For research use only. Not for use in diagnostic procedures.

Targeted Quantitative AnalysesQuality-controlled - Standardized - Reproducible

Metabolic Phenotyping ServicesProfound Metabolomics Expertise

Comprehensive Analytical Portfolio

Project Management

Short Turn-Around Times

Biostatistics &Data Interpretation

Analytical Portfolio

Metabolite Portfolio

Metabolomics is the omics technology dealing with the detection, identifi cation, and quantifi cation of metabolites. The obtained results most closely refl ect the phenotype of a biological system, and are therefore increasingly acknowledged in systems biology. Our Metabolomics Phenotyping Services enable a quality-controlled, highly reproducible, quantitative analysis across the metabolic spectrum. The expert services offer full support in every step of your project, including project management, statistical analysis, and biomedical data interpretation.

The complexity of the interplay between metabolites in a biological system, the infl uence of the metabolism on health and disease, and the impact of external or internal factors on the metabolome is increasingly understood. Altogether, our assays cover more than 1,000 metabolites from over 30 classes of metabolites:

Key Benefi tsFull Expert Support - from Study Design to Actionable Results

Key FeaturesComprehensive Coverage of Metabolic Network

Biostatistics & Data Interpretation• Established institutional knowledge• Profound expertise in biostatistics• Biomedical interpretation &

metabolic pathway visualization

Project Management• Support from study design to data interpretation• Experience with discovery and validation studies• Guidance in state-of-the-art sampling, storage,

and shipment

Comprehensive Analytical Portfolio• Best fi t for every research question• Experience with wide range of matrices• Customized assay development

Short Turn-Around Times• High-quality results typically < 12 weeks after

sample receipt• Expert analysis in specialized routine laboratory• Experience with (pre-analytical) sample handling

Small Molecules

• Alkaloids

• Amine oxides

• Amino acids

• Amino acid related

• Bile acids

• Biogenic amines

• Carbohydrates and related

• Carboxylic acids

• Cresols

• Eicosanoids

• Energy metabolism intermediates

• Fatty acids

• Hormones and related

• Indoles and derivatives

• Nucleobases and related

• Vitamins and cofactors

Lipids

• (Acyl-)Carnitines

• Phosphatidylcholines

• Lysophosphatidylcholines

• Phosphatidylethanolamines

• Lysophosphatidylethanolamines

• Phosphatidylglycerols

• Lysophosphatidylglycerols

• Phosphatidylserines

• Sphingomyelins

• Ceramides

• Dihydroceramides

• Hexosylceramides

• Dihexosylceramides

• Trihexosylceramides

• Cholesteryl esters

• Diglycerides

• Triglycerides

• Free (oxy-)sterols

We are experienced with sample handling of a wide range of biological matrices from various species:

Our Metabolomics Phenotyping Services allow for a customized selection of assays from two basic approaches:

• Targeted Profi ling: recommended for hypothesis-driven, longitudinal, early-, and late-stage research studies

• Specialized Targeted Assays: recommended for specifi c research areas and targeted metabolic pathway analysis

The 10 mass spectrometry-based assays offered by our Metabolic Phenotyping Services refl ect a wide range of (patho-)physiological processes of a biological system:

Selection of Analyzed Species and Biological Matrices

Broad Analytical Spectrum - Targeted & Quantitative Analysis

1) Additional assays under development2) For detailed information please refer to respective product brochure or list of metabolites

SpeciesHuman, mouse, rat, chicken, pig, sheep, cow, dog, primate, guinea pig, hamster, fi sh, C. elegans, E. coli, P. pastoris

Matrix

Body FluidsPlasma, serum, urine, whole blood, blister liquid, saliva, cerebrospinal fl uid (CSF), broncho-alveolar lavage fl uid (BALF), milk

Tissue Liver, kidney, brain, lung, prostate, ovary, breast, colon, pancreas, muscle, skin

Cell CultureCHO cells, cultivated cancer cells, inclusion bodies, media and supplements (soy and yeast hydrolysates), neuronal cells

Others Feces, dried blood/plasma spots, cecal content

Approach Assay Name1) Number & Class of Metabolites2)

Targeted Profi ling

MxP® Quant 500 Kit 107 small molecules & 523 lipids

AbsoluteIDQ® p400 HR Kit 43 small molecules & 365 lipids

AbsoluteIDQ® p180 Kit 43 small molecules & 145 lipids

Specialized Targeted Assays

AbsoluteIDQ® Stero17 Kit 17 steroid hormones

Biocrates® Bile Acids Kit 20 bile acids

Eicosanoid Assay 17 eicosanoids

Fatty Acid Assay (total / free) 31 / 32 fatty acids

Lipid Assay 326 lipids

Oxysterol Assay 18 free (oxy-) sterols

Energy Metabolism Assay 21 energy metabolism intermediates

Statistical Analysis & Biomedical Data Interpretation

Institutional Knowledge in Metabolomics and BeyondStudy Design & Project Management

For confi dent decision making comprehensive statistical analysis and subsequent biomedical interpretation of the analytical data is required. A customized data report is generated delivering actionable results derived from robust and reproducible metabolomics data. With our profound scientifi c expertise in biostatistics and metabolomics we offer three reporting options:

In metabolomics, an elaborate study design is crucial to obtain meaningful, actionable results. Over the years, we have gained profound expertise in handling a wide range of projects from different research areas including healthcare, pharmacology, epidemiology, bioprocessing, nutrition, and animal health. Therefore, we may provide you with full support in project management and guidance in:

• Selection of scientifi c question & most suitable metabolomics assay(s)

• Study design & feasibility

• Sample handling: collection, processing, storage, shipment

Data Only

• Raw data processing

• Quality control

• Data normalization

• List of metabolite sums and ratios* (incl. background information)

Statistical Package

• Data processing (e.g. handling of values < LOD, outlier detection, log transformation)

• Imputation of missing values (e.g. log spline imputation)

• MetaboINDICATOR™ * (i.e. calculation of metabolite sums and ratios incl. background information)

• Descriptive statistics (e.g. mean, median, SD, IQR)

• Univariate statistics - Signifi cance testing (e.g. p-values, false discovery rates) - Fold changes

• Multivariate statistics (e.g. PCA, PLSDA, cluster analysis)

• Data visualization (e.g. box plots, score plots, heatmaps)

DataInterpretation

• Pathway analysis

• Biochemical interpretation (e.g. consideration of specifi c metabolite sums and ratios)

• Literature research

* Currently available for MxP® Quant 500 only

For research use only. Not for use in diagnostic procedures.

Document Nr. 35 030, V02-2019Photos: Biocrates, Fotolia

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