Prof. Tapati Chakraborti

Professor in Biochemistry

Academic Qualifications PhD

Telephone No. 033-25828750x293, 294; +91-9239403709

Fax No. 033-25828282

E-mail Id. tcbiochem@gmail.com; t_chakraborti@yahoo.com;t_chakra08@rediffmail.com

Address. Department of Biochemistry & Biophysics, University of Kalyani, Kalyani741235, W.B, India

Teaching Area. Biochemistry Immunology Cell Biology Cellular Signaling

Area of Interest

L.donovani serine proteases in host-parasite interactions and immune modulation of visceral leishmaniasis. Immunomodulation by chemotherapeutic agents against leishmaniasis. Regulation of pulmonary vascular reactivity under oxidant and Ca2+ signaling phenomena. Studies on the function of Pleckstrin Homology (PH) domains and other protein that regulates cell signaling and disease processes.

Education (Post Graduation onwards)

Sl. No Degree Institution Conferring Field(s) Year

1. MSc University of Calcutta Biochemistry 1985

2. Ph.D Jadavpur University Science 1994

Research Training/Experience Year Position Place Particulars of work done

1987-1993 Research Fellow (JRF &

SRF) C.S.I.R

Indian Institute of Chemical

Biology, (C.S.I.R.), Calcutta

700032, India

Pathogenesis of visceral leishmaniasis diseases, mechanisms

involved in host parasite interactions and serodiagnosis of

visceral leishmaniasis (VL) using immune complex as a

potential probe.

1993-1999 Research Associate,

C.S.I.R.

Department of Biochemistry

and Biophysics, University of

Kalyani, Kalyani 741235, West

Bengal.

Biochemical studies on the regulation of pulmonary vascular

reactivity under oxidant and Ca2+ signaling phenomena.

1999-2001 Postdoctoral Research

Associate (Supported by

the American Heart

Association)

Department of Neuroscience,

Brain Institute, University of

Florida, Gainesville, Florida

32610, USA

Studies on function of Pleckstrin Homology (PH) domains and

other protein that regulates cell signaling and disease

processes

2002(July)-

2014

Associate Professor in

Biochemistry

Department of Biochemistry

and Biophysics, University of

Kalyani, Kalyani 741235, West

Bengal.

Teaching & research field:

Teaching: Immunology, Basic molecular biology, Cellular

signaling, Protein folding, Physiology

Reesearch field:

i) Studies on L.donovani serine proteases in host-parasite

interactions and immune modulation.of visceral leishmaniasis.

ii) Immunomodulation by chemotherapeutic agents against

leishmaniasis.iii) Biochemical studies on the regulation of pulmonary

vascular reactivity under oxidant and Ca2+ signaling

phenomena.

2014 to till

date

Professor in Biochemistry Department of Biochemistry

and Biophysics, University of

Kalyani, Kalyani 741235, West

Bengal.

Research projects Undertaken

Title of Project Funding

Agency

Duration

1. Role of naturally occurring serine protease inhibitor(s) from potato tuber in themodulation of host cell signaling and cellular invasion by Leishmania donovani (AsPI).

2. Studies on Leishmania donovani secretory serine protease in host-parasiteinteractions and immunemodulation of visceral leishmaniasis (As PI).

3. Studies on naturally occurring serine protease inhibitor(s) from Coccinia grandis (L)Voigt in the modulation of host cell signaling and cellular invasion by Leishmaniadonovani (As PI).

4. Studies on signal transduction mechanisms associated with ONOO.- mediatedregulation of β-adrenergic response in pulmonary smooth muscle cells ( As Co-PI).

5. Studies on phoapholemman phosphorylation by protein kinase A and proteinkinase C in regulating [Ca2+]i by α1β1 and α2β1 isozymes of Na+/K+ ATPase andNa+/Ca2+ exchanger in caveolae (signalosomes) of pulmonary artery smooth musclecells (As Co-PI).

6. Study on the mechanism(s) by which thromboxane A2 inhibits β-adrenergicresponse in pulmonary artery smooth muscle cells (As Co-PI).

7. Intracellular protozoan parasite metalloprotease(s) from Leishmania donovani:Identification, purification, characterization and its biological significance in visceralleishmaniasis. (As PI).

DST, Govt. Of India

CSIR, New Delhi

DAE, Govt of India

DBT, Govt. of India

CSIR, Govt. of India

DAE, Govt of India

UGC, Govt of India

16.10.2014-31.10.2017

01.11.2012-31.10.2015

13.05.2013-12.05.2016

01.01.2010-31.03.2014

01.4.2012-31.03.2015

15.12.2011-31.03.2014

01.02.2009-31.07.2013

Research projects Undertaken

Title of Project Funding

Agency

Duration

8. Signal transduction mechanism associated with leukotriene D4-mediated activationof phospholipase A2 in pulmonary artery smooth muscle cells (As Co-PI).

