project stink-be-gone june training - shrewsbury lab...•named “stink” bugs because of the odor...
TRANSCRIPT
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Project Stink-be-GoneJune training
Dr. Rebeccah Waterworth
Email address: [email protected]
Project website address:
http://shrewsburylab.weebly.com/
project-stink-be-gone.html
Street Address:
University of Maryland
4112 Plant Sciences Bldg
4291 Fieldhouse Dr.
College Park, MD 20742
Agenda1. Pre-quiz
2. Training with Powerpoint (Part 1)• Stink bug species
• Stink bug life cycle
• Egg mass “look-alikes”
• ID different stink bug egg masses
3. Walkabout outside to look for egg masses
4. Training with Powerpoint (Part 2)• Natural enemies of stink bug eggs
• Where to look for egg masses and on what hosts
• Field work lists
• Collecting periods
• The datasheet (site types, habitat features, wasp guarding)
• GPS coordinates
• Shipping via FedEx
5. Post-quiz
6. Evaluation of training
7. Pick-up kits for project
mailto:[email protected]://shrewsburylab.weebly.com/project-stink-be-gone.html
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What are stink bugs?• Order Hemiptera
• Family Pentatomidae
• Herbivorous and predatory species
• Have a shield-shape to their body
• Named “stink” bugs because of the
odor released from glands on the
underside of their bodies
• Sometimes brightly colored
Anna Letaw Bill Johnson
Check out the stink bug look-alikes in your spiral notebook and at the training
Stink bug adultsp. 1p. 3p. 7
p. 12p. 19 p. 21
Page numbers refer to the
page in your stink bug book
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Stink Bug Life Cycle
Managing stink bugsBiological control (or biocontrol)
• Natural enemies include predators and parasitoids
• In Project Stink-be-Gone, we are interested in parasitoids
that attack the eggs of stink bugs
David R. Lance USDA APHIS PPQ David R. Lance USDA APHIS PPQElijah Talamas
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Collecting Egg Masses – Stink bugsBrown marmorated
stink bugs
(BMSB)
Harlequin stink
bugs (HQN)
Brown stink bugs
Spined soldier bug
(Podisus)
Look for the barrel shape when the eggs are viewed in profile
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Collecting Egg Masses –Stink bug egg “look-alikes”
But if you are in doubt as to whether an egg mass was laid
by a bug, beetle, or moth, please send it to me anyway!
Other bug eggsSee display at the end of the
training to see what these
bugs and their eggs look like!
• Various assassin bugs
• Including green assassin
bugs
• Leaf-footed bugs
• Squash bugs (no need to
collect and send these egg
masses)
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Other “stuff”
Braconid
wasp pupal
cases
Green
lacewing
pupal
cases
eulophid
wasp pupal
casesErineum galls
(sample is oak)
Some stink bug natural enemiesPredators Parasitoids
Chewing predator
= ground beetles
Piercing/sucking
Piercing/sucking
Other stink bugs
Spiders
See parasitoid
specimens
under the
microscope!
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Egg Masses Attacked by Natural Enemies
a: bugs hatched; b: feeding by a piercing sucking predators; c: chewing predation,
e: parasitiods emerged (note circular emergence holes and no egg burster)
Egg burster
Collecting Egg Masses – Where?
Edges are better places to search
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Collecting Egg Masses – What hosts?
Any mustards, including
sweet alyssum! Cleome, too
Pretty much everything!
Host Plants of BMSB
Trees/shrubs PerennialsAnnual
Flowering Plants
Annual,
VegetablesVines, Various
Cercis
canadensis (8)
Red raspberry
(2)Cleome (5) Tomato (1) Vitis riparia (2)
Acer rubrum (4)Wine
raspberry (2)Lantana (1)
Virginia
creeper (1)
Magnolia (1)Asclepias
syriaca (1)
Pigweed*
(Amaranthus) (1)
Tilia cordata (1)
Paulownia
tomentosa (2)
Cornus
racemosa (1)
Pyrus
calleryana (1)
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Collecting Egg Masses – What hosts?BMSB: polyphagous pest, but not known
to feed on conifers or ornamental grasses(partial list) Along wooded edges, check out tree
of heaven, sassafras, black cherry, black locust,
hackberry, catalpa, paulownia, Eastern redbud
Brown stink bug: polyphagous pest(partial list) sassafras, wineberry, Eastern
redbud, yellowwood, gray dogwood, red maple,
hackberry, sumac, butternut, boxelder, black
cherry, linden, paulownia, catalpa, raspberry,
black walnut, elm, peach
Green stink bug: polyphagous pest, BUT
not known to occur on conifers or oaks(partial list) Eastern redbud, black cherry,
red maple, sassafras, raspberry, persimmon,
Hydrangea paniculata, Washington hawthorn,
flowering dogwood, American witchhazel,
Carolina allspice, dogwood, apple, black walnut
Collecting Egg Masses – What hosts?
Rough stink bug: polyphagous pest(partial list) Eastern redbud, birch, red maple,
American sweetgum, tree of heaven, littleleaf
linden, paulownia, magnolia, dogwood,
Kentucky coffeetree,
Spined soldier bug: predator of other
insects but eggs laid on plants.(Partial list) Norway maple, littleleaf linden,
apple
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Field Work
What we supply What you should bring
Yellow cooler Clipboard
One ice pack Several datasheets
Petri dishes Pencils
Box to ship cooler Scissors
Pre-paid FedEx label Hand lens/loupe
Smart phone (for GPS coordinates)
Hand-held pruning shears or loppers
For safety and comfort:
Hat, long sleeves and pants
Sunscreen
Water
Insect/tick spray
Collecting Periods and Times• Search and collect egg masses any
time during the day
• Must be sure you can ship later that
day (or if you collect on Sundays –
first thing on Monday)
• Find one or two egg masses at a
time?
o Hold egg masses for up to one
week in the fridge
o Try to collect more eggs the
following week.
o Keep egg masses in petri dishes
inside cooler bag.
