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TRANSCRIPT
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∼
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∼
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∼
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△ ▲ ◑ ◮
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Germicidal effectiveness curve
200 280 320 400 Wavelength [nm]
UV-C UV-B UV-A Vacuum-UV
10
GGG
0 254nm
Mercury low-pressure lamp
infrared gamma rays ultraviolet
10-8m 10-3m
X-rays
10-11m
microwaves
visible light 400 - 780nm
BB UV-AUV A
10-6m
-
Stepped
Type T thermocouple
Polystyrene test tube
Human milk
-
R² = 0.976 R² = 0.99776
0
12
24
36
48
60
72
1.E+00
1.E+01
1.E+02
1.E+03
1.E+04
1.E+05
1.E+06
0 20 40 60 80 100 120
Tem
pera
ture
[°C
]
E. c
oli c
ount
[CFU
/ml]
Time [s]
-
R² = 0.99776
0
7
14
21
28
35
42
49
56
63
70
0
5,000
10,000
15,000
20,000
25,000
30,000
35,000
40,000
45,000
50,000
0 20 40 60 80 100 120
Tem
pera
ture
[°C
]
BSS
L ac
tivity
[U/l]
Time [s]
-
0
6
12
18
24
30
36
42
48
54
60
0
25,000
50,000
75,000
100,000
125,000
150,000
175,000
200,000
225,000
250,000
untreated 276s at 3·62W
114·5s at 8·73W
70·5s at 14·18W
50·5s at 19·8W
39s at 25·64W
Tem
pera
ture
[°C
]
E. c
oli c
ount
[CFU
/ml]
BSS
L ac
tivity
[U/l]
-
⎯ ⎯
-
10.4
87
1.71
3
-
0
40000
80000
120000
160000
200000
0 5 10 15 20 25 30 35 40 45
Fluo
resc
ence
H2O2 concentration [µM]
PBS Holder pasteurized human milk Human milk PBS Holder pasteurized human milk Untreated human milk
-
0
2000
4000
6000
8000
10000
12000
14000
16000
18000
0 10 20 30 40
Fluo
resc
ence
Reading time after adding reagents [min]
PBS Holder pasteurized human milk
Untreated human milk NaH2PO4
NaCl KCl
DDI
-
0
5000
10000
15000
20000
25000
-3 0 3 6 9 12 15 18 21 24 27 30 33 36 39 42 45
Fluo
resc
ence
Time [min]
Sample 1
Sample 2
Sample 3
Sample 4
-
4000
2000
6000
8000
0
Fluo
resc
ence
Unt
reat
ed h
uman
milk
UV-
C ir
radi
ated
hum
an m
ilk
Hol
der p
aste
uriz
ed h
uman
milk
untre
ated
hum
an m
ilk
untre
ated
hum
an m
ilk +
cat
alas
e (1
U/m
l)
untre
ated
hum
an m
ilk +
H2O
2 (10
µM
) UV
-C h
uman
milk
UV-C
hum
an m
ilk +
cat
alas
e (1
U/m
l)
UV-C
hum
an m
ilk +
H2O
2 (10
µM
) Ho
lder
hum
an m
ilk
Hold
er h
uman
milk
+ c
atal
ase
(1 U
/ml)
Hold
er h
uman
milk
+ H
2O2 (
10 µ
M)
-
‐
-
‐
-
90
76
80
25.10
5 40
10