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ReGenesys20137th Technical Meeting, Newcastle, 24 September 2013Pros and cons of different expansion systems for manufacturing of an allogeneic cell therapyJef Pinxteren, Manager and Head R&D2MultiStem cell therapy product: a multimodal biologic productMultiStemProductProfile CelltherapyproductbasedonMultipotentAdultProgenitorCell(MAPC)technology Developingforofftheshelfadministration notissuematchingneeded Expandedproduct withhighproductionyield(e.g.,millionsofdosesfromeachdonor) Longstoragelife canbekeptinfrozenformforyears Consistentsafetyprofile Promoteshealingandtissuerepair throughmultiplemechanismsofaction Notapermanenttransplant cellsclearedfromthebodyovertime Expanded,bankedproduct Obtainedfromhealthy,consentingadult donorsMultiStemCellTherapy Frozenstorage Administeredsystemically(IV)orlocally(catheter,injection,matrix/implant)2013MultiStemImmunologicalIBD/UlcerativeColitisHematopoieticStemCellTransplant/GVHDSolidOrganTransplantDiabetesCardiovascularAMICongestiveHeartFailurePeripheralArteryDiseaseNeurologicalStrokeTraumaticBrainInjuryMultipleSclerosisSpinalCordInjuryOtherBoneAllograft5HT2cAgonists(obesity,other)IMMUNOLOGICALCARDIOVASCULARNEUROLOGICAL20134data2013DataFDAapprovedP2FDAdiscussionsre:P2/3designGermanP1approval3Overview of the MultiStem Production Process4Overview of the MultiStem Production ProcessLotRelease&ProductCharacterizationTestingSterility,Potency,PurityandViabilityStableCytogeneticsAbsenceoftumorgenicpotentialinvivo5Cell Banking ApproachIsolatedMultiStem1donorIsolateMultiStem20PD13daysMasterCellBank20million/vial(200vials)4.109 cells1vial 8PD6daysWorkingCellBank20million/vial(200vials)1vial800.109 cells12PD9daysClinicalDoses>40doses/vial180.106 cells/bag288.1012 cells~1.6.106 doses Requires working in a clean room Labor intensive and numerous open events Manual seeding, feeding and harvesting Limited process control Difficulties in achieving high reproducibility Includes xeno media components (e.g., serum)6Current cell culture process7Images from current MultiStem production process High capacity mfg which will allow for manufacturing runs in the 500 billion 2 trillion cell range (decrease testing costs due to lot size) Removal of xeno/human source materials when possible (lowering of regulatory/safety risk) Determine optimal storage conditions for final product and generate stability data (no special requirements for storage at clinical site) Final container configuration that supports thaw deliver use (bags, vials or other) Automate mfg process (reduce labor costs) Economy of scale for reagents (reduction in materials costs)8Future developmentCourtesyofJonRowley,LonzaHeavy Lifting Scale UpCogs in Scale UpCourtesyofJonRowley,Lonza Stem Cell Research and Assay Development MultiStem identity / cell equivalency (distinguish between stem cells) Molecular mechanisms of MultiStem function Immunology Pre-clinical animal studies Evaluate novel expansion methods Quantum Cell System (Terumo) Microcarrier/Stirred bioreactor Development of xenobiotic free workflow 11The next stage 40LayerCellFactory,Hyperstack directpathtoscaleupwithexistingtechnologies Advantageso Scaledplatformfromcurrentindustrywideexperiencebase Disadvantageso 2dimensionalplatformswithlargevolumemanagementrequirementso Currentlyuntestedlargevolumereductionoptions TFF multiplemanufacturers(Pall,Millipore) kSep promisingbutuntested HollowFiberBioreactor;ATMI(closedsystemstackedpizzaplatform) Advantageso Providesscaleoutcapacitywithsignificantadvantages Eliminatesrequirementforlargescalevolumereduction Closedsystemwithincreasedsafetyforsterilebreach Significantlyreducedlaborcostso Allowsinlinetestingformetabolites,controlofgas/glucoseinrealtime Disadvantageso Limitedscalabilitywithincurrentconfigurationo Bettersuitedforautologousorpatientdesignatedapproach Suspensionbioreactorusingmicrocarriers Advantageso 3Dplatformwithminimalmediaexposure,likelymosteconomicalo Multiplemicrocarrierswithcontrolledsurfacecoatingcomingintomarketo Corning,BD,GEenteringspace Disadvantageso Macroaggregatesleadtocomplicationsinharvest;maybeovercomewithcarriersize(Pluristem)o Difficultyincontrollingoxygenation,shearparametersagainstparticlesizeScale Up Manufacturing Options Phase III Registration trials must be run using commercial production process COGS a very important element of long term competitiveness 12 18 month period required to lock down process Pre-clinical equivalency and biosafety Process qualification and stability studies Clinical equivalency New manufacturing platforms developing with 3 to 6 month generation time!13Sobering thoughts on ManufacturingCell Factories, HyperstackLarge Scale Volume Reduction16Microcarriers 12 microcarriers were tested for cell attachment in dishes The best 3 were tested in small 125 ml spinner flasks The best one was used for expansion in 3 liter bioreactor 12,000 cm culture surface 2.4 liter of medium usedPrimaryScreen:12 SecondaryScreen:3 Proofofconcept:1Quantum System An automated integrated cell culture system Bioreactor, incubator, media and waste management Controlled cell expansion All steps can be programmed and monitored Decrease in labor Closed system Reduction in contamination potential CE marked and FDA listed device17Quantum18Hollow fiber technology Self-contained bench top unit using a hollow fiber bioreactor Small device footprint (48.3 cm x 58.4 cm x 50 cm) Contains 10,000 