product code description€¦ · printing date 26.06.2015 revision: 22.06.2015 40.1.5 section 1:...

50
Kit components 06/26/2015 Product code Description 1665120 C. elegans Behavior Kit 1665120EDU 10030952 Components: 1665125 NGM Agar Lite 10040987 Agar 10030936 2.5 M NaCl in wash buffer 10030937 C. Elegans Wash Buffer 10X 10030881 E. Coli OP50-pBAD 10030938 Cholesterol in Alcohol 5 mg/ml 1660407 Ampicillin, lyophilized 1665122 C. elegans Wild / Mutant

Upload: others

Post on 27-Jun-2020

2 views

Category:

Documents


0 download

TRANSCRIPT

Page 1: Product code Description€¦ · Printing date 26.06.2015 Revision: 22.06.2015 40.1.5 SECTION 1: Identification of the substance/mixture and of the company/undertaking · 1.1 Product

Kit components06/26/2015

Product code Description

1665120 C. elegans Behavior Kit1665120EDU 10030952

Components:

1665125 NGM Agar Lite

10040987 Agar

10030936 2.5 M NaCl in wash buffer

10030937 C. Elegans Wash Buffer 10X

10030881 E. Coli OP50-pBAD

10030938 Cholesterol in Alcohol 5 mg/ml

1660407 Ampicillin, lyophilized

1665122 C. elegans Wild / Mutant

Page 2: Product code Description€¦ · Printing date 26.06.2015 Revision: 22.06.2015 40.1.5 SECTION 1: Identification of the substance/mixture and of the company/undertaking · 1.1 Product

Page 1/6

Safety data sheetaccording to 1907/2006/EC, Article 31

Printing date 26.06.2015 Revision: 22.06.2015

40.1.5

SECTION 1: Identification of the substance/mixture and of the company/undertaking

· 1.1 Product identifier

· Trade name: NGM Agar Lite

· Article number: 10030934· 1.2 Relevant identified uses of the substance or mixture and uses advised againstNo further relevant information available.

· Application of the substance / the mixture Laboratory chemicals

· 1.3 Details of the supplier of the safety data sheet· Manufacturer/Supplier:Bio-Rad Laboratories S.r.l.Via Cellini, 18A20090 Segrate-MilanoItalyphone: 39-02-216091 fax: [email protected] / [email protected]

· Information department:Technical services, customer [email protected] / [email protected]

· 1.4 Emergency telephone number: 02-216091 - active during office hours for technical information

* SECTION 2: Hazards identification

· 2.1 Classification of the substance or mixture· Classification according to Regulation (EC) No 1272/2008 The product is not classified according to the CLP regulation.

· 2.2 Label elements· Labelling according to Regulation (EC) No 1272/2008 Void· Hazard pictograms Void· Signal word Void· Hazard statements Void· 2.3 Other hazards· Results of PBT and vPvB assessment· PBT: Not applicable.· vPvB: Not applicable.

* SECTION 3: Composition/information on ingredients

· 3.2 Chemical characterisation: Mixtures· Description: Mixture of the substances listed below with nonhazardous additions.· Dangerous components:CAS: 7647-14-5EINECS: 231-598-3

sodium chloride Skin Irrit. 2, H315; Eye Irrit. 2, H319 5-10%

· Additional information For the wording of the listed risk phrases refer to section 16.

SECTION 4: First aid measures

· 4.1 Description of first aid measures· After inhalation Supply fresh air; consult doctor in case of complaints.

(Contd. on page 2) IE

Page 3: Product code Description€¦ · Printing date 26.06.2015 Revision: 22.06.2015 40.1.5 SECTION 1: Identification of the substance/mixture and of the company/undertaking · 1.1 Product

Page 2/6

Safety data sheetaccording to 1907/2006/EC, Article 31

Printing date 26.06.2015 Revision: 22.06.2015

Trade name: NGM Agar Lite

(Contd. of page 1)

40.1.5

· After eye contact Rinse opened eye for several minutes under running water.· After swallowing Induce vomiting and call for medical help.· 4.2 Most important symptoms and effects, both acute and delayed No further relevant information available.· 4.3 Indication of any immediate medical attention and special treatment neededNo further relevant information available.

SECTION 5: Firefighting measures

· 5.1 Extinguishing media· Suitable extinguishing agentsCO2, extinguishing powder or water spray. Fight larger fires with water spray or alcohol resistant foam.

· 5.2 Special hazards arising from the substance or mixture No further relevant information available.· 5.3 Advice for firefighters· Protective equipment: No special measures required.

* SECTION 6: Accidental release measures

· 6.1 Personal precautions, protective equipment and emergency procedures Wear protective clothing.· 6.2 Environmental precautions: Do not allow to enter sewers/ surface or ground water.· 6.3 Methods and material for containment and cleaning up: Pick up mechanically.· 6.4 Reference to other sectionsNo dangerous substances are released.See Section 7 for information on safe handlingSee Section 8 for information on personal protection equipment.See Section 13 for disposal information.

SECTION 7: Handling and storage

· 7.1 Precautions for safe handling No special precautions are necessary if used correctly.· Information about protection against explosions and fires: No special measures required.

· 7.2 Conditions for safe storage, including any incompatibilities· Storage· Requirements to be met by storerooms and receptacles: According to product specification· Information about storage in one common storage facility: Not required.· Further information about storage conditions: None.· 7.3 Specific end use(s) No further relevant information available.

SECTION 8: Exposure controls/personal protection

· Additional information about design of technical systems: No further data; see item 7.

· 8.1 Control parameters· Components with limit values that require monitoring at the workplace:The product does not contain any relevant quantities of materials with critical values that have to be monitored at theworkplace.

· Additional information: The lists that were valid during the creation were used as basis.(Contd. on page 3)

IE

Page 4: Product code Description€¦ · Printing date 26.06.2015 Revision: 22.06.2015 40.1.5 SECTION 1: Identification of the substance/mixture and of the company/undertaking · 1.1 Product

Page 3/6

Safety data sheetaccording to 1907/2006/EC, Article 31

Printing date 26.06.2015 Revision: 22.06.2015

Trade name: NGM Agar Lite

(Contd. of page 2)

40.1.5

· 8.2 Exposure controls· Personal protective equipment· General protective and hygienic measuresThe usual precautionary measures should be adhered to when handling chemicals.

· Protection of hands: Protective gloves.· Material of gloves Synthetic gloves· Penetration time of glove materialThe exact break trough time has to be found out by the manufacturer of the protective gloves and has to be observed.

· Eye protection: Safety glasses

SECTION 9: Physical and chemical properties

· 9.1 Information on basic physical and chemical properties· General Information· Appearance:

Form: Solid.Colour: Light yellow

· Odour: Odourless· Odour threshold: Not determined.

· pH-value: Not applicable.

· Change in conditionMelting point/Melting range: undeterminedBoiling point/Boiling range: undetermined

· Flash point: Not applicable

· Flammability (solid, gaseous) Not determined.

· Ignition temperature:

Decomposition temperature: Not determined.

· Self igniting: Product is not selfigniting.

· Danger of explosion: Product does not present an explosion hazard.

· Explosion limits:Lower: Not determined.Upper: Not determined.

· Vapour pressure: Not applicable.

· Density: Not determined· Relative density Not determined.· Vapour density Not applicable.· Evaporation rate Not applicable.

· Solubility in / Miscibility withWater: Fully miscible

· Partition coefficient (n-octanol/water): Not determined.

· Viscosity:dynamic: Not applicable.

(Contd. on page 4) IE

Page 5: Product code Description€¦ · Printing date 26.06.2015 Revision: 22.06.2015 40.1.5 SECTION 1: Identification of the substance/mixture and of the company/undertaking · 1.1 Product

Page 4/6

Safety data sheetaccording to 1907/2006/EC, Article 31

Printing date 26.06.2015 Revision: 22.06.2015

Trade name: NGM Agar Lite

(Contd. of page 3)

40.1.5

kinematic: Not applicable.

· Solvent content:Organic solvents: 0,0 %

Solids content: 100,0 %· 9.2 Other information No further relevant information available.

SECTION 10: Stability and reactivity

· 10.1 Reactivity· 10.2 Chemical stability· Thermal decomposition / conditions to be avoided: No decomposition if used according to specifications.· 10.3 Possibility of hazardous reactions No dangerous reactions known· 10.4 Conditions to avoid No further relevant information available.· 10.5 Incompatible materials: No further relevant information available.· 10.6 Hazardous decomposition products: No dangerous decomposition products known

* SECTION 11: Toxicological information

· 11.1 Information on toxicological effects· Acute toxicity:· LD/LC50 values that are relevant for classification:

9002-18-0 AgarOral LD50 11000 mg/kg (rat)

· Primary irritant effect:· on the skin: No irritant effect.· on the eye: Irritating effect.· Sensitisation: No sensitising effects known.· Additional toxicological information:The product is not subject to classification according to the calculation method of the General EU Classification Guidelinesfor Preparations as issued in the latest version:When used and handled according to specifications, the product does not have any harmful effects according to ourexperience and the information provided to us.

SECTION 12: Ecological information

· 12.1 Toxicity· Aquatic toxicity: No further relevant information available.· 12.2 Persistence and degradability No further relevant information available.· 12.3 Bioaccumulative potential No further relevant information available.· 12.4 Mobility in soil No further relevant information available.· Additional ecological information:· General notes:Water hazard class 1 (German Regulation) (Self-assessment): slightly hazardous for water.Do not allow undiluted product or large quantities of it to reach ground water, water course or sewage system.

(Contd. on page 5) IE

Page 6: Product code Description€¦ · Printing date 26.06.2015 Revision: 22.06.2015 40.1.5 SECTION 1: Identification of the substance/mixture and of the company/undertaking · 1.1 Product

Page 5/6

Safety data sheetaccording to 1907/2006/EC, Article 31

Printing date 26.06.2015 Revision: 22.06.2015

Trade name: NGM Agar Lite

(Contd. of page 4)

40.1.5

· 12.5 Results of PBT and vPvB assessment· PBT: Not applicable.· vPvB: Not applicable.· 12.6 Other adverse effects No further relevant information available.

SECTION 13: Disposal considerations

· 13.1 Waste treatment methods· RecommendationMust not be disposed of together with household garbage. Do not allow product to reach sewage system.

· Uncleaned packagings:· Recommendation: Disposal must be made according to official regulations.· Recommended cleansing agent: Water, if necessary with cleansing agents.

SECTION 14: Transport information

· 14.1 UN-Number· ADR, ADN, IMDG, IATA Void

· 14.2 UN proper shipping name· ADR, ADN, IMDG, IATA Void

· 14.3 Transport hazard class(es)

· ADR, ADN, IMDG, IATA· Class Void

· 14.4 Packing group· ADR, IMDG, IATA Void

· 14.5 Environmental hazards:· Marine pollutant: No

· 14.6 Special precautions for user Not applicable.

· 14.7 Transport in bulk according to Annex II ofMARPOL73/78 and the IBC Code Not applicable.

· UN "Model Regulation": -

* SECTION 15: Regulatory information

· 15.1 Safety, health and environmental regulations/legislation specific for the substance or mixture

· National regulations

· Water hazard class: Water hazard class 1 (Self-assessment): slightly hazardous for water.· 15.2 Chemical safety assessment: A Chemical Safety Assessment has not been carried out.

IE

(Contd. on page 6)

Page 7: Product code Description€¦ · Printing date 26.06.2015 Revision: 22.06.2015 40.1.5 SECTION 1: Identification of the substance/mixture and of the company/undertaking · 1.1 Product

Page 6/6

Safety data sheetaccording to 1907/2006/EC, Article 31

Printing date 26.06.2015 Revision: 22.06.2015

Trade name: NGM Agar Lite

(Contd. of page 5)

40.1.5

SECTION 16: Other informationThis information is based on our present knowledge. However, this shall not constitute a guarantee for any specific productfeatures and shall not establish a legally valid contractual relationship.

· Relevant phrasesH315 Causes skin irritation.H319 Causes serious eye irritation.