9. Biochemical mechanisms of Ca2+ dynamics in pulmonary artery smooth muscle cellsunder endothelin-1 stimulation (As PI).

10. Identification, purification and characterization of Ca2+ dependent protease(s) andendogenous inhibitors in Indian Strains of Leishmania donovani (As PI).

11. Biochemical mechanisms of the regulation of Ca2+ dynamics in microsomes ofpulmonary artery smooth muscle cells under thromboxane stimulation (As Co-PI).

12. Thromboxane-mediated activation of PLA2 in pulmonary artery: Role of a pertussistoxin sensitive protein (As Co-PI).

13. Protein kinase C-mediated activation of phosoholipase A2 in pulmonary vascularsmooth muscle cells under oxidant stimulation: Role of cAMP, cGMP and pertussistoxin sensitive protein (As Co-PI).

CSIR, Govt. of India

ICMR, Govt. of India

CSIR, Govt. of India

DST Govt. of India

ICMR, Govt. of India

ICMR, Govt. of India

01.06.2006-31.05.2010

01.10.2006-30.09.2009

01.06.2006-31.05.2009

01.12.2007-30.11.2010

01.3.2007-28.02.2010

01.08.2002-30.09.2006

Publications

Total Publications- 75 Impact Points- 179.97 Citations - 1219

(a) Book Chapters :05

1. Proteases as Virulence Factors in Leishmania: Focus on Serine Proteases as Possible Therapeutic Targets. TChakraborti et al., Proteases in Health and Disease, Advances in Biochemistry in Health and Disease (Eds: SChakraborti and NS Dhalla; Springer Publisher, New York) (2013)

2. An Overview of Endoplasmic Reticulum Calpain System. T Chakraborti et al; In Proteases in Cellular Dysfunctions.Eds: S.Chakraborti and NS Dhalla; Springer, New York (2013)

3. Matrix Metalloprotease-2 in the Development and Progression of Cardiovascular Diseases. T Chakraborti et al; inProteases in Health and Disease. Eds: S Chakraborti and NS Dhalla; Springer, New York (2013)

4. Regulation of MT1-MMP activation and its relevance to disease processes. T Chakraborti et al; In Proteases inCellular Dysfunctions. Eds: S Chakraborti and NS Dhalla; Springer, New York (2013)

5. Ca2+ dynamics under oxidant stress in the cardiovascular system. T Chakraborti et al; In Molecular and CellularResponses to Stress. Vol. 2, Page 213-224. Eds: KB Storey and JM Storey. Elsevier Science, New York, 2001

(b) Review articles: 13

1. Vascular aneurysms: a perspective. Chakraborti S, Chowdhury A, Alam MN, Sarkar J, Mandal A, Pramanik A, Chakraborti T. Indian JBiochem Biophys. 2014; 51(6):449-56. (Impact factor 1.073)

2. Protease Inhibitors in Potential Drug Development for Leishmaniasis. P Das, MdN Alam, D Paik, K Karmakar, T De, T Chakraborti.Indian J Biochem Biophys (2013) 50(5)363-376. (Impact factor 1.073)

3. Implications of calpains in health and diseases. Chakraborti S, Alam MN, Paik D, Shaikh S, Chakraborti T. Indian J Biochem Biophys.2012, 49(5):316-28. (Impact factor 1.073)

4. Mitochondrial calpain system: an overview. Kar P, Samanta K, Shaikh S, Chowdhury A, Chakraborti T, Chakraborti S. Arch BiochemBiophys. 2010;495(1):1-7 (Impact factor 3.3).

5. Calcium signaling phenomena in heart diseases: a perspective. Chakraborti S, Das S, Kar P, Ghosh B, Samanta K, Kolley S, Ghosh S,Roy S, Chakraborti T. Mol Cell Biochem. 2007; 298(1-2):1-40. (Impact factor 2.3)

6. Regulation of matrix metalloproteinases: an overview. Chakraborti S, Mandal M, Das S, Mandal A, Chakraborti T. Mol CellBiochem. 2003; 253(1-2):269-85. (Impact factor 2.3)

7. Structure and evolutionary aspects of matrix metalloproteinases: a brief overview. Das S, Mandal M, Chakraborti T, Mandal A,Chakraborti S. Mol Cell Biochem. 2003, 253(1-2):31-40. (Impact factor 2.3)

8. Clinical implications of matrix metalloproteinases. Mandal M, Mandal A, Das S, Chakraborti T, Chakraborti, S. Mol Celll Biochem.2003, 252(1-2):305-29. (Impact factor 2.1)

