PROGRESS IN BASIC AND CLINICAL IMMUNOLOGY

ADVANCES IN EXPERIMENTAL MEDICINE AND BIOLOGY

Editorial Board:

NATHAN BACK, State University of New York at Buffalo

IRUN R. COHEN, The Weizmann Institute of Science

DAVID KRITCHEVSKY, Wistar Institute

ABEL LAJTHA, N. S. Kline Institute for Psychiatric Research

RODOLFO PAOLETTI, University of Milan

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Volume 490 MECHANISMS OF LYMPHOCYTE ACTIVATION AND IMMUNE REGULATION VIII

Edited by Sudhir Gupta

Volume 491 THE MOLECULAR IMMUNOLOGY OF COMPLEX CARBOHYDRATES-2

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Volume 494 THE NIDOVIRUSES (CORONA VIRUSES AND ARTERIVIRUSES)

Edited by Ehud Lavi, Susan R. Weiss, and Susan T. Hingley

Volume 495 PROGRESS IN BASIC AND CLINICAL IMMUNOLOGY

Edited by Andrzej Mackiewicz, Madej Kurpisz, and Jan Zeromski

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PROGRESS IN BASIC AND CLINICAL IMMUNOLOGY

Edited by

Andrzej Mackiewicz University School 0/ Medical Sciences GreatPoland Cancer Center Poznan, Poland

Maciej Kurpisz Institute 0/ Human Genetics Polish Academy 0/ Sciences Poznan, Poland

and

J an Zeromski University School 0/ Medical Sciences Department o/Immunology Poznan, Poland

Springer Science+Business Media, LLC

Library of Congress Cataloging-in-Publication Data

Progress in basic and clinical immunology/edited by Andrzej Mackiewicz, Maciej Kurpisz, and Jan Zeromski.

p. ; cm. - (Advances in experimental medicine and biology, ISSN 0065-2598; v. 495) "Proceedings of the 14th European Immunology Meeting (EFIS 2000) held September

23-27,2000, in Poznan, Poland"-T.p. verso. Includes bibliographical references and index. ISBN 978-1-4613-5194-8 ISBN 978-1-4615-0685-0 (eBook) DOI 10.1007/978-1-4615-0685-0

1. Immunology-Congresses. 2. Clinical immunology-Congresses. I. Mackiewicz, Andrzej. 11. Kurpisz, Maciej. III. Zeromski, Jan, 1938- IV. European Immunology Meeting (14th: 2000: Poznan, Poland) V. Series.

[DNLM: I. Immunity, Cellular-Congresses. 2. Autoimmunity-Congresses. 3. Dendritic Cells-physiology-Congresses. 4. Receptors, Immunologic-Congresses. 5. T-Lymphocytes-physiology-Congresses. QW 568 P964 2001] QR180.3 .P76 2001 616.07'9-dc21

2001038777

Proceedings of the 14th European Immunology Meeting (EFIS 20(0), held September 23-27, 2000, in Poznan, Poland

ISSN: 00652598

ISBN 978-1-4613-5194-8

©2001 Springer Science+Business Media New York Originally published by Kluwer Academic I Plenum Publishers, New York in 2001 Softcover reprint ofthe hardcover 1st edition 2001

http://www.wkap.nl

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No part of this book may be reproduced, stored in a retrieval system, or transmitted in any form or by any means, electronic, mechanical, photocopying, microfilming, recording, or otherwise, without written permission from the Publisher

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v

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Preface

Progress in Basic and Clinical Immunology is a result of the 14th European Immunology Meeting - EFIS 2000 held in Poznan, Poland on 23-27 September 2000. EFIS 2000 gathered over 1400 immunologists from all over the world. It was an exceptionally memorable meeting for a number of reasons: 1) it was held in the last year of the century and the millennium, thus provoking conclusions of past achievements of immunology and projections for the future; 2) it was held in Poland, a country that is a symbol of struggle for freedom for a large number of scientists originating from the "Eastern Bloc" countries and 3) EFIS celebrated its 25th anniversary at this occasion.