· Department issuing SDS: Environmental Health and Safety.· Contact:Life Science Group, Environmental Health and Safety, 2000 Alfred Nobel Drive, Hercules, California, 94547: 1(510) 741-1000

Diagnostic Group, Environmental Health and Safety, 4000 Alfred Nobel Drive, Hercules, California, 94547: 1(510) 724-7000· Abbreviations and acronyms:

ADR: Accord européen sur le transport des marchandises dangereuses par Route (European Agreement concerning the International Carriage ofDangerous Goods by Road)IMDG: International Maritime Code for Dangerous GoodsIATA: International Air Transport AssociationGHS: Globally Harmonised System of Classification and Labelling of ChemicalsEINECS: European Inventory of Existing Commercial Chemical SubstancesELINCS: European List of Notified Chemical SubstancesCAS: Chemical Abstracts Service (division of the American Chemical Society)LC50: Lethal concentration, 50 percentLD50: Lethal dose, 50 percentSkin Irrit. 2: Skin corrosion/irritation, Hazard Category 2Eye Irrit. 2: Serious eye damage/eye irritation, Hazard Category 2

· * Data compared to the previous version altered. IE

Page 8: Product code Description€¦ · Printing date 26.06.2015 Revision: 22.06.2015 40.1.5 SECTION 1: Identification of the substance/mixture and of the company/undertaking · 1.1 Product

Page 1/6

Safety data sheetaccording to 1907/2006/EC, Article 31

Printing date 26.06.2015 Revision: 22.06.2015

40.1.5

SECTION 1: Identification of the substance/mixture and of the company/undertaking

· 1.1 Product identifier

· Trade name: Agar

· Article number: 10030934, 10039857· CAS Number:9002-18-0

· EC number:232-658-1

· 1.2 Relevant identified uses of the substance or mixture and uses advised againstNo further relevant information available.

· Application of the substance / the mixture Laboratory chemicals

· 1.3 Details of the supplier of the safety data sheet· Manufacturer/Supplier:Bio-Rad Laboratories S.r.l.Via Cellini, 18A20090 Segrate-MilanoItalyphone: 39-02-216091 fax: [email protected] / [email protected]

· Information department:Technical services, customer [email protected] / [email protected]

· 1.4 Emergency telephone number: 02-216091 - active during office hours for technical information

* SECTION 2: Hazards identification

· 2.1 Classification of the substance or mixture· Classification according to Regulation (EC) No 1272/2008The substance is not classified according to the CLP regulation.

· 2.2 Label elements· Labelling according to Regulation (EC) No 1272/2008 Void· Hazard pictograms Void· Signal word Void· Hazard statements Void· 2.3 Other hazards· Results of PBT and vPvB assessment· PBT: Not applicable.· vPvB: Not applicable.

SECTION 3: Composition/information on ingredients

· 3.1 Chemical characterisation: Substances· CAS No. Description:9002-18-0 Agar

· Identification number(s):· EC number: 232-658-1· Additonal information: For the wording of the listed risk phrases refer to section 16.

IE

(Contd. on page 2)

Page 9: Product code Description€¦ · Printing date 26.06.2015 Revision: 22.06.2015 40.1.5 SECTION 1: Identification of the substance/mixture and of the company/undertaking · 1.1 Product

Page 2/6

Safety data sheetaccording to 1907/2006/EC, Article 31

Printing date 26.06.2015 Revision: 22.06.2015

Trade name: Agar

(Contd. of page 1)

40.1.5

SECTION 4: First aid measures

· 4.1 Description of first aid measures· After inhalation Supply fresh air; consult doctor in case of complaints.· After eye contact Rinse opened eye for several minutes under running water.· After swallowing Induce vomiting and call for medical help.· 4.2 Most important symptoms and effects, both acute and delayed No further relevant information available.· 4.3 Indication of any immediate medical attention and special treatment neededNo further relevant information available.

SECTION 5: Firefighting measures

· 5.1 Extinguishing media· Suitable extinguishing agentsCO2, extinguishing powder or water spray. Fight larger fires with water spray or alcohol resistant foam.

· 5.2 Special hazards arising from the substance or mixture No further relevant information available.· 5.3 Advice for firefighters· Protective equipment: No special measures required.

* SECTION 6: Accidental release measures

· 6.1 Personal precautions, protective equipment and emergency procedures Wear protective clothing.· 6.2 Environmental precautions: No special measures required.· 6.3 Methods and material for containment and cleaning up: Pick up mechanically.· 6.4 Reference to other sectionsNo dangerous substances are released.See Section 7 for information on safe handlingSee Section 8 for information on personal protection equipment.See Section 13 for disposal information.

SECTION 7: Handling and storage

· 7.1 Precautions for safe handling No special precautions are necessary if used correctly.· Information about protection against explosions and fires: No special measures required.

· 7.2 Conditions for safe storage, including any incompatibilities· Storage· Requirements to be met by storerooms and receptacles: According to product specification· Information about storage in one common storage facility: Not required.· Further information about storage conditions: None.· 7.3 Specific end use(s) No further relevant information available.

SECTION 8: Exposure controls/personal protection

· Additional information about design of technical systems: No further data; see item 7.

· 8.1 Control parameters· Components with limit values that require monitoring at the workplace: Not required.

(Contd. on page 3) IE

Page 10: Product code Description€¦ · Printing date 26.06.2015 Revision: 22.06.2015 40.1.5 SECTION 1: Identification of the substance/mixture and of the company/undertaking · 1.1 Product

Page 3/6

Safety data sheetaccording to 1907/2006/EC, Article 31

Printing date 26.06.2015 Revision: 22.06.2015

Trade name: Agar

(Contd. of page 2)

40.1.5

· Additional information: The lists that were valid during the creation were used as basis.

· 8.2 Exposure controls· Personal protective equipment· General protective and hygienic measuresThe usual precautionary measures should be adhered to when handling chemicals.

· Protection of hands: Protective gloves.· Material of gloves Synthetic gloves· Penetration time of glove materialThe exact break trough time has to be found out by the manufacturer of the protective gloves and has to be observed.

· Eye protection: Safety glasses

SECTION 9: Physical and chemical properties

· 9.1 Information on basic physical and chemical properties· General Information· Appearance:

Form: Solid.Colour: Light yellow

· Odour: Odourless· Odour threshold: Not determined.

· pH-value: Not applicable.

· Change in conditionMelting point/Melting range: undeterminedBoiling point/Boiling range: undetermined

· Flash point: Not applicable

· Flammability (solid, gaseous) Product is not flammable.

· Ignition temperature:

Decomposition temperature: Not determined.

· Self igniting: Not determined.

· Danger of explosion: Product does not present an explosion hazard.

· Explosion limits:Lower: Not determined.Upper: Not determined.

· Vapour pressure: Not applicable.

· Density at 20 °C: 0,55 g/cm³

· Bulk density at 20 °C: 550 kg/m³· Relative density Not determined.· Vapour density Not applicable.· Evaporation rate Not applicable.

· Solubility in / Miscibility withWater at 60 °C: 20 g/l

Fully miscible

(Contd. on page 4) IE

Page 11: Product code Description€¦ · Printing date 26.06.2015 Revision: 22.06.2015 40.1.5 SECTION 1: Identification of the substance/mixture and of the company/undertaking · 1.1 Product

Page 4/6

Safety data sheetaccording to 1907/2006/EC, Article 31

Printing date 26.06.2015 Revision: 22.06.2015

Trade name: Agar

(Contd. of page 3)

40.1.5

· Partition coefficient (n-octanol/water): Not determined.

· Viscosity:dynamic: Not applicable.kinematic: Not applicable.Organic solvents: 0,0 %

Solids content: 100,0 %· 9.2 Other information No further relevant information available.

SECTION 10: Stability and reactivity

· 10.1 Reactivity· 10.2 Chemical stability· Thermal decomposition / conditions to be avoided: No decomposition if used according to specifications.· 10.3 Possibility of hazardous reactions No dangerous reactions known· 10.4 Conditions to avoid No further relevant information available.· 10.5 Incompatible materials: No further relevant information available.· 10.6 Hazardous decomposition products: No dangerous decomposition products known

* SECTION 11: Toxicological information

· 11.1 Information on toxicological effects· Acute toxicity:· LD/LC50 values that are relevant for classification:

9002-18-0 AgarOral LD50 11000 mg/kg (rat)

· Primary irritant effect:· on the skin: No irritant effect.· on the eye: Irritating effect.· Sensitisation: No sensitising effects known.· Additional toxicological information:When used and handled according to specifications, the product does not have any harmful effects according to ourexperience and the information provided to us.The substance is not subject to classification according to the latest version of the EU lists.

SECTION 12: Ecological information

· 12.1 Toxicity· Aquatic toxicity: No further relevant information available.· 12.2 Persistence and degradability No further relevant information available.· 12.3 Bioaccumulative potential No further relevant information available.· 12.4 Mobility in soil No further relevant information available.· Additional ecological information:· General notes: Generally not hazardous for water.· 12.5 Results of PBT and vPvB assessment· PBT: Not applicable.

(Contd. on page 5) IE

Page 12: Product code Description€¦ · Printing date 26.06.2015 Revision: 22.06.2015 40.1.5 SECTION 1: Identification of the substance/mixture and of the company/undertaking · 1.1 Product

Page 5/6

Safety data sheetaccording to 1907/2006/EC, Article 31

Printing date 26.06.2015 Revision: 22.06.2015

Trade name: Agar

(Contd. of page 4)

40.1.5

· vPvB: Not applicable.· 12.6 Other adverse effects No further relevant information available.

SECTION 13: Disposal considerations

· 13.1 Waste treatment methods· RecommendationMust not be disposed of together with household garbage. Do not allow product to reach sewage system.

· Uncleaned packagings:· Recommendation: Disposal must be made according to official regulations.

SECTION 14: Transport information

· 14.1 UN-Number· ADR, ADN, IMDG, IATA Void

· 14.2 UN proper shipping name· ADR, ADN, IMDG, IATA Void

· 14.3 Transport hazard class(es)

· ADR, ADN, IMDG, IATA· Class Void

· 14.4 Packing group· ADR, IMDG, IATA Void

· 14.5 Environmental hazards:· Marine pollutant: No

· 14.6 Special precautions for user Not applicable.

· 14.7 Transport in bulk according to Annex II ofMARPOL73/78 and the IBC Code Not applicable.

· UN "Model Regulation": -

* SECTION 15: Regulatory information

· 15.1 Safety, health and environmental regulations/legislation specific for the substance or mixture

· National regulations

· Water hazard class: Generally not hazardous for water.· 15.2 Chemical safety assessment: A Chemical Safety Assessment has not been carried out.

SECTION 16: Other informationThis information is based on our present knowledge. However, this shall not constitute a guarantee for any specific productfeatures and shall not establish a legally valid contractual relationship.

· Department issuing SDS: Environmental Health and Safety.(Contd. on page 6)

IE

Page 13: Product code Description€¦ · Printing date 26.06.2015 Revision: 22.06.2015 40.1.5 SECTION 1: Identification of the substance/mixture and of the company/undertaking · 1.1 Product

Page 6/6

Safety data sheetaccording to 1907/2006/EC, Article 31

Printing date 26.06.2015 Revision: 22.06.2015

Trade name: Agar

(Contd. of page 5)

40.1.5

· Contact:Life Science Group, Environmental Health and Safety, 2000 Alfred Nobel Drive, Hercules, California, 94547: 1(510) 741-1000

Diagnostic Group, Environmental Health and Safety, 4000 Alfred Nobel Drive, Hercules, California, 94547: 1(510) 724-7000· Abbreviations and acronyms:

ADR: Accord européen sur le transport des marchandises dangereuses par Route (European Agreement concerning the International Carriage ofDangerous Goods by Road)IMDG: International Maritime Code for Dangerous GoodsIATA: International Air Transport AssociationGHS: Globally Harmonised System of Classification and Labelling of ChemicalsEINECS: European Inventory of Existing Commercial Chemical SubstancesCAS: Chemical Abstracts Service (division of the American Chemical Society)LC50: Lethal concentration, 50 percentLD50: Lethal dose, 50 percent

· * Data compared to the previous version altered. IE

Page 14: Product code Description€¦ · Printing date 26.06.2015 Revision: 22.06.2015 40.1.5 SECTION 1: Identification of the substance/mixture and of the company/undertaking · 1.1 Product

Page 1/6

Safety data sheetaccording to 1907/2006/EC, Article 31

Printing date 26.06.2015 Revision: 22.06.2015

40.1.5

SECTION 1: Identification of the substance/mixture and of the company/undertaking

· 1.1 Product identifier

· Trade name: 2.5 M NaCl Wash Buffer

· Article number: 10030936· 1.2 Relevant identified uses of the substance or mixture and uses advised againstNo further relevant information available.

