prof. oladapo ashiru m.b.b.s, ms, phd. hcld/cc(abb-usa), fasn, fnsem, ofr developments and landmarks...
TRANSCRIPT
![Page 1: PROF. OLADAPO ASHIRU M.B.B.S, MS, PHD. HCLD/CC(ABB-USA), FASN, FNSEM, OFR DEVELOPMENTS AND LANDMARKS OF GENETIC TESTING IN WEST AFRICA 48 th Annual General](https://reader035.vdocuments.us/reader035/viewer/2022062518/56649d845503460f94a6a55f/html5/thumbnails/1.jpg)
PROF. OLADAPO ASHIRUM.B.B.S, MS, PHD. HCLD/CC(ABB-USA), FASN, FNSEM, OFR
DEVELOPMENTS AND LANDMARKS OF GENETIC TESTING IN WEST
AFRICA
48th Annual General Meeting and Scientific Conference
SOGON ASABA 2014.
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ASSISTED REPRODUCTIVE TECHNOLOGY (ART)
Conception requiring the complex handling or manipulation of male and female gametes in-vitro to facilitate pregnancy
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LANDMARKS IN ART
1969 Sperm Capacitation Chang & Yamagimachi
1976 IVF in London Steptoe & Edwards 1978 IVF baby London Steptoe & Edwards
(First IVF Baby – Louise Brown) 1977 LHRH Schally & Guileman 1978 FSH +ve feedback Ashiru & Blake 1980 IVF in Melbourne, Australia Carl Wood 1982 IVF in Virginia Jones 1984 IVF in Lagos, Nigeria Ashiru & Giwa-
Osagie– 1986/1989
1992 ICSI Jacques Cohen
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LANDMARKS IN PGD
Jacobson CB & Barter RH 1960 - 1st prenatal diagnosis
Saiki et al 1985 – PCR Alan Handyside and Robert Winston 1989 –
Embryo Biopsy Verlinsky Y et al 1999 – Polar body testing
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LANDMARKS IN PGD
1990: Sexing Human Preimplantation Embryo, & Polar Body Analysis for Mendelian Disease; - First PGD Baby Born
1993: FISH Analysis for Sexing and for PGD of Aneuploidies
1996: PGD for Chromosomal Translocations 1999: PGD for Late Onset Common Disorders
with Genetic Predisposition
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2000: Pre-implantation HLA Typing
2002: 1st Thousand PGD Babies Born
2002 onwards: Many technological
developments CGH, aCGH, NGS, SNP,qPCR
LANDMARKS IN PGD
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TYPES OF PREIMPLANTATION GENETIC TESTING
Preimplantation Genetic Diagnosis (PGD)– Targeted genetic testing of known
genetic abnormality in the couple:Single gene disorderStructural chromosomal
abnormality
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Preimplantation Genetic Screening for Aneuploidy (PGS)– Screening for numerical abnormality of select
chromosomes: 13, 18, 21, X, Y, etc Preimplantation Gender Determination or
Gender Selection
TYPES OF PREIMPLANTATION GENETIC TESTING
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INDICATIONS FOR PGD
Autosomal dominant disorders– One of the couple is a carrier of the
genetic defect (e.g., Huntington Disease or dwarfism)
Autosomal recessive disorders:– Both couple are carriers of the genetic
defect (e.g., Sickle Cell Disease)
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INDICATIONS FOR PGD
X-linked disorder– One of the couple is a carrier of a x-linked genetic
defect (e.g., Hemophilia) Human leukocyte antigen (HLA) matching Structural chromosomal abnormality
– One of the couple is a carrier of a chromosome abnormality (translocation, inversion, deletion, insertion)
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INDICATIONS FOR PGS (ANEUPLOIDY SCREENING)
Advanced maternal age Recurrent pregnancy loss Repeated IVF failure Severe male factor infertility
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INDICATIONS FOR GENDER SELECTION
One of the couple is a carrier of x-link disorder
Family balancing
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• May be largely diagnostic• No paternal/meiosis II/post-zygotic information• May require later follow-up testing (d3/d5)
BIOPSY TECHNIQUES
• Accounts for meiosis II errors• No paternal/post-zygotic information• Most expensive option diagnostically
• Biopsy most detrimental• Chromosomal mosaicism common• Associated with Low implantation
• Highly recommended• 3-10 (?) cells (high accuracy/reliability)• Significance of mosaicism?• Least expensive per patient (diagnosis)• May require vitrification
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BIOPSY OBJECTIVE
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Methods of Genetic Analysis: PGD single gene disorder
– Whole genome amplification– Polymerase chain reaction (PCR)– Direct detection of specific mutation– Genotyping of linked polymorphic markers to
ensure: Bi-parental inheritance Infer the inheritance of normal copy of the gene (Allele)
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Methods of Genetic Analysis: Structural chromosomal abnormality
Fluorescent in situ hybridization (FISH)
PCR-based testing– Whole genome amplification– Genotyping of linked polymorphic markers
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METHODS
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FISH Gender Determination
Orange: X chromosomeGreen: Y chromosome
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FISH Aneuploidy Screening
Normal Male Normal Female
Trisomy 21 Male Red: chromosome 13Cyan: chromosome 18Green: chromosome 21Purple: chromosome XYellow: chromosome Y
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ARRAY COMPARATIVE GENOMIC HYBRIDIZATION (aCGH)
• A molecular cytogenetic technique
• Detects 'copy number changes' of chromosomes on a genome
• Embryo DNA is compared with a known normal DNA specimen utilising thousands of specific genetic markers
• More accurate results (fewer false normal or false abnormal results)
• Error rate of 2% compared to 5 - 10% of FISH
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aCGH versus Conventional CGH
• Better resolution (as low as 5 – 10 kilobytes of DNA sequences)
• Cloned DNA fragments with exact chromosomal location replaces reference metaphase spread
• Better detection of aberrations
• Better identification of micro deletions and duplications
• Increases the possibility of mapping changes directly onto the genomic sequence
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ARRAY COMPARATIVE GENOMIC HYBRIDIZATION (aCGH) - METHOD
Same principle as conventional CGH Labelling of DNAs (control and test) with 2 different
'flurophores' (green and red) Green flurophore (cyanine 3) for test / patient and red
flurophore (cyanine 5) for control / reference are used as 'probes'
Competitive cohybridisation of probes onto nuclei acid targets (cloned genomic fragments (BACs / plasmids), cDNAs or oligonucleotides) with thousands of genetic markers.