9. Protective role of magnesium in cardiovascular diseases: a review. Chakraborti S, Chakraborti T, Mandal M, Mandal A, Das S,Ghosh S. Mol Cell Biochem. 2002, 238(1-2):163-79. (Impact factor 2.3)

10. Complement activation in heart diseases: Role of oxidants. T Chakraborti, A Mandal, M Mandal, S Das, S Chakraborti. CellularSignalling 12, 607-617, 2000 (Impact factor 4.4)

11. Oxidant, mitochondria and calcium: An overview. T Chakraborti, S Das, M Mandal, S Chakraborti. Cellular Signalling 11, 77-85,1999 (Impact factor 4.4)

12. Oxidant-mediated activation of mitogen activated protein kinase and nuclear transcription factors in the cardiovascular system: Anoverview. S Chakraborti, T Chakraborti. Cellular Signalling. 10, 675-683, 1998 (Impact factor 4.2)

13. Targets of oxidative stress in the cardiovascular system: An overview. T Chakraborti, SKGhosh, JR Michael, SK Batabyal, SChakraborti. Mol Cell Biochem. 187, 1-10, 1998. (Impact factor 2.3)

(c) Original Research Papers: 66

2015

1. Cross-talk between p(38)MAPK and G iα in regulating cPLA 2 activity by ET-1 in pulmonary smooth muscle cells. Chakraborti S,Chowdhury A, Chakraborti T. Mol Cell Biochem. 2015; 400(1-2):107-23. (Impact factor 2.3)

2. Effect of different serine protease inhibitors in validating the 115 kDa Leishmania donovani secretory serine protease aschemotherapeutic target. Chakraborti T, Das P, Choudhury R, De T,. Indian J Biochem Biophys. 2015; 52(1): 14-22. (Impactfactor 1.073)

2014

3. Activation of proMMP-2 by U46619 occurs via involvement of p(38)MAPK-NFκB-MT1MMP signaling pathway in pulmonaryartery smooth muscle cells. Chowdhury A, Roy S, Chakraborti T, Dey K, Chakraborti S. Mol Cell Biochem. 2014; 385(1-2):53-68.(Impact factor 2.3)

4. Identification, purification and partial characterization of low molecular weight protein inhibitor of Na⁺/K⁺-ATPase frompulmonary artery smooth muscle cells. Rahaman SM, Dey K, Das P, Roy S, Chakraborti T, Chakraborti S. Mol Cell Biochem.2014; 393(1-2):309-17. (Impact factor 2.3)

5. In vitro anti-leishmanial efficacy of potato tuber extract (PTEx): leishmanial serine protease(s) as putative target. Paik D, Das P,De T, Chakraborti T. Exp Parasitol. 2014; 146:11-9. (Impact factor 1.8)

6. TLR mediated GSK3β activation suppresses CREB mediated IL-10 production to induce a protective immune response againstmurine visceral leishmaniasis. Paul J, Naskar K, Chowdhury S, Chakraborti T, De T. Biochimie. 2014; 107 Pt B:235-46. (Impactfactor 3.2)

7. TLR4-mediated activation of MyD88 signaling induces protective immune response and IL-10 down-regulation in Leishmaniadonovani infection. Paul J, Naskar K, Chowdhury S, Alam MN, Chakraborti T, De T. Indian J Biochem Biophys. 2014; 51(6):531-41. (Impact factor 1.073)

8. Leishmania donovani secretory serine protease alters macrophage inflammatory response via COX-2 mediated PGE-2production. Das P, De T, Chakraborti T. Indian J Biochem Biophys. 2014; 51(6):542-51. (Impact factor 1.073)

2013

9. Role of PKC-ζ in NADPH oxidase-PKCα-Giα axis dependent inhibition of β-adrenergic response by U46619 in pulmonary arterysmooth muscle cells.Chakraborti S, Roy S, Mandal A, Chowdhury A, Chakraborti T. Arch Biochem Biophys. 2013;540(1-2):133-44. (Impact factor 3.3).