· Application of the substance / the mixture Laboratory chemicals

· 1.3 Details of the supplier of the safety data sheet· Manufacturer/Supplier:Bio-Rad Laboratories S.r.l.Via Cellini, 18A20090 Segrate-MilanoItalyphone: 39-02-216091 fax: [email protected] / [email protected]

· Information department:Technical services, customer [email protected] / [email protected]

· 1.4 Emergency telephone number: 02-216091 - active during office hours for technical information

* SECTION 2: Hazards identification

· 2.1 Classification of the substance or mixture· Classification according to Regulation (EC) No 1272/2008Skin Irrit. 2 H315 Causes skin irritation.Eye Irrit. 2 H319 Causes serious eye irritation.

· 2.2 Label elements· Labelling according to Regulation (EC) No 1272/2008The product is classified and labelled according to the CLP regulation.

· Hazard pictograms

GHS07

· Signal word Warning· Hazard statementsH315 Causes skin irritation.H319 Causes serious eye irritation.

· Precautionary statementsP280 Wear protective gloves/protective clothing/eye protection/face protection.P264 Wash thoroughly after handling.P305+P351+P338 IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy

to do. Continue rinsing.P362 Take off contaminated clothing and wash before reuse.P332+P313 If skin irritation occurs: Get medical advice/attention.P337+P313 If eye irritation persists: Get medical advice/attention.

(Contd. on page 2) IE

Page 15: Product code Description€¦ · Printing date 26.06.2015 Revision: 22.06.2015 40.1.5 SECTION 1: Identification of the substance/mixture and of the company/undertaking · 1.1 Product

Page 2/6

Safety data sheetaccording to 1907/2006/EC, Article 31

Printing date 26.06.2015 Revision: 22.06.2015

Trade name: 2.5 M NaCl Wash Buffer

(Contd. of page 1)

40.1.5

· 2.3 Other hazards· Results of PBT and vPvB assessment· PBT: Not applicable.· vPvB: Not applicable.

* SECTION 3: Composition/information on ingredients

· 3.2 Chemical characterisation: Mixtures· Description: Mixture of the substances listed below with nonhazardous additions.· Dangerous components:CAS: 7647-14-5EINECS: 231-598-3

sodium chloride Skin Irrit. 2, H315; Eye Irrit. 2, H319 10-20%

· Additional information For the wording of the listed risk phrases refer to section 16.

SECTION 4: First aid measures

· 4.1 Description of first aid measures· After inhalation Supply fresh air; consult doctor in case of complaints.· After eye contact Rinse opened eye for several minutes under running water.· After swallowing Induce vomiting and call for medical help.· 4.2 Most important symptoms and effects, both acute and delayed No further relevant information available.· 4.3 Indication of any immediate medical attention and special treatment neededNo further relevant information available.

SECTION 5: Firefighting measures

· 5.1 Extinguishing media· Suitable extinguishing agentsCO2, extinguishing powder or water spray. Fight larger fires with water spray or alcohol resistant foam.

· 5.2 Special hazards arising from the substance or mixture No further relevant information available.· 5.3 Advice for firefighters· Protective equipment: No special measures required.

* SECTION 6: Accidental release measures

· 6.1 Personal precautions, protective equipment and emergency procedures Wear protective clothing.· 6.2 Environmental precautions:Dilute with plenty of water.Do not allow to enter sewers/ surface or ground water.

· 6.3 Methods and material for containment and cleaning up:Absorb with liquid-binding material (sand, diatomite, acid binders, universal binders, sawdust).

· 6.4 Reference to other sectionsNo dangerous substances are released.See Section 7 for information on safe handlingSee Section 8 for information on personal protection equipment.

(Contd. on page 3) IE

Page 16: Product code Description€¦ · Printing date 26.06.2015 Revision: 22.06.2015 40.1.5 SECTION 1: Identification of the substance/mixture and of the company/undertaking · 1.1 Product

Page 3/6

Safety data sheetaccording to 1907/2006/EC, Article 31

Printing date 26.06.2015 Revision: 22.06.2015

Trade name: 2.5 M NaCl Wash Buffer

(Contd. of page 2)

40.1.5

See Section 13 for disposal information.

SECTION 7: Handling and storage

· 7.1 Precautions for safe handling No special precautions are necessary if used correctly.· Information about protection against explosions and fires: No special measures required.

· 7.2 Conditions for safe storage, including any incompatibilities· Storage· Requirements to be met by storerooms and receptacles: According to product specification· Information about storage in one common storage facility: Not required.· Further information about storage conditions: None.· 7.3 Specific end use(s) No further relevant information available.

* SECTION 8: Exposure controls/personal protection

· Additional information about design of technical systems: No further data; see item 7.

· 8.1 Control parameters· Components with limit values that require monitoring at the workplace:The product does not contain any relevant quantities of materials with critical values that have to be monitored at theworkplace.

· Additional information: The lists that were valid during the creation were used as basis.

· 8.2 Exposure controls· Personal protective equipment· General protective and hygienic measures Wash hands before breaks and at the end of work.· Breathing equipment: Not required.· Protection of hands: Protective gloves.· Material of gloves Synthetic gloves· Penetration time of glove materialThe exact break trough time has to be found out by the manufacturer of the protective gloves and has to be observed.

· Eye protection: Safety glasses

SECTION 9: Physical and chemical properties

· 9.1 Information on basic physical and chemical properties· General Information· Appearance:

Form: LiquidColour: Colourless

· Odour: Odourless· Odour threshold: Not determined.

· pH-value at 20 °C: 5,5

· Change in conditionMelting point/Melting range: undeterminedBoiling point/Boiling range: undetermined

(Contd. on page 4) IE

Page 17: Product code Description€¦ · Printing date 26.06.2015 Revision: 22.06.2015 40.1.5 SECTION 1: Identification of the substance/mixture and of the company/undertaking · 1.1 Product

Page 4/6

Safety data sheetaccording to 1907/2006/EC, Article 31

Printing date 26.06.2015 Revision: 22.06.2015

Trade name: 2.5 M NaCl Wash Buffer

(Contd. of page 3)

40.1.5

· Flash point: Not applicable

· Flammability (solid, gaseous) Not applicable.

· Ignition temperature:

Decomposition temperature: Not determined.

· Self igniting: Product is not selfigniting.

· Danger of explosion: Product does not present an explosion hazard.

· Explosion limits:Lower: Not determined.Upper: Not determined.

· Vapour pressure at 20 °C: 23 hPa

· Density: Not determined· Relative density Not determined.· Vapour density Not determined.· Evaporation rate Not determined.

· Solubility in / Miscibility withWater: Fully miscible

· Partition coefficient (n-octanol/water): Not determined.

· Viscosity:dynamic: Not determined.kinematic: Not determined.

· Solvent content:Organic solvents: 0,0 %Water: 87,1 %

Solids content: 12,9 %· 9.2 Other information No further relevant information available.

SECTION 10: Stability and reactivity

· 10.1 Reactivity· 10.2 Chemical stability· Thermal decomposition / conditions to be avoided: No decomposition if used according to specifications.· 10.3 Possibility of hazardous reactions No dangerous reactions known· 10.4 Conditions to avoid No further relevant information available.· 10.5 Incompatible materials: No further relevant information available.· 10.6 Hazardous decomposition products: No dangerous decomposition products known

IE

(Contd. on page 5)

Page 18: Product code Description€¦ · Printing date 26.06.2015 Revision: 22.06.2015 40.1.5 SECTION 1: Identification of the substance/mixture and of the company/undertaking · 1.1 Product

Page 5/6

Safety data sheetaccording to 1907/2006/EC, Article 31

Printing date 26.06.2015 Revision: 22.06.2015

Trade name: 2.5 M NaCl Wash Buffer

(Contd. of page 4)

40.1.5

* SECTION 11: Toxicological information

· 11.1 Information on toxicological effects· Acute toxicity:· Primary irritant effect:· on the skin: Irritant to skin and mucous membranes.· on the eye: Irritating effect.· Sensitisation: No sensitising effects known.

SECTION 12: Ecological information

· 12.1 Toxicity· Aquatic toxicity: No further relevant information available.· 12.2 Persistence and degradability No further relevant information available.· 12.3 Bioaccumulative potential No further relevant information available.· 12.4 Mobility in soil No further relevant information available.· Additional ecological information:· General notes:Water hazard class 1 (German Regulation) (Self-assessment): slightly hazardous for water.Do not allow undiluted product or large quantities of it to reach ground water, water course or sewage system.

· 12.5 Results of PBT and vPvB assessment· PBT: Not applicable.· vPvB: Not applicable.· 12.6 Other adverse effects No further relevant information available.

SECTION 13: Disposal considerations

· 13.1 Waste treatment methods· RecommendationMust not be disposed of together with household garbage. Do not allow product to reach sewage system.

· Uncleaned packagings:· Recommendation: Disposal must be made according to official regulations.· Recommended cleansing agent: Water, if necessary with cleansing agents.

SECTION 14: Transport information

· 14.1 UN-Number· ADR, ADN, IMDG, IATA Void

· 14.2 UN proper shipping name· ADR, ADN, IMDG, IATA Void

· 14.3 Transport hazard class(es)

· ADR, ADN, IMDG, IATA· Class Void

(Contd. on page 6) IE

Page 19: Product code Description€¦ · Printing date 26.06.2015 Revision: 22.06.2015 40.1.5 SECTION 1: Identification of the substance/mixture and of the company/undertaking · 1.1 Product

Page 6/6

Safety data sheetaccording to 1907/2006/EC, Article 31

Printing date 26.06.2015 Revision: 22.06.2015

Trade name: 2.5 M NaCl Wash Buffer

(Contd. of page 5)

40.1.5

· 14.4 Packing group· ADR, IMDG, IATA Void

· 14.5 Environmental hazards:· Marine pollutant: No

· 14.6 Special precautions for user Not applicable.

· 14.7 Transport in bulk according to Annex II ofMARPOL73/78 and the IBC Code Not applicable.

· UN "Model Regulation": -

* SECTION 15: Regulatory information

· 15.1 Safety, health and environmental regulations/legislation specific for the substance or mixture

· National regulations

· Water hazard class: Water hazard class 1 (Self-assessment): slightly hazardous for water.· 15.2 Chemical safety assessment: A Chemical Safety Assessment has not been carried out.

SECTION 16: Other informationThis information is based on our present knowledge. However, this shall not constitute a guarantee for any specific productfeatures and shall not establish a legally valid contractual relationship.

· Relevant phrasesH315 Causes skin irritation.H319 Causes serious eye irritation.

