BiopharmaLynx D MSE

3. A)BiopharmaLynx 1:K13-2:K16,1:K7-2:K21,1:K14-2:K16 B 1:K13-2:K16

2.

!

(UPLC/MSE

BiopharmaLynx1.3 )

Lys-C