2013

10. Role of phospholemman and the 70 kDa inhibitor protein in regulating Na+/K+ ATPase activity in pulmonary artery smoothmuscle cells under U46619 stimulation. Dey K, Rahaman SM, Chakraborti T, Chakraborti S. FEBS Lett. 2013 ;587(21):3535-40.(Impact factor 3.5)

11. Role of PKC-α in NF-κB-MT1-MMP-mediated activation of proMMP-2 by TNF-α in pulmonary artery smooth muscle cells. Roy S,Chakraborti T, Chowdhury A, Chakraborti S. J Biochem. 2013;153(3):289-302. (Impact factor 2.3)

12. Role of PKCα-p38 MAPK-Giα axis in peroxynitrite-mediated inhibition of β-adrenergic response in pulmonary artery smoothmuscle cells. Chakraborti S, Roy S, Chowdhury A, Mandal A, Chakraborti T. Cell Signalling 2013;25(2):512-26. (Impact factor 4.4)

13. Effect of m-calpain in PKCα-mediated proliferation of pulmonary artery smooth muscle cells by low dose of ouabain S Shaikh, JSarkar, A Pramanik, K Karmakar, S Chakraborti. Indian J Biochem. Biophys. 2013; 50, 419-427. (Impact factor 1.073)

2012

14. Role of PKCα-p38MAPK-Giα axis in NADPH oxidase derived O2.- -mediated activation of cPLA2 under U46619 stimulation inpulmonary artery smooth muscle cells. S Chakraborti, S Roy, A Mandal, K Dey, A Chowdhury, S Shaikh, T Chakraborti. ArchBiochem Biophys. 523:169-80 (Impact factor: 3.0).

2011

15. Role of TGF-β1 and TNF-α in IL-1β mediated activation of proMMP-9 in pulmonary artery smooth muscle cells: Involvement of anaprotinin sensitive protease. S Roy, K Samanta, T Chakraborti, A Chowdhury, S Chakraborti. Arch Biochem Biophys 22: 61-69.(Impact factor 3.0)

16. Role of protein kinase C-α in leukotriene D4-mediated stimulation of cytosolic phospholipase A2 in pulmonary artery smoothmuscle cells. S Chakraborti, T Chakraborti, Md Nur Alam, S Shaikh, S Roy. IIOAB (Special issue on redox signaling. vol: 2(5)(September Issue). (Impact factor 1.72)

2010

17. Immunolocalization and characterization of two novel proteases in Leishmania donovani: putative roles in host invasion andparasite development. R Choudhury, P Das, T De, T Chakraborti. Biochimie. 92:1274-86. (Impact factor 3.2)

18. In situ immunolocalization and stage-dependent expression of a secretory serine protease in Leishmania donovani and its role asa vaccine candidate. R Choudhury, P Das, SK Bhaumik, T.De, T Chakraborti. Clin Vaccine Immunol. 17:660-7. (Impact factor 2.5)

2010

19. m-Calpain-mediated cleavage of Na+/Ca2+ exchanger-1 in caveolae vesicles isolated from pulmonary artery smooth muscle. SShaikh, K Samanta, P Kar, S Roy, T Chakraborti, S Chakraborti. Mol Cell Biochem. 341:167-80. (Impact factor 2.2)

20. Characteristic properties of endoplasmic reticulum membrane m-calpain, calpastatin and lumen m-calpain: a comparativestudy between membrane and lumen m-calpains. K Samanta, P Kar, T Chakraborti, S Shaikh, Chakraborti S. J Biochem.147:765-79. (Impact factor 2.0)

21. Identification, purification and partial characterization of a 70kDa inhibitor protein of Na+/K+-ATPase from cytosol ofpulmonary artery smooth muscle. K Dey, T Chakraborti, S Roy, B Ghosh, P Kar, S Chakraborti. Life Sci. 27; 86:473-81. (Impactfactor 2.6).

22. Calcium-dependent cleavage of the Na+/Ca2+ exchanger by m-calpain in isolated endoplasmic reticulum. K Samanta , P Kar, TChakraborti, S Chakraborti. J Biochem. 147:225-35. (Impact factor 2.0).

2009

23. Role of protein kinase C in NADPH oxidase derived O2-.-mediated regulation of KV-LVOCC axis under U46619 induced increasein [Ca2+]i in pulmonary smooth muscle cells. S Chakraborti , A Chowdhury, P Kar, P Das , S Shaikh, S Roy , T Chakraborti . ArchBiochem Biophys. 487:123-30. (Impact factor 3.0)

24. Ca2+ influx mechanisms in caveolae vesicles of pulmonary smooth muscle plasma membrane under inhibition of alpha2beta1isozyme of Na+/K+-ATPase by ouabain. B Ghosh, P Kar, A Mandal, K Dey, T Chakraborti, S Chakraborti. Life Sci. 84:139-48.(Impact factor 2.6).

25. Identification, purification, and characterization of a secretory serine protease in an Indian strain of Leishmania donovani. RChoudhury, SK Bhaumik , T De, T Chakraborti. Mol Cell Biochem. 320:1-14. (Impact factor 2.168).

2008

26. Submitochondrial localization of associated mu-calpain and calpastatin. P Kar, T Chakraborti, K Samanta, S Chakraborti. ArchBiochem Biophys. 470:176-86. (Impact factor 3.0).