· Department issuing SDS: Environmental Health and Safety.· Contact:Life Science Group, Environmental Health and Safety, 2000 Alfred Nobel Drive, Hercules, California, 94547: 1(510) 741-1000

Diagnostic Group, Environmental Health and Safety, 4000 Alfred Nobel Drive, Hercules, California, 94547: 1(510) 724-7000· Abbreviations and acronyms:

ADR: Accord européen sur le transport des marchandises dangereuses par Route (European Agreement concerning the International Carriage ofDangerous Goods by Road)IMDG: International Maritime Code for Dangerous GoodsIATA: International Air Transport AssociationGHS: Globally Harmonised System of Classification and Labelling of ChemicalsEINECS: European Inventory of Existing Commercial Chemical SubstancesELINCS: European List of Notified Chemical SubstancesCAS: Chemical Abstracts Service (division of the American Chemical Society)Skin Irrit. 2: Skin corrosion/irritation, Hazard Category 2Eye Irrit. 2: Serious eye damage/eye irritation, Hazard Category 2

· * Data compared to the previous version altered. IE

Page 20: Product code Description€¦ · Printing date 26.06.2015 Revision: 22.06.2015 40.1.5 SECTION 1: Identification of the substance/mixture and of the company/undertaking · 1.1 Product

Page 1/6

Safety data sheetaccording to 1907/2006/EC, Article 31

Printing date 26.06.2015 Revision: 22.06.2015

40.1.5

SECTION 1: Identification of the substance/mixture and of the company/undertaking

· 1.1 Product identifier

· Trade name: C. elegans 10X Wash Buffer

· Article number: 10030937· 1.2 Relevant identified uses of the substance or mixture and uses advised againstNo further relevant information available.

· Application of the substance / the mixture Laboratory chemicals

· 1.3 Details of the supplier of the safety data sheet· Manufacturer/Supplier:Bio-Rad Laboratories S.r.l.Via Cellini, 18A20090 Segrate-MilanoItalyphone: 39-02-216091 fax: [email protected] / [email protected]

· Information department:Technical services, customer [email protected] / [email protected]

· 1.4 Emergency telephone number: 02-216091 - active during office hours for technical information

* SECTION 2: Hazards identification

· 2.1 Classification of the substance or mixture· Classification according to Regulation (EC) No 1272/2008 The product is not classified according to the CLP regulation.

· 2.2 Label elements· Labelling according to Regulation (EC) No 1272/2008 Void· Hazard pictograms Void· Signal word Void· Hazard statements Void· 2.3 Other hazards· Results of PBT and vPvB assessment· PBT: Not applicable.· vPvB: Not applicable.

SECTION 3: Composition/information on ingredients

· 3.2 Chemical characterisation: Mixtures· Description: Mixture of the substances listed below with nonhazardous additions.

· Dangerous components: Void· Additional information For the wording of the listed risk phrases refer to section 16.

SECTION 4: First aid measures

· 4.1 Description of first aid measures· After inhalation Supply fresh air; consult doctor in case of complaints.· After eye contact Rinse opened eye for several minutes under running water.· After swallowing Induce vomiting and call for medical help.· 4.2 Most important symptoms and effects, both acute and delayed No further relevant information available.

(Contd. on page 2) IE

Page 21: Product code Description€¦ · Printing date 26.06.2015 Revision: 22.06.2015 40.1.5 SECTION 1: Identification of the substance/mixture and of the company/undertaking · 1.1 Product

Page 2/6

Safety data sheetaccording to 1907/2006/EC, Article 31

Printing date 26.06.2015 Revision: 22.06.2015

Trade name: C. elegans 10X Wash Buffer

(Contd. of page 1)

40.1.5

· 4.3 Indication of any immediate medical attention and special treatment neededNo further relevant information available.

SECTION 5: Firefighting measures

· 5.1 Extinguishing media· Suitable extinguishing agentsCO2, extinguishing powder or water spray. Fight larger fires with water spray or alcohol resistant foam.

· 5.2 Special hazards arising from the substance or mixture No further relevant information available.· 5.3 Advice for firefighters· Protective equipment: No special measures required.

SECTION 6: Accidental release measures

· 6.1 Personal precautions, protective equipment and emergency procedures Wear protective clothing.· 6.2 Environmental precautions: Dilute with plenty of water.· 6.3 Methods and material for containment and cleaning up:Absorb with liquid-binding material (sand, diatomite, acid binders, universal binders, sawdust).

· 6.4 Reference to other sectionsNo dangerous substances are released.See Section 7 for information on safe handlingSee Section 8 for information on personal protection equipment.See Section 13 for disposal information.

SECTION 7: Handling and storage

· 7.1 Precautions for safe handling No special precautions are necessary if used correctly.· Information about protection against explosions and fires: No special measures required.

· 7.2 Conditions for safe storage, including any incompatibilities· Storage· Requirements to be met by storerooms and receptacles: According to product specification· Information about storage in one common storage facility: Not required.· Further information about storage conditions: None.· 7.3 Specific end use(s) No further relevant information available.

SECTION 8: Exposure controls/personal protection

· Additional information about design of technical systems: No further data; see item 7.

· 8.1 Control parameters· Components with limit values that require monitoring at the workplace:The product does not contain any relevant quantities of materials with critical values that have to be monitored at theworkplace.

· Additional information: The lists that were valid during the creation were used as basis.(Contd. on page 3)

IE

Page 22: Product code Description€¦ · Printing date 26.06.2015 Revision: 22.06.2015 40.1.5 SECTION 1: Identification of the substance/mixture and of the company/undertaking · 1.1 Product

Page 3/6

Safety data sheetaccording to 1907/2006/EC, Article 31

Printing date 26.06.2015 Revision: 22.06.2015

Trade name: C. elegans 10X Wash Buffer

(Contd. of page 2)

40.1.5

· 8.2 Exposure controls· Personal protective equipment· General protective and hygienic measuresThe usual precautionary measures should be adhered to when handling chemicals.

· Breathing equipment: Not required.· Protection of hands: Protective gloves.· Material of gloves Synthetic gloves· Penetration time of glove materialThe exact break trough time has to be found out by the manufacturer of the protective gloves and has to be observed.

· Eye protection: Safety glasses

SECTION 9: Physical and chemical properties

· 9.1 Information on basic physical and chemical properties· General Information· Appearance:

Form: LiquidColour: Colourless

· Odour: Odourless· Odour threshold: Not determined.

· pH-value at 20 °C: 6

· Change in conditionMelting point/Melting range: undeterminedBoiling point/Boiling range: undetermined

· Flash point: Not applicable

· Flammability (solid, gaseous) Not applicable.

· Ignition temperature:

Decomposition temperature: Not determined.

· Self igniting: Product is not selfigniting.

· Danger of explosion: Product does not present an explosion hazard.

· Explosion limits:Lower: Not determined.Upper: Not determined.

· Vapour pressure at 20 °C: 23 hPa

· Density: Not determined· Relative density Not determined.· Vapour density Not determined.· Evaporation rate Not determined.

· Solubility in / Miscibility withWater: Fully miscible

· Partition coefficient (n-octanol/water): Not determined.

(Contd. on page 4) IE

Page 23: Product code Description€¦ · Printing date 26.06.2015 Revision: 22.06.2015 40.1.5 SECTION 1: Identification of the substance/mixture and of the company/undertaking · 1.1 Product

Page 4/6

Safety data sheetaccording to 1907/2006/EC, Article 31

Printing date 26.06.2015 Revision: 22.06.2015

Trade name: C. elegans 10X Wash Buffer

(Contd. of page 3)

40.1.5

· Viscosity:dynamic: Not determined.kinematic: Not determined.

· Solvent content:Organic solvents: 0,0 %Water: 98,3 %

Solids content: 1,7 %· 9.2 Other information No further relevant information available.

SECTION 10: Stability and reactivity

· 10.1 Reactivity· 10.2 Chemical stability· Thermal decomposition / conditions to be avoided: No decomposition if used according to specifications.· 10.3 Possibility of hazardous reactions No dangerous reactions known· 10.4 Conditions to avoid No further relevant information available.· 10.5 Incompatible materials: No further relevant information available.· 10.6 Hazardous decomposition products: No dangerous decomposition products known

* SECTION 11: Toxicological information

· 11.1 Information on toxicological effects· Acute toxicity:· Primary irritant effect:· on the skin: No irritant effect.· on the eye: Irritating effect.· Sensitisation: No sensitising effects known.· Additional toxicological information:The product is not subject to classification according to the calculation method of the General EU Classification Guidelinesfor Preparations as issued in the latest version:When used and handled according to specifications, the product does not have any harmful effects according to ourexperience and the information provided to us.

SECTION 12: Ecological information

· 12.1 Toxicity· Aquatic toxicity: No further relevant information available.· 12.2 Persistence and degradability No further relevant information available.· 12.3 Bioaccumulative potential No further relevant information available.· 12.4 Mobility in soil No further relevant information available.· Additional ecological information:· General notes: Generally not hazardous for water.· 12.5 Results of PBT and vPvB assessment· PBT: Not applicable.· vPvB: Not applicable.

(Contd. on page 5) IE

Page 24: Product code Description€¦ · Printing date 26.06.2015 Revision: 22.06.2015 40.1.5 SECTION 1: Identification of the substance/mixture and of the company/undertaking · 1.1 Product

Page 5/6

Safety data sheetaccording to 1907/2006/EC, Article 31

Printing date 26.06.2015 Revision: 22.06.2015

Trade name: C. elegans 10X Wash Buffer

(Contd. of page 4)

40.1.5

· 12.6 Other adverse effects No further relevant information available.

SECTION 13: Disposal considerations

· 13.1 Waste treatment methods· RecommendationMust not be disposed of together with household garbage. Do not allow product to reach sewage system.

· Uncleaned packagings:· Recommendation: Disposal must be made according to official regulations.· Recommended cleansing agent: Water, if necessary with cleansing agents.

SECTION 14: Transport information

· 14.1 UN-Number· ADR, ADN, IMDG, IATA Void

· 14.2 UN proper shipping name· ADR, ADN, IMDG, IATA Void

· 14.3 Transport hazard class(es)

· ADR, ADN, IMDG, IATA· Class Void

· 14.4 Packing group· ADR, IMDG, IATA Void

· 14.5 Environmental hazards:· Marine pollutant: No

· 14.6 Special precautions for user Not applicable.

· 14.7 Transport in bulk according to Annex II ofMARPOL73/78 and the IBC Code Not applicable.

· UN "Model Regulation": -

* SECTION 15: Regulatory information

· 15.1 Safety, health and environmental regulations/legislation specific for the substance or mixture

· National regulations

· Water hazard class: Generally not hazardous for water.· 15.2 Chemical safety assessment: A Chemical Safety Assessment has not been carried out.

SECTION 16: Other informationThis information is based on our present knowledge. However, this shall not constitute a guarantee for any specific productfeatures and shall not establish a legally valid contractual relationship.

· Department issuing SDS: Environmental Health and Safety.(Contd. on page 6)

IE

Page 25: Product code Description€¦ · Printing date 26.06.2015 Revision: 22.06.2015 40.1.5 SECTION 1: Identification of the substance/mixture and of the company/undertaking · 1.1 Product

Page 6/6

Safety data sheetaccording to 1907/2006/EC, Article 31

Printing date 26.06.2015 Revision: 22.06.2015

Trade name: C. elegans 10X Wash Buffer

(Contd. of page 5)

40.1.5

· Contact:Life Science Group, Environmental Health and Safety, 2000 Alfred Nobel Drive, Hercules, California, 94547: 1(510) 741-1000

Diagnostic Group, Environmental Health and Safety, 4000 Alfred Nobel Drive, Hercules, California, 94547: 1(510) 724-7000· Abbreviations and acronyms:

ADR: Accord européen sur le transport des marchandises dangereuses par Route (European Agreement concerning the International Carriage ofDangerous Goods by Road)IMDG: International Maritime Code for Dangerous GoodsIATA: International Air Transport AssociationGHS: Globally Harmonised System of Classification and Labelling of ChemicalsEINECS: European Inventory of Existing Commercial Chemical SubstancesELINCS: European List of Notified Chemical SubstancesCAS: Chemical Abstracts Service (division of the American Chemical Society)

· * Data compared to the previous version altered. IE

Page 26: Product code Description€¦ · Printing date 26.06.2015 Revision: 22.06.2015 40.1.5 SECTION 1: Identification of the substance/mixture and of the company/undertaking · 1.1 Product

Page 1/6

Safety data sheetaccording to 1907/2006/EC, Article 31

Printing date 26.06.2015 Revision: 18.06.2015

40.1.5

SECTION 1: Identification of the substance/mixture and of the company/undertaking

· 1.1 Product identifier

· Trade name: E. Coli OP50-pBAD

· Article number: 10030881· 1.2 Relevant identified uses of the substance or mixture and uses advised againstNo further relevant information available.