The scientific program included: Key Note Lecture by Peter Krammer (Germany), 9 State-of-Art Lectures including Rolf M. Zinkernagel (Switzerland) - Nobel Prize Laureate - lecture entitled "Immunity 2000", 14 Meet an Expert Sessions, 80 Plenary Lectures and 47 Workshops. Altogether 875 communications were presented. The philosophy of the scientific program did not follow the usual routine of immunology meetings. Topics of the plenary sessions were selected on the basis of recent developments rather than historical background. On the other hand Workshops were designed on the basis of abstracts submitted. We invited 117 authors to submit their presentations for publication in Progress in Basic and Clinical Immunology -62 were received and are included in this book. Most of the papers are related to the tumour immunology and immunotherapy that reflects a major focus of submitted abstracts for EFIS 2000. Of course, not all the very valuable communications could be included in the volume. However, we trust that the selection that we have offered will be at least in part

Vll

viii Preface

representative of the meeting and will pave the way for further developments.

Andrzej Mackiewicz Maciej Kurpisz

Jan Zeromski

Contents

T-CELLS

CD3-dependent regulation of early TCRn gene expression in mainstream aO and NKaO T cell development........... •••••••• ••• 5

Nicole Baur and Klaus Eichmann

Molecular characterization of gut T cell precursors in euthymic and athymic mice..................................................... ..... ... 15

Florence Lambolez and Benedita Rocha

CRTH2: marker for the detection of human Th2 and Te2 cells..... •••.•••••••••. •••••••••.••.•.•.. •••.•••••.•.•••••• •••••.••. •••.•.•• 2S

Lorenzo Cosmi, Francesco Annunziato, Grazia Galli, Roberto Manetti, Enrico Maggi and Sergio Romagnani

GILZ, a glucocorticoid hormone induced gene, modulates T lymphocytes activation and death through interaction with NF-kB.......... ........•...•........•..........••..................•••••. 31

Carlo Riccardi, Stefano Bruscoli, Emira Ayroldi, Massimiliano Agostini and Graziella Migliorati

Tyrosine phosphorylation of PICOT and its translocation to the nucleus in response of human T cells to oxidative stress.............................................................. .............. 41

Yael Babichev and Noah Isakov

ix

x Contents

IMMUNE RECEPTORS

The molecular specificity of IgG-Fc interactions with Fey receptors ................................................................ 49

Yusuke Mimura, Rodolfo Ghirlando, Peter Sonderrnann, John Lund and Roy Jefferis

Regulation of myeloid cell proliferation and survival by p75/ AIRMl and CD33 surface receptors. .... ..... ...... ...... .... ..... 55

Maria Cristina Mingari, Chiara Vitale, Chiara Romagnani, Michela Falco and Lorenzo Moretta

X-linked lymphoproliferative disease: the dark side of 2b4 function........................................................... ........ 63

Cristina Bottino, Silvia Parolini, Roberto Biassoni, Michela Falco, Luigi Notarangelo and Alessandro Moretta

ANTIGEN PRESENTATION / DENDRITIC CELLS

Role oftapasin in MHC class I antigen presentation in vivo............ 71 Natalio Garbi, Pamela Tan, Frank Momburg, Gunter J. Hiimmerling

Regulation of transporters associated with antigen processing (TAPs) by nucleotide binding to, and hydrolysis by, Walker consensus sequences......................................................... 79

Loredana Saveanu and Peter M. Van Endert

The AIR-J encoded class II transactivator (CIITA): the master coordinator of MHC class II gene expression and .. more..... .... ... 83

Roberto S. Accolla

Major histocompatibility complex class II (MHC II) expression during development of human fetal brain and haemopoietic organs..................................................... ................... ... 93

Teresa Wierzba-Bobrowicz, Elzbieta Kosno-Kruszewska, Eliza Lewandowska, Waldemar Lechowicz, Bogna Schmidt-Sidor

Interactions between dendritic cells...................................... .... 103 Stella C. Knight, Fiona Burke and Penelope A. Bedford

