purpose : stocking density, walleye survival, and zooplankton communities at baldhill national fish...

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Purpose: Stocking Density, Walleye Survival, and Zooplankton Communities at Baldhill National Fish Hatchery Amy Doll 1 , Casey Williams 1 , Kurt Eversman 2 , David Demuth 3 ; 1 Department of Science, Valley City State University, Valley City, ND 58072; 2 US Fish and Wildlife Service Valley City National Fish Hatchery, Valley City, ND 58072, 3 Great Plains STEM Education and Research Center, Valley City State University, Valley City, ND 58072 Methods: Results: Walleye Fry were stocked in six ponds (Figure 1) at three stocking rates on June 3, 2013 200,000 fish/surface acre (FPSA) – Ponds 2 and 5 250,000 fish/surface acre – Ponds 6 and 9 300,000 fish/surface acre – Ponds 13 and 14 Harvested between June 24 th and June 26th Percent survival of walleye stocking densities were arcsine transformed and analyzed with 1 Factor Analysis of Variance (ANOVA; α = 0.05). Tukey’s test (α = 0.05) were used for pairwise comparisons. Ponds were normally fertilized twice a week. Zooplankton Sampled on Mondays and Thursdays during June. Split into manageable sample size using a Folsom plankton splitter Counted and identified using a Ward Counting Wheel. Categorized into five groups: rotifers, cyclopoids, calanoids, nauplii, and cladocerans This study was conducted to examine juvenile walleye Sander vitreus fry survivorship at three stocking densities in ponds at Baldhill National Fish Hatchery. Walleye survival rates were correlated with temporal patterns in zooplankton populations to examine possible interspecific regulation of walleye abundance and survival. Compared to higher stocking rates, stocking walleye fry at 200,000 fish per surface acre results in the highest survivorship; thus, the lower stocking density should be used to maximize walleye production at Baldhill National Fish Hatchery (Figure 4). Current fertilization rates appear to promote adequate zooplankton communities to support walleye production at Baldhill National Fish Hatchery. Figure 3. Temporal plankton density in Baldhill National Fish Hatchery ponds stocked with walleye fry at three stocking densities. Figure 1. Aerial view of Baldhill National Fish Hatchery 1 4 8 12 2 3 5 9 13 6 15 16 7 10 11 14 17 18 19 20 Figure 2. Percent survivorship for three stocking rates of walleye fry at Baldhill National Fish Hatchery. Letter designations (A, B) represent statistical significance (α = 0.05) between stocking rates. R otifers D ensity (#/Liter) 0 100 200 300 400 C yclopods 0 2 4 6 8 10 12 14 16 Calanoids 0.0 0.5 1.0 1.5 2.0 C ladocerans Sam pling D ate 6/3 6/6 6/10 6/13 6/17 6/19 0 5 10 15 Pond 2 Pond 5 Pond 6 Pond 9 Pond 13 Pond 14 } 200,000 FPSA } 250,000 FPSA } 300,000 FPSA Stocking R ate (#/surface acre in thousands) 200 250 300 PercentSurvivorship (% ) 10 20 30 40 50 A AB B Conclusion: Walleye percent survivorship was significantly higher (P < 0.05) when stocked at 200,000 FPSA (mean = 47.5%, SD =0.7) than 300,000 FPSA (mean = 25.5%, SD =6.4; Figure 2). Although not statistically significant (P >0.05), walleye survivorship was higher when stocked at 200,000 FPSA than when stocked at 250,000 FPSA (mean = 33%, SD = 1.4). Similar trends in zooplankton abundance across ponds suggests zooplankton abundance is not a limiting factor between different stocking rates (Figure 3). Stocking D ensity (in thousands) 200 250 300 # Fish Produced (in thousands) 70 80 90 Figure 4. Mean number of fish produced per surface acre for three stocking densities at Baldhill National Fish Hatchery.