· Application of the substance / the mixture Laboratory chemicals

· 1.3 Details of the supplier of the safety data sheet· Manufacturer/Supplier:Bio-Rad Laboratories S.r.l.Via Cellini, 18A20090 Segrate-MilanoItalyphone: 39-02-216091 fax: [email protected] / [email protected]

· Information department:Technical services, customer [email protected] / [email protected]

· 1.4 Emergency telephone number: 02-216091 - active during office hours for technical information

* SECTION 2: Hazards identification

· 2.1 Classification of the substance or mixture· Classification according to Regulation (EC) No 1272/2008Acute Tox. 4 H302 Harmful if swallowed.

· 2.2 Label elements· Labelling according to Regulation (EC) No 1272/2008The substance is classified and labelled according to the CLP regulation.

· Hazard pictograms

GHS07

· Signal word Warning

· Hazard-determining components of labelling:escherichia coli bacteria - freeze dried

· Hazard statementsH302 Harmful if swallowed.

· Precautionary statementsP264 Wash thoroughly after handling.P270 Do not eat, drink or smoke when using this product.P301+P312 IF SWALLOWED: Call a POISON CENTER/doctor if you feel unwell.P330 Rinse mouth.P501 Dispose of contents/container in accordance with local/regional/national/international regulations.

· 2.3 Other hazards· Results of PBT and vPvB assessment· PBT: Not applicable.

(Contd. on page 2) IE

Page 27: Product code Description€¦ · Printing date 26.06.2015 Revision: 22.06.2015 40.1.5 SECTION 1: Identification of the substance/mixture and of the company/undertaking · 1.1 Product

Page 2/6

Safety data sheetaccording to 1907/2006/EC, Article 31

Printing date 26.06.2015 Revision: 18.06.2015

Trade name: E. Coli OP50-pBAD

(Contd. of page 1)

40.1.5

· vPvB: Not applicable.

SECTION 3: Composition/information on ingredients

· 3.1 Chemical characterisation: Substances· CAS No. Description:escherichia coli bacteria - freeze dried

· Additonal information: For the wording of the listed risk phrases refer to section 16.

* SECTION 4: First aid measures

· 4.1 Description of first aid measures· After inhalation Supply fresh air; consult doctor in case of complaints.· After eye contact Rinse opened eye for several minutes under running water.· After swallowing Induce vomiting and call for medical help.· 4.2 Most important symptoms and effects, both acute and delayed No further relevant information available.· 4.3 Indication of any immediate medical attention and special treatment neededNo further relevant information available.

SECTION 5: Firefighting measures

· 5.1 Extinguishing media· Suitable extinguishing agentsCO2, extinguishing powder or water spray. Fight larger fires with water spray or alcohol resistant foam.

· 5.2 Special hazards arising from the substance or mixture No further relevant information available.· 5.3 Advice for firefighters· Protective equipment: No special measures required.

* SECTION 6: Accidental release measures

· 6.1 Personal precautions, protective equipment and emergency procedures Wear protective clothing.· 6.2 Environmental precautions: Do not allow to enter sewers/ surface or ground water.· 6.3 Methods and material for containment and cleaning up: Pick up mechanically.· 6.4 Reference to other sectionsSee Section 7 for information on safe handlingSee Section 8 for information on personal protection equipment.See Section 13 for disposal information.

* SECTION 7: Handling and storage

· 7.1 Precautions for safe handling No special precautions are necessary if used correctly.· Information about protection against explosions and fires: No special measures required.

· 7.2 Conditions for safe storage, including any incompatibilities· Storage· Requirements to be met by storerooms and receptacles: According to product specification· Information about storage in one common storage facility: Not required.

(Contd. on page 3) IE

Page 28: Product code Description€¦ · Printing date 26.06.2015 Revision: 22.06.2015 40.1.5 SECTION 1: Identification of the substance/mixture and of the company/undertaking · 1.1 Product

Page 3/6

Safety data sheetaccording to 1907/2006/EC, Article 31

Printing date 26.06.2015 Revision: 18.06.2015

Trade name: E. Coli OP50-pBAD

(Contd. of page 2)

40.1.5

· Further information about storage conditions: None.· 7.3 Specific end use(s) No further relevant information available.

* SECTION 8: Exposure controls/personal protection

· Additional information about design of technical systems: No further data; see item 7.

· 8.1 Control parameters· Components with limit values that require monitoring at the workplace: Not required.· Additional information: The lists that were valid during the creation were used as basis.

· 8.2 Exposure controls· Personal protective equipment· General protective and hygienic measures Wash hands before breaks and at the end of work.· Protection of hands: Protective gloves.· Material of gloves Synthetic gloves· Penetration time of glove materialThe exact break trough time has to be found out by the manufacturer of the protective gloves and has to be observed.

· Eye protection: Safety glasses

SECTION 9: Physical and chemical properties

· 9.1 Information on basic physical and chemical properties· General Information· Appearance:

Form: Solid in various formsColour: Light yellow

· Odour: Odourless· Odour threshold: Not determined.

· pH-value: Not applicable.

· Change in conditionMelting point/Melting range: undeterminedBoiling point/Boiling range: undetermined

· Flash point: Not applicable

· Flammability (solid, gaseous) Product is not flammable.

· Ignition temperature:

Decomposition temperature: Not determined.

· Self igniting: Not determined.

· Danger of explosion: Product does not present an explosion hazard.

· Explosion limits:Lower: Not determined.Upper: Not determined.

· Vapour pressure: Not applicable.

· Density: Not determined

(Contd. on page 4) IE

Page 29: Product code Description€¦ · Printing date 26.06.2015 Revision: 22.06.2015 40.1.5 SECTION 1: Identification of the substance/mixture and of the company/undertaking · 1.1 Product

Page 4/6

Safety data sheetaccording to 1907/2006/EC, Article 31

Printing date 26.06.2015 Revision: 18.06.2015

Trade name: E. Coli OP50-pBAD

(Contd. of page 3)

40.1.5

· Relative density Not determined.· Vapour density Not applicable.· Evaporation rate Not applicable.

· Solubility in / Miscibility withWater: Fully miscible

· Partition coefficient (n-octanol/water): Not determined.

· Viscosity:dynamic: Not applicable.kinematic: Not applicable.Organic solvents: 0,0 %

Solids content: 100,0 %· 9.2 Other information No further relevant information available.

SECTION 10: Stability and reactivity

· 10.1 Reactivity· 10.2 Chemical stability· Thermal decomposition / conditions to be avoided: No decomposition if used according to specifications.· 10.3 Possibility of hazardous reactions No dangerous reactions known· 10.4 Conditions to avoid No further relevant information available.· 10.5 Incompatible materials: No further relevant information available.· 10.6 Hazardous decomposition products: No dangerous decomposition products known

* SECTION 11: Toxicological information

· 11.1 Information on toxicological effects· Acute toxicity:· Primary irritant effect:· on the skin: No irritant effect.· Sensitisation: No sensitising effects known.

SECTION 12: Ecological information

· 12.1 Toxicity· Aquatic toxicity: No further relevant information available.· 12.2 Persistence and degradability No further relevant information available.· 12.3 Bioaccumulative potential No further relevant information available.· 12.4 Mobility in soil No further relevant information available.· Additional ecological information:· General notes:Water hazard class 1 (German Regulation) (Self-assessment): slightly hazardous for water.Do not allow undiluted product or large quantities of it to reach ground water, water course or sewage system.

· 12.5 Results of PBT and vPvB assessment· PBT: Not applicable.· vPvB: Not applicable.

(Contd. on page 5) IE

Page 30: Product code Description€¦ · Printing date 26.06.2015 Revision: 22.06.2015 40.1.5 SECTION 1: Identification of the substance/mixture and of the company/undertaking · 1.1 Product

Page 5/6

Safety data sheetaccording to 1907/2006/EC, Article 31

Printing date 26.06.2015 Revision: 18.06.2015

Trade name: E. Coli OP50-pBAD

(Contd. of page 4)

40.1.5

· 12.6 Other adverse effects No further relevant information available.

SECTION 13: Disposal considerations

· 13.1 Waste treatment methods· RecommendationMust not be disposed of together with household garbage. Do not allow product to reach sewage system.

· Uncleaned packagings:· Recommendation: Disposal must be made according to official regulations.

SECTION 14: Transport information

· 14.1 UN-Number· ADR, ADN, IMDG, IATA Void

· 14.2 UN proper shipping name· ADR, ADN, IMDG, IATA Void

· 14.3 Transport hazard class(es)

· ADR, ADN, IMDG, IATA· Class Void

· 14.4 Packing group· ADR, IMDG, IATA Void

· 14.5 Environmental hazards:· Marine pollutant: No

· 14.6 Special precautions for user Not applicable.

· 14.7 Transport in bulk according to Annex II ofMARPOL73/78 and the IBC Code Not applicable.

· UN "Model Regulation": -

* SECTION 15: Regulatory information

· 15.1 Safety, health and environmental regulations/legislation specific for the substance or mixture

· National regulations

· Water hazard class: Water hazard class 1 (Self-assessment): slightly hazardous for water.· 15.2 Chemical safety assessment: A Chemical Safety Assessment has not been carried out.

SECTION 16: Other informationThis information is based on our present knowledge. However, this shall not constitute a guarantee for any specific productfeatures and shall not establish a legally valid contractual relationship.

· Department issuing SDS: Environmental Health and Safety.(Contd. on page 6)

IE

Page 31: Product code Description€¦ · Printing date 26.06.2015 Revision: 22.06.2015 40.1.5 SECTION 1: Identification of the substance/mixture and of the company/undertaking · 1.1 Product

Page 6/6

Safety data sheetaccording to 1907/2006/EC, Article 31

Printing date 26.06.2015 Revision: 18.06.2015

Trade name: E. Coli OP50-pBAD

(Contd. of page 5)

40.1.5

· Contact:Life Science Group, Environmental Health and Safety, 2000 Alfred Nobel Drive, Hercules, California, 94547: 1(510) 741-1000

Diagnostic Group, Environmental Health and Safety, 4000 Alfred Nobel Drive, Hercules, California, 94547: 1(510) 724-7000· Abbreviations and acronyms:

ADR: Accord européen sur le transport des marchandises dangereuses par Route (European Agreement concerning the International Carriage ofDangerous Goods by Road)IMDG: International Maritime Code for Dangerous GoodsIATA: International Air Transport AssociationGHS: Globally Harmonised System of Classification and Labelling of ChemicalsCAS: Chemical Abstracts Service (division of the American Chemical Society)Acute Tox. 4: Acute toxicity, Hazard Category 4

· * Data compared to the previous version altered. IE

Page 32: Product code Description€¦ · Printing date 26.06.2015 Revision: 22.06.2015 40.1.5 SECTION 1: Identification of the substance/mixture and of the company/undertaking · 1.1 Product

Page 1/7

Safety data sheetaccording to 1907/2006/EC, Article 31

Printing date 26.06.2015 Revision: 22.06.2015

40.1.5

SECTION 1: Identification of the substance/mixture and of the company/undertaking

· 1.1 Product identifier

· Trade name: Cholesterol Solution

· Article number: 10030938· 1.2 Relevant identified uses of the substance or mixture and uses advised againstNo further relevant information available.

· Application of the substance / the mixture Laboratory chemicals

· 1.3 Details of the supplier of the safety data sheet· Manufacturer/Supplier:Bio-Rad Laboratories S.r.l.Via Cellini, 18A20090 Segrate-MilanoItalyphone: 39-02-216091 fax: [email protected] / [email protected]

· Information department:Technical services, customer [email protected] / [email protected]

· 1.4 Emergency telephone number: 02-216091 - active during office hours for technical information

* SECTION 2: Hazards identification

· 2.1 Classification of the substance or mixture· Classification according to Regulation (EC) No 1272/2008Flam. Liq. 2 H225 Highly flammable liquid and vapour.

· 2.2 Label elements· Labelling according to Regulation (EC) No 1272/2008The product is classified and labelled according to the CLP regulation.