*Optional sampling period.*
Please consider searching
1 – 2 hours during this
period.
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Collecting Periods and Times
Prioritized sampling period = 6 weeks.
Please search one hour each week for
egg masses.
What are woods?
Yes!
Yes!
No!
No!
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The Datasheet• Each datasheet holds four tables or sections, one for
each of four different sites that you decide to visit.
• If you think that you might visit more than four
different sites when you sample for one hour, take
more copies of the datasheet with you.
Front Back
Site 1
Site 2
Site 3
Site 4
The Datasheet
When you have finished collecting egg masses and
filled in the datasheet, scan, copy, or take a picture of
your datasheet for your records.
Please send the original copy of the datasheet to me
IN YOUR COOLER.
If you visit a site and don’t see any egg masses, we
still would really like to know what site you visited:
approximate square area searched, a site description,
and features in that landscape. Please still fill out the
top two rows of that site section on your datasheet.
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Datasheet: Site TypesPrivate yardMultiple plant types – search equally
among herbaceous plants, shrubs, and
trees. If wooded edges are present,
search along those, too.
Park with woodsMultiple plant types – search equally
among any of the plant types present
Datasheet: Site TypesPark without woodsFairly simple landscape, but likely
multiple plant types are present –
search equally among any of the plant
types present
Wooded edge only• No ornamental plantings or
agriculture outside of the woods.
• Only search trees and shrubs that are
at the edge.
• Known hosts of BMSB are tree of
heaven, sassafras, black cherry,
hackberry, black locust, catalpa, and
paulownia
• No need to actually enter the woods.
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Datasheet: Site Types
Farm/Agriculture setting: • Corn and soybeans are some of the
dominant crops in Maryland.
• Search only along the edges of the fields, especially when corn
has developing ears and soybeans
have flowers and pods.
• Many farm fields have wooded
borders.
• If you can safely access those, please
search along those edges, too.
Feel free to collect in other
counties other than the one where
you are a Master Gardener. Just
write down the collection
information on your datasheet.
Search area measurements
160 feet
45 feet
130 feet
160+45+130 = 335 ft
335 ft x 2 ft = 670 ft2
75 feet
75 ft x 2 ft = 150 ft2
150 ft2 + 670 ft2
= 820 ft2
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How did I get those measurements?
Small ruler icon =
show ruler
Google Earth,
not Google
Maps
https://www.go
ogle.com/earth/
desktop/
How did I get those measurements?
Change ruler to
measure the
distance in feet.
https://www.google.com/earth/desktop/
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How did I get those measurements?
Read
measurement!
Habitat Features√ Paved surface
Manmade structure
? Flower trees
? Fruiting trees
√ Unmanaged trees
√ Managed trees
? Flower herb plants
? Flowering shrub
? Fruiting shrub
? Crop/vegetable
√ Managed turf
Open meadow
Stream/creek
Check all habitat features that were in
or bordering (within 5 feet) the area that
you searched
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Datasheet: Wasp Guarding?
• Try to catch the small
wasp (=parasitoid) if you
can and keep it with the
egg mass.
• If you can’t catch it,
that’s okay.
• But, please check the
box next to the egg mass
that you are collecting to
say that one was there.
Egg Mass is too Large for the Petri Dish!
Perfect fit!Some trimming
required!
Cutting is
preferred over
ripping the leaf
because the egg
mass is less
likely to pop off.
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Datasheet: GPS coordinates
Free Apps (for both iPhones and Android devices)
Google Maps
Waze
Here WeGo (Maps)
InRoute
Scout GPS
MapQuest
Please report by degrees North and degrees
West on your datasheet for each site.
Google Maps reports values in decimal form,
like this: 38.9889 (N) -76.9409 (W)
GPS Coordinates: Google Maps
Open Google Maps
so that it finds your
location
Place your finger and
hold on your location
(an address or ‘dropped
pin’ shows up)
Swipe up at the
bottom
North West
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Packing your Cooler• Shipping label is a giant sticker that is placed on the SIDE of your box.
• Your box will be sent to you with a label that is already filled out to send
samples to me.
• Remove and keep the top copy of the label (it says “Sender’s Copy” in
the top right corner.
• Feel free to use any
material to pad the
petri dishes and the
ice pack in your box.
• Balled up paper, paper
towels, and packing
peanuts are fine.
• If you hold the box
closed and shake it
(and you don’t hear
any rattling), the petri
dishes should ship
okay.
FedEx ShippingFedEx Express (orange) NOT FedEx Ground (green)
Zip code goes here
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FedEx Shipping
Green equals FedEx Ground.
Do not use any FedEx
Ground shipping times. Only use FedEx Express
(orange) shipping times.
FedEx Shipping
Mon Tues Wed Thurs Fri Sat Sun
Collect
and ship × × ×
Not
preferred
(for
shipping)
Not
preferred
(for
shipping)
Not
preferred
(for
shipping)
×(ship on
Mon.)
Arrives Wed Thurs Fri
Sat (but
no delivery
at Univ.)
Mon
(3 days
in transit-
too long)
Tues
(3 days in
transit –
too long)
Wed
FedEx Express 2day = 2 business days in transit