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Page 1: Purpose : Stocking Density, Walleye Survival, and Zooplankton Communities at Baldhill National Fish Hatchery Amy Doll 1, Casey Williams 1, Kurt Eversman

Purpose:

Stocking Density, Walleye Survival, and Zooplankton Communities at Baldhill National Fish HatcheryAmy Doll 1, Casey Williams 1, Kurt Eversman 2, David Demuth3; 1Department of Science, Valley City State University, Valley City, ND 58072; 2US Fish and Wildlife Service Valley City National Fish Hatchery, Valley City, ND 58072, 3 Great

Plains STEM Education and Research Center, Valley City State University, Valley City, ND 58072

Methods:

Results:

WalleyeFry were stocked in six ponds (Figure 1) at three

stocking rates on June 3, 2013200,000 fish/surface acre (FPSA) – Ponds 2 and 5250,000 fish/surface acre – Ponds 6 and 9300,000 fish/surface acre – Ponds 13 and 14

Harvested between June 24th and June 26thPercent survival of walleye stocking densities were

arcsine transformed and analyzed with 1 Factor Analysis of Variance (ANOVA; α = 0.05). Tukey’s test (α = 0.05) were used for pairwise comparisons.

Ponds were normally fertilized twice a week.Zooplankton

Sampled on Mondays and Thursdays during June.Split into manageable sample size using a Folsom

plankton splitterCounted and identified using a Ward Counting

Wheel. Categorized into five groups: rotifers, cyclopoids,

calanoids, nauplii, and cladocerans

This study was conducted to examine juvenile walleye Sander vitreus fry survivorship at three stocking densities in ponds at Baldhill National Fish Hatchery.

Walleye survival rates were correlated with temporal patterns in zooplankton populations to examine possible interspecific regulation of walleye abundance and survival.

Compared to higher stocking rates, stocking walleye fry at 200,000 fish per surface acre results in the highest survivorship; thus, the lower stocking density should be used to maximize walleye production at Baldhill National Fish Hatchery (Figure 4).

Current fertilization rates appear to promote adequate zooplankton communities to support walleye production at Baldhill National Fish Hatchery.

Figure 3. Temporal plankton density in Baldhill National Fish Hatchery ponds stocked with walleye fry at three stocking densities.Figure 1. Aerial view of Baldhill National Fish Hatchery

1

4

8

12

23

5

9

13

6

15

16

710

1114 17

18

1920

Figure 2. Percent survivorship for three stocking rates of walleye fry at Baldhill National Fish Hatchery. Letter designations (A, B) represent statistical significance (α = 0.05) between stocking rates.

Rotifers

X Data

8 16 53 106 53 99

Den

sity

(#/

Lite

r)

0

100

200

300

400

Cyclopods

X Data

8 16 53 106 53 99

02468

10121416

Calanoids

X Data

8 16 53 106 53 99

0.0

0.5

1.0

1.5

2.0

Cladocerans

Sampling Date

6/3 6/6 6/10 6/13 6/17 6/19

0

5

10

15

Pond 2Pond 5Pond 6Pond 9Pond 13Pond 14

} 200,000 FPSA

} 250,000 FPSA

} 300,000 FPSA

Stocking Rate (#/surface acre in thousands)200 250 300

Per

cent

Sur

vivo

rshi

p (%

)

10

20

30

40

50 A

ABB

Conclusion:

Walleye percent survivorship was significantly higher (P < 0.05) when stocked at 200,000 FPSA (mean = 47.5%, SD =0.7) than 300,000 FPSA (mean = 25.5%, SD =6.4; Figure 2).

Although not statistically significant (P >0.05), walleye survivorship was higher when stocked at 200,000 FPSA than when stocked at 250,000 FPSA (mean = 33%, SD = 1.4).

Similar trends in zooplankton abundance across ponds suggests zooplankton abundance is not a limiting factor between different stocking rates (Figure 3).

Stocking Density (in thousands)

200 250 300

# F

ish

Pro

duce

d (in

tho

usan

ds)

70

80

90

Figure 4. Mean number of fish produced per surface acre for three stocking densities at Baldhill National Fish Hatchery.