2007

27. Identification of calpastatin and mu-calpain and studies of their association in pulmonary smooth muscle mitochondria. P Kar,T Chakraborti, S Roy, R Choudhury, S Chakraborti. Arch Biochem Biophys. 466:290-9. (Impact factor 3.0).

28. Calcium signaling phenomena in heart diseases: a perspective. S Chakraborti, S Das, P Kar, B Ghosh, K Samanta, S Kolley, SGhosh, S Roy, T Chakraborti . Mol Cell Biochem. 298:1-40. (Impact factor 2.1)

2006

29. Solubilization, purification and reconstitution of Ca2+ATPase from bovine pulmonary artery sdmooth muscle microsomes bydifferent detergents: Preservation of native structure and function of the enzyme by DHPC. A Mandal, S Das, T Chakraborti, PKar, B Ghosh, S Chakraborti. Biochim Biophys Acta 1760: 20-31 (Impact factor; 3.0)

2005

30. Proteolytic activation of protein kinase Cin peroxynitrite-mediated activation of cytosolic phospholipase A2 in pulmonaryendothelium: Involvement of a pertussis toxin sensitive protein. T Chakraborti, S Das, S Chakraborti. Biochemistry (AmericanChemical Society) 44: 5246-5257. (Impact factor 3.7)

31. Role of MMP-2 in inhibiting Na+ dependent Ca2+ uptake by H2O2 in microsomes isolated from pulmonary smooth muscle. AMandal, T Chakraborti, R Choudhury, B Ghosh, AN Ghosh, S Das, S Chakraborti. Mol Cell Biochem. (Springer) 270: 79-87.(Impact factor 2.1 )

32. Role of MMP-2 in oxidant-mediated regulation of Ca2+ uptake in microsomes of bovine pulmonary artery smooth muscle. AMandal, T Chakraborti, R Choudhury, B Ghosh, S Chakraborti. Ind J Biochem Biophys. 42: 19-27. (Impact factor 1.073)

2004

33. Inhibition of Na+/Ca2+ exchanger by peroxynitrite in microsomes of pulmonary smooth muscle: Role of matrixmetalloproteinase-2. S Chakraborti, A Mandal, S Das, T Chakraborti. Biochim Biophys Acta 1671: 70-78. (Impact factor 3.0)

34. Isolation of MMP-2 from MMP-2/TIMP-2 complex: characterization of the complex and the free enzyme in pulmonary vascularsmooth muscle plasma membrane. S Das, A Mandal, M Mandal, T Chakraborti, S Chakraborti. Biochim Biophys Acta 1674:158– 174. (Impact factor 3.0)

35. Identification, purification and characterization of matrix metalloproteinase-2 in bovine pulmonary artery smooth muscleplasma membrane. S Das, M Mandal, A Mandal, T Chakraborti, S Chakraborti. Mol Cell Biochem. 258, 73-89. (Impact factor2.1)

36. Matrix metalloproteinase-2 mediated inhibition of Na+ dependent Ca2+ uptake by superoxide radical (O2.-) in microsomes ofpulmonary smooth muscle. A Mandal, B Ghosh, T Chakraborti, S Das, A NGhosh, S Chakraborti. IUBMB Life 56: 267 – 276.(Impact factor 3.514)

37. Role of aprotinin sensitive protease in protein kinase C-mediated activation of cytosolic phospholipase A2 by calciumionophore (A23187) in pulmonary endothelium. S Chakraborti, JR Michael, T Chakraborti. Cellular Signaling 16: 751-762.(Impact factor 4.011)

2003

38. Role of the matrix metalloproteinase-2 in the oxidant activation of Ca2+ATPase by hydrogen peroxide in pulmonary vascularsmooth muscle plasma membrane. M Mandal, S Das, T Chakraborti, A Mandal, S Chakraborti. J Biosci. 28: 205-213. (Impactfactor 1.88 )

39. Identification, purification and partial characterization of tissue inhibitor of matrix metalloproteinase-1 (TIMP-1) in bovinepulmonary artery smooth muscle. M Mandal, S Das, T Chakraborti, A Mandal, S Chakraborti. Mol Cell Biochem. 254: 145-155.(Impact factor 2.1)

40. Regulation of matrix metalloproteinases: An overview. S Chakraborti, M Mandal, S Das, A Mandal, T Chakraborti. Mol CellBiochem. 253: 269-285. (Impact factor 2.1)

41. Identification, purification and partial characterization of tissue inhibitor of matrix metalloproteinase-2 (TIMP-2) in bovinepulmonary artery smooth muscle. M Mandal, A Mandal, S Das, T Chakraborti, S Chakraborti. Mol Cell Biochem. 254: 275-287.(Impact factor 2.1)