Contents Xl

Neural regulation of dendritic cell function.................... •.••........ 111 Georges 1M Maestroni

A central role for heat shock proteins in host deficiency .•••.••....••••• 121 Pramod K. Srivastava

CYTOKINES

Allele specific regulation of cytokine genes: monoallelic expression of the II..-IA gene......... ..•........•.••.•...•.....••....... ... 129

Comelis L. Verweij, Jean-Pierre Bayley, Aleida Bakker and Eric L. Kaijzel

Effect of polysaccharide sulfates on the production of interleukin-8 in an ex vivo model...................................... ••• 141

Pawel P. Jagodzinski, Wieslaw H. Trzeciak

Coordination of interleukin-6 biology by membrane bound and soluble receptors..... ...... ••.• •••.• •••.•••.. •..•..... ......•..••• •...•. 145

Stefan Rose-John

Termination and modulation of II..-6-type cytokine signaling.... ••• ..• 153 Peter C. Heinrich, Johannes Bode, Manuela Decker, Lutz Graeve, Astrid

Martens, Gerhard Miiller-Newen, Stefan Pflanz, Fred Schaper, Jochen Schmitz

Cytokine-induced STAT signalling through the cytoplasmic compartment.............................................................. .... 161

Pravin B. Sehgal

Cytokine-mediated growth inhibition of human melanoma cells...... 169 Marcin Kortylewski, Peter C. Heinrich, Andrzej Mackiewicz and Iris

Behrmann

Interleukin-6, tumor necrosis factor a and interferon.., in patients with anorexia nervosa........... ...•...........•..• •••••••....... 173

Renata Komorowska-Pietrzykowska, Andrzej Rajewski, Magdalena Sobieska and KrzysztofWiktorowicz

Interleukin-l system in testis - quantitative analysis..................... 177 Natalia Rozwadowska, Dorota Fiszer, Maciej Kurpisz

xu Contents

IMMUNODEFICIENCES

Mapping genes underlying complex disorders: progress on IgA deficiency and common variable immunodeficiency .................. 183

I. Vorechovsky, A. D. B. Webster, L. Hammarstrom

Ataxia-Telangiectasia ............................................ " ............. 191 Sara G. Becker-Catania and Richard A. Gatti

Gene therapy of severe combined immunodeficiencies.................. 199 Alain Fischer, Salima Hacein-Bey, Fran<;:oise Le Deist, Genevieve

De Saint Basile and Marina Cavazzana-Calvo

Immune reconstitution after highly active anti-retroviral treatment of HIV infection ................................................. 205

Brigitte Autran, Guislaine Carcelain and Patrice Debre

Dissimilarity of cinical signs with parameters characterizing immune system............................................................... 213

Natalia K. Gorlina, Anatoly N. Cheredeev, Ivan G. Kozlov, Alexander R. Guskov

AUTOIMMUNITY

The role of Fc receptors and complement in autoimmunity............ 219 Ulrich Baumann and Reinhold E. Schmidt

Effect of Hsp70-peptide complexes generated in vivo on modulation EAE .............................................................. 227

Grazyna Galazka, Agata Walczak, Tomasz Berkowicz and Krzysztof Selmaj

Gene transfer of protective cytokines in rheumatoid arthritis ......... 231 Oliver Distler, Christian A. Seemayer, Thomas Pap, Renate E. Gay,

Michel Neidhart, UlfMiiller-Ladner and Steffen Gay

Modification of humoral antisperm response.............................. 241 Beata Grygielska, Dorota Fiszer, Alina Domagala, Maciej Kurpisz

Contents xiii

ALLERGY / INFLAMMATION

Immunologic mechanisms of allergen-specific immunotherapy.. ...... 247 C. A. Akdis and K. Blaser

Phosphorothioate oligonucleotides: looking for the motif(s) possessing immunostimulatory activities in humans.................. 261