Digital imaging / specialised microscopes to capture and quantify fluorescence intensities of hybridised flurophores.
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ARRAY COMPARATIVE GENOMIC HYBRIDIZATION (aCGH) – RESULT
Interpretation of results. Ratio of the fluorescence intensities is proportional to the ratio
of 'copy number of DNA sequences' in the test and reference genomes.
Altered Cy 3 : Cy 5 ratio indicates a loss or gain of the patient DNA at that specific genomic region
If Cy 3 : Cy 5 ratio is equal on one probe (equal intensities of the flurophores), patient's genome is interpreted as having equal quantity of DNA as in the reference sample.
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Array CGH remains the ‘gold standard’ for PGS with the greatest clinical experience to date
RCTs show great promise for PGS Numerous RCTs are ongoing Relatively easy to set up Use of a reference laboratory recommended
as a first step
ARRAY COMPARATIVE GENOMIC HYBRIDIZATION (aCGH)
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DETECTION OF WHOLE CHROMOSOME ANEUPLOIDIES IN SINGLE CELLS
Trisomy 13 male(47,XY,+13)
Sequencing Results
24Sure Results
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PGD/PGS SINCE 2009 (MART DATA)
Chromosomes Aneuploidy (CA) Single gene disorders (Sickle cell – SC) Family balancing (sex selection – FB)
So far over 75 cases have been done
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SUMMARY OF REASONS FOR PGD/PGS (N=75)
CA FB SC0%
5%
10%
15%
20%
25%
30%
35%
40%
45%43%
32%23%
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SUMMARY FOR PGS (N= 32)
Normal Abnormal No Result
0%
10%
20%
30%
40%
50%
60%
26%
59%
15%
SUMMARY OF EMBRYO BIOPSED FOR CHROMOSOMES ANEUPLIODY (N=300)
59% of embryos biopsied were abnormal
15% No result rate
? Number and quality of cells tested
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SICKLE CELL DIAGNOSIS (PGD) N = 17
41% of biopsied embryos were homozygous and heterozygous normal
34% of embryos undiagnosed
Normal Abnormal No Result0%
5%
10%
15%
20%
25%
30%
35%
40%
45% 41%
25%
34%
SUMMARY OF EMBRO BIOPSED FOR SICKLE CELL DIAGNOSIS
(N= 133)
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FAMILY BALANCING – SEX SELECTION (N=26)
Male Total Female0%
10%
20%
30%
40%
50%
60%
70%
80%
90%
100%
47%
100%
47%
SUMMARY OF GENDER ANALYSIS IN FAMILY BALANCING
No difference in the proportion of male and female embryos
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SUMMARY OF PREGNANCY RATE
CA FB SC0%
10%
20%
30%
40%
50%
60%
70%
32%
59%
9%
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SUMMARY OF CLINICAL PREGANCY RATE
CA (%) FB (%) SS (%)0
5
10
15
20
25
30
35
30
35
9
Clinical Rate Series2
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SETTING UP A PGD LAB
Two ways
IVF centre and PGD centre in the same institute – preferred
Transport PGD
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ORGANIZATION OF THE PGS/PGD CENTRE
Highly successful IVF unit Patients need genetic and specific PGD counselling Biopsy performed by trained embryologist Diagnosis performed by molecular biologist/cytogeneticist Patient information leaflets and consents Excellent communication between IVF centre and
diagnosis lab Join the PGD Consortium
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WHAT MAKES A GOOD PGD CENTRE
COMMUNICATION
Excellent IVF Platform
Excellent Diagnostics Laboratory
Integration of Services
Commitment to Follow-up
TRANSPORT PGD
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FUTURE OF PGD/PGS
• PGD–New technology to allow diagnosis
of more disorders–Whole genome amplification–SNP microarrays, array-CGH,
quantitative real time PCR (qPCR)–Blastocyst biopsy and vitrification
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• PGS–Randomised controlled trials to see if valid
procedure with clinical significance–NOT cleavage stage biopsy, NOT FISH–Try polar body or trophectoderm biopsy–SNP, array-CGH, or sequencing
FUTURE OF PGD/PGS
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BEETHOVEN
“I RECOGNIZE NO SUPERIORITY IN MANKIND OTHER
THAN GOODNESS.”
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THE TEAM
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THANK YOU
FOR LISTENING!