42. Immune complex antigens as a tool in serodiagnosis of kala-azar. T Chakraborti, D Sarkar and DK Ghosh. Mol Cell Biochem.253, 191-198, 2003. (Impact factor 2.1)

2002

43. Role of Ca2+ dependent matrix metalloprotease-2 in stimulating Ca2+ATPase activity under peroxynitrite treatment in bovinepulmonary artery smooth muscle membrane. T Chakraborti, S Das, M Mandal, A Mandal and S Chakraborti. IUBMB Life 53: 1-7. (Impact factor 3.514 )

44. Role of a membrane associated Ca2+ dependent matrix metalloprotease in the activation of Ca2+ATPase by tertiarybutylhydroperoxide. S Das, T Chakraborti, M Mandal, A Mandal and S Chakraborti. Mol Cell Biochem. 237: 85-93. (Impactfactor 2.1)

45. Matrix metalloprotease 2-mediated activation of Ca2+-ATPase by superoxide radical (O2.-) in plasma membrane of bovinepulmonary vascular smooth muscle. M Mandal, T Chakraborti, S Das, A Mandal and S Chakraborti. Indian J Biochem. Biophys.39: 390-396. (Impact factor 1.073)

2001

46. Four casein kinase I isoforms are differentially partitioned between nucleus and cytoplasm. Z Fu, T Chakraborti, S, Morse, GSBennett, G Shaw. Exp Cell Res, 269: 275-86. (Impact factor 3.8 )

1999

47. Age-dependent change in arachidonic acid metabolic capacity in rat alveolar macrophages. T Chakraborti, M Mandal, S Dasand S Chakraborti. Biochem Molecular Biol International. 47: 501-507. (Impact factor 0.925)

48. Hypothalamo-pituitary-thyroid axis status of humans during development and ageing process. S Chakraborti, T Chakraborti, SDas, M Mandal and SK Batabyal. Clin Chim Acta 288, 137-145. (Impact factor 2.661)

1998

49. Oxidant-mediated activation of mitogen activated protein kinases and nuclear transcription factors in the cardiovascularsystem: An overview. S Chakraborti, T Chakraborti. Cellular Signaling 10: 675-683. (Impact factor 4.4)

1996

50. Identification and characterization of a parasite antigen in the circulating immune complexes of Indian kala-azar patient sera. TChakraborti, D Sarkar, DK Ghosh. In Indian Kala-azar (edited by S. Sunder), published by Kala-azar Medical Research Center,Department of Medicine, Institute of Medical Sciences, Banaras Hindu University, Varanasi, India, p 205-209

51. Role of an aprotinin sensitive protease in the stimulation of Ca2+ATPase by superoxide radical in microsomes of pulmonarysmooth muscle. T Chakraborti, SK Ghosh, JR Michael and SChakraborti. Biochem J. 317: 885-890. (Impact factor 4.4)

52. Redox state of pyridine nucleotides, but not glutathione, regulates Ca2+ release by H2O2 from mitochondria of pulmonarysmooth muscle. S Roychoudhury, SK Ghosh, JJ Ghosh, T Chakraborti and S Chakraborti.: Indian J Biochem Biophys. 33, 218-222.(Impact factor 1.073)

53. Role of hydroxyl radical in the oxidant H2O2-mediated Ca2+ release from pulmonary smooth muscle mitochondria. SRoychoudhury, SK Ghosh, T Chakraborti and S Chakraborti. Mol Cell Biochem. 159: 95-103. (Impact factor 2.1)

54. Role of hydroxyl radical in superoxide caused microsomal lipid peroxidation: Protective effect of anion channel blocker. SKGhosh, T Chakraborti, AB Banerjee, S Roychoudhury and S Chakraborti. J Biosci. 22: 35-43 1996. (Impact factor1.88)

55. Protective role of anion blocker in lipid peroxidation caused by H2O2 in microsomes of bovine pulmonary arterial smoothmuscle tissue. SK Ghosh, S.Roychoudhury, S.Kar, JJGhosh, T Chakraborti, S Chakraborti. Indian J Biochem Biophys. 33: 57-61.(Impact factor 1.073)

56. H2O2-induced lipid peroxidation in mitochondria of pulmonary smooth muscle and its modification by DFO, DMTU and DIDS. SRoychoudhury, T Chakraborti, SK Ghosh and S Chakraborti. Indian J Expt Biol 34: 1224-1228. (Impact factor 1.295)

57. Oxidant-mediated proteolytic activation of Ca2+ATPase in microsomes of pulmonary smooth muscle. SK Ghosh, T Chakraborti,JR Michael and S Chakraborti. FEBS Lett, 387: 171-174. (Impact factor 3.330)