Francesca Brugnolo, Francesco Annunziato, Salvatore Sampognaro, Cinzia ManuelIi, Lorenzo Cosmi, Sergio Romagnani, Enrico Maggi and Paola Parronchi

Disorders in mononuclear phagocytes and reduced glutathione and their correction in Chernobyl children with recurrent respiratory infections and chronic inflammatory focal lesions.. ... 265

Viktor Chemyshov, Lyudmila Omelchenko, Gemot Treusch, Maxim Vodyanik, Tatyana Pochinok, Marina Gumenyuk, Gennady Zelinsky

IMMUNOTHERAPY

Adoptive transfer of mucosal T cells or dendritic cells from animals fed with cholera toxin B subunit alloantigen conjugate induces allogeneic T cell tolerance.................... ....... 271

Bin-Ling Li, Jia-Bin Sun and Jan Holmgren

Total body irradiation before bone marrow transplantation: aims and results................................... ............................ 277

Julian Malicki, Jacek Wachowiak, GraZyna Kosicka, Grazyna Stryczynska, Agnieszka Nowak and Jacek Pracz

Statins as immunomodulatory drugs........................................ 283 Andrzej Gorski, Monika Kasprzycka, Maria Nowaczyk, Piotr

Wierzbicki, GraZyna Korczak-Kowalska, Pawel Jezak, Danuta Klosowska, Aneta Ekiert, Konrad Klodos

VACCINES

Phage display of epitopes from HIV -1 elicits strong cytolytic responses in vitro and in vivo ........................................ ..... 291

John Guardiola, Piergiuseppe De Berardinis, Rossella Sartorius, Cristina Fanutti, Perham Richard N. and Giovanna Del Pozzo

XIV Contents

Oral immunization of human with transgenic lettuce expressing hepatitis B surface antigen................................................. 299

JozefKapusta, Anna Modelska, Tomasz Pniewski, Magdalena Figlerowicz, Kacper Jankowski, Olesia Lisowa, Andrzej Plucienniczak, Hilary Koprowski, Andrzej B. Legocki

Nasal administration of Schistosoma Mansoni egg antigens­cholera toxin B subunit conjugate to infected mice reduces immunopathology and mortality ................................ 305

Jia-Bin Sun, Bin-Ling Li, Mekuria Lakew, Jean-Marie Grzych, Andre Capron, Cecil Czerkinsky and Jan Holmgren

Anti-influenza vaccination changes expression of CD45 isoforms on peripheral blood NK cells ofthe elderly............................. 311

Andrzej Mysliwski, Piotr Trzonkowski, Ewa Szmit, Anita Dobyszuk, Jolanta Mysliwska, Jerzy Foerster

TUMOR IMMUNOLOGY

Presence and functions of immune components in the tumor microenvironment. ...... ...... .... ... ..... ... ....... ..... .... ............ ... 317

Isaac P. Witz

Expression ofTCR ~ chain of tumor associated lymphocytes from malignant pleural effusions................................ ......... 325

Jan Sikora, Grzegorz Dworacki and Jan Zeromski

Cytochrome c is rapidly extruded from apoptotic cells and detectable in serum of anticancer-drug treated tumor patients.. ..... ...... .................. ...... ....... ........ ............. ... ... 331

Andrea Renz, ChristofBurek, Walter Mier, Malgorzata Mozoluk, Klaus Schulze-Osthoff and Marek Los

CD 56 (N-CAM) antigen and mRNA expression in human endocrine glands.......................... ................................. ... 335

Jan Zeromski, Renata Jenek, Zofia Niemir and Wlodzimierz Liebert

Contents xv

CANCER~UNOTHERAPY

Glycodendrimeric ligands of C-type lectin receptors as therapeutic agents in experimental cancer................. ............. 343

Miloslav Pospisil, Luca Vannucci, Anna Fiserova, Katherina Krausova, Ondrej Horvath, Vladimir Ki'en, Franco Mosca, Thisbe. K. Lindhorst and Karel Bezouska