· Hazard pictograms

GHS02

· Signal word Danger· Hazard statementsH225 Highly flammable liquid and vapour.

· Precautionary statementsP210 Keep away from heat/sparks/open flames/hot surfaces. - No smoking.P241 Use explosion-proof electrical/ventilating/lighting/equipment.P280 Wear protective gloves/protective clothing/eye protection/face protection.P240 Ground/bond container and receiving equipment.P303+P361+P353 IF ON SKIN (or hair): Remove/Take off immediately all contaminated clothing. Rinse skin with water/

shower.P501 Dispose of contents/container in accordance with local/regional/national/international regulations.

· 2.3 Other hazards· Results of PBT and vPvB assessment· PBT: Not applicable.

(Contd. on page 2) IE

Page 33: Product code Description€¦ · Printing date 26.06.2015 Revision: 22.06.2015 40.1.5 SECTION 1: Identification of the substance/mixture and of the company/undertaking · 1.1 Product

Page 2/7

Safety data sheetaccording to 1907/2006/EC, Article 31

Printing date 26.06.2015 Revision: 22.06.2015

Trade name: Cholesterol Solution

(Contd. of page 1)

40.1.5

· vPvB: Not applicable.

* SECTION 3: Composition/information on ingredients

· 3.2 Chemical characterisation: Mixtures· Description: Mixture of the substances listed below with nonhazardous additions.· Dangerous components:CAS: 64-17-5EINECS: 200-578-6

ethanol Flam. Liq. 2, H225 50-100%

· Additional information For the wording of the listed risk phrases refer to section 16.

SECTION 4: First aid measures

· 4.1 Description of first aid measures· After inhalation Supply fresh air; consult doctor in case of complaints.· After eye contact Rinse opened eye for several minutes under running water.· After swallowing Induce vomiting and call for medical help.· 4.2 Most important symptoms and effects, both acute and delayed No further relevant information available.· 4.3 Indication of any immediate medical attention and special treatment neededNo further relevant information available.

* SECTION 5: Firefighting measures

· 5.1 Extinguishing media· Suitable extinguishing agentsCO2, extinguishing powder or water spray. Fight larger fires with water spray or alcohol resistant foam.

· 5.2 Special hazards arising from the substance or mixture No further relevant information available.· 5.3 Advice for firefighters· Protective equipment: No special measures required.

* SECTION 6: Accidental release measures

· 6.1 Personal precautions, protective equipment and emergency procedures Wear protective clothing.· 6.2 Environmental precautions: Do not allow to enter sewers/ surface or ground water.· 6.3 Methods and material for containment and cleaning up:Absorb with liquid-binding material (sand, diatomite, acid binders, universal binders, sawdust).

· 6.4 Reference to other sectionsSee Section 7 for information on safe handlingSee Section 8 for information on personal protection equipment.See Section 13 for disposal information.

* SECTION 7: Handling and storage

· 7.1 Precautions for safe handling No special precautions are necessary if used correctly.· Information about protection against explosions and fires: No special measures required.

(Contd. on page 3) IE

Page 34: Product code Description€¦ · Printing date 26.06.2015 Revision: 22.06.2015 40.1.5 SECTION 1: Identification of the substance/mixture and of the company/undertaking · 1.1 Product

Page 3/7

Safety data sheetaccording to 1907/2006/EC, Article 31

Printing date 26.06.2015 Revision: 22.06.2015

Trade name: Cholesterol Solution

(Contd. of page 2)

40.1.5

· 7.2 Conditions for safe storage, including any incompatibilities· Storage· Requirements to be met by storerooms and receptacles: According to product specification· Information about storage in one common storage facility: Not required.· Further information about storage conditions: None.· 7.3 Specific end use(s) No further relevant information available.

* SECTION 8: Exposure controls/personal protection

· Additional information about design of technical systems: No further data; see item 7.

· 8.1 Control parameters· Components with limit values that require monitoring at the workplace:

64-17-5 ethanolTWA (Italy, (Italian)) Short-term value: 1000 ppm

A3· Additional information: The lists that were valid during the creation were used as basis.

· 8.2 Exposure controls· Personal protective equipment· General protective and hygienic measures Wash hands before breaks and at the end of work.· Breathing equipment: Not required.· Protection of hands: Protective gloves.· Material of gloves Synthetic gloves· Penetration time of glove materialThe exact break trough time has to be found out by the manufacturer of the protective gloves and has to be observed.

· Eye protection: Safety glasses

SECTION 9: Physical and chemical properties

· 9.1 Information on basic physical and chemical properties· General Information· Appearance:

Form: LiquidColour: Colourless

· Odour: Alcohol-like· Odour threshold: Not determined.

· pH-value: Not determined.

· Change in conditionMelting point/Melting range: undeterminedBoiling point/Boiling range: 78 °C

· Flash point: 21 °C

· Flammability (solid, gaseous) Not applicable.

· Ignition temperature: 425 °C

(Contd. on page 4) IE

Page 35: Product code Description€¦ · Printing date 26.06.2015 Revision: 22.06.2015 40.1.5 SECTION 1: Identification of the substance/mixture and of the company/undertaking · 1.1 Product

Page 4/7

Safety data sheetaccording to 1907/2006/EC, Article 31

Printing date 26.06.2015 Revision: 22.06.2015

Trade name: Cholesterol Solution

(Contd. of page 3)

40.1.5

· Decomposition temperature: Not determined.

· Self igniting: Product is not selfigniting.

· Danger of explosion: Product is not explosive. However, formation of explosive air/vapour mixtures arepossible.

· Explosion limits:Lower: 3,5 Vol %Upper: 15,0 Vol %

· Vapour pressure at 20 °C: 59 hPa

· Density: Not determined· Relative density Not determined.· Vapour density Not determined.· Evaporation rate Not determined.

· Solubility in / Miscibility withWater: Fully miscible

· Partition coefficient (n-octanol/water): Not determined.

· Viscosity:dynamic: Not determined.kinematic: Not determined.

· Solvent content:Organic solvents: 91,0 %

Solids content: 9,0 %· 9.2 Other information No further relevant information available.

SECTION 10: Stability and reactivity

· 10.1 Reactivity· 10.2 Chemical stability· Thermal decomposition / conditions to be avoided: No decomposition if used according to specifications.· 10.3 Possibility of hazardous reactions No dangerous reactions known· 10.4 Conditions to avoid No further relevant information available.· 10.5 Incompatible materials: No further relevant information available.· 10.6 Hazardous decomposition products: No dangerous decomposition products known

* SECTION 11: Toxicological information

· 11.1 Information on toxicological effects· Acute toxicity:· LD/LC50 values that are relevant for classification:

64-17-5 ethanolOral LD50 7060 mg/kg (rat)Inhalative LC50/4 h 20000 mg/l (rat)

(Contd. on page 5) IE

Page 36: Product code Description€¦ · Printing date 26.06.2015 Revision: 22.06.2015 40.1.5 SECTION 1: Identification of the substance/mixture and of the company/undertaking · 1.1 Product

Page 5/7

Safety data sheetaccording to 1907/2006/EC, Article 31

Printing date 26.06.2015 Revision: 22.06.2015

Trade name: Cholesterol Solution

(Contd. of page 4)

40.1.5

· Primary irritant effect:· on the skin: No irritant effect.· on the eye: Irritating effect.· Sensitisation: No sensitising effects known.

SECTION 12: Ecological information

· 12.1 Toxicity· Aquatic toxicity: No further relevant information available.· 12.2 Persistence and degradability No further relevant information available.· 12.3 Bioaccumulative potential No further relevant information available.· 12.4 Mobility in soil No further relevant information available.· Additional ecological information:· General notes:Water hazard class 1 (German Regulation) (Self-assessment): slightly hazardous for water.Do not allow undiluted product or large quantities of it to reach ground water, water course or sewage system.

· 12.5 Results of PBT and vPvB assessment· PBT: Not applicable.· vPvB: Not applicable.· 12.6 Other adverse effects No further relevant information available.

SECTION 13: Disposal considerations

· 13.1 Waste treatment methods· RecommendationMust not be disposed of together with household garbage. Do not allow product to reach sewage system.

· Uncleaned packagings:· Recommendation: Disposal must be made according to official regulations.

SECTION 14: Transport information

· 14.1 UN-Number· ADR, IMDG, IATA UN1170

· 14.2 UN proper shipping name· ADR 1170 ETHANOL (ETHYL ALCOHOL), mixture· IMDG ETHANOL (ETHYL ALCOHOL), mixture· IATA ETHANOL, mixture

· 14.3 Transport hazard class(es)

· ADR, IMDG, IATA· Class 3 Flammable liquids.· Label 3

· 14.4 Packing group· ADR, IMDG, IATA II

(Contd. on page 6) IE

Page 37: Product code Description€¦ · Printing date 26.06.2015 Revision: 22.06.2015 40.1.5 SECTION 1: Identification of the substance/mixture and of the company/undertaking · 1.1 Product

Page 6/7

Safety data sheetaccording to 1907/2006/EC, Article 31

Printing date 26.06.2015 Revision: 22.06.2015

Trade name: Cholesterol Solution

(Contd. of page 5)

40.1.5

· 14.5 Environmental hazards:· Marine pollutant: No

· 14.6 Special precautions for user Warning: Flammable liquids.· Danger code (Kemler): 33· EMS Number: F-E,S-D

· 14.7 Transport in bulk according to Annex II ofMARPOL73/78 and the IBC Code Not applicable.

· Transport/Additional information:

· ADR· Limited quantities (LQ) 1L· Transport category 2 · Tunnel restriction code D/E

· UN "Model Regulation": UN1170, ETHANOL (ETHYL ALCOHOL), mixture, 3, II

* SECTION 15: Regulatory information

· 15.1 Safety, health and environmental regulations/legislation specific for the substance or mixture

· National regulations

· Technical instructions (air):

Class Share in %

NK 50-100

· Water hazard class: Water hazard class 1 (Self-assessment): slightly hazardous for water.· 15.2 Chemical safety assessment: A Chemical Safety Assessment has not been carried out.

SECTION 16: Other informationThis information is based on our present knowledge. However, this shall not constitute a guarantee for any specific productfeatures and shall not establish a legally valid contractual relationship.

· Relevant phrasesH225 Highly flammable liquid and vapour.

· Department issuing SDS: Environmental Health and Safety.· Contact:Life Science Group, Environmental Health and Safety, 2000 Alfred Nobel Drive, Hercules, California, 94547: 1(510) 741-1000

Diagnostic Group, Environmental Health and Safety, 4000 Alfred Nobel Drive, Hercules, California, 94547: 1(510) 724-7000· Abbreviations and acronyms:

ADR: Accord européen sur le transport des marchandises dangereuses par Route (European Agreement concerning the International Carriage ofDangerous Goods by Road)IMDG: International Maritime Code for Dangerous GoodsIATA: International Air Transport AssociationGHS: Globally Harmonised System of Classification and Labelling of ChemicalsEINECS: European Inventory of Existing Commercial Chemical SubstancesELINCS: European List of Notified Chemical Substances

(Contd. on page 7) IE

Page 38: Product code Description€¦ · Printing date 26.06.2015 Revision: 22.06.2015 40.1.5 SECTION 1: Identification of the substance/mixture and of the company/undertaking · 1.1 Product

Page 7/7

Safety data sheetaccording to 1907/2006/EC, Article 31

Printing date 26.06.2015 Revision: 22.06.2015

Trade name: Cholesterol Solution

(Contd. of page 6)

40.1.5

CAS: Chemical Abstracts Service (division of the American Chemical Society)LC50: Lethal concentration, 50 percentLD50: Lethal dose, 50 percentFlam. Liq. 2: Flammable liquids, Hazard Category 2

· * Data compared to the previous version altered. IE

Page 39: Product code Description€¦ · Printing date 26.06.2015 Revision: 22.06.2015 40.1.5 SECTION 1: Identification of the substance/mixture and of the company/undertaking · 1.1 Product

Page 1/6

Safety data sheetaccording to 1907/2006/EC, Article 31

Printing date 26.06.2015 Revision: 26.06.2015

40.1.5

SECTION 1: Identification of the substance/mixture and of the company/undertaking

· 1.1 Product identifier

· Trade name: Ampicillin, lyophilized

· Article number: 1660407, 1660407EDU, 9702928· CAS Number:69-52-3

· EC number:200-708-1

· 1.2 Relevant identified uses of the substance or mixture and uses advised againstNo further relevant information available.

