Z 7

00 0

6 2

1 (4

)

Pre-incubation: 1 cycleStep 1: 95 °C for 10 min

Amplifi cation: 40 cyclesStep 1 : 95 °C for 15 secStep 2*: 60 °C for 60 sec

* Fluorescence detection1 cycle

10 min 15 sec | 60 sec*

40 cycles

95 °C

60 °C

37 °C

Bacillus cereus Detection Kit Quick Reference Procedure

PCR kit for the qualitative detection of groEL target using real-time PCR instruments.

Before starting, it is strongly recommended to read the entire product manual available on our website.

PROGRAM SETUPProgram your real-time PCR instrument before setting up the PCR reactions. Select the following channels:

u FAM (groEL) and VIC/HEX (Internal Control).

Order no. Z 700 06

- 5‘Nuclease -

Version 4, November 2017

DATA INTERPRETATIONVerify results of positive (Control Template) and negative controls (H2O), before interpreting sample results. Always compare samples to positive and negative control. Review data from each channel and interpret results as described in the table.

foodproof® SL

FAM VIC/HEX Result Interpretation

+ + or - Positive for groEL

- + Negative for groEL

- - Invalid

foodproof® is a registered trademark of BIOTECON Diagnostics. The above mentioned real-time PCR instruments are registered trademarks of their respective holders.

foodproof® SLBacillus cereus Detection Kit

Z 700 06Kit for 50 reactionsStore kit at -15 to -25 °C

For food testing purposesFOR IN VITRO USE ONLY

BIOTECON Diagnostics GmbH | Hermannswerder 17, 14473 Potsdam, Germany | +49 331 2300 200 | www.bc-diagnostics.com

3. ADD SAMPLES AND CONTROLSPipet 6 µl of samples, negative control (H2O PCR-grade; not included) or

Control Template (C) into respective wells.

2. ADD PCR MIXPipet 14 µl of prepared PCR mix into each strip or plate well.

PREPARATION OF THE RT-PCR MIXTake appropriate precautions to prevent contamination, e.g. by using fi lter tips and wearing gloves. Thaw reagents, mix (do not vortex!), and briefl y spin vials before opening.

4. SEALSeal strips/plate accurately.

5. CENTRIFUGEBriefl y spin strips/plate in a suitable centrifuge.

6. START REAL-TIME PCR RUNCycle samples as described above.

+14 µl

+6 µl

+10 µl + 4 µl

1. PREPARE PCR MIX

(n samples + 2 controls + at least one additional reaction to cover pipetting loss). Mix carefully but thoroughly by pipetting up and down.

for each reaction to a suitable tube.

Add 10 µl Master Mix (2XM), 4 µl Primer/Probe Mix (P) and