1996

58. Involvement of a protease in tert-butylhydroperoxide-mediated activation of Ca2+ATPase in microsomes of pulmonarysmooth muscle. SK Ghosh, T Chakraborti, W.Selvamurthy, S Roychoudhury and S Chakraborti. Indian J Biochem Biophys. 33:414-419. (Impact factor 1.073)

1995

59. Down regulation of protein kinase C attenuates the oxidant hydrogen peroxide caused stimulation of phospholipase A2 inpulmonary smooth muscle cells. S Chakraborti, T Chakraborti. Cellular Signaling, 7: 75-83. (Impact factor 4.011)

60. Role of hydroxyl radical in the stimulation of arachidonic acid release caused by H2O2: Protective effect of anion channelblocker. S Chakraborti, SK Batabyal and T Chakraborti. Mol Cell Biochem. 146: 91-98. (Impact factor 2.1)

61. Expression of antigens in virulent and avirulent Indian strains of Leishmania donovani. T Sanyal (Chakraborti), P Ganguly, DKGhosh and D Sarkar. J Bioscience 19: 291-299. (Impact factor 1.88)

62. Immunoblotting identifies an antigen recognized by antigp63 in the immune complex of Indian kala-azar patients. T Sanyal(Chakraborti), D.K.Ghosh and D Sarkar. Mol Cell Biochem. 130: 11-17. (Impact factor 2.1)

1994

63. Role of membrane associated serine esterase in the activation of phospholipase A2 by calcium ionophore (A23187). SChakraborti, SK Batabyal, JR Michael and T Chakraborti. Mol Cell Biochem. 130: 121-128. (Impact factor 2.1)

1992

64. Defining the role of protein kinase C in calcium ionophore (A23187)mediated activation of phospholipase A2 in pulmonaryendothelium. S Chakraborti, JR Michael, T Sanyal. Eur J Biochem. 206: 967-972. (Impact factor 3.79)

1991

65. Identification of immune complex antigens in sera of Indian Kala-azar patients. T Sanyal (Chakraborti), DK Ghosh and DSarkar. Indian J Expt Biol. 27: 411-415. (Impact factor 1.295)

1989

66. Evaluation of antileishmanial activity of trans-aconitic acid. S. Mishra, T Sanyal (Chakraborti), D. Sarkar, PK Bhattacharyyaand DK Ghosh. Biochemical Medicine and Metabolic Biology, 42: 171-178. (Impact factor 2.595)

1. Invited lecture: In vitro Antileishmanial Efficacy of Plant-Derived Small Antimicrobial Peptide (sAMP) Against Antimonial-Resistant(SbR) Leishmania donovani at international Conference on Molecular Biology & Its applications: Organized by Department of lifescience and Biotechnology, Jadavpur Unversity, Kolkata, on 14th & 15th February, 2014

2. Invited lecture: Endogenous serine protease inhibitor(s) as prophylaxis against visceral leishmaniasis: An approach at “Researchworkshop on Inborn errors of metabolism & Metabolic Disorders” Organized by College of Medicine & JNM Hospital, WBUHS, 7th &8th March, 2013

3. Efficacy of natural inhibitory components from potato tubers targeting Leishmania donovani. at “Research workshop on Inbornerrors of metabolism & Metabolic Disorders” Organized by College of Medicine & JNM Hospital, WBUHS, 7th & 8th March, 2013

4. Leishmania donovani intracellular serine protease (SP-Ld) exacerbates infection in macrophages through COX-2 dependent Pro MMP-9/MMP-9 at ‘Research workshop on Inborn errors of metabolism & Metabolic Disorders” Organized by College of Medicine & JNMHospital, WBUHS, 7th & 8th March, 2013

5. “Leishmania donovani INTRACELLULAR SERINE PROTEASE: PURIFICATION, CHARACTERIZATION, IMMUNOLOCALIZATION ANDPUTATIVE ROLES IN HOST INVASION” at the 2nd International Conference on “Perspectives of Cell Signaling & Molecular Medicine”at Bose Institute, Kolkata, West Bengal, India January 8-11, 2012

6. Differential Expression of Intracellular Serine Protease in Leishmania donovani: Putative roles in immunopathogenesis” at ‘100 yearsof Antimonial Drug’ Organized at IICB, Kolkata, WB, India, 30th November to 1st December, 2012.

7. Antileishmanial Activity of Potato Serine Protease Inhibitors (PSPIs): Leishmanial Serine Protease as Drug Target” at ‘100 years ofAntimonial Drug’ Organized at IICB, Kolkata, WB, India , 30th November to 1st December, 2012.