· Application of the substance / the mixture Laboratory chemicals

· 1.3 Details of the supplier of the safety data sheet· Manufacturer/Supplier:Bio-Rad Laboratories S.r.l.Via Cellini, 18A20090 Segrate-MilanoItalyphone: 39-02-216091 fax: [email protected] / [email protected]

· Information department:Technical services, customer [email protected] / [email protected]

· 1.4 Emergency telephone number: 02-216091 - active during office hours for technical information

* SECTION 2: Hazards identification

· 2.1 Classification of the substance or mixture· Classification according to Regulation (EC) No 1272/2008Skin Irrit. 2 H315 Causes skin irritation.Eye Irrit. 2 H319 Causes serious eye irritation.STOT SE 3 H335 May cause respiratory irritation.

· 2.2 Label elements· Labelling according to Regulation (EC) No 1272/2008The substance is classified and labelled according to the CLP regulation.

· Hazard pictograms

GHS07

· Signal word Warning

· Hazard-determining components of labelling:sodium [2S-[2alpha,5alpha,6ß(S*)]]-6-(aminophenylacetamido)-3,3-dimethyl-7-oxo-4-thia-1-azabicyclo[3.2.0]heptane-2-carboxylate

· Hazard statementsH315 Causes skin irritation.H319 Causes serious eye irritation.H335 May cause respiratory irritation.

(Contd. on page 2) IE

Page 40: Product code Description€¦ · Printing date 26.06.2015 Revision: 22.06.2015 40.1.5 SECTION 1: Identification of the substance/mixture and of the company/undertaking · 1.1 Product

Page 2/6

Safety data sheetaccording to 1907/2006/EC, Article 31

Printing date 26.06.2015 Revision: 26.06.2015

Trade name: Ampicillin, lyophilized

(Contd. of page 1)

40.1.5

· Precautionary statementsP261 Avoid breathing dust/fume/gas/mist/vapours/spray.P280 Wear protective gloves/protective clothing/eye protection/face protection.P305+P351+P338 IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy

to do. Continue rinsing.P362 Take off contaminated clothing and wash before reuse.P405 Store locked up.P501 Dispose of contents/container in accordance with local/regional/national/international regulations.

· 2.3 Other hazards· Results of PBT and vPvB assessment· PBT: Not applicable.· vPvB: Not applicable.

SECTION 3: Composition/information on ingredients

· 3.1 Chemical characterisation: Substances· CAS No. Description:69-52-3 sodium [2S-[2alpha,5alpha,6ß(S*)]]-6- (aminophenylacetamido)-3,3-dimethyl-7-oxo-4-thia-1- azabicyclo[3.2.0]heptane-2-carboxylate

· Identification number(s):· EC number: 200-708-1· Additonal information: For the wording of the listed risk phrases refer to section 16.

* SECTION 4: First aid measures

· 4.1 Description of first aid measures· After inhalation Supply fresh air; consult doctor in case of complaints.· After eye contact Rinse opened eye for several minutes under running water.· After swallowing Induce vomiting and call for medical help.· 4.2 Most important symptoms and effects, both acute and delayed No further relevant information available.· 4.3 Indication of any immediate medical attention and special treatment neededNo further relevant information available.

* SECTION 5: Firefighting measures

· 5.1 Extinguishing media· Suitable extinguishing agentsCO2, extinguishing powder or water spray. Fight larger fires with water spray or alcohol resistant foam.

· 5.2 Special hazards arising from the substance or mixture No further relevant information available.· 5.3 Advice for firefighters· Protective equipment: No special measures required.

* SECTION 6: Accidental release measures

· 6.1 Personal precautions, protective equipment and emergency procedures Wear protective clothing.· 6.2 Environmental precautions: No special measures required.

(Contd. on page 3) IE

Page 41: Product code Description€¦ · Printing date 26.06.2015 Revision: 22.06.2015 40.1.5 SECTION 1: Identification of the substance/mixture and of the company/undertaking · 1.1 Product

Page 3/6

Safety data sheetaccording to 1907/2006/EC, Article 31

Printing date 26.06.2015 Revision: 26.06.2015

Trade name: Ampicillin, lyophilized

(Contd. of page 2)

40.1.5

· 6.3 Methods and material for containment and cleaning up: Pick up mechanically.· 6.4 Reference to other sectionsSee Section 7 for information on safe handlingSee Section 8 for information on personal protection equipment.See Section 13 for disposal information.

* SECTION 7: Handling and storage

· 7.1 Precautions for safe handling No special measures required.· Information about protection against explosions and fires: No special measures required.

· 7.2 Conditions for safe storage, including any incompatibilities· Storage· Requirements to be met by storerooms and receptacles: According to product specification· Information about storage in one common storage facility: Not required.· Further information about storage conditions: None.· 7.3 Specific end use(s) No further relevant information available.

* SECTION 8: Exposure controls/personal protection

· Additional information about design of technical systems: No further data; see item 7.

· 8.1 Control parameters· Components with limit values that require monitoring at the workplace: Not required.· Additional information: The lists that were valid during the creation were used as basis.

· 8.2 Exposure controls· Personal protective equipment· General protective and hygienic measures Wash hands before breaks and at the end of work.· Protection of hands: Protective gloves.· Material of gloves Synthetic gloves· Penetration time of glove materialThe exact break trough time has to be found out by the manufacturer of the protective gloves and has to be observed.

· Eye protection: Safety glasses

SECTION 9: Physical and chemical properties

· 9.1 Information on basic physical and chemical properties· General Information· Appearance:

Form: CrystallineColour: Light yellow

· Odour: Odourless· Odour threshold: Not determined.

· pH-value: Not applicable.

· Change in conditionMelting point/Melting range: 240 °C

(Contd. on page 4) IE

Page 42: Product code Description€¦ · Printing date 26.06.2015 Revision: 22.06.2015 40.1.5 SECTION 1: Identification of the substance/mixture and of the company/undertaking · 1.1 Product

Page 4/6

Safety data sheetaccording to 1907/2006/EC, Article 31

Printing date 26.06.2015 Revision: 26.06.2015

Trade name: Ampicillin, lyophilized

(Contd. of page 3)

40.1.5

Boiling point/Boiling range: undetermined

· Flash point: Not applicable

· Flammability (solid, gaseous) Product is not flammable.

· Ignition temperature:

Decomposition temperature: Not determined.

· Self igniting: Not determined.

· Danger of explosion: Product does not present an explosion hazard.

· Explosion limits:Lower: Not determined.Upper: Not determined.

· Vapour pressure: Not applicable.

· Density: Not determined· Relative density Not determined.· Vapour density Not applicable.· Evaporation rate Not applicable.

· Solubility in / Miscibility withWater: Fully miscible

· Partition coefficient (n-octanol/water): Not determined.

· Viscosity:dynamic: Not applicable.kinematic: Not applicable.Organic solvents: 0,0 %

Solids content: 100,0 %· 9.2 Other information No further relevant information available.

SECTION 10: Stability and reactivity

· 10.1 Reactivity· 10.2 Chemical stability· Thermal decomposition / conditions to be avoided: No decomposition if used according to specifications.· 10.3 Possibility of hazardous reactions No dangerous reactions known· 10.4 Conditions to avoid No further relevant information available.· 10.5 Incompatible materials: No further relevant information available.· 10.6 Hazardous decomposition products: No dangerous decomposition products known

* SECTION 11: Toxicological information

· 11.1 Information on toxicological effects· Acute toxicity:· Primary irritant effect:· on the skin: Irritant to skin and mucous membranes.· on the eye: Irritating effect.

(Contd. on page 5) IE

Page 43: Product code Description€¦ · Printing date 26.06.2015 Revision: 22.06.2015 40.1.5 SECTION 1: Identification of the substance/mixture and of the company/undertaking · 1.1 Product

Page 5/6

Safety data sheetaccording to 1907/2006/EC, Article 31

Printing date 26.06.2015 Revision: 26.06.2015

Trade name: Ampicillin, lyophilized

(Contd. of page 4)

40.1.5

· Sensitisation: No sensitising effects known.

SECTION 12: Ecological information

· 12.1 Toxicity· Aquatic toxicity: No further relevant information available.· 12.2 Persistence and degradability No further relevant information available.· 12.3 Bioaccumulative potential No further relevant information available.· 12.4 Mobility in soil No further relevant information available.· Additional ecological information:· General notes: Generally not hazardous for water.· 12.5 Results of PBT and vPvB assessment· PBT: Not applicable.· vPvB: Not applicable.· 12.6 Other adverse effects No further relevant information available.

SECTION 13: Disposal considerations

· 13.1 Waste treatment methods· RecommendationHand over to hazardous waste disposers.Must not be disposed of together with household garbage. Do not allow product to reach sewage system.

· Uncleaned packagings:· Recommendation: Disposal must be made according to official regulations.· Recommended cleansing agent: Water, if necessary with cleansing agents.

SECTION 14: Transport information

· 14.1 UN-Number· ADR, ADN, IMDG, IATA Void

· 14.2 UN proper shipping name· ADR, ADN, IMDG, IATA Void

· 14.3 Transport hazard class(es)

· ADR, ADN, IMDG, IATA· Class Void

· 14.4 Packing group· ADR, IMDG, IATA Void

· 14.5 Environmental hazards:· Marine pollutant: No

· 14.6 Special precautions for user Not applicable.

· 14.7 Transport in bulk according to Annex II ofMARPOL73/78 and the IBC Code Not applicable.

(Contd. on page 6) IE

Page 44: Product code Description€¦ · Printing date 26.06.2015 Revision: 22.06.2015 40.1.5 SECTION 1: Identification of the substance/mixture and of the company/undertaking · 1.1 Product

Page 6/6

Safety data sheetaccording to 1907/2006/EC, Article 31

Printing date 26.06.2015 Revision: 26.06.2015

Trade name: Ampicillin, lyophilized

(Contd. of page 5)

40.1.5

· UN "Model Regulation": -

* SECTION 15: Regulatory information

· 15.1 Safety, health and environmental regulations/legislation specific for the substance or mixture

· National regulations

· Water hazard class: Generally not hazardous for water.· 15.2 Chemical safety assessment: A Chemical Safety Assessment has not been carried out.

SECTION 16: Other informationThis information is based on our present knowledge. However, this shall not constitute a guarantee for any specific productfeatures and shall not establish a legally valid contractual relationship.

· Department issuing SDS: Environmental Health and Safety.· Contact:Life Science Group, Environmental Health and Safety, 2000 Alfred Nobel Drive, Hercules, California, 94547: 1(510) 741-1000

Diagnostic Group, Environmental Health and Safety, 4000 Alfred Nobel Drive, Hercules, California, 94547: 1(510) 724-7000· Abbreviations and acronyms:

ADR: Accord européen sur le transport des marchandises dangereuses par Route (European Agreement concerning the International Carriage ofDangerous Goods by Road)IMDG: International Maritime Code for Dangerous GoodsIATA: International Air Transport AssociationGHS: Globally Harmonised System of Classification and Labelling of ChemicalsEINECS: European Inventory of Existing Commercial Chemical SubstancesCAS: Chemical Abstracts Service (division of the American Chemical Society)Skin Irrit. 2: Skin corrosion/irritation, Hazard Category 2Eye Irrit. 2: Serious eye damage/eye irritation, Hazard Category 2STOT SE 3: Specific target organ toxicity - Single exposure, Hazard Category 3

· * Data compared to the previous version altered. IE

Page 45: Product code Description€¦ · Printing date 26.06.2015 Revision: 22.06.2015 40.1.5 SECTION 1: Identification of the substance/mixture and of the company/undertaking · 1.1 Product

Page 1/6

Safety data sheetaccording to 1907/2006/EC, Article 31

Printing date 26.06.2015 Revision: 22.06.2015

40.1.5

SECTION 1: Identification of the substance/mixture and of the company/undertaking

· 1.1 Product identifier

· Trade name: C. elegans Wild / Mutant

· Article number: 10030879, 10030880· 1.2 Relevant identified uses of the substance or mixture and uses advised againstNo further relevant information available.