Exosomes in cancer immunotherapy: preclinical data............ .... ... 349 F.Andre, M.Andersen, J. Wolfers, A. Lozier, G.Raposo, V.Serra,

C. Ruegg, C.Flament, E.Angevin, S.Amigorena and L. Zitvogel

Bone marrow dendritic cell-based anticancer vaccines.. ............ .... 355 Marie Indrova, Luis Mendoza, Milan Reinis, Vladimir Vonka, Michal

Smahel, Sarka Neme~kova, Tana Jandlova and Jan Bubenik

Dendritic cell-based vaccines for therapy of HPVl6-induced tumours........................................................................ 359

Jan Bubenik, Jana Simova, Vladimir Vonka, Michal Smahel, Romana Mikyskova and Luis Mendoza

Cytokine gene transfection for autologous and allogeneic melanoma vaccines.......................................................... 365

S Tocbyk, L Birchall, R Erlich, N Halanek, JK Orleans-Lindsay and A Dalgleish

Brain tumor treatment with IL-2 and IL-12 producing autologous cancer cell vaccines.............. ........................... ... 369

Szilvia Desaknai, Katalin Lumniczky, Egon J. Hidvegi, Hirofumi Hamada and Geza Safrany

IL-ll is a potent anti-melanoma factor............................. ........ 373 Hanna Dams-Kozlowska, Dariusz Izycki, Andrzej Mackiewicz

IL-6 and GM-CSF in tumor rejection model of renal cell cancer.................................................................... ...... 379

Piotr J. Wysocki, Dariusz W. Kowalczyk, Dariusz Izycki, Piotr Grabarczyk, Zbigniew K wias and Andrzej Mackiewicz

xvi

The effects of peri tumoral therapeutic vaccination with. IL-2-secreting cells on growth of MC38 colon tumours in mice, local NO production and sentinel lymph node cells

Contents

activation................................... .......................... .......... 385 Elzbieta Pajtasz-Piasecka, Halina Kusnierczyk, KrzysztofP. Krawczyk,

Egbert Piasecki, Martyna Elas and Czeslaw Radzikowski

Improving the retroviral vector (RV) systems for immunotherapy of cancer................................ .................. 389

Piotr Grabarczyk, Katarzyna Gryska, Piotr J. Wysocki, Dariusz IZycki and Andrzej Mackiewicz

Tumor immunity in mice immunized with fibroblasts transfected with tumor DNA................. ......... ... ...... ... ........ 393

Edwin F. De Zoeten, Donghua Li and Edward P. Cohen

T cell response to tumor antigens and its therapeutic use in cancer patients .............................. ~............................. .... 403

Giorgio Parmiani, Chiara Castelli, Piero Dalerba and Licia Rivoltini

Humoral responses to melanoma vaccine, genetically modified with interleukin 6 and soluble interleukin 6 receptor................ 411

Sergiusz Nawrocki, Maria Laciak, Dariusz IZycki, Katarzyna Gryska, Piotr J. Wysocki, Piotr Grabarczyk Aldona Karczewska, Aldona Kaczmarek, Pawel Murawa, Julian Malicki, Stefan Rose-John and Andrzej Mackiewicz

Clinical studies of human papilloma vaccines in cervical cancer .••••• 419 Malcolm Adams, Leszek Borysiewicz, Alison Fiander, Stephen Man,

Bharat Jasani, Hossein Navabi, Alan S Evans and Malcolm Mason

Flow cytometric cytotoxicity assay with GFP gene modified target cells..................................................................... 429

Dariusz IZycki, Katarzyna Gryska, Piotr Grabarczyk, Piotr J. Wysocki, Anna Jarosh'lska, Sergiusz Nawrocki Dariusz W. Kowalczyk and Andrzej Mackiewicz

Flow and video-imaging cytometry - usefulness of DNA ploidy measurements in diagnosis of malignant melanoma.................... 435

Janusz Skowronek, Zbigniew Karas, Roman M.Krenz, and Jerzy B. Warchol

Contents xvii

Author Index............................................................................................ 439

Subject Index ................................................................. . 441