proteins!. proteins proteins account for more than 50% of the dry mass of most cells monomer: amino...
TRANSCRIPT
Proteins!
ProteinsProteins account for more than 50% of the
dry mass of most cellsMonomer: amino acids
20 amino acids used in cellsCentral carbon atomOne hydrogen attachedAmine (NH2) and carboxylic acid (COOH)
groups attached to carbonOnly thing different is side chain…R-
group (side chain)Responsible for 3D shape of protein
Polymer: polypeptide chains (proteins)Link between monomers is called:
polypeptide bond or PEPTIDE bondMade by a dehydration reaction (between amine group of one aa and
carboxyl group of another aa)
Functions of ProteinsStructural support/strengtheningCatalysts (enzymes)StorageTransportCellular communicationsMovementDefense against foreign substances
Some famous proteins…
Essential components of cell membranes
Oxygen-carrying pigment hemoglobin (haemoglobin)
Antibodies which attack and destroy invading microorganisms
Enzymes!Collagen! (fibrous)
Amino Acids20 -23 major AA (others have been
synthesized in labs)STRUCTURE
Amino group on one end (-NH2)Carboxylic acid group on one end (COOH)HydrogenR-group/side chain (changes)
Glycine
Alanine
Peptide Bond When two amino acids (AA#1 and AA #2) join,
…AA #1 loses a hydroxyl group (-OH) from its
carboxylic acid (-COOH) sideAA #2 loses a hydrogen from its amine (NH2)
groupThe Carbon atom in the carboxyl group of AA#1
is now free to make ONE bond with the Nitrogen of the amine group in AA#2
This bond is called a PEPTIDE Bond H- and –OH removed…what is this???
WATER!!! Another condensation reaction
(dehydration) Dipeptide Molecule New molecule formed by joining 2 amino acids Polypeptide Molecule Molecule made up of many amino acids joined
by peptide bonds Polypeptide is the POLYMER (not protein) Complete protein may contain one polypeptide
chain, twisted and folded or two or more polypeptide chains interacting with each other
Polypeptide bonds
DEHYDRATION!!!
Where are amino acids linked?In living cell
Ribosomes!These reactions that
occur on the ribosomes are controlled by ENZYMES (more on this later)
How are polypeptides broken?
Hydrolysis ReactionsNaturally occurs in
stomach and small intestine
Protein in food is hydrolyzed into amino acids prior to being absorbed by the blood
Once in the blood, these AA can be restructured into polypeptides and then twisted and folded into functioning PROTEINS the cells in you body needs
Protein Structure Primary structure 1’ Order of amino acids in a polypeptide chain
Secondary structure 2’ Polypeptide chain folds because of interactions
between amino acids HYDROGEN BONDING
Tertiary Structure 3’ Gives proteins 3-D shape
VERY IMPORTANT to function of protein Beta pleated sheets and alpha helices fold based on
interactions between R-groups of a.a. Hydrogen bonds, polar/non-polar interactions,
acid/base interactions, disulfide bonds, van der Waals forces
Quaternary Structure 4’ the association of the polypeptide chains some proteins contain more than one polypeptide
chain Each polypeptide chain in the protein is called a
subunit Two or more subunits come together for a specific
function HEMOGLOBIN
On Red blood cells Its shape allows RBCs to carry oxygen all around your
body!
Practice Set 4(6 MC and 1 SAQ)
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