progress since the february 2005 london dna barcode of life conference scott miller, chair...
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Progress since the Progress since the February 2005 February 2005
London DNA Barcode London DNA Barcode of of
Life ConferenceLife ConferenceScott Miller, ChairScott Miller, Chair
Consortium for the Barcode Consortium for the Barcode of Lifeof Life
Smithsonian InstitutionSmithsonian Institution
CBOL’s HistoryCBOL’s History• 2003: First barcoding publications2003: First barcoding publications• 2003: Sloan Fdn supports Banbury workshops2003: Sloan Fdn supports Banbury workshops• 2004: Sloan 2-year inaugural CBOL grant2004: Sloan 2-year inaugural CBOL grant• 2004: Secretariat opens at Smithsonian2004: Secretariat opens at Smithsonian• 2005: International conference London2005: International conference London• 2006: Sloan 2-year renewal grant2006: Sloan 2-year renewal grant
• Now an international affiliation of:Now an international affiliation of: Natural history museums, biodiversity Natural history museums, biodiversity
organizationsorganizations Users: e.g., government agenciesUsers: e.g., government agencies Private sector biotech companies, database Private sector biotech companies, database
providersproviders
First International Barcode of First International Barcode of Life Conference, NHM LondonLife Conference, NHM London• 240 participants from 44 countries240 participants from 44 countries• 38 CBOL Member Organizations38 CBOL Member Organizations• Announced plans for All-Fish and All-Announced plans for All-Fish and All-
Birds initiativesBirds initiatives• 30,000 barcode records in BOLD 30,000 barcode records in BOLD
from ~10,000 speciesfrom ~10,000 species• First evidence of effectiveness of First evidence of effectiveness of
barcoding for identificationbarcoding for identification
Growth of CBOLGrowth of CBOL• Executive Committee from 4 continentsExecutive Committee from 4 continents• Scientific Advisory Board from 13 countriesScientific Advisory Board from 13 countries• 159 Member Organizations from 50 159 Member Organizations from 50
countriescountries• Outreach meetings to Africa, South/Central Outreach meetings to Africa, South/Central
America, AsiaAmerica, Asia• Now 54 Member Organizations from 23 Now 54 Member Organizations from 23
LDCsLDCs• Network of 16 “Leading Labs”Network of 16 “Leading Labs”• Strong partnerships with Strong partnerships with
GenBank/EMBL/DDBJ, GBIF, EOLGenBank/EMBL/DDBJ, GBIF, EOL
CBOL’s Mission:CBOL’s Mission:Promoting DNA BarcodingPromoting DNA Barcoding
as a Global Standard as a Global Standard1.1. Developing/raising community standardsDeveloping/raising community standards2.2. Barcode projects to populate databaseBarcode projects to populate database3.3. Global participation and coordinationGlobal participation and coordination4.4. Acceptance by taxonomic communityAcceptance by taxonomic community5.5. Coordination with other fields of scienceCoordination with other fields of science6.6. Adoption by regulatory agenciesAdoption by regulatory agencies7.7. Product development by private companiesProduct development by private companies
CBOL Member Organizations: 2007
• 150+ Member organizations, 50 countries
• 50+ Member organizations from 20+ developing countries
CBOL StructureCBOL Structure
Member Organizations
Executive Committee
Working Groups
Scientific Advisory Board
Secretariat Office
Growth of BOLD, Barcode Growth of BOLD, Barcode DataData
• Now ~300,000 BOLD records from Now ~300,000 BOLD records from 31,000 species31,000 species
• FISH-BOL has barcoded 14% of speciesFISH-BOL has barcoded 14% of species• ABBI has completed North American ABBI has completed North American
birdsbirds• Tephritids and Mosquitos underwayTephritids and Mosquitos underway• BARCODE data standards adoptedBARCODE data standards adopted• Now thousands of fully compliant Now thousands of fully compliant
recordsrecords
Biodiversity Informatics:Biodiversity Informatics:Fragmented, UnconnectedFragmented, Unconnected
Journal Publication
Species Name
Voucher Specimen
??
Type specimens
Varied species concepts: - BSC (hard to apply) - Typology - Genetic lineages
DNA Barcodes:DNA Barcodes:A Key Variable for Biodiversity A Key Variable for Biodiversity
InformaticsInformatics
Journal Publication
Species Name
Voucher Specimen
Barcode Sequence
Authority files of taxonomic
names
Museum databases of
associated dataDatabases of species
occurrences and distribution
Barcode Sequence
Voucher Specimen
Species Name
Specimen Metadata
Literature(link to content or
citation)
BARCODE Records in GenBank
Indices - Catalogue of Life - GBIF/ECAT
Nomenclators - Zoo Record - IPNI - NameBank
Publication links - New species
GeoreferenceHabitat
Character setsImages
BehaviorOther genes
Trace filesOther
DatabasesPhylogenetic
Pop’n GeneticsEcological
Primers
Databases - Provisional sp.
Links to Taxonomic LiteratureLinks to Taxonomic Literature• London meeting on electronic access London meeting on electronic access
to taxonomic literature, 2005to taxonomic literature, 2005• Catalyzed Biodiversity Heritage Catalyzed Biodiversity Heritage
LibraryLibrarywww.biodiversitylibrary.orgwww.biodiversitylibrary.org
• Proactive steps with PubMed to add Proactive steps with PubMed to add taxonomic journals to online abstractstaxonomic journals to online abstracts
• Aggressive negotiation with Aggressive negotiation with publishers of barcoding paperspublishers of barcoding papers
• Involvement in Encyclopedia of LifeInvolvement in Encyclopedia of Life
Adoption by RegulatorsAdoption by RegulatorsInternationalInternational• FISH-BOL and fish regulatory agenciesFISH-BOL and fish regulatory agencies
CBOL workshop in Taipei, September 2007CBOL workshop in Taipei, September 2007• FAO and International Plant Protection FAO and International Plant Protection
ConventionConvention Proposal for Diagnostic Protocols for fruit flies Proposal for Diagnostic Protocols for fruit flies
• CITES, National Agencies, Conservation NGOsCITES, National Agencies, Conservation NGOs International Steering Committee, identifying pilot International Steering Committee, identifying pilot
projectsprojectsNational examplesNational examples• US Federal Aviation Administration – BirdsUS Federal Aviation Administration – Birds• US Environmental Protection Agency – Aquatic US Environmental Protection Agency – Aquatic
insectsinsects• US Department of Agriculture – Fruit fliesUS Department of Agriculture – Fruit flies
Preview of the Third Preview of the Third International Barcode International Barcode
Conference (2009)Conference (2009)• 1 million barcode records1 million barcode records• 100,000 species100,000 species• Identifying unknowns in 30 minutes for less Identifying unknowns in 30 minutes for less
than US$1than US$1• Barcoding is a validated lab procedureBarcoding is a validated lab procedure• Port inspectors starting to test unknowns Port inspectors starting to test unknowns
with barcodes in several countrieswith barcodes in several countries• Portable sequencing ?Portable sequencing ?• Completion of the first local barcode biotic Completion of the first local barcode biotic
inventoryinventory