8. Partial Purification and Characterization of a Novel Endogeneous Serine Protease Inhibitor from Indian Strain of Leishmania donovaniat ‘100 years of Antimonial Drug’ Organized at IICB, Kolkata, WB, India 30th November to 1st December, 2012.

9. Ca2+ influx mechanisms in caveolae vesicles of pulmonary smooth muscle plasma membrane under inhibition of α2β1 isozyme ofNa+/K+ATPase by ouabain at International conference on “Perspectives of Cell signaling and Molecular Medicines” Bose Institute,Kolkata, on 27-29 Nov., 2008

Presented papers/posters at International/national conferences

A. International

1. “In vitro Antileishmanial Efficacy of Plant-Derived Small Antimicrobial Peptide (sAMP) Against Antimonial-Resistant (SbR) Leishmaniadonovani”. National Conference on Current Research in Microbiology organized by Department of Botany, University of Calcutta onsuccessful completion of its 100 years, in collaboration with Prof A. K. Chandra Memorial Committee, Kolkata on 3-4th April 2014.

2. “Antileishmanial activity of serine protease inhibitor rich plant leaf extract (LEx-SPI) against Indian strain of Leishmania donovani”.National Conference on Current Research in Microbiology organized by Department of Botany, University of Calcutta on successfulcompletion of its 100 years, in collaboration with Prof A. K. Chandra Memorial Committee, Kolkata on 3-4th April 2014.

3. “National Conference on CME in Immunology” organized by Dept. of Zoology, West Bengal State University, Barasat and IndianImmunology Society, New Delhi during. November 4-5, 2011.

4. Seminar on “Frontier in Molecular Biology and Workshop on Basic Flowcytometry” organized by Dept. of Zoology, The University of Burdwan, Burdwan, West Bengal, India, 9th July, 2012

5. “Purification, localization, and stage dependent expression of a secretory serine protease in Indian strain of Leishmania donovani andits putative role as a vaccine candidate” In 22nd National Congress of Parasitology Organized by Dept of Zoology, University of Kalyani,Zoological Survey of India, Indian Society of Parasitology 30th October-1st - November, 2010

6. Invited lecture entitled: Identification of antigens with potential for immunodiagnosis of visceral leishmaniasis. Seminar on Recenttrends in Molecular Physiology University of Kalyani, 9th Feb., 7. As resource person Refresher’s Course on Biophysics. Topic: Cellular,Molecular and structural biology” UGC, New Delhi, Dept of Biochemistry, University of Kalyani, 7th -28th January, 2003

7. “Efficacy of DHPC over TritonX100 and C12E8 in the solubilization, purification and reconstitution of smooth muscle mircosomalCa2+ATPase”. at National symposium on Molecular mechanisms of diseases and Drugs Design held, Saha Institute of Nuclear Physics,Kolkata, on 16-18 November, 2005

8. “Identification, purification and partial characterization of 1, 10-phenanthroline sensitive protease from India strains of kala-azar” at18th National Congress of Parasitology. Topic: “Advances in Parasitology Research of Tropical Diseases” held on 24th November, 2006

9. Environmental aspects of microbes in the New Millenium as Chairperson of the Seminar organized by Rastroguru SurendranathCollege, Barrackpur, West Bengal held on 24-25th Feb, 2006

B. National

Research Guidance

PhD Awarded : 02; Thesis submitted: 02 Students working : 07

Name and present position of the PhD awarded candidates:

I. Dr. Rajdeep Choudhury:- Post Doctoral Fellow, Evolutionary Medicine Unit, Dept. of Molecular Medicine & Haematology, University of the Witwatersrand, Johannesburg, SA

II. Dr. Pulak Kar:- Research Associate, Department Physiology, Anatomy and Genetics, Oxford University, U.K.

Awards/Honour/Achievements

a) Recipient of Research Associate-ship from the American Heart Association for research in the Departmentof Neuroscience, Brain Institute, University of Florida, Gainesville, Florida, USA of Neuroscience (1999-2001).

b) Research Associate, University of Kalyani, Indian Council of Medical Research & Council of Scientific &Industrial Research (CSIR) (1993-1999).

c) Senior Research Fellow, Indian Institute of Chemical Biology (CSIR), Kolkata, CSIR (1989 – 1993).d) Junior Research Fellow, Indian Institute of Chemical Biology (CSIR), Indian Council of Medical Research

(1987-1989).e) Most cited paper (Scopus Search) from the Universities in West Bengal in the subjects: Biochemistry,

Molecular Biology, Genetics : Regulation of matrix metalloproteinases: an overview. Chakraborti S, Mandal

M, Das S, Mandal A, Chakraborti T. Mol Cell Biochem (2003) 253:269-85.