Professor Veronica (NCBS/ DBS TIFR)

Indian Academy of Science Fellow since 1995

www.ncbs.res.in

Local and regulated organization of membrane components during stem cell differentiation

Satyajit Mayor National Centre for Biological Sciences (NCBS), Bangalore, India

in collaboration with

inSTEM, Bangalore

Joseph  Mathew    Maneesha  Inamdar  JNCASR  

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Joseph  Mathew

   Maneesha  Inam

dar  JNCA

SR  

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Day 1

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Day 15

Adipogenic  differen-a-on  (Oil  Red  O  Stain)  

Osteogenic  differen-a-on(Alizarin  Red  Stain)  

Human  MSCs-­‐CG020-­‐P6  cells  –  21  days  of  differen-a-on  

CONTROL  

Joseph  Mathew    And  inSTEM  

Interesting to look at Stem Cells!

Engler et al, 2006

Engler et al, 2006

Towards a new picture of membrane organization and why we want to look at Stem Cells

The role of an active actin cortex- experiments and theory

Organization of GPI- tethered proteins at the cell surface- nano-scale clusters

Input Microenvironment

Output: Differentiation

Stem Cell

Towards a new picture of membrane organization and why we want to look at Stem Cells

The role of an active actin cortex- experiments and theory

Organization of GPI- tethered proteins at the cell surface- nano-scale clusters

Input Microenvironment

Stem Cell Gene Expression

Output: Differentiation

Input Microenvironment

Stem Cell Gene Expression

Output: Differentiation

The cell surface: A living information filter

Fluid Mosaic- patchwork quilt

6 nm Created by P. Kinnunen (University of Helsinki, Finland); source Edidin, Nature Reviews MCB, 2003

Active actin membrane composite

Active:

Actively manufactured

Non-equilibrium principles

Composite of membrane and cytoskeleton

Living - regulated

Passive:

Pre-existing phase segregation

Equilibrium principles

Membrane lipid environment

Passively formed

Flip-flop: lipid flux: secretion: endocytosis

Lets’ look at Stem Cells?

Engler et al, 2006

Adipogenic differentiation (Oil Red O Stain)

Osteogenic differentiation (Alizarin Red Stain)

Human MSCs-CG020-P6 cells – 21 days of differentiation

CONTROL

Joseph Mathew And inSTEM

Adipocytes

Undifferentiated cells

Phenotypically non-differentiated cells -

A membrane metric

Stem Cells

Engler et al, 2006

People *Rajat Varma, NYU/NIH *Pranav Sharma, CSH

*Shefali Sabharanjak, B’lore *Samit Chatterjee, CSH, NY *Pranav Sharma, CSH, NY *Manjula Kalia, ICGEB, N. Delhi

NCBS/Wellcome Trust/DST & DBT/ JST-ICORP/NanoTech Council

Sameera Bilgrami Debanjan Goswami Subhashri Ghosh Riya Raghupathy Suvrajit Saha Ruma Chandran

Rahul Chadda Sudha Kumari

Rob Parton, (Australia), G.Krishnamoorthy, (TIFR, Mumbai) Aki Kusumi, (Japan) Ram Vishwakarma (NII, Delhi)

Membrane Organization Endocytosis

Madan Rao, (RRI/NCBS) G. Kripa, (RRI/NCBS) Abhishek Chaudhry (NCBS) *Sarasij RC, (RRI/NCBS)

H. Krishnamurthy, CIFF NCBS

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Joseph Mathew

Balaji R. Gayatri M Joseph T.

Maneesha Inamdar, JNCASR Jyotsna Dhawan, inSTEM