Director, Dr. Mohamed L. Salem, PhD

Prof. of Immunology

Tanta University, Egypt

Center of Excellence in Cancer Research

(CECR)

Location

Tanta University Educational Hospital

Location

Established Date

June 2013 through a project funded by Science and

Technology development Fund (STDF)

PI: Prof. Mohamed Labib Salem

Affiliation: Tanta University

Established Date

Dr. Mohamed L.SalemProf. of immunology

Dr. Said HammadProf. of clinical Pathology

Dr. Mohamed RamadanProf. of Pediatric

Dr. Mohamed AttiaAssis. Prof. of Clinical Pathology

Dr. Abdel-Aziz ZidanPhD of Immunology

Ms. Mona Fouad ZidanResearcher Assistant

Research Team

Research Team

Organogram

Correlating immunologic and clinical performancebetween genomic, transcriptomic and proteomicprofiling of circulating tumor cells versus primarytumor cells

CECR Main Project

CECR Core Facility

Nomination:The BD FACSCanto derives its name from the musical term “bel canto” meaning

operatic singing that focuses on ease and purity of tone with an agile and precise

vocal technique.

Flow Cytometry Unit

• BD FACSCanto II Cytometer

• The BD FACSCanto system has a capacity for three lasers, eight-parameter

detection while capturing particle sizes from 0.5 to 50 μm in diameter.

• Digital electronics for processing up to 10,000 events per second, and a novel

sample injection tube supporting

Multiparameter detection helps in:

• Advanced cell subset analysis.

• Multiparameter functional studies.

• Soluble protein detection.

• Quantification studies for cell-surface antigens and soluble proteins.

• Decreases the total sample volume necessary for a specific project as more

parameters.

Specifications of the instrumentSpecifications of the instrument

• Immunophenotyping by flow cytometry is an important tool in the diagnosis and

staging of patients with a haematological neoplasm as well as solid tumors.

• Some of the flow cytometry applications in diagnosis of different diseases are listed

below:Detection of

minimal residual disease

BD FACSCanto Applications

Stem cell enumeration

Analysis of Leukemias and

Lymphomas

Reticulocyte analysis

Detection of Immunodeficiency

diseases

Detection of HIV infection

Analysis of different types of solid

tumors.

Detection of autoantibodies

Research & Clinical applications of the instrumentSpecifications of the instrument

The IsoFlux system enriches rare cells from biological samples and prepare them for further analysis , It gives immediate access to high-quality , viable cells in a high density format , ready for use in the application of your choice

Benefits:

• Access a wide range of samples.

• Use your own capture criteria.

• Capture intact, pure cells.

• Minimize Sample Diluation

• Save Time

• Save Space

• Utilize validated CTC analysis workflows.

Cell Sorting Unit

Cell Sorting Unit

IsoFlux Applications

Enumeration ImmunofluorescenceFluorescence in situ

hybridization

Next generation sequencing

Mutation detection Gene expression

Microarray

A high throughput technology that allows detection of thousands of genes simultaneously.

Features:

• High samples throughput.

• Laser excitation at 532nm, dual- laser excitation .

• Scanning pixel resolution 2µm

• Scan sequence: simultaneous scan and independent scan.

• Scan/read time: approximately 5 samples/hour.

Genomic & Proteomic Unit

Genomic & Proteomic Unit

Microarry Applications

TransiptomemRNA ExpressionmiRNA ExpressionSplice Variance

Genome StructureDNA Copy number

Genome VariationSingle Nucleotide Polymorphism

Copy number Variations

ProteomeProtein-Protein InteractionsDNA – Protein inreraction

EpigeneticsDNA Methylation

Chromatin Structure

• LumineX200

Genomic & Proteomic Unit

Flexible Multiplexing Technology

• Open Platform for Genomic & Proteomic assays

• Life Science Research & Diagnostic assays available.

• Broad menu of commercial assays and laboratory developed tests

• Multiplex up to 500 tests in one well of microliter plate.

• Decrease sample volume requirements.

• Reduce assay reagent volume, expense & labor.

• Generate more information on interrelationships between analytes within a single sample.

Genomic & Proteomic Unit

Genomic & Proteomic Unit

Luminex Applications

Clinical Diagnostics Clinical Research Life Science Research

Biosurveillance Assay Development Tools

CECR CollaboratorsCECR Collaborators

Monday 24 February 2014 The 1st Annual Conference of

The Center of Excellence in Cancer ResearchNext Generation Cancer Research & Diagnosis

In Collaboration withResearch Centers of Excellence

(STDF- Affiliated Centers)

CECR Conferences

CECR PhD. & MSc. Students

Thank You !