fibers/bioreactor Total surface is 2.1 m (3255 in) Equivalent of 280 T75 flasks or one 40-stack Smaller surface to volume ratio requires media perfusion Metabolites and gas easily diffuse across fiber19Quantum cell expansion setBioreactorTubing organizerWaste bagHarvest bagGas transfer module20Graphical user interface Graphical user interface makes operation very straightforward Reduced risk of errors Reduced labor Release and harvest of adherent cells takes 20 minutes Potential to run more cell expansion processes simultaneously Key steps in the process are automated e.g. Cell loading, feeding,... Possibility to adjust procedure and save as a custom task21Automation MultiStem cells were seeded in parallel on the Quantum and in standard culture conditions on cell culture plastic22Cell equivalency: Quantum vs plastic Growth on the Quantum is monitored and feed rates may beadjusted based on glucose and lactate levelsHarvest populations were tested on a set of QC assays23Cell equivalency: Quantum vs plasticFlow cytometryCytogenetic analysis on CGH arraysDifferentiationInhibition of T cell proliferationMarker Criterium Standardculture QuantumCD13 >90% 97.51% 99.45%CD34 90% 99.91% 99.92%CD105 >90% 99.53% 95.76%HLAI 24Reproducibility#cellsseeded(x106) #cellshavested(x106) Run time(h)8.45 752 1467.28 782 1488.57 753 1443 independent runs from the same donor generate the same harvest yields25MultiStem isolation from bone marrowWith prior density centrifugation Whole bone marrow loadWholeBMloadonQuantumasgoodasseedingBMMNConcellcultureplastic26Cell equivalency of MultiStem isolation: Quantum vs plasticAngiogenesis assayDifferentiationFlow cytometryInhibition of T cell proliferationMarker Criterion Plastic QuantumCD13 >90% 99.65% 98.93%CD34 90% 98.19% 99.17%CD105 >90% 98.82% 99.11%HLAI Cells from 3 donors tested Donor 1 3 consecutive runs on the Quantum starting from bone marrow in run 1 Cells cultured on regular plastic Donor 2 2 runs starting from the same seeding stock (run 1 & 2) Run with different type of coating which was not successful (run 3) Cells cultured on regular plastic Donor 3 Isolation on the Quantum Isolation on plastic MSC isolated on plastic HUVEC as control27Microarray analysis28Microarray analysis - DendrogramHUVECDonor 3 MSCDonor 2 run 3Donor 3 plasticDonor 2 plasticDonor 1 plasticDonor 3 run 1Donor 1 run 1Donor 1 run 3Donor 1 run 2Donor 2 run 1Donor 2 run 2 Hollow fibers large surface area maximizes cell expansion in a minimal amount of space MultiStem cells can be isolated and expanded on the Quantum MultiStem cells show the same growth kinetics and retain all cell characteristics Automation: ease of use and better control Decrease in labor: 2 hours of lab work to generate an average of 800 million cells Expensive clean rooms not required29Conclusions MultiStem and MSC derived from same donor bone marrow Differences are of epigenetic origin Further describing MultiStem identity Transcriptome miRnome Methylome Proteome30Omics screenings : MultiStem vs MSC3.8x1093.3x101430 PCA to monitor cell equivalency of different cell culture methods31Transcriptome : comparability after culture manipulationPlasticexpandedQuantumexpandedMSCHUVECFirstPrincipalComponentSecondPrincipalComponent32MultiStem versus MSC : Gene Set Enrichment analysisMultiStemMSCMSC more mature?Telomerase activitymaintenanceProliferation Telomerase activity is preserved in XF-MS33Telomerase activityy i o y i o y i o01.01072.01073.0107MSC MultiStem XenofreeMultiStemTelomerase activity (copy n) Identify markers by comparing differential expression between Multistemand MSC Extract key miRNAs Consistent effect in 3 donors Develop multiplex qPCR as xeno-free equivalency assays Gene Set Enrichment Analysis Identify mRNA targets of miRNA Gene Set enrichment / Pathway analysis34Application of miRNA expression datamiRNA screening: Positive MultiStem markers Negative MultiStem markersUsing two different platforms, array and PCR-based, we are currently exploring the possibility of using miRNA profiles to confirm the uniqueness of our product. 35omics screening36miRNA marker screening strategyqPCR ArrayTotaltargets 755 1400Detectedtargets 207 847Differentialexpression 160 140Donorindependent,FC>1.5 67 3218 510MS>MSC33 116MSC>MSqPCR array qPCR array2: MultiStem marker identificationPositive markers Negative markers1: miRNome + data analysis4 : Use panel of top markers in cell comparability testing 3 : Validate 16 miRs by qPCR in independent donors top markers miRNA marker expression is maintained during : Regular expansion Xeno-free expansion Bioreactor expansion37miRNA expression qPCRRelative expression0.00.10.20.30.40.5Y O Y OMS XFCES MSCdonor A donor BY O Y OMS XFCES MSC Y O Y OMS XFCES MSCRelative expression0.00.20.40.60.81.0Y O Y OMS XFCES MSCY O Y OMS XFCES MSC Y O Y OMS XFCES MSCdonor A donor BGenome-wide DNA methylation analysisFind epigenetic markers for distinguishing MultiStem from competing productsand proof epigenetic stability during expansions38omics screening39Methylome : Multi-layered epigenetic regulation of MultiStem expansion capacity40Application of omics data in product development41AcknowledgmentsReGenesys: BartVaes,DavidCraeye,AnneliesBogaert,AlineVisser,SaartjeWalbers,PeterSterkendries,MarianCrabb,LiesbethVandenpoel,KristelGijbels,EllenVanHoutven,LienTimmermanAthersys: BobDeans,TonyTing,JonRowley,WillieMayswww.regenesys.eu

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