· Application of the substance / the mixture Laboratory chemicals

· 1.3 Details of the supplier of the safety data sheet· Manufacturer/Supplier:Bio-Rad Laboratories S.r.l.Via Cellini, 18A20090 Segrate-MilanoItalyphone: 39-02-216091 fax: [email protected] / [email protected]

· Information department:Technical services, customer [email protected] / [email protected]

· 1.4 Emergency telephone number: 02-216091 - active during office hours for technical information

* SECTION 2: Hazards identification

· 2.1 Classification of the substance or mixture· Classification according to Regulation (EC) No 1272/2008Skin Irrit. 2 H315 Causes skin irritation.Eye Irrit. 2 H319 Causes serious eye irritation.

· 2.2 Label elements· Labelling according to Regulation (EC) No 1272/2008The product is classified and labelled according to the CLP regulation.

· Hazard pictograms

GHS07

· Signal word Warning· Hazard statementsH315 Causes skin irritation.H319 Causes serious eye irritation.

· Precautionary statementsP280 Wear protective gloves/protective clothing/eye protection/face protection.P264 Wash thoroughly after handling.P305+P351+P338 IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy

to do. Continue rinsing.P362 Take off contaminated clothing and wash before reuse.P332+P313 If skin irritation occurs: Get medical advice/attention.P337+P313 If eye irritation persists: Get medical advice/attention.

(Contd. on page 2) IE

Page 46: Product code Description€¦ · Printing date 26.06.2015 Revision: 22.06.2015 40.1.5 SECTION 1: Identification of the substance/mixture and of the company/undertaking · 1.1 Product

Page 2/6

Safety data sheetaccording to 1907/2006/EC, Article 31

Printing date 26.06.2015 Revision: 22.06.2015

Trade name: C. elegans Wild / Mutant

(Contd. of page 1)

40.1.5

· 2.3 Other hazards· Results of PBT and vPvB assessment· PBT: Not applicable.· vPvB: Not applicable.

* SECTION 3: Composition/information on ingredients

· 3.2 Chemical characterisation: Mixtures· Description: Mixture of the substances listed below with nonhazardous additions.· Dangerous components:CAS: 7647-14-5EINECS: 231-598-3

sodium chloride Skin Irrit. 2, H315; Eye Irrit. 2, H319 20-35%

CAS: 56-81-5EINECS: 200-289-5

glycerol substance with a Community workplace exposure limit 1,0-2,5%

· Additional information For the wording of the listed risk phrases refer to section 16.

* SECTION 4: First aid measures

· 4.1 Description of first aid measures· After inhalation Supply fresh air; consult doctor in case of complaints.· After eye contact Rinse opened eye for several minutes under running water.· After swallowing Induce vomiting and call for medical help.· 4.2 Most important symptoms and effects, both acute and delayed No further relevant information available.· 4.3 Indication of any immediate medical attention and special treatment neededNo further relevant information available.

SECTION 5: Firefighting measures

· 5.1 Extinguishing media· Suitable extinguishing agentsCO2, extinguishing powder or water spray. Fight larger fires with water spray or alcohol resistant foam.

· 5.2 Special hazards arising from the substance or mixture No further relevant information available.· 5.3 Advice for firefighters· Protective equipment: No special measures required.

* SECTION 6: Accidental release measures

· 6.1 Personal precautions, protective equipment and emergency procedures Wear protective clothing.· 6.2 Environmental precautions:Dilute with plenty of water.Do not allow to enter sewers/ surface or ground water.

· 6.3 Methods and material for containment and cleaning up:Absorb with liquid-binding material (sand, diatomite, acid binders, universal binders, sawdust).

· 6.4 Reference to other sectionsSee Section 7 for information on safe handlingSee Section 8 for information on personal protection equipment.

(Contd. on page 3) IE

Page 47: Product code Description€¦ · Printing date 26.06.2015 Revision: 22.06.2015 40.1.5 SECTION 1: Identification of the substance/mixture and of the company/undertaking · 1.1 Product

Page 3/6

Safety data sheetaccording to 1907/2006/EC, Article 31

Printing date 26.06.2015 Revision: 22.06.2015

Trade name: C. elegans Wild / Mutant

(Contd. of page 2)

40.1.5

See Section 13 for disposal information.

SECTION 7: Handling and storage

· 7.1 Precautions for safe handling No special precautions are necessary if used correctly.· Information about protection against explosions and fires: No special measures required.

· 7.2 Conditions for safe storage, including any incompatibilities· Storage· Requirements to be met by storerooms and receptacles: According to product specification· Information about storage in one common storage facility: Not required.· Further information about storage conditions: None.· 7.3 Specific end use(s) No further relevant information available.

* SECTION 8: Exposure controls/personal protection

· Additional information about design of technical systems: No further data; see item 7.

· 8.1 Control parameters· Components with limit values that require monitoring at the workplace:

56-81-5 glycerolTWA (Italy, (Italian)) 10 mg/m³

· Additional information: The lists that were valid during the creation were used as basis.

· 8.2 Exposure controls· Personal protective equipment· General protective and hygienic measures Wash hands before breaks and at the end of work.· Breathing equipment: Not required.· Protection of hands: Protective gloves.· Material of gloves Synthetic gloves· Penetration time of glove materialThe exact break trough time has to be found out by the manufacturer of the protective gloves and has to be observed.

· Eye protection: Safety glasses

SECTION 9: Physical and chemical properties

· 9.1 Information on basic physical and chemical properties· General Information· Appearance:

Form: LiquidColour: Clear

· Odour: Odourless· Odour threshold: Not determined.

· pH-value at 20 °C: 6

· Change in conditionMelting point/Melting range: undetermined

(Contd. on page 4) IE

Page 48: Product code Description€¦ · Printing date 26.06.2015 Revision: 22.06.2015 40.1.5 SECTION 1: Identification of the substance/mixture and of the company/undertaking · 1.1 Product

Page 4/6

Safety data sheetaccording to 1907/2006/EC, Article 31

Printing date 26.06.2015 Revision: 22.06.2015

Trade name: C. elegans Wild / Mutant

(Contd. of page 3)

40.1.5

Boiling point/Boiling range: undetermined

· Flash point: Not applicable

· Flammability (solid, gaseous) Not applicable.

· Ignition temperature:

Decomposition temperature: Not determined.

· Self igniting: Product is not selfigniting.

· Danger of explosion: Product does not present an explosion hazard.

· Explosion limits:Lower: Not determined.Upper: Not determined.

· Vapour pressure at 20 °C: 23 hPa

· Density at 20 °C: 1,18849 g/cm³· Relative density Not determined.· Vapour density Not determined.· Evaporation rate Not determined.

· Solubility in / Miscibility withWater: Fully miscible

· Partition coefficient (n-octanol/water): Not determined.

· Viscosity:dynamic: Not determined.kinematic: Not determined.

· Solvent content:Organic solvents: 1,3 %Water: 39,3 %

Solids content: 59,4 %· 9.2 Other information No further relevant information available.

SECTION 10: Stability and reactivity

· 10.1 Reactivity· 10.2 Chemical stability· Thermal decomposition / conditions to be avoided: No decomposition if used according to specifications.· 10.3 Possibility of hazardous reactions No dangerous reactions known· 10.4 Conditions to avoid No further relevant information available.· 10.5 Incompatible materials: No further relevant information available.· 10.6 Hazardous decomposition products: No dangerous decomposition products known

IE

(Contd. on page 5)

Page 49: Product code Description€¦ · Printing date 26.06.2015 Revision: 22.06.2015 40.1.5 SECTION 1: Identification of the substance/mixture and of the company/undertaking · 1.1 Product

Page 5/6

Safety data sheetaccording to 1907/2006/EC, Article 31

Printing date 26.06.2015 Revision: 22.06.2015

Trade name: C. elegans Wild / Mutant

(Contd. of page 4)

40.1.5

* SECTION 11: Toxicological information

· 11.1 Information on toxicological effects· Acute toxicity:· Primary irritant effect:· on the skin: Irritant to skin and mucous membranes.· on the eye: Irritating effect.· Sensitisation: No sensitising effects known.

SECTION 12: Ecological information

· 12.1 Toxicity· Aquatic toxicity: No further relevant information available.· 12.2 Persistence and degradability No further relevant information available.· 12.3 Bioaccumulative potential No further relevant information available.· 12.4 Mobility in soil No further relevant information available.· Additional ecological information:· General notes:Water hazard class 1 (German Regulation) (Self-assessment): slightly hazardous for water.Do not allow undiluted product or large quantities of it to reach ground water, water course or sewage system.

· 12.5 Results of PBT and vPvB assessment· PBT: Not applicable.· vPvB: Not applicable.· 12.6 Other adverse effects No further relevant information available.

SECTION 13: Disposal considerations

· 13.1 Waste treatment methods· RecommendationMust not be disposed of together with household garbage. Do not allow product to reach sewage system.

· Uncleaned packagings:· Recommendation: Disposal must be made according to official regulations.· Recommended cleansing agent: Water, if necessary with cleansing agents.

SECTION 14: Transport information

· 14.1 UN-Number· ADR, ADN, IMDG, IATA Void

· 14.2 UN proper shipping name· ADR, ADN, IMDG, IATA Void

· 14.3 Transport hazard class(es)

· ADR, ADN, IMDG, IATA· Class Void

(Contd. on page 6) IE

Page 50: Product code Description€¦ · Printing date 26.06.2015 Revision: 22.06.2015 40.1.5 SECTION 1: Identification of the substance/mixture and of the company/undertaking · 1.1 Product

Page 6/6

Safety data sheetaccording to 1907/2006/EC, Article 31

Printing date 26.06.2015 Revision: 22.06.2015

Trade name: C. elegans Wild / Mutant

(Contd. of page 5)

40.1.5

· 14.4 Packing group· ADR, IMDG, IATA Void

· 14.5 Environmental hazards:· Marine pollutant: No

· 14.6 Special precautions for user Not applicable.

· 14.7 Transport in bulk according to Annex II ofMARPOL73/78 and the IBC Code Not applicable.

· UN "Model Regulation": -

* SECTION 15: Regulatory information

· 15.1 Safety, health and environmental regulations/legislation specific for the substance or mixture

· National regulations

· Technical instructions (air):

Class Share in %

NK 1.0-2.5

· Water hazard class: Water hazard class 1 (Self-assessment): slightly hazardous for water.· 15.2 Chemical safety assessment: A Chemical Safety Assessment has not been carried out.

SECTION 16: Other informationThis information is based on our present knowledge. However, this shall not constitute a guarantee for any specific productfeatures and shall not establish a legally valid contractual relationship.

· Relevant phrasesH315 Causes skin irritation.H319 Causes serious eye irritation.

· Department issuing SDS: Environmental Health and Safety.· Contact:Life Science Group, Environmental Health and Safety, 2000 Alfred Nobel Drive, Hercules, California, 94547: 1(510) 741-1000

Diagnostic Group, Environmental Health and Safety, 4000 Alfred Nobel Drive, Hercules, California, 94547: 1(510) 724-7000· Abbreviations and acronyms:

ADR: Accord européen sur le transport des marchandises dangereuses par Route (European Agreement concerning the International Carriage ofDangerous Goods by Road)IMDG: International Maritime Code for Dangerous GoodsIATA: International Air Transport AssociationGHS: Globally Harmonised System of Classification and Labelling of ChemicalsEINECS: European Inventory of Existing Commercial Chemical SubstancesELINCS: European List of Notified Chemical SubstancesCAS: Chemical Abstracts Service (division of the American Chemical Society)Skin Irrit. 2: Skin corrosion/irritation, Hazard Category 2Eye Irrit. 2: Serious eye damage/eye irritation, Hazard Category 2

· * Data compared to the